Pub Date : 2024-11-01DOI: 10.1016/j.vprsr.2024.101155
Jaylon Vaughn , Haylie J. Brown , Anthony Ogunbadewa , Solomon Odemuyiwa , Deborah M. Anderson , Samniqueka J. Halsey
{"title":"Withdrawal notice to: ‘Detection of Amblyomma maculatum and Rickettsia parkeri in prairies of Central Missouri’","authors":"Jaylon Vaughn , Haylie J. Brown , Anthony Ogunbadewa , Solomon Odemuyiwa , Deborah M. Anderson , Samniqueka J. Halsey","doi":"10.1016/j.vprsr.2024.101155","DOIUrl":"10.1016/j.vprsr.2024.101155","url":null,"abstract":"","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101155"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.vprsr.2024.101156
Enrique Salazar-Grosskelwing , Roger I. Rodriguez-Vivas , Manuel E. Bolio-González , Dora Romero-Salas , Rodolfo Ramos-Beltrán , Alberto Solano-Barquero , Alicia Rojas
{"title":"Withdrawal notice to: “First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico” [Veterinary Parasitology Regional Studies and Reports 56 (2024) 101127]","authors":"Enrique Salazar-Grosskelwing , Roger I. Rodriguez-Vivas , Manuel E. Bolio-González , Dora Romero-Salas , Rodolfo Ramos-Beltrán , Alberto Solano-Barquero , Alicia Rojas","doi":"10.1016/j.vprsr.2024.101156","DOIUrl":"10.1016/j.vprsr.2024.101156","url":null,"abstract":"","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101156"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Corrigendum to “Comparative study of the anthelmintic activity of ethanolic and aqueous extracts of Atriplex halimus, the anti-echinococcal plant used in Algeria” [Veterinary Parasitology: Regional Studies and Reports 54 (2024)/ 101094]","authors":"Meryem Benmarce , Maria Celina Elissondo , AbdElkarim Laatamna , Assia Haif","doi":"10.1016/j.vprsr.2024.101139","DOIUrl":"10.1016/j.vprsr.2024.101139","url":null,"abstract":"","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101139"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.vprsr.2024.101149
Omid BehrouziNasab , Ali Moshaverinia , Farzin Kazemi Lifshagerd
Pediculosis caused by chewing lice may pose a threat to domesticated and wild birds' health. These ectoparasites can cause pruritus, poor feather condition and do weaken hosts making them susceptible to other infections. Species of the genus Saemundssonia which are called ecomorphic head lice parasitize a wide range of aquatic birds from the family Laridae. The present report describes a heavy infestation of yellow-legged gulls (Larus michahellis) with Saemundssonia species in Iran. Three yellow-legged gulls that were brought to Mashhad Birds Garden (northeastern Iran) were examined visually for infestation with any ectoparasites and infestation with lice on the head zone was observed. The lice were collected and after clearing in aqueous solution of potassium hydroxide were mounted on slides. The morphological characteristics were used to identify 85 collected lice from three examined birds and they were identified as Saemundssonia lari. The lice of this species parasitize a broad range of host species and it seems to be less host specific. Iran has many aquatic habitats and a few lakes that host many migratory birds annually. The accumulation of these birds in these habitats could spread lice infestations among themselves that negatively affect their health and welfare. Therefore, Periodic checks to identify ectoparasites and other pathogens of migratory birds are quite necessary.
{"title":"First report of Saemundssonia lari on yellow-legged gull (larus michahellis) from Iran","authors":"Omid BehrouziNasab , Ali Moshaverinia , Farzin Kazemi Lifshagerd","doi":"10.1016/j.vprsr.2024.101149","DOIUrl":"10.1016/j.vprsr.2024.101149","url":null,"abstract":"<div><div>Pediculosis caused by chewing lice may pose a threat to domesticated and wild birds' health. These ectoparasites can cause pruritus, poor feather condition and do weaken hosts making them susceptible to other infections. Species of the genus <em>Saemundssonia</em> which are called ecomorphic head lice parasitize a wide range of aquatic birds from the family <em>Laridae</em>. The present report describes a heavy infestation of yellow-legged gulls (<em>Larus michahellis</em>) with <em>Saemundssonia</em> species in Iran. Three yellow-legged gulls that were brought to Mashhad Birds Garden (northeastern Iran) were examined visually for infestation with any ectoparasites and infestation with lice on the head zone was observed. The lice were collected and after clearing in aqueous solution of potassium hydroxide were mounted on slides. The morphological characteristics were used to identify 85 collected lice from three examined birds and they were identified as <em>Saemundssonia lari</em>. The lice of this species parasitize a broad range of host species and it seems to be less host specific. Iran has many aquatic habitats and a few lakes that host many migratory birds annually. The accumulation of these birds in these habitats could spread lice infestations among themselves that negatively affect their health and welfare. Therefore, Periodic checks to identify ectoparasites and other pathogens of migratory birds are quite necessary.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101149"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ixodid ticks are important arthropods in medicine and veterinary science, posing a considerable threat to livestock in East Africa. A repeated cross-sectional study was conducted from March 2022 to June 2023 to explore the spatial distribution, prevalence, species diversity and burden of cattle ticks, and to investigate risk factors associated with tick infestation prevalence and burden in northwest Ethiopia. A total of 2528 cattle were randomly selected through multistage cluster sampling for tick inspection across 18 districts during both dry and wet seasons. Ticks samples were identified morphologically at the genus and species levels. Data were analysed using descriptive statistics and mixed-effect logistic regression models. In total, 22,698 adult ticks were found from 1604 cattle; of which 10,246 were collected for identification, and revealed ten tick species across three genera: Amblyomma, Rhipicephalus, and Hyalomma. The predominant species included A. variegatum (35.5 %), R. decoloratus (32.73 %), A. lepidum (10.64 %), and H. rufipes (6.85 %), while R. sanguineus and H. analoticum were below 1 %. Tick diversity was highest in Kafta-Humera, followed by Wolkait and Tsegede. The overall tick infestation prevalence was 63.45 %, with a mean tick burden of 8.98 ± 0.31 ticks per animal, both significantly impacted by agroecology, season, vegetation cover, management, and coexistence with other livestock species (p < 0.05). Infestation rates varied notably among districts, with Wolkait having the highest at 94.49 % and Wogera the lowest at 9.17 %. This study underscores the high prevalence of tick infestations and the diverse ixodid tick species of veterinary importance in the region, highlighting the need for effective tick management strategies. Studies are recommended to explore the epidemiological features of relevant tick-borne pathogens.
{"title":"Spatiotemporal distribution, species diversity, and burden of ixodid ticks on cattle in Northwest Ethiopia","authors":"Zewdu Seyoum Tarekegn , Alemayehu Eshetie , Getie Mulat , Marshet Bazezew , Moges Maru , Shimelis Dagnachew Nigatu , Sefinew Alemu Mekonnen , Wassie Molla","doi":"10.1016/j.vprsr.2024.101148","DOIUrl":"10.1016/j.vprsr.2024.101148","url":null,"abstract":"<div><div>Ixodid ticks are important arthropods in medicine and veterinary science, posing a considerable threat to livestock in East Africa. A repeated cross-sectional study was conducted from March 2022 to June 2023 to explore the spatial distribution, prevalence, species diversity and burden of cattle ticks, and to investigate risk factors associated with tick infestation prevalence and burden in northwest Ethiopia. A total of 2528 cattle were randomly selected through multistage cluster sampling for tick inspection across 18 districts during both dry and wet seasons. Ticks samples were identified morphologically at the genus and species levels. Data were analysed using descriptive statistics and mixed-effect logistic regression models. In total, 22,698 adult ticks were found from 1604 cattle; of which 10,246 were collected for identification, and revealed ten tick species across three genera: <em>Amblyomma</em>, <em>Rhipicephalus,</em> and <em>Hyalomma</em>. The predominant species included <em>A. variegatum</em> (35.5 %), <em>R. decoloratus</em> (32.73 %), <em>A. lepidum</em> (10.64 %), and <em>H. rufipes</em> (6.85 %), while <em>R. sanguineus</em> and <em>H. analoticum</em> were below 1 %. Tick diversity was highest in Kafta-Humera, followed by Wolkait and Tsegede. The overall tick infestation prevalence was 63.45 %, with a mean tick burden of 8.98 ± 0.31 ticks per animal, both significantly impacted by agroecology, season, vegetation cover, management, and coexistence with other livestock species (<em>p</em> < 0.05). Infestation rates varied notably among districts, with Wolkait having the highest at 94.49 % and Wogera the lowest at 9.17 %. This study underscores the high prevalence of tick infestations and the diverse ixodid tick species of veterinary importance in the region, highlighting the need for effective tick management strategies. Studies are recommended to explore the epidemiological features of relevant tick-borne pathogens.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101148"},"PeriodicalIF":1.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.vprsr.2024.101146
Rolf K. Schuster, Saritha Sivakumar, Jörg Kinne
Samples from 1,254 MacQueen's bustards (Chlamydotis macqueenii) were sent for parasitological examination to the department of parasitology of the Central Veterinary Research Laboratory between 2003 and 2022. The birds originated from sanctuaries where confiscated birds were kept but also from from zoos and private collections or were bred in captivity and released to conservation areas where they got injured or died for various reasons. The material consisted of mainly of intestines and faecal samples as well as heads, stomachs, muscles samples and cysts, isolated helminths, ecto-parasites and fly larvae. A total of 419 birds (33.4 %) were positive for parasites and the following endoparasites were found: Five protozoans (Eumonospora megafalconis, two species of the genus Sarcocystis, Trichomonas gallinae, unidentified intestinal flagellates), one trematode (Philophthalmus gralli), five cestodes (Ascometra vestita, Otiditaenia conoideis, Idiogenes otidis, Raillietina neyrai, Hispaniolepis falsata), six nematodes (Subuluridae sp., Hartertia rotundata, Histiocephalus skrjabini, Dispharynx nasuta, Synhimanthus sp., Paraspiralatus sakeri, unidentified encysted nematode larvae) and three acanthocephalans (Empodius taeniatus, Spherirostris embae, Centrorhynchus aluconis). The fauna of ectoparasites included the mallophage Otidoecus houbarae and the flea Echidnophaga gallinacea. In addition, fly maggots belonging to the family Sarcophagidae (Sarcophaga dux, Wohlfahrtia nuba) and to the Calliphoridae family (Chrysomya albiceps) were found on and in submitted houbara carcasses.
{"title":"Parasite findings in the MacQueen's bustard, Chlamydotis macqueenii (Grey, 1832), and considerations on the parasite fauna of bustards and the systematic position of some of the parasites","authors":"Rolf K. Schuster, Saritha Sivakumar, Jörg Kinne","doi":"10.1016/j.vprsr.2024.101146","DOIUrl":"10.1016/j.vprsr.2024.101146","url":null,"abstract":"<div><div>Samples from 1,254 MacQueen's bustards (<em>Chlamydotis macqueenii</em>) were sent for parasitological examination to the department of parasitology of the Central Veterinary Research Laboratory between 2003 and 2022. The birds originated from sanctuaries where confiscated birds were kept but also from from zoos and private collections or were bred in captivity and released to conservation areas where they got injured or died for various reasons. The material consisted of mainly of intestines and faecal samples as well as heads, stomachs, muscles samples and cysts, isolated helminths, ecto-parasites and fly larvae. A total of 419 birds (33.4 %) were positive for parasites and the following endoparasites were found: Five protozoans (<em>Eumonospora megafalconis,</em> two species of the genus <em>Sarcocystis, Trichomonas gallinae,</em> unidentified intestinal flagellates), one trematode (<em>Philophthalmus gralli</em>), five cestodes (<em>Ascometra vestita, Otiditaenia conoideis, Idiogenes otidis, Raillietina neyrai, Hispaniolepis falsata</em>), six nematodes (Subuluridae sp., <em>Hartertia rotundata, Histiocephalus skrjabini, Dispharynx nasuta, Synhimanthus</em> sp., <em>Paraspiralatus sakeri,</em> unidentified encysted nematode larvae) and three acanthocephalans (<em>Empodius taeniatus, Spherirostris embae, Centrorhynchus aluconis</em>). The fauna of ectoparasites included the mallophage <em>Otidoecus houbarae</em> and the flea <em>Echidnophaga gallinacea</em>. In addition, fly maggots belonging to the family Sarcophagidae (<em>Sarcophaga dux</em>, <em>Wohlfahrtia nuba)</em> and to the Calliphoridae family (<em>Chrysomya albiceps)</em> were found on and in submitted houbara carcasses.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101146"},"PeriodicalIF":1.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The contamination of soil by pathogenic protozoan parasites from both human and animal feces, is an important factor in the spread of parasitic infections globally. This contamination also serves as a crucial reservoir for zoonotic diseases, facilitating their transmission. In many developing nations, including India, there's limited documentation on the extent of soil contamination, which poses challenges for implementing effective One Health strategies aimed at controlling these infections.
Aim
To study the molecular prevalence and distribution pattern of pathogenic protozoa in soil from North India.
Methods
In this prospective cross-sectional study, we collected a total of 200 soil samples from various locations in the Chandigarh region, including public parks, urban slum areas, villages on the outskirts, and sites near cattle sheds, hospitals, and public school. DNA extraction was done in all the samples followed by conventional and nested PCR for the identification of Toxoplasma gondii, Entamoeba histolytica, Giardia duodenalis, and Cryptosporidium species from these samples.
Key results
Out of 200, 34 soil samples (17 %) from different sites in Chandigarh were found to have pathogenic protozoan contamination. Toxoplasma gondii was the most common protozoa (n = 18), followed by Entamoeba, Giardia, and Cryptosporidium species. Urban slums and public parks were most likely to be contaminated with oocysts/ cyst of pathogenic protozoa.
Conclusions
This study is the first of its kind in India and the prevalence of oocyst /cyst of protozoa in our city was found significant. The findings underscore the critical role of soil contamination by pathogenic protozoa as a potential source for spreading zoonotic infections.
{"title":"Molecular detection of potentially zoonotic protozoa in the Chandigarh region, India","authors":"Divya Rattan , Priya Datta , Devyani Sharma , Shikha Sharma , Rakesh Sehgal","doi":"10.1016/j.vprsr.2024.101143","DOIUrl":"10.1016/j.vprsr.2024.101143","url":null,"abstract":"<div><h3>Context</h3><div>The contamination of soil by pathogenic protozoan parasites from both human and animal feces, is an important factor in the spread of parasitic infections globally. This contamination also serves as a crucial reservoir for zoonotic diseases, facilitating their transmission. In many developing nations, including India, there's limited documentation on the extent of soil contamination, which poses challenges for implementing effective One Health strategies aimed at controlling these infections.</div></div><div><h3>Aim</h3><div>To study the molecular prevalence and distribution pattern of pathogenic protozoa in soil from North India.</div></div><div><h3>Methods</h3><div>In this prospective cross-sectional study, we collected a total of 200 soil samples from various locations in the Chandigarh region, including public parks, urban slum areas, villages on the outskirts, and sites near cattle sheds, hospitals, and public school. DNA extraction was done in all the samples followed by conventional and nested PCR for the identification of <em>Toxoplasma gondii</em>, <em>Entamoeba histolytica</em>, <em>Giardia duodenalis,</em> and <em>Cryptosporidium</em> species from these samples.</div></div><div><h3>Key results</h3><div>Out of 200, 34 soil samples (17 %) from different sites in Chandigarh were found to have pathogenic protozoan contamination. <em>Toxoplasma gondii</em> was the most common protozoa (<em>n</em> = 18), followed by <em>Entamoeba</em>, <em>Giardia</em>, and <em>Cryptosporidium</em> species<em>.</em> Urban slums and public parks were most likely to be contaminated with oocysts/ cyst of pathogenic protozoa.</div></div><div><h3>Conclusions</h3><div>This study is the first of its kind in India and the prevalence of oocyst /cyst of protozoa in our city was found significant. The findings underscore the critical role of soil contamination by pathogenic protozoa as a potential source for spreading zoonotic infections.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101143"},"PeriodicalIF":1.4,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-22DOI: 10.1016/j.vprsr.2024.101145
Abdul Rahman Kazim , Van Lun Low , Jamal Houssaini , Dennis Tappe , Chong Chin Heo
To shed light on the importance of tick-borne diseases, especially in farm animals that often contact with farm workers, this study aimed to identify ticks and tick-borne pathogens in ruminants in Malaysia. Accordingly, specimen collection was conducted across Peninsular Malaysia yielded a total of 1241 ticks collected from 674 farm ruminants. Among these, four tick species were identified, with Rhipicephalus microplus being the most prevalent, constituting 99.03 % of the total tick population. Analysis of 130 tick pools revealed three positives for Borrelia. BLAST analyses of the flaB and 16S rRNA genes revealed high similarities to Borrelia theileri, ranging from 98.78 to 100 % for flaB and 99.23–99.45 % for 16S rRNA. These results align with the phylogenetic trees, where sequences from both genes clustered together with B. theileri, further supporting this identification. No Rickettsia and Bartonella bacteria were detected. This study represents the first occurrence of B. theileri in R. microplus in Malaysia.
{"title":"Ticks and tick-borne pathogens in ruminant farms of Peninsular Malaysia: First molecular evidence of Borrelia theileri in Rhipicephalus microplus (Acari: Ixodidae).","authors":"Abdul Rahman Kazim , Van Lun Low , Jamal Houssaini , Dennis Tappe , Chong Chin Heo","doi":"10.1016/j.vprsr.2024.101145","DOIUrl":"10.1016/j.vprsr.2024.101145","url":null,"abstract":"<div><div>To shed light on the importance of tick-borne diseases, especially in farm animals that often contact with farm workers, this study aimed to identify ticks and tick-borne pathogens in ruminants in Malaysia. Accordingly, specimen collection was conducted across Peninsular Malaysia yielded a total of 1241 ticks collected from 674 farm ruminants. Among these, four tick species were identified, with <em>Rhipicephalus microplus</em> being the most prevalent, constituting 99.03 % of the total tick population. Analysis of 130 tick pools revealed three positives for <em>Borrelia</em>. BLAST analyses of the <em>flaB</em> and 16S rRNA genes revealed high similarities to <em>Borrelia theileri</em>, ranging from 98.78 to 100 % for <em>flaB</em> and 99.23–99.45 % for 16S rRNA. These results align with the phylogenetic trees, where sequences from both genes clustered together with <em>B. theileri</em>, further supporting this identification. No <em>Rickettsia</em> and <em>Bartonella</em> bacteria were detected. This study represents the first occurrence of <em>B. theileri</em> in <em>R. microplus</em> in Malaysia.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101145"},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-22DOI: 10.1016/j.vprsr.2024.101142
Seyma Gunyakti Kilinc , Harun Kaya Kesik , Figen Celik , Sami Simsek
Fascioliasis, caused by the parasite Fasciola hepatica, is a worldwide zoonotic disease that can have serious consequences for livestock, certain wild animals and humans. This study was conducted to morphologically and molecularly characterise a F. hepatica isolate from a brown bear. After examination of the internal organs, a Fasciola sp. isolate was obtained from the bile ducts of the liver. The adult parasite was morphologically analysed under a stereomicroscope and identified as F. hepatica. Measurements of body length, body width and ventral sucker area were then recorded. After isolation of the genomic DNA, a partial gene of the mitochondrial cytochrome oxidase subunit 1 (mt-CO1) was amplified by PCR. The amplified mt-CO1 PCR products were sequenced by one-way sequence analysis. According to the BLAST search results, the sequence of the isolate was identified as F. hepatica. In conclusion, this is the first report on the occurrence of F. hepatica in brown bears and the molecular characterisation of the isolate.
由肝脏法氏囊寄生虫引起的肝脏法氏囊病是一种世界性人畜共患病，可对家畜、某些野生动物和人类造成严重后果。本研究对从棕熊身上分离出的肝包虫进行了形态学和分子鉴定。在对内脏器官进行检查后，从肝脏胆管中分离出了一种 Fasciola sp.在体视显微镜下对成虫进行了形态分析，确定其为肝包虫。然后记录体长、体宽和腹吸盘面积。分离基因组 DNA 后，通过 PCR 扩增线粒体细胞色素氧化酶亚基 1（mt-CO1）的部分基因。扩增的 mt-CO1 PCR 产物通过单向序列分析进行测序。根据 BLAST 搜索结果，该分离株的序列被鉴定为肝吸虫。总之，这是首次报道棕熊中出现 F. hepatica 并对分离物进行分子鉴定。
{"title":"First report and molecular characterisation of an adult liver fluke (Fasciola hepatica) in a brown bear (Ursus arctos) in Türkiye","authors":"Seyma Gunyakti Kilinc , Harun Kaya Kesik , Figen Celik , Sami Simsek","doi":"10.1016/j.vprsr.2024.101142","DOIUrl":"10.1016/j.vprsr.2024.101142","url":null,"abstract":"<div><div>Fascioliasis, caused by the parasite <em>Fasciola hepatica</em>, is a worldwide zoonotic disease that can have serious consequences for livestock, certain wild animals and humans. This study was conducted to morphologically and molecularly characterise a <em>F. hepatica</em> isolate from a brown bear. After examination of the internal organs, a <em>Fasciola</em> sp. isolate was obtained from the bile ducts of the liver. The adult parasite was morphologically analysed under a stereomicroscope and identified as <em>F. hepatica</em>. Measurements of body length, body width and ventral sucker area were then recorded. After isolation of the genomic DNA, a partial gene of the mitochondrial cytochrome oxidase subunit 1 (mt-CO1) was amplified by PCR. The amplified mt-CO1 PCR products were sequenced by one-way sequence analysis. According to the BLAST search results, the sequence of the isolate was identified as <em>F. hepatica</em>. In conclusion, this is the first report on the occurrence of <em>F. hepatica</em> in brown bears and the molecular characterisation of the isolate.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101142"},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ectoparasites significantly impact the health and productivity of chickens, both directly and indirectly. From October 2022 to September 2023, a cross-sectional study was conducted in the East Gojam Zone of the Amhara region, North West Ethiopia to identifying the ectoparasite species infesting free-ranged chicken. Additionally, a questionnaire survey was used to assess farmers knowledge and attitudes towards control practices. The purposeful sampling method was utilized to select study districts and peasant associations (PAs), while the simple random sampling method was employed to select interviewed respondents (n = 90) and sampled chickens. Samples were collected from 384 chickens through visual examination of their entire bodies, including skin scraps, to expose any ectoparasites present. The ectoparasites were then identified according to diagnostic guidelines. Out of the 384 local chickens examined, 184 (47.9 %) were found to harbor one or more of ectoparasites. Four genera of ectoparasites were identified, with louse (Menacanthus stramineus) most prevalent species (N = 133/184) 72.3 % and fleas (Ceratophyllidae) being the least prevalent N = 3 (1.6 %). Lice species included Menacanthus stramineus (N = 98) 53.3 %, Lipeurus caponis (N= 15) 8.2 %, Goniocotes gallinae 7 (3.8 %), Menopon gallinae (N = 7) 3.8 %, and Cuclotogaster heterographus (N = 6) 3.3 %. Additionally, one species of ticks (Argas persicus, (N = 9) 4.9 %, mites (Knemidocoptes mutans, (N = 5) 2.7 %, and fleas (Echidnophaga gallinacean, (N = 3) 1.6 % were found. Statistically significant differences (p = 0.040) in prevalence were observed between age groups, breeds, management systems, and study areas. However, the prevalence was not statistically significant (p > 0.05) between sex categories. Results from the questionnaire survey revealed that most poultry farmers were female (N = 74) 82.2 % and illiterate (N = 52) 57.8 %. A majority of respondents (N = 55) 61.1 % lacked information regarding ectoparasite infestations in chickens, while (N = 36)36.7 % of respondents fed their chickens supplementary feed once a day. This study highlights the prevalence of ectoparasite infestations among chickens in the studied areas. Therefore, it is recommended to raise awareness among the community about control practices and conduct further studies focusing on the impacts of ectoparasites.
{"title":"Prevalence of domestic chicken ectoparasites in the east Goejam, Amhara, Ethiopia","authors":"Asnakew Mulaw Berihun , Yitayew Demessie Yekoye , Moges Maru Alemayehu","doi":"10.1016/j.vprsr.2024.101144","DOIUrl":"10.1016/j.vprsr.2024.101144","url":null,"abstract":"<div><div>Ectoparasites significantly impact the health and productivity of chickens, both directly and indirectly. From October 2022 to September 2023, a cross-sectional study was conducted in the East Gojam Zone of the Amhara region, North West Ethiopia to identifying the ectoparasite species infesting free-ranged chicken. Additionally, a questionnaire survey was used to assess farmers knowledge and attitudes towards control practices. The purposeful sampling method was utilized to select study districts and peasant associations (PAs), while the simple random sampling method was employed to select interviewed respondents (<em>n</em> = 90) and sampled chickens. Samples were collected from 384 chickens through visual examination of their entire bodies, including skin scraps, to expose any ectoparasites present. The ectoparasites were then identified according to diagnostic guidelines. Out of the 384 local chickens examined, 184 (47.9 %) were found to harbor one or more of ectoparasites. Four genera of ectoparasites were identified, with louse (<em>Menacanthus stramineus</em>) most prevalent species (<em>N</em> = 133/184) 72.3 % and fleas (<em>Ceratophyllidae)</em> being the least prevalent <em>N</em> = 3 (1.6 %). Lice species included <em>Menacanthus stramineus</em> (<em>N</em> = 98) 53.3 %, <em>Lipeurus caponis (N</em> <em>=</em> 15) 8.2 %, <em>Goniocotes gallinae</em> 7 (3.8 %), <em>Menopon gallinae</em> (<em>N</em> = 7) 3.8 %, and <em>Cuclotogaster heterographus</em> (<em>N</em> = 6) 3.3 %. Additionally, one species of ticks (<em>Argas persicus</em>, (<em>N</em> = 9) 4.9 %, mites (<em>Knemidocoptes mutans</em>, (<em>N</em> = 5) 2.7 %, and fleas (<em>Echidnophaga gallinacean</em>, (<em>N</em> = 3) 1.6 % were found. Statistically significant differences (<em>p</em> = 0.040) in prevalence were observed between age groups, breeds, management systems, and study areas. However, the prevalence was not statistically significant (<em>p</em> > 0.05) between sex categories. Results from the questionnaire survey revealed that most poultry farmers were female (<em>N</em> = 74) 82.2 % and illiterate (<em>N</em> = 52) 57.8 %. A majority of respondents (<em>N</em> = 55) 61.1 % lacked information regarding ectoparasite infestations in chickens, while (<em>N</em> = 36)36.7 % of respondents fed their chickens supplementary feed once a day. This study highlights the prevalence of ectoparasite infestations among chickens in the studied areas. Therefore, it is recommended to raise awareness among the community about control practices and conduct further studies focusing on the impacts of ectoparasites.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101144"},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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