Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-52-57
M. P. Chervakova, T. Sharov, L. P. Barkova, A. Barkov, D. Viktorov, A. Toporkov
Aim. Identification of obtained in host-simulated conditions immunogenic proteins of isogenic variants of Bacillus anthracis 575/122. Materials and methods. We used culture filtrate of isogenic variants of B. anthracis 575/122: R02 (pXOL pXO2 + ); R01 (pXO1 + pXO2‘); R00 (pXOL pX02~), obtained in host-simulated conditions. In the one-dimensional polyacrylamide gel electrophoresis and immunoblotting with hyperimmune serums immunodominant proteins, that have been identified in MALDI TOF MS. Results. Immunoblotting revealed proteins with molecular masses in range 97 - 14.1 kDa. 90 kDa protein from strain B. anthracis 575/122 R01 in MALDI TOF MS was identified as protective antigen with 85.810 kDa. Protein with molecular mass 60 kDa was identified as GMP synthase with molecular mass 57.239 kDa. In the culture filtrates of three strains two common antigen were identified: protein with molecular mass 97 kDa, identified as B. anthracis EA 1 with molecular mass 91.361 kDa protein and 45 kDa protein as enolase B. anthracis with molecular mass 46.418 kDa. Conclusion. Thus, the conditions that simulate the host can promote the production of immunodominant proteins of B. anthracis. The data about molecular-weight characteristics of protective antigen and EA 1 protein as well as some of proteases of B. anthracis are confirmed by the MALDI TOF MS. The results can be used for isolation of these proteins to improve the diagnostic and vaccine preparations.
{"title":"ИДЕНТИФИКАЦИЯ ИММУНОГЕННЫХ БЕЛКОВ ШТАММОВ BACILLUS ANTHRACIS В MALDITOF MS","authors":"M. P. Chervakova, T. Sharov, L. P. Barkova, A. Barkov, D. Viktorov, A. Toporkov","doi":"10.36233/0372-9311-2018-1-52-57","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-52-57","url":null,"abstract":"Aim. Identification of obtained in host-simulated conditions immunogenic proteins of isogenic variants of Bacillus anthracis 575/122. Materials and methods. We used culture filtrate of isogenic variants of B. anthracis 575/122: R02 (pXOL pXO2 + ); R01 (pXO1 + pXO2‘); R00 (pXOL pX02~), obtained in host-simulated conditions. In the one-dimensional polyacrylamide gel electrophoresis and immunoblotting with hyperimmune serums immunodominant proteins, that have been identified in MALDI TOF MS. Results. Immunoblotting revealed proteins with molecular masses in range 97 - 14.1 kDa. 90 kDa protein from strain B. anthracis 575/122 R01 in MALDI TOF MS was identified as protective antigen with 85.810 kDa. Protein with molecular mass 60 kDa was identified as GMP synthase with molecular mass 57.239 kDa. In the culture filtrates of three strains two common antigen were identified: protein with molecular mass 97 kDa, identified as B. anthracis EA 1 with molecular mass 91.361 kDa protein and 45 kDa protein as enolase B. anthracis with molecular mass 46.418 kDa. Conclusion. Thus, the conditions that simulate the host can promote the production of immunodominant proteins of B. anthracis. The data about molecular-weight characteristics of protective antigen and EA 1 protein as well as some of proteases of B. anthracis are confirmed by the MALDI TOF MS. The results can be used for isolation of these proteins to improve the diagnostic and vaccine preparations.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"19 1","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84392106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-17-23
N. Briko, L. R. Batyrshina, A. Briko
Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor bidity and economic loss in the country had been processed. According to the official statistics in laboring are within the risk group for pneumonia. Data source for the cost of the healthcare for disease had been the compulsory health insurance system 2016 state tariffs, for the vaccination cost results of the PCV13 procurement bidding had been used. Results. Data extrapolation from national and international studies to the cohort of individuals with chronic respiratory diseases, cardiovascular diseases and diabetes mellitus vaccinated against pneumococcal infection showed a significant decrease in the risk of un derlying diseases complications (OP=0,58, p<0,05), hospitalizations number (OP=0,02, p<0,05) and expected mortality. The cost of vaccination in the evaluated group of patients was 25 869.5 min RUB. According to the modeling results PCV13 use will allow to statistically significantly decrease the number of relapses and hospitalizations that will permit to save up to 14 359.9 min rubles annually. Thus, in the two-year horizon, the total fiscal savings will amount to 2 850.30 million RUB and at least 61 702 of the patients' lives retained in a 5-year term with a single dose PCV13 administration. Conclusion. The study results indicate potential high epidemiological and clinical effectiveness of pneumococcal vaccination of the laboring males suffering from chronic diseases. Vaccination as a cost-effective investment in healthcare creates the opportunity of reduc tions in morbidity, number of exacerbations, hospitalization rate and mortality in the vaccinated cohort.
的目标。各种慢性疾病的劳动男性接种肺炎球菌疫苗的流行病学和经济效果的预后研究。材料和方法。在预测(马尔可夫)模型中，基于已发表的数据，评估了5年期劳动男性肺炎球菌疫苗在降低死亡率、避免发病率和经济损失方面的有效性。根据官方统计，产妇属于患肺炎的高危人群。疾病医疗费用的数据来源一直是强制性医疗保险制度2016年国家关税，用于疫苗接种费用的PCV13采购招标结果一直使用。结果。对慢性呼吸系统疾病、心血管疾病和糖尿病患者接种肺炎球菌感染疫苗的国家和国际研究数据外推表明，接种肺炎球菌疫苗可显著降低潜在疾病并发症的风险(OP=0,58, p< 0.05)、住院次数(OP=0,02, p< 0.05)和预期死亡率。评估组患者的疫苗接种费用为25 869.5 min RUB。根据建模结果，PCV13的使用将在统计上显著减少复发和住院人数，每年可节省高达14 359.9分卢布。因此，在两年的期限内，总财政节省将达到285030万卢布，并且在单剂PCV13给药的5年期限内至少保留61702名患者的生命。结论。研究结果提示慢性疾病劳动男性肺炎球菌疫苗接种可能具有较高的流行病学和临床效果。疫苗接种作为一项具有成本效益的卫生保健投资，为降低接种人群的发病率、恶化次数、住院率和死亡率创造了机会。
{"title":"EVALUATION OF THE PROGNOSTIC EPIDEMIOLOGICAL AND ECONOMIC EFFICACY OF VACCINATION AGAINST PNEUMOCOCCAL INFECTION IN MEN OF WORKING AGE WITH VARIOUS CHRONIC DISEASES","authors":"N. Briko, L. R. Batyrshina, A. Briko","doi":"10.36233/0372-9311-2018-1-17-23","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-17-23","url":null,"abstract":"Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor bidity and economic loss in the country had been processed. According to the official statistics in laboring are within the risk group for pneumonia. Data source for the cost of the healthcare for disease had been the compulsory health insurance system 2016 state tariffs, for the vaccination cost results of the PCV13 procurement bidding had been used. Results. Data extrapolation from national and international studies to the cohort of individuals with chronic respiratory diseases, cardiovascular diseases and diabetes mellitus vaccinated against pneumococcal infection showed a significant decrease in the risk of un derlying diseases complications (OP=0,58, p<0,05), hospitalizations number (OP=0,02, p<0,05) and expected mortality. The cost of vaccination in the evaluated group of patients was 25 869.5 min RUB. According to the modeling results PCV13 use will allow to statistically significantly decrease the number of relapses and hospitalizations that will permit to save up to 14 359.9 min rubles annually. Thus, in the two-year horizon, the total fiscal savings will amount to 2 850.30 million RUB and at least 61 702 of the patients' lives retained in a 5-year term with a single dose PCV13 administration. Conclusion. The study results indicate potential high epidemiological and clinical effectiveness of pneumococcal vaccination of the laboring males suffering from chronic diseases. Vaccination as a cost-effective investment in healthcare creates the opportunity of reduc tions in morbidity, number of exacerbations, hospitalization rate and mortality in the vaccinated cohort.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"16 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78311021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-10-16
N. Briko, E. Glushkova
Aim. To study the main epidemiological manifestations of GAS infection in Russia and to assess the scale of the problem. Materials and methods. A retrospective epidemiological analysis of official statistical reporting data (f. 2, f. 12) about the incidence and prevalence of the most sig nificant forms of group A streptococcal infection in 2009 — 2016 was performed. Results. There was a slight decrease in the total incidence of GAS infection in the Russian Federation. On the average, 1.8 million people fell ill every year, of which 600,000 were children aged 0 — 14. There was no reliable trend in incidence of acute rheumatic fever (ARF) in 2009 — 2016 (2.0 per 100 000 population). The risk group is adolescents. The main proportion is adults (64%). In recent years the incidence of rheumatic heart disease (RHD) increases rate in the adult group. Compared with the previous period (1996 — 2007), the situation with the incidence of diseases of the skin and subcutaneous tissue and prevalence of kidney disease improved. Conclusion. The findings indicate a high prevalence of streptococcal infection in the country. Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor bidity and economic loss in the country had been processed. According to the official statistics in
{"title":"STATUS AND TRENDS OF THE EPIDEMIC SITUATION OF GROUP A STREPTOCOCCAL (GAS) INFECTIONS IN RUSSIA IN RECENT YEARS","authors":"N. Briko, E. Glushkova","doi":"10.36233/0372-9311-2018-1-10-16","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-10-16","url":null,"abstract":"Aim. To study the main epidemiological manifestations of GAS infection in Russia and to assess the scale of the problem. Materials and methods. A retrospective epidemiological analysis of official statistical reporting data (f. 2, f. 12) about the incidence and prevalence of the most sig nificant forms of group A streptococcal infection in 2009 — 2016 was performed. Results. There was a slight decrease in the total incidence of GAS infection in the Russian Federation. On the average, 1.8 million people fell ill every year, of which 600,000 were children aged 0 — 14. There was no reliable trend in incidence of acute rheumatic fever (ARF) in 2009 — 2016 (2.0 per 100 000 population). The risk group is adolescents. The main proportion is adults (64%). In recent years the incidence of rheumatic heart disease (RHD) increases rate in the adult group. Compared with the previous period (1996 — 2007), the situation with the incidence of diseases of the skin and subcutaneous tissue and prevalence of kidney disease improved. Conclusion. The findings indicate a high prevalence of streptococcal infection in the country. Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor bidity and economic loss in the country had been processed. According to the official statistics in","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"86 1","pages":"10-16"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84368679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-101-111
E. V. Shubnikova, L. Merinova, T. Senina, E. V. Korol', O. Merinova
Обзор содержит данные литературы, касающиеся основных вопросов образования биопленок возбудителями мелиоидоза и сапа (Burkholderia pseudomallei и Burkholderia mal lei). Рассмотренароль известных структурных элементов клеток буркхольдерий (жгутиков, пилей IVтипа, ЛПС), а также белков-автотранспортеров адгезинов в прикреплении бак терий к поверхностям, формировании микроколоний и образовании биопленки. Представлены материалы исследования генетических регуляторных механизмов (QS-систем, RpoE-сигма фактора, c-di-GMP, двухкомпонентной системы трансдукции сигналов TCS) и данные о дифференциально экспрессирующихся генах, имеющих отношение к форми рованию биопленки В. pseudomallei. Приведены результаты изучения роли биопленок в вирулентности и резистентности к антибиотикам патогенных буркхольдерий и их значения в развитии хронических форм и рецидивирующего течения мелиоидоза и сапа.
这篇评论包含了有关梅里奥伊多萨和萨帕激发器生物胶片教育的基本问题的文献(Burkholderia pseudomallei和Burkholderia mal - lei)。研究了burkholdery细胞的已知结构元素(如引线、电锯、lps)，以及将油箱附着在表面、微生物群形成和生物胶片形成方面的附着蛋白。基因调节机制(QS-系统、RpoE- sigma因子、c-di-GMP、csi -GMP)和与b pseudomallei生物胶片的形式形成有关的微分表达基因。这些结果是研究生物胶片在毒性和耐药性方面的作用，以及它们在慢性形式和梅利奥伊多萨和萨帕复发方面的重要性。
{"title":"BIOFILMS OF PATHOGENIC BURKHOLDERIA AND THEIR ROLE IN RESISTANCE TO ANTIBIOTICS","authors":"E. V. Shubnikova, L. Merinova, T. Senina, E. V. Korol', O. Merinova","doi":"10.36233/0372-9311-2018-1-101-111","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-101-111","url":null,"abstract":"Обзор содержит данные литературы, касающиеся основных вопросов образования биопленок возбудителями мелиоидоза и сапа (Burkholderia pseudomallei и Burkholderia mal lei). Рассмотренароль известных структурных элементов клеток буркхольдерий (жгутиков, пилей IVтипа, ЛПС), а также белков-автотранспортеров адгезинов в прикреплении бак терий к поверхностям, формировании микроколоний и образовании биопленки. Представлены материалы исследования генетических регуляторных механизмов (QS-систем, RpoE-сигма фактора, c-di-GMP, двухкомпонентной системы трансдукции сигналов TCS) и данные о дифференциально экспрессирующихся генах, имеющих отношение к форми рованию биопленки В. pseudomallei. Приведены результаты изучения роли биопленок в вирулентности и резистентности к антибиотикам патогенных буркхольдерий и их значения в развитии хронических форм и рецидивирующего течения мелиоидоза и сапа.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"12 1","pages":"101-111"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78088719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-93-101
М. В. Полеева, Ольга Чемисова
Toxins - molecular weight compounds produced by microorganisms, animals, plants and possessing antigene properties. Recently due to the perceived threat of terrorist actions identification of a number of bacterial toxins is especially important. A new approach in the identification of toxins associated with the development of mass spectrometry and can be successfully used for analysis of most environmental toxins. The method of MALDI-MS allows the detection of toxins such as Shiga-toxin Escherichia coli, delta-toxin of Staphylococcus aureus, Bacillus cereus particular, botulinum neurotoxin, cholera toxin. Analytical and diagnostic characteristics of the method, the simplicity and speed studies indicate the long term implementation of a method in the practice of laboratory diagnostics in determining toxinproducing of the studied microorganisms.
{"title":"ИСПОЛЬЗОВАНИЕ МАСС-СПЕКТРОМЕТРИЧЕСКОГО АНАЛИЗА ДЛЯ ДЕТЕКЦИИ БАКТЕРИАЛЬНЫХ ТОКСИНОВ","authors":"М. В. Полеева, Ольга Чемисова","doi":"10.36233/0372-9311-2018-1-93-101","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-93-101","url":null,"abstract":"Toxins - molecular weight compounds produced by microorganisms, animals, plants and possessing antigene properties. Recently due to the perceived threat of terrorist actions identification of a number of bacterial toxins is especially important. A new approach in the identification of toxins associated with the development of mass spectrometry and can be successfully used for analysis of most environmental toxins. The method of MALDI-MS allows the detection of toxins such as Shiga-toxin Escherichia coli, delta-toxin of Staphylococcus aureus, Bacillus cereus particular, botulinum neurotoxin, cholera toxin. Analytical and diagnostic characteristics of the method, the simplicity and speed studies indicate the long term implementation of a method in the practice of laboratory diagnostics in determining toxinproducing of the studied microorganisms.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"38 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85923354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-2-30-38
A. Platonov, J. Koetsveld, N. Kolyasnikova, O. Stukolova, A. Dolgova, M. Toporkova, D. S. Sarksyan
Aim. Our aim was to study the bactericidal effect of human serum on Borrelia miyamotoi in vitro. Materials and methods. B. miyamotoi spirochetes (strains HT31 and LB-2001) were incu bated in non-immune serum of healthy donors (SHD) and in heat inactivated complement-de pleted SHD, as well as in serum samples of the patients recovered from ITBB-BM. The viability, that is motility, of borrelia after incubation was investigated by dark-field microscopy. The levels of serum antibody to Rmiyamofoi-specificproteins (GlpQ enzyme and four variable majorproteins Vlpl5/16, Vlpl8, Vspl, and Vlp5) were measured by specially designed plane protein microarray. Results. Borrelia Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals.
{"title":"BACTERICIDAL EFFECT OF HUMAN SERUM ON BORRELIA MIYAMOTOI, CAUSATIVE AGENT OF IXODES TICK-BORNE BORRELIOSIS (ITBB-BM)","authors":"A. Platonov, J. Koetsveld, N. Kolyasnikova, O. Stukolova, A. Dolgova, M. Toporkova, D. S. Sarksyan","doi":"10.36233/0372-9311-2018-2-30-38","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-30-38","url":null,"abstract":"Aim. Our aim was to study the bactericidal effect of human serum on Borrelia miyamotoi in vitro. Materials and methods. B. miyamotoi spirochetes (strains HT31 and LB-2001) were incu bated in non-immune serum of healthy donors (SHD) and in heat inactivated complement-de pleted SHD, as well as in serum samples of the patients recovered from ITBB-BM. The viability, that is motility, of borrelia after incubation was investigated by dark-field microscopy. The levels of serum antibody to Rmiyamofoi-specificproteins (GlpQ enzyme and four variable majorproteins Vlpl5/16, Vlpl8, Vspl, and Vlp5) were measured by specially designed plane protein microarray. Results. Borrelia Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"30-38"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83715715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-32-39
S. Svyatchenko, Durymanov Ag, I. Susloparov, N. Kolosova, N. I. Goncharova, O. Petrova, A. Epanchintseva, A. V. Maksyutova, K. Kondik, O. K. Slaboda, E. Ivanova, V. Mikheev, Ryzhikov Ab, T. Ilicheva
{"title":"SEVERE CASES OF SEASONAL INFLUENZA IN RUSSIA IN 2015 - 2016 AND 2016 - 2017","authors":"S. Svyatchenko, Durymanov Ag, I. Susloparov, N. Kolosova, N. I. Goncharova, O. Petrova, A. Epanchintseva, A. V. Maksyutova, K. Kondik, O. K. Slaboda, E. Ivanova, V. Mikheev, Ryzhikov Ab, T. Ilicheva","doi":"10.36233/0372-9311-2018-1-32-39","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-32-39","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"30 1","pages":"32-39"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76558925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-72-76
O. Vasileva, A. Volynkina, I. Kuznetsova, S. Pisarenko, A. Kulichenko
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Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-67-71
G. T. Uryadova, E. Gorelnikova, N. Fokina, A. S. Dolmashkina, L. Karpunina
Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals. Aim. Study of molecular-genetic properties of Shigella sonnei- 2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a genetic analyzer Ion Torrent Personal Genome Machine (PGM™) using fragment libraries (shot-gun). Aligning reads have been carried out with the program GS Reference Mapper. Results. Defined sequence — type of the studied strain — ST-152, one of the most common genotypes for S. sonnei. Demonstrated the high degree of similarity obtained contig to the sequences of the chromosome and plasmids А, В, С и E strains S. sonnei 53G and S. sonnei Ss046. Identified contigs with a high percentage similarity to the se quence of virulence plasmid p026-Vir of E. coli 026:H11
的目标。乳酸球菌外多糖(EPS)对体外吞噬金黄色葡萄球菌209-P小鼠巨噬细胞细胞因子活性影响的研究材料和方法。本研究采用了嗜热链球菌和乳酸乳球菌B-1662的EPS。在第1、3、5和7期分离AMP和PMP，体外模拟吞噬过程。在30分钟、1小时、6小时和24小时后，测定促炎细胞因子IL-1 a和TNF-a的含量。结果。eps对细胞因子的产生有不明确的影响。嗜热链球菌的EPS对合成的影响最大。结论。这项研究的结果使我们能够讨论利用嗜热链球菌的EPS作为一种预防性免疫调节剂来纠正动物细胞因子状态的可能性。的目标。2013年阿布哈兹共和国痢疾暴发期间分离的索内志贺氏菌- 2013株的分子遗传学特性研究材料和方法。用多位点序列分型(MLST)对被试菌株进行遗传分型。分析保守“管家”基因adk、fumC、icd、mdh、purA、recA、gyrB的核苷酸序列片段7。DNA片段序列与大肠杆菌MLST数据库中参考序列的比较。采用UPGMA方法和计算机程序start2进行系统发育分析。全基因组测序在基因分析仪Ion Torrent Personal Genome Machine (PGM™)上进行，使用片段文库(霰弹枪)。使用GS Reference Mapper程序对读取数据进行了比对。结果。所研究菌株的确定序列型- ST-152, sonnei链球菌最常见的基因型之一。结果表明，菌株S. sonnei 53G和S. sonnei Ss046的染色体序列与质粒А， В， С * E序列具有高度的相似性。鉴定出与大肠杆菌026:H11毒力质粒p026-Vir序列高度相似的序列
{"title":"EFFECT OF EXOPOLISACCHARIDES OF LACTIC ACID BACTERIA ON THE SYNTHESIS OF PROINFLAMMATORY CYTOKINES BY MACROPHAGIC MICE IN PHAGOCYTOSIS OF STAPHYLOCOCCUS AUREUS","authors":"G. T. Uryadova, E. Gorelnikova, N. Fokina, A. S. Dolmashkina, L. Karpunina","doi":"10.36233/0372-9311-2018-1-67-71","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-67-71","url":null,"abstract":"Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals. Aim. Study of molecular-genetic properties of Shigella sonnei- 2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a genetic analyzer Ion Torrent Personal Genome Machine (PGM™) using fragment libraries (shot-gun). Aligning reads have been carried out with the program GS Reference Mapper. Results. Defined sequence — type of the studied strain — ST-152, one of the most common genotypes for S. sonnei. Demonstrated the high degree of similarity obtained contig to the sequences of the chromosome and plasmids А, В, С и E strains S. sonnei 53G and S. sonnei Ss046. Identified contigs with a high percentage similarity to the se quence of virulence plasmid p026-Vir of E. coli 026:H11","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"29 1","pages":"67-71"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81170411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-28DOI: 10.36233/0372-9311-2018-1-3-9
О. В. Бухарин, Е. В. Иванова, Н. Б. Перунова, Игорь Анатольевич Никифоров
Aim. Aim of the research is the identification of functional groups of human gut bifidoflora based on analysis of the spectrum of metabolites features, proteome, bioprofile, immunoregulatory properties and the ability to differentiate «self/non-self» among the associative microbiota. Materials and methods. The materials are 260 strains ofbifidobacteria isolated from 122 intestinal microsymbiocenoses. Experimental studies were carried out using bacteriological, chromatographic and immunological methods. Statistical processing of material is carried out by means of the package Statistica 10.0 using of k-cluster analysis and discriminant method. Results. As a result ofthe work, 3 clusters containing strains of various types of bifidobacteria were identified. The first cluster was represented by B. bifidum and was characterized by the antipeptide activity of the strains with respect to FNO-a and INF-y, IL-10. In the second cluster of the B. longum culture predominated, where the parameters of the backbone factor of microsymbiocenosis, the ability to microbial recognition, antagonistic activity and production of acetic acid were significant. In the third cluster the species composition of bifidobacteria was diverse and products of butyric, caproic acids and their isoforms were the informative tests. Conclusion. The key function of bifido-flora in the regulation of the homeostasis of the intestinal biotope is realized by the formation of functional clusters, among which the first group participates in the formation of the cytokine balance, the second group is responsible for the discrimination of associative microbiota and direct protection of the biotope from pathogens, and the third is necessary to maintain the barrier metabolic function of enterocytes in the human large intestine.
{"title":"ФУНКЦИОНАЛЬНЫЕ ГРУППЫ БИФИДОФЛОРЫ КИШЕЧНОЙ МИКРОБИОТЫ В АССОЦИАТИВНОМ СИМБИОЗЕ ЧЕЛОВЕКА","authors":"О. В. Бухарин, Е. В. Иванова, Н. Б. Перунова, Игорь Анатольевич Никифоров","doi":"10.36233/0372-9311-2018-1-3-9","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-3-9","url":null,"abstract":"Aim. Aim of the research is the identification of functional groups of human gut bifidoflora based on analysis of the spectrum of metabolites features, proteome, bioprofile, immunoregulatory properties and the ability to differentiate «self/non-self» among the associative microbiota. Materials and methods. The materials are 260 strains ofbifidobacteria isolated from 122 intestinal microsymbiocenoses. Experimental studies were carried out using bacteriological, chromatographic and immunological methods. Statistical processing of material is carried out by means of the package Statistica 10.0 using of k-cluster analysis and discriminant method. Results. As a result ofthe work, 3 clusters containing strains of various types of bifidobacteria were identified. The first cluster was represented by B. bifidum and was characterized by the antipeptide activity of the strains with respect to FNO-a and INF-y, IL-10. In the second cluster of the B. longum culture predominated, where the parameters of the backbone factor of microsymbiocenosis, the ability to microbial recognition, antagonistic activity and production of acetic acid were significant. In the third cluster the species composition of bifidobacteria was diverse and products of butyric, caproic acids and their isoforms were the informative tests. Conclusion. The key function of bifido-flora in the regulation of the homeostasis of the intestinal biotope is realized by the formation of functional clusters, among which the first group participates in the formation of the cytokine balance, the second group is responsible for the discrimination of associative microbiota and direct protection of the biotope from pathogens, and the third is necessary to maintain the barrier metabolic function of enterocytes in the human large intestine.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90966245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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