Analytica Chimica Acta最新文献

{"title":"A ZnO-nanorod/PEDOT:PSS nanocomposite functionalized bridge-like membrane type nanomechanical sensing device for ultrasensitive blood lead detection","authors":"Yi-Kuang Yen ,&nbsp;Chia-Ming Yang ,&nbsp;Chen-Ting Kao ,&nbsp;Tzung-Hai Yen ,&nbsp;Ragurethinam Shanmugam ,&nbsp;Yan-Lin Chen ,&nbsp;Hwai-En Lin","doi":"10.1016/j.aca.2024.343317","DOIUrl":"10.1016/j.aca.2024.343317","url":null,"abstract":"<div><div>Lead (Pb) ion detection poses a critical problem, particularly in environmental monitoring, industrial operations, and public health, especially for young children and expecting women. Determining lead levels in blood early on is essential to minimizing the long-term consequences of lead exposure. Several sophisticated detection instruments, such as mass spectrometers which perform with high sensitivity, specificity and accuracy, but require a lab-based setting, multi-step sample preparation, expensive payment and professional operation. It is evident that a highly sensitive, portable, low-cost, quick sample-to-result, blood lead detection device that can be tested at the point-of-care is necessary. Consequently, we developed a unique ZnO/PEDOT:PSS nanocomposite layer integrated with a CMOS MEMS-based bridge-like membrane-type (BM) nanomechanical sensor for detecting lead levels in blood. PEDOT:PSS was combined with ZnO nanorods to increase lead ion binding. The sensor responds seven times better to lead ions using nanorods in the detecting layer. A linear resistance change rate response was found from 0.005 to 10 ppm, with the limit of detection (LOD) of 0.12 ppb. Similarly, our BM nanomechanical sensor can correctly assess Pb²⁺ in human serum with recovery rates of 86.25–150 %. Measurements of human blood samples from patients with varying lead ion concentrations validated by the standard AAS show a good linear connection with the BM nanomechanical sensors' concentration, with a regression coefficient of 0.92. This describes the first micromachined nanoachanical sensing system for detection of Pb²⁺ in only 5 μL of human serum sample. The device achieves a time-to-result of less than 10 min. The system is designed to be very sensitive and offers affordable, disposable sensing chips together with a portable signal acquisition platform.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343317"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of monoclonal antibody charge variants under near-native separation conditions using nanoflow sheath liquid capillary electrophoresis-mass spectrometry","authors":"Annika A.M. van der Zon ,&nbsp;Alisa Höchsmann ,&nbsp;Tijmen S. Bos ,&nbsp;Christian Neusüß ,&nbsp;Govert W. Somsen ,&nbsp;Kevin Jooß ,&nbsp;Rob Haselberg ,&nbsp;Andrea F.G. Gargano","doi":"10.1016/j.aca.2024.343287","DOIUrl":"10.1016/j.aca.2024.343287","url":null,"abstract":"<div><h3>Background</h3><div>Monoclonal antibodies (mAbs) undergo multiple post-translational modifications (PTMs) during production and storage, resulting for instance in charge and oxidized variants. PTMs need to be assessed as critical quality attributes to assure protein quality and safety. Capillary zone electrophoresis (CZE) enables efficient charge-based separation. The CZE method developed by He et al. (2011) is currently applied routinely in the pharmaceutical industry for profiling charge heterogeneity of mAbs. However, as the method relies on a non-volatile background electrolyte (BGE), it cannot be directly hyphenated with mass spectrometry (MS), hampering the identification of separated charge variants.</div></div><div><h3>Results</h3><div>This study presents a CZE-UV/MS method using a neutral static capillary coating of hydroxypropyl methylcellulose combined with a volatile BGE at pH 5.0 to allow for MS-compatible mAb charge variant separations. The effect of several parameters, including pH and concentration of the BGE, applied voltage, and injected mAb concentrations on separation performance was investigated using a panel of commercially available mAbs. The optimized method was evaluated with IgG₁ and IgG₄ mAbs of varying pI (7.4–9.2) and degrees of heterogeneity. Basic and acidic variants were separated from the parent mAb using a BGE of 50 mM acetic acid adjusted to pH 5.0 with ammonium hydroxide. The relative abundances of charge variants determined with the new method showed a good correlation with the corresponding relative levels obtained with the method of He et al. CZE-MS coupling was accomplished using the nanoCEasy, a low-flow sheath liquid interface, which enabled the identification and quantitation of basic, acidic, and incomplete pyroglutamate variants, and glycoforms of the tested mAbs.</div></div><div><h3>Significance</h3><div>This manuscript describes a new CZE-MS method that permits heterogeneity assessment of mAbs under MS-compatible conditions, providing charge variant separation.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343287"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal–to–particle charge transfer invoked photoelectrochemistry on ferroelectric SrTiO3 for split–mode and high–throughput aptasensing","authors":"Qi Zhang,&nbsp;Mengmeng Gu,&nbsp;Lan Zhang,&nbsp;Xiuming Wu,&nbsp;Guang-Li Wang","doi":"10.1016/j.aca.2024.343318","DOIUrl":"10.1016/j.aca.2024.343318","url":null,"abstract":"<div><div>Split–mode aptasensing is highly desirable in photoelectrochemistry because of its distinctive advantages of high-throughput, avoided damage to biomolecules, and increased sensitivity and selectivity. However, the currently available photoelectrochemical (PEC) strategy conducible to split–mode aptasensing is still limited to the bioreaction mediated generation of photoactive species, in which a low photocurrent was usually attained, rending this strategy impotent for attaining high sensitivity. As a result, to explore new strategies that are amendable to highly efficient, split-mode PEC aptasensing are still challenging but demanding. Herein, ferrocyanide mediated metal–to–particle charge transition (MPCT) on ferroelectric strontium titanate (SrTiO₃) was explored as an innovative signal transduction strategy and was validated for high-performance aptasensing. By taking the 17β–Estradiol (E2) as a model analyte, the recognition between the target (E2) and its aptamer anchored on the Fe₃O₄@Au (named as Fe₃O₄@Au/Apt) destroyed the beforehand formed assembly between the Fe₃O₄@Au/Apt and the ssDNA labeled liposome encapsulated with [Fe(CN)₆]^4- (named as DLL–FeCN), which resulted in the release of the beforehand encapsulated [Fe(CN)₆]^4- into solution. The released [Fe(CN)₆]^4- then coordinated onto the surface of SrTiO₃ nanoparticles consisted photoelectrode, forming the MPCT process from metal ion (iron (II) in [Fe(CN)₆]^4-) to the conduction band (CB) of SrTiO₃ for anodic photocurrent signal output. The detection achieved linear range of 1.0 pM–100 nM, with a detection limit of 0.3 pM for E2. Benefiting from the cooperative effects of the MPCT process and the ferroelectric polarization in bulk SrTiO₃ for achieving highly efficient photocurrent generation capability, the developed split–type detection had the advantage of high sensitivity/selectivity and high throughput. This work not only opens up the MPCT process for innovative PEC sensing strategy but also blazes a new road for high performance PEC aptasensing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343318"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of deliquescent chloride salt by laser-induced breakdown spectroscopy with controlled uniform precipitation","authors":"Lizhu Kang ,&nbsp;Ji Chen ,&nbsp;Zhijie Huang ,&nbsp;Zhanjian Lin ,&nbsp;Rui Zhang ,&nbsp;Bing Lu ,&nbsp;Xiangyou Li","doi":"10.1016/j.aca.2024.343315","DOIUrl":"10.1016/j.aca.2024.343315","url":null,"abstract":"<div><h3>Background</h3><div>The electrolytic efficiency in magnesium (Mg) production by molten salt electrolysis is mainly affected by the chloride content, which is determined by the metal content in the cooled molten salt. Laser-induced breakdown spectroscopy (LIBS) is a potential element detection method in cooled molten salt element detection. However, the cooled molten chloride salt is easily deliquescent, which greatly affects the LIBS detection results.</div></div><div><h3>Results</h3><div>To solve the problem, a liquid-phase precipitation method based on the addition of a dispersant named as controlled uniform precipitation (CUP) method was proposed to pretreat the samples. Compared with the conventional powder compacting method and precipitation method, the CUP obtained the highest spectral stability and detection accuracy. The average relative standard deviation (ARSD) of Mg, Ca, and Na decreased from the 51.80%, 77.04%, and 44.01% to 6.77%, 6.27%, and 29.97%, the determination coefficient of the calibration curve (R²) increased from 0.044, 0.084, and -0.109 to 0.974, 0.954, and 0.802, and the average relative error (ARE) decreased from 58.49%, 52.46%, and 99.78% to 8.58%, 11.28%, and 29.38%, respectively, compared with the powder compacting method. Further investigation showed that the CUP method suppressed the sample deliquescence and the coffee ring effect, leading to the performance improvement of LIBS quantitative detection.</div></div><div><h3>Significance</h3><div>The CUP provided an effective method of detecting metal elements for deliquescent samples and showed applied potential for other precipitable elements detection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343315"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oriental covalent immobilization of N-glycan binding protein via N-terminal selective modification","authors":"Wenhui Wang ,&nbsp;Liang Zhang ,&nbsp;Yuanyuan Liu ,&nbsp;Xiang Liu ,&nbsp;Xin Liu","doi":"10.1016/j.aca.2024.343311","DOIUrl":"10.1016/j.aca.2024.343311","url":null,"abstract":"<div><div>Lectin affinity chromatography is one of powerful tools for the study of protein glycosylation. Different lectin proteins can recognize different structures of monosaccharides or oligosaccharide units, allowing for the selective separation of glycopeptides or glycoproteins containing different polysaccharide structures. However, the N-glycans were only partially captured by most of common lectins, reducing the coverage rate of identifying N-glycoconjugates. Recently, it has been reported that the engineering variant of glycan binding protein Fbs1 has a high affinity for innermost Man₃GlcNAc₂ structure and is able to bind diverse types of N-glycans, which can be suitable for the analysis of protein N-glycosylation. However, efficient immobilization of protein to separation matrix is particularly challenging as it requires the functionality and integrity of the protein to be preserved. Herein, we describe a simple and robust strategy for oriental covalent immobilization of proteins on magnetic nanoparticles by N-terminal selective labeling techniques. We inserted the enterokinase cleavage site to produce the specific N- terminal glycine of protein. Under physiological conditions, the protein was immobilized on the surface of the MNPs by this glycine tag, and the enrichment process could be completed within 30 min. A whole enrichment and purification of glycan and glycopeptides were completed and analyzed by MALDI TOF-MS. The functional materials achieved stable enrichment of glycan structure in different enzyme digestion systems or complex samples, showing excellent anti-interference and applicability.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1330 ","pages":"Article 343311"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards the non-invasive determination of estradiol levels: Development and validation of an LC-MS/MS assay for quantification of salivary estradiol at sub-pg/mL level","authors":"David Fabregat-Safont ,&nbsp;Élida Alechaga ,&nbsp;Noemí Haro ,&nbsp;Àlex Gomez-Gomez ,&nbsp;Eric R. Velasco ,&nbsp;Jaime F. Nabás ,&nbsp;Raül Andero ,&nbsp;Oscar J. Pozo","doi":"10.1016/j.aca.2024.343313","DOIUrl":"10.1016/j.aca.2024.343313","url":null,"abstract":"<div><h3>Background</h3><div>Estradiol (E2) is a female sex hormone involved in several biological processes. Although E2 levels are commonly measured in blood samples, the use of non-invasive techniques (e.g. determination of salivary E2) would allow for the collection of repeated samples and the inclusion of a greater number of participants. Immunoassay-based techniques to measure salivary E2 failed to accurately mirror the variations observed in the plasmatic concentrations of E2 during the menstrual cycle probably due to the high sensitivity required (in the sub-pg/mL range). Therefore, sensitive and rugged analytical methods for the determination of salivary E2 are required. For this, we developed and validated an analytical methodology for the accurate determination of salivary E2.</div></div><div><h3>Results</h3><div>The method is based on chemical derivatization with 1,2-dimethyl-1H-imidazole-5-sulphonyl chloride and liquid chromatography-tandem mass spectrometry analysis by summing highly-specific SRM transitions. This strategy allowed for increasing the sensitivity of the method. The validation of the method showed an accurate and precise quantification of E2 in 1 mL of saliva even at 250 fg/mL (97 % accuracy and 15 % RSD intra-day, and 104 % accuracy and 18 % RSD inter-day). In order to evaluate its efficacy, we analysed saliva samples from 5 healthy female volunteers collected during a whole menstrual cycle. Our analyses showed that the variations in the concentration of E2 in the measured samples mirrored those expected during a complete menstrual cycle. Additionally, we validated the suitability of our method for determining salivary E2 levels during pregnancy.</div></div><div><h3>Significance</h3><div>To the best of our knowledge, this is the first method that allows to precisely and accurately measuring E2 in saliva samples along the whole menstrual cycle of healthy females. It is also suitable for the determination of estradiol during pregnancy. Its high sensitivity makes this strategy ideal for the evaluation of the role of hormone production in women's health.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343313"},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an accurate synchronous transport signal hand-held sensing platform for fluorescence-based berberine on-site detection","authors":"Liucheng Guo ,&nbsp;Liyue Du ,&nbsp;Yan Zhang ,&nbsp;Jie Gao ,&nbsp;Fengling Cui","doi":"10.1016/j.aca.2024.343304","DOIUrl":"10.1016/j.aca.2024.343304","url":null,"abstract":"<div><h3>Background</h3><div>Berberine is widely used in clinical treatment because of its wide antibacterial spectrum and low toxic side effects. However, its abuse could lead to bacterial resistance and several other adverse effects. In addition, measuring the content of berberine in environmental water samples helps to monitor its accumulation and metabolism in ecosystems. Traditional detection methods usually need to be carried out in the laboratory, involving complex processing procedures, which are not only time-consuming, but also unfavorable for rapid response and decision-making. Therefore, it is necessary to develop portable instruments to provide reasonable guidance on the addition and intake of berberine to reduce the harm caused by its abuse.</div></div><div><h3>Results</h3><div>In this work, an accurate synchronous transport signal hand-held sensing platform (STSHSP) with a low-cost, easy-to-manufacture, independent use was developed by using photoelectric conversion technology, Bluetooth technology, remote synchronous signal technology, electrical technology, and 3D printing technology. To verify the performance of STSHSP, a 5-oxo-2,3-dihydro-5<em>H</em>-thiazolo [3,2-a] pyridine-3,7-dicarboxylic acid (TPDCA) with ultra-high quantum yield was designed and synthesized as a probe. TPDCA exhibited bright blue fluorescence under the ultraviolet light of 365 nm which could be quenched by berberine through the inner filter effect. In the range of 0.1–80 μg/mL, the voltage displayed by the prepared STSHSP has a good linearity with the berberine concentration (R² = 0.9997) with a detection limit of 28.32 ng/mL. The portable sensor demonstrated good stability, accuracy, and reliability in detecting actual river water, urine, traditional Chinese medicine, and its preparation samples.</div></div><div><h3>Significance</h3><div>The sensor with its compact structure, portability, and simple operation was suitable for in-situ detection with accurate, reliable, and feasible results, which is beneficial for improving drug quality and ensuring human health. Fortunately, the device could transmit the information to the control center and/or a third-party supervision institution in real-time, which could effectively eliminate the trust crisis. The sensor has broad application prospects in the field of environmental water quality detection and drug safety.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343304"},"PeriodicalIF":5.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensity- and time-based strategies for micro/nano-sizing via single-particle ICP-mass spectrometry: A comparative assessment using Au and SiO2 as model particles","authors":"Antonio Bazo,&nbsp;Eduardo Bolea-Fernandez,&nbsp;Ana Rua-Ibarz,&nbsp;Maite Aramendía,&nbsp;Martín Resano","doi":"10.1016/j.aca.2024.343305","DOIUrl":"10.1016/j.aca.2024.343305","url":null,"abstract":"<div><h3>Background</h3><div>Single-particle ICP-mass spectrometry (SP-ICP-MS) is a powerful method for micro/nano-particle (MNP) sizing. Despite the outstanding evolution of the technique in the last decade, most studies still rely on traditional approaches based on (1) the use of integrated intensity as the analytical signal and (2) the calculation of the transport efficiency (TE). However, the increasing availability of MNP standards and advancements in hardware and software have unveiled new venues for MNP sizing, including TE-independent and time-based approaches. This work systematically examines these different methodologies to identify and summarize their strengths and weaknesses, thus helping to determine their preferred application areas.</div></div><div><h3>Results</h3><div>Different SP-ICP-MS methods for MNP sizing were assessed using AuNPs (20–70 nm) and SiO₂MNPs (100–1000 nm). Among TE-dependent approaches, the particle frequency method was characterized by larger uncertainties than the particle size method. The results of the latter were dependent on the appropriate selection of the reference MNP, making the use of multiple reference MNPs recommended. TE-independent methods were based on external (linear and polynomial) calibrations and a relative approach. These methods exhibited the lowest uncertainties of all the strategies evaluated. External calibrations benefited from simpler calculations, but their application could be hindered by a lack of reference MNPs within the desired size range or by the need for interpolations outside the calibration range. Finally, transit time signals are directly proportional to the MNP size rather than its mass. The time-based method demonstrated adequate performance for sizing AuNPs but failed when sizing the largest SiO₂MNPs (1000 nm).</div></div><div><h3>Significance and novelty</h3><div>This work provides further insights into the application of different SP-ICP-MS methodologies for MNP sizing. Both TE-independent approaches and the monitoring of the transit time as the analytical signal are underused strategies; in this context, a Python script was developed for accurate transit time measurement. After 20 years of development, a quantitative comparison of the different methodologies, including the most novel approaches, is deemed necessary for further growth on solid theoretical ground.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343305"},"PeriodicalIF":5.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of complexing membranes for platinum analysis in water at part per trillion levels by X-ray fluorescence","authors":"Georgia P. Vlamaki,&nbsp;Nikolaos G. Kallithrakas-Kontos","doi":"10.1016/j.aca.2024.343306","DOIUrl":"10.1016/j.aca.2024.343306","url":null,"abstract":"<div><h3>Background</h3><div>Platinum is a critical metal, especially in medicine and catalysis. Its concentration in aquatic ecosystems may gradually affect aquatic animals due to its ability to bioaccumulate. Additionally, the recycling of platinum has become crucial, so new extraction methods are needed. In recent years, there has been increasing interest in environmental studies based on X-Ray Fluorescence, due to the requirements of green chemistry. Complexing membranes have been used for platinum analysis in combination with X-ray Fluorescence, although with a relatively high detection limit.</div></div><div><h3>Results</h3><div>Several optimizations were introduced to achieve a significantly improved detection limit and repeatability. Self-supported membranes were used for the first time and proved to be highly effective in binding platinum from aqueous solutions. An extensive search was conducted to identify an appropriate chemical element to serve as an internal standard; osmium met many of the necessary criteria and was successfully used as an internal standard, leading to a remarkable improvement in the method's precision. The effect of various elements present in natural waters was examined, and no interferences were observed. The method gave equally reliable results in all types of water, including seawater. Through substantial optimization procedures, a minimum detection limit up of 37 ng L⁻¹ was achieved with a 1000 s irradiation time. The potential aging of the membrane was investigated, showing that the membrane does not deteriorate for at least one month when stored at refrigeration temperature.</div></div><div><h3>Significance</h3><div>The newly introduced self-supported complexing membranes improved the platinum detection limit by more than 100-fold, enabling, for the first time, platinum analysis at low ng L⁻¹ concentration levels using X-ray Fluorescence. This method can be used to various types of natural waters, including seawater with near-complete recovery from the aquatic matrix. This research may also contribute to improving the minimum detection limits of the method for other trace elements besides platinum.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343306"},"PeriodicalIF":5.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel dual-ligand copper-based nanoflower for the colorimetric and fluorescence detection of 2,4-dichlorophenol, epinephrine and hydrogen peroxide","authors":"Jia-Xin Li ,&nbsp;Tong-Qing Chai ,&nbsp;Guo-Ying Chen ,&nbsp;Mao-Ling Luo ,&nbsp;Jian-Bo Wan ,&nbsp;Feng-Qing Yang","doi":"10.1016/j.aca.2024.343298","DOIUrl":"10.1016/j.aca.2024.343298","url":null,"abstract":"<div><h3>Background</h3><div>Nanozymes have the advantages of cost effective, simple synthesis, high durability and stability, and have been widely used in various fields. However, only a few nanomaterials with multiple enzyme-like activity have been reported, and most of the currently developed nanozymes are usually used in colorimetric or fluorescence analysis depending on a single colorimetric or fluorescence signal output. In this study, a copper-based dual-ligand biomimetic nanoflower (Cu-MN) was constructed, which demonstrated potential multiple enzyme-like activity, and was applied to the multi-mode detection of 2,4-dichlorophenol (2,4-DP), epinephrine (EP), and H₂O₂.</div></div><div><h3>Results</h3><div>The laccase-like activity of Cu-MN can catalyze the conversion of 2,4-DP and EP, resulting in the formation of red and yellow-brown oxidation products with distinct UV absorption peaks at 510 nm and 485 nm, respectively. Furthermore, the fluorescence emission peak at 426 nm of Cu-MN can be dynamic quenched during substrate oxidation due to the fluorescence internal filtration effect (IFE). Therefore, a dual-mode analysis method was constructed to detect 2,4-DP and EP by fluorescence and ultraviolet colorimetry, which was successfully applied in natural lake water and rabbit plasma analysis, respectively. Furthermore, a colorimetric sensing strategy based on the peroxidase-like activity of Cu-MN was developed and successfully applied to the monitoring of H₂O₂ in hydrogen peroxide disinfectant. Additionally, the visualization analysis method was also established by RGB reading of the smartphone.</div></div><div><h3>Significance and novelty</h3><div>In brief, inspired by the fluorescence characteristics of 2-aminoterephthallc acid and the imidazole group of 2-methylimidazole, a novel copper-based dual-ligand biomimetic nanoflower (Cu-MN) was prepared and used to establish multi-mode method for the detection of 2,4-DP, EP, and H₂O₂, which opens up new avenues for its applications in bioanalysis and environmental monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1330 ","pages":"Article 343298"},"PeriodicalIF":5.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}