Pub Date : 2025-02-20DOI: 10.3760/cma.j.cn121094-20240119-00028
Y R Zhao, Y M Zheng, L Guan
Carpal tunnel syndrome is the most common peripheral nerve compression syndrome and a well-studied form of nerve entrapment syndrome as well. According to the etiology, the risk factors can be divided into individual risk factors and occupational risk factors. Individual risk factors include older age, gender (female), pregnancy, family history, hypothyroidism, diabetes, autoimmune diseases, advanced age, obesity, and wrist trauma, they are also associated with abnormal in the shapes and structures of the wist canal bones and tissues in individuals carpal tunnel bones and tissues. Occupational risk factors include the working posture of hands and wrists, work intensity, and tools, etc., that have attracted more and more attention from occupational disease prevention and control experts and management departments. In addition, the latest research also indicates that social psychosocial factors may also be an important factor in the occurrence and development of carpal tunnel syndrome. Therefore, it is necessary to actively carry out the prevention of occupational carpal tunnel syndrome, reduce the risk of the disease among workers and detect clinical symptoms as early as possible and intervene in time.
{"title":"[Advance on risk factors of occupational carpal tunnel syndrome].","authors":"Y R Zhao, Y M Zheng, L Guan","doi":"10.3760/cma.j.cn121094-20240119-00028","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240119-00028","url":null,"abstract":"<p><p>Carpal tunnel syndrome is the most common peripheral nerve compression syndrome and a well-studied form of nerve entrapment syndrome as well. According to the etiology, the risk factors can be divided into individual risk factors and occupational risk factors. Individual risk factors include older age, gender (female), pregnancy, family history, hypothyroidism, diabetes, autoimmune diseases, advanced age, obesity, and wrist trauma, they are also associated with abnormal in the shapes and structures of the wist canal bones and tissues in individuals carpal tunnel bones and tissues. Occupational risk factors include the working posture of hands and wrists, work intensity, and tools, etc., that have attracted more and more attention from occupational disease prevention and control experts and management departments. In addition, the latest research also indicates that social psychosocial factors may also be an important factor in the occurrence and development of carpal tunnel syndrome. Therefore, it is necessary to actively carry out the prevention of occupational carpal tunnel syndrome, reduce the risk of the disease among workers and detect clinical symptoms as early as possible and intervene in time.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 2","pages":"156-160"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-20DOI: 10.3760/cma.j.cn121094-20240109-00007
S L Yang, C X Liu, Y Ni, Y Liu, J M Zhang, Q Zeng
Objective: To analyze the differences in pediatric CT radiation dose and scanning parameter Settings between children's specialized hospitals and general hospitals, and to provide reference for the optimization strategy of pediatric CT radiation dose. Methods: From July to August 2022, a stratified sampling method was used to select 7 comprehensive hospitals and 2 children's hospitals in Tianjin, and on-site survey was conducted to collect the dose data of children's head, chest, and abdomen and pelvic CT examinations in these hospitals. The Wilcoxon rank sum test was used to analyze the differences in CTDI(vol) and DLP of the same age group and examination site among different types of hospitals, and the stepwise regression analysis method was used to analyze the effects of age, examination site, and scanning parameters on radiation dose CTDI(vol) and DLP. Results: The CTDI(vol) and DLP of head (1-<5, 5-<10, 10-<15 years old group), chest (5-<10, 10-<15 years old group) and abdomen (1-<5, 5-<10, 10-<15 years old group) in children's specialized hospitals were lower than those in general hospitals (head, 1-<5, 5-<10, 10-<15 years old group, CTDI(vol): Z=-9.32, -6.17, -9.86, P<0.001, DLP, Z=-4.41, -4.14, -5.76, P<0.001; chest, 5-<10, 10-<15 years old group, CTDI(vol): Z=-3.84, -5.35, P<0.001, DLP, Z=-3.59, -4.63, P<0.001; abdomen, 1-<5、5-<10 and 10-<15 years old group, CTDI(vol): Z=-7.88, -7.78, -8.53, P<0.001, DLP, Z=-7.24, -5.92, -3.44, P<0.001). The CT radiation dose in children was positively correlated with age, tube voltage, tube current time product and scan length, and negatively correlated with pitch and hospital type (general hospital and children's hospital) . Conclusion: There are some differences in the CT radiation dose and scanning parameters between children's hospitals and general hospitals. The results indicate that there is still much room for improvement in the optimization of radiation dose in pediatric CT examination.
{"title":"[Analysis of the difference in CT radiation dose between children's specialized hospitals and general hospitals].","authors":"S L Yang, C X Liu, Y Ni, Y Liu, J M Zhang, Q Zeng","doi":"10.3760/cma.j.cn121094-20240109-00007","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240109-00007","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the differences in pediatric CT radiation dose and scanning parameter Settings between children's specialized hospitals and general hospitals, and to provide reference for the optimization strategy of pediatric CT radiation dose. <b>Methods:</b> From July to August 2022, a stratified sampling method was used to select 7 comprehensive hospitals and 2 children's hospitals in Tianjin, and on-site survey was conducted to collect the dose data of children's head, chest, and abdomen and pelvic CT examinations in these hospitals. The Wilcoxon rank sum test was used to analyze the differences in CTDI(vol) and DLP of the same age group and examination site among different types of hospitals, and the stepwise regression analysis method was used to analyze the effects of age, examination site, and scanning parameters on radiation dose CTDI(vol) and DLP. <b>Results:</b> The CTDI(vol) and DLP of head (1-<5, 5-<10, 10-<15 years old group), chest (5-<10, 10-<15 years old group) and abdomen (1-<5, 5-<10, 10-<15 years old group) in children's specialized hospitals were lower than those in general hospitals (head, 1-<5, 5-<10, 10-<15 years old group, CTDI(vol): <i>Z</i>=-9.32, -6.17, -9.86, <i>P</i><0.001, DLP, <i>Z</i>=-4.41, -4.14, -5.76, <i>P</i><0.001; chest, 5-<10, 10-<15 years old group, CTDI(vol): <i>Z</i>=-3.84, -5.35, <i>P</i><0.001, DLP, <i>Z</i>=-3.59, -4.63, <i>P</i><0.001; abdomen, 1-<5、5-<10 and 10-<15 years old group, CTDI(vol): <i>Z</i>=-7.88, -7.78, -8.53, <i>P</i><0.001, DLP, <i>Z</i>=-7.24, -5.92, -3.44, <i>P</i><0.001). The CT radiation dose in children was positively correlated with age, tube voltage, tube current time product and scan length, and negatively correlated with pitch and hospital type (general hospital and children's hospital) . <b>Conclusion:</b> There are some differences in the CT radiation dose and scanning parameters between children's hospitals and general hospitals. The results indicate that there is still much room for improvement in the optimization of radiation dose in pediatric CT examination.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 2","pages":"117-122"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240306-00086
Y P Wang, L W Chen, G J Zhao, B Wu, M F Li, Z Q Lu
Chinorhodopyr is a widely used pesticide in agriculture, and the mortality rate of acute chinorhodopyr poisoning patients is high, with no effective treatment currently available. This paper summarized the clinical data of 8 patients with acute chlorfenapyr poisoning recently admitted to the First Affiliated Hospital of Wenzhou Medical University, including clinical manifestations, auxiliary examination and treatment process, so as to provide references for disease diagnosis and treatment, and improve clinicians' understanding of acute chlorfenapyr poisoning.
{"title":"[Analysis of 8 cases of acute chlorfenapyr poisoning].","authors":"Y P Wang, L W Chen, G J Zhao, B Wu, M F Li, Z Q Lu","doi":"10.3760/cma.j.cn121094-20240306-00086","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240306-00086","url":null,"abstract":"<p><p>Chinorhodopyr is a widely used pesticide in agriculture, and the mortality rate of acute chinorhodopyr poisoning patients is high, with no effective treatment currently available. This paper summarized the clinical data of 8 patients with acute chlorfenapyr poisoning recently admitted to the First Affiliated Hospital of Wenzhou Medical University, including clinical manifestations, auxiliary examination and treatment process, so as to provide references for disease diagnosis and treatment, and improve clinicians' understanding of acute chlorfenapyr poisoning.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"72-74"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240111-00010
Y Gong, Y K Wu, Y H Meng, X F Chen
Objective: To establish an inductively coupled plasma mass spectrometry (ICP-MS) method for the rapid determination of total bromine in whole blood, and to provide technical support for the monitoring of bromine exposure in occupational populations. Methods: In September 2023, 0.25 ml of whole blood sample was added with 0.25% tetramethylammonium hydroxide solution to a volume of 5.0 ml, ultrasonicated and homogenized, and then quantified by ICP-MS with rhodium solution as the online internal standard solution. Results: The linear relationship of bromine in blood was good within the range of 0~10.00 mg/L, with a correlation coefficient (r) >0.999. The limit of detection of the method was 0.07 mg/L, and the quantification limit was 0.22 mg/L. The recoveries of the total bromine in whole blood were in the range of 95.5%-102.9%, and the relative standard deviations (RSDs) were in the range of 3.1%-4.7% (n=7) . Conclusion: An ICP-MS method was established for the rapid determination of total bromine in whole blood, which is accurate, simple, rapid, highly automated, and can be used for the determination of total bromine in whole blood of occupationally exposed people.
{"title":"[Ultrasonic-assisted alkaline dilution-inductively coupled plasma mass spectrometry for the determination of total bromine in whole blood].","authors":"Y Gong, Y K Wu, Y H Meng, X F Chen","doi":"10.3760/cma.j.cn121094-20240111-00010","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240111-00010","url":null,"abstract":"<p><p><b>Objective:</b> To establish an inductively coupled plasma mass spectrometry (ICP-MS) method for the rapid determination of total bromine in whole blood, and to provide technical support for the monitoring of bromine exposure in occupational populations. <b>Methods:</b> In September 2023, 0.25 ml of whole blood sample was added with 0.25% tetramethylammonium hydroxide solution to a volume of 5.0 ml, ultrasonicated and homogenized, and then quantified by ICP-MS with rhodium solution as the online internal standard solution. <b>Results:</b> The linear relationship of bromine in blood was good within the range of 0~10.00 mg/L, with a correlation coefficient (<i>r</i>) >0.999. The limit of detection of the method was 0.07 mg/L, and the quantification limit was 0.22 mg/L. The recoveries of the total bromine in whole blood were in the range of 95.5%-102.9%, and the relative standard deviations (RSDs) were in the range of 3.1%-4.7% (<i>n</i>=7) . <b>Conclusion:</b> An ICP-MS method was established for the rapid determination of total bromine in whole blood, which is accurate, simple, rapid, highly automated, and can be used for the determination of total bromine in whole blood of occupationally exposed people.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"68-71"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240322-00111
T J Zhang, J Y Zhang
Acetamiprid and pyridaben is a compound pesticide, Due to its characteristics of high efficiency, and environmental safety, it has been widely used in agriculture in recent years.This article reports a case of poisoning from a mixture of pymetrozine and propargite pesticides. The patient experienced severe symptoms of poisoning such as vomiting, shortness of breath, abdominal discomfort, generalized cyanosis, bradycardia, and shock after self-administering the pesticide. After admission to the hospital, the patient was treated with atropine to increase heart rate, endotracheal intubation to assist breathing, and norepinephrine to maintain blood pressure. In addition, the patient received gastric lavage, catharsis, hemoperfusion, and continuous blood purification therapy to eliminate the toxins. The patient was successfully treated and discharged after recovery.
{"title":"[One case of successful treatment for acute poisoning of the pesticideAcetamiprid and pyridaben].","authors":"T J Zhang, J Y Zhang","doi":"10.3760/cma.j.cn121094-20240322-00111","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240322-00111","url":null,"abstract":"<p><p>Acetamiprid and pyridaben is a compound pesticide, Due to its characteristics of high efficiency, and environmental safety, it has been widely used in agriculture in recent years.This article reports a case of poisoning from a mixture of pymetrozine and propargite pesticides. The patient experienced severe symptoms of poisoning such as vomiting, shortness of breath, abdominal discomfort, generalized cyanosis, bradycardia, and shock after self-administering the pesticide. After admission to the hospital, the patient was treated with atropine to increase heart rate, endotracheal intubation to assist breathing, and norepinephrine to maintain blood pressure. In addition, the patient received gastric lavage, catharsis, hemoperfusion, and continuous blood purification therapy to eliminate the toxins. The patient was successfully treated and discharged after recovery.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"75-77"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240111-00013
M T Liu, Z Y B Fang, H L Zhao, Z Y Shi, R Hai, L Ning
Objective: To explore the risk factors of pneumoconiosis complicated with pulmonary tuberculosis, to construct a clinical prediction model for patients with pneumoconiosis complicated with pulmonary tuberculosis, and to provide a scientific basis for the prevention of pneumoconiosis complicated with pulmonary tuberculosis. Methods: In January 2024, a total of 232 patients with pneumoconiosis (including coal workers' pneumoconiosis and silicosis) who were treated in the Department of Respiratory and Critical Care Medicine of the Third People's Hospital of Xinjiang Uygur Autonomous Region (Xinjiang Uygur Autonomous Region Occupational Disease Hospital) from January 2022 to January 2023 were randomly selected as the study subjects. Collectted basic patient information and diagnostic data. Multivariate logistic regression analysis was used to screen the risk factors related to pneumoconiosis complicated with pulmonary tuberculosis. According to the results of multivariate logistic regression analysis, a nomogram was established, and the area under the receiver operating characteristic (ROC) curve (AUC), calibration curve and decision curve analysis (DCA) were used to evaluate the predictive ability. Results: Among the 232 patients with pneumoconiosis, 73 were complicated with pulmonary tuberculosis, accounting for 31.47% (73/232). Multivariate logistic regression analysis determined that dust exposure time, type of work, smoking history, and lung function level were all risk factors for pneumoconiosis complicated with tuberculosis (OR=10.33, 95%CI=1.92~55.66, OR=5.43, 95% CI=1.91~15.44, OR=3.10, 95% CI=1.15~8.37, OR=4.00, 95% CI=1.62~9.87; P<0.05). The constructed nomogram model has good clinical applicability when the area under the receiver operating characteristic (ROC) curve is 0.77 [95% CI (0.69, 0.73) ], the calibration curve is close to the ideal diagonal, the absolute error between the simulation curve and the actual curve is 0.03, and the DCA decision curve shows that the probability threshold of the nomogram model is 1%-90%. Conclusion: The risk of pneumoconiosis complicated with tuberculosis is high, and the risk factors of dust exposure time, smoking history, type of work and lung function level are high. This nomogram model can be used to predict the risk of pulmonary tuberculosis in patients with pneumoconiosis, which is helpful for early intervention.
{"title":"[Predictive risk analysis for pneumoconiosis combined with tuberculosis].","authors":"M T Liu, Z Y B Fang, H L Zhao, Z Y Shi, R Hai, L Ning","doi":"10.3760/cma.j.cn121094-20240111-00013","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240111-00013","url":null,"abstract":"<p><p><b>Objective:</b> To explore the risk factors of pneumoconiosis complicated with pulmonary tuberculosis, to construct a clinical prediction model for patients with pneumoconiosis complicated with pulmonary tuberculosis, and to provide a scientific basis for the prevention of pneumoconiosis complicated with pulmonary tuberculosis. <b>Methods:</b> In January 2024, a total of 232 patients with pneumoconiosis (including coal workers' pneumoconiosis and silicosis) who were treated in the Department of Respiratory and Critical Care Medicine of the Third People's Hospital of Xinjiang Uygur Autonomous Region (Xinjiang Uygur Autonomous Region Occupational Disease Hospital) from January 2022 to January 2023 were randomly selected as the study subjects. Collectted basic patient information and diagnostic data. Multivariate logistic regression analysis was used to screen the risk factors related to pneumoconiosis complicated with pulmonary tuberculosis. According to the results of multivariate logistic regression analysis, a nomogram was established, and the area under the receiver operating characteristic (ROC) curve (AUC), calibration curve and decision curve analysis (DCA) were used to evaluate the predictive ability. <b>Results:</b> Among the 232 patients with pneumoconiosis, 73 were complicated with pulmonary tuberculosis, accounting for 31.47% (73/232). Multivariate logistic regression analysis determined that dust exposure time, type of work, smoking history, and lung function level were all risk factors for pneumoconiosis complicated with tuberculosis (<i>OR</i>=10.33, 95%<i>CI</i>=1.92~55.66, <i>OR</i>=5.43, 95% <i>CI</i>=1.91~15.44, <i>OR</i>=3.10, 95% <i>CI</i>=1.15~8.37, <i>OR</i>=4.00, 95% <i>CI</i>=1.62~9.87; <i>P</i><0.05). The constructed nomogram model has good clinical applicability when the area under the receiver operating characteristic (ROC) curve is 0.77 [95% <i>CI</i> (0.69, 0.73) ], the calibration curve is close to the ideal diagonal, the absolute error between the simulation curve and the actual curve is 0.03, and the DCA decision curve shows that the probability threshold of the nomogram model is 1%-90%. <b>Conclusion:</b> The risk of pneumoconiosis complicated with tuberculosis is high, and the risk factors of dust exposure time, smoking history, type of work and lung function level are high. This nomogram model can be used to predict the risk of pulmonary tuberculosis in patients with pneumoconiosis, which is helpful for early intervention.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240117-00024
M F Sun, L Zhu, X Chen
<p><p><b>Objective:</b> To investigate the role of ferroptosis in renal cell injury induced by diquat (DQ) . <b>Methods:</b> From January to October 2022, human renal tubular epithelial (HK-2) cells were treated with DQ for 48 h, and different doses of ferroptosis inhibitors [deferoxamine (DFO), Fer-1] were added, and cells were harvested 24 h later. The experiment was divided into 6 groups (<i>n</i>=6) : control group, DQ group (60 μmol/L), 20 μmol/L DFO (DFO-H) group, 10 μmol/L DFO (DFO-L) group, 5 μmol/L Fer-1 (Fer-1-H) group, 0.5 μmol/L Fer-1 (Fer-1-L) group. From December 2022 to June 2023, male C57bl/6 mice were selected to establish the animal model, and the experimental group was divided into 4 groups (<i>n</i>=6) : control group, DQ group (25 mg/kg), DFO group (100 mg/kg) and Fer-1 group (2.5 μmol/kg). The changes of renal tissue were detected by HE staining. The fluorescence probe of ferrous ions was used to detect the change of iron ions in cells, and the colorimetric determination of total iron and ferrous ions in mouse kidney tissues was performed. Reverse transcription real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect mRNA and protein expression changes related to ferroptosis signaling pathway. TUNEL staining was used to detect apoptosis. Enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of antioxidant-related proteins and oxidative stress-related products. Differences among groups were analyzed by one-way analysis of variance. <b>Results:</b> In vitro test, compared with the control group, the iron ion level of HK-2 cells in DQ group was increased, the mRNA and protein expression levels of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and ferritin heavy chain (FTH) were decreased, the mRNA and protein expression levels of transferrin receptor 1 (TFR1) and divalent metal transporter 1 (DMT1) were increased, and the apoptosis level was significantly increased (<i>P</i><0.05). The expression levels of glutathione (GSH) and super oxide dismutase (SOD) in HK-2 cells in DQ group were significantly lower than those in control group (<i>P</i><0.05), and the expression levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly higher than those in control group (<i>P</i><0.05). Compared with DQ group, iron ion levels in HK-2 cells in the intervention groups of DFO and Fer-1 at different doses were decreased (<i>P</i><0.001), and GPX4, SLC7A11 and FTH mRNA and protein expression levels were increased, and the mRNA and protein expression levels of TFR1 and DMT1 were decreased in DFO-H and DFO-L groups (<i>P</i><0.05). The apoptosis levels in the intervention groups of DFO and Fer-1 at different doses were decreased compared with DQ group (<i>P</i><0.001), the expression levels of GSH and SOD were higher than those in DQ group (<i>P</i><0.05), and the expression levels of ROS were lower than those in DQ group (<i>P<
{"title":"[The role of ferroptosis in renal injury induced by diquat].","authors":"M F Sun, L Zhu, X Chen","doi":"10.3760/cma.j.cn121094-20240117-00024","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240117-00024","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the role of ferroptosis in renal cell injury induced by diquat (DQ) . <b>Methods:</b> From January to October 2022, human renal tubular epithelial (HK-2) cells were treated with DQ for 48 h, and different doses of ferroptosis inhibitors [deferoxamine (DFO), Fer-1] were added, and cells were harvested 24 h later. The experiment was divided into 6 groups (<i>n</i>=6) : control group, DQ group (60 μmol/L), 20 μmol/L DFO (DFO-H) group, 10 μmol/L DFO (DFO-L) group, 5 μmol/L Fer-1 (Fer-1-H) group, 0.5 μmol/L Fer-1 (Fer-1-L) group. From December 2022 to June 2023, male C57bl/6 mice were selected to establish the animal model, and the experimental group was divided into 4 groups (<i>n</i>=6) : control group, DQ group (25 mg/kg), DFO group (100 mg/kg) and Fer-1 group (2.5 μmol/kg). The changes of renal tissue were detected by HE staining. The fluorescence probe of ferrous ions was used to detect the change of iron ions in cells, and the colorimetric determination of total iron and ferrous ions in mouse kidney tissues was performed. Reverse transcription real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect mRNA and protein expression changes related to ferroptosis signaling pathway. TUNEL staining was used to detect apoptosis. Enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of antioxidant-related proteins and oxidative stress-related products. Differences among groups were analyzed by one-way analysis of variance. <b>Results:</b> In vitro test, compared with the control group, the iron ion level of HK-2 cells in DQ group was increased, the mRNA and protein expression levels of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and ferritin heavy chain (FTH) were decreased, the mRNA and protein expression levels of transferrin receptor 1 (TFR1) and divalent metal transporter 1 (DMT1) were increased, and the apoptosis level was significantly increased (<i>P</i><0.05). The expression levels of glutathione (GSH) and super oxide dismutase (SOD) in HK-2 cells in DQ group were significantly lower than those in control group (<i>P</i><0.05), and the expression levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly higher than those in control group (<i>P</i><0.05). Compared with DQ group, iron ion levels in HK-2 cells in the intervention groups of DFO and Fer-1 at different doses were decreased (<i>P</i><0.001), and GPX4, SLC7A11 and FTH mRNA and protein expression levels were increased, and the mRNA and protein expression levels of TFR1 and DMT1 were decreased in DFO-H and DFO-L groups (<i>P</i><0.05). The apoptosis levels in the intervention groups of DFO and Fer-1 at different doses were decreased compared with DQ group (<i>P</i><0.001), the expression levels of GSH and SOD were higher than those in DQ group (<i>P</i><0.05), and the expression levels of ROS were lower than those in DQ group (<i>P<","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"14-24"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240102-00001
Y M Zhang, F Y Jin, X M Gao, H Xu, Y Zhu, N Mao
<p><p><b>Objective:</b> To observe the effects of peptidylarginine deiminase 2 (PAD2) inhibitor AFM-30a on silicotic mice and its possible mechanisms. <b>Methods:</b> In May 2022, 40 SPF male C57BL/6J mice were randomly divided into control group, AFM-30a group, silicosis model group and AFM-30a treatment group, with 10 mice in each group. Silicosis model group and AFM-30a treatment group were perfused with silicon dioxide (SiO(2)) suspension (10 mg/piece, 50 μl), and the other groups were perfused with an equal amount of sodium chloride solution. After 2 weeks, AFM-30a group and AFM-30a treatment group were intraperitoneally injected AFM-30a (20 mg/kg, 100 μl) daily, and mice of other groups were injected with equal amounts of sodium chloride solution for 4 weeks. Mouse RAW264.7 monocytes/macrophages were cultured in vitro and divided into blank control group, AFM-30a group (5 μmol/L), SiO(2) group (200 μg/ml), and SiO(2)+AFM-30a group (200 μg/ml SiO(2) induction for 12 h, followed by 5 μmol/L AFM-30a treatment for 12 h). As well as blank control group, vimentin (Vim) group (2 μg/ml), citrullinated vimentin (Cit-Vim) group (2 μg/ml), and Cit-Vim+TLR4-C34 group (10 μmol/L TLR4-C34 treatment for 1 h, followed by 2 μg/ml Cit-Vim induction for 24 h). Hematoxylin Eosin (HE) and Masson staining were used to observe the pathological morphology of lung. The lung fieldclarity and lung texture of each group was observed by micro-CT. The number of positive cells was detected by tartrate resistant acid phosphatase (TRAP) staining. The localization and expression levels of PAD2, Cit-Vim, toll-like receptor 4 (TLR4) signaling and receptor activator of nuclear factor-κB ligand (RANKL) signaling proteins were measured by Immunofluorescence staining and Western blotting in vitro and in vivo. The experimental data were all presented as Mean±SD. A completely random design of one-way analysis of variance was used among the groups. The pduo comparison was performed using LSD test for homogeneity of variance and Tamhane's test for inconsistency. <b>Results:</b> Compared with the control group, the silicosis model group showed the formation of silicon nodules accompanied by collagen deposition, the silicosis model group showed thickened, and several high-density shadows of varying sizes in the lung field, and the number of TRAP positive cells in silicosis model group were increased significantly, the expression levels of PAD2, Cit-Vim, TLR4 and RANKL signal-related proteins were also significantly increased in silicosis groupmodel (<i>P</i><0.05). Compared with the silicosis model group, the AFM-30a treatment group reduced deposition of collagen in lung, and the number of TRAP positive cells was decreased in AFM-30a treatment group. The expression levels of PAD2, Cit-Vim, TLR4 and RANKL signaling related proteins were significantly decreased in AFM-30a treatment group (<i>P</i><0.05). In vitro, compared with the blank control group, the number of TRAP positive cells a
{"title":"[The effects and mechanisms of PAD2 inhibitor AFM-30a attenuates pulmonary fibrosis in silicotic mice].","authors":"Y M Zhang, F Y Jin, X M Gao, H Xu, Y Zhu, N Mao","doi":"10.3760/cma.j.cn121094-20240102-00001","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240102-00001","url":null,"abstract":"<p><p><b>Objective:</b> To observe the effects of peptidylarginine deiminase 2 (PAD2) inhibitor AFM-30a on silicotic mice and its possible mechanisms. <b>Methods:</b> In May 2022, 40 SPF male C57BL/6J mice were randomly divided into control group, AFM-30a group, silicosis model group and AFM-30a treatment group, with 10 mice in each group. Silicosis model group and AFM-30a treatment group were perfused with silicon dioxide (SiO(2)) suspension (10 mg/piece, 50 μl), and the other groups were perfused with an equal amount of sodium chloride solution. After 2 weeks, AFM-30a group and AFM-30a treatment group were intraperitoneally injected AFM-30a (20 mg/kg, 100 μl) daily, and mice of other groups were injected with equal amounts of sodium chloride solution for 4 weeks. Mouse RAW264.7 monocytes/macrophages were cultured in vitro and divided into blank control group, AFM-30a group (5 μmol/L), SiO(2) group (200 μg/ml), and SiO(2)+AFM-30a group (200 μg/ml SiO(2) induction for 12 h, followed by 5 μmol/L AFM-30a treatment for 12 h). As well as blank control group, vimentin (Vim) group (2 μg/ml), citrullinated vimentin (Cit-Vim) group (2 μg/ml), and Cit-Vim+TLR4-C34 group (10 μmol/L TLR4-C34 treatment for 1 h, followed by 2 μg/ml Cit-Vim induction for 24 h). Hematoxylin Eosin (HE) and Masson staining were used to observe the pathological morphology of lung. The lung fieldclarity and lung texture of each group was observed by micro-CT. The number of positive cells was detected by tartrate resistant acid phosphatase (TRAP) staining. The localization and expression levels of PAD2, Cit-Vim, toll-like receptor 4 (TLR4) signaling and receptor activator of nuclear factor-κB ligand (RANKL) signaling proteins were measured by Immunofluorescence staining and Western blotting in vitro and in vivo. The experimental data were all presented as Mean±SD. A completely random design of one-way analysis of variance was used among the groups. The pduo comparison was performed using LSD test for homogeneity of variance and Tamhane's test for inconsistency. <b>Results:</b> Compared with the control group, the silicosis model group showed the formation of silicon nodules accompanied by collagen deposition, the silicosis model group showed thickened, and several high-density shadows of varying sizes in the lung field, and the number of TRAP positive cells in silicosis model group were increased significantly, the expression levels of PAD2, Cit-Vim, TLR4 and RANKL signal-related proteins were also significantly increased in silicosis groupmodel (<i>P</i><0.05). Compared with the silicosis model group, the AFM-30a treatment group reduced deposition of collagen in lung, and the number of TRAP positive cells was decreased in AFM-30a treatment group. The expression levels of PAD2, Cit-Vim, TLR4 and RANKL signaling related proteins were significantly decreased in AFM-30a treatment group (<i>P</i><0.05). In vitro, compared with the blank control group, the number of TRAP positive cells a","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20240428-00191
Z X Li, Y Li, L Li, T Hou, X H Wu, Y H Chen, B Chen, Z L Shang
Objective: To investigatethe effects of different blood lead levels on indicators of immune function in occupationally lead-exposed populations. Methods: From October to December 2023, a total of 126 occupationally exposed lead workers of a company in Guizhou Province were selected, and their basic information was collected through questionnaires. Inductively coupled plasma mass spectrometry was used to detect blood lead levels in the study population. Workers were categorized into Group 1, Group 2 and Group 3 based on blood lead levels (blood lead levels <200 μg/L, 200~400 μg/L and >400 μg/L). Lymphocyte subpopulation marker leukocyte differentiation antigen (CD) in peripheral blood and interleukin (IL) -1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17, tumor necrosis factor-alpha (TNF-α), and interferon (IFN) -γ in serum were examined by flow cytometry. Serum levels of immunoglobulin (Ig) A, IgG, IgM, and complement proteins (C3, C4) were measured by immunoscattering turbidimetry. Data were statistically analyzed using rank sum test, Chi-square test or Fisher exact probability method for multiple samples. Results: Compared with group 1, the percentage of CD3(+)CD8(+) and CD4(+)CD25(+) cells decreased (P<0.05) and the percentage of CD4(+) CD95(+) cells increased in the lead-exposed populations in groups 2 and 3 (P<0.05) ; however, the serum IL-1β, IL-2, IL- 5, IL-8, IL-12p70, and IFN-γ levels were decreased (P<0.05) in group 3. Meanwhile, IgG (P<0.05) and IgM levels in serum of lead-exposed population in group 2 and group 3 were reduced (P<0.05) comparing with group 1. Spearman correlation analysis showed that blood lead levels of workers were negatively correlated with the percentage of peripheral blood CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (r(s)=-0.20, -0.22, -0.23, -0.24, -0.26, -0.35, P<0.05), but was positively correlated with the level of CD3(+)CD4(+) cells (r(s)= 0.18, P<0.05). Regression analysis revealed that blood lead level was a risk factor for the percentage of CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (P<0.05) . Conclusion: Different lead loads can lead to abnormal changes in peripheral blood lymphocyte subsets, cytokines, and immunoglobulin levels in occupationally lead-exposed people. Lead exposure in occupationally lead-exposed populations may affect their immune function.
{"title":"[Effects of occupational lead exposure on the indicators of immune function of workers].","authors":"Z X Li, Y Li, L Li, T Hou, X H Wu, Y H Chen, B Chen, Z L Shang","doi":"10.3760/cma.j.cn121094-20240428-00191","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240428-00191","url":null,"abstract":"<p><p><b>Objective:</b> To investigatethe effects of different blood lead levels on indicators of immune function in occupationally lead-exposed populations. <b>Methods:</b> From October to December 2023, a total of 126 occupationally exposed lead workers of a company in Guizhou Province were selected, and their basic information was collected through questionnaires. Inductively coupled plasma mass spectrometry was used to detect blood lead levels in the study population. Workers were categorized into Group 1, Group 2 and Group 3 based on blood lead levels (blood lead levels <200 μg/L, 200~400 μg/L and >400 μg/L). Lymphocyte subpopulation marker leukocyte differentiation antigen (CD) in peripheral blood and interleukin (IL) -1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17, tumor necrosis factor-alpha (TNF-α), and interferon (IFN) -γ in serum were examined by flow cytometry. Serum levels of immunoglobulin (Ig) A, IgG, IgM, and complement proteins (C3, C4) were measured by immunoscattering turbidimetry. Data were statistically analyzed using rank sum test, Chi-square test or Fisher exact probability method for multiple samples. <b>Results:</b> Compared with group 1, the percentage of CD3(+)CD8(+) and CD4(+)CD25(+) cells decreased (<i>P</i><0.05) and the percentage of CD4(+) CD95(+) cells increased in the lead-exposed populations in groups 2 and 3 (<i>P</i><0.05) ; however, the serum IL-1β, IL-2, IL- 5, IL-8, IL-12p70, and IFN-γ levels were decreased (<i>P</i><0.05) in group 3. Meanwhile, IgG (<i>P</i><0.05) and IgM levels in serum of lead-exposed population in group 2 and group 3 were reduced (<i>P</i><0.05) comparing with group 1. Spearman correlation analysis showed that blood lead levels of workers were negatively correlated with the percentage of peripheral blood CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (<i>r</i>(s)=-0.20, -0.22, -0.23, -0.24, -0.26, -0.35, <i>P</i><0.05), but was positively correlated with the level of CD3(+)CD4(+) cells (<i>r</i>(s)= 0.18, <i>P</i><0.05). Regression analysis revealed that blood lead level was a risk factor for the percentage of CD8(+)CD95(+) cells, CD3(+)CD8(+) cells, CD3(-)CD16(+) CD56(+) cells, IgG, IgM, and IgA (<i>P</i><0.05) . <b>Conclusion:</b> Different lead loads can lead to abnormal changes in peripheral blood lymphocyte subsets, cytokines, and immunoglobulin levels in occupationally lead-exposed people. Lead exposure in occupationally lead-exposed populations may affect their immune function.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.3760/cma.j.cn121094-20231114-00113
L Wang, H Fan, X J Wang, X X Xie, Z F Liu
Objective: To investigate the current situation of uncivilized behavior in workplace encountered by nurses in Xianyang City, and analyze its influencing factors, so as to provide a basis for formulating corresponding intervention measures. Methods: In May 2023, convenience sampling was used to investigate the general information, current situation of uncivilized behavior in workplace and coping style of 1310 nurses in 8 secondary and tertiary general hospitals in Xianyang City by using the general information questionnaire, the Chinese version of the Nurse Incivility Scale and the Simplified Coping Style Questionnaire. Multiple linear regression analysis was used to analyze the influencing factors of uncivilized behavior encountered by nurses. Results: A total of 1285 valid questionnaires were collected, with an effective recovery rate of 98.09%. The total score of uncivilized behavior encountered by nurses was (86.41±22.49) points. Among them, the scores of five dimensions were nurses' uncivilized behavior (19.95±4.70) points, patients' uncivilized behavior (23.66±8.96) points, supervisors' uncivilized behavior (13.25±4.87) points, doctors' uncivilized behavior (14.32±5.63) points and colleagues' uncivilized behavior (15.23±4.93) points. The multiple linear regression analysis results showed that the hospital level and coping style were the significant influencing factors of uncivilized behavior in workplace encountered by nurses (β=0.104, 0.056; P<0.05) . Conclusion: The current situation of uncivilized behavior in workplace encountered by nurses needs to be improved. Hospital managers should focus on coping styles, such as setting up simulation scene exercises, team cooperation training, training and counseling, so as to reduce the uncivilized behavior in workplace encountered by nurses.
{"title":"[Study on the current situation and influencing factors of uncivilized behavior in workplace encountered by nurses in Xianyang City].","authors":"L Wang, H Fan, X J Wang, X X Xie, Z F Liu","doi":"10.3760/cma.j.cn121094-20231114-00113","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231114-00113","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the current situation of uncivilized behavior in workplace encountered by nurses in Xianyang City, and analyze its influencing factors, so as to provide a basis for formulating corresponding intervention measures. <b>Methods:</b> In May 2023, convenience sampling was used to investigate the general information, current situation of uncivilized behavior in workplace and coping style of 1310 nurses in 8 secondary and tertiary general hospitals in Xianyang City by using the general information questionnaire, the Chinese version of the Nurse Incivility Scale and the Simplified Coping Style Questionnaire. Multiple linear regression analysis was used to analyze the influencing factors of uncivilized behavior encountered by nurses. <b>Results:</b> A total of 1285 valid questionnaires were collected, with an effective recovery rate of 98.09%. The total score of uncivilized behavior encountered by nurses was (86.41±22.49) points. Among them, the scores of five dimensions were nurses' uncivilized behavior (19.95±4.70) points, patients' uncivilized behavior (23.66±8.96) points, supervisors' uncivilized behavior (13.25±4.87) points, doctors' uncivilized behavior (14.32±5.63) points and colleagues' uncivilized behavior (15.23±4.93) points. The multiple linear regression analysis results showed that the hospital level and coping style were the significant influencing factors of uncivilized behavior in workplace encountered by nurses (<i>β</i>=0.104, 0.056; <i>P</i><0.05) . <b>Conclusion:</b> The current situation of uncivilized behavior in workplace encountered by nurses needs to be improved. Hospital managers should focus on coping styles, such as setting up simulation scene exercises, team cooperation training, training and counseling, so as to reduce the uncivilized behavior in workplace encountered by nurses.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"43 1","pages":"58-61"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}