Veterinary parasitology, regional studies and reports最新文献

{"title":"Prevalence and molecular characterization of Ascaridia galli in chickens from Minoufiya Governorate, Egypt","authors":"Mahmoud AbouLaila ,&nbsp;Maram Mahmoud ,&nbsp;Heba Wheeb ,&nbsp;Makoto Igarashi ,&nbsp;Ahmed Elkhtam ,&nbsp;Soad Menshawy","doi":"10.1016/j.vprsr.2024.101170","DOIUrl":"10.1016/j.vprsr.2024.101170","url":null,"abstract":"<div><div><em>Ascaridia galli</em> causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of <em>A. galli</em> in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of <em>A. galli</em>. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of <em>A. galli</em> in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of <em>A. galli</em> in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of <em>A. galli</em> from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as <em>A. galli</em> from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of <em>A. galli</em> in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of <em>A. galli</em> epidemiology in this district and developing effective control strategies.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101170"},"PeriodicalIF":1.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bat ectoparasites (Diptera: Streblidae and Acari: Spinturnicidae) from an urban area in the Amazon-Cerrado transition","authors":"Alan Eriksson ,&nbsp;Sergio Gomes da Silva ,&nbsp;Francimeire Fernandes Ferreira","doi":"10.1016/j.vprsr.2024.101175","DOIUrl":"10.1016/j.vprsr.2024.101175","url":null,"abstract":"<div><div>Parasites significantly influence ecosystems by controlling host populations and spreading diseases, thereby impacting ecological balances. In the Neotropics, hematophagous bat flies and mites are common ectoparasites of bats. The state of Mato Grosso, Brazil, hosts a diverse bat fauna across its Amazon Forest, Cerrado, and Pantanal habitats. We described bat flies and wing mites associated with bats in an urban area in the Amazon-Cerrado transition. Despite the expected diversity of bat in the study area, we expect to find a low number of ectoparasites species, as urban areas are typically associated with reduced parasite diversity. Bats were captured using mist nets, and ectoparasites collected manually or with tweezers and identified under the stereomicroscope. We found 10 species of ectoparasites (seven bat flies and three wingmites) from 181 bats across 22 species. The study found low ectoparasite diversity, possibly due to the small number of bats captured or the constraints found in the urban environment. Most observed bat-ectoparasite associations were consistent with previous studies, but some unexpected associations suggested possible contamination or transient relationships. This research fills a gap in the knowledge of bat ectoparasites in Mato Grosso, contributing to understanding bat-ectoparasite dynamics in diverse habitats.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101175"},"PeriodicalIF":1.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ursicoptes americanus infestation of American black bears (Ursus americanus) in Virginia, USA","authors":"Jillian Broadhurst ,&nbsp;Raquel Francisco ,&nbsp;Peach Van Wick ,&nbsp;Karra Pierce ,&nbsp;David McRuer ,&nbsp;Ernesto Dominguez ,&nbsp;Leigh-Ann Horne ,&nbsp;Jess Ransier ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.vprsr.2024.101172","DOIUrl":"10.1016/j.vprsr.2024.101172","url":null,"abstract":"<div><div>American black bears (<em>Ursus americanus)</em> admitted to a Virginia rehabilitation center were tested for <em>Ursicoptes americanus</em>. From 2014 to 2023, 23 of 150 (15.3 %) bears were positive with higher prevalence in juveniles and females. Several bears had localized or multifocal, moderate lesions or alopecia consistent with mange; however, some had no clinical signs associated with mange. Several bears were co-infested with <em>Sarcoptes scabiei</em>. These data show that infestation with <em>U. americanus</em> mites is common and does not always cause disease. However, there is a need to understand potential mange-causing mites in black bears, especially because sarcoptic mange cases continue to increase in eastern bear populations.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101172"},"PeriodicalIF":1.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasmal meningoencephalitis in a 14 week-old shelter cat with presumed feline infectious peritonitis","authors":"Amira Abdu ,&nbsp;M. Erin Henry ,&nbsp;Nicole M. André ,&nbsp;Andrew D. Miller ,&nbsp;Manigandan Lejeune ,&nbsp;Elizabeth A. Berliner ,&nbsp;Gary R. Whittaker","doi":"10.1016/j.vprsr.2024.101173","DOIUrl":"10.1016/j.vprsr.2024.101173","url":null,"abstract":"<div><div>Toxoplasmal meningoencephalitis is a sporadic condition that is often misdiagnosed antemortem, frequently resulting in euthanasia especially in resource-limited settings. Here we report a case of a 7-week-old female domestic shorthair cat from an animal shelter who presented in a compromised condition and continued to display clinical signs consistent with a “failure to thrive” kitten. Weight loss and decreased activity were observed, and later on, neurological dysfunction became apparent. Based on clinical signs, non-effusive feline infectious peritonitis (FIP) was strongly suspected. Other possible conditions, such as a portosystemic shunt or toxoplasmosis, were also considered. Due to a poor prognosis, euthanasia was elected. Molecular testing at necropsy revealed no evidence for feline coronavirus infection; however, histopathological examination of the brain tissue revealed disseminated necrotizing meningoencephalitis with the presence of <em>Toxoplasma gondii</em> bradyzoites and tachyzoites, which was further confirmed by PCR and sequence analysis. In hindsight, it became clear that the kitten's failure to thrive was due to severe neurological compromise caused by overwhelming toxoplasmosis infection. This report underscores the diagnostic challenges associated with feline neurological disorders.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101173"},"PeriodicalIF":1.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and molecular characterization of Cryptosporidium oocysts in chickens from Egypt, and a meta-analysis for Cryptosporidium infections in chickens worldwide","authors":"Ahmed Essam ,&nbsp;Bassem Elmishmishy ,&nbsp;Enas Hammad ,&nbsp;Salah Abu Elwafa ,&nbsp;Ibrahim Abbas","doi":"10.1016/j.vprsr.2024.101169","DOIUrl":"10.1016/j.vprsr.2024.101169","url":null,"abstract":"<div><div><em>Cryptosporidium</em> is a leading cause of diarrhea in children and immunocompromised patients. Various animals and birds can also be infected with this protist, and <em>Cryptosporidium</em> zoonosis is common. A few reports have been published worldwide on <em>Cryptosporidium</em> infections in chickens. The present study surveyed <em>Cryptosporidium</em> infections in chickens from Dakahlia governorate, Egypt. <em>Cryptosporidium</em> oocysts were tested, using the Ziehl Neelsen modified acid-fast staining, in 884 chickens collected from various farms and live bird markets (LBMs) in Dakahlia governorate, Egypt. COWP (<em>Cryptosporidium</em> oocyst wall protein)-PCR testing coupled with isolate sequencing was conducted for 10 <em>Cryptosporidium</em>-positive samples. Intestinal contents of 58 (6.6 %) out of the surveyed chickens in Dakahlia, Egypt, had <em>Cryptosporidium</em> oocysts. The native breed (Balady) had a significantly higher prevalence than the commercial breeds. Oocysts of two different size ranges were detected; 4–5.5 × 4–5 μm and 6–7 × 5–6 μm. Two of the ten PCR-tested samples were successfully sequenced and identified as <em>Cryptosporidium parvum</em>. The present study conducted also the first meta-analysis describing the epidemiology, clinical disease, and species diversity of <em>Cryptosporidium</em> infecting chickens worldwide. The pooled <em>Cryptosporidium</em> prevalence in 18,579 chickens tested worldwide was 18.3 %, with insignificant geographical variations. The prevalence was two-fold higher (40.5 %) at the flock level. Chickens collected from LBMs (30.5 %) had significantly higher pooled prevalence than farmed chickens (14.4 %). Insignificant prevalence variations were detected according to the production type (broiler or layer), diagnostic method, or disease status. Three <em>Cryptosporidium</em> species common to infect birds were molecularly identified in 760 chickens tested worldwide: <em>Cryptosporidium baileyi</em>, <em>Cryptosporidium meleagridis</em>, and <em>Cryptosporidium galli</em>, with the former being the most frequently detected. <em>Cryptosporidium parvum</em> was detected in a few samples mostly from free-range chickens. In conclusion, potential <em>C. parvum</em> zoonosis from chickens is suggested in Egypt, where <em>C. parvum</em> is common among humans and various animals. Given that chicken has a substantial role in the global <em>C. meleagridis</em> zoonoses, and no bird-specific <em>Cryptosporidium</em> spp. were detected in the tested samples from Egypt, we do recommend a large-scale molecular survey to detect various species and genotypes infecting chickens throughout various Egyptian governorates.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101169"},"PeriodicalIF":1.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and characterization of Cryptosporidium spp. in the domestic cat (Felis catus): First description of C. felis in the state of Goiás and C. muris in the Midwest region of Brazil","authors":"Leda Margarita Castaño Barrios ,&nbsp;Lorrayne Honorio de Paula ,&nbsp;Fernando Borges Bosso ,&nbsp;Vando Edésio Soares ,&nbsp;Marcelo Vasconcelos Meireles ,&nbsp;Dunya Mara Cardoso Moraes ,&nbsp;Guido Fontgalland Coelho Linhares ,&nbsp;Jenner Karlisson Pimenta dos Reis ,&nbsp;Weslen Fabricio Pires Teixeira ,&nbsp;Valéria de Sá Jayme","doi":"10.1016/j.vprsr.2024.101167","DOIUrl":"10.1016/j.vprsr.2024.101167","url":null,"abstract":"<div><div>With the increasingly close coexistence between pets and humans, coupled with reports of <em>Cryptosporidium</em> cross-infection between felines and their guardians, research aimed at understanding the epidemiology of these parasites in specific regions has become necessary. The principal objective of this study was to undertake a molecular characterization of <em>Cryptosporidium</em> in fecal samples from felines in the state of Goiás, Brazil (which is unprecedented for the region), and to assess the risk factors involved in the infection of these animals in the aforementioned locale. A total of 95 fecal samples were collected from domestic felines in the municipality of Goiânia, Goiás, Brazil. All the animals involved were categorized by sex (male or female), lifestyle (stray or domiciled), and age (under six months or six months and older). After collection, all fecal aliquots underwent genomic DNA extraction, followed by a nested PCR (nPCR) technique targeting the 18 s rRNA gene. Subsequently, sequencing of all amplified products was performed to diagnose <em>Cryptosporidium</em> parasites. In addition to searching for <em>Cryptosporidium</em> in feces, all felines underwent serological testing for Feline Immunodeficiency Virus (FIV) antibodies (IgG) using the Enzyme-Linked Immunosorbent Assay (ELISA). Of all the fecal samples analyzed, 17.9 % (17/95) tested positive for the presence of <em>Cryptosporidium</em> spp. DNA, with the variable “age under six months” being the only statistically significant risk factor (<em>p</em> ≤ 0.05) for feline infection. <em>C. felis</em> was the most prevalent species, identified in 88.2 % (15/17) of the positive samples. <em>C. muris</em> was the second most diagnosed species, confirmed in 5.9 % (1/17) of the positive samples. Of all the feline serological samples evaluated, 48.42 % (46/95) were positive for anti-FIV IgG. It was confirmed that 75 % (3/4) of all adult animals positive for <em>Cryptosporidium</em> showed positive (50.00 % [2/4]) or indeterminate (25.00 % [1/4]) results for FIV. This unprecedented study provides the first report of parasites from the genus <em>Cryptosporidium</em> in domestic felines in the state of Goiás, including the first report of <em>C. felis</em> in the state and of <em>C. muris</em> in Midwest Brazil.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101167"},"PeriodicalIF":1.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiological survey of Babesia species infecting cattle in Paraguay","authors":"Claudia Esther Silvera Rojas ,&nbsp;Thillaiampalam Sivakumar ,&nbsp;Ngigi Noel Muthoni Mumbi ,&nbsp;Believe Ahedor ,&nbsp;Maria Fátima Rodríguez Valinotti ,&nbsp;Tomás Javier Acosta ,&nbsp;Naoaki Yokoyama","doi":"10.1016/j.vprsr.2024.101162","DOIUrl":"10.1016/j.vprsr.2024.101162","url":null,"abstract":"<div><div>Paraguay, an agricultural country in South America, has a high prevalence of tick infestations in its cattle population due to warm temperatures, high humidity, and extensive grazing management practices. Consequently, <em>Babesia</em> infections, which are transmitted by ticks, might have a wide distribution in Paraguay, but the current status of these infections remains uncertain. Therefore, we aimed to assess the infection status of three clinically significant <em>Babesia</em> species, including <em>Babesia bovis</em>, <em>Babesia bigemina</em>, and <em>Babesia naoakii</em>, among cattle populations in Paraguay. Blood samples were collected from a total of 326 cattle across nine departments in Paraguay, and their DNAs were screened with species-specific PCR assays. Of the surveyed cattle, single infections of <em>B. bovis</em> and <em>B. bigemina</em> were detected in 24 (7.4 %) and 127 (39.0 %), respectively, while co-infection with both the parasite species was detected in 38 (11.7 %). In contrast, all of the surveyed cattle were negative for <em>B. naoakii</em> infection. The prevalence of <em>B. bigemina</em>-single infection was higher in the Eastern region (49.0 %) compared to the Western region (34.6 %), in cattle under extensive management (51.3 %) compared to those under semi-intensive management (34.6 %), and in <em>Bos indicus</em> cattle (50.3 %) compared to <em>Bos taurus</em> (15.8 %). Our findings demonstrated a wide distribution of <em>B. bovis</em> and <em>B. bigemina</em> infections among cattle in Paraguay, underscoring the importance of implementing effective control measures to reduce the potential economic losses associated with bovine babesiosis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101162"},"PeriodicalIF":1.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-season survey of ixodid tick species collected from domestic dogs in Chad, Africa","authors":"Christopher A. Cleveland ,&nbsp;Morgan Friedman ,&nbsp;Alec T. Thompson ,&nbsp;Ellen Haynes ,&nbsp;Sarah M. Coker ,&nbsp;John A. Bryan II ,&nbsp;Metinou Sidouin ,&nbsp;Philip Tchindebet Ouakou ,&nbsp;Bongo Nare Richard Ngandolo ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.vprsr.2024.101165","DOIUrl":"10.1016/j.vprsr.2024.101165","url":null,"abstract":"<div><div>Ticks are medically important vectors of pathogens, many of which are zoonotic or impact domestic animal and/or wildlife health. Climate change, landuse modifications, and increasing interactions between domestic animals, wildlife, and humans have resulted in changes in tick-host dynamics and the emergence of novel pathogens worldwide. Therefore, describing the host and geographic ranges of vector species is essential in assessing disease risk, especially in understudied areas, and should be conducted in a One Health context. In sub-Saharan Africa, previous work on ticks has focused primarily on those species most relevant to domestic livestock or humans, highlighting a significant knowledge gap concerning species of ticks that infest domestic animals in rural areas. The objective of this study was to investigate the species diversity of ticks on domestic dogs in rural areas of Chad, Africa. From 2019 to 2022, we collected 3412 ixodid ticks from 435 domestic dogs from 23 villages in Chad, Africa during both dry and wet seasons. Ticks were identified to species using morphological techniques and/or molecular analyses of the 16S rDNA, 12S rDNA, and cytochrome oxidase I gene regions. We identified 11 species of ticks from dogs including <em>Amblyomma variegatum</em>, an <em>Amblyomma marmoreum</em> complex species, <em>Haemaphysalis leachi,</em> a <em>Haemaphysalis</em> sp., <em>Hyalomma impressum, Hyalomma truncatum, Rhipicephalus decoloratus, Rhipicephalus guilhoni, Rhipicephalus muhsamae, Rhipicephalus linnaei</em> (=<em>R. sanguineus</em> ‘tropical lineage’), and a <em>Rhipicephalus</em> sp. Several of these tick species are known vectors for important canine and zoonotic pathogens and some are more commonly associated with cattle hosts. Our results show that sampling ticks from domestic dogs provides an opportunity to examine vectors that may be infesting domestic animals, agricultural animals, wildlife, and humans as hosts in an understudied area.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101165"},"PeriodicalIF":1.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Trypanosoma microti in Cricetid rodents from Querétaro, Mexico: Expanding the known distribution of Herpetosoma in the Mexican transition zone","authors":"Estefania Grostieta ,&nbsp;Carlos I. Miranda-Caballero ,&nbsp;Sokani Sánchez-Montes ,&nbsp;Pablo Colunga-Salas ,&nbsp;Carlos A. López González ,&nbsp;Kelly D. Valderas-Muñoz ,&nbsp;Gustavo Arciniega-Luna ,&nbsp;Marco Torres-Castro ,&nbsp;Gabriela Aguilar-Tipacamú","doi":"10.1016/j.vprsr.2024.101168","DOIUrl":"10.1016/j.vprsr.2024.101168","url":null,"abstract":"<div><div>Members of the subgenus <em>Herpetosoma</em> are specialized parasites of mammals of the order Lagomorpha and Rodentia. It is estimated that around 50 species within this subgenus are transmitted by several flea species. These parasites have recently gained attention from a public health perspective because several species have been reported to infect humans. Several species of the <em>Herpetosoma</em> subgenus have been recognized on the American continent, although there have been no reports from the Mexican Transition Zone. Therefore, the present study aimed to report the presence of a member of the <em>Herpetosoma</em> subgenus in cricetid rodents from Querétaro, Mexico.</div><div>A total of 17 rodents belonging to three species, <em>Peromyscus boylii</em>, <em>Peromyscus gratus</em>, and <em>Peromyscus melanophrys</em>, were collected. Genetic material was extracted using the Chelex 100 resin, followed by PCR and sequencing of a fragment of 800 bp of the <em>18S</em> ribosomal gene of the genus <em>Trypanosoma</em>. Out of these hosts, eight individuals of <em>P. gratus</em> (1♀), <em>P. melanophrys</em> (1♂), and <em>P. boylii</em> (3♂, 3♀) tested positive for <em>Trypanosoma microti</em> DNA, previously detected in the England and Japan. The present study, therefore, provides the first discovery of <em>T</em>. <em>microti</em> in Mexico, thereby increasing the known richness of species of the genus <em>Trypanosoma</em> in rodents from this country and the Mexican Transition Zone.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101168"},"PeriodicalIF":1.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological evidence of gregarines (Phylum: Apicomplexa) in Triatoma dimidiata, Chagas disease vector in Mexico: Rediscovering Machadoella triatomae and a new intestinal gregarine","authors":"Karla Y. Acosta-Viana ,&nbsp;Nohemi Cigarroa-Toledo ,&nbsp;Julian E. Garcia-Rejon ,&nbsp;Carlos M. Baak-Baak ,&nbsp;Karla C. Amaya-Guardia ,&nbsp;María F. Ocaña-Correa ,&nbsp;Isabel Y. Chel-Muñoz ,&nbsp;José I. Chan-Pérez","doi":"10.1016/j.vprsr.2024.101163","DOIUrl":"10.1016/j.vprsr.2024.101163","url":null,"abstract":"<div><div>The objective of this study was to morphologically characterize the structures suggestive of gregarines found in the feces of <em>Triatoma dimidiata</em> collected in five municipalities in Yucatan, Mexico. Three hundred ninety-six specimens of <em>T. dimidiata</em> collected during 2022 and 2023 were analyzed, of which 8.84 % (<em>n</em> = 35) showed evidence of infection by a gregarine in the intestine and 1.77 % (<em>n</em> = 7) of infection with vermiform gregarine in Malpighian tubules. With microscopic examination, it was possible to obtain measurements and micrographs that allowed the rediscovery of <em>Machadoella triatomae</em> (vermiform gregarine) as well as a potential new gregarine species not previously described in <em>T. dimidiata</em>, which, based on its morphological characteristics, could be another Schizogregarina of the genus <em>Caulleryella</em> (flask-shaped). Additionally, the dissections of some specimens helped correctly identify the site where these gregarines parasitized <em>T. dimidiata</em>. Furthermore, of the three staining techniques used in this study, Lugol's solution and Wright's stain provided greater contrast and were therefore useful for visualizing these microorganisms. Scanning electron microscopy of the new gregarine revealed the three-dimensional shape of merozoites inside and outside a ruptured meront in detail. This study provides morphological evidence of two gregarines (Phylum: Apicomplexa) parasitizing <em>Triatoma dimidiata</em> in multiple regions of Yucatan, Mexico. <em>M. triatomae</em> was isolated in Malpighian tubules, and another gregarine, possibly of the genus <em>Caulleryella</em>, was isolated in the intestine.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101163"},"PeriodicalIF":1.4,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}