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[Analysis of cytogenetic indexes and occupational characteristics of radiation workers with lens opacity]. [患有晶状体混浊的放射工作人员的细胞遗传学指标和职业特点分析]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20230609-00200
Y F Bian, H C Zhong, Q Jin, P P Pan

Objective: To analyse the characteristics of micronuclus and chromosomal aberrations and the lens opacity in different working years, ages and work type of radiation workers with lens opacity. Methods: In January 2022, we selected 400 radiation workers with lens opacity who had undergone occupational health examination in Hangzhou Hospital for the Prevention and Treatment of Occupational Disease from March 2014 to December 2021 as research objects. To analyze the differences of micronucleus, chromosome aberration and the lens opacification rates of different length of service, type of work, age radiologic workers. Results: The results showed that radiation the micronucleus abnormality (0.59‰) and chromosome aberration (2.67%) in peripheral blood in workers who served more than 20 years were significantly higher than that of micronucleus abnormality (0.18‰) and chromosome aberration (0.31%) in workers served less than 20 years, the differences were statistically significant (P<0.05). The micronucleus abnormality (0.32‰) and chromosome aberration (0.57%) in the interventionist were higher than oral radiation workers (0.12‰ and 0.19%) and diagnostic radiologist (0.05‰ and 0.12%), the differences were statistically signcficant (χ(2)=23.98, 6.72, P<0.05) . Conclusion: The rates of micronucleus and chromosome aberration of the interventionaist are higher than oral radiation workers and the traditional radiologist. Workers engaged in radiation should improve personal protection and undertake regular occupational health examinations.

目的分析患有晶状体混浊的放射工作人员在不同工作年限、年龄和工种下的微小畸形、染色体畸变和晶状体混浊的特征。方法: 2022 年 1 月,我们选取了 400 名患有晶状体混浊的放射工作人员:2022年1月,选取2014年3月至2021年12月在杭州市职业病防治院进行职业健康检查的400名晶状体混浊放射工作人员作为研究对象。分析不同工龄、工种、年龄放射工作人员微核、染色体畸变及晶状体混浊率的差异。结果显示结果显示,工龄在 20 年以上的放射工作人员外周血微核异常率(0.59‰)和染色体畸变率(2.67%)明显高于工龄在 20 年以下的放射工作人员外周血微核异常率(0.18‰)和染色体畸变率(0.31%),差异有统计学意义(PPConclusion:介入放射工作者的微核和染色体畸变率高于口腔放射工作者和传统放射工作者。从事放射工作的人员应加强个人防护,定期进行职业健康检查。
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引用次数: 0
[Analysis of disease burden and trends of noise-induced hearing loss in China from 1990 to 2019]. [1990-2019年中国噪声性听力损失的疾病负担和趋势分析]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20240109-00119
L Chen, Y Liao, Z B Cen, J W Zhang, M H Xiao, Z Wang

Objective: To analyze the disease burden of Noise-Induced Hearing Loss (NIHL) in China from 1990 to 2019, forecast the disease burden of NIHL from 2020 to 2030, and provide data support for the prevention and control of NIHL. Methods: In July 2022, the disease burden data of NIHL in different age groups and genders in China during 1990-2019 were selected from the GBD database. The Jointpoint regression model was established to analyze the trend of the disease burden of NIHL in China. An age-period-cohort model was constructed to analyze the changing trend of NIHL in terms of age, period, and cohort, and a Bayesian age-period-cohort model was developed to predict the disease burden of NIHL in China from 2020 to 2030. Results: From 1990 to 2019, the disability adjusted life year (DALY) of China's NIHL increased from 1361600 to 2327700 years. The coarse rate of DALY increased from 115.03/100000 to 163.65/100000 (AAPC=1.23, P<0.001), and the normalization rate of DALY decreased from 127.67/100000 to 119.83/100000 (AAPC=-0.21, P<0.001). It is predicted that from 2020 to 2030, the DALYs of China's NIHL will increase from 2412900 in 2020 to 2655000 in 2030, and the DALY normalization rate will decrease from 241.29/100000 in 2020 to 125.71/100000 in 2030. Conclusion: The burden of noise-induced hearing loss (NIHL) in China is significant and should not be overlooked. To reduce this burden, we need to focus on strengthening source management, process control, personal protection, and comprehensive prevention and treatment through various methods.

目的分析1990-2019年中国噪声性听力损失(NIHL)的疾病负担,预测2020-2030年NIHL的疾病负担,为NIHL的防控提供数据支持。研究方法2022年7月,从GBD数据库中选取1990-2019年中国不同年龄组和性别的NIHL疾病负担数据。建立联合点回归模型,分析中国 NIHL 疾病负担的变化趋势。建立年龄-时期-队列模型,分析NIHL在年龄、时期和队列方面的变化趋势,并建立贝叶斯年龄-时期-队列模型,预测2020-2030年中国NIHL的疾病负担。结果显示从 1990 年到 2019 年,中国 NIHL 的残疾调整生命年(DALY)从 1361600 年增加到 2327700 年。DALY的粗比率从115.03/100000增加到163.65/100000(AAPC=1.23,PPC结论):在中国,噪声性听力损失(NIHL)造成的负担十分沉重,不容忽视。为了减轻这一负担,我们需要重点加强源头管理、过程控制、个人防护,并通过各种方法进行综合防治。
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引用次数: 0
[Current status of research on the diagnosis and treatment of acute ethylene glycol poisoning]. [急性乙二醇中毒的诊断和治疗研究现状]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20231106-00101
K Yang, Hogahemat Xiao Kelaiti, P Peng

Acute ethylene glycol poisoning often leads to acute kidney injury, and delays in diagnosis and treatment can lead to severe multi-organ dysfunction and even death. At present, there is a lack of specific laboratory tests that can be used for rapid clinical diagnosis, and diagnosis and evaluation only rely on medical history, clinical symptoms, and non-specific laboratory test results. Key treatments include alcohol dehydrogenase inhibitors (fomcpizole or ethanol) and hemodialysis, which is not approved in China. A clear understanding of the mechanism of toxicity, pathophysiology, clinical manifestations, efficacy and limitations of laboratory tests, and treatment options after ethylene glycol poisoning can help accelerate the early diagnosis, treatment, and prognosis of patients after ethylene glycol poisoning. This article reviews this article in order to provide a reference for clinicians.

急性乙二醇中毒通常会导致急性肾损伤,延误诊断和治疗会导致严重的多器官功能障碍,甚至死亡。目前,缺乏可用于临床快速诊断的特异性实验室检查,诊断和评估只能依靠病史、临床症状和非特异性实验室检查结果。主要治疗方法包括酒精脱氢酶抑制剂(福美唑或乙醇)和血液透析,但血液透析在中国尚未获得批准。清楚地了解乙二醇中毒后的中毒机制、病理生理学、临床表现、实验室检查的有效性和局限性以及治疗方案,有助于加快乙二醇中毒后患者的早期诊断、治疗和预后。本文对此进行了综述,以期为临床医生提供参考。
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引用次数: 0
[Diagnosis and management of work related musculoskeletal disorders as occupational diseases in Germany]. [德国与工作有关的肌肉骨骼疾病作为职业病的诊断和管理]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20230926-00070
Z L Chen, J C Li, L Wu, X Y Dai, S Q Chen, L Yang

This article introduces the occupational disease diagnosis of work-related musculoskeletal disorders such as lumbar and spinal injuries in Germany, and focuses on the research and standard values of the maximum pressure tolerance of intervertebral discs. This article mainly introduces the dose model limits and their applications proposed by the Mainz Dortmund Dosis model (MDD), the judgment of the German Social Court (Bundessozialgericht, BSG), and the German Spinal Research Re evaluation (Deutsche Wirbels ulentudieⅡ, DWSⅡ) ; X-ray manifestations and measurements of intervertebral disc injury; Problems in actual cases and diagnosis. In order to provide reference and inspiration for the occupational diseases diagnosis of work related musculoskeletal disorders in China.

本文介绍了德国与工作相关的腰椎和脊柱损伤等肌肉骨骼疾病的职业病诊断,并重点介绍了椎间盘最大耐压的研究和标准值。本文主要介绍了美因茨多特蒙德剂量模型(MDD)提出的剂量模型限值及其应用、德国社会法院(BSG)的判决和德国脊柱研究再评估(DWSⅡ);椎间盘损伤的X射线表现和测量;实际病例中存在的问题及诊断。为我国与工作相关的肌肉骨骼疾病的职业病诊断提供参考和启示。
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引用次数: 0
[A case report of pulmonary aspergillosis secondary to occupational chronic formaldehyde toxic obstructive pulmonary disease]. [职业性慢性甲醛中毒性阻塞性肺病继发肺曲霉病病例报告]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20230713-00247
Z P Dong, Z M Fan, H Y Shao, Y X Zhao

Invasive pulmonary aspergillosis is the most common type of pulmonary aspergillosis. This paper reported a patient with pulmonary aspergillosis secondary to obstructive pulmonary disease and other underlying diseases. The clinical manifestations included wheezing, cough, fever and wheezing rale in the lungs. Diagnosis was ultimately confirmed through pathogens targeted next generation sequencing and pathological examination of respiratory coughs. Following comprehensive treatment that included antifungal therapy, the patient was cured and discharged with a good prognosis.

侵袭性肺曲霉菌病是最常见的肺曲霉菌病类型。本文报告了一名继发于阻塞性肺病和其他基础疾病的肺曲霉菌病患者。临床表现包括喘息、咳嗽、发热和肺部喘息性啰音。通过病原体靶向新一代测序和呼吸道咳嗽的病理检查,最终确诊了该病。经过包括抗真菌治疗在内的综合治疗后,患者痊愈出院,预后良好。
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引用次数: 0
[A meta-analysis of the prevalence of work-related musculoskeletal disorders among rehabilitation therapists]. [康复治疗师中与工作相关的肌肉骨骼疾病发病率的荟萃分析]。
Q3 Medicine Pub Date : 2024-10-20 DOI: 10.3760/cma.j.cn121094-20231024-00093
J Pan, M C Wang, S Y Zhao, Q Liu, H Y Zhai

Objective: To investigate the prevalence of work-related musculoskeletal disorders (WMSDs) in rehabilitation therapists. Methods: In September 2023, the computer searched Chinese and English databases such as CNKI database, Wanfang Data Knowledge Service Platform, VIP Chinese Science and Technology Journal Database, Chinese Biomedical Literature Database, PubMed, Cochrane Library, Web of Science, Embase database, etc. The retrieval time was from the establishment of the database to September 2023. Musculoskeletal disorders, rehabilitation therapists were selected as search terms, and the author, publication time, prevalence rate, and study type were analyzed. The Agency for Healthcare Research and Quality (AHRQ) tool and The Newcastle-Ottawa Scale (NOS) were used to evaluate the quality of the literature. Stata 15.0 software was used for meta-analysis. Results: A total of 28 literatures were included, including 8039 rehabilitation therapists. Meta-analysis showed that the overall prevalence rate of WMSDs among rehabilitation therapists was 72% (95%CI: 64%-80%). The top 3 annual prevalence rates were lower back (51%, 95%CI: 38%-64%), neck (42%, 95%CI: 30%-54%) and shoulder (32%, 95%CI: 23%-41%). The top 3 weekly prevalence rates were lower back (47%, 95%CI: 28%-65%), neck (35%, 95%CI: 14%-55%) and back (30%, 95% CI: 17%-44%) . Conclusion: The higher prevalence of WMSDs in rehabilitation therapists, especially in the lower back, neck, shoulder and back, may be related to the prolonged stouching posture and the continuous or repeated exertion of the upper limbs.

目的调查康复治疗师中与工作相关的肌肉骨骼疾病(WMSDs)的发病率。方法2023年9月,计算机检索CNKI数据库、万方数据知识服务平台、VIP中文科技期刊数据库、中国生物医学文献数据库、PubMed、Cochrane图书馆、Web of Science、Embase数据库等中英文数据库。检索时间为数据库建立至 2023 年 9 月。以肌肉骨骼疾病、康复治疗师为检索词,对研究的作者、发表时间、患病率和研究类型进行分析。采用美国医疗保健研究与质量机构(AHRQ)工具和纽卡斯尔-渥太华量表(NOS)评估文献质量。使用 Stata 15.0 软件进行荟萃分析。结果共纳入 28 篇文献,包括 8039 名康复治疗师。荟萃分析表明,康复治疗师的 WMSDs 总患病率为 72%(95%CI:64%-80%)。每年发病率最高的前三位是下背部(51%,95%CI:38%-64%)、颈部(42%,95%CI:30%-54%)和肩部(32%,95%CI:23%-41%)。每周发病率前三位分别是腰部(47%,95%CI:28%-65%)、颈部(35%,95%CI:14%-55%)和背部(30%,95%CI:17%-44%)。结论康复治疗师的 WMSDs 患病率较高,尤其是下背部、颈部、肩部和背部,这可能与长时间的弯腰驼背姿势以及上肢持续或反复用力有关。
{"title":"[A meta-analysis of the prevalence of work-related musculoskeletal disorders among rehabilitation therapists].","authors":"J Pan, M C Wang, S Y Zhao, Q Liu, H Y Zhai","doi":"10.3760/cma.j.cn121094-20231024-00093","DOIUrl":"10.3760/cma.j.cn121094-20231024-00093","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the prevalence of work-related musculoskeletal disorders (WMSDs) in rehabilitation therapists. <b>Methods:</b> In September 2023, the computer searched Chinese and English databases such as CNKI database, Wanfang Data Knowledge Service Platform, VIP Chinese Science and Technology Journal Database, Chinese Biomedical Literature Database, PubMed, Cochrane Library, Web of Science, Embase database, etc. The retrieval time was from the establishment of the database to September 2023. Musculoskeletal disorders, rehabilitation therapists were selected as search terms, and the author, publication time, prevalence rate, and study type were analyzed. The Agency for Healthcare Research and Quality (AHRQ) tool and The Newcastle-Ottawa Scale (NOS) were used to evaluate the quality of the literature. Stata 15.0 software was used for meta-analysis. <b>Results:</b> A total of 28 literatures were included, including 8039 rehabilitation therapists. Meta-analysis showed that the overall prevalence rate of WMSDs among rehabilitation therapists was 72% (95%<i>CI</i>: 64%-80%). The top 3 annual prevalence rates were lower back (51%, 95%<i>CI</i>: 38%-64%), neck (42%, 95%<i>CI</i>: 30%-54%) and shoulder (32%, 95%<i>CI</i>: 23%-41%). The top 3 weekly prevalence rates were lower back (47%, 95%<i>CI</i>: 28%-65%), neck (35%, 95%<i>CI</i>: 14%-55%) and back (30%, 95% <i>CI</i>: 17%-44%) . <b>Conclusion:</b> The higher prevalence of WMSDs in rehabilitation therapists, especially in the lower back, neck, shoulder and back, may be related to the prolonged stouching posture and the continuous or repeated exertion of the upper limbs.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"746-752"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Study on the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells and the intervention effect of N-acetylcysteine]. [超细炭黑诱导人支气管上皮细胞自噬和凋亡的分子机制及N-乙酰半胱氨酸的干预作用研究]。
Q3 Medicine Pub Date : 2024-09-20 DOI: 10.3760/cma.j.cn121094-20231010-00080
T Meng, H J Guo, Y Yao, Z H Mi, Y Tian, J Z Yu
<p><p><b>Objective:</b> To investigate the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells (BEAS-2B cells), and to study the intervention effect and mechanism of N-acetylcysteine (NAC) on ultrafine carbon black-induced oxidative damage in BEAS-2B cells. <b>Methods:</b> In March 2023, BEAS-2B cells were used as research object, an in vitro airway model exposed to ultrafine carbon black was constructed. A control group and three carbon black exposure groups (50, 100, 200 μg/ml) were set up, and the cells were treated with corresponding concentrations of ultrafine carbon black for 24 hours. In addition, the experiment was divided into control group, NAC+ control group, 100 μg/ml carbon black exposure group and NAC+ exposure group. The corresponding groups were treated with 2 mmol/L NAC for 1 h and 100 μg/ml ultrafine carbon black for 24 h, respectively. Cell viability was measured by CCK-8 assay. Intracellular reactive oxygen species (ROS) level was detected by chemical fluorescence method. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the content of malondialdehyde (MDA) were detected by colorimetry. The mRNA and protein expressions of autophagy-related genes[Atg5, Atg7, Beclin1, microtubule-associated protein light chain 3B (LC3B), p62 and lysosome-associated membrane protein 2 (LAMP2) ] and apoptosis-related genes [B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), Caspase3, Caspase9 and poly (ADP-ribose) polymerase 1 (PARP1) ] were determined by fluorescence quantitative PCR and Western blot. Cell apoptosis was determined by flow cytometry. <b>Results:</b> Compared with the control group, the relative survival rates of BEAS-2B cells in 50, 100, 200 μg/ml carbon black exposure groups were significantly decreased, the levels of ROS and MDA were significantly increased, and the activities of SOD, GSH-Px and CAT were significantly decreased (<i>P</i><0.05). The relative survival rate, ROS and MDA levels, SOD, GSH-Px and CAT activities were significantly correlated with the exposure dose of ultrafine carbon black (<i>r</i>(s)=-0.755, 0.826, 0.934, -0.810, -0.880, -0.840, <i>P</i><0.05). Compared with the control group, the relative expression levels of Atg5, Atg7, Beclin1, LC3B, p62, LAMP2, Bax, Caspase3, Caspase9, PARP1 mRNA and Atg5, Atg7, Beclin1, LC3BⅡ, p62, LAMP2, Bax, cleaved Caspase3 (C-Caspase3), cleaved Caspase9 (C-Caspase9), cleaved PARP1 (C-PARP1) protein and the ratio of LC3BⅡ/LC3BⅠ in 50, 100 and 200 μg/ml carbon black exposure groups were significantly increased, while the relative expression levels of Bcl-2 mRNA and protein were significantly decreased (<i>P</i><0.05). The changes of the above indexes were significantly correlated with the exposure dose of carbon black (<i>r</i>(s)=0.892, 0.879, 0.944, 0.892, 0.828, 0.880, 0.814, 0.794, 0.931, 0.918, 0.813, 0.866, 0.774, 0.695, 0.918, 0.761, 0.79
目的探讨超细炭黑诱导人支气管上皮细胞(BEAS-2B细胞)自噬和凋亡的分子机制,并研究N-乙酰半胱氨酸(NAC)对超细炭黑诱导BEAS-2B细胞氧化损伤的干预作用及机制。研究方法2023年3月,以BEAS-2B细胞为研究对象,构建暴露于超细炭黑的体外气道模型。设对照组和三个炭黑暴露组(50、100、200 μg/ml),分别用相应浓度的超细炭黑处理细胞 24 小时。此外,实验还分为对照组、NAC+ 对照组、100 μg/ml 炭黑暴露组和 NAC+ 暴露组。各组分别用 2 mmol/L NAC 处理 1 小时和 100 μg/ml 超细炭黑处理 24 小时。细胞活力用 CCK-8 法测定。细胞内活性氧(ROS)水平采用化学荧光法检测。用比色法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性以及丙二醛(MDA)的含量。自噬相关基因[Atg5、Atg7、Beclin1、微管相关蛋白轻链 3B(LC3B)、p62 和溶酶体相关膜蛋白 2(LAMP2)]和凋亡相关基因[B 细胞淋巴瘤 2(Bcl-2)、Bcl-2 相关 X(Bcl-2-associated X)、Bcl-2-associated X(Bcl-2-associated X)]的 mRNA 和蛋白表达量均有显著变化、Bcl-2-associated X protein (Bax), Caspase3, Caspase9 and poly (ADP-ribose) polymerase 1 (PARP1) ]和细胞凋亡相关基因。流式细胞术测定细胞凋亡。结果与对照组相比,50、100、200 μg/ml 炭黑暴露组 BEAS-2B 细胞的相对存活率显著降低,ROS 和 MDA 水平显著升高,SOD、GSH-Px 和 CAT 活性显著降低(Pr(s)=-0.755,0.826,0.934,-0.810,-0.880,-0.840,PPr(s)=0.892,0.879,0.944,0.892,0.828,0.880,0.814,0.794,0.931,0.918,0.813,0.866,0.774,0.695,0.918,0.761,0.794,0.944,0.833,0.866,0.905,-0.886,-0.748,PPPr(s)=0.944,PPConclusion:细胞自噬和凋亡可能是超细炭黑诱导 BEAS-2B 细胞氧化损伤的重要病理生理机制。NAC可通过调节氧化应激及自噬和凋亡的级联通路,减轻超细炭黑对BEAS-2B细胞的损伤。
{"title":"[Study on the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells and the intervention effect of N-acetylcysteine].","authors":"T Meng, H J Guo, Y Yao, Z H Mi, Y Tian, J Z Yu","doi":"10.3760/cma.j.cn121094-20231010-00080","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231010-00080","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells (BEAS-2B cells), and to study the intervention effect and mechanism of N-acetylcysteine (NAC) on ultrafine carbon black-induced oxidative damage in BEAS-2B cells. &lt;b&gt;Methods:&lt;/b&gt; In March 2023, BEAS-2B cells were used as research object, an in vitro airway model exposed to ultrafine carbon black was constructed. A control group and three carbon black exposure groups (50, 100, 200 μg/ml) were set up, and the cells were treated with corresponding concentrations of ultrafine carbon black for 24 hours. In addition, the experiment was divided into control group, NAC+ control group, 100 μg/ml carbon black exposure group and NAC+ exposure group. The corresponding groups were treated with 2 mmol/L NAC for 1 h and 100 μg/ml ultrafine carbon black for 24 h, respectively. Cell viability was measured by CCK-8 assay. Intracellular reactive oxygen species (ROS) level was detected by chemical fluorescence method. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the content of malondialdehyde (MDA) were detected by colorimetry. The mRNA and protein expressions of autophagy-related genes[Atg5, Atg7, Beclin1, microtubule-associated protein light chain 3B (LC3B), p62 and lysosome-associated membrane protein 2 (LAMP2) ] and apoptosis-related genes [B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), Caspase3, Caspase9 and poly (ADP-ribose) polymerase 1 (PARP1) ] were determined by fluorescence quantitative PCR and Western blot. Cell apoptosis was determined by flow cytometry. &lt;b&gt;Results:&lt;/b&gt; Compared with the control group, the relative survival rates of BEAS-2B cells in 50, 100, 200 μg/ml carbon black exposure groups were significantly decreased, the levels of ROS and MDA were significantly increased, and the activities of SOD, GSH-Px and CAT were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The relative survival rate, ROS and MDA levels, SOD, GSH-Px and CAT activities were significantly correlated with the exposure dose of ultrafine carbon black (&lt;i&gt;r&lt;/i&gt;(s)=-0.755, 0.826, 0.934, -0.810, -0.880, -0.840, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the control group, the relative expression levels of Atg5, Atg7, Beclin1, LC3B, p62, LAMP2, Bax, Caspase3, Caspase9, PARP1 mRNA and Atg5, Atg7, Beclin1, LC3BⅡ, p62, LAMP2, Bax, cleaved Caspase3 (C-Caspase3), cleaved Caspase9 (C-Caspase9), cleaved PARP1 (C-PARP1) protein and the ratio of LC3BⅡ/LC3BⅠ in 50, 100 and 200 μg/ml carbon black exposure groups were significantly increased, while the relative expression levels of Bcl-2 mRNA and protein were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The changes of the above indexes were significantly correlated with the exposure dose of carbon black (&lt;i&gt;r&lt;/i&gt;(s)=0.892, 0.879, 0.944, 0.892, 0.828, 0.880, 0.814, 0.794, 0.931, 0.918, 0.813, 0.866, 0.774, 0.695, 0.918, 0.761, 0.79","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 9","pages":"656-667"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Whole brain causal functional connectivity analysis of noise-induced deafness based on resting state-functional magnetic resonance imaging]. [基于静息态功能磁共振成像的噪声性耳聋的全脑因果功能连接分析]。
Q3 Medicine Pub Date : 2024-09-20 DOI: 10.3760/cma.j.cn121094-20231122-00126
A J Wang, R R Huang, C Y Wang, X R Ba, X H Bao, G W Zhang

Objective: To investigate the changes of directional connections of auditory and non-auditory in patients with noise-induced deafness (NID) by degree centrality (DC) and Granger causality analysis (GCA), and to explore the mode of brain function remodeling after NID. Methods: In October 2023, a total of 58 patients diagnosed with NID by the Occupational Diseases Department of Yantaishan Hospital of Yantai from 2014 to 2022 were collected as case group (NID group), and 42 healthy volunteers matched by gender, age and education level were selected as the control group (HC group). Resting state-functional magnetic resonance imaging (Rs-fMRI) was perfomed and PC analysis was performed. The brain regions with statistically significant differences in DC values between groups and the bilateral Heschl regions were extracted as regions of interest (ROI) for voxel-based whole brain GCA and correlation analysis. Results: Compared with HC group, the SOG.L DC value of NID group was lower, the connectivity values of SFGdor.L to SOG.L was increased, the connectivity value of PCL.L to SOG.L was decreased, the connectivity values of ORBmid.L, PCG.R and CUN. L/R to HES.L were increased, the connectivity value of SFGdor.L to HES.L was decreased, the connectivity value of HES.L to PCUN.L was decreased, the connectivity values of ORBsup.L and PCG.R to HES.R were increased, the connectivity value of HES.R to CUN.L was decreased (P voxel level<0.01, P cluster level<0.05). The connectivity value of PCL.L to SOG.L was negatively correlated with the weighted value of the better whisper frequency (P<0.05) . Conclusion: The NID patients have abnormal directional connectivity activity in multiple brain regions, such as auditory vision, executive control, somatosensory movement, and default mode network. It is suggested that hearing loss may cause complex neural remodeling between auditory and non-auditory centers.

目的通过度中心性(DC)和格兰杰因果关系分析(GCA)研究噪声性耳聋(NID)患者听觉和非听觉方向性连接的变化,并探讨NID后脑功能重塑的模式。研究方法2023年10月,收集2014-2022年烟台市烟台山医院职业病科确诊的NID患者共58例作为病例组(NID组),选择42名性别、年龄、文化程度相匹配的健康志愿者作为对照组(HC组)。进行静息状态功能磁共振成像(Rs-fMRI)和 PC 分析。提取组间 DC 值差异有统计学意义的脑区和双侧 Heschl 区作为感兴趣区(ROI),进行基于体素的全脑 GCA 和相关性分析。结果与 HC 组相比,NID 组 SOG.L DC 值降低,SFGdor.L 与 SOG.L 的连接值升高,PCL.L 与 SOG.L 的连接值降低,ORBmid.L、PCG.R 和 CUN.L/R 与 HES.L 的连接值降低。L/R到HES.L的连通性值升高,SFGdor.L到HES.L的连通性值降低,HES.L到PCUN.L的连通性值降低,ORBsup.L和PCG.R到HES.R的连通性值升高,HES.R到CUN.L的连通性值降低(P象素水平P簇水平P结论:NID患者在听觉视觉、执行控制、躯体感觉运动和默认模式网络等多个脑区存在异常的定向连接活动。这表明听力损失可能导致听觉中枢和非听觉中枢之间复杂的神经重塑。
{"title":"[Whole brain causal functional connectivity analysis of noise-induced deafness based on resting state-functional magnetic resonance imaging].","authors":"A J Wang, R R Huang, C Y Wang, X R Ba, X H Bao, G W Zhang","doi":"10.3760/cma.j.cn121094-20231122-00126","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231122-00126","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the changes of directional connections of auditory and non-auditory in patients with noise-induced deafness (NID) by degree centrality (DC) and Granger causality analysis (GCA), and to explore the mode of brain function remodeling after NID. <b>Methods:</b> In October 2023, a total of 58 patients diagnosed with NID by the Occupational Diseases Department of Yantaishan Hospital of Yantai from 2014 to 2022 were collected as case group (NID group), and 42 healthy volunteers matched by gender, age and education level were selected as the control group (HC group). Resting state-functional magnetic resonance imaging (Rs-fMRI) was perfomed and PC analysis was performed. The brain regions with statistically significant differences in DC values between groups and the bilateral Heschl regions were extracted as regions of interest (ROI) for voxel-based whole brain GCA and correlation analysis. <b>Results:</b> Compared with HC group, the SOG.L DC value of NID group was lower, the connectivity values of SFGdor.L to SOG.L was increased, the connectivity value of PCL.L to SOG.L was decreased, the connectivity values of ORBmid.L, PCG.R and CUN. L/R to HES.L were increased, the connectivity value of SFGdor.L to HES.L was decreased, the connectivity value of HES.L to PCUN.L was decreased, the connectivity values of ORBsup.L and PCG.R to HES.R were increased, the connectivity value of HES.R to CUN.L was decreased (<i>P</i> voxel level<0.01, <i>P</i> cluster level<0.05). The connectivity value of PCL.L to SOG.L was negatively correlated with the weighted value of the better whisper frequency (<i>P</i><0.05) . <b>Conclusion:</b> The NID patients have abnormal directional connectivity activity in multiple brain regions, such as auditory vision, executive control, somatosensory movement, and default mode network. It is suggested that hearing loss may cause complex neural remodeling between auditory and non-auditory centers.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 9","pages":"689-694"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Research on ameliorating pulmonary fibrosis in silicosis mice of Cordyceps cicadae polysaccharides]. [冬虫夏草多糖改善矽肺小鼠肺纤维化的研究]。
Q3 Medicine Pub Date : 2024-09-20 DOI: 10.3760/cma.j.cn121094-20230714-00250
L Cao, G L Wang, L Chen, S P Liu
<p><p><b>Objective:</b> A mouse silicosis model was constructed by injecting silicon dioxide (SiO(2)) particles into the trachea to explore the effect and mechanism of Cordyceps cicadae polysaccharides (CCP) on ameliorating pulmonary fibrosis in silicosis mice. <b>Methods:</b> In May 2023, CCP were extracted and isolated, the monosaccharide composition and functional group composition were analyzed by high performance liquid chromatography and Fourier transform infrared spectroscopy. C57BL/6J mice were injected with 50 μl 50 mg/ml SiO(2) suspension to construct silicosis mouse model, which were then randomly divided into model group, CCP intervention groups [low dose group (LCCP group), medium dose group (MCCP group) and high dose group (HCCP group) ], the control group was administered by physiological saline, 8 mice in each group. Mice in the CCP intervention groups received oral gavage administration once daily with CCP solution (100, 200 and 400 mg/kg), while control group and model group received physiological saline, lasted for 30 days. The body weight of mice was recorded and the lung coefficient was calculated. The pathomorphological changes of mouse lung tissue were determined by HE and Masson staining. The contents of fibrosis indexes [hydroxyproline acid (HYP), connective tissue growth factor (CTGF) and matrix metallopeptidase 2 (MMP-2) ] of lung tissue and the pro-inflammatory factors[tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) ] of lung tissue and alveolar lavage fluid were determined by ELISA. The expression level of Collagen Ⅰ was determined by immunohistochemistry. The relative protein expression levels of transforming growth factor-β1 (TGF-β1), P-Smad2, α-smooth muscle actin (α-SMA), Toll-like receptor 4 (TLR4), nuclear factor kappa-B p65 (NF-κBp65) and myeloid differentiation primary response gene 88 (MyD88) in lung tissue were determined by Western blot. <b>Results:</b> The total sugar content of the CCP was 86.78%, composed of D-mannose, D-rhamnose, D-glucose and D-galactose, with a molar ratio of 12.71∶1.53∶1.00∶12.64. The infrared spectrum indicated the characteristic groups of its polysaccharides. Compared with the control group, the body weight of mice in the model group was decreased, lung coefficient was increased, the contents of HYP, CTGF and MMP-2 in lung tissue were increased, and the contents of TNF-α, IL-1β and IL-6 in lung tissue and alveolar lavage fluid were increased (<i>P</i><0.05). The mice lung showed massive inflammatory cell infiltration and collagen fiber deposition, and the silicosis fibrosis was severe. The expression of CollagenⅠin lung tissue of model group was increased, and the proteins expression levels of TGF-β1, P-Smad2/Smad2, α-SMA, TLR4, NF-κBp65 and MyD88 were increased in mouse lung tissue (<i>P</i><0.05). Compared with the model group, the body weights of mice in the MCCP and HCCP groups were increased, the lung coefficients were decreased, the co
目的:通过向气管内注射二氧化硅(SiO(2))颗粒构建小鼠矽肺模型,探讨冬虫夏草多糖(CCP)对改善矽肺小鼠肺纤维化的作用和机制。研究方法2023年5月,提取并分离冬虫夏草多糖,采用高效液相色谱法和傅立叶变换红外光谱法分析其单糖组成和官能团组成。给C57BL/6J小鼠注射50 μl 50 mg/ml SiO(2)悬浮液,构建矽肺小鼠模型,然后随机分为模型组、CCP干预组[低剂量组(LCCP组)、中剂量组(MCCP组)和高剂量组(HCCP组)],对照组用生理盐水给药,每组8只。CCP 干预组小鼠每天一次口服 CCP 溶液(100、200 和 400 毫克/千克),对照组和模型组小鼠口服生理盐水,持续 30 天。记录小鼠体重并计算肺系数。通过 HE 和 Masson 染色测定小鼠肺组织的病理形态学变化。用酶联免疫吸附法测定肺组织纤维化指标[羟脯氨酸(HYP)、结缔组织生长因子(CTGF)和基质金属肽酶2(MMP-2)]和促炎因子[肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)]的含量。免疫组化法测定胶原蛋白Ⅰ的表达水平。通过 Western 印迹法测定肺组织中转化生长因子-β1(TGF-β1)、P-Smad2、α-平滑肌肌动蛋白(α-SMA)、Toll 样受体 4(TLR4)、核因子 kappa-B p65(NF-κBp65)和髓样分化初级反应基因 88(MyD88)的相对蛋白表达水平。结果CCP的总糖含量为86.78%,由D-甘露糖、D-鼠李糖、D-葡萄糖和D-半乳糖组成,摩尔比为12.71∶1.53∶1.00∶12.64。红外光谱显示了其多糖的特征基团。与对照组相比,模型组小鼠体重下降,肺系数升高,肺组织中 HYP、CTGF 和 MMP-2 含量升高,肺组织和肺泡灌洗液中 TNF-α、IL-1β 和 IL-6 含量升高(PPPPC结论:CCP 能降低小鼠肺组织中 HYP、CTGF 和 MMP-2 的含量:CCP可通过抑制TGF-β1/Smad通路和TLR4/核因子卡巴-B(NF-κB)通路的激活,降低肺组织中纤维化相关指标和促炎因子的水平,从而改善SiO(2)颗粒引起的小鼠肺部炎症和矽肺纤维化。
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引用次数: 0
[Research on the effect of training program based on service-oriented leadership theory on nurses' job burnout]. [基于服务型领导理论的培训项目对护士工作倦怠的影响研究]。
Q3 Medicine Pub Date : 2024-09-20 DOI: 10.3760/cma.j.cn121094-20240111-00011
Y X Du, K W Li

Objective: To explore the effect of training program based on service-oriented leadership theory on alleviating the degree of job burnout of clinical nurses, and provide reference for improving the situation of job burnout of nurses. Methods: From January to December 2023, 10 head nurses of a Grade A general hospital were selected by random number table method for training, and 5 nurses were selected from the department of each head nurse by random number table method, a total of 50 nurses were selected as research objects. Through the training program based on the service-oriented leadership theory, the selected head nurses were trained on the service-oriented leadership theory, job burnout and burnout mitigation methods and passed the examination. The training period was 2 months. The general data of 50 nurses were collected, and the job burnout of nurses was investigated by using the Maslach Burnout Inventory before and 6 months after the training of head nurses, and the changes of scores of each dimension were compared by paired t-test. Results: The nurses were (30.4±5.5) years old and their working life was (8.1±6.1) years. The total detection rate of job burnout before training was 100% (50/50), the detection rate of moderate to severe emotional exhaustion was 60% (30/50), and the detection rate after training was 36% (18/50). The detection rate of moderate to severe depersonalization was 72% (36/50) and 40% (20/50) after training. The detection rate of moderate to severe lack of job accomplishment was 86% (43/50) and 54% (27/50) after training. After 6 months of head nurses training, the scores of emotional exhaustion and depersonalization of nurses were significantly lower than those before training, while the scores of personal achievement were significantly higher than those before training, with statistical significances (P<0.05) . Conclusion: The training program based on service-oriented leadership theory can scientifically prevent and alleviate nurses' job burnout after receiving training of administrators, which is helpful to improve the current situation of nurses' job burnout and reduce the degree of nurses' job burnout, and can play a positive role in promoting nurses' physical and mental health.

目的探讨基于服务型领导理论的培训项目对缓解临床护士工作倦怠程度的影响,为改善护士工作倦怠状况提供参考。方法:2023年1月-12月,以随机数字表法抽取某甲级综合医院10名护士长作为培训对象,以随机数字表法从每位护士长所在科室抽取5名护士作为培训对象,共抽取50名护士作为研究对象。通过基于服务型领导理论的培训项目,对入选护士长进行服务型领导理论、工作倦怠及倦怠缓解方法的培训,并通过考核。培训期为 2 个月。收集了 50 名护士的一般资料,使用马斯拉赫职业倦怠量表调查了护士长培训前和培训后 6 个月的工作倦怠情况,并通过配对 t 检验比较了各维度得分的变化。结果显示护士年龄(30.4±5.5)岁,工作年限(8.1±6.1)年。培训前工作倦怠总检出率为 100%(50/50),中重度情绪衰竭检出率为 60%(30/50),培训后检出率为 36%(18/50)。中度至重度人格解体的检出率为 72%(36/50),训练后的检出率为 40%(20/50)。中度至重度缺乏工作成就感的检出率为 86%(43/50),培训后为 54%(27/50)。护士长培训 6 个月后,护士的情绪衰竭和人格解体得分明显低于培训前,而个人成就感得分明显高于培训前,差异有统计学意义(P结论:基于服务型领导理论的培训项目在接受管理者培训后,能科学预防和缓解护士的工作倦怠,有利于改善护士工作倦怠现状,降低护士工作倦怠程度,对促进护士身心健康起到积极作用。
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引用次数: 0
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中华劳动卫生职业病杂志
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