Analytica Chimica Acta最新文献

{"title":"Advancing elemental analysis by collision/reaction cell technology and micro-droplet calibration for bioimaging applications by LA-ICP-TOFMS","authors":"Sarah Theiner ,&nbsp;Elisabeth Foels ,&nbsp;Gunda Koellensperger","doi":"10.1016/j.aca.2024.343345","DOIUrl":"10.1016/j.aca.2024.343345","url":null,"abstract":"<div><h3>Background</h3><div>Bioimaging applications by laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) require comprehensive elemental analysis spanning the entire mass range. Within biological systems, endogenous elements are critical for maintaining metal homeostasis in cells, a fundamental aspect in disease progression when disrupted. Additionally, elements from the higher mass range may originate from various sources such as metal-tags for immuno-mass spectrometry, nanoparticles, or metal-based anticancer drugs. This study assesses the efficacy of collision/reacton cell (CCT) mode for simultaneously analysing elements across the complete mass spectrum. Furthermore, we demonstrate the accuracy of the LA-ICP-TOFMS methodology through the analysis of serum reference material.</div></div><div><h3>Results</h3><div>Our findings demonstrate that the CCT mode outperforms the standard/no gas mode for LA-ICP-TOFMS measurements, particularly in quantifying endogenous elements susceptible to interferences. Through the analysis of picolitre-volume micro-droplet standards and serum reference material (deposited as micro-droplets), we observed accurate quantification of elements such as iron and selenium, with isotope ratios closely resembling natural compositions. As key advantage, the utilization of the CCT mode eliminated the need for labor-intensive post-data processing, streamlining analytical procedures. Additionally, the CCT mode also provided enhanced sensitivity (factor of 1.5–2) for elements in the higher mass range compared to the standard mode, without compromising the sensitivity for endogenous elements.</div></div><div><h3>Significance</h3><div>We successfully integrated Seronorm serum reference material-containing micro-droplets into LA-ICPMS bioimaging workflows for quality control, enabling validated measurements of key biological elements. The use of the CCT mode is highly recommended for bioimaging applications that address the analysis of elements across the entire mass range.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343345"},"PeriodicalIF":5.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142450128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selectivity in Nontargeted Qualitative Analysis","authors":"A.L. Pomerantsev,&nbsp;O.Ye. Rodionova","doi":"10.1016/j.aca.2024.343352","DOIUrl":"10.1016/j.aca.2024.343352","url":null,"abstract":"<div><h3>Background</h3><div>The concept of selectivity is used extensively in analytical chemistry in both quantitative and qualitative analyses. While in the first case, the definition and use of selectivity have been somehow regulated, in the second case it remains a vague concept.</div></div><div><h3>Results</h3><div>In this paper we propose a new definition of selectivity in qualitative analysis as well as the way to estimate it numerically.</div></div><div><h3>Significance</h3><div>It is shown how selectivity can be used practically to compare analytical objects, measurement techniques, and data modeling methods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343352"},"PeriodicalIF":5.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142450126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of a novel β-cyclodextrin chiral stationary phase and its application to the evaluation of the enantioselective bioaccumulation and elimination behavior of tebuconazole in Rana nigromaculata tadpoles","authors":"Linbin Zheng ,&nbsp;Meng Li ,&nbsp;Zhouyu Jiang ,&nbsp;Junyan Fan ,&nbsp;Zengbo Fang ,&nbsp;Jihuai Zheng ,&nbsp;Yan Cui","doi":"10.1016/j.aca.2024.343344","DOIUrl":"10.1016/j.aca.2024.343344","url":null,"abstract":"<div><h3>Background</h3><div>The increased production and use of chiral pesticides will enhance their exposure in the environment. Chiral pesticides typically exhibit varied biological effects among these enantiomers. Therefore, it is very essential to develop and validate chiral analytical methods to investigate their potential ecological risks from a stereoselective perspective. Current separation of pesticides enantiomers relies extensively on chiral stationary phases (CSPs), while the development of β-Cyclodextrin derivatives CSPs become the research focus due to their great modifiability and excellent chiral recognition capabilities.</div></div><div><h3>Results</h3><div>A novel chiral stationary phase, 3,5-dichlorophenylaminomethyl-6-phenylenediamine-β-cyclodextrin chemically bonded silica gel (MPDCDA), was successfully prepared. Based on that, a stereoselective HPLC-MS/MS method was developed and validated for the determination of tebuconazole enantiomers in <em>Rana nigromaculata</em> tadpoles. After extraction by QuEChERS, the tebuconazole enantiomers were completely separated with the resolutions of 1.63 using the mobile phase of methanol-water (70/30, v/v). Good linearity (r &gt; 0.9990) for both enantiomers over a concentration range of 0.20–500.0 ng/mL was obtained with the accuracy ranged from 6.7 % to 9.3 % and the intra-day and inter-day precisions below 6.2 % at three quality control levels. The proposed method was successfully applied in evaluating the enantioselective bioaccumulation and elimination profiles of tebuconazole in tadpoles. At the tested conditions, there was no significantly enantioselective difference in the bioaccumulation process for <em>S</em>- tebuconazole and <em>R</em>-tebuconazole. However, the elimination process of tebuconazole enantiomers was enantioselective with <em>R</em>-tebuconazole preferentially degraded.</div></div><div><h3>Significance</h3><div>This work provided an accurate risk assessment of chiral pesticides to non-target aquatic organisms from a stereoselective perspective. These findings would deepen our understanding of the potential ecological risks of chiral pesticides on aquatic organisms and provide scientific support for the protection of aquatic organisms and their ecological environments, as well as sustainable development.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343344"},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142450066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel near-infrared fluorescent probe for butyrylcholinesterase: Research for screening of natural anti-AD inhibitors","authors":"Xiaoyi Yin ,&nbsp;Gaoning Zhang ,&nbsp;Guangxu Song ,&nbsp;Xiaoru Li ,&nbsp;Xinming Liu ,&nbsp;Lufan Wang ,&nbsp;Hai Zhang ,&nbsp;Zhixin Tang","doi":"10.1016/j.aca.2024.343348","DOIUrl":"10.1016/j.aca.2024.343348","url":null,"abstract":"<div><div>Elevated levels of butyrylcholinesterase (BuChE) have the potential to be predictive in the timely detection and diagnosis of Alzheimer's disease (AD). By inhibiting of BuChE activity can raise acetylcholine levels and intervene AD processes. Therefore, BuChE as an important factor in treatment AD has been given more and more attention in clinical studies. Given the facts above, in this study, for precise monitoring of BuChE level changes and screening for possible butyrylcholinesterase inhibitor (BuChEI) for AD diagnosis and therapy, a near-infrared (NIR) fluorescence probe (<strong>NFP-BuChE</strong>) was created. The probe exhibits excellent sensitivity and selectivity for BuChE. <strong>NFP-BuChE</strong> has been successfully applied to the detection of endogenous BuChE levels in live cells, and we successfully constructed a screening system for BuChEI on cells and a novel natural efficient BuChEI (matrine) was discovered and identified, which significantly reduced BuChE activity and thus alleviated AD symptoms. Most importantly, for the first time, we measured the changes of BuChE levels in zebrafish (0–4 days) after fertilization, various organs of zebrafish, and AD zebrafish modeled by different concentrations of AlCl₃ by <strong>NFP-BuChE</strong>, and at the same time, we also validated the inhibitory effect of matrine on BuChE by <strong>NFP-BuChE</strong> in zebrafish. In addition, <strong>NFP-BuChE</strong> has also been successfully used to measure the changes of BuChE levels in the brains of AD mice. These findings imply that <strong>NFP-BuChE</strong> is a potentially useful molecular tool for screening possible natural BuChEI quickly and for monitoring changes in BuChE activity, and it is expected that more value will be explored in mice. In addition, matrine and its derivatives are promising options for future Alzheimer's disease treatments.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343348"},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radical-directed dissociation mass spectrometry for differentiation and relative quantitation of isomeric ether-linked phosphatidylcholines","authors":"Xiaohui Zhang ,&nbsp;Qinhua Chen ,&nbsp;Lun Wu ,&nbsp;Wenpeng Zhang ,&nbsp;Xue Zhao","doi":"10.1016/j.aca.2024.343337","DOIUrl":"10.1016/j.aca.2024.343337","url":null,"abstract":"<div><h3>Background</h3><div>Ether-linked phosphatidylcholines (PCs) include both plasmanyl and plasmenyl PCs, which contain an ether or a vinyl ether bond at the <em>sn</em>-1 linkage position, respectively. Profiling and quantifying ether PCs with accurate structural information is challenging because of the common presence of isomeric and isobaric species in a lipidome.</div></div><div><h3>Results</h3><div>In the present study, radical directed dissociation (RDD) from collision-induced dissociation (CID) of the bicarbonate anion adduct of ether PCs has been investigated to differentiate and relatively quantify ether PCs. Alkyl- and alkenyl- PCs give diagnostic characteristic fragment patterns that enable their confident identification and isomer differentiation. Additionally, the <em>sn</em>-position specific product ions have proven effective for relative quantitation among isomers in ether PCs and their isobaric PC species. Using this methodology, we successfully identified a total of 30 PC-O species, 21 PC-P species at the chain composition level, and 22 species of isobaric PC at the <em>sn</em>-position level in the human plasma lipid extract. The quantitative analysis revealed that ether PCs with a 20:4 fatty acyl chain are relatively more abundant in human plasma. Finally, the profile of ether PCs in type 2 diabetic (T2D) groups compared to normal control groups revealed a significant decrease in PC-O 18:1/20:5. We also found it is the PC species containing a 17-carbon fatty acyl chain, rather than their isobaric ether PCs, that shows a decreasing trend in the T2D groups.</div></div><div><h3>Significance</h3><div>ether-linked PCs are firstly investigated by RDD mass spectrometry.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343337"},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimising the acquisition conditions of high information quality low-field NMR signals based on a cutting-edge approach applying information theory and Taguchi's experimental designs – Virgin olive oil as an application example","authors":"Alejandra Arroyo-Cerezo ,&nbsp;Ana M. Jiménez-Carvelo ,&nbsp;Rosalía López-Ruíz ,&nbsp;María Tello-Liébana ,&nbsp;Luis Cuadros-Rodríguez","doi":"10.1016/j.aca.2024.343350","DOIUrl":"10.1016/j.aca.2024.343350","url":null,"abstract":"<div><h3>Background</h3><div>Developing a new spectrometric analytical method based on a fingerprinting approach requires optimisation of the experimental stage, particularly with novel instruments like benchtop low-field NMR spectrometers. To ensure high-quality LF-NMR spectra before developing the multivariate model, an experimental design to optimise instrument conditions is essential. However, difficult-to-control factors may be critical for optimisation. Taguchi methodology addresses these factors to obtain a system robust to variation. This study uses the Taguchi methodology to optimise instrument settings for acquiring high-quality ¹H and ¹³C LF-NMR signals in a short time from virgin olive oil (VOO).</div></div><div><h3>Results</h3><div>Two experimental trials (for ¹H and ¹³C signals, respectively) were carried out and analysed to find an optimal and robust combination of instrument settings against changes in two difficult-to-control factors: ambient temperature and small deviations of the NMR tube volume (700 ± 50 μL). The responses to be optimised, run time and spectral information quality, were analysed separately and jointly, as some factors showed opposite behaviour in the effect on the responses. Multiple response analysis based on suitable desirability functions yielded a combination of factors resulting in desirability values above 0.8 for ¹H LF-NMR signals and almost 1.0 for ¹³C LF-NMR signals.</div><div>In addition, a novel approach to assess the information quality of an analytical signal was proposed, addressing a major challenge in analytical chemistry. By applying information theory and calculating information entropy, this approach demonstrated its potential for selecting the highest quality (i.e. most informative) analytical signals.</div></div><div><h3>Significance</h3><div>The acquisition instrument conditions of LF-NMR were successfully optimised using Taguchi methodology to acquire highly informative ¹H and ¹³C spectra in a minimum run time. The importance lies in the future development of non-targeted analytical applications for VOO quality control. In addition, the innovative use of information entropy to <em>a priori</em> assess the signal quality represents a significant advance and proposes a solution to a long-standing challenge in analytical chemistry.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343350"},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-assisted extraction of polysaccharides from Micromelum minutum leaves using citric acid monohydrate-glycerol based deep eutectic solvents and evaluation of biological activities","authors":"Muhammad Hasnun Md Yusoff,&nbsp;Muhammad Hakimin Shafie","doi":"10.1016/j.aca.2024.343351","DOIUrl":"10.1016/j.aca.2024.343351","url":null,"abstract":"<div><h3>Background</h3><div>The extraction of polysaccharides using an acidic extraction media has been extensively reported, highlighting its effectiveness in yielding high-quality polysaccharides. A higher concentration of acidic solution could hydrolyze the structure of polysaccharide, while a low concentration reduces the extraction efficiency. Despite this challenges, deep eutectic solvents (DES) were introduced as an alternative extraction medium due to additional interactions such as inter and intra-molecular interactions, Van de Waals, hydrogen bond, and electrostatic interactions, which could improve the polysaccharide extraction efficiency and biological activities. Furthermore, the extraction conditions such as extraction medium and extraction parameters could affect the properties of polysaccharides as well as influence their structure-activity relationship for biological activities.</div></div><div><h3>Results</h3><div>The result showed that the microwave-assisted extraction of <em>Micromelum minutum</em> leaf polysaccharide (MMLP) using DES as an extraction media (MMLP-DES) gave a higher yield (improvement of 101.20 %) than citric acid monohydrate (CAM) (MMLP-CAM) and required a lower percentage of microwave power (19.83 % less) and time (0.78 min less). The properties of MMLPs significantly differ based on their pH, molecular weight, viscosity, degree of esterification and monosaccharide molar ratio which influenced the biological activities. Compared to MMLP-CAM, MMLP-DES had a more branched and less linear structure. The bioactivities study revealed that MMLP-DES exhibited higher antioxidant and anti-α-amylase activities (i.e., DPPH: 74.52 %, FRAP: 2.87 mM FeSO₄ and α-amylase inhibition: 86.23 %) compared to MMLP-CAM (i.e., DPPH: 49.33 %, FRAP: 1.49 mM FeSO₄, and α-amylase inhibition: 81.76 %). The mechanism and structure-activity relationship of MMLPs on bioactivities were also hypothesized.</div></div><div><h3>Significance</h3><div>Based on our previous study, the citric acid monohydrate-glycerol based DES as an extraction medium has enhanced the extraction yield of polysaccharides from <em>M. minutum</em>. This study highlights the DES combined with microwave-assisted extraction to improve the yield of MMLP and evaluate the biological activities compared to CAM as a classical solvent. In conclusion, the DES showed the advantages for extraction of polysaccharides with desired biological activities.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343351"},"PeriodicalIF":5.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive detection of Hg(II) on MoS2/NiS2 based on interfacial engineering to accelerate the Ni2+/Ni3+ cycle: Identification the role of atomic-level heterojunction-induced electron transfer in electroanalysis","authors":"Huan Xu,&nbsp;Hao-Ran Li,&nbsp;Jing-Yi Li,&nbsp;Jian-Jun Qu,&nbsp;Shan-Shan Li","doi":"10.1016/j.aca.2024.343339","DOIUrl":"10.1016/j.aca.2024.343339","url":null,"abstract":"<div><div>The valence change of transition metal ions in nanomaterials can highly enhance the electrochemical detection performance toward heavy metal ions (HMIs), and how to further promote the valence change calls enormous concerns in electroanalysis. In this work, an interfacial engineering that combing the MoS₂ and NiS₂ together to form the MoS₂/NiS₂ complex is proposed. The density functional theory (DFT) results reveals that the novel atomic-level heterojunction between MoS₂ and NiS₂ will build an internal electric field (IEF), which leads to an enhanced conductivity and valence change behavior of Ni atoms in MoS₂/NiS₂ complex, resulting in a superior detection performance. In detail, the formation of atomic-level heterojunctions in the MoS₂/NiS₂ complex accelerates electron transfer due to the valence changes associated with Ni²⁺/Ni³⁺ cycling. The active Mo⁴⁺ species on MoS₂ act as electron donors, facilitating the reduction of Ni³⁺ to Ni²⁺ on NiS₂, thereby promoting Ni²⁺/Ni³⁺ cycling. As anticipated, the MoS₂/NiS₂ complex exhibits exceptional detection performance for Hg(II), with a sensitivity of 459.13 μA μM⁻¹ cm⁻², surpassing even that of other composite materials. In general, these findings are expected to significantly advance the application of electron transfer acceleration in electroanalysis based on the construction of heterojunction.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343339"},"PeriodicalIF":5.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zwitterionic covalent organic nanosheets for selective analysis of domoic acid in marine environment","authors":"Jinhua Xu ,&nbsp;Zhenli Yu ,&nbsp;Wenmin Zhang ,&nbsp;Shiye Xie ,&nbsp;Baodi Liao ,&nbsp;Lan Zhang","doi":"10.1016/j.aca.2024.343343","DOIUrl":"10.1016/j.aca.2024.343343","url":null,"abstract":"<div><h3>Background</h3><div>Domoic acid (DA) is a neurotoxic compound causing amnesic shellfish poisoning, secreted by red algae and diatoms. As a glutamate analogue, DA accumulates in filter-feeding marine organisms, posing significant health risks to humans upon consumption. Detecting DA in marine environments remains challenging due to its low concentration and interference from complex matrices. Effective detection and removal require materials with high efficiency and selectivity, which traditional inorganic ionic materials lack due to their limited adsorption capacity and selectivity. Ionic covalent organic frameworks (iCOFs) expected to become highly efficient DA adsorbents due to tunable ionic sites.</div></div><div><h3>Results</h3><div>Thus, a zwitterionic covalent organic nanosheet (TGDB-iCONs) was synthesized to selectively capture DA. TGDB-iCONs was prepared by one-step Schiff-base reaction of the charged monomer triaminoguanidine hydrochloride. It uniformly distributed positively charged guanidinium and negatively charged chloride ions on the surface, forming zwitterionic binding sites. The self-peeling of TGDB-iCONs facilitated the exposure of active sites and improved the adsorption efficiency. Several binding forces were generated between TGDB-iCONs and DA, including complementary electrostatic hydrophilic interactions, which were verified by density functional theory (DFT) calculation. TGDB-iCONs exhibited ultra-fast adsorption kinetics (7 min) and relatively high adsorption capacity (66.48 mg/g) for DA. Furthermore, TGDB-iCONs exhibit strong salt resistance, which is attributed to the charge “shielding” effect of the zwitterionic ions present in TGDB-iCONs. TGDB-iCONs could highly selectively enrich DA and detect trace DA from marine environment including seawater, algae and marine organisms and the limit of detection as low as 0.3 ng/kg.</div></div><div><h3>Significance and novelty</h3><div>This comprehensive study not only sheds light on the vast potential of ionic covalent organic frameworks nanosheets (iCONs) in supporting early warning, control, and traceability of DA, but also lays a solid foundation for future research endeavors aimed at designing and harnessing the unique properties of iCONs.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1331 ","pages":"Article 343343"},"PeriodicalIF":5.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ electrochemically synthesized “artificial” Gly m TI antibody for soybean allergen quantification in complex foods","authors":"Catarina Dias ,&nbsp;Daniela Fernandes ,&nbsp;Joana Costa ,&nbsp;Caterina Villa ,&nbsp;Patrícia Moreira ,&nbsp;Ana T.S.C. Brandão ,&nbsp;Isabel Mafra ,&nbsp;A.Fernando Silva ,&nbsp;Carlos M. Pereira ,&nbsp;Renata Costa","doi":"10.1016/j.aca.2024.343340","DOIUrl":"10.1016/j.aca.2024.343340","url":null,"abstract":"<div><div>Biosensors, especially those designed for detecting food allergens like Gly m TI in soybean, play a crucial role in safeguarding individuals who suffer from adverse food allergies, extending to both individual well-being and broader public health considerations. Furthermore, their integration into food production and monitoring processes aids in compliance with regulatory standards, reducing the incidence of allergen-related recalls and protecting vulnerable populations. Technological advancements in biosensor development, such as increased portability, real-time monitoring capabilities, and user-friendly interfaces, have expanded their practical applications, making them indispensable in various settings, including manufacturing plants, food service establishments, and even at-home use by consumers.</div><div>For the first time, a biosensor targeting the Gly m TI allergen based on molecularly imprinted polymer (MIP) technology was developed to detect/quantify soybean in complex food matrices and effectively address the detection challenges of complex and processed foods. The Gly m TI-MIP underwent a thorough validation process using anti-Gly m TI IgG raised as a polyclonal response to the trypsin inhibitor. Gly m TI-MIP was successfully tested across a range of food matrices, including tree nuts (e.g., peanuts, walnuts, and hazelnuts) and legumes (e.g., lentils, beans, and lupine), presenting minimal cross-reactivity with lupine and walnut. The innovative approach provided a linear response in the 1 ag mL⁻¹ - 10 μg mL⁻¹ range, with a LOD&lt;1 ag mL⁻¹. Applying the Gly m TI-MIP sensor to complex model foods allowed to detect 0.1 mg kg⁻¹ (0.00001 %) of soybean protein isolate in biscuits, ham, and sausages before and after the respective thermal treatments. The innovative biosensor can significantly improve food safety protocols by addressing the complexities of tracing allergens in processed and unprocessed food products. By ensuring rigorous allergen control, these biosensors may support global food trade compliance with international safety standards, boost consumer confidence, and promote transparency in food labeling, ultimately contributing to a safer food supply chain.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343340"},"PeriodicalIF":5.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}