A. Cimbalo, M. Frangiamone, M. Lozano, L. Escrivá, P. Vila-Donat, L. Manyes
The beneficial effect of fermented whey and pumpkin extract rich in carotenoids was evaluated in Jurkat cells against aflatoxin B1 (AFB1) and ochratoxin A (OTA) cytotoxicity through a proteomic approach. The functional ingredients were added into mycotoxin contaminated bread formulation, which were digested in vitro in order to simulate human intestinal absorption. Cell cultures were exposed during 7 days to these mycotoxins dissolved in: (a) 0.1% organic solvent (DMSO), (b) an intestinal digest of bread with pumpkin individually (PID) and (c) an intestinal digest of bread with pumpkin mixed with fermented whey (PID+WF). Extracted proteins were subjected to reduction and alkylation and subsequently a tryptic digestion in order to be analysed by liquid chromatography coupled with quadrupole time of flight (LC/MS-Q-TOF). Results obtained highlighted the beneficial role of functional ingredients employed through the identification of proteins involved in several biological processes and metabolic pathways, mainly antioxidant activity, nucleosome assembly and secretory senescence phenotype. Among proteins involved in antioxidant activity, peroxiredoxin 1 and 2 stand out. Comparing the different conditions investigated, a remarkable change was observed in their expression, ranging from a repression using the standard (DMSO 0.1%), to an overexpression when treated with the functional ingredients. Similarly, after PID and PID+WF treatment, histones’ expression implicated in the metabolic pathway of nucleosome assembly, such as H2A, H2B, H2C, H3 and H4, was increased. Furthermore, the expression of protein cyclin A2, which downregulation is involved in limiting carcinogenic cells growth, was lower in presence of both functional ingredients. Based on these findings, functional ingredients can act as protectors against genomic stress caused by mycotoxins, preventing the loss of vital cell functions and paralysing the growth of carcinogenic cells.
{"title":"Protective role of fermented whey and pumpkin extract against aflatoxin B1 and ochratoxin A toxicity in Jurkat T-cells","authors":"A. Cimbalo, M. Frangiamone, M. Lozano, L. Escrivá, P. Vila-Donat, L. Manyes","doi":"10.3920/wmj2022.2780","DOIUrl":"https://doi.org/10.3920/wmj2022.2780","url":null,"abstract":"The beneficial effect of fermented whey and pumpkin extract rich in carotenoids was evaluated in Jurkat cells against aflatoxin B1 (AFB1) and ochratoxin A (OTA) cytotoxicity through a proteomic approach. The functional ingredients were added into mycotoxin contaminated bread formulation, which were digested in vitro in order to simulate human intestinal absorption. Cell cultures were exposed during 7 days to these mycotoxins dissolved in: (a) 0.1% organic solvent (DMSO), (b) an intestinal digest of bread with pumpkin individually (PID) and (c) an intestinal digest of bread with pumpkin mixed with fermented whey (PID+WF). Extracted proteins were subjected to reduction and alkylation and subsequently a tryptic digestion in order to be analysed by liquid chromatography coupled with quadrupole time of flight (LC/MS-Q-TOF). Results obtained highlighted the beneficial role of functional ingredients employed through the identification of proteins involved in several biological processes and metabolic pathways, mainly antioxidant activity, nucleosome assembly and secretory senescence phenotype. Among proteins involved in antioxidant activity, peroxiredoxin 1 and 2 stand out. Comparing the different conditions investigated, a remarkable change was observed in their expression, ranging from a repression using the standard (DMSO 0.1%), to an overexpression when treated with the functional ingredients. Similarly, after PID and PID+WF treatment, histones’ expression implicated in the metabolic pathway of nucleosome assembly, such as H2A, H2B, H2C, H3 and H4, was increased. Furthermore, the expression of protein cyclin A2, which downregulation is involved in limiting carcinogenic cells growth, was lower in presence of both functional ingredients. Based on these findings, functional ingredients can act as protectors against genomic stress caused by mycotoxins, preventing the loss of vital cell functions and paralysing the growth of carcinogenic cells.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48260209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Mahuku, A. Mauro, B. Pallangyo, E. Nsami, S. Boni, E. Koyano, O. Mponda, A. Ortega‐Beltran, J. Atehnkeng, F. Aquiline, R. Samuel, J. Njela, P. Cotty, R. Bandyopadhyay
Application of biocontrol products containing atoxigenic isolates of Aspergillus flavus to reduce aflatoxin content in crops is an effective strategy for managing aflatoxin in several regions throughout the world. We report the development and validation of two aflatoxin biocontrol products, Aflasafe TZ01 and Aflasafe TZ02, for use in maize and groundnut in Tanzania, a country frequently affected by aflatoxin contamination. Each product contains four atoxigenic A. flavus genotypes native and widely distributed in Tanzania. Efficacy tests on maize and groundnut were conducted over two years and in four regions of Tanzania where aflatoxin contamination is prevalent. Application of both products significantly (P<0.05) reduced aflatoxin levels in maize and groundnut in both years and in all districts. No differences were observed in total Aspergillus section Flavi population in treated and untreated fields, revealing that application of the biocontrol products do not alter overall Aspergillus populations in the environment. The results indicate that both products are effective tools for aflatoxin mitigation in groundnut and maize. The products were officially registered in 2018. Currently, there are scale-out and-up efforts of aflatoxin biocontrol products in Tanzania through a private sector company that is making the products available to farmers. Protecting maize and groundnut from aflatoxin contamination in Tanzania can result in health, income, and trade benefits.
{"title":"Atoxigenic-based technology for biocontrol of aflatoxin in maize and groundnuts for Tanzania","authors":"G. Mahuku, A. Mauro, B. Pallangyo, E. Nsami, S. Boni, E. Koyano, O. Mponda, A. Ortega‐Beltran, J. Atehnkeng, F. Aquiline, R. Samuel, J. Njela, P. Cotty, R. Bandyopadhyay","doi":"10.3920/wmj2021.2758","DOIUrl":"https://doi.org/10.3920/wmj2021.2758","url":null,"abstract":"Application of biocontrol products containing atoxigenic isolates of Aspergillus flavus to reduce aflatoxin content in crops is an effective strategy for managing aflatoxin in several regions throughout the world. We report the development and validation of two aflatoxin biocontrol products, Aflasafe TZ01 and Aflasafe TZ02, for use in maize and groundnut in Tanzania, a country frequently affected by aflatoxin contamination. Each product contains four atoxigenic A. flavus genotypes native and widely distributed in Tanzania. Efficacy tests on maize and groundnut were conducted over two years and in four regions of Tanzania where aflatoxin contamination is prevalent. Application of both products significantly (P<0.05) reduced aflatoxin levels in maize and groundnut in both years and in all districts. No differences were observed in total Aspergillus section Flavi population in treated and untreated fields, revealing that application of the biocontrol products do not alter overall Aspergillus populations in the environment. The results indicate that both products are effective tools for aflatoxin mitigation in groundnut and maize. The products were officially registered in 2018. Currently, there are scale-out and-up efforts of aflatoxin biocontrol products in Tanzania through a private sector company that is making the products available to farmers. Protecting maize and groundnut from aflatoxin contamination in Tanzania can result in health, income, and trade benefits.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48588212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aoun, C. Siegel, G. Windham, W. Williams, R. Nelson
Spectroscopy is a rapid, non-destructive, and low-cost analytical technique that has the potential to complement more resource-intensive analytical methods. We explored the use of spectral methods to differentiate maize genotypes and assess aflatoxin (AF) contamination in maize kernels. We compared the performance of two instruments: a research-grade ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer that measures reflectance from 304 -1,085 nm, and a miniaturised NIR spectrometer that measures reflectance from 740-1,070 nm. Both systems were used to predict AF levels in maize kernels from a single genotype and across 10 genotypes, and to predict genotype for the latter. A partial least square discriminant analysis model was trained on 70% of the kernels and tested on the remaining 30%. The classification accuracy for 10 maize genotypes was 71-72% using the UV-Vis-NIR instrument on 1,170 kernels, and 65-66% using the NIR device on 740 kernels. The classification accuracy for 247 AF-contaminated kernels of a single genotype using the UV-Vis-NIR instrument was 71, 82, and 92% for AF thresholds of 20, 100, and 1000 μg/kg, respectively. Using the same spectrometer on 872 kernels from 10 genotypes, AF classification accuracy was 67, 90, and 95% in validation sets for AF thresholds of 20, 100, and 1000 μg/kg, respectively. The UV-Vis-NIR instrument and the NIR device had similar classification accuracies for AF thresholds of 100 and 1000 μg/kg, whereas the NIR device had higher accuracy for the AF threshold of 20 μg/kg. Reflectance spectroscopy outperformed visual sorting and the bright greenish yellow fluorescence test in identifying AF levels. Applying spectral analysis to estimate mycotoxin levels and to identify maize genotypes could contribute to regional toxin surveillance and action efforts. Further, using AF-associated spectral features for grain sorting can reduce AF exposure.
{"title":"Application of reflectance spectroscopy to identify maize genotypes and aflatoxin levels in single kernels","authors":"M. Aoun, C. Siegel, G. Windham, W. Williams, R. Nelson","doi":"10.3920/wmj2021.2750","DOIUrl":"https://doi.org/10.3920/wmj2021.2750","url":null,"abstract":"Spectroscopy is a rapid, non-destructive, and low-cost analytical technique that has the potential to complement more resource-intensive analytical methods. We explored the use of spectral methods to differentiate maize genotypes and assess aflatoxin (AF) contamination in maize kernels. We compared the performance of two instruments: a research-grade ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer that measures reflectance from 304 -1,085 nm, and a miniaturised NIR spectrometer that measures reflectance from 740-1,070 nm. Both systems were used to predict AF levels in maize kernels from a single genotype and across 10 genotypes, and to predict genotype for the latter. A partial least square discriminant analysis model was trained on 70% of the kernels and tested on the remaining 30%. The classification accuracy for 10 maize genotypes was 71-72% using the UV-Vis-NIR instrument on 1,170 kernels, and 65-66% using the NIR device on 740 kernels. The classification accuracy for 247 AF-contaminated kernels of a single genotype using the UV-Vis-NIR instrument was 71, 82, and 92% for AF thresholds of 20, 100, and 1000 μg/kg, respectively. Using the same spectrometer on 872 kernels from 10 genotypes, AF classification accuracy was 67, 90, and 95% in validation sets for AF thresholds of 20, 100, and 1000 μg/kg, respectively. The UV-Vis-NIR instrument and the NIR device had similar classification accuracies for AF thresholds of 100 and 1000 μg/kg, whereas the NIR device had higher accuracy for the AF threshold of 20 μg/kg. Reflectance spectroscopy outperformed visual sorting and the bright greenish yellow fluorescence test in identifying AF levels. Applying spectral analysis to estimate mycotoxin levels and to identify maize genotypes could contribute to regional toxin surveillance and action efforts. Further, using AF-associated spectral features for grain sorting can reduce AF exposure.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43924094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Tyska, A. Mallmann, J. Vidal, C. Simões, C. Mallmann
Mycotoxicological and nutritional analyses were conducted in maize from Argentina, Bolivia, Brazil (stratified by region), Colombia and Peru through near-infrared spectroscopy (NIRS) in 2020 and 2021. Concentrations of aflatoxin B1 (AFB1), fumonisins B1 + B2 (FBs), zearalenone (ZEN) and deoxynivalenol, in addition to water activity, crude protein (CP), ether extract (EE), starch and apparent metabolizable energy (AME) in poultry, were determined in 18,363 spectra (totalling 195,486 analyses). FBs were the most prevalent metabolites in South American maize, being detected in 91.6 and 91.9% of the samples; ZEN had the second highest positivity, 15.4 and 26.8%, followed by AFB1, 15.3 and 14.6% (2020 and 2021, respectively). FBs also had the highest incidence in Brazilian maize, 92.6 and 92.1%, followed by AFB1, 15.2 and 13.5%, and ZEN, 14.7 and 27.7% (2020 and 2021, respectively). Contamination with at least one mycotoxin was detected in 93.5% of the samples. The mycotoxins found co-contaminating the maize belong to the genera Fusarium and Aspergillus; FBs, followed by ZEN and AFB1, were the most prevalent toxins in the associations. Levels of water activity were below 0.70. Regarding chemical composition, CP presented the largest oscillation in relation to the mean in South American maize (both years). As for the Brazilian samples, the Southeast region showed the greatest positive variability in relation to the means for all evaluated parameters, followed by the Northeast region (excepting CP) in 2020; in 2021, the Central-West and Northeast regions had the highest levels of EE and AME. In both years, the South region presented lower levels in relation to the mean for most parameters. Data on prevalence, mycotoxicological contamination, storage conditions and nutritional quality of maize assist the decision-making process of raw material use. In this setting, NIRS suits the needs of the industry for providing information on multiple parameters in real time.
{"title":"Near-infrared spectroscopy to assess mycotoxins contamination and nutritional composition of maize marketed in South America, years 2020-2021","authors":"D. Tyska, A. Mallmann, J. Vidal, C. Simões, C. Mallmann","doi":"10.3920/wmj2022.2774","DOIUrl":"https://doi.org/10.3920/wmj2022.2774","url":null,"abstract":"Mycotoxicological and nutritional analyses were conducted in maize from Argentina, Bolivia, Brazil (stratified by region), Colombia and Peru through near-infrared spectroscopy (NIRS) in 2020 and 2021. Concentrations of aflatoxin B1 (AFB1), fumonisins B1 + B2 (FBs), zearalenone (ZEN) and deoxynivalenol, in addition to water activity, crude protein (CP), ether extract (EE), starch and apparent metabolizable energy (AME) in poultry, were determined in 18,363 spectra (totalling 195,486 analyses). FBs were the most prevalent metabolites in South American maize, being detected in 91.6 and 91.9% of the samples; ZEN had the second highest positivity, 15.4 and 26.8%, followed by AFB1, 15.3 and 14.6% (2020 and 2021, respectively). FBs also had the highest incidence in Brazilian maize, 92.6 and 92.1%, followed by AFB1, 15.2 and 13.5%, and ZEN, 14.7 and 27.7% (2020 and 2021, respectively). Contamination with at least one mycotoxin was detected in 93.5% of the samples. The mycotoxins found co-contaminating the maize belong to the genera Fusarium and Aspergillus; FBs, followed by ZEN and AFB1, were the most prevalent toxins in the associations. Levels of water activity were below 0.70. Regarding chemical composition, CP presented the largest oscillation in relation to the mean in South American maize (both years). As for the Brazilian samples, the Southeast region showed the greatest positive variability in relation to the means for all evaluated parameters, followed by the Northeast region (excepting CP) in 2020; in 2021, the Central-West and Northeast regions had the highest levels of EE and AME. In both years, the South region presented lower levels in relation to the mean for most parameters. Data on prevalence, mycotoxicological contamination, storage conditions and nutritional quality of maize assist the decision-making process of raw material use. In this setting, NIRS suits the needs of the industry for providing information on multiple parameters in real time.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46321523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aasa, F. Fru, O. A. Adelusi, S. Oyeyinka, P. Njobeh
Fungal contamination is a threat to food safety in West Africa with implications for food and feed due to their climate, which is characterised by high temperatures and high relative humidity, which are environmental favourable for fast fungal growth and mycotoxin production. This report gives perspective on studies on toxigenic fungi (Aspergillus, Fusarium and Penicillium) and their toxins, mainly aflatoxins, fumonisins and ochratoxins commonly found in some West African countries, including Benin, Burkina Faso, Gambia, Ghana, Ivory Coast, Mali, Nigeria, Senegal, Sierra Leone, and Togo. Only four of these countries have mycotoxins regulations in place for feeds and food products (Ghana, Ivory Coast, Nigeria, and Senegal). Food commodities that are widely consumed and were thoroughly investigated in this region include cereals, peanuts, cassava chips (flakes), cassava flour, chilies, peanuts, locust beans, melon, and yam products. In conclusion, authorities and scientists needed to consider research and approaches to monitor mycotoxins in foods and feeds produced and consumed in West Africa.
{"title":"A review of toxigenic fungi and mycotoxins in feeds and food commodities in West Africa","authors":"A. Aasa, F. Fru, O. A. Adelusi, S. Oyeyinka, P. Njobeh","doi":"10.3920/wmj2021.2766","DOIUrl":"https://doi.org/10.3920/wmj2021.2766","url":null,"abstract":"Fungal contamination is a threat to food safety in West Africa with implications for food and feed due to their climate, which is characterised by high temperatures and high relative humidity, which are environmental favourable for fast fungal growth and mycotoxin production. This report gives perspective on studies on toxigenic fungi (Aspergillus, Fusarium and Penicillium) and their toxins, mainly aflatoxins, fumonisins and ochratoxins commonly found in some West African countries, including Benin, Burkina Faso, Gambia, Ghana, Ivory Coast, Mali, Nigeria, Senegal, Sierra Leone, and Togo. Only four of these countries have mycotoxins regulations in place for feeds and food products (Ghana, Ivory Coast, Nigeria, and Senegal). Food commodities that are widely consumed and were thoroughly investigated in this region include cereals, peanuts, cassava chips (flakes), cassava flour, chilies, peanuts, locust beans, melon, and yam products. In conclusion, authorities and scientists needed to consider research and approaches to monitor mycotoxins in foods and feeds produced and consumed in West Africa.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48864602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Truong, K. Tesfamariam, L. Visintin, T. Goessens, S. de Saeger, C. Lachat, M. de Boevre
Mycotoxin contamination is a global challenge to food safety and population health. A diversity of adverse effects in human health such as organ damage, immunity disorders and carcinogenesis are attributed to acute and chronic exposure to mycotoxins. While there is a high likelihood of mycotoxin co-occurrence in the daily diet, multiple mycotoxin exposures represent a considerable challenge in understanding the accumulative effects of groups of exposures on health outcomes. Nevertheless, previous studies on mycotoxin exposure-health outcome associations have focused on a single or a limited number of exposures. To guide multi-exposure assessment, careful considerations of statistical approaches available are required. In addition, the issue of multicollinearity in high-dimensional settings of multiple exposure analysis underlies the controversy surrounding the reliability and consistency of statistical conclusions about the exposure-health outcome associations. Conventional approaches such as generalised linear regressions (GLR) in conjunction with regularisation methods, including ridge regression, lasso and elastic net, offer some clear advantages in terms of results’ interpretation and model selection. However, when highly-correlated variables are observed, these methods have shown a low specificity in variable selection. Principal component analysis (PCA) that has been widely used as a dimensionality reduction technique also has the limitation to identify important predictor variables as this approach may overlook the associations between certain components and health outcomes. Recently, some alternative approaches have been introduced to address the issues of high dimensionality and highly-correlated data in the context of epidemiological and environmental research. Two of the noticeable approaches are weighted quantile sum regression (WQSR) and Bayesian kernel machine regression (BKMR). Combining different methods of inference allows us to interpret the role of certain exposures, their interactions and the combined effects on human health under diverse statistical perspectives, which ultimately facilitate the construction of the toxicological profile of multiple mycotoxins’ exposure.
{"title":"Associating multiple mycotoxin exposure and health outcomes: current statistical approaches and challenges","authors":"N. Truong, K. Tesfamariam, L. Visintin, T. Goessens, S. de Saeger, C. Lachat, M. de Boevre","doi":"10.3920/wmj2022.2784","DOIUrl":"https://doi.org/10.3920/wmj2022.2784","url":null,"abstract":"Mycotoxin contamination is a global challenge to food safety and population health. A diversity of adverse effects in human health such as organ damage, immunity disorders and carcinogenesis are attributed to acute and chronic exposure to mycotoxins. While there is a high likelihood of mycotoxin co-occurrence in the daily diet, multiple mycotoxin exposures represent a considerable challenge in understanding the accumulative effects of groups of exposures on health outcomes. Nevertheless, previous studies on mycotoxin exposure-health outcome associations have focused on a single or a limited number of exposures. To guide multi-exposure assessment, careful considerations of statistical approaches available are required. In addition, the issue of multicollinearity in high-dimensional settings of multiple exposure analysis underlies the controversy surrounding the reliability and consistency of statistical conclusions about the exposure-health outcome associations. Conventional approaches such as generalised linear regressions (GLR) in conjunction with regularisation methods, including ridge regression, lasso and elastic net, offer some clear advantages in terms of results’ interpretation and model selection. However, when highly-correlated variables are observed, these methods have shown a low specificity in variable selection. Principal component analysis (PCA) that has been widely used as a dimensionality reduction technique also has the limitation to identify important predictor variables as this approach may overlook the associations between certain components and health outcomes. Recently, some alternative approaches have been introduced to address the issues of high dimensionality and highly-correlated data in the context of epidemiological and environmental research. Two of the noticeable approaches are weighted quantile sum regression (WQSR) and Bayesian kernel machine regression (BKMR). Combining different methods of inference allows us to interpret the role of certain exposures, their interactions and the combined effects on human health under diverse statistical perspectives, which ultimately facilitate the construction of the toxicological profile of multiple mycotoxins’ exposure.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42498843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deoxynivalenol (DON) is a toxic secondary metabolite in wheat which affects animal performance. Limited post-harvest sorting technologies are available to remove infected kernels thereby allowing safe use in livestock. A technology was developed which uses near-infrared spectrometry combined with a seed singulation sorter by BoMill AB (Sweden) which is purported by the manufacturer to remove Fusarium infected grain. The objective of this study was to determine if Fusarium infected grain could be removed using the BoMill equipped with the Fusarium calibration resulting in grain with less than 5,000 μg/kg DON, and therefore legal to feed to poultry and beef in Canada. The secondary objective was to determine the optimal HVK settings within the two calibrations to determine if sorting based on Fusarium damage is more effective than sorting based on protein content. The settings tested were HVK, HHVK, and HHHVK. The HVK settings are reported by the manufacturer to be related to the relative opacity from the starch granules. Using the HHVK setting in the Fusarium calibration resulted in highest recovery (50.3% vs HVK 40.8% and HHHVK 45.1%) and intermediate levels of DON (1,800 μg/kg vs HVK 1,600 μg/kg and HHHVK 2,400 μg/kg), and intermediate rejection rates (29.0% vs HVK 38.7% and HHHVK 22.7%). When using the protein calibration with HHVK setting, the recoveries were similar to the Fusarium calibration (51%), the rejection rates were lower (17.5%), but DON concentration was higher (2,900 μg/kg). Sorting of pooled samples was effective, however additional sieving was required to separate grain of like sizes for optimal function. BoMill sorting using the Fusarium calibration and HHVK setting will effectively sort high DON wheat. The Fusarium calibration was superior to the protein calibration as it resulted in similar recovery but lower DON concentrations.
脱氧雪腐镰刀菌烯醇（DON）是小麦中一种有毒的次生代谢产物，影响动物的生产性能。有限的收获后分拣技术可用于去除受感染的谷粒，从而允许在牲畜中安全使用。BoMill AB（瑞典）开发了一种技术，该技术使用近红外光谱法与种子分选机相结合，据称制造商可以去除镰刀菌感染的谷物。本研究的目的是确定是否可以使用配备镰刀菌校准的BoMill去除镰刀菌感染的谷物，从而使谷物DON低于5000μg/kg，因此在加拿大饲养家禽和牛肉是合法的。次要目标是在两次校准中确定最佳HVK设置，以确定基于镰刀菌损伤的分选是否比基于蛋白质含量的分选更有效。测试的设置为HVK、HHVK和HHHVK。制造商报告HVK设置与淀粉颗粒的相对不透明度有关。在镰刀菌校准中使用HHVK设置导致最高的回收率（50.3%vs HVK 40.8%和HHHVK 45.1%）和中等水平的DON（1800μg/kg vs HVK 1600μg/kg和HHHVK2400μg/kg），以及中等排斥率（29.0%vs HVK 38.7%和HHHVK22.7%）。当使用HHVK设置的蛋白质校准时，回收率与镰刀菌校正相似（51%），排异率较低（17.5%），DON浓度较高（2900μg/kg）。合并样品的分选是有效的，但需要额外的筛分来分离相同尺寸的颗粒以获得最佳功能。使用镰刀菌校准和HHVK设置的BoMill分选将有效地分选高DON小麦。镰刀菌校准优于蛋白质校准，因为它导致相似的回收率，但DON浓度较低。
{"title":"Sorting capability and grain recovery of deoxynivalenol contaminated wheat is affected by calibration and vitreous kernel settings from near-infrared transmittance technology","authors":"M. Taylor, R. Newkirk","doi":"10.3920/wmj2021.2751","DOIUrl":"https://doi.org/10.3920/wmj2021.2751","url":null,"abstract":"Deoxynivalenol (DON) is a toxic secondary metabolite in wheat which affects animal performance. Limited post-harvest sorting technologies are available to remove infected kernels thereby allowing safe use in livestock. A technology was developed which uses near-infrared spectrometry combined with a seed singulation sorter by BoMill AB (Sweden) which is purported by the manufacturer to remove Fusarium infected grain. The objective of this study was to determine if Fusarium infected grain could be removed using the BoMill equipped with the Fusarium calibration resulting in grain with less than 5,000 μg/kg DON, and therefore legal to feed to poultry and beef in Canada. The secondary objective was to determine the optimal HVK settings within the two calibrations to determine if sorting based on Fusarium damage is more effective than sorting based on protein content. The settings tested were HVK, HHVK, and HHHVK. The HVK settings are reported by the manufacturer to be related to the relative opacity from the starch granules. Using the HHVK setting in the Fusarium calibration resulted in highest recovery (50.3% vs HVK 40.8% and HHHVK 45.1%) and intermediate levels of DON (1,800 μg/kg vs HVK 1,600 μg/kg and HHHVK 2,400 μg/kg), and intermediate rejection rates (29.0% vs HVK 38.7% and HHHVK 22.7%). When using the protein calibration with HHVK setting, the recoveries were similar to the Fusarium calibration (51%), the rejection rates were lower (17.5%), but DON concentration was higher (2,900 μg/kg). Sorting of pooled samples was effective, however additional sieving was required to separate grain of like sizes for optimal function. BoMill sorting using the Fusarium calibration and HHVK setting will effectively sort high DON wheat. The Fusarium calibration was superior to the protein calibration as it resulted in similar recovery but lower DON concentrations.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46665015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Li, X. Yuan, L. Huang, Q. Liu, W. Chen, C. Wang
Deoxynivalenol (DON) is widely present in grain-based feeds and food. It has attracted great attention due to its high contamination rate and strong toxicity. The objective of this study was to analyse the toxic effects of DON on the liver and kidneys of weaned rabbits. 45 weaned male rabbits were allocated into control, low DON dose (0.5 mg/kg body weight), and high DON dose (1.5 mg/kg body weight) groups. Saline or DON was administrated intragastrically in the empty stomach of rabbits every morning. After 24 days of treatment, liver and kidney samples were collected for histological, reverse transcription-quantitative polymerase chain reaction (qRT-PCR), and immunohistochemistry analyses. Haematoxylin eosin staining showed that 0.5 mg/kg BW DON caused mild damage to the liver and kidney morphology, while 1.5 mg/kg body weight DON resulted in hepatic vacuolation and necrosis, as well as tubular stenosis and lesions. Data from qRT-PCR, Western blot, and immunohistochemistry revealed that the mRNA and protein expression and the distribution range of extracellular signal-regulated kinase, p38, and c-Jun NH2-terminal kinase were increased in the liver and kidneys. In conclusion, DON at the tested concentrations damaged the liver and kidneys of rabbits by affecting the expression of key proteins from the mitogen-activated protein kinase signalling pathway. The damage extent was proportional to the amount of DON ingested.
{"title":"Effects of deoxynivalenol on the histomorphology of the liver and kidneys and the expression of MAPKs in weaned rabbits","authors":"F. Li, X. Yuan, L. Huang, Q. Liu, W. Chen, C. Wang","doi":"10.3920/wmj2021.2753","DOIUrl":"https://doi.org/10.3920/wmj2021.2753","url":null,"abstract":"Deoxynivalenol (DON) is widely present in grain-based feeds and food. It has attracted great attention due to its high contamination rate and strong toxicity. The objective of this study was to analyse the toxic effects of DON on the liver and kidneys of weaned rabbits. 45 weaned male rabbits were allocated into control, low DON dose (0.5 mg/kg body weight), and high DON dose (1.5 mg/kg body weight) groups. Saline or DON was administrated intragastrically in the empty stomach of rabbits every morning. After 24 days of treatment, liver and kidney samples were collected for histological, reverse transcription-quantitative polymerase chain reaction (qRT-PCR), and immunohistochemistry analyses. Haematoxylin eosin staining showed that 0.5 mg/kg BW DON caused mild damage to the liver and kidney morphology, while 1.5 mg/kg body weight DON resulted in hepatic vacuolation and necrosis, as well as tubular stenosis and lesions. Data from qRT-PCR, Western blot, and immunohistochemistry revealed that the mRNA and protein expression and the distribution range of extracellular signal-regulated kinase, p38, and c-Jun NH2-terminal kinase were increased in the liver and kidneys. In conclusion, DON at the tested concentrations damaged the liver and kidneys of rabbits by affecting the expression of key proteins from the mitogen-activated protein kinase signalling pathway. The damage extent was proportional to the amount of DON ingested.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46393480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Kelman, J. Renaud, D. Baines, K. Yeung, J. Miller, M. Sumarah
Silage has become a key component of year-long animal feed in Canada and parts of northern Europe. It provides several advantages to farmers over traditional feed components, such as increased digestibility, higher nutrient content and preservation of the forages to meet seasonal feeding demands. Some ensiled materials can contain toxic fungal metabolites resulting from ‘in field’ contamination. In addition, when improperly stored or exposed to air during the feedout stage, silage is highly susceptible to aerobic spoilage by yeasts and filamentous fungi resulting in lower nutrient value and further mycotoxin contamination. In this study, silage samples were collected from 25 Canadian dairy goat and cattle farms where animals experienced feed-related health issues. Twenty-six unique fungal species were isolated from these samples, with the majority being Penicillium. High resolution liquid chromatography tandem mass spectrometry (HRLC-MS/MS) was used to identify a total of 125 known mycotoxins and fungal secondary metabolites from these silage samples, many of which were not produced by the 26 isolated filamentous fungi grown in agar cultures. Various mycotoxins resulting from preharvest contamination were detected, including ergot alkaloids, fumonisins and trichothecenes, some in high concentrations. Toxins produced after harvest included roquefortine C, citrinin and penitrem A. This study reinforces the need for farmers to implement best management practices to minimise fungal contamination and the resulting mycotoxin deposition in their crop and stored feed to maintain animal health.
{"title":"Mycotoxin determination in fungal contaminated Canadian silage toxic to dairy cows and goats","authors":"M. J. Kelman, J. Renaud, D. Baines, K. Yeung, J. Miller, M. Sumarah","doi":"10.3920/wmj2021.2764","DOIUrl":"https://doi.org/10.3920/wmj2021.2764","url":null,"abstract":"Silage has become a key component of year-long animal feed in Canada and parts of northern Europe. It provides several advantages to farmers over traditional feed components, such as increased digestibility, higher nutrient content and preservation of the forages to meet seasonal feeding demands. Some ensiled materials can contain toxic fungal metabolites resulting from ‘in field’ contamination. In addition, when improperly stored or exposed to air during the feedout stage, silage is highly susceptible to aerobic spoilage by yeasts and filamentous fungi resulting in lower nutrient value and further mycotoxin contamination. In this study, silage samples were collected from 25 Canadian dairy goat and cattle farms where animals experienced feed-related health issues. Twenty-six unique fungal species were isolated from these samples, with the majority being Penicillium. High resolution liquid chromatography tandem mass spectrometry (HRLC-MS/MS) was used to identify a total of 125 known mycotoxins and fungal secondary metabolites from these silage samples, many of which were not produced by the 26 isolated filamentous fungi grown in agar cultures. Various mycotoxins resulting from preharvest contamination were detected, including ergot alkaloids, fumonisins and trichothecenes, some in high concentrations. Toxins produced after harvest included roquefortine C, citrinin and penitrem A. This study reinforces the need for farmers to implement best management practices to minimise fungal contamination and the resulting mycotoxin deposition in their crop and stored feed to maintain animal health.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44150156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kagot, M. de Boevre, S. de Saeger, A. Moretti, M. Mwamuye, S. Okoth
Aspergillus and Fusarium are fungal genera that include toxigenic and pathogenic species, able to suffuse farmers’ crops and secrete an array of small molecular weight secondary metabolites which can cause health complications to humans and animals when ingested. In sub-Sahara Africa, contamination and persistence of these fungi is increased by the tropical climatic conditions which are ideal for the fungi to thrive. This study evaluated the incidence, regional distribution and toxigenic potential of Aspergillus and Fusarium species occurring in maize kernels from Eastern, Western, Coastal and the Lake Victoria agro-ecological zones of Kenya. Maize kernels were collected from 16 households in each agro-ecological zone. Single spore technique was used to isolate pure cultures of Aspergillus and Fusarium which were identified morphologically. Further, molecular analysis was done using the internal transcribed spacer 1 (ITS 1) region of the ribosomal DNA for Aspergillus and the translation elongation factor-1 alpha (TEF-1α) for Fusarium. The potential of the isolated fungi to produce mycotoxins was probed by polymerase chain reaction (PCR) based on the aflatoxin regulatory aflaR gene in Aspergillus, and the fumonisin backbone structure gene FUM1 in Fusarium. Among the potentially aflatoxigenic A. flavus species isolated, 55% were from Eastern, 27% from the Coastal zone, 13% from Lake Victoria zone and 5% from Western Kenya. Among the potentially fumonisin producing F. verticillioides isolated, 45% were from the Lake Victoria agro-ecological zone, 30% were from Western, 15% from Eastern Kenya and 10% from the Coastal agro-ecological zone. This study adds data on potential mycotoxin hotspots in Kenya useful in employing targeted and regional mycotoxin mitigation strategies in efforts to avert future mycotoxicoses outbreaks in Kenya.
{"title":"Incidence of toxigenic Aspergillus and Fusarium species occurring in maize kernels from Kenyan households","authors":"V. Kagot, M. de Boevre, S. de Saeger, A. Moretti, M. Mwamuye, S. Okoth","doi":"10.3920/wmj2021.2748","DOIUrl":"https://doi.org/10.3920/wmj2021.2748","url":null,"abstract":"Aspergillus and Fusarium are fungal genera that include toxigenic and pathogenic species, able to suffuse farmers’ crops and secrete an array of small molecular weight secondary metabolites which can cause health complications to humans and animals when ingested. In sub-Sahara Africa, contamination and persistence of these fungi is increased by the tropical climatic conditions which are ideal for the fungi to thrive. This study evaluated the incidence, regional distribution and toxigenic potential of Aspergillus and Fusarium species occurring in maize kernels from Eastern, Western, Coastal and the Lake Victoria agro-ecological zones of Kenya. Maize kernels were collected from 16 households in each agro-ecological zone. Single spore technique was used to isolate pure cultures of Aspergillus and Fusarium which were identified morphologically. Further, molecular analysis was done using the internal transcribed spacer 1 (ITS 1) region of the ribosomal DNA for Aspergillus and the translation elongation factor-1 alpha (TEF-1α) for Fusarium. The potential of the isolated fungi to produce mycotoxins was probed by polymerase chain reaction (PCR) based on the aflatoxin regulatory aflaR gene in Aspergillus, and the fumonisin backbone structure gene FUM1 in Fusarium. Among the potentially aflatoxigenic A. flavus species isolated, 55% were from Eastern, 27% from the Coastal zone, 13% from Lake Victoria zone and 5% from Western Kenya. Among the potentially fumonisin producing F. verticillioides isolated, 45% were from the Lake Victoria agro-ecological zone, 30% were from Western, 15% from Eastern Kenya and 10% from the Coastal agro-ecological zone. This study adds data on potential mycotoxin hotspots in Kenya useful in employing targeted and regional mycotoxin mitigation strategies in efforts to avert future mycotoxicoses outbreaks in Kenya.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45166872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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