Veterinary parasitology, regional studies and reports最新文献

{"title":"Pyrosequencing analysis of cytochrome b (CYTb) gene mutations in Babesia gibsoni: Correlation with drug resistance and treatment efficacy in South Korea","authors":"Galle Hettiarachchilage Ravindi Ashani Gunawardhana ,&nbsp;Doosung Cheon ,&nbsp;Woo-Jin Song ,&nbsp;Hyohoon Jeong ,&nbsp;YoungMin Yun","doi":"10.1016/j.vprsr.2025.101381","DOIUrl":"10.1016/j.vprsr.2025.101381","url":null,"abstract":"<div><div><em>Babesia gibsoni</em> is an intraerythrocytic tick-transmitted protozoan that presents a significant health risk to canine populations worldwide. Canine babesiosis is frequently associated with severe clinical symptoms, particularly hemolytic anemia. The present study aimed to determine the prevalence of mutations in the cytochrome <em>b</em> (<em>CYTb</em>) gene of <em>B. gibsoni</em> using pyrosequencing, and to explore potential correlations between specific mutations and treatment outcomes. The study was conducted at Jeju National University Animal Hospital, where canine patients diagnosed with <em>B. gibsoni</em> infection were evaluated. It is focused on five specific <em>CYTb</em> mutations: M121I, V220I, I303V, P152L, and F258Y. Genetic analysis using pyrosequencing was conducted on samples from infected dogs to identify the presence of these mutations. Various treatment regimens were administered based on clinical judgment, including atovaquone-azithromycin (AA), diminazene aceturate, metronidazole-clindamycin-doxycycline (MCD), and doxycycline-enrofloxacin-metronidazole (DEM). A total of 125 samples were used for mutation analysis, and only nine samples were assessed for the treatment response. The analysis revealed that the M121I mutation was present in 17 % of the <em>B. gibsoni</em>-positive dogs. Significant regional differences were observed in overall mutation prevalence among Jeju, Seoul and Gyeonggi area. Jeju samples showed a significantly higher mutation prevalence than those from PobAniLab. Dog sex, breed and concurrent disease were not significantly associated with the mutation prevalence. Overall, dogs with non-mutated strains tended to respond more favorably to initial treatments, whereas those with mutated strains often required more complex therapeutic approaches. This study supports the value of genetic monitoring in guiding treatment decisions and improving clinical outcomes of canine babesiosis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101381"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on anticoccidial drug resistance in Eimeria species of broilers from Kashmir, a North-Western Himalayan region","authors":"Shagufta Iqbal ,&nbsp;Syed Tanveer ,&nbsp;Idrees Mehraj Allaie ,&nbsp;Aiman Ashraf ,&nbsp;Bilal Ahmad Para","doi":"10.1016/j.vprsr.2025.101383","DOIUrl":"10.1016/j.vprsr.2025.101383","url":null,"abstract":"<div><div>This study represents the first report on anticoccidial drug resistance in <em>Eimeria</em> species infecting broiler chickens in Kashmir, North India, a region with unique temperate climatic conditions conducive to oocyst survival. A mixed population of field strains of <em>Eimeria</em> spp. from poultry farms in Kashmir Valley was evaluated to assess the current anticoccidial resistance status following World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines. The oocyst inoculum comprised of seven <em>Eimeria</em> species identified morphologically, of which five (<em>E. acervulina</em>, <em>E. maxima</em>, <em>E. mitis</em>, <em>E. praecox</em> and <em>E. tenella</em>) were confirmed molecularly using the ITS-1 rDNA marker. Forty, One-day-old Cobb-strain broilers, were randomly divided into four groups (<em>n</em> = 10 each): Group I (infected + amprolium treated), Group II (infected + sulphaquinoxaline treated), Group III (infected untreated control) and Group IV (uninfected control). Drug efficacy was determined by calculating the percentage global index (%GI) based on weight gain, feed conversion ratio, lesion score, oocyst index and mortality. The %GI values were 58.15 for sulphaquinoxaline-treated group and 56.86 for amprolium-treated group, indicating partial resistance, defined here as a moderate loss of drug efficacy without complete treatment failure. Morpho-Molecular analysis identified five <em>Eimeria</em> species in pre-treatment samples and detected the continued presence of four <em>Eimeria</em> species post-treatment, indicating potential emerging drug resistance. Phylogenetic analysis of ITS-1 sequences showed a close genetic affinity of local isolates with reference <em>Eimeria</em> strains deposited in GenBank and notably, this is the first report of ITS-1 sequences for <em>E. praecox</em> from Kashmir. In conclusion, the study provides the first molecularly supported evidence of anticoccidial resistance in broilers from Kashmir and underscores the need for routine monitoring, rotational use of anticoccidials and the adoption of integrated parasite control strategies to safeguard poultry productivity in the region.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101383"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute visceral cysticercosis in a Black Bengal goat: A case report of hepatitis cysticercosa and pneumonitis cysticercosa","authors":"Vinay Kumar S.D.,&nbsp;Sree Lakshmi P.,&nbsp;Neha,&nbsp;Avantika Srivastav,&nbsp;Manohar S.,&nbsp;Veena R. Sutar,&nbsp;Pawan Kumar,&nbsp;Rajveer Singh Pawaiya,&nbsp;Vidya Singh","doi":"10.1016/j.vprsr.2025.101378","DOIUrl":"10.1016/j.vprsr.2025.101378","url":null,"abstract":"<div><div>Cysticercosis is a parasitic infection caused by <em>Cysticercus,</em> the metacestode (larval stage) of <em>Taenia</em> spp. This case report describes the gross and histopathological findings of acute visceral cysticercosis associated with <em>Cysticercus tenuicollis</em> in a Black Bengal goat with a history of sudden recumbency and death. Striking gross lesions were observed in the liver, which displayed multifocal to coalescing, tortuous hemorrhagic cords of variable length throughout and over the surface, along with a substantial number of wandering immature cysticerci. In the lungs, well-demarcated, dark red, irregular areas of consolidation were observed distributed along the lateral margins of the pulmonary lobes on both sides. The cysts were 5–7 mm in length, containing clear, jelly-like fluid; under the microscope, a large invaginated scolex and a serrated vesicular wall were evident. Histopathology revealed a wide range of hepatic lesions, including cross-sections of vesicular walls of cysticerci within the distended portal veins, and the migratory tracts filled with blood, fibrin, and cellular debris. The zone of inflammatory cells around the cysticerci and their migratory tracts consisted of eosinophils, predominantly along with a few mononuclear cells. Cross sections of cysticerci were also seen in lung sections, exhibiting a similar inflammatory cell infiltrate as seen in the liver. Necropsy, histopathological, and parasitological findings confirmed the diagnosis as parasitic traumatic hepatitis (Hepatitis cysticercosa) and parasitic pneumonia (Pneumonitis cysticercosa), with the cause of death being hypovolemic shock caused by severe blood loss resulting from traumatic hepatitis and acute fibrinous peritonitis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101378"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and hematobiochemical changes associated with Toxoplasma gondii infection in goats from Khyber Pakhtunkhwa, Pakistan","authors":"Muhammad Iqbal ,&nbsp;Muhammad Yasin Tipu ,&nbsp;Asim Aslam ,&nbsp;Kamran Ashraf","doi":"10.1016/j.vprsr.2025.101373","DOIUrl":"10.1016/j.vprsr.2025.101373","url":null,"abstract":"<div><div>A cross-sectional study was conducted to determine the seroprevalence of <em>Toxoplasma gondii</em> (<em>T. gondii</em>) infection in goats and to evaluate associated haematological and biochemical alterations in Khyber Pakhtunkhwa, Pakistan. A total of 384 blood samples were randomly collected from goats in three districts and analyzed serologically using the latex agglutination test. The overall seroprevalence was 57.3 %, with the highest prevalence in Peshawar (60.2 %), followed by Dera Ismail Khan (57.8 %) and Swat (53.9 %). Higher infection rates were observed in female goats (66.1 %) and animals older than two years (71.4 %). Significant risk factors were female sex, age &gt; 2 years, grazing-based feeding, poor health status, and extensive rearing systems (<em>P</em> &lt; 0.05). Infected goats exhibited marked (P &lt; 0.05) haematological changes, including anemia, leukopenia, neutrophilia, and lymphocytopenia. Serum biochemistry revealed elevated liver enzymes (ALT, AST, LDH), as well as increased BUN and creatinine levels, alongside reduced albumin levels and increased total protein, indicating hepatic and renal involvement. The findings underscore the widespread occurrence of <em>T. gondii</em> infection in goats and its substantial impact on host physiology, with potential zoonotic implications. Priority interventions should focus on improving water hygiene, adopting controlled feeding systems, and implementing regular serological screening to effectively reduce <em>T. gondii</em> transmission and its impact on goat health.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101373"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145468174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of Macracanthorhynchus hirudinaceus (Pallas, 1781) in Didelphis marsupialis (Linnaeus, 1758) from the Brazilian Amazon","authors":"Lucas Araújo Ferreira,&nbsp;Ricardo Luis Sousa Santana,&nbsp;Elaine Lopes Carvalho,&nbsp;Elane Guerreiro Giese","doi":"10.1016/j.vprsr.2025.101388","DOIUrl":"10.1016/j.vprsr.2025.101388","url":null,"abstract":"<div><div>This study presents the first documented occurrence of <em>Macracanthorhynchus hirudinaceus</em> (Pallas, 1781) parasitizing <em>Didelphis marsupialis</em> (Linnaeus, 1758) in Brazil. Specimens were collected in the Soure Marine Extractive Reserve, Marajó Island, Pará state, northern Brazil. In January and February 2025, parasites were recovered from the intestinal region of two hosts. Necropsy revealed acanthocephalans of unusual sizes in the intestinal loops and significant granuloma formation. Additionally, fecal samples were collected from the cecum region. Morphological/morphometric analyses of the helminths and eggs identified in the feces suggest that the parasites are <em>Macracanthorhynchus hirudinaceus</em>. Thus, this study reports the first identification of <em>M. hirudinaceus</em> in <em>D. marsupialis</em> in the Brazilian Amazon, expanding the range of known hosts of this acanthocephalan.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101388"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of the giant kidney worm Dioctophyme renale in a domestic dog from Costa Rica","authors":"Fabián Villegas-Rojas ,&nbsp;Monserrat Barquero-Quesada ,&nbsp;Roberto W.I. Olivares ,&nbsp;Laura G. Bass ,&nbsp;Fernando Robles-Herrera ,&nbsp;Juan Carlos González-Barrantes ,&nbsp;Pablo J. Borrás ,&nbsp;Fernando A. Dittel-Meza ,&nbsp;Josué Campos-Camacho ,&nbsp;Alicia Rojas","doi":"10.1016/j.vprsr.2025.101387","DOIUrl":"10.1016/j.vprsr.2025.101387","url":null,"abstract":"<div><div><em>Dioctophyme renale</em>, the giant kidney worm, is a parasitic nematode of domestic and wild carnivores, occasionally infecting humans. While widely reported in South America, it has not been documented in Central America before. A 3-year-old French bulldog presented with leukocytosis, thrombocytopenia, azotemia, pyrexia, vomiting, and apathy. Abdominal ultrasound revealed tubular structures in the right kidney, and urine sediment contained nematode eggs. Euthanasia was performed after severe health complications, and a necropsy was done. The right kidney revealed severe hydronephrosis with parenchymal atrophy, fibrosis, and lymphoplasmacytic/eosinophilic nephritis. A 25 cm long worm was morphologically and molecularly analyzed, showing morphometric characteristics compatible with <em>D. renale.</em> Mitochondrial <em>cox</em>1 and ribosomal 18S and ITS1 sequences derived from the specimen were highly similar (&gt;98 %) to <em>D. renale.</em> Phylogenetic analysis of <em>cox</em>1 data clustered Costa Rican and Argentinian sequences irrespective of host and haplotype network revealed five geographically distributed haplotypes, with one shared between Argentina and Costa Rica. This study provides the first evidence of <em>D. renale</em> in Costa Rica, indicating local transmission risk. Considering the parasite's complex life cycle involving aquatic intermediate and paratenic hosts and the presence of susceptible wild carnivores in our country, environmental exposure represents a key risk factor. The findings highlight the need for a One Health approach integrating veterinary, public health, and environmental strategies to improve detection, prevention, and awareness of dioctophymosis in Central America.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101387"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance to acaricides in the cattle tick Rhipicephalus microplus (Acari: Ixodidae) from dairy farms in the Triângulo Mineiro region, Minas Gerais, Brazil","authors":"Vinicius da Silva Rodrigues ,&nbsp;Luis Fernando Santana ,&nbsp;Maria Clara Oliveira Sousa ,&nbsp;Sabrina França de Castro ,&nbsp;Pedro Henrique Ferreira ,&nbsp;Guilherme Augusto Ferreira ,&nbsp;Thiago Achcar Contarin ,&nbsp;Rodrigo da Costa Maia ,&nbsp;Guilherme Marcondes Klafke ,&nbsp;Matias Pablo Juan Szabó","doi":"10.1016/j.vprsr.2025.101366","DOIUrl":"10.1016/j.vprsr.2025.101366","url":null,"abstract":"<div><div><em>Rhipicephalus microplus</em> is a major ectoparasite of cattle in tropical and subtropical regions and poses a serious threat to animal welfare and productivity. Chemical acaricides remain the main method of control in Brazil. Nevertheless, resistance to these compounds has become increasingly widespread. Minas Gerais is the leading milk-producing state in Brazil, yet data on acaricide resistance in one of its key dairy-producing region, the Triângulo Mineiro, remain scarce. This study evaluated the resistance of <em>R. microplus</em> populations from 15 dairy farms from nine municipalities in the Triângulo Mineiro using the larval packet test (LPT) against five commonly used acaricide compounds: cypermethrin, chlorpyrifos, fipronil, ivermectin, and amitraz. All tick populations showed high resistance to cypermethrin. High resistance to chlorpyrifos and fipronil was observed in 80 % and 67 % of farms, respectively. Resistance to ivermectin and amitraz was more variable but still present in most populations. Resistance to at least two compounds was detected on all farms. The most frequently used products on surveyed farms were based on pyrethroids and organophosphates, including unapproved or off-label compounds. These findings indicate a critical need for strategic, evidence-based tick control programs in the region and regular resistance monitoring to guide the selection of effective acaricides. These results provide an updated overview of acaricide resistance an important dairy region of Brazil and highlight the importance of coordinated efforts to slow down resistance development.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101366"},"PeriodicalIF":1.4,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A proposed strategic control approach for Dirofilaria immitis in Galápagos","authors":"Carla Andreea Culda ,&nbsp;Diego Páez-Rosas ,&nbsp;Rommel Lenin Vinueza ,&nbsp;Renato León ,&nbsp;Andrei Daniel Mihalca","doi":"10.1016/j.vprsr.2025.101361","DOIUrl":"10.1016/j.vprsr.2025.101361","url":null,"abstract":"<div><div>A strategic approach is proposed for controlling the invasive heartworm <em>Dirofilaria immitis</em> across the Galápagos Islands, where endemic fauna lacks natural resistance. This parasite poses a potential threat to the fitness of native wildlife, particularly the Galápagos sea lions (<em>Zalophus wollebaeki</em>), due to its interaction with the multi-host parasite <em>D. immitis</em>, domestic dogs and suitable mosquito vectors. The control program is based on a detailed understanding of the local life cycle and ecoepidemiology of the parasite. An active surveillance program is essential for successful control and should include testing of resident dogs and cats, passive surveillance, and monitoring of mosquito populations. All infected domestic carnivorans must be treated according to American Heartworm Society (AHS) guidelines, and mosquito control is crucial for reducing the risk of transmission. Additionally, collaboration, technology transfer, and institutional articulation between local entities and academia are essential. These initiatives enhance scientific understanding, improve practical applications, benefit communities, and promote sustainable development.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101361"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative susceptibility of native chicken breeds of Bangladesh to experimental Eimeria tenella infection","authors":"Dipesh Aryal,&nbsp;Kazi Farah Tasfia,&nbsp;Al Nur Tarak,&nbsp;Asmita Bhujel,&nbsp;Md. Shahiduzzaman","doi":"10.1016/j.vprsr.2025.101353","DOIUrl":"10.1016/j.vprsr.2025.101353","url":null,"abstract":"<div><div><em>Eimeria tenella</em> is the major protozoan parasite that causes coccidiosis in chickens, and its clinical and pathological response shows significant variations among breeds. This disease has a major economic impact on growers and the poultry industry worldwide. Very little information exists concerning the challenge infections of native chicken breeds of Bangladesh. The present study was undertaken to investigate and compare the pathology and clinical manifestations of experimental <em>E. tenella</em> infection among native (indigenous) chicken breeds of Bangladesh (Hilly, Naked Neck, and Deshi), in comparison to the susceptible exotic Sonali breed. In order to achieve this, a controlled experimental infection was carried out, and weight changes, Feed Conversion ratio (FCR), OPG counts, lesion severity, qPCR quantification of oocysts output, and dropping score were measured at 6, 10, and 14-Days post-infection (DPI). Distinct breed-specific differences in susceptibility were elicited. Sonali chickens exhibited the highest oocyst counts, reaching up to 103,200 at 14 DPI, along with significant weight loss and severe cecal pathology marked by diffuse hemorrhagic lesions. On the other hand, the indigenous breeds had low OPG counts as low as 100 in Hilly chickens, insignificant changes in weights, and mild pathological features characterized by reduced inflammation and hemorrhagic enteritis. This study highlights significant breed-specific differences in susceptibility to <em>E. tenella</em>, offering valuable insights into potential resistance or tolerance mechanisms. These findings support the use of native breeds in selective breeding programs aimed at improving disease resistance and underscore the importance of targeted control strategies for effective coccidiosis prevention and management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101353"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors for Acanthocheilonema reconditum infection in dogs in the municipality of Lages, Santa Catarina, Brazil","authors":"Faiane Reila Sousa Centenaro Duarte ,&nbsp;Geanice Ledo ,&nbsp;Felipe Rieth de Lima ,&nbsp;Maria Laura Correa Nunes ,&nbsp;Julia Milena Sandri Pereira ,&nbsp;Hanna Christna Antonia Brasil ,&nbsp;Ana Paula Remor Sebolt ,&nbsp;Larissa Américo ,&nbsp;Lúcia Padilha Cury Thomaz de Aquino ,&nbsp;Anderson Barbosa de Moura ,&nbsp;Andreas Lazaros Chryssafidis","doi":"10.1016/j.vprsr.2025.101352","DOIUrl":"10.1016/j.vprsr.2025.101352","url":null,"abstract":"<div><div><em>Acanthocheilonema reconditum</em> (syn. <em>Dipetalonema reconditum</em>) is a filarial nematode transmitted by arthropods (fleas, lice, and ticks), primarily parasitizing the subcutaneous tissue of canids, but also found in perirenal fat. The clinical relevance of <em>A. reconditum</em> is often overshadowed by <em>Dirofilaria immitis</em>, a more pathogenic nematode with which it can be easily confused during diagnosis There are no reports of <em>D. immitis</em> in Lages, Santa Catarina (SC), neither in the Santa Catarina Plateau, Brazil. However, local laboratories observed microfilariae in canine blood samples and requested identification by the Veterinary Parasitology Laboratory of the State University of Santa Catarina. Morphological evaluation confirmed <em>A. reconditum</em> larvae. This study aimed to determine the prevalence of <em>A. reconditum</em> infection and assess risk factors in dogs from Lages. A total of 444 blood samples were collected from dogs in different neighborhoods, with simultaneous coat inspection for ectoparasites. Information on age, sex, origin, breed, ectoparasite control, and presence or absence of clinical signs was obtained through an epidemiological questionnaire to assess risk factors for <em>A. reconditum</em> infection. Blood samples were analyzed by blood smear, modified Knott's test, and immunochromatographic test. The prevalence of <em>A. reconditum</em> was 22.07 % (98/444) by the modified Knott's test, whereas the blood smear detected only 4.27 % (19/444). None of the evaluated samples showed characteristics compatible with <em>D. immitis</em> in the Knott's test, and all tested negative in the immunochromatographic test, indicating the absence of circulating antigen of this species. Ectoparasites were found in 35.58 % (158/444) of the animals, with no general association as a risk factor for <em>A. reconditum</em> infection. Among ectoparasites, Siphonaptera predominated, present in 93.02 % of parasitized animals, with a significant association between <em>Pulex irritans</em> presence (<em>p =</em> 0.003) and <em>A. reconditum</em> infection. This study confirmed the absence of <em>D. immitis</em> in Lages and highlighted a high prevalence of <em>A. reconditum</em>, underscoring the zoonotic potential and the need for further investigations.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101352"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}