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Иммунологические и эпидемиологические аспекты иммуногенности капсульного полисахарида Streptococcus pneumoniae серотипа 3 в составе пневмококковых вакцин
Q3 Medicine Pub Date : 2020-03-06 DOI: 10.36233/0372-9311-2020-97-1-72-82
A. Y. Zaitsev, E. A. Kurbatova, N. B. Egorova, E. V. Sukhova, Nikolay E. Nifantiev
The introduction of pneumococcal vaccines into national immunization programmes around the world has reduced the incidence of pneumococcal vaccine serotypes, but had no influence on the incidence of Streptococcus pneumoniae serotype 3 included in their composition. The results of evaluation of epidemiological efficacy and immunogenicity of capsular polysaccharide of S. pneumoniae serotype 3 capsular polysaccharide (CP) in conjugated and polysaccharide pneumococcal vaccines are contradictory. Some studies have shown the effectiveness of vaccination, other studies indicate insufficient immunogenicity and prophylactic efficacy of S. pneumoniae serotype 3 CP. The authors’ analysis of the results of clinical studies showed that the prophylactic efficacy of S. pneumoniae serotype 3 CP depends on the type of vaccine, nosological form of the disease, age, immunization schedule. According to the literature data, the most informative parameter of the protective activity of S. pneumoniae CP in pneumococcal vaccines, including serotype 3, is opsonophagocytosis. The experimental data of the low immunogenicity of serotype 3 CP, presumably associated with an unusual way of synthesis of its CP, are considered. To increase the im muno genicity of S. pneumoniae serotype 3 CP, the use of synthetic oligosaccharides of a strictly defined chemical structure corresponding to the protective fragments of serotype 3 CP and conjugated with a carrier protein for induction of T-dependent immune response and immunological memory is promising.
将肺炎球菌疫苗引入世界各地的国家免疫规划,减少了肺炎球菌疫苗血清型的发病率,但对其成分中包含的肺炎链球菌血清3型的发病率没有影响。肺炎链球菌血清3型荚膜多糖(CP)在共轭肺炎球菌疫苗和多糖肺炎球菌疫苗中的流行病学疗效和免疫原性评价结果相互矛盾。一些研究显示疫苗接种的有效性,另一些研究表明3型肺炎链球菌的免疫原性和预防效果不足。作者对临床研究结果的分析表明,3型肺炎链球菌的预防效果取决于疫苗的类型、疾病的病原学形式、年龄、免疫接种计划。根据文献资料,肺炎链球菌CP在肺炎球菌疫苗(包括血清3型)中的保护活性最具信息量的参数是调理吞噬作用。考虑到血清3型CP低免疫原性的实验数据,可能与合成其CP的一种不寻常的方式有关。为了提高肺炎链球菌血清3型CP的免疫原性,利用与血清3型CP的保护片段具有严格定义的化学结构并与载体蛋白偶联的低聚糖来诱导t依赖性免疫应答和免疫记忆是有希望的。
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引用次数: 3
Пространственная визуализация данных по антибиотикорезистентности штаммов холерных вибрионов, изолированных на территории России 在俄罗斯境内孤立的霍乱病毒菌株的抗生素活性数据空间可视化
Q3 Medicine Pub Date : 2020-03-06 DOI: 10.36233/0372-9311-2020-97-1-47-54
N. A. Selyanskaya, E. A. Bereznyak, A. V. Trishina, I. R. Simonova, L. A. Egiazaryan, A. S. Vodopyanov
Aim . The aim was to develop an integrated online and updated geographic information system (GIS) for the systematiza tion and analysis of information on V. cholerae El Tor antibiotic resistance. Material and methods . The method of serial dilutions in a dense nutrient medium (MUK 4.2.2495-09) was used to determine the sensitivity/resistance to 22 antibacterial preparations of V. cholerae O1 El Tor strains isolated from people and from aquatic environmental objects in Russia in 2005–2016. The development of the Internet version of the GIS was carried out using programming languages HTML, JavaScript and PHP and cartographic data obtained from the corporation Rostelecom (Russia) and the community OpenStreetMap. Results . A comparative analysis of the antibiotic resistance of the strains by regions of the Russian Federation and by isolation time showed an increase of strains resistant to streptomycin, ampicillin, rifampicin and furazolidone, the appearance of cultures resistant to nalidixic acid and ceftriaxone, reduced resistance to trimethoprim/sulfamethoxazole in 2012–2016 compared to 2005–2009. In the Stavropol Krai, the isolated V. cholerae O1 El Tor were characterized by resistance to furazolidone (33.3%) and trimethoprim/sulfamethoxazole (100%); in the Primorsky Krai — to ampicillin, streptomycin, rifampicin (7%), furazolidone (43%), trimethoprim/sulfamethoxazole (100%); in the Irkutsk region and Kalmykia, to ampicillin (8.3% and 11%), furazolidone and trimethoprim/sulfamethoxazole (11% and 89%). Conclusion . The GIS developed allows to collect and analyze information on the antibiotic resistance of V. cholerae O1 El Tor, and to select the strains for given properties.
的目标。目的是开发一个综合的在线和更新的地理信息系统(GIS),以便系统化和分析关于霍乱弧菌El - Tor抗生素耐药性的信息。材料和方法。采用密集营养培养基(MUK 4.2.2495-09)连续稀释法测定2005-2016年从俄罗斯人群和水生环境物分离的霍乱弧菌O1 El - Tor菌株对22种抗菌制剂的敏感性/耐药性。互联网版GIS的开发使用了HTML、JavaScript和PHP编程语言,以及从Rostelecom(俄罗斯)公司和OpenStreetMap社区获得的地图数据。结果。俄罗斯联邦各地区和分离时间菌株耐药性对比分析显示,与2005-2009年相比,2012-2016年对链霉素、氨苄西林、利福平和呋喃唑酮耐药菌株增加,对萘啶酸和头孢曲松耐药菌株出现,对甲氧苄啶/磺胺甲恶唑耐药降低。斯塔夫罗波尔地区分离的霍乱弧菌o1el - Tor对呋喃唑酮(33.3%)和甲氧苄啶/磺胺甲恶唑(100%)耐药;在滨海边疆区-氨苄西林、链霉素、利福平(7%)、呋喃唑酮(43%)、甲氧苄啶/磺胺甲恶唑(100%);在伊尔库茨克地区和卡尔梅克,分别为氨苄西林(8.3%和11%)、呋喃唑酮和甲氧苄啶/磺胺甲恶唑(11%和89%)。结论。开发的地理信息系统允许收集和分析霍乱弧菌O1 El - Tor的抗生素耐药性信息,并根据给定的特性选择菌株。
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引用次数: 1
ТЕОРИЯ САПРОНОЗНЫХ ИНФЕКЦИЙ: ИСТОРИЯ РАЗВИТИЯ И ПУТИ СОВЕРШЕНСТВОВАНИЯ В СИСТЕМЕ МЕДИКО-БИОЛОГИЧЕСКИХ НАУК
Q3 Medicine Pub Date : 2020-03-06 DOI: 10.36233/0372-9311-2020-97-1-91-101
A. Belov, A. Panin
The article represents the history of studies of human diseases caused by faсultative parasites of humans, animals and plants, attributed by microbiologist V.I. Terskych in 1958 to the third class of infections named sapronoses. The concept of sapronous pathogens ecology is one of priorities of Russian medicine. Since that time, facts and generalizations have been accumulated in the world and national science, allowing to revise the initial concept of sapronous infections of biota — from prokaryotes, plants and invertebrates to warm-blooded animals and humans. To decide the controversial issues of the sapronoses theory, epidemiological terminology and systematics of infectious pathology, it is necessary to integrate the knowledge and systemic participation of specialists in medicine and biology in studying this problem. The results of this cooperation will make it possible to clarify and form a unified theoretical concept of the population pathology of the biota based on the ecological approach and general biological regularities of life existence in the world.
这篇文章叙述了由人类、动物和植物的繁殖性寄生虫引起的人类疾病的研究历史,微生物学家V.I. Terskych在1958年将其归因于第三类感染,称为皂素病。sapronous病原菌生态学的概念是俄罗斯医学的重点之一。从那时起,世界和国家科学积累了事实和概括,允许修改生物群-从原核生物,植物和无脊椎动物到温血动物和人类-的sapronous感染的最初概念。要解决感染病理学中存在争议的皂素理论、流行病学术语和系统分类等问题,需要整合医学和生物学专家的知识和系统参与。这种合作的结果将有可能在生态学方法和世界上生命存在的一般生物学规律的基础上,阐明和形成一个统一的生物群种群病理的理论概念。
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引用次数: 5
Модель профилактической эффективности противогриппозной вакцины с поправкой на гипотезу антигенного расстояния 反流感疫苗预防效率模型,修正抗原距离假设
Q3 Medicine Pub Date : 2020-03-05 DOI: 10.36233/0372-9311-2020-97-1-15-18
Игорь Дмитриевич Колесин, Екатерина Михайловна Житкова
The aim of the study is to find a quantitative relationship between antigenic distances (AD) and vaccination effectiveness (VE) and investigate the response of VE to changes in AD. Material and methods . Through the epidemiological data of three influenza seasons in Canada, interpreted within the framework of the antigenic distance hypothesis (ADH), the introduction of the correction factor into the model for estimating VE was substantiated considering the antigenic relationship between the previous season vaccine (V1), the current season vaccine (V2) and the circulating epidemic strain (e). Results . A quantitative relationship between VE and AD was found, reproducing the results of epidemiological observation of two groups of people: vaccinated in the previous and current seasons (V1+V2) and vaccinated only in the current season (0+V2). The difference in the response of VE to different indicators of AD was found and allowing to use only one significant indicator. Conclusion . Тhe model proposed relates the microbiological indicators AD with epidemiological characteristic of VE. The model extends the analysis, allowing to use it as an assessment tool for the expected changes in vaccine effectiveness in various settings of the ADH hypothesis experiment.
本研究旨在寻找抗原距离(antigenic distance, AD)与疫苗接种有效性(vaccine effectiveness, VE)之间的定量关系,并探讨VE对AD变化的响应。材料和方法。通过加拿大三个流感季节的流行病学数据,在抗原距离假设(ADH)的框架下进行解释,考虑到前一季疫苗(V1)、当前季疫苗(V2)与流行毒株(e)之间的抗原关系,验证了将校正因子引入VE估计模型的合理性。发现了VE与AD之间的定量关系,再现了两组人群的流行病学观察结果:在上一季和当前季节接种疫苗(V1+V2)和仅在当前季节接种疫苗(0+V2)。发现VE对AD不同指标的反应存在差异,并且只允许使用一个显著指标。结论。Тhe模型将微生物学指标AD与VE的流行病学特征联系起来。该模型扩展了分析,允许将其用作评估工具,用于在ADH假设实验的各种设置中疫苗有效性的预期变化。
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引用次数: 0
[Argentine hemorrhagic fever]. [阿根廷出血热]。
Q3 Medicine Pub Date : 2020-02-10 DOI: 10.32388/f9dtvh
V. A. Markin, V. B. Pantiukhov, V. I. Markov, V. Bondarev
Features of the Argentine hemorrhagic fever are described in the review. Epidemiology, etiology, clinical presentation and pathogenesis of the disease are examined. Special consideration is given to the features of the pathological agent of Argentine hemorrhagic fever--the Junin virus. Features of the disease diagnostics are indicated--virological and serological studies, immunochemical and molecular-biological methods of identification of the pathological agent and antibodies against it. Approaches to etiotropic therapy of this disease and vaccination are examined. Based on the foreign experience perspective guidance for the creation of the system of protection of the population of the Russian Federation against Argentine hemorrhagic fever are presented.
本文描述了阿根廷出血热的特点。对该病的流行病学、病因学、临床表现和发病机制进行了检查。特别考虑到阿根廷出血热的病理因子——朱宁病毒的特点。指出了疾病诊断的特点——病毒学和血清学研究,免疫化学和分子生物学方法鉴定病理因子和抗体。探讨了本病的病因治疗和疫苗接种方法。在国外经验的基础上,提出了建立俄罗斯联邦人口预防阿根廷出血热保护制度的指导意见。
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引用次数: 1
Изменение спектра жирных кислот как один из механизмов адаптации/персистенции микроорганизмов
Q3 Medicine Pub Date : 2019-11-19 DOI: 10.36233/0372-9311-2019-5-109-118
Е. С. Шипко, О. В. Дуванова
The review presents the basic information available in the literature on changes in the composition of fatty acids in various microorganisms in response to various environmental factors (stressors). The issues affecting the importance of fatty acids as biomarkers of pathogenetic and adaptive-persistent potential of bacteria are discussed. The prospects of studying the spectrum of fatty acids in the field of biochemistry, in particular, lipidomics of infectious diseases are noted.
本文综述了在各种环境因素(应激源)作用下,各种微生物脂肪酸组成变化的文献中的基本信息。本文讨论了影响脂肪酸作为细菌致病和适应持续潜力生物标志物重要性的问题。指出了脂肪酸谱研究在生物化学领域,特别是传染病脂质组学研究中的前景。
{"title":"Изменение спектра жирных кислот как один из механизмов адаптации/персистенции микроорганизмов","authors":"Е. С. Шипко, О. В. Дуванова","doi":"10.36233/0372-9311-2019-5-109-118","DOIUrl":"https://doi.org/10.36233/0372-9311-2019-5-109-118","url":null,"abstract":"The review presents the basic information available in the literature on changes in the composition of fatty acids in various microorganisms in response to various environmental factors (stressors). The issues affecting the importance of fatty acids as biomarkers of pathogenetic and adaptive-persistent potential of bacteria are discussed. The prospects of studying the spectrum of fatty acids in the field of biochemistry, in particular, lipidomics of infectious diseases are noted.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"6 1","pages":"109-118"},"PeriodicalIF":0.0,"publicationDate":"2019-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78564586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
[Bacterial vaginosis]. [细菌性阴道病]。
Q3 Medicine Pub Date : 2019-01-01 DOI: 10.1891/9780826195197.0060
E. N. Torganova, S. A. Abdullaeva, M. Boichenko
{"title":"[Bacterial vaginosis].","authors":"E. N. Torganova, S. A. Abdullaeva, M. Boichenko","doi":"10.1891/9780826195197.0060","DOIUrl":"https://doi.org/10.1891/9780826195197.0060","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"4 1","pages":"100-3"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44557742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ РЕКОМБИНАНТНОЙ ВАКЦИНЫ СИНЕГНОЙНОЙ
Q3 Medicine Pub Date : 2018-12-28 DOI: 10.36233/0372-9311-2018-6-31-37
N. Mihailova, A. A. Kaloshin, E. Zimina, A. Soldatenkova, A. V. Poddubikov
Aim. Evaluation of the efficacy and safety of the three experimental lots of Recombinant Pseudomonas Vaccine. Materials and methods. The preparation contained the recombinant proteins OprF and toxoid that were purified by chromatography in nickel-sepharose. Aluminum hydroxide was used as an adjuvant. The authenticity of the vaccine components was confirmed by electrophoresis and immunoblotting. The concentration of endotoxin in the vaccine was determined by LAL test. The abnormal toxicity was evaluated in mice and cavy. The anaphylactic activity was evaluated in cavy. The delayed-type hypersensitivity reaction was evaluated in mice. Evaluation of the immunogenicity was carried out in an experiment with on double immunization of mice with following intraperitoneally infection by a live virulent culture of P. aeruginosa (PA-103 strain). Results. The authenticity of the vaccine, sterility, non-pyrogenicity and non-toxicity were confirmed after the studying of the quality indicators of the three lots of Recombinant Pseudomonas Vaccine. In animal experiments, the vaccine did not possess allergic properties and it was shown that it protected mice against Pseudomonas infection with Index of efficiency 3.0 and more. Conclusion. The efficacy, the safety, and the standardization of three experimental lots of the recombinant vaccine, which is intended to prevent infections caused by P. aeruginosa, have been shown.
的目标。重组假单胞菌疫苗三个实验批次的有效性和安全性评价。材料和方法。该制剂含有重组蛋白OprF和类毒素,经镍-sepharose层析纯化。氢氧化铝作为佐剂。通过电泳和免疫印迹验证了疫苗成分的真实性。采用LAL法测定疫苗内毒素浓度。对小鼠和家鼠进行了异常毒性评价。对豚鼠进行过敏反应活性评价。观察小鼠的延迟型超敏反应。采用铜绿假单胞菌(PA-103株)活毒力培养物对小鼠腹腔感染后的双重免疫实验,对其免疫原性进行了评价。结果。通过对三批重组假单胞菌疫苗质量指标的研究,证实了疫苗的真实性、无菌性、无热原性和无毒性。动物实验表明,该疫苗不具有过敏特性,对假单胞菌感染的保护作用指数达到3.0以上。结论。三批用于预防铜绿假单胞菌感染的重组疫苗的有效性、安全性和标准化已得到证实。
{"title":"ДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ РЕКОМБИНАНТНОЙ ВАКЦИНЫ СИНЕГНОЙНОЙ","authors":"N. Mihailova, A. A. Kaloshin, E. Zimina, A. Soldatenkova, A. V. Poddubikov","doi":"10.36233/0372-9311-2018-6-31-37","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-31-37","url":null,"abstract":"Aim. Evaluation of the efficacy and safety of the three experimental lots of Recombinant Pseudomonas Vaccine. Materials and methods. The preparation contained the recombinant proteins OprF and toxoid that were purified by chromatography in nickel-sepharose. Aluminum hydroxide was used as an adjuvant. The authenticity of the vaccine components was confirmed by electrophoresis and immunoblotting. The concentration of endotoxin in the vaccine was determined by LAL test. The abnormal toxicity was evaluated in mice and cavy. The anaphylactic activity was evaluated in cavy. The delayed-type hypersensitivity reaction was evaluated in mice. Evaluation of the immunogenicity was carried out in an experiment with on double immunization of mice with following intraperitoneally infection by a live virulent culture of P. aeruginosa (PA-103 strain). Results. The authenticity of the vaccine, sterility, non-pyrogenicity and non-toxicity were confirmed after the studying of the quality indicators of the three lots of Recombinant Pseudomonas Vaccine. In animal experiments, the vaccine did not possess allergic properties and it was shown that it protected mice against Pseudomonas infection with Index of efficiency 3.0 and more. Conclusion. The efficacy, the safety, and the standardization of three experimental lots of the recombinant vaccine, which is intended to prevent infections caused by P. aeruginosa, have been shown.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"43 1","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86448021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ВЫЯВЛЕНИЕ ОСОБЕННОСТЕЙ МАСС-СПЕКТРОВ БЕЛКОВЫХ ЭКСТРАКТОВ СПОРОВОЙ И ВЕГЕТАТИВНОЙ ФОРМ ВОЗБУДИТЕЛЯ СИБИРСКОЙ ЯЗВЫ МЕТОДОМ ВРЕМЯПРОЛЕТНОЙ МАСС-СПЕКТРОМЕТРИИ 通过时间质谱仪检测出孢子和植物人炭疽病诱因的质谱特征
Q3 Medicine Pub Date : 2018-12-28 DOI: 10.36233/0372-9311-2018-6-66-72
Д. В. Ульшина, Е. И. Еременко, Дмитрий Александрович Ковалев, Алла Рязанова, Ирина Валерьевна Кузнецова, Л. Ю. Аксенова, О. В. Семенова, О. В. Бобрышева, Ю. В. Сирица, Александр Куличенко
Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.
的目标。利用Mass-Up程序资源和开放源代码r的统计软件包,采用MALDI-TOF质谱法对炭疽菌孢子和营养形态的蛋白质谱特征进行了研究。斯塔夫罗波尔鼠疫控制研究所微生物标本中32株炭疽芽胞杆菌的孢子和营养形态,其中包括2016年亚马尔炭疽疫情中分离的8株菌株。蛋白质谱分析在Microflex MALDI-TOF质谱仪«Bruker Daltonics»上进行。结果。另一种生物信息统计方法用于分析炭疽病原体的MALDI-TOF质谱,使得根据识别相应的生物标志物组来区分微生物的孢子和营养形式成为可能。结论。利用MALDI-TOF质谱对炭疽病原体典型菌株和非典型菌株的营养细胞和孢子细胞进行了比较。实验证实,芽孢和营养形式的炭疽芽孢杆菌培养物的蛋白质谱存在显著差异,这种差异可用于寻找每种形式的潜在标记物。
{"title":"ВЫЯВЛЕНИЕ ОСОБЕННОСТЕЙ МАСС-СПЕКТРОВ БЕЛКОВЫХ ЭКСТРАКТОВ СПОРОВОЙ И ВЕГЕТАТИВНОЙ ФОРМ ВОЗБУДИТЕЛЯ СИБИРСКОЙ ЯЗВЫ МЕТОДОМ ВРЕМЯПРОЛЕТНОЙ МАСС-СПЕКТРОМЕТРИИ","authors":"Д. В. Ульшина, Е. И. Еременко, Дмитрий Александрович Ковалев, Алла Рязанова, Ирина Валерьевна Кузнецова, Л. Ю. Аксенова, О. В. Семенова, О. В. Бобрышева, Ю. В. Сирица, Александр Куличенко","doi":"10.36233/0372-9311-2018-6-66-72","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-66-72","url":null,"abstract":"Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"67 4","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72559677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
ЦИСТНЫЙ ЭХИНОКОККОЗ В ДАЛЬНЕВОСТОЧНОМ ФЕДЕРАЛЬНОМ ОКРУГЕ: СОВРЕМЕННОЕ СОСТОЯНИЕ ПРОБЛЕМЫ
Q3 Medicine Pub Date : 2018-12-28 DOI: 10.36233/0372-9311-2018-6-80-85
А. Г. Драгомерецкая, Ольга Троценко, Л. А. Бебенина, Ольга Курганова, М. Е. Игнатьева, Татьяна Зайцева, П. В. Копылов, Д. В. Маслов, Н. И. Жданова, А. А. Рубцова, О. А. Фунтусова, И. Ю. Феделеш
Aim. To analyze current epidemiological and epizootological situation on cystic (hydatidiform) echinococcosis in constituent entities of the Far Eastern Federal District (FEFD) of the Russian Federation. Materials and methods. Data of the administrations of the Federal service for surveillance on consumers rights protection and human wellbeing as well as data of the FBIH «Center of hygiene and epidemiology» located in different constituent territories of the FEFD during 2013-2017 was used. A review of scientific papers concerning the topic of the current article was performed. Results. Mainly sporadic cases of the disease were registered in 7 out of 9 constituent entities of the FEFD during the 2013-2017. The Jewish Autonomous district and Kamchatka region were free of the echinococcosis during the 5-year observation period. Echinococcosis cases were registered annually in the Chukotka Autonomous district, Republic Sakha (Yakutia) and Primorsky region. Herewith, the incidence rates in Chukotka Autonomous district and Republic Sakha (Yakutia) notably exceeded the national average. No fatal outcomes due to cystic echinococcosis in the FEFD were registered. Conclusion. Implementation of immunoepidemiological surveillance of the population of different age groups and professions is recommended on the constituent entities with registered cases of cystic echinococcosis.
的目标。目的分析俄罗斯联邦远东联邦区(FEFD)各组成实体囊性(包虫病)棘球蚴病流行病学和流行病学现状。材料和方法。使用了2013-2017年期间联邦消费者权利保护和人类福利监督局的数据,以及联邦卫生和流行病学中心在联邦联邦不同组成地区的数据。对有关本文主题的科学论文进行了回顾。结果。在2013-2017年期间,FEFD的9个组成实体中有7个登记了主要散发病例。在5年的观察期内,犹太自治区和堪察加地区无棘球蚴病。每年在楚科奇自治区、萨哈共和国(雅库特)和滨海边疆区登记棘球蚴病病例。其中,楚科奇自治区和萨哈共和国(雅库特)的发病率明显超过全国平均水平。在FEFD中没有因囊性包虫病而死亡的结果。结论。建议对有囊性包虫病登记病例的组成实体实施不同年龄组和职业人群的免疫流行病学监测。
{"title":"ЦИСТНЫЙ ЭХИНОКОККОЗ В ДАЛЬНЕВОСТОЧНОМ ФЕДЕРАЛЬНОМ ОКРУГЕ: СОВРЕМЕННОЕ СОСТОЯНИЕ ПРОБЛЕМЫ","authors":"А. Г. Драгомерецкая, Ольга Троценко, Л. А. Бебенина, Ольга Курганова, М. Е. Игнатьева, Татьяна Зайцева, П. В. Копылов, Д. В. Маслов, Н. И. Жданова, А. А. Рубцова, О. А. Фунтусова, И. Ю. Феделеш","doi":"10.36233/0372-9311-2018-6-80-85","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-6-80-85","url":null,"abstract":"Aim. To analyze current epidemiological and epizootological situation on cystic (hydatidiform) echinococcosis in constituent entities of the Far Eastern Federal District (FEFD) of the Russian Federation. Materials and methods. Data of the administrations of the Federal service for surveillance on consumers rights protection and human wellbeing as well as data of the FBIH «Center of hygiene and epidemiology» located in different constituent territories of the FEFD during 2013-2017 was used. A review of scientific papers concerning the topic of the current article was performed. Results. Mainly sporadic cases of the disease were registered in 7 out of 9 constituent entities of the FEFD during the 2013-2017. The Jewish Autonomous district and Kamchatka region were free of the echinococcosis during the 5-year observation period. Echinococcosis cases were registered annually in the Chukotka Autonomous district, Republic Sakha (Yakutia) and Primorsky region. Herewith, the incidence rates in Chukotka Autonomous district and Republic Sakha (Yakutia) notably exceeded the national average. No fatal outcomes due to cystic echinococcosis in the FEFD were registered. Conclusion. Implementation of immunoepidemiological surveillance of the population of different age groups and professions is recommended on the constituent entities with registered cases of cystic echinococcosis.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"74 1","pages":"80-85"},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79228701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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