Ochratoxin A (OTA) is a possible human carcinogen commonly found in various agricultural commodities worldwide. While this potent nephrotoxin tends to survive common food processes and contaminate food products, certain process with higher temperature treatments may reduce OTA contents. Roasting has been suggested as a possible method to reduce OTA in coffee beans with up to 90% reduction, which may be applied to other food commodities. In this study, the possible influence of fibres on the reduction of OTA was investigated with brown and white rice with 2.2 and 6.7% of total dietary fibre content, respectively, roasting at two different temperatures (160 and 200 °C) for up to 30 min. The results showed that the rate and extent of OTA reduction were dependent on time, temperature, and rice type; greater than 60% of OTA reduction were achieved at 200 °C for 30 min for white rice and 37% for brown rice at the same conditions. No significant differences in reduction were observed between the samples roasted at 160 °C for 30 min and 200 °C for 15 min for both the white and brown rice, while white rice roasted at 160 °C for 15 min during roasting may be affected by the presence of fibre and/or fat in the cereal grains.
{"title":"Varied reduction of ochratoxin A in brown and white rice during roasting","authors":"H.N. Carbon, H. Lee","doi":"10.3920/wmj2021.2712","DOIUrl":"https://doi.org/10.3920/wmj2021.2712","url":null,"abstract":"Ochratoxin A (OTA) is a possible human carcinogen commonly found in various agricultural commodities worldwide. While this potent nephrotoxin tends to survive common food processes and contaminate food products, certain process with higher temperature treatments may reduce OTA contents. Roasting has been suggested as a possible method to reduce OTA in coffee beans with up to 90% reduction, which may be applied to other food commodities. In this study, the possible influence of fibres on the reduction of OTA was investigated with brown and white rice with 2.2 and 6.7% of total dietary fibre content, respectively, roasting at two different temperatures (160 and 200 °C) for up to 30 min. The results showed that the rate and extent of OTA reduction were dependent on time, temperature, and rice type; greater than 60% of OTA reduction were achieved at 200 °C for 30 min for white rice and 37% for brown rice at the same conditions. No significant differences in reduction were observed between the samples roasted at 160 °C for 30 min and 200 °C for 15 min for both the white and brown rice, while white rice roasted at 160 °C for 15 min during roasting may be affected by the presence of fibre and/or fat in the cereal grains.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43366882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Narváez, Y. Rodríguez-Carrasco, A. Ritieni, J. Mañes
Human biomonitoring (HBM) represents the most accurate approach for assessing the exposure to mycotoxins, but traditional matrices fail to provide information about long-term exposure due to the rapid excretion rates and short half-lives of mycotoxins. Hair emerges as a promising matrix considering that contaminants can form stable links with hair components, such as keratins and melanin. Hence, the aim of the present study was to monitor the presence of up to ten mycotoxins (aflatoxins and Fusarium mycotoxins) in human hair samples (n=100) through a high-performance liquid chromatography coupled to Q-TOF high resolution mass spectrometry. A prevalence of 43% at concentrations ranging from 2.7 to 106.1 ng/g was observed, being enniatins and aflatoxin B1 the most prevalent compounds. Co-occurrence of up to three mycotoxins was observed in 42% of the positive samples. Retrospective untargeted analysis of hair samples tentatively identified up to 128 mycotoxins and related metabolites. These results confirm the accumulation of toxicologically relevant mycotoxins in hair matrix, thus standing as a suitable matrix for assessing long-term exposure.
{"title":"Human biomonitoring of multiple mycotoxins in hair: first large-scale pilot study","authors":"A. Narváez, Y. Rodríguez-Carrasco, A. Ritieni, J. Mañes","doi":"10.3920/wmj2021.2744","DOIUrl":"https://doi.org/10.3920/wmj2021.2744","url":null,"abstract":"Human biomonitoring (HBM) represents the most accurate approach for assessing the exposure to mycotoxins, but traditional matrices fail to provide information about long-term exposure due to the rapid excretion rates and short half-lives of mycotoxins. Hair emerges as a promising matrix considering that contaminants can form stable links with hair components, such as keratins and melanin. Hence, the aim of the present study was to monitor the presence of up to ten mycotoxins (aflatoxins and Fusarium mycotoxins) in human hair samples (n=100) through a high-performance liquid chromatography coupled to Q-TOF high resolution mass spectrometry. A prevalence of 43% at concentrations ranging from 2.7 to 106.1 ng/g was observed, being enniatins and aflatoxin B1 the most prevalent compounds. Co-occurrence of up to three mycotoxins was observed in 42% of the positive samples. Retrospective untargeted analysis of hair samples tentatively identified up to 128 mycotoxins and related metabolites. These results confirm the accumulation of toxicologically relevant mycotoxins in hair matrix, thus standing as a suitable matrix for assessing long-term exposure.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47786206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S.A. Tittlemier, B. Cramer, C. Dall’Asta, M.C. DeRosa, V.M.T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M.W. Sumarah
This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.
{"title":"Developments in mycotoxin analysis: an update for 2020-2021","authors":"S.A. Tittlemier, B. Cramer, C. Dall’Asta, M.C. DeRosa, V.M.T. Lattanzio, R. Malone, C. Maragos, M. Stranska, M.W. Sumarah","doi":"10.3920/wmj2021.2752","DOIUrl":"https://doi.org/10.3920/wmj2021.2752","url":null,"abstract":"This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138495093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Ghafari, G. Paimard, E. Sadeghi, N. Choobkar, M. A. Lalabadi
In the present study, we report three methods of silica nanoparticles (SNPs) as adsorbent, ultraviolet (UV) irradiation, and microwave heating and evaluate their capabilities in reducing and eliminating zearalenone (ZEN). The offered method not only was used for ZEN detoxification, but also greatly enhanced the sensitivity of ZEN measurement. The aim of this study was to evaluate ZEN concentration in sunflower oil samples by high-performance liquid chromatography (HPLC) method. This method was successfully validated for sunflower oil samples while the limit of detection (LOD) method (signal-to-noise ratio of 3:1) was 0.5 μg/l. The acquired removal data with the HPLC method through SNPs were fitted well with Freundlich isotherm, denoting that the multi-layer adsorption took place on the adsorbent. The equilibrium adsorption capacity of ZEN was 61.02 μg/g in an optimum time of 240 min on SNPs. The experimental results were evaluated by the adsorption kinetic model, which specified the adsorption kinetics of ZEN on SNPs, obeying the pseudo-second order model. This model demonstrated that the sorption rate depended on the sorption capacity but not the concentration of the sorbate. Moreover, the method presented to determine ZEN based on the use of SNPs in sunflower oil was accomplished by the adsorption process. Furthermore, the removal efficiencies of ZEN by SNPs, UV irradiation, and microwave heating were compared and obtained to be 92.1, 96.22, and 37.30%, respectively for determined times. These results confirm the removal efficiency of these methods is sensitive enough to ZEN analysis in sunflower oil samples.
{"title":"Evaluation of nano-silica, microwave heating, and ultraviolet irradiation effects on zearalenone detoxification in sunflower oils","authors":"N. Ghafari, G. Paimard, E. Sadeghi, N. Choobkar, M. A. Lalabadi","doi":"10.3920/wmj2021.2733","DOIUrl":"https://doi.org/10.3920/wmj2021.2733","url":null,"abstract":"In the present study, we report three methods of silica nanoparticles (SNPs) as adsorbent, ultraviolet (UV) irradiation, and microwave heating and evaluate their capabilities in reducing and eliminating zearalenone (ZEN). The offered method not only was used for ZEN detoxification, but also greatly enhanced the sensitivity of ZEN measurement. The aim of this study was to evaluate ZEN concentration in sunflower oil samples by high-performance liquid chromatography (HPLC) method. This method was successfully validated for sunflower oil samples while the limit of detection (LOD) method (signal-to-noise ratio of 3:1) was 0.5 μg/l. The acquired removal data with the HPLC method through SNPs were fitted well with Freundlich isotherm, denoting that the multi-layer adsorption took place on the adsorbent. The equilibrium adsorption capacity of ZEN was 61.02 μg/g in an optimum time of 240 min on SNPs. The experimental results were evaluated by the adsorption kinetic model, which specified the adsorption kinetics of ZEN on SNPs, obeying the pseudo-second order model. This model demonstrated that the sorption rate depended on the sorption capacity but not the concentration of the sorbate. Moreover, the method presented to determine ZEN based on the use of SNPs in sunflower oil was accomplished by the adsorption process. Furthermore, the removal efficiencies of ZEN by SNPs, UV irradiation, and microwave heating were compared and obtained to be 92.1, 96.22, and 37.30%, respectively for determined times. These results confirm the removal efficiency of these methods is sensitive enough to ZEN analysis in sunflower oil samples.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41301190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the Great Lakes region of North America, Gibberella ear rot (GER), caused by Fusarium graminearum, affects grain quality due to the accumulation of mycotoxins. GER severity is strongly influenced by environmental conditions; however, agronomic practices can also influence disease severity and mycotoxin accumulation. In this study, three separate small-plot experiments were conducted at Ridgetown, ON, Canada during 2019 and 2020 under an inoculated-misted system to determine Fusarium mycotoxin accumulation as affected by: (1) plant population density; (2) in-row-plant developmental variability; and (3) the effect of integrated Bt refuge genetics. In this study, DON concentrations were at least 49% higher in maize at 113,600 plants/ha compared to 79,000 plants/ha. Moreover, mycotoxin accumulation was higher in plants that were delayed developmentally in the crop row; total DON concentrations were at least 310% higher in late silked plants adjacent to early silked plants. Results of the plant population density and in-row-plant developmental variability suggest that the main driver for mycotoxin accumulation was stress induced by plant competition rather than environmental conditions; this highlights the importance of avoiding plant competitive stress as a strategy to reduce the risks of mycotoxin accumulation. In this study, there was no statistical difference in DON accumulation between the Bt component and the non-Bt component in each of the four hybrids tested; however, there was evidence that hybrids varied in susceptibility, including the Bt and non-Bt components that were paired commercially in a bag of seed maize. Reducing mycotoxins in maize requires integrated management, which includes agronomic considerations. These results indicate that mycotoxins are favoured with high plant populations and plant-to-plant variability in the row, especially in susceptible hybrids.
{"title":"Impact of agronomic practices on Fusarium mycotoxin accumulation in maize grain","authors":"K. Eli, A. Schaafsma, D. Hooker","doi":"10.3920/wmj2021.2734","DOIUrl":"https://doi.org/10.3920/wmj2021.2734","url":null,"abstract":"In the Great Lakes region of North America, Gibberella ear rot (GER), caused by Fusarium graminearum, affects grain quality due to the accumulation of mycotoxins. GER severity is strongly influenced by environmental conditions; however, agronomic practices can also influence disease severity and mycotoxin accumulation. In this study, three separate small-plot experiments were conducted at Ridgetown, ON, Canada during 2019 and 2020 under an inoculated-misted system to determine Fusarium mycotoxin accumulation as affected by: (1) plant population density; (2) in-row-plant developmental variability; and (3) the effect of integrated Bt refuge genetics. In this study, DON concentrations were at least 49% higher in maize at 113,600 plants/ha compared to 79,000 plants/ha. Moreover, mycotoxin accumulation was higher in plants that were delayed developmentally in the crop row; total DON concentrations were at least 310% higher in late silked plants adjacent to early silked plants. Results of the plant population density and in-row-plant developmental variability suggest that the main driver for mycotoxin accumulation was stress induced by plant competition rather than environmental conditions; this highlights the importance of avoiding plant competitive stress as a strategy to reduce the risks of mycotoxin accumulation. In this study, there was no statistical difference in DON accumulation between the Bt component and the non-Bt component in each of the four hybrids tested; however, there was evidence that hybrids varied in susceptibility, including the Bt and non-Bt components that were paired commercially in a bag of seed maize. Reducing mycotoxins in maize requires integrated management, which includes agronomic considerations. These results indicate that mycotoxins are favoured with high plant populations and plant-to-plant variability in the row, especially in susceptible hybrids.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42546748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F.G. Silva, L. Zanin, C. F. Shimizu, D. D. Lopes, J. C. Ribeiro, A. T. Ishikawa, E. N. Itano, O. Kawamura, E. Y. Hirooka
An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.
{"title":"Aflatoxin B1 in the egg chain: monitoring with specific indirect competitive ELISA in northern Paraná, Brazil","authors":"F.G. Silva, L. Zanin, C. F. Shimizu, D. D. Lopes, J. C. Ribeiro, A. T. Ishikawa, E. N. Itano, O. Kawamura, E. Y. Hirooka","doi":"10.3920/wmj2021.2728","DOIUrl":"https://doi.org/10.3920/wmj2021.2728","url":null,"abstract":"An indirect competitive immunoassay (ic-ELISA) was developed using monoclonal antibody produced by hybridoma AF4, which showed high specificity and reactivity with aflatoxin B1 (AFB1) and aflatoxicol, but low cross-reactivity to other analogs. This low cost reliable method was applied for AFB1 monitoring in the poultry chain of a high agribusiness potential region (northern Paraná state, Brazil). Maize, laying hens feed and egg samples were collected from two poultry farms (with production above 200,000 eggs/day) and evaluated by intralaboratory validated ic-ELISA. The sensitivity of such a validated assay, detecting picogram levels of aflatoxins, demonstrated to be proper for surveying daily ingested cumulative toxins and estimating risks. Additionally, more than 61.00% of positive egg samples ranged between the limit of quantification (LOQ – 0.035 ng/g) and 1.00 ng/g, values commonly not covered by commercial kits. Positive data (>LOQ) occurred in 22 maize (56.40%), 34 feed (85.00%) and 192 (48.00%) egg samples. Mean contamination in maize was 1.51±0.94 ng/g (range 0.11-3.91 ng/g), 1.26±0.96 ng/g in feed (0.10-3.58 ng/g), and 1.01±0.77 ng/g in egg (0.05-3.85 ng/g). No statistical difference was observed between farms (P>0.05) for any of the matrices analysed. However, the difference between median values in maize (0.98 ng/g – Farm A; 1.76 ng/g – Farm B) indicated a higher contamination trend in farm B, possibly due to inadequate local storage. Although there is no limit stipulated for AFB1 contamination in eggs, the levels detected in samples were low and do not represent an immediate risk to animal production or human consumption. Nevertheless, the high frequency of positive maize and feed samples in this field of agribusiness should be highlighted. Sensitive aflatoxin monitoring procedures must be strategically carried out from raw materials to animal derived products, aiming harmless production, which also assures human health.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48191873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Hamad, H. A. El-Makarem, A. Elaziz, A. Amer, B. El-Nogoumy, S. Abou-Alella
The incidence of the mycotoxin ochratoxin A (OTA) in cheeses constitutes a significant economic and health concern for producers and consumers alike. Recently, detoxification approaches using food additives to counteract mycotoxins have been widely recommended in the food industry. This study aimed to quantify OTA levels in some Egyptian cheese types, and experimentally determine the detoxification effect of bentonite both in vitro and in vivo. The examined Roomy and Karish cheese showed higher OTA levels (4.138 and 3.399 μg/kg, respectively) than other cheeses. Calcium bentonite presented higher adsorption efficiency than sodium bentonite at all concentrations, both in phosphate buffered saline (PBS) and feta cheese, and at the whole pH range. Calcium bentonite concentrations (60 and 100 mg/ml) had much higher sequestering activity on OTA both in PBS and feta cheese, while the adsorption efficiency was higher at pH 6.8 than at pH 3. All enzymatic activities were near the control levels in rats treated both with OTA and bentonite compared with rats treated with OTA alone. The IC50 of calcium bentonite was 107.75 μg/ml, which was less cytotoxic than sodium bentonite (52.96 μg/ml). Bentonites were categorised by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) as excellent binders of OTA. The innovative calcium bentonite-fortified feta cheese showed the most superior sensorial properties; hence it can be predicted as a novel food-grade adsorbent for OTA sequestration.
{"title":"Adsorption efficiency of sodium & calcium bentonite for ochratoxin A in some Egyptian cheeses: an innovative fortification model, in vitro and in vivo experiments","authors":"G. Hamad, H. A. El-Makarem, A. Elaziz, A. Amer, B. El-Nogoumy, S. Abou-Alella","doi":"10.3920/wmj2021.2682","DOIUrl":"https://doi.org/10.3920/wmj2021.2682","url":null,"abstract":"The incidence of the mycotoxin ochratoxin A (OTA) in cheeses constitutes a significant economic and health concern for producers and consumers alike. Recently, detoxification approaches using food additives to counteract mycotoxins have been widely recommended in the food industry. This study aimed to quantify OTA levels in some Egyptian cheese types, and experimentally determine the detoxification effect of bentonite both in vitro and in vivo. The examined Roomy and Karish cheese showed higher OTA levels (4.138 and 3.399 μg/kg, respectively) than other cheeses. Calcium bentonite presented higher adsorption efficiency than sodium bentonite at all concentrations, both in phosphate buffered saline (PBS) and feta cheese, and at the whole pH range. Calcium bentonite concentrations (60 and 100 mg/ml) had much higher sequestering activity on OTA both in PBS and feta cheese, while the adsorption efficiency was higher at pH 6.8 than at pH 3. All enzymatic activities were near the control levels in rats treated both with OTA and bentonite compared with rats treated with OTA alone. The IC50 of calcium bentonite was 107.75 μg/ml, which was less cytotoxic than sodium bentonite (52.96 μg/ml). Bentonites were categorised by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) as excellent binders of OTA. The innovative calcium bentonite-fortified feta cheese showed the most superior sensorial properties; hence it can be predicted as a novel food-grade adsorbent for OTA sequestration.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42284573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Phillips, P. Turner, F. Ngure, N. Kassim, E. Makule, L. E. Smith, R. Nelson, R. Stoltzfus
Aflatoxins are fungal metabolites that commonly contaminate staple food crops in tropical regions. Acute aflatoxin consumption in very high concentration causes aflatoxicosis and acute liver failure, while chronic, moderate levels of intake cause hepatocellular carcinoma. The effects of frequent moderate- to high-level exposure during infancy, however, is less clearly understood. Half a billion people in low- and middle-income countries continue to be exposed to aflatoxins through dietary consumption, in part because of lack of enforcement of regulatory limits and few feasible long-term mitigation options in these settings. Several epidemiologic studies have shown an association between aflatoxin exposure in infants and young children and growth failure, but strong experimental evidence is lacking. The Mycotoxin Mitigation Trial conducted in Tanzania was a cluster-randomised trial to assess the effect of a reduced aflatoxin diet on linear growth. Prior to the design and implementation of this trial, a group of multi-disciplinary and multi-national scientists reviewed literature in biomedical, public health, environmental health ethics. In this paper we outline the most salient ethical questions and dilemmas in the potential conduct of such a study and describe the ethical precedents and principles that informed our decision-making processes and ultimate study protocol.
{"title":"Ethical considerations in the design and conduct of a cluster-randomised mycotoxin mitigation trial in Tanzania","authors":"E. Phillips, P. Turner, F. Ngure, N. Kassim, E. Makule, L. E. Smith, R. Nelson, R. Stoltzfus","doi":"10.3920/wmj2021.2705","DOIUrl":"https://doi.org/10.3920/wmj2021.2705","url":null,"abstract":"Aflatoxins are fungal metabolites that commonly contaminate staple food crops in tropical regions. Acute aflatoxin consumption in very high concentration causes aflatoxicosis and acute liver failure, while chronic, moderate levels of intake cause hepatocellular carcinoma. The effects of frequent moderate- to high-level exposure during infancy, however, is less clearly understood. Half a billion people in low- and middle-income countries continue to be exposed to aflatoxins through dietary consumption, in part because of lack of enforcement of regulatory limits and few feasible long-term mitigation options in these settings. Several epidemiologic studies have shown an association between aflatoxin exposure in infants and young children and growth failure, but strong experimental evidence is lacking. The Mycotoxin Mitigation Trial conducted in Tanzania was a cluster-randomised trial to assess the effect of a reduced aflatoxin diet on linear growth. Prior to the design and implementation of this trial, a group of multi-disciplinary and multi-national scientists reviewed literature in biomedical, public health, environmental health ethics. In this paper we outline the most salient ethical questions and dilemmas in the potential conduct of such a study and describe the ethical precedents and principles that informed our decision-making processes and ultimate study protocol.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47068619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fumonisin B1 (FB1) contributes to mycotoxicosis in animals and has been associated with the incidence of some cancers in humans. The effect of FB1 on lipidomic profiles, sphingolipids and cholesterol levels have been demonstrated in experimental models, however, the events leading to altered cholesterol levels are unclear. This study investigates the molecular mechanisms that regulate the effect of FB1 on cholesterol homeostasis in galactose supplemented HepG2 liver cells. Galactose supplementation is a proven method utilised to circumvent the Crabtree effect exhibited by cancer cells, which forces cancer cells to activate the mitochondria. HepG2 cells were cultured in galactose supplemented media and treated with FB1 (IC50 = 25 μM) for 6 h. Cell viability was determined using the MTT assay. Metabolic status was evaluated using ATP luciferase assay, and cholesterol regulatory transcription factors (SIRT1, SREBP-1C, LXR, LDLR, PCSK9, and ABCA1) were investigated using western blotting and qPCR. FB1 in galactose supplemented HepG2 cells increased gene expression of SIRT1 (P<0.05), SREBP-1C, LXR, and LDLR; however, PCSK9 (P<0.05) was decreased. Furthermore, protein expression of SIRT1, LXR, and LDLR was elevated upon FB1 treatment, while SREBP-1C and PCSK9 were reduced. The data provides evidence that SIRT1 reduced the expression of PCSK9 and deacetylated LXR to prevent degradation of LDLR. This could result in a dysregulated cholesterol flux, which may contribute to FB1 mediated toxicity.
{"title":"HepG2 liver cells treated with fumonisin B1 in galactose supplemented media have altered expression of genes and proteins known to regulate cholesterol flux","authors":"T. Adimulam, N. S. Abdul, A. Chuturgoon","doi":"10.3920/wmj2021.2723","DOIUrl":"https://doi.org/10.3920/wmj2021.2723","url":null,"abstract":"Fumonisin B1 (FB1) contributes to mycotoxicosis in animals and has been associated with the incidence of some cancers in humans. The effect of FB1 on lipidomic profiles, sphingolipids and cholesterol levels have been demonstrated in experimental models, however, the events leading to altered cholesterol levels are unclear. This study investigates the molecular mechanisms that regulate the effect of FB1 on cholesterol homeostasis in galactose supplemented HepG2 liver cells. Galactose supplementation is a proven method utilised to circumvent the Crabtree effect exhibited by cancer cells, which forces cancer cells to activate the mitochondria. HepG2 cells were cultured in galactose supplemented media and treated with FB1 (IC50 = 25 μM) for 6 h. Cell viability was determined using the MTT assay. Metabolic status was evaluated using ATP luciferase assay, and cholesterol regulatory transcription factors (SIRT1, SREBP-1C, LXR, LDLR, PCSK9, and ABCA1) were investigated using western blotting and qPCR. FB1 in galactose supplemented HepG2 cells increased gene expression of SIRT1 (P<0.05), SREBP-1C, LXR, and LDLR; however, PCSK9 (P<0.05) was decreased. Furthermore, protein expression of SIRT1, LXR, and LDLR was elevated upon FB1 treatment, while SREBP-1C and PCSK9 were reduced. The data provides evidence that SIRT1 reduced the expression of PCSK9 and deacetylated LXR to prevent degradation of LDLR. This could result in a dysregulated cholesterol flux, which may contribute to FB1 mediated toxicity.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44597184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mycotoxins are secondary metabolites produced by fungi including Aspergillus and Fusarium that commonly contaminate crops, such as maize, resulting in economic losses and food insecurity. Mycotoxins can contaminate crops during pre- and post-harvest stages. Consumption of mycotoxin-contaminated foods has been linked to a variety of negative health outcomes including liver cancer, stunting, and neural tube defects. In countries such as Guatemala where maize constitutes a major portion of the diet, mycotoxins can be a significant contributor to disease burden. This review describes maize pre- and post-harvest practices in Guatemala that can lead to the development of mycotoxins and subsequent exposure to humans and animals, current information gaps, and opportunities for future research. There are specific challenges to minimising fungal growth and subsequent mycotoxin production during storage of maize in Guatemala, including reducing moisture content, minimising pest damage, and controlling temperature. Research on maize-handling practices that are associated with the greatest mycotoxin exposure in Guatemala is needed to prioritise allocation of resources and reduce exposure.
{"title":"Evaluation of the impact of pre- and post-harvest maize handling practices on mycotoxin contamination on smallholder farms in Guatemala","authors":"A. Garsow, D. Mendez, O. Torres, B. Kowalcyk","doi":"10.3920/wmj2021.2701","DOIUrl":"https://doi.org/10.3920/wmj2021.2701","url":null,"abstract":"Mycotoxins are secondary metabolites produced by fungi including Aspergillus and Fusarium that commonly contaminate crops, such as maize, resulting in economic losses and food insecurity. Mycotoxins can contaminate crops during pre- and post-harvest stages. Consumption of mycotoxin-contaminated foods has been linked to a variety of negative health outcomes including liver cancer, stunting, and neural tube defects. In countries such as Guatemala where maize constitutes a major portion of the diet, mycotoxins can be a significant contributor to disease burden. This review describes maize pre- and post-harvest practices in Guatemala that can lead to the development of mycotoxins and subsequent exposure to humans and animals, current information gaps, and opportunities for future research. There are specific challenges to minimising fungal growth and subsequent mycotoxin production during storage of maize in Guatemala, including reducing moisture content, minimising pest damage, and controlling temperature. Research on maize-handling practices that are associated with the greatest mycotoxin exposure in Guatemala is needed to prioritise allocation of resources and reduce exposure.","PeriodicalId":23844,"journal":{"name":"World Mycotoxin Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47758134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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