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REGULATION OF MICROORGANISMS SENSITIVITY TO ANTAGONISTIC ACTIVE REPRESENTATIVES OF MUTUALISTICALLY MICROFLORA BY THE ANTIMICROBIAL PEPTIDES 微生物对共生菌群拮抗活性代表物敏感性的抗菌肽调控
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-21-25
M. V. Sycheva, Yuliya Peshkova, O. L. Kartashova, A. Andreeva
Aim. Study of the effect of antimicrobial peptides (AMP) on the sensitivity of the conditional pathogenic of bacteria to the action of antagonistic active of representatives mutualistically microflora. Materials and methods. The study used peptides from platelets of chicken obtained by the method of reversed-phase high-performance liquid chromatography in stepwise and linear gradients of increasing concentrations of organic solvent. The effect of AMP on the sensitivity of microorganisms to antagonistic active substances of the dominant bacteria of the genus Lactobacillus, and Enterococcus were investigated in a joint incubation. Antagonistic activity of bacteria studied the gup method. Results. Shown an increased expression of antagonistic activity of indigenous microflora in relation to associative microflora after concubinage with a peptide fractions from platelets of chicken. Conclusion. The obtained results allow to assume the existence of new mechanisms of formation of the colonization resistance of the biotope.
的目标。抗菌肽(AMP)对条件致病菌对代表性共生菌群拮抗活性作用敏感性的影响研究。材料和方法。本研究采用反相高效液相色谱法从鸡血小板中提取多肽,有机溶剂浓度呈逐步线性梯度增加。在联合培养实验中,研究了AMP对乳酸菌属和肠球菌属优势菌拮抗活性物质敏感性的影响。用分组法研究细菌的拮抗活性。结果。结果表明,与鸡血小板的肽组分相比较,与结合菌群相比,本地菌群的拮抗活性增加。结论。所获得的结果允许假设存在新的机制形成的定植抵抗的生物群落。
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引用次数: 2
FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE 克罗恩病患者分离的大肠杆菌临床菌株的特征
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-42-49
Roman Gorodnichev, D. Rakitina, A. Manolov, Yu. P. Baykova, P. Scherbakov, G. B. Smirnov, E. Ilina
Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall.
的目标。从克罗恩病患者中分离出与克罗恩病相关的大肠杆菌。材料和方法。临床染色28例。对临床分离株和对照样品进行了可能属于某一系统发育类群、粘附和侵入单层CaCo2上皮细胞模型的能力、形成生物膜的能力和迁移能力的测试。结果。我们已经证明,从不同的生物材料中分离出来的大肠杆菌属于不同的系统发育群,它们形成生物膜的能力也不同。黏附侵袭性大肠杆菌主要见于克罗恩病患者肠黏膜活检材料中。结论。有理由认为黏附侵袭性大肠杆菌是在肠壁和肠腔生态位的交界处形成的。
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引用次数: 0
ESTIMATE OF VIRUS ZIKA SPREAD RISK IN THE REPUBLIC OF ABKHAZIA ASSOCIATING THE LOCAL POPULATION OF MOSQUITOES AEDES AEGYPTI AND AEDES ALBOPICTUS 阿布哈兹共和国与埃及伊蚊和白纹伊蚊当地种群有关的寨卡病毒传播风险估计
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-10-15
O. Maletskaya, V. Dubyansky, A. Belyaeva, L. Shaposhnikova, Aga Ds, N. Ermolova, N. V. Tsapko, T. Taran, E. Kotenev, O. Belova, A. Kulichenko
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引用次数: 0
ПРОТОЧНО-ЦИТОФЛУОРИМЕТРИЧЕСКИЙ АНАЛИЗ ЦИТОТОКСИЧНОСТИ ШТАММОВ YERSINIA PESTIS
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-3-9
E. V. Sazanova, T. P. Shmelkova, A. L. Kravtsov, T. A. Malyukova, Yu. A. Popov
Aim. Study cytotoxic effect of Yersinia pestis strains with various degree of virulence on leukocytes of whole defibrinated human blood. Materials and methods. 15 strains of Y. pestis were used in the study. DNA histograms were registered using flow cytofluorimeter. Results. Possibility of using cytotoxicity to differentiate Y. pestis into virulent and avirulent w f as experimentally proven. The data obtained was comparable with LD 5 o for mice and did not contradict molecular-genetic characteristics of the strains. Conclusion. The approach proposed allows to obtain reliable results and is perspective for evaluation of virulence for Y. pestis strains.
的目标。研究不同毒力鼠疫耶尔森菌菌株对人全血白细胞的细胞毒作用。材料和方法。采用15株鼠疫菌进行研究。用流式细胞荧光仪记录DNA直方图。结果。利用细胞毒性将鼠疫杆菌区分为毒力型和无毒型的可能性已得到实验证实。获得的数据与小鼠的ld50相当,并且与菌株的分子遗传特征不矛盾。结论。提出的方法可以获得可靠的结果,并为评估鼠疫杆菌菌株的毒力提供了前景。
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引用次数: 1
ОБЩАЯ ХАРАКТЕРИСТИКА И ПРОБЛЕМНЫЕ ВОПРОСЫ ПОЛИЭТИОЛОГИЧНЫХ ИНФЕКЦИЙ, ВЫЗЫВАЕМЫХ УСЛОВНО ПАТОГЕННЫМИ МИКРООРГАНИЗМАМИ 由病原体引起的多病原体感染的一般特征和问题
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-114-126
Вл В. Шкарин, Ольга Васильевна Ковалишена, Н. В. Саперкин, О. Н. Шпрыкова
Infections caused by opportunistic microorganisms occupy a special position. In microbial associations of opportunistic microorganisms is easier to implement its low pathogenic potential, activate properties of each other. Group polyetiologic infections caused by conditionally pathogenic microorganisms are quite diverse in terms of a nosological forms and groups. These include the so-called septic infection, which are also very diverse and clinical, epidemiological and etiological characteristics. Scientific evidence shows the urgency of the problem mixed infections, especially in surgery and indicate a lack of systematic data about the whole spectrum and the composition of microbial associations in different infections, quantification, insufficient study of the properties compared to monoagents. The problem mixed infections practically not been studied with epidemiologic standpoint, only a few studies devoted to the study of nosocomial infection of microbial associations are not developed microbiological monitoring. In this connection, you need comprehensive research mixed infections compared with monoagents to identify the characteristics and development of epidemiologic surveillance and control.
机会微生物引起的感染占有特殊地位。在微生物协会中,机会微生物更容易实现其低致病性、相互激活的特性。由条件性病原微生物引起的群体性多病原感染在病原学形式和类群上是相当多样化的。这些包括所谓的脓毒性感染,它也非常多样化和临床,流行病学和病原学特征。科学证据表明,混合感染问题的紧迫性,特别是在外科手术中,并表明缺乏关于不同感染的全谱和微生物关联组成的系统数据,量化,与单一药物相比,对其特性的研究不足。混合感染问题目前还没有从流行病学的角度进行研究,只有少数研究致力于研究微生物关联的医院感染,而没有开展微生物监测。在这方面,需要综合研究混合感染与单药感染的比较,以确定流行病学监测和控制的特点和发展。
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引用次数: 0
EPIDEMIC ANALYSIS ON CHOLERA IN AFRICA AND PROBLEMS OF PROFILAXIS 非洲霍乱流行病学分析及相关问题
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-105-113
I. G. Karnaukhov, N. B. Cheldyshova, A. K. Grazhdanov, A. A. Krizky, S. P. Zadnova, O. V. Kedrova, A. V. Ivanova, V. Toporkov, N. Smirnova, A. .. Boiro, S. Boumbali, M. Boiro, V. Kutyrev
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引用次数: 0
MOLECULAR AND GENETIC FEATURES OF THE METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) - CAUSATIVE AGENTS OF PURULENT DISEASES AT CANCER PATIENTS 耐甲氧西林金黄色葡萄球菌(mrsa) -癌症患者化脓性疾病病原体的分子和遗传特征
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-15-20
O. Khokhlova, O. Peryanova, O. Bobrova, V. K. Sergeeva, A. A. Modestov, O. G. Eremeeva, N. K. Potkina, D. N. Kapshuk, A. V. Alabusheva, Tatsuo Yamamoto
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引用次数: 2
EPIDEMIOLOGICAL STUDY OF MAIN CLONES DISTRIBUTION GENOTYPE BEIJING OF MYCOBACTERIUM TUBERCULOSIS WITHIN IRKUTSK REGION 伊尔库茨克地区结核分枝杆菌北京基因型主要克隆分布的流行病学研究
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-88-94
Н. Жданова, ' О.Б.Огарков, В. Синьков, ПЛ. Хромова, Е. А. Орлова, М. Е. Кощеев, Е. Д. Савилов, N. Zhdanova, O. Ogarkov, V. Sinkov, P. Khromova, E. Orlova, M. Koscheev, E. Savilov
To the pathogen in the Irkutsk region by comparison of genotypes of M. tuberculosis from patients of different age groups. Materials and methods. 588 epidemiologically unrelated strains of M. tuber­ culosis isolates from 567 ТВ patients were characterized using 24-locus MIRU-VNTR typing. 160 strains belonged to patients of different age groups. 59 strains were isolated from the «young» people with ТВ who were bom after 1990 and 101 isolates from people who were bom before 1955. Results. Two-thirds of the samples (427/567) were genotype Beijing with the dominance of two subtypes belonging to the modem epidemic clonal complexes CC1 and CC2. The high level of clustering profiles of СС 1 and CC2 genotype Beijing in «older» and «young» patients in the Irkutsk region indicates the presence and active transmission of epidemic CC1 and CC2 subtypes over the past fifty years.
对伊尔库茨克地区不同年龄组患者结核分枝杆菌的基因型进行比较。材料和方法。采用24位点MIRU-VNTR分型对567例ТВ患者分离的588株无流行病学相关性的结核分枝杆菌进行了鉴定。160株属于不同年龄组的患者。从1990年以后出生的患有ТВ的“年轻人”中分离出59株菌株,从1955年以前出生的人中分离出101株菌株。结果。其中2 / 3(427/567)为北京型,以现代流行克隆复合物CC1和CC2亚型为主。伊尔库茨克地区“老年”和“年轻”患者中СС 1和CC2基因型北京的高水平聚类特征表明,过去50年来流行CC1和CC2亚型的存在和活跃传播。
{"title":"EPIDEMIOLOGICAL STUDY OF MAIN CLONES DISTRIBUTION GENOTYPE BEIJING OF MYCOBACTERIUM TUBERCULOSIS WITHIN IRKUTSK REGION","authors":"Н. Жданова, ' О.Б.Огарков, В. Синьков, ПЛ. Хромова, Е. А. Орлова, М. Е. Кощеев, Е. Д. Савилов, N. Zhdanova, O. Ogarkov, V. Sinkov, P. Khromova, E. Orlova, M. Koscheev, E. Savilov","doi":"10.36233/0372-9311-2017-6-88-94","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-88-94","url":null,"abstract":"To the pathogen in the Irkutsk region by comparison of genotypes of M. tuberculosis from patients of different age groups. Materials and methods. 588 epidemiologically unrelated strains of M. tuber­ culosis isolates from 567 ТВ patients were characterized using 24-locus MIRU-VNTR typing. 160 strains belonged to patients of different age groups. 59 strains were isolated from the «young» people with ТВ who were bom after 1990 and 101 isolates from people who were bom before 1955. Results. Two-thirds of the samples (427/567) were genotype Beijing with the dominance of two subtypes belonging to the modem epidemic clonal complexes CC1 and CC2. The high level of clustering profiles of СС 1 and CC2 genotype Beijing in «older» and «young» patients in the Irkutsk region indicates the presence and active transmission of epidemic CC1 and CC2 subtypes over the past fifty years.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"26 1","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83918871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
ВЛИЯНИЕ МЕТОДОВ ИММОБИЛИЗАЦИИ ЭРИТРОПОЭТИНА НА ЧУВСТВИТЕЛЬНОСТЬ ВЫЯВЛЕНИЯ СПЕЦИФИЧЕСКИХ IgG К EПB В СЫВОРОТКАХ КРОВИ ЭКСПЕРИМЕНТАЛЬНЫЙ ЖИВОТНЫХ 血液血清中的IgG对epb的敏感性影响是实验性的。
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-49-55
A. Kudryashova, O. Borisova, N. Mikhailova, D. V. Lonshakov, A. V. Katlinsky
Aim. The study of the effect of the erythropoietin coating procedure on sensitivity using the same secondary detection methods to quantify anti-EPO IgG positive animal sera. Materials and methods. Sera from experimental animals - rabbits and guinea pigs - after rhEPO injection were used. The methods includes directly coated ELISA and two types of immunochemical immobilization: capturing biotinylated rhEPO on streptavidin coated microtiier plates and capturing rhEPO via a specific antibody Results. Immunochemical rhEPO immobilization results in a sensitivity from 2 to 10 of magnitude higher than direct coating of rhEPO. Conclusion. Our findings show that the method of rhEPO immobilization to microtiter plates is a critical determinant for the sensitivity of ELISA used for measuring anti-EPO antibodies. Assays in which rhEPO was captured via a specific mAb, or in which biotinylated rhEPO was captured via streptavidin, are preferred to detect serum antibodies to native structural state.
的目标。采用相同的二次检测方法定量抗epo IgG阳性动物血清,研究促红细胞生成素包衣程序对敏感性的影响。材料和方法。使用rhEPO注射后的实验动物(兔和豚鼠)血清。方法包括直接包被ELISA和两种类型的免疫化学固定:在链霉亲和素包被的微孔板上捕获生物素化的rhEPO和通过特异性抗体捕获rhEPO。免疫化学rhEPO固定的结果从2到10量级的灵敏度高于rhEPO直接包衣。结论。我们的研究结果表明,rhEPO固定在微滴板上的方法是用于测定抗epo抗体的ELISA灵敏度的关键决定因素。通过特异性单抗捕获rhEPO或通过链霉亲和素捕获生物素化rhEPO的检测更适合检测天然结构状态的血清抗体。
{"title":"ВЛИЯНИЕ МЕТОДОВ ИММОБИЛИЗАЦИИ ЭРИТРОПОЭТИНА НА ЧУВСТВИТЕЛЬНОСТЬ ВЫЯВЛЕНИЯ СПЕЦИФИЧЕСКИХ IgG К EПB В СЫВОРОТКАХ КРОВИ ЭКСПЕРИМЕНТАЛЬНЫЙ ЖИВОТНЫХ","authors":"A. Kudryashova, O. Borisova, N. Mikhailova, D. V. Lonshakov, A. V. Katlinsky","doi":"10.36233/0372-9311-2017-6-49-55","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-49-55","url":null,"abstract":"Aim. The study of the effect of the erythropoietin coating procedure on sensitivity using the same secondary detection methods to quantify anti-EPO IgG positive animal sera. Materials and methods. Sera from experimental animals - rabbits and guinea pigs - after rhEPO injection were used. The methods includes directly coated ELISA and two types of immunochemical immobilization: capturing biotinylated rhEPO on streptavidin coated microtiier plates and capturing rhEPO via a specific antibody Results. Immunochemical rhEPO immobilization results in a sensitivity from 2 to 10 of magnitude higher than direct coating of rhEPO. Conclusion. Our findings show that the method of rhEPO immobilization to microtiter plates is a critical determinant for the sensitivity of ELISA used for measuring anti-EPO antibodies. Assays in which rhEPO was captured via a specific mAb, or in which biotinylated rhEPO was captured via streptavidin, are preferred to detect serum antibodies to native structural state.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"16 5 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87994937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT ORF3 PROTEIN BY CODON OPTIMIZATION METHOD 戊型肝炎病毒基因1型重组orf3蛋白的密码子优化设计
Q3 Medicine Pub Date : 2017-12-28 DOI: 10.36233/0372-9311-2017-6-63-72
G. Alatortseva, Sidorov Av, L. Nesterenko, L. Luhverchik, M. V. Zhukina, I. I. Amiantova, A. Milovanova, D. Vorobev, Yu. I. Ammur, M. Mikhailov, K. K. Kyuregyan, V. Kichatova, I. Potemkin, O. Isaeva, E. Malinnikova, A. Karlsen, V. Blinov, Z. Nurmatov, A. Z. Nurmatov, O. Kasymov, S. Zhavoronok, Zverev Vv
sensitivity to antagonistic active representatives of mutualistically microflora by the antimicrobial peptides
抗菌肽对共生菌群拮抗活性代表的敏感性
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引用次数: 2
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Zhurnal mikrobiologii, epidemiologii, i immunobiologii
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