F. Li, X. Yuan, L. Huang, Q. Liu, W. Chen, C. Wang
Deoxynivalenol (DON) is widely present in grain-based feeds and food. It has attracted great attention due to its high contamination rate and strong toxicity. The objective of this study was to analyse the toxic effects of DON on the liver and kidneys of weaned rabbits. 45 weaned male rabbits were allocated into control, low DON dose (0.5 mg/kg body weight), and high DON dose (1.5 mg/kg body weight) groups. Saline or DON was administrated intragastrically in the empty stomach of rabbits every morning. After 24 days of treatment, liver and kidney samples were collected for histological, reverse transcription-quantitative polymerase chain reaction (qRT-PCR), and immunohistochemistry analyses. Haematoxylin eosin staining showed that 0.5 mg/kg BW DON caused mild damage to the liver and kidney morphology, while 1.5 mg/kg body weight DON resulted in hepatic vacuolation and necrosis, as well as tubular stenosis and lesions. Data from qRT-PCR, Western blot, and immunohistochemistry revealed that the mRNA and protein expression and the distribution range of extracellular signal-regulated kinase, p38, and c-Jun NH2-terminal kinase were increased in the liver and kidneys. In conclusion, DON at the tested concentrations damaged the liver and kidneys of rabbits by affecting the expression of key proteins from the mitogen-activated protein kinase signalling pathway. The damage extent was proportional to the amount of DON ingested.
{"title":"Effects of deoxynivalenol on the histomorphology of the liver and kidneys and the expression of MAPKs in weaned rabbits","authors":"F. Li, X. Yuan, L. Huang, Q. Liu, W. Chen, C. Wang","doi":"10.3920/wmj2021.2753","DOIUrl":"https://doi.org/10.3920/wmj2021.2753","url":null,"abstract":"Deoxynivalenol (DON) is widely present in grain-based feeds and food. It has attracted great attention due to its high contamination rate and strong toxicity. The objective of this study was to analyse the toxic effects of DON on the liver and kidneys of weaned rabbits. 45 weaned male rabbits were allocated into control, low DON dose (0.5 mg/kg body weight), and high DON dose (1.5 mg/kg body weight) groups. Saline or DON was administrated intragastrically in the empty stomach of rabbits every morning. After 24 days of treatment, liver and kidney samples were collected for histological, reverse transcription-quantitative polymerase chain reaction (qRT-PCR), and immunohistochemistry analyses. Haematoxylin eosin staining showed that 0.5 mg/kg BW DON caused mild damage to the liver and kidney morphology, while 1.5 mg/kg body weight DON resulted in hepatic vacuolation and necrosis, as well as tubular stenosis and lesions. Data from qRT-PCR, Western blot, and immunohistochemistry revealed that the mRNA and protein expression and the distribution range of extracellular signal-regulated kinase, p38, and c-Jun NH2-terminal kinase were increased in the liver and kidneys. In conclusion, DON at the tested concentrations damaged the liver and kidneys of rabbits by affecting the expression of key proteins from the mitogen-activated protein kinase signalling pathway. The damage extent was proportional to the amount of DON ingested.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46393480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Kelman, J. Renaud, D. Baines, K. Yeung, J. Miller, M. Sumarah
Silage has become a key component of year-long animal feed in Canada and parts of northern Europe. It provides several advantages to farmers over traditional feed components, such as increased digestibility, higher nutrient content and preservation of the forages to meet seasonal feeding demands. Some ensiled materials can contain toxic fungal metabolites resulting from ‘in field’ contamination. In addition, when improperly stored or exposed to air during the feedout stage, silage is highly susceptible to aerobic spoilage by yeasts and filamentous fungi resulting in lower nutrient value and further mycotoxin contamination. In this study, silage samples were collected from 25 Canadian dairy goat and cattle farms where animals experienced feed-related health issues. Twenty-six unique fungal species were isolated from these samples, with the majority being Penicillium. High resolution liquid chromatography tandem mass spectrometry (HRLC-MS/MS) was used to identify a total of 125 known mycotoxins and fungal secondary metabolites from these silage samples, many of which were not produced by the 26 isolated filamentous fungi grown in agar cultures. Various mycotoxins resulting from preharvest contamination were detected, including ergot alkaloids, fumonisins and trichothecenes, some in high concentrations. Toxins produced after harvest included roquefortine C, citrinin and penitrem A. This study reinforces the need for farmers to implement best management practices to minimise fungal contamination and the resulting mycotoxin deposition in their crop and stored feed to maintain animal health.
{"title":"Mycotoxin determination in fungal contaminated Canadian silage toxic to dairy cows and goats","authors":"M. J. Kelman, J. Renaud, D. Baines, K. Yeung, J. Miller, M. Sumarah","doi":"10.3920/wmj2021.2764","DOIUrl":"https://doi.org/10.3920/wmj2021.2764","url":null,"abstract":"Silage has become a key component of year-long animal feed in Canada and parts of northern Europe. It provides several advantages to farmers over traditional feed components, such as increased digestibility, higher nutrient content and preservation of the forages to meet seasonal feeding demands. Some ensiled materials can contain toxic fungal metabolites resulting from ‘in field’ contamination. In addition, when improperly stored or exposed to air during the feedout stage, silage is highly susceptible to aerobic spoilage by yeasts and filamentous fungi resulting in lower nutrient value and further mycotoxin contamination. In this study, silage samples were collected from 25 Canadian dairy goat and cattle farms where animals experienced feed-related health issues. Twenty-six unique fungal species were isolated from these samples, with the majority being Penicillium. High resolution liquid chromatography tandem mass spectrometry (HRLC-MS/MS) was used to identify a total of 125 known mycotoxins and fungal secondary metabolites from these silage samples, many of which were not produced by the 26 isolated filamentous fungi grown in agar cultures. Various mycotoxins resulting from preharvest contamination were detected, including ergot alkaloids, fumonisins and trichothecenes, some in high concentrations. Toxins produced after harvest included roquefortine C, citrinin and penitrem A. This study reinforces the need for farmers to implement best management practices to minimise fungal contamination and the resulting mycotoxin deposition in their crop and stored feed to maintain animal health.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44150156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kagot, M. de Boevre, S. de Saeger, A. Moretti, M. Mwamuye, S. Okoth
Aspergillus and Fusarium are fungal genera that include toxigenic and pathogenic species, able to suffuse farmers’ crops and secrete an array of small molecular weight secondary metabolites which can cause health complications to humans and animals when ingested. In sub-Sahara Africa, contamination and persistence of these fungi is increased by the tropical climatic conditions which are ideal for the fungi to thrive. This study evaluated the incidence, regional distribution and toxigenic potential of Aspergillus and Fusarium species occurring in maize kernels from Eastern, Western, Coastal and the Lake Victoria agro-ecological zones of Kenya. Maize kernels were collected from 16 households in each agro-ecological zone. Single spore technique was used to isolate pure cultures of Aspergillus and Fusarium which were identified morphologically. Further, molecular analysis was done using the internal transcribed spacer 1 (ITS 1) region of the ribosomal DNA for Aspergillus and the translation elongation factor-1 alpha (TEF-1α) for Fusarium. The potential of the isolated fungi to produce mycotoxins was probed by polymerase chain reaction (PCR) based on the aflatoxin regulatory aflaR gene in Aspergillus, and the fumonisin backbone structure gene FUM1 in Fusarium. Among the potentially aflatoxigenic A. flavus species isolated, 55% were from Eastern, 27% from the Coastal zone, 13% from Lake Victoria zone and 5% from Western Kenya. Among the potentially fumonisin producing F. verticillioides isolated, 45% were from the Lake Victoria agro-ecological zone, 30% were from Western, 15% from Eastern Kenya and 10% from the Coastal agro-ecological zone. This study adds data on potential mycotoxin hotspots in Kenya useful in employing targeted and regional mycotoxin mitigation strategies in efforts to avert future mycotoxicoses outbreaks in Kenya.
{"title":"Incidence of toxigenic Aspergillus and Fusarium species occurring in maize kernels from Kenyan households","authors":"V. Kagot, M. de Boevre, S. de Saeger, A. Moretti, M. Mwamuye, S. Okoth","doi":"10.3920/wmj2021.2748","DOIUrl":"https://doi.org/10.3920/wmj2021.2748","url":null,"abstract":"Aspergillus and Fusarium are fungal genera that include toxigenic and pathogenic species, able to suffuse farmers’ crops and secrete an array of small molecular weight secondary metabolites which can cause health complications to humans and animals when ingested. In sub-Sahara Africa, contamination and persistence of these fungi is increased by the tropical climatic conditions which are ideal for the fungi to thrive. This study evaluated the incidence, regional distribution and toxigenic potential of Aspergillus and Fusarium species occurring in maize kernels from Eastern, Western, Coastal and the Lake Victoria agro-ecological zones of Kenya. Maize kernels were collected from 16 households in each agro-ecological zone. Single spore technique was used to isolate pure cultures of Aspergillus and Fusarium which were identified morphologically. Further, molecular analysis was done using the internal transcribed spacer 1 (ITS 1) region of the ribosomal DNA for Aspergillus and the translation elongation factor-1 alpha (TEF-1α) for Fusarium. The potential of the isolated fungi to produce mycotoxins was probed by polymerase chain reaction (PCR) based on the aflatoxin regulatory aflaR gene in Aspergillus, and the fumonisin backbone structure gene FUM1 in Fusarium. Among the potentially aflatoxigenic A. flavus species isolated, 55% were from Eastern, 27% from the Coastal zone, 13% from Lake Victoria zone and 5% from Western Kenya. Among the potentially fumonisin producing F. verticillioides isolated, 45% were from the Lake Victoria agro-ecological zone, 30% were from Western, 15% from Eastern Kenya and 10% from the Coastal agro-ecological zone. This study adds data on potential mycotoxin hotspots in Kenya useful in employing targeted and regional mycotoxin mitigation strategies in efforts to avert future mycotoxicoses outbreaks in Kenya.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45166872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ochratoxin A (OTA) is a possible human carcinogen commonly found in various agricultural commodities worldwide. While this potent nephrotoxin tends to survive common food processes and contaminate food products, certain process with higher temperature treatments may reduce OTA contents. Roasting has been suggested as a possible method to reduce OTA in coffee beans with up to 90% reduction, which may be applied to other food commodities. In this study, the possible influence of fibres on the reduction of OTA was investigated with brown and white rice with 2.2 and 6.7% of total dietary fibre content, respectively, roasting at two different temperatures (160 and 200 °C) for up to 30 min. The results showed that the rate and extent of OTA reduction were dependent on time, temperature, and rice type; greater than 60% of OTA reduction were achieved at 200 °C for 30 min for white rice and 37% for brown rice at the same conditions. No significant differences in reduction were observed between the samples roasted at 160 °C for 30 min and 200 °C for 15 min for both the white and brown rice, while white rice roasted at 160 °C for 15 min during roasting may be affected by the presence of fibre and/or fat in the cereal grains.
{"title":"Varied reduction of ochratoxin A in brown and white rice during roasting","authors":"H.N. Carbon, H. Lee","doi":"10.3920/wmj2021.2712","DOIUrl":"https://doi.org/10.3920/wmj2021.2712","url":null,"abstract":"Ochratoxin A (OTA) is a possible human carcinogen commonly found in various agricultural commodities worldwide. While this potent nephrotoxin tends to survive common food processes and contaminate food products, certain process with higher temperature treatments may reduce OTA contents. Roasting has been suggested as a possible method to reduce OTA in coffee beans with up to 90% reduction, which may be applied to other food commodities. In this study, the possible influence of fibres on the reduction of OTA was investigated with brown and white rice with 2.2 and 6.7% of total dietary fibre content, respectively, roasting at two different temperatures (160 and 200 °C) for up to 30 min. The results showed that the rate and extent of OTA reduction were dependent on time, temperature, and rice type; greater than 60% of OTA reduction were achieved at 200 °C for 30 min for white rice and 37% for brown rice at the same conditions. No significant differences in reduction were observed between the samples roasted at 160 °C for 30 min and 200 °C for 15 min for both the white and brown rice, while white rice roasted at 160 °C for 15 min during roasting may be affected by the presence of fibre and/or fat in the cereal grains.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43366882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Narváez, Y. Rodríguez-Carrasco, A. Ritieni, J. Mañes
Human biomonitoring (HBM) represents the most accurate approach for assessing the exposure to mycotoxins, but traditional matrices fail to provide information about long-term exposure due to the rapid excretion rates and short half-lives of mycotoxins. Hair emerges as a promising matrix considering that contaminants can form stable links with hair components, such as keratins and melanin. Hence, the aim of the present study was to monitor the presence of up to ten mycotoxins (aflatoxins and Fusarium mycotoxins) in human hair samples (n=100) through a high-performance liquid chromatography coupled to Q-TOF high resolution mass spectrometry. A prevalence of 43% at concentrations ranging from 2.7 to 106.1 ng/g was observed, being enniatins and aflatoxin B1 the most prevalent compounds. Co-occurrence of up to three mycotoxins was observed in 42% of the positive samples. Retrospective untargeted analysis of hair samples tentatively identified up to 128 mycotoxins and related metabolites. These results confirm the accumulation of toxicologically relevant mycotoxins in hair matrix, thus standing as a suitable matrix for assessing long-term exposure.
{"title":"Human biomonitoring of multiple mycotoxins in hair: first large-scale pilot study","authors":"A. Narváez, Y. Rodríguez-Carrasco, A. Ritieni, J. Mañes","doi":"10.3920/wmj2021.2744","DOIUrl":"https://doi.org/10.3920/wmj2021.2744","url":null,"abstract":"Human biomonitoring (HBM) represents the most accurate approach for assessing the exposure to mycotoxins, but traditional matrices fail to provide information about long-term exposure due to the rapid excretion rates and short half-lives of mycotoxins. Hair emerges as a promising matrix considering that contaminants can form stable links with hair components, such as keratins and melanin. Hence, the aim of the present study was to monitor the presence of up to ten mycotoxins (aflatoxins and Fusarium mycotoxins) in human hair samples (n=100) through a high-performance liquid chromatography coupled to Q-TOF high resolution mass spectrometry. A prevalence of 43% at concentrations ranging from 2.7 to 106.1 ng/g was observed, being enniatins and aflatoxin B1 the most prevalent compounds. Co-occurrence of up to three mycotoxins was observed in 42% of the positive samples. Retrospective untargeted analysis of hair samples tentatively identified up to 128 mycotoxins and related metabolites. These results confirm the accumulation of toxicologically relevant mycotoxins in hair matrix, thus standing as a suitable matrix for assessing long-term exposure.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47786206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S.A. Tittlemier, B. Cramer, C. Dall’Asta, M.C. DeRosa, V.M.T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M.W. Sumarah
This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.
{"title":"Developments in mycotoxin analysis: an update for 2020-2021","authors":"S.A. Tittlemier, B. Cramer, C. Dall’Asta, M.C. DeRosa, V.M.T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M.W. Sumarah","doi":"10.3920/wmj2021.2752","DOIUrl":"https://doi.org/10.3920/wmj2021.2752","url":null,"abstract":"This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":"2 s3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138495093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Ghafari, G. Paimard, E. Sadeghi, N. Choobkar, M. A. Lalabadi
In the present study, we report three methods of silica nanoparticles (SNPs) as adsorbent, ultraviolet (UV) irradiation, and microwave heating and evaluate their capabilities in reducing and eliminating zearalenone (ZEN). The offered method not only was used for ZEN detoxification, but also greatly enhanced the sensitivity of ZEN measurement. The aim of this study was to evaluate ZEN concentration in sunflower oil samples by high-performance liquid chromatography (HPLC) method. This method was successfully validated for sunflower oil samples while the limit of detection (LOD) method (signal-to-noise ratio of 3:1) was 0.5 μg/l. The acquired removal data with the HPLC method through SNPs were fitted well with Freundlich isotherm, denoting that the multi-layer adsorption took place on the adsorbent. The equilibrium adsorption capacity of ZEN was 61.02 μg/g in an optimum time of 240 min on SNPs. The experimental results were evaluated by the adsorption kinetic model, which specified the adsorption kinetics of ZEN on SNPs, obeying the pseudo-second order model. This model demonstrated that the sorption rate depended on the sorption capacity but not the concentration of the sorbate. Moreover, the method presented to determine ZEN based on the use of SNPs in sunflower oil was accomplished by the adsorption process. Furthermore, the removal efficiencies of ZEN by SNPs, UV irradiation, and microwave heating were compared and obtained to be 92.1, 96.22, and 37.30%, respectively for determined times. These results confirm the removal efficiency of these methods is sensitive enough to ZEN analysis in sunflower oil samples.
{"title":"Evaluation of nano-silica, microwave heating, and ultraviolet irradiation effects on zearalenone detoxification in sunflower oils","authors":"N. Ghafari, G. Paimard, E. Sadeghi, N. Choobkar, M. A. Lalabadi","doi":"10.3920/wmj2021.2733","DOIUrl":"https://doi.org/10.3920/wmj2021.2733","url":null,"abstract":"In the present study, we report three methods of silica nanoparticles (SNPs) as adsorbent, ultraviolet (UV) irradiation, and microwave heating and evaluate their capabilities in reducing and eliminating zearalenone (ZEN). The offered method not only was used for ZEN detoxification, but also greatly enhanced the sensitivity of ZEN measurement. The aim of this study was to evaluate ZEN concentration in sunflower oil samples by high-performance liquid chromatography (HPLC) method. This method was successfully validated for sunflower oil samples while the limit of detection (LOD) method (signal-to-noise ratio of 3:1) was 0.5 μg/l. The acquired removal data with the HPLC method through SNPs were fitted well with Freundlich isotherm, denoting that the multi-layer adsorption took place on the adsorbent. The equilibrium adsorption capacity of ZEN was 61.02 μg/g in an optimum time of 240 min on SNPs. The experimental results were evaluated by the adsorption kinetic model, which specified the adsorption kinetics of ZEN on SNPs, obeying the pseudo-second order model. This model demonstrated that the sorption rate depended on the sorption capacity but not the concentration of the sorbate. Moreover, the method presented to determine ZEN based on the use of SNPs in sunflower oil was accomplished by the adsorption process. Furthermore, the removal efficiencies of ZEN by SNPs, UV irradiation, and microwave heating were compared and obtained to be 92.1, 96.22, and 37.30%, respectively for determined times. These results confirm the removal efficiency of these methods is sensitive enough to ZEN analysis in sunflower oil samples.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":"4 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41301190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the Great Lakes region of North America, Gibberella ear rot (GER), caused by Fusarium graminearum, affects grain quality due to the accumulation of mycotoxins. GER severity is strongly influenced by environmental conditions; however, agronomic practices can also influence disease severity and mycotoxin accumulation. In this study, three separate small-plot experiments were conducted at Ridgetown, ON, Canada during 2019 and 2020 under an inoculated-misted system to determine Fusarium mycotoxin accumulation as affected by: (1) plant population density; (2) in-row-plant developmental variability; and (3) the effect of integrated Bt refuge genetics. In this study, DON concentrations were at least 49% higher in maize at 113,600 plants/ha compared to 79,000 plants/ha. Moreover, mycotoxin accumulation was higher in plants that were delayed developmentally in the crop row; total DON concentrations were at least 310% higher in late silked plants adjacent to early silked plants. Results of the plant population density and in-row-plant developmental variability suggest that the main driver for mycotoxin accumulation was stress induced by plant competition rather than environmental conditions; this highlights the importance of avoiding plant competitive stress as a strategy to reduce the risks of mycotoxin accumulation. In this study, there was no statistical difference in DON accumulation between the Bt component and the non-Bt component in each of the four hybrids tested; however, there was evidence that hybrids varied in susceptibility, including the Bt and non-Bt components that were paired commercially in a bag of seed maize. Reducing mycotoxins in maize requires integrated management, which includes agronomic considerations. These results indicate that mycotoxins are favoured with high plant populations and plant-to-plant variability in the row, especially in susceptible hybrids.
{"title":"Impact of agronomic practices on Fusarium mycotoxin accumulation in maize grain","authors":"K. Eli, A. Schaafsma, D. Hooker","doi":"10.3920/wmj2021.2734","DOIUrl":"https://doi.org/10.3920/wmj2021.2734","url":null,"abstract":"In the Great Lakes region of North America, Gibberella ear rot (GER), caused by Fusarium graminearum, affects grain quality due to the accumulation of mycotoxins. GER severity is strongly influenced by environmental conditions; however, agronomic practices can also influence disease severity and mycotoxin accumulation. In this study, three separate small-plot experiments were conducted at Ridgetown, ON, Canada during 2019 and 2020 under an inoculated-misted system to determine Fusarium mycotoxin accumulation as affected by: (1) plant population density; (2) in-row-plant developmental variability; and (3) the effect of integrated Bt refuge genetics. In this study, DON concentrations were at least 49% higher in maize at 113,600 plants/ha compared to 79,000 plants/ha. Moreover, mycotoxin accumulation was higher in plants that were delayed developmentally in the crop row; total DON concentrations were at least 310% higher in late silked plants adjacent to early silked plants. Results of the plant population density and in-row-plant developmental variability suggest that the main driver for mycotoxin accumulation was stress induced by plant competition rather than environmental conditions; this highlights the importance of avoiding plant competitive stress as a strategy to reduce the risks of mycotoxin accumulation. In this study, there was no statistical difference in DON accumulation between the Bt component and the non-Bt component in each of the four hybrids tested; however, there was evidence that hybrids varied in susceptibility, including the Bt and non-Bt components that were paired commercially in a bag of seed maize. Reducing mycotoxins in maize requires integrated management, which includes agronomic considerations. These results indicate that mycotoxins are favoured with high plant populations and plant-to-plant variability in the row, especially in susceptible hybrids.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42546748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F.G. Silva, L. Zanin, C. F. Shimizu, D. D. Lopes, J. C. Ribeiro, A. T. Ishikawa, E. N. Itano, O. Kawamura, E. Y. Hirooka
An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.
{"title":"Aflatoxin B1 in the egg chain: monitoring with specific indirect competitive ELISA in northern Paraná, Brazil","authors":"F.G. Silva, L. Zanin, C. F. Shimizu, D. D. Lopes, J. C. Ribeiro, A. T. Ishikawa, E. N. Itano, O. Kawamura, E. Y. Hirooka","doi":"10.3920/wmj2021.2728","DOIUrl":"https://doi.org/10.3920/wmj2021.2728","url":null,"abstract":"An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48191873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Hamad, H. A. El-Makarem, A. Elaziz, A. Amer, B. El-Nogoumy, S. Abou-Alella
The incidence of the mycotoxin ochratoxin A (OTA) in cheeses constitutes a significant economic and health concern for producers and consumers alike. Recently, detoxification approaches using food additives to counteract mycotoxins have been widely recommended in the food industry. This study aimed to quantify OTA levels in some Egyptian cheese types, and experimentally determine the detoxification effect of bentonite both in vitro and in vivo. The examined Roomy and Karish cheese showed higher OTA levels (4.138 and 3.399 μg/kg, respectively) than other cheeses. Calcium bentonite presented higher adsorption efficiency than sodium bentonite at all concentrations, both in phosphate buffered saline (PBS) and feta cheese, and at the whole pH range. Calcium bentonite concentrations (60 and 100 mg/ml) had much higher sequestering activity on OTA both in PBS and feta cheese, while the adsorption efficiency was higher at pH 6.8 than at pH 3. All enzymatic activities were near the control levels in rats treated both with OTA and bentonite compared with rats treated with OTA alone. The IC50 of calcium bentonite was 107.75 μg/ml, which was less cytotoxic than sodium bentonite (52.96 μg/ml). Bentonites were categorised by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) as excellent binders of OTA. The innovative calcium bentonite-fortified feta cheese showed the most superior sensorial properties; hence it can be predicted as a novel food-grade adsorbent for OTA sequestration.
{"title":"Adsorption efficiency of sodium & calcium bentonite for ochratoxin A in some Egyptian cheeses: an innovative fortification model, in vitro and in vivo experiments","authors":"G. Hamad, H. A. El-Makarem, A. Elaziz, A. Amer, B. El-Nogoumy, S. Abou-Alella","doi":"10.3920/wmj2021.2682","DOIUrl":"https://doi.org/10.3920/wmj2021.2682","url":null,"abstract":"The incidence of the mycotoxin ochratoxin A (OTA) in cheeses constitutes a significant economic and health concern for producers and consumers alike. Recently, detoxification approaches using food additives to counteract mycotoxins have been widely recommended in the food industry. This study aimed to quantify OTA levels in some Egyptian cheese types, and experimentally determine the detoxification effect of bentonite both in vitro and in vivo. The examined Roomy and Karish cheese showed higher OTA levels (4.138 and 3.399 μg/kg, respectively) than other cheeses. Calcium bentonite presented higher adsorption efficiency than sodium bentonite at all concentrations, both in phosphate buffered saline (PBS) and feta cheese, and at the whole pH range. Calcium bentonite concentrations (60 and 100 mg/ml) had much higher sequestering activity on OTA both in PBS and feta cheese, while the adsorption efficiency was higher at pH 6.8 than at pH 3. All enzymatic activities were near the control levels in rats treated both with OTA and bentonite compared with rats treated with OTA alone. The IC50 of calcium bentonite was 107.75 μg/ml, which was less cytotoxic than sodium bentonite (52.96 μg/ml). Bentonites were categorised by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) as excellent binders of OTA. The innovative calcium bentonite-fortified feta cheese showed the most superior sensorial properties; hence it can be predicted as a novel food-grade adsorbent for OTA sequestration.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42284573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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