Pub Date : 2020-03-06DOI: 10.36233/0372-9311-2020-97-1-72-82
A. Y. Zaitsev, E. A. Kurbatova, N. B. Egorova, E. V. Sukhova, Nikolay E. Nifantiev
The introduction of pneumococcal vaccines into national immunization programmes around the world has reduced the incidence of pneumococcal vaccine serotypes, but had no influence on the incidence of Streptococcus pneumoniae serotype 3 included in their composition. The results of evaluation of epidemiological efficacy and immunogenicity of capsular polysaccharide of S. pneumoniae serotype 3 capsular polysaccharide (CP) in conjugated and polysaccharide pneumococcal vaccines are contradictory. Some studies have shown the effectiveness of vaccination, other studies indicate insufficient immunogenicity and prophylactic efficacy of S. pneumoniae serotype 3 CP. The authors’ analysis of the results of clinical studies showed that the prophylactic efficacy of S. pneumoniae serotype 3 CP depends on the type of vaccine, nosological form of the disease, age, immunization schedule. According to the literature data, the most informative parameter of the protective activity of S. pneumoniae CP in pneumococcal vaccines, including serotype 3, is opsonophagocytosis. The experimental data of the low immunogenicity of serotype 3 CP, presumably associated with an unusual way of synthesis of its CP, are considered. To increase the im muno genicity of S. pneumoniae serotype 3 CP, the use of synthetic oligosaccharides of a strictly defined chemical structure corresponding to the protective fragments of serotype 3 CP and conjugated with a carrier protein for induction of T-dependent immune response and immunological memory is promising.
{"title":"Иммунологические и эпидемиологические аспекты иммуногенности капсульного полисахарида Streptococcus pneumoniae серотипа 3 в составе пневмококковых вакцин","authors":"A. Y. Zaitsev, E. A. Kurbatova, N. B. Egorova, E. V. Sukhova, Nikolay E. Nifantiev","doi":"10.36233/0372-9311-2020-97-1-72-82","DOIUrl":"https://doi.org/10.36233/0372-9311-2020-97-1-72-82","url":null,"abstract":"The introduction of pneumococcal vaccines into national immunization programmes around the world has reduced the incidence of pneumococcal vaccine serotypes, but had no influence on the incidence of Streptococcus pneumoniae serotype 3 included in their composition. The results of evaluation of epidemiological efficacy and immunogenicity of capsular polysaccharide of S. pneumoniae serotype 3 capsular polysaccharide (CP) in conjugated and polysaccharide pneumococcal vaccines are contradictory. Some studies have shown the effectiveness of vaccination, other studies indicate insufficient immunogenicity and prophylactic efficacy of S. pneumoniae serotype 3 CP. The authors’ analysis of the results of clinical studies showed that the prophylactic efficacy of S. pneumoniae serotype 3 CP depends on the type of vaccine, nosological form of the disease, age, immunization schedule. According to the literature data, the most informative parameter of the protective activity of S. pneumoniae CP in pneumococcal vaccines, including serotype 3, is opsonophagocytosis. The experimental data of the low immunogenicity of serotype 3 CP, presumably associated with an unusual way of synthesis of its CP, are considered. To increase the im muno genicity of S. pneumoniae serotype 3 CP, the use of synthetic oligosaccharides of a strictly defined chemical structure corresponding to the protective fragments of serotype 3 CP and conjugated with a carrier protein for induction of T-dependent immune response and immunological memory is promising.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85558450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-06DOI: 10.36233/0372-9311-2020-97-1-47-54
N. A. Selyanskaya, E. A. Bereznyak, A. V. Trishina, I. R. Simonova, L. A. Egiazaryan, A. S. Vodopyanov
Aim . The aim was to develop an integrated online and updated geographic information system (GIS) for the systematiza tion and analysis of information on V. cholerae El Tor antibiotic resistance. Material and methods . The method of serial dilutions in a dense nutrient medium (MUK 4.2.2495-09) was used to determine the sensitivity/resistance to 22 antibacterial preparations of V. cholerae O1 El Tor strains isolated from people and from aquatic environmental objects in Russia in 2005–2016. The development of the Internet version of the GIS was carried out using programming languages HTML, JavaScript and PHP and cartographic data obtained from the corporation Rostelecom (Russia) and the community OpenStreetMap. Results . A comparative analysis of the antibiotic resistance of the strains by regions of the Russian Federation and by isolation time showed an increase of strains resistant to streptomycin, ampicillin, rifampicin and furazolidone, the appearance of cultures resistant to nalidixic acid and ceftriaxone, reduced resistance to trimethoprim/sulfamethoxazole in 2012–2016 compared to 2005–2009. In the Stavropol Krai, the isolated V. cholerae O1 El Tor were characterized by resistance to furazolidone (33.3%) and trimethoprim/sulfamethoxazole (100%); in the Primorsky Krai — to ampicillin, streptomycin, rifampicin (7%), furazolidone (43%), trimethoprim/sulfamethoxazole (100%); in the Irkutsk region and Kalmykia, to ampicillin (8.3% and 11%), furazolidone and trimethoprim/sulfamethoxazole (11% and 89%). Conclusion . The GIS developed allows to collect and analyze information on the antibiotic resistance of V. cholerae O1 El Tor, and to select the strains for given properties.
的目标。目的是开发一个综合的在线和更新的地理信息系统(GIS)，以便系统化和分析关于霍乱弧菌El - Tor抗生素耐药性的信息。材料和方法。采用密集营养培养基(MUK 4.2.2495-09)连续稀释法测定2005-2016年从俄罗斯人群和水生环境物分离的霍乱弧菌O1 El - Tor菌株对22种抗菌制剂的敏感性/耐药性。互联网版GIS的开发使用了HTML、JavaScript和PHP编程语言，以及从Rostelecom(俄罗斯)公司和OpenStreetMap社区获得的地图数据。结果。俄罗斯联邦各地区和分离时间菌株耐药性对比分析显示，与2005-2009年相比，2012-2016年对链霉素、氨苄西林、利福平和呋喃唑酮耐药菌株增加，对萘啶酸和头孢曲松耐药菌株出现，对甲氧苄啶/磺胺甲恶唑耐药降低。斯塔夫罗波尔地区分离的霍乱弧菌o1el - Tor对呋喃唑酮(33.3%)和甲氧苄啶/磺胺甲恶唑(100%)耐药;在滨海边疆区-氨苄西林、链霉素、利福平(7%)、呋喃唑酮(43%)、甲氧苄啶/磺胺甲恶唑(100%);在伊尔库茨克地区和卡尔梅克，分别为氨苄西林(8.3%和11%)、呋喃唑酮和甲氧苄啶/磺胺甲恶唑(11%和89%)。结论。开发的地理信息系统允许收集和分析霍乱弧菌O1 El - Tor的抗生素耐药性信息，并根据给定的特性选择菌株。
{"title":"Пространственная визуализация данных по антибиотикорезистентности штаммов холерных вибрионов, изолированных на территории России","authors":"N. A. Selyanskaya, E. A. Bereznyak, A. V. Trishina, I. R. Simonova, L. A. Egiazaryan, A. S. Vodopyanov","doi":"10.36233/0372-9311-2020-97-1-47-54","DOIUrl":"https://doi.org/10.36233/0372-9311-2020-97-1-47-54","url":null,"abstract":"Aim . The aim was to develop an integrated online and updated geographic information system (GIS) for the systematiza tion and analysis of information on V. cholerae El Tor antibiotic resistance. Material and methods . The method of serial dilutions in a dense nutrient medium (MUK 4.2.2495-09) was used to determine the sensitivity/resistance to 22 antibacterial preparations of V. cholerae O1 El Tor strains isolated from people and from aquatic environmental objects in Russia in 2005–2016. The development of the Internet version of the GIS was carried out using programming languages HTML, JavaScript and PHP and cartographic data obtained from the corporation Rostelecom (Russia) and the community OpenStreetMap. Results . A comparative analysis of the antibiotic resistance of the strains by regions of the Russian Federation and by isolation time showed an increase of strains resistant to streptomycin, ampicillin, rifampicin and furazolidone, the appearance of cultures resistant to nalidixic acid and ceftriaxone, reduced resistance to trimethoprim/sulfamethoxazole in 2012–2016 compared to 2005–2009. In the Stavropol Krai, the isolated V. cholerae O1 El Tor were characterized by resistance to furazolidone (33.3%) and trimethoprim/sulfamethoxazole (100%); in the Primorsky Krai — to ampicillin, streptomycin, rifampicin (7%), furazolidone (43%), trimethoprim/sulfamethoxazole (100%); in the Irkutsk region and Kalmykia, to ampicillin (8.3% and 11%), furazolidone and trimethoprim/sulfamethoxazole (11% and 89%). Conclusion . The GIS developed allows to collect and analyze information on the antibiotic resistance of V. cholerae O1 El Tor, and to select the strains for given properties.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91130699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-06DOI: 10.36233/0372-9311-2020-97-1-91-101
A. Belov, A. Panin
The article represents the history of studies of human diseases caused by faсultative parasites of humans, animals and plants, attributed by microbiologist V.I. Terskych in 1958 to the third class of infections named sapronoses. The concept of sapronous pathogens ecology is one of priorities of Russian medicine. Since that time, facts and generalizations have been accumulated in the world and national science, allowing to revise the initial concept of sapronous infections of biota — from prokaryotes, plants and invertebrates to warm-blooded animals and humans. To decide the controversial issues of the sapronoses theory, epidemiological terminology and systematics of infectious pathology, it is necessary to integrate the knowledge and systemic participation of specialists in medicine and biology in studying this problem. The results of this cooperation will make it possible to clarify and form a unified theoretical concept of the population pathology of the biota based on the ecological approach and general biological regularities of life existence in the world.
{"title":"ТЕОРИЯ САПРОНОЗНЫХ ИНФЕКЦИЙ: ИСТОРИЯ РАЗВИТИЯ И ПУТИ СОВЕРШЕНСТВОВАНИЯ В СИСТЕМЕ МЕДИКО-БИОЛОГИЧЕСКИХ НАУК","authors":"A. Belov, A. Panin","doi":"10.36233/0372-9311-2020-97-1-91-101","DOIUrl":"https://doi.org/10.36233/0372-9311-2020-97-1-91-101","url":null,"abstract":"The article represents the history of studies of human diseases caused by faсultative parasites of humans, animals and plants, attributed by microbiologist V.I. Terskych in 1958 to the third class of infections named sapronoses. The concept of sapronous pathogens ecology is one of priorities of Russian medicine. Since that time, facts and generalizations have been accumulated in the world and national science, allowing to revise the initial concept of sapronous infections of biota — from prokaryotes, plants and invertebrates to warm-blooded animals and humans. To decide the controversial issues of the sapronoses theory, epidemiological terminology and systematics of infectious pathology, it is necessary to integrate the knowledge and systemic participation of specialists in medicine and biology in studying this problem. The results of this cooperation will make it possible to clarify and form a unified theoretical concept of the population pathology of the biota based on the ecological approach and general biological regularities of life existence in the world.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84417921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-05DOI: 10.36233/0372-9311-2020-97-1-15-18
Игорь Дмитриевич Колесин, Екатерина Михайловна Житкова
The aim of the study is to find a quantitative relationship between antigenic distances (AD) and vaccination effectiveness (VE) and investigate the response of VE to changes in AD. Material and methods . Through the epidemiological data of three influenza seasons in Canada, interpreted within the framework of the antigenic distance hypothesis (ADH), the introduction of the correction factor into the model for estimating VE was substantiated considering the antigenic relationship between the previous season vaccine (V1), the current season vaccine (V2) and the circulating epidemic strain (e). Results . A quantitative relationship between VE and AD was found, reproducing the results of epidemiological observation of two groups of people: vaccinated in the previous and current seasons (V1+V2) and vaccinated only in the current season (0+V2). The difference in the response of VE to different indicators of AD was found and allowing to use only one significant indicator. Conclusion . Тhe model proposed relates the microbiological indicators AD with epidemiological characteristic of VE. The model extends the analysis, allowing to use it as an assessment tool for the expected changes in vaccine effectiveness in various settings of the ADH hypothesis experiment.
{"title":"Модель профилактической эффективности противогриппозной вакцины с поправкой на гипотезу антигенного расстояния","authors":"Игорь Дмитриевич Колесин, Екатерина Михайловна Житкова","doi":"10.36233/0372-9311-2020-97-1-15-18","DOIUrl":"https://doi.org/10.36233/0372-9311-2020-97-1-15-18","url":null,"abstract":"The aim of the study is to find a quantitative relationship between antigenic distances (AD) and vaccination effectiveness (VE) and investigate the response of VE to changes in AD. Material and methods . Through the epidemiological data of three influenza seasons in Canada, interpreted within the framework of the antigenic distance hypothesis (ADH), the introduction of the correction factor into the model for estimating VE was substantiated considering the antigenic relationship between the previous season vaccine (V1), the current season vaccine (V2) and the circulating epidemic strain (e). Results . A quantitative relationship between VE and AD was found, reproducing the results of epidemiological observation of two groups of people: vaccinated in the previous and current seasons (V1+V2) and vaccinated only in the current season (0+V2). The difference in the response of VE to different indicators of AD was found and allowing to use only one significant indicator. Conclusion . Тhe model proposed relates the microbiological indicators AD with epidemiological characteristic of VE. The model extends the analysis, allowing to use it as an assessment tool for the expected changes in vaccine effectiveness in various settings of the ADH hypothesis experiment.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72957612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. A. Markin, V. B. Pantiukhov, V. I. Markov, V. Bondarev
Features of the Argentine hemorrhagic fever are described in the review. Epidemiology, etiology, clinical presentation and pathogenesis of the disease are examined. Special consideration is given to the features of the pathological agent of Argentine hemorrhagic fever--the Junin virus. Features of the disease diagnostics are indicated--virological and serological studies, immunochemical and molecular-biological methods of identification of the pathological agent and antibodies against it. Approaches to etiotropic therapy of this disease and vaccination are examined. Based on the foreign experience perspective guidance for the creation of the system of protection of the population of the Russian Federation against Argentine hemorrhagic fever are presented.
{"title":"[Argentine hemorrhagic fever].","authors":"V. A. Markin, V. B. Pantiukhov, V. I. Markov, V. Bondarev","doi":"10.32388/f9dtvh","DOIUrl":"https://doi.org/10.32388/f9dtvh","url":null,"abstract":"Features of the Argentine hemorrhagic fever are described in the review. Epidemiology, etiology, clinical presentation and pathogenesis of the disease are examined. Special consideration is given to the features of the pathological agent of Argentine hemorrhagic fever--the Junin virus. Features of the disease diagnostics are indicated--virological and serological studies, immunochemical and molecular-biological methods of identification of the pathological agent and antibodies against it. Approaches to etiotropic therapy of this disease and vaccination are examined. Based on the foreign experience perspective guidance for the creation of the system of protection of the population of the Russian Federation against Argentine hemorrhagic fever are presented.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47365707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-19DOI: 10.36233/0372-9311-2019-5-109-118
Е. С. Шипко, О. В. Дуванова
The review presents the basic information available in the literature on changes in the composition of fatty acids in various microorganisms in response to various environmental factors (stressors). The issues affecting the importance of fatty acids as biomarkers of pathogenetic and adaptive-persistent potential of bacteria are discussed. The prospects of studying the spectrum of fatty acids in the field of biochemistry, in particular, lipidomics of infectious diseases are noted.
{"title":"Изменение спектра жирных кислот как один из механизмов адаптации/персистенции микроорганизмов","authors":"Е. С. Шипко, О. В. Дуванова","doi":"10.36233/0372-9311-2019-5-109-118","DOIUrl":"https://doi.org/10.36233/0372-9311-2019-5-109-118","url":null,"abstract":"The review presents the basic information available in the literature on changes in the composition of fatty acids in various microorganisms in response to various environmental factors (stressors). The issues affecting the importance of fatty acids as biomarkers of pathogenetic and adaptive-persistent potential of bacteria are discussed. The prospects of studying the spectrum of fatty acids in the field of biochemistry, in particular, lipidomics of infectious diseases are noted.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78564586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.1891/9780826195197.0060
E. N. Torganova, S. A. Abdullaeva, M. Boichenko
{"title":"[Bacterial vaginosis].","authors":"E. N. Torganova, S. A. Abdullaeva, M. Boichenko","doi":"10.1891/9780826195197.0060","DOIUrl":"https://doi.org/10.1891/9780826195197.0060","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44557742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-28DOI: 10.36233/0372-9311-2018-6-31-37
N. Mihailova, A. A. Kaloshin, E. Zimina, A. Soldatenkova, A. V. Poddubikov
Aim. Evaluation of the efficacy and safety of the three experimental lots of Recombinant Pseudomonas Vaccine. Materials and methods. The preparation contained the recombinant proteins OprF and toxoid that were purified by chromatography in nickel-sepharose. Aluminum hydroxide was used as an adjuvant. The authenticity of the vaccine components was confirmed by electrophoresis and immunoblotting. The concentration of endotoxin in the vaccine was determined by LAL test. The abnormal toxicity was evaluated in mice and cavy. The anaphylactic activity was evaluated in cavy. The delayed-type hypersensitivity reaction was evaluated in mice. Evaluation of the immunogenicity was carried out in an experiment with on double immunization of mice with following intraperitoneally infection by a live virulent culture of P. aeruginosa (PA-103 strain). Results. The authenticity of the vaccine, sterility, non-pyrogenicity and non-toxicity were confirmed after the studying of the quality indicators of the three lots of Recombinant Pseudomonas Vaccine. In animal experiments, the vaccine did not possess allergic properties and it was shown that it protected mice against Pseudomonas infection with Index of efficiency 3.0 and more. Conclusion. The efficacy, the safety, and the standardization of three experimental lots of the recombinant vaccine, which is intended to prevent infections caused by P. aeruginosa, have been shown.
{"title":"ДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ РЕКОМБИНАНТНОЙ ВАКЦИНЫ СИНЕГНОЙНОЙ","authors":"N. Mihailova, A. A. Kaloshin, E. Zimina, A. Soldatenkova, A. V. Poddubikov","doi":"10.36233/0372-9311-2018-6-31-37","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-31-37","url":null,"abstract":"Aim. Evaluation of the efficacy and safety of the three experimental lots of Recombinant Pseudomonas Vaccine. Materials and methods. The preparation contained the recombinant proteins OprF and toxoid that were purified by chromatography in nickel-sepharose. Aluminum hydroxide was used as an adjuvant. The authenticity of the vaccine components was confirmed by electrophoresis and immunoblotting. The concentration of endotoxin in the vaccine was determined by LAL test. The abnormal toxicity was evaluated in mice and cavy. The anaphylactic activity was evaluated in cavy. The delayed-type hypersensitivity reaction was evaluated in mice. Evaluation of the immunogenicity was carried out in an experiment with on double immunization of mice with following intraperitoneally infection by a live virulent culture of P. aeruginosa (PA-103 strain). Results. The authenticity of the vaccine, sterility, non-pyrogenicity and non-toxicity were confirmed after the studying of the quality indicators of the three lots of Recombinant Pseudomonas Vaccine. In animal experiments, the vaccine did not possess allergic properties and it was shown that it protected mice against Pseudomonas infection with Index of efficiency 3.0 and more. Conclusion. The efficacy, the safety, and the standardization of three experimental lots of the recombinant vaccine, which is intended to prevent infections caused by P. aeruginosa, have been shown.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86448021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-28DOI: 10.36233/0372-9311-2018-6-66-72
Д. В. Ульшина, Е. И. Еременко, Дмитрий Александрович Ковалев, Алла Рязанова, Ирина Валерьевна Кузнецова, Л. Ю. Аксенова, О. В. Семенова, О. В. Бобрышева, Ю. В. Сирица, Александр Куличенко
Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.
{"title":"ВЫЯВЛЕНИЕ ОСОБЕННОСТЕЙ МАСС-СПЕКТРОВ БЕЛКОВЫХ ЭКСТРАКТОВ СПОРОВОЙ И ВЕГЕТАТИВНОЙ ФОРМ ВОЗБУДИТЕЛЯ СИБИРСКОЙ ЯЗВЫ МЕТОДОМ ВРЕМЯПРОЛЕТНОЙ МАСС-СПЕКТРОМЕТРИИ","authors":"Д. В. Ульшина, Е. И. Еременко, Дмитрий Александрович Ковалев, Алла Рязанова, Ирина Валерьевна Кузнецова, Л. Ю. Аксенова, О. В. Семенова, О. В. Бобрышева, Ю. В. Сирица, Александр Куличенко","doi":"10.36233/0372-9311-2018-6-66-72","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-66-72","url":null,"abstract":"Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72559677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-28DOI: 10.36233/0372-9311-2018-6-80-85
А. Г. Драгомерецкая, Ольга Троценко, Л. А. Бебенина, Ольга Курганова, М. Е. Игнатьева, Татьяна Зайцева, П. В. Копылов, Д. В. Маслов, Н. И. Жданова, А. А. Рубцова, О. А. Фунтусова, И. Ю. Феделеш
Aim. To analyze current epidemiological and epizootological situation on cystic (hydatidiform) echinococcosis in constituent entities of the Far Eastern Federal District (FEFD) of the Russian Federation. Materials and methods. Data of the administrations of the Federal service for surveillance on consumers rights protection and human wellbeing as well as data of the FBIH «Center of hygiene and epidemiology» located in different constituent territories of the FEFD during 2013-2017 was used. A review of scientific papers concerning the topic of the current article was performed. Results. Mainly sporadic cases of the disease were registered in 7 out of 9 constituent entities of the FEFD during the 2013-2017. The Jewish Autonomous district and Kamchatka region were free of the echinococcosis during the 5-year observation period. Echinococcosis cases were registered annually in the Chukotka Autonomous district, Republic Sakha (Yakutia) and Primorsky region. Herewith, the incidence rates in Chukotka Autonomous district and Republic Sakha (Yakutia) notably exceeded the national average. No fatal outcomes due to cystic echinococcosis in the FEFD were registered. Conclusion. Implementation of immunoepidemiological surveillance of the population of different age groups and professions is recommended on the constituent entities with registered cases of cystic echinococcosis.
{"title":"ЦИСТНЫЙ ЭХИНОКОККОЗ В ДАЛЬНЕВОСТОЧНОМ ФЕДЕРАЛЬНОМ ОКРУГЕ: СОВРЕМЕННОЕ СОСТОЯНИЕ ПРОБЛЕМЫ","authors":"А. Г. Драгомерецкая, Ольга Троценко, Л. А. Бебенина, Ольга Курганова, М. Е. Игнатьева, Татьяна Зайцева, П. В. Копылов, Д. В. Маслов, Н. И. Жданова, А. А. Рубцова, О. А. Фунтусова, И. Ю. Феделеш","doi":"10.36233/0372-9311-2018-6-80-85","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-80-85","url":null,"abstract":"Aim. To analyze current epidemiological and epizootological situation on cystic (hydatidiform) echinococcosis in constituent entities of the Far Eastern Federal District (FEFD) of the Russian Federation. Materials and methods. Data of the administrations of the Federal service for surveillance on consumers rights protection and human wellbeing as well as data of the FBIH «Center of hygiene and epidemiology» located in different constituent territories of the FEFD during 2013-2017 was used. A review of scientific papers concerning the topic of the current article was performed. Results. Mainly sporadic cases of the disease were registered in 7 out of 9 constituent entities of the FEFD during the 2013-2017. The Jewish Autonomous district and Kamchatka region were free of the echinococcosis during the 5-year observation period. Echinococcosis cases were registered annually in the Chukotka Autonomous district, Republic Sakha (Yakutia) and Primorsky region. Herewith, the incidence rates in Chukotka Autonomous district and Republic Sakha (Yakutia) notably exceeded the national average. No fatal outcomes due to cystic echinococcosis in the FEFD were registered. Conclusion. Implementation of immunoepidemiological surveillance of the population of different age groups and professions is recommended on the constituent entities with registered cases of cystic echinococcosis.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79228701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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