Pub Date : 2018-12-28DOI: 10.36233/0372-9311-2018-6-110-114
Е. П. Анохина, М. М. Исува, С. В. Старцева, Е. А. Мотина, Н. А. Михайлова, Ольга Сергеевна Корнеева
Aim. Investigation of the effect of fucose in the diet on the gastrointestinal microflora of experimental animals with experimental dysbiosis, the humoral factors of nonspecific immunity, as well as the degree of fucosylation of oocytes and the proportion of oocytes that can be fertilized. Materials and methods. Prebiotic properties of fucose were studied by analyzing the luminal microflora of experimental mice against the background of experimental dysbiosis. Investigation of factors of nonspecific immunity was carried out after immunization of mice according to the level of antibody formation in blood serum by the method of enzyme immunoassay. The degree of fucosylation of oocytes was assessed by the intensity of their luminescence upon microscopy of oocytes of experimental mice on a fluorescent microscope. Results. The use of fucose in all tested doses led to the restoration of the composition and quantity of the gastrointestinal microflora. For the correction of dysbiosis, the optimal concentration of fucose was 0.02% of the body weight of the experimental animals. Inclusion of fucose in a diet of experimental animals in the amount of 0.008% to the body weight provided the highest level of immune response. The degree of fucosylation of oocytes, the proportion of oocytes capable of fertilization was increased when fucose were introduced in the amount of 0.008% to the body weight of the mice. Conclusion. Bifidogenic and lactogenic activity of fucose is established. The ability of fucose to stimulate an increase in the level of antibodies in in blood serum is shown. The tendency of positive effect of fucose in the diet of mice on the degree of fucosylation of oocytes was revealed.
{"title":"ИССЛЕДОВАНИЕ ПРЕБИОТИЧЕСКИХ, ИММУНОСТИМУЛИРУЮЩИХ СВОЙСТВ ФУКОЗЫ И ЕЕ ВЛИЯНИЯ НА РЕПРОДУКТИВНУЮ ФУНКЦИЮ","authors":"Е. П. Анохина, М. М. Исува, С. В. Старцева, Е. А. Мотина, Н. А. Михайлова, Ольга Сергеевна Корнеева","doi":"10.36233/0372-9311-2018-6-110-114","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-110-114","url":null,"abstract":"Aim. Investigation of the effect of fucose in the diet on the gastrointestinal microflora of experimental animals with experimental dysbiosis, the humoral factors of nonspecific immunity, as well as the degree of fucosylation of oocytes and the proportion of oocytes that can be fertilized. Materials and methods. Prebiotic properties of fucose were studied by analyzing the luminal microflora of experimental mice against the background of experimental dysbiosis. Investigation of factors of nonspecific immunity was carried out after immunization of mice according to the level of antibody formation in blood serum by the method of enzyme immunoassay. The degree of fucosylation of oocytes was assessed by the intensity of their luminescence upon microscopy of oocytes of experimental mice on a fluorescent microscope. Results. The use of fucose in all tested doses led to the restoration of the composition and quantity of the gastrointestinal microflora. For the correction of dysbiosis, the optimal concentration of fucose was 0.02% of the body weight of the experimental animals. Inclusion of fucose in a diet of experimental animals in the amount of 0.008% to the body weight provided the highest level of immune response. The degree of fucosylation of oocytes, the proportion of oocytes capable of fertilization was increased when fucose were introduced in the amount of 0.008% to the body weight of the mice. Conclusion. Bifidogenic and lactogenic activity of fucose is established. The ability of fucose to stimulate an increase in the level of antibodies in in blood serum is shown. The tendency of positive effect of fucose in the diet of mice on the degree of fucosylation of oocytes was revealed.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88389122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-28DOI: 10.36233/0372-9311-2018-4-119-124
I. Tartakovsky, O. Gruzdeva, T. Karpova, Y. Dronina, T. Tarasova, O. G. Loginova, M. Dmitrieva
Цель. Сравнительное изучение различных методических подходов, направленных на элиминацию легионелл в потенциально опасных водных системах. Материалы и методы. Оценку эффективности краткосрочного повышения температуры воды до 70С проводили в модуле водоснабжения с регулируемым температурным режимом объемом 290 литров. Оценку эффективности метода каталитической очистки воды проводили в бассейне для водоплавающей птицы объемом 10 000 литров. Исходный, промежуточный и конечный уровень колонизации водных систем Legionella pneumophila проводили в соответствии с методикой, описанной в МУК 4.2.2217-07. Наличие биопленок, содержащих легионеллы, на поверхности оборудования и границе водной среды или в шаровых кранах системы водоснабжения определяли визуально с последующим бактериологическим подтверждением присутствия легионелл в биопленках. Результаты Показана высокая бактерицидная активность применявшихся методов в отношении планктонных форм и биопленок легионелл в водных системах различного типа. Полученные результаты свидетельствуют, что тепловой шок (70С в течение 24 часов) в условиях работающего модуля системы горячего водоснабжения обладает краткосрочным эффектом, не превышающим 2 месяцев эксплуатации. Бактерицидный эффект каталитической очистки воды при достаточно высоком исходном уровне контаминации водного объекта проявлялся не ранее 3 недель экспозиции и сохранялся в течение 2 месяцев эксплуатации каталитического модуля. Заключение. Представленные в работе методические подходы могут быть использованы для разработки эффективной и рациональной стратегии обеспечения профилактики легионеллеза при эксплуатации различных водных систем и объектов в общественных зданиях.
{"title":"ANALYSIS OF THE DIFFERENT METHODICAL APPROACHES DIRECTED ON THE ELIMINATION OF PLANKTON FORMS AND LEGIONELLA BIOFILMS FROM POTENTIALLY DANGEROUS WATER SYSTEMS","authors":"I. Tartakovsky, O. Gruzdeva, T. Karpova, Y. Dronina, T. Tarasova, O. G. Loginova, M. Dmitrieva","doi":"10.36233/0372-9311-2018-4-119-124","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-4-119-124","url":null,"abstract":"Цель. Сравнительное изучение различных методических подходов, направленных на элиминацию легионелл в потенциально опасных водных системах. Материалы и методы. Оценку эффективности краткосрочного повышения температуры воды до 70С проводили в модуле водоснабжения с регулируемым температурным режимом объемом 290 литров. Оценку эффективности метода каталитической очистки воды проводили в бассейне для водоплавающей птицы объемом 10 000 литров. Исходный, промежуточный и конечный уровень колонизации водных систем Legionella pneumophila проводили в соответствии с методикой, описанной в МУК 4.2.2217-07. Наличие биопленок, содержащих легионеллы, на поверхности оборудования и границе водной среды или в шаровых кранах системы водоснабжения определяли визуально с последующим бактериологическим подтверждением присутствия легионелл в биопленках. Результаты Показана высокая бактерицидная активность применявшихся методов в отношении планктонных форм и биопленок легионелл в водных системах различного типа. Полученные результаты свидетельствуют, что тепловой шок (70С в течение 24 часов) в условиях работающего модуля системы горячего водоснабжения обладает краткосрочным эффектом, не превышающим 2 месяцев эксплуатации. Бактерицидный эффект каталитической очистки воды при достаточно высоком исходном уровне контаминации водного объекта проявлялся не ранее 3 недель экспозиции и сохранялся в течение 2 месяцев эксплуатации каталитического модуля. Заключение. Представленные в работе методические подходы могут быть использованы для разработки эффективной и рациональной стратегии обеспечения профилактики легионеллеза при эксплуатации различных водных систем и объектов в общественных зданиях.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91127902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-38-44
E. A. Prokopeva, K. A. Sharshov, Anna Romanovskaya, I. Sobolev, Olga Kurskaya, E. I. Soloveva, Lidiya V. Shestopalova, A. V. Zaykovskaya, A. Alekseev, A. M. Shestopalov
Aim. Compare the degree of pathogenicity of newly emerging influenza virus strains with pandemic potential by experimental infection of BALB/c mice with highly pathogenic avian influenza A(H5N1) virus and mouse adapted pandemic influenza A(H1N1)pdm09 virus. Materials and methods. The first group of BALB/c mice (n=24) were infected with highly pathogenic avian influenza A(H5N1) virus in a dose of 5 LD50, and the second group (n=24) were infected with mouse adapted pandemic influenza A(H1N1)pdm09 virus at a dose of 5 LD50. Determination of LD50 and TCID50 were performed by virological methods. Morphological changes in internal organs (lung, brain, liver, kidney, spleen) were examined by light and transmission electron microscopy. Results. Virologic analysis showed that both strains are highly lethal for mice. Microscopic examination revealed the development of interstitial pneumonia in the lungs and generalization of infection in the internal organs. Conclusion. In both groups of BALB/c mice experimentally infected with highly pathogenic avian influenza A(H5N1) and mouse adapted pandemic influenza A(H1N1)pdm09 virus were revealed the development of a highly lethal disease in the form of respiratory pneumonia. It is noted, different mechanisms of pathological process: in case of infection with mouse adapted pandemic influenza A(H1N1)pdm09 virus firstly developed bronchitis, which quickly exacerbated by the development of alveolitis, while in case of infection with highly pathogenic avian influenza A(H5N1) immediately developed alveolitis. On the 6th day after infection recorded the development of generalized infection in mice of both experimental groups.
{"title":"СРАВНИТЕЛЬНЫЙ АНАЛИЗ ПАТОГЕННОСТИ ВИРУСОВ ГРИППА A(H5N1) И A(H1N1)PDM09 У ЛАБОРАТОРНЫХ МЫШЕЙ","authors":"E. A. Prokopeva, K. A. Sharshov, Anna Romanovskaya, I. Sobolev, Olga Kurskaya, E. I. Soloveva, Lidiya V. Shestopalova, A. V. Zaykovskaya, A. Alekseev, A. M. Shestopalov","doi":"10.36233/0372-9311-2018-2-38-44","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-38-44","url":null,"abstract":"Aim. Compare the degree of pathogenicity of newly emerging influenza virus strains with pandemic potential by experimental infection of BALB/c mice with highly pathogenic avian influenza A(H5N1) virus and mouse adapted pandemic influenza A(H1N1)pdm09 virus. Materials and methods. The first group of BALB/c mice (n=24) were infected with highly pathogenic avian influenza A(H5N1) virus in a dose of 5 LD50, and the second group (n=24) were infected with mouse adapted pandemic influenza A(H1N1)pdm09 virus at a dose of 5 LD50. Determination of LD50 and TCID50 were performed by virological methods. Morphological changes in internal organs (lung, brain, liver, kidney, spleen) were examined by light and transmission electron microscopy. Results. Virologic analysis showed that both strains are highly lethal for mice. Microscopic examination revealed the development of interstitial pneumonia in the lungs and generalization of infection in the internal organs. Conclusion. In both groups of BALB/c mice experimentally infected with highly pathogenic avian influenza A(H5N1) and mouse adapted pandemic influenza A(H1N1)pdm09 virus were revealed the development of a highly lethal disease in the form of respiratory pneumonia. It is noted, different mechanisms of pathological process: in case of infection with mouse adapted pandemic influenza A(H1N1)pdm09 virus firstly developed bronchitis, which quickly exacerbated by the development of alveolitis, while in case of infection with highly pathogenic avian influenza A(H5N1) immediately developed alveolitis. On the 6th day after infection recorded the development of generalized infection in mice of both experimental groups.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78183175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-87-94
Т. А. Семененко, Василий Акимкин
Seroepidemiology is a potentially powerful tool for predicting and monitoring the effectiveness of specific prevention program using studies of antibody prevalence. The availability of a certified collection of blood serum (serum bank) allows to carry out a reliable assessment of population immunity to vaccine-preventable diseases; to determine the degree of epidemiological risk of the infection spread in various areas of the country; to implement short-term and long-term forecasting of changes in the situation on topical infections; to substantiate preventive measures in the system of biosafety for defined population groups and decreed contingents; to provide information necessary for making the optimal management decisions.
{"title":"СЕРОЭПИДЕМИОЛОГИЧЕСКИЕ ИССЛЕДОВАНИЯ В СИСТЕМЕ НАДЗОРА ЗА ВАКЦИНОУПРАВЛЯЕМЫМИ ИНФЕКЦИЯМИ","authors":"Т. А. Семененко, Василий Акимкин","doi":"10.36233/0372-9311-2018-2-87-94","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-87-94","url":null,"abstract":"Seroepidemiology is a potentially powerful tool for predicting and monitoring the effectiveness of specific prevention program using studies of antibody prevalence. The availability of a certified collection of blood serum (serum bank) allows to carry out a reliable assessment of population immunity to vaccine-preventable diseases; to determine the degree of epidemiological risk of the infection spread in various areas of the country; to implement short-term and long-term forecasting of changes in the situation on topical infections; to substantiate preventive measures in the system of biosafety for defined population groups and decreed contingents; to provide information necessary for making the optimal management decisions.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86408016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-10-15
E. Kalinichenko, S. Skhodova, N. Akhmatova, N. Mikhailova
Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on the phagocytic and bactericidal activity of immunocompetent cells in mice. Materials and methods. Preparation: 25 pg of OprF, 50 pg of aTox sorbed by 75 pg of aluminum hydroxide. For immunization, the recombinant protein preparation was mixed in equal weight fractions with an aluminum hydroxide gel, diluted in phosphate buffered saline, and sorbed for 12 hours at 4°C. The vaccine preparation was administered intraperitoneally in 0.5 ml to BALB/с mice. The phagocytic activity of peripheral blood leukocytes was determined from the absorption capacity of heat-killed FITC-labeled Staphylococcus aureus by neutrophils and monocytes of immunized mice by flow cytometry. The bactericidal activity of mice blood leukocytes was assessed for the live culture of S.aureus using flow cytometry Cytomix FC-500 (Beckman Coulter). Results. Administration to mice of recombinant proteins P. aeruginosa OprF and aTox sorbed on aluminum hydroxide led to an increase in the phagocytic and bactericidal activity of monocytes and granulocytes of peripheral blood. The maximum increase in the number of phagocytized monocytes was observed on the 7th, and granulocytes on the 17th day after the first immunization. Booster immunization did not lead to additional stimulation of phagocytic activity, but the number of phagocytic cells was significantly (p<0.05) higher than control (intact mice). Conclusion. Candidate vaccine against P. aeruginosa based on its recombinant proteins OprF and aTox activates the cellular unit of the immune system with the induction of the activity of professional macrophages.
{"title":"IMMUNIZATION WITH PROTEINS OF PSEUDOMONAS AERUGINOSA OprF AND aTox ENHANCES THE PHAGOCYTIC AND BACTERICIDAL ACTIVITY OF LEUKOCYTES IN MICE","authors":"E. Kalinichenko, S. Skhodova, N. Akhmatova, N. Mikhailova","doi":"10.36233/0372-9311-2018-2-10-15","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-10-15","url":null,"abstract":"Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on the phagocytic and bactericidal activity of immunocompetent cells in mice. Materials and methods. Preparation: 25 pg of OprF, 50 pg of aTox sorbed by 75 pg of aluminum hydroxide. For immunization, the recombinant protein preparation was mixed in equal weight fractions with an aluminum hydroxide gel, diluted in phosphate buffered saline, and sorbed for 12 hours at 4°C. The vaccine preparation was administered intraperitoneally in 0.5 ml to BALB/с mice. The phagocytic activity of peripheral blood leukocytes was determined from the absorption capacity of heat-killed FITC-labeled Staphylococcus aureus by neutrophils and monocytes of immunized mice by flow cytometry. The bactericidal activity of mice blood leukocytes was assessed for the live culture of S.aureus using flow cytometry Cytomix FC-500 (Beckman Coulter). Results. Administration to mice of recombinant proteins P. aeruginosa OprF and aTox sorbed on aluminum hydroxide led to an increase in the phagocytic and bactericidal activity of monocytes and granulocytes of peripheral blood. The maximum increase in the number of phagocytized monocytes was observed on the 7th, and granulocytes on the 17th day after the first immunization. Booster immunization did not lead to additional stimulation of phagocytic activity, but the number of phagocytic cells was significantly (p<0.05) higher than control (intact mice). Conclusion. Candidate vaccine against P. aeruginosa based on its recombinant proteins OprF and aTox activates the cellular unit of the immune system with the induction of the activity of professional macrophages.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75642434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-3-9
С. Б. Чекнев, Е. И. Вострова, С. В. Кисиль, М. А. Сарычева, А. В. Востров
Aim. The work was performed with the purpose to study an inhibitory action of millimolar concentrations of divalent metal ions, which differ by primary mechanisms of their toxicity, on the culture of S.pyogenes. Materials and methods. Suspensions of S.pyogenes bacteria which contained 10 8 CFU/ml were sown by the lawns into the standard Petri dishes coated with the supplemented Nutrient Agar. 30 min later the salt solutions of divalent metals were added by the 5 pl drops on the surfaces of the lawns with use of 36-channel stamp replicator. The salt solutions contained the metals tested at the concentrations ranged between 5x10 -3 M to 5x10 -1 M. Then the dishes with bacterial cultures were incubated for 24 hrs at 37°C followed by measuring diameter of the area of culture growth inhibition. The probes of material obtained from the centers of the stunting areas were passed into the centrifuge tubes with the supplemented Nutrient Broth, incubated for up to five days at 37°C and tested for the Broth clarity. Results. In presence of the metal concentrations ranged between 50 to 500 mM inhibitory action towards S.pyogenes bacteria was registered as relatively low due to the effects of copper or ferrous ions, as intermediate due to the presence of cobalt, nickel or manganese, and as high due to the effects of zinc ions. At the same time ferrous or copper ions demonstrated high bactericidal activity, zinc ions showed relatively low one, whereas manganese, nickel or cobalt were characterized by the lack of bactericidal action registered. Conclusion. Inhibitory action of heavy metal divalent ions on the lawns of S.pyogenes cultures probably includes bacteriostatic and bactericidal components which impact is determined by primary mechanisms of the ions toxicity.
{"title":"МЕХАНИЗМЫ БАКТЕРИЦИДНОГО ДЕЙСТВИЯ В РЕАЛИЗАЦИИ ОБЩИХ АНТИБАКТЕРИАЛЬНЫХ ЭФФЕКТОВ КАТИОНОВ МЕТАЛЛОВ В КУЛЬТУРЕ STREPTOCOCCUS PYOGENES","authors":"С. Б. Чекнев, Е. И. Вострова, С. В. Кисиль, М. А. Сарычева, А. В. Востров","doi":"10.36233/0372-9311-2018-2-3-9","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-3-9","url":null,"abstract":"Aim. The work was performed with the purpose to study an inhibitory action of millimolar concentrations of divalent metal ions, which differ by primary mechanisms of their toxicity, on the culture of S.pyogenes. Materials and methods. Suspensions of S.pyogenes bacteria which contained 10 8 CFU/ml were sown by the lawns into the standard Petri dishes coated with the supplemented Nutrient Agar. 30 min later the salt solutions of divalent metals were added by the 5 pl drops on the surfaces of the lawns with use of 36-channel stamp replicator. The salt solutions contained the metals tested at the concentrations ranged between 5x10 -3 M to 5x10 -1 M. Then the dishes with bacterial cultures were incubated for 24 hrs at 37°C followed by measuring diameter of the area of culture growth inhibition. The probes of material obtained from the centers of the stunting areas were passed into the centrifuge tubes with the supplemented Nutrient Broth, incubated for up to five days at 37°C and tested for the Broth clarity. Results. In presence of the metal concentrations ranged between 50 to 500 mM inhibitory action towards S.pyogenes bacteria was registered as relatively low due to the effects of copper or ferrous ions, as intermediate due to the presence of cobalt, nickel or manganese, and as high due to the effects of zinc ions. At the same time ferrous or copper ions demonstrated high bactericidal activity, zinc ions showed relatively low one, whereas manganese, nickel or cobalt were characterized by the lack of bactericidal action registered. Conclusion. Inhibitory action of heavy metal divalent ions on the lawns of S.pyogenes cultures probably includes bacteriostatic and bactericidal components which impact is determined by primary mechanisms of the ions toxicity.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78728169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-76-83
Л. П. Базанова, Елена Геннадьевна Токмакова, Гульнара Альфридовна Воронова, Сергей Балахонов
Aim. Influence of the plague agent plasmid content on biofilm formation in vivo and death rate of fleas-vectors with different vector activity in experiment were analyzed. Materials and methods. Three Yersinia pestis strains: virulent I-3230 (pYT, pYV, pYP) and I-2638 (pYT, pYV, pYP, pTP 33), and its selected avirulent isogenic clone I-3480 lacking two plasmids (pYV, pYP) were used. Three species of fleas were artificially infected: 477 individuals of Xenopsylla cheopis (a highly active vector), 441 - Citellophilus tesquorum (an active vector), 519 - Frontopsylla lucu-lenta (a low-active vector). The peculiarities of Y. pestis biofilm formation in fleas were estimated by a portion of individuals with bacterial «conglomerates» and «blocks» for a feeding. Death rate of the insects was defined by the percent of the dead fleas at each feeding. Results. All three flea species infected by Y. pestis strains carrying an additional plasmid pTP33 (I-2638 and I-3480) demonstrated the increase of the individual number with various biofilm forms in comparison with the three-plasmid strain I-3230. In X. cheopis it occurred due to the blocked insects, in C. tesquo-rum - mainly due to the fleas containing «conglomerates», in F. luculenta it was completely connected with ectoparasites with «conglomerates». A share of X. cheopis and C. tesquorum died at a feeding was higher in ectoparasites infected with I-3230 strain and F. luculenta - infected by I-2638. Conclusion. Y. pestis strains possessing an additional replicon pTP33 formed a biofilm in the infected insects more often and larger size than a classical three-plasmid variant. Influence of the strain plasmid content on death rate of the infected fleas depended on a vector species.
{"title":"ВЛИЯНИЕ ПЛАЗМИДНОГО СОСТАВА YERSINIA PESTIS НА ОБРАЗОВАНИЕ БИОПЛЕНКИ В ОРГАНИЗМЕ БЛОХ С РАЗНОЙ ВЕКТОРНОЙ АКТИВНОСТЬЮ","authors":"Л. П. Базанова, Елена Геннадьевна Токмакова, Гульнара Альфридовна Воронова, Сергей Балахонов","doi":"10.36233/0372-9311-2018-2-76-83","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-76-83","url":null,"abstract":"Aim. Influence of the plague agent plasmid content on biofilm formation in vivo and death rate of fleas-vectors with different vector activity in experiment were analyzed. Materials and methods. Three Yersinia pestis strains: virulent I-3230 (pYT, pYV, pYP) and I-2638 (pYT, pYV, pYP, pTP 33), and its selected avirulent isogenic clone I-3480 lacking two plasmids (pYV, pYP) were used. Three species of fleas were artificially infected: 477 individuals of Xenopsylla cheopis (a highly active vector), 441 - Citellophilus tesquorum (an active vector), 519 - Frontopsylla lucu-lenta (a low-active vector). The peculiarities of Y. pestis biofilm formation in fleas were estimated by a portion of individuals with bacterial «conglomerates» and «blocks» for a feeding. Death rate of the insects was defined by the percent of the dead fleas at each feeding. Results. All three flea species infected by Y. pestis strains carrying an additional plasmid pTP33 (I-2638 and I-3480) demonstrated the increase of the individual number with various biofilm forms in comparison with the three-plasmid strain I-3230. In X. cheopis it occurred due to the blocked insects, in C. tesquo-rum - mainly due to the fleas containing «conglomerates», in F. luculenta it was completely connected with ectoparasites with «conglomerates». A share of X. cheopis and C. tesquorum died at a feeding was higher in ectoparasites infected with I-3230 strain and F. luculenta - infected by I-2638. Conclusion. Y. pestis strains possessing an additional replicon pTP33 formed a biofilm in the infected insects more often and larger size than a classical three-plasmid variant. Influence of the strain plasmid content on death rate of the infected fleas depended on a vector species.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73280002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-83-87
О. В. Дуванова, Б. Н. Мишанькин, С. В. Титова, Л. А. Корнеева
Aim. To study the effect of N-acetyl-L-cysteine on biofilm of V. cholerae of different sero-groups isolated from various sources and with different epidemiological significance (the presence/ absence of the ctx AB genes and tcpA). Materials and methods. Bacterial eiiltiire of Vibrio cholerae El Tor O1 and O139 serogroups were grown as biolms. We have estimated the influence of the drug N-acetyl-L-cysteine at a concentration of 0.5 - 4 mg/ml on the formation, of the formed biofilm and in planktonic form. Results. Discovered antibacterial activity of N-acetyl-L-cysteine. Noted that it was influenced as in the formation, of the already formed biofilm and the planktonic form, the representatives of the Vibrio cholerae El Tor O1 and O139 serogroups in concentrations of 2 - 4 mg/ml, showing an antibacterial effect regardless of the presence/absence of genes ctx and tcpA AB. Conclusion. Identified the antibacterial action of the drug N-acetyl-L-cysteine against biofilm of V. cholerae indicates the desirability of considering the possibility of using drug therapy in cases variety of diseases caused by causative agents II - IV groups pathogenicity.
{"title":"ДЕЙСТВИЕ N-АЦЕТИЛ-L-ЦИСТЕИНА НА БИОПЛЕНКИ ХОЛЕРНОГО ВИБРИОНА","authors":"О. В. Дуванова, Б. Н. Мишанькин, С. В. Титова, Л. А. Корнеева","doi":"10.36233/0372-9311-2018-2-83-87","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-83-87","url":null,"abstract":"Aim. To study the effect of N-acetyl-L-cysteine on biofilm of V. cholerae of different sero-groups isolated from various sources and with different epidemiological significance (the presence/ absence of the ctx AB genes and tcpA). Materials and methods. Bacterial eiiltiire of Vibrio cholerae El Tor O1 and O139 serogroups were grown as biolms. We have estimated the influence of the drug N-acetyl-L-cysteine at a concentration of 0.5 - 4 mg/ml on the formation, of the formed biofilm and in planktonic form. Results. Discovered antibacterial activity of N-acetyl-L-cysteine. Noted that it was influenced as in the formation, of the already formed biofilm and the planktonic form, the representatives of the Vibrio cholerae El Tor O1 and O139 serogroups in concentrations of 2 - 4 mg/ml, showing an antibacterial effect regardless of the presence/absence of genes ctx and tcpA AB. Conclusion. Identified the antibacterial action of the drug N-acetyl-L-cysteine against biofilm of V. cholerae indicates the desirability of considering the possibility of using drug therapy in cases variety of diseases caused by causative agents II - IV groups pathogenicity.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76861632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-102-109
Y. Zakharova
Sagnelli E., Sagnelli C., Pisaturo M. 32. et al. Epidemiology of acute and chronic hepatitis B and delta over the last 5 decades in Italy. World J. Gastroenterol. 2014, 20 (24): 76357643. Sun X., Sui Q., Zhang C. et al. Targeting blockage of STAT3 in hepatocellular carcinoma cells 33. augments NK cell functions via reverse hepatocellular carcinoma-induced immune suppression. Mol. Cancer. Ther. 2013, 12 (12): 2885-2896. Taylor J.M. Virology of hepatitis D virus. Semin Liver Dis. 2012; 32 (3): 195-200. 34. Williams V., Brichler S., Radjef N. et al. Hepatitis delta virus proteins repress hepatitis B virus 35. enhancers and activate the alpha/beta interferon-inducible MxA gene. J. Gen. Virol. 2009, 90 (Pt. 11): 2759-2767. Williams V., Brichler S., Khan E. et al. Large hepatitis delta antigen activates STAT-3 and 36. NF-κB via oxidative stress. J. Viral. Hepat. 2012, 19 (10): 744-753.
{"title":"GUT BACTERIAL MICROBIOME OF HIV-INFECTED PEOPLE","authors":"Y. Zakharova","doi":"10.36233/0372-9311-2018-2-102-109","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-102-109","url":null,"abstract":"Sagnelli E., Sagnelli C., Pisaturo M. 32. et al. Epidemiology of acute and chronic hepatitis B and delta over the last 5 decades in Italy. World J. Gastroenterol. 2014, 20 (24): 76357643. Sun X., Sui Q., Zhang C. et al. Targeting blockage of STAT3 in hepatocellular carcinoma cells 33. augments NK cell functions via reverse hepatocellular carcinoma-induced immune suppression. Mol. Cancer. Ther. 2013, 12 (12): 2885-2896. Taylor J.M. Virology of hepatitis D virus. Semin Liver Dis. 2012; 32 (3): 195-200. 34. Williams V., Brichler S., Radjef N. et al. Hepatitis delta virus proteins repress hepatitis B virus 35. enhancers and activate the alpha/beta interferon-inducible MxA gene. J. Gen. Virol. 2009, 90 (Pt. 11): 2759-2767. Williams V., Brichler S., Khan E. et al. Large hepatitis delta antigen activates STAT-3 and 36. NF-κB via oxidative stress. J. Viral. Hepat. 2012, 19 (10): 744-753.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75685060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.36233/0372-9311-2018-2-109-119
A. Zemskov, V. Zemskov, V. Zemskova, R. Sepiashvili, Zoloedov Vi
Based on the data of the literature and their own studies, the features of infectious morbidity, immune disorders, the principles of their directed correction in patients at risk: pregnant women, puerperas, children born at physiological and pathological pregnancy, elderly persons are analyzed.
{"title":"INFECTION AND IMMUNITY IN PATIENTS WITH RISK GROUPS","authors":"A. Zemskov, V. Zemskov, V. Zemskova, R. Sepiashvili, Zoloedov Vi","doi":"10.36233/0372-9311-2018-2-109-119","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-109-119","url":null,"abstract":"Based on the data of the literature and their own studies, the features of infectious morbidity, immune disorders, the principles of their directed correction in patients at risk: pregnant women, puerperas, children born at physiological and pathological pregnancy, elderly persons are analyzed.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84907250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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