Analytica Chimica Acta最新文献_第4页

A multi-functional effervescent tablet for one-pot effervescent/derivatization/extraction: An integrated strategy for sulfonamide residue analysis 一种一锅泡腾/衍生/提取多功能泡腾片：磺胺残留综合分析策略

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-02-03 DOI: 10.1016/j.aca.2026.345186

Di Chen , Yixin Ma , Xirui Pei , Wenxuan Li , Chenqi Niu , Haobo Zheng

Background

Sulfonamides are extensively used antimicrobials, but residues in food raise health risks such as allergies, antimicrobial resistance, and carcinogenicity. Strict residue limits require sensitive and efficient monitoring methods. While LC-FLD is selective and practical, conventional workflows rely on multi-step extraction and derivatization, which are time-consuming. A streamlined strategy integrating extraction and derivatization into a single operation is needed for practical residue analysis in complex food matrices.

Results

A novel multi-functional effervescent tablet was developed to unify effervescence, derivatization, and extraction in one step. The tablet incorporates effervescent precursors (Na₂CO₃/NaH₂PO₄), fluorescamine (a derivatization reagent), Fe₃O₄ nanoparticles, and hydroxylated multi-walled carbon nanotubes (an adsorbent). Upon immersion, CO₂ bubbles promote rapid dispersion of reagents and in-situ assembly of a magnetic composite adsorbent, enabling simultaneous derivatization and efficient extraction of sulfonamides. The core tablet-mediated procedure requires only 4 min, with desorption achieved in 0.5 min using acetone. Validation demonstrated excellent linearity (R² > 0.998), low detection limits (0.130–0.285 ng/g), and recoveries of 82.6–107.0% with RSDs <10%. Application to spiked honey confirmed accuracy, with relative errors ranging from −8.9% to +9.2%. Comparative studies showed performance equivalent to conventional multi-step protocols, while reducing handling steps and solvent use during the extraction phase.

Significance

This integrated effervescence/derivatization/extraction strategy streamlines sulfonamide residue analysis into a rapid, cost-effective, and easily automatable workflow. While sample pretreatment is still required for complex matrices, the tablet-based step significantly reduces manual handling and solvent consumption during the extraction and derivatization process. Its compatibility with LC-FLD provides a practical and cost-effective alternative to LC-MS, supporting reliable food safety surveillance within regulatory standards.

磺胺是广泛使用的抗菌剂，但食品中的残留会增加健康风险，如过敏、抗菌素耐药性和致癌性。严格的残留限量要求采用灵敏、高效的监测方法。虽然LC-FLD具有选择性和实用性，但传统的工作流程依赖于多步提取和衍生，这非常耗时。在复杂食品基质中进行实际的残留分析需要一种将提取和衍生化整合到单一操作中的流线型策略。结果研制出一种新型多功能泡腾片，使泡腾片的起泡、衍生、提取一步走。该片剂含有泡腾前体（Na2CO3/NaH2PO4）、荧光胺（衍生化试剂）、Fe3O4纳米颗粒和羟基化多壁碳纳米管（吸附剂）。浸泡后，CO2气泡促进试剂的快速分散和磁性复合吸附剂的原位组装，从而实现磺胺类化合物的同时衍生化和高效提取。核心片介导的过程只需要4分钟，使用丙酮在0.5分钟内完成解吸。验证结果表明：线性良好（R2 > 0.998），检出限低（0.130 ~ 0.285 ng/g），加样回收率为82.6 ~ 107.0%,rsd <10%。应用于加标蜂蜜证实了准确性，相对误差范围为-8.9%至+9.2%。对比研究表明，其性能与传统的多步骤方案相当，同时减少了提取阶段的处理步骤和溶剂使用。这种集成的起泡/衍生化/提取策略将磺胺残留分析简化为快速，经济高效且易于自动化的工作流程。虽然复杂的基质仍然需要样品前处理，但基于片剂的步骤显著减少了提取和衍生过程中的人工处理和溶剂消耗。它与LC-FLD的兼容性为LC-MS提供了一种实用且具有成本效益的替代方案，支持在监管标准内可靠的食品安全监测。

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引用次数: 0

A deprotection-oxidation cascade-regulated dual-responsive near-infrared fluorescent probe for individual and sequential detection of H2S/HClO with applications in food safety monitoring 一种脱保护-氧化级联调节双响应近红外荧光探针，用于单独和顺序检测H2S/HClO在食品安全监测中的应用

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-02-03 DOI: 10.1016/j.aca.2026.345191

Jie Yang , Chao Fu , Yicong Zhou , Hua Zhang , Xiangzhi Song , Dandan Li , Chuanxiang Liu

Background

Hydrogen sulfide (H₂S) and hypochlorous acid (HClO), as critical reactive sulfur (RSS) and oxygen (ROS) species, respectively play dual roles in physiological regulation and food safety. While numerous fluorescent probes exist for individual detection of H₂S or HClO, dual-functional probes remain rare despite their significant potential in food safety, environmental monitoring, and biomedicine.

Results

A novel A-π-A (acceptor-π-bridge-acceptor) type near-infrared fluorescent probe, DCIQ-DNBS, was developed for the first time, featuring dual-response mechanisms for individual and sequential detection of H₂S and HClO via H₂S-triggered thiolysis of the 2,4-dinitrobenzenesulfonyl (DNBS) and HClO mediated DNBS-deprotection-oxidation of electron-deficient CC bond cascade oxidation. The probe DCIQ-DNBS demonstrates exceptional selectivity and rapid response times, and offers dual-channel fluorescence signaling with non-overlapping emissions: a near-infrared response at 740 nm for H₂S (LOD, 49 nM) and a short-wavelength yellow emission at 562 nm for HClO (LOD, 40 nM). Practical applications were demonstrated through DCIQ-DNBS loaded portable test strips, which successfully enabled visual monitoring of gaseous H₂S in food spoilage samples and quantitative tracing of HClO in foods. Furthermore, cellular studies revealed the probe's capability to detect elevated levels of both H₂S and HClO in HeLa cells.

Significance and novelty

This novel single-probe system enables the individual and sequential detection of two distinct analytes in a single assay. By generating unique emission signals for each target, it eliminates the need for separate tests or complex separation steps, thereby revolutionizing efficiency in fields like food safety monitoring.

硫化氢（H2S）和次氯酸（HClO）作为关键的活性硫（RSS）和活性氧（ROS）物质，分别在生理调节和食品安全中发挥着双重作用。虽然目前存在大量用于单独检测H2S或HClO的荧光探针，但双功能探针在食品安全、环境监测和生物医学方面具有巨大的潜力，但仍然很少。结果首次研制了一种新型的A-π-A（受体-π-桥接-受体）型近红外荧光探针DCIQ-DNBS，该探针具有双响应机制，可通过H2S触发2,4-二硝基苯磺酰（DNBS）的硫解和HClO介导的DNBS-脱保护-缺电子C=C键级联氧化对H2S和HClO进行单次和顺序检测。DCIQ-DNBS探针表现出卓越的选择性和快速的响应时间，并提供双通道荧光信号，无重叠发射：对H2S （LOD, 49 nm）有740 nm的近红外响应，对HClO （LOD, 40 nm）有562 nm的短波长黄色发射。通过装载DCIQ-DNBS的便携式试纸条演示了实际应用，成功实现了食品腐败样品中气态H2S的可视化监测和食品中HClO的定量溯源。此外，细胞研究表明，探针能够检测HeLa细胞中H2S和HClO水平的升高。意义和新颖性这种新颖的单探针系统能够在一次分析中对两种不同的分析物进行单独和顺序的检测。通过为每个目标产生独特的发射信号，它消除了单独测试或复杂分离步骤的需要，从而彻底提高了食品安全监测等领域的效率。

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引用次数: 0

Aptamer Modified NPA Hollow Nanozymes with Intrinsic Peroxidase-Like Activity for Colorimetric Sensing of H1N1 具有内在过氧化物酶样活性的核酸适体修饰的NPA中空纳米酶用于H1N1的比色检测

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-02-02 DOI: 10.1016/j.aca.2026.345189

Jianjun Wang, Shuyuan Zhang, Qingqing Wang, Haijiang Gong, Boyu Chen, Qi Liu, Lili Feng, Shili Gai, Piaoping Yang

A colorimetric sensor based on aptamer-functionalized nanozyme probes was successfully developed for the sensitive and specific detection of influenza A (H1N1) virus. In this study, trimetallic nickel-palladium-gold hollow nanozymes (NPA) with excellent peroxidase (POD)-like activity were used as catalytic probes. Compared to the horseradish peroxidase (HRP), the NPA nanozyme exhibits superior peroxidase-like activity, demonstrating a lower Michaelis constant (K_m) and a higher maximum reaction rate (V_max) for the substrates TMB and H₂O₂, indicating enhanced substrate affinity. Via the gold-sulfur bond interaction, thiol-modified aptamers that could specifically recognize H1N1 hemagglutinin (HA) were immobilized on the nanozyme surface, enabling accurate HA recognition under two detection methods: (1) For the colorimetric biosensor, the binding of HA to the aptamer formed an interfacial steric hindrance layer on the nanozyme surface, which inhibited the catalytic reaction, leading to a significant decrease in absorbance; (2) In the modified ELISA, the "antibody-HA-aptamer" sandwich structure enriched nanozymes at the detection interface, and a color signal enhancement correlated with HA concentration was observed after adding the chromogenic substrate. Experimental results showed that the dual-mode method achieved linear detection ranges of 2–250 ng·mL^-1 and 2–62.5 ng·mL^-1 for HA protein, with limits of detection (LODs) as low as 1.5 ng·mL^-1 and 1.2 ng·mL^-1, respectively. This method also exhibited good specificity against other influenza virus subtypes (e.g., H5N1) and was successfully applied to the detection of inactivated H1N1 virus in serum samples. Furthermore, by integrating a smartphone-based colorimetric analysis system, demonstrating its potential for rapid and on-site detection of the H1N1 virus. This work provides a new insight for the development of target-specific nanozyme sensors.

成功研制了一种基于适体功能化纳米酶探针的比色传感器，用于甲型H1N1流感病毒的灵敏和特异性检测。本研究以具有优异过氧化物酶（POD）样活性的三金属镍钯金空心纳米酶（NPA）作为催化探针。与辣根过氧化物酶（HRP）相比，NPA纳米酶表现出更强的过氧化物酶样活性，对底物TMB和H2O2具有更低的米切里斯常数（Km）和更高的最大反应速率（Vmax），表明其对底物的亲和力增强。通过金-硫键相互作用，将巯基修饰的能特异性识别H1N1血凝素（HA）的适体固定在纳米酶表面，通过两种检测方法实现对HA的准确识别：(1)比色生物传感器，HA与适体的结合在纳米酶表面形成界面位阻层，抑制了催化反应，导致吸光度显著降低；(2)在改进后的ELISA中，“抗体-HA-适体”三明治结构在检测界面富集了纳米酶，添加显色底物后观察到与HA浓度相关的颜色信号增强。实验结果表明，双模法对HA蛋白的线性检测范围为2 ~ 250 ng·mL-1和2 ~ 62.5 ng·mL-1，检出限（lod）分别低至1.5 ng·mL-1和1.2 ng·mL-1。该方法对其他流感病毒亚型（如H5N1）也表现出良好的特异性，并成功应用于血清样品中灭活H1N1病毒的检测。此外，通过集成基于智能手机的比色分析系统，展示了其快速和现场检测H1N1病毒的潜力。这项工作为靶向纳米酶传感器的发展提供了新的见解。

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引用次数: 0

·ATP-enhanced PB@MIL-100(Fe) nanozyme enables dual-mode ratiometric colorimetric and smartphone-based hydrogel detection of nitrite in food samples ·atp增强PB@MIL-100（Fe）纳米酶可实现食品样品中亚硝酸盐的双模式比例比色法和基于智能手机的水凝胶检测

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-02-02 DOI: 10.1016/j.aca.2026.345192

Biyao Dai , Quanyong Dong , Taiqi Hao , Shenghui Zhang , Dong Xu , Ying Xiong , Wang Li , Qian Wen , Zhimei Huang , Jiali Ren

Background

Nitrite residues pose significant threats to food safety, as excessive consumption of this may cause serious health risks to human. Conventional techniques such as Griess reagent and chromatographic methods can successfully quantify nitrite levels, but these approaches often suffer from complex sample preparation, poor selectivity, expensive instrumentation, necessitate professional operators and time-consuming procedures. These limitations restrict their practical application for detection nitrites in point-of-care testing (POCT). Therefore, developing sensitive and portable nitrite detection methods remains critically important for food safety monitoring.

Results

Herein, we developed a novel dual-mode sensing platform based on composite nanozymes PB@MIL-100(Fe)@ATP for sensitive, selective and portable nitrite detection. This platform integrates Prussian Blue with MIL-100(Fe) metal-organic framework and exploits adenosine triphosphate (ATP) as an enhancer. The resulting PB@MIL-100(Fe)@ATP demonstrated excellent peroxidase-like (POD-like) catalytic activity, which catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue-colored oxidized TMB (TMBox) with a characteristic absorption peak at 652 nm. In the presence of nitrites, TMBox specifically undergoes diazotization under acidic conditions to produce yellow diazotized TMB, exhibiting increased absorbance at 450 nm while decreasing at 652 nm. The ratiometric colorimetric assay achieved a detection limit of 0.17 μM within 20 min through absorbance ratio analysis at 652/450 nm. Furthermore, a smartphone-assisted hydrogel sensing method with detection limit of 1.68 μM was developed for point-of-care nitrites monitoring through image capture and color analysis, providing equipment-free operation.

Significance

The ATP-enhanced PB@MIL-100(Fe) nanozyme was successfully applied to detect nitrites with high recovery rates in real pickled vegetables, sauerkraut, and sausage samples. This dual-mode platform demonstrated excellent selectivity, high quantitative accuracy, and practical applicability in complex food matrices, providing a simple, rapid and versatile tool for on-site food safety monitoring.

亚硝酸盐残留对食品安全构成重大威胁，过量食用会对人体健康造成严重危害。Griess试剂和色谱法等传统技术可以成功地定量亚硝酸盐水平，但这些方法通常存在样品制备复杂、选择性差、仪器昂贵、需要专业操作人员和耗时的过程等问题。这些局限性限制了它们在即时检测（POCT）中检测亚硝酸盐的实际应用。因此，开发灵敏、便携的亚硝酸盐检测方法对食品安全监测至关重要。结果建立了一种基于复合纳米酶PB@MIL-100(Fe)@ATP的新型双模传感平台，用于亚硝酸盐的灵敏、选择性和便携式检测。该平台将普鲁士蓝与MIL-100（Fe）金属有机框架相结合，利用三磷酸腺苷（ATP）作为增强剂。得到的PB@MIL-100(Fe)@ATP具有优异的过氧化物酶样（pod样）催化活性，催化3,3',5,5'-四甲基联苯胺（TMB）氧化生成蓝色氧化TMB (TMBox)，其特征吸收峰在652nm处。在亚硝酸盐的存在下，TMBox在酸性条件下特异性重氮化，生成黄色重氮化的TMB，在450nm处吸光度增加，在652nm处吸光度下降。通过652/450 nm吸光度分析，比值比色法在20分钟内检出限为0.17 μM。此外，开发了一种检测限为1.68 μM的智能手机辅助水凝胶传感方法，通过图像采集和颜色分析进行即时亚硝酸盐监测，无需设备操作。意义atp增强PB@MIL-100（Fe）纳米酶可用于实际腌菜、酸菜和香肠样品中亚硝酸盐的检测，回收率高。该双模平台在复杂的食品基质中表现出优异的选择性、高的定量准确性和实用性，为现场食品安全监测提供了一种简单、快速、通用的工具。

{"title":"·ATP-enhanced PB@MIL-100(Fe) nanozyme enables dual-mode ratiometric colorimetric and smartphone-based hydrogel detection of nitrite in food samples","authors":"Biyao Dai , Quanyong Dong , Taiqi Hao , Shenghui Zhang , Dong Xu , Ying Xiong , Wang Li , Qian Wen , Zhimei Huang , Jiali Ren","doi":"10.1016/j.aca.2026.345192","DOIUrl":"10.1016/j.aca.2026.345192","url":null,"abstract":"<div><h3>Background</h3><div>Nitrite residues pose significant threats to food safety, as excessive consumption of this may cause serious health risks to human. Conventional techniques such as Griess reagent and chromatographic methods can successfully quantify nitrite levels, but these approaches often suffer from complex sample preparation, poor selectivity, expensive instrumentation, necessitate professional operators and time-consuming procedures. These limitations restrict their practical application for detection nitrites in point-of-care testing (POCT). Therefore, developing sensitive and portable nitrite detection methods remains critically important for food safety monitoring.</div></div><div><h3>Results</h3><div>Herein, we developed a novel dual-mode sensing platform based on composite nanozymes PB@MIL-100(Fe)@ATP for sensitive, selective and portable nitrite detection. This platform integrates Prussian Blue with MIL-100(Fe) metal-organic framework and exploits adenosine triphosphate (ATP) as an enhancer. The resulting PB@MIL-100(Fe)@ATP demonstrated excellent peroxidase-like (POD-like) catalytic activity, which catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue-colored oxidized TMB (TMBox) with a characteristic absorption peak at 652 nm. In the presence of nitrites, TMBox specifically undergoes diazotization under acidic conditions to produce yellow diazotized TMB, exhibiting increased absorbance at 450 nm while decreasing at 652 nm. The ratiometric colorimetric assay achieved a detection limit of 0.17 μM within 20 min through absorbance ratio analysis at 652/450 nm. Furthermore, a smartphone-assisted hydrogel sensing method with detection limit of 1.68 μM was developed for point-of-care nitrites monitoring through image capture and color analysis, providing equipment-free operation.</div></div><div><h3>Significance</h3><div>The ATP-enhanced PB@MIL-100(Fe) nanozyme was successfully applied to detect nitrites with high recovery rates in real pickled vegetables, sauerkraut, and sausage samples. This dual-mode platform demonstrated excellent selectivity, high quantitative accuracy, and practical applicability in complex food matrices, providing a simple, rapid and versatile tool for on-site food safety monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345192"},"PeriodicalIF":6.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Protease-Resistant and Anti-Fouling Electrochemical Biosensing Interface Constructed with DL-Serine Modified Y-Shaped Peptides for Reliable Analysis of Aminopeptidase N in Human Serum 用dl -丝氨酸修饰的y型多肽构建抗蛋白酶抗污垢电化学生物传感界面，可靠分析人血清中氨基肽酶N

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-31 DOI: 10.1016/j.aca.2026.345185

Yudong Wang, Yanxin Li, Yinan Zhan, Jie Yang, Yuxin Zhang, Lei Wang, Xiliang Luo

引用次数: 0

Understanding sub-cellular mechanochemical correlations of the neonatal extrahepatic bile duct extracellular matrix by atomic force microscope-Infrared spectroscopy 原子力显微镜-红外光谱法研究新生儿肝外胆管细胞外基质的亚细胞力学化学关系

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-31 DOI: 10.1016/j.aca.2026.345175

Huiyong Li, Kapish Gupta, Zhenqin Wang, Jessica Llewellyn, Hanxun Jin, Samuel Tenney, Rebecca G. Wells, Marcus Foston

引用次数: 0

CuAg NCs with AIE Effect Coordinated by Ce3+ as a Novel Fluorescent Probe for the screening of ATP inhibiting drugs Ce3+配位AIE效应CuAg NCs作为筛选ATP抑制药物的新型荧光探针

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-31 DOI: 10.1016/j.aca.2026.345170

Jinwen Chen, Huixiao Tong, Yuqing Wang, Yinghui Dang, Ziheng Gu, Ruijue Liu, Shenghua Liao

Background

In recent years, bimetallic nanoclusters (NCs) have emerged as promising functional materials, particularly those exhibiting aggregation-induced emission (AIE) properties. Compared to single-metal systems or organic AIE fluorophores, they offer enhanced fluorescence, superior stability, straightforward synthesis, and excellent biocompatibility. This has enabled them to attract considerable attention in the field of chemical sensing and bioimaging. Specifically, adenosine triphosphate (ATP) serves as a critical biomarker, and the development of sensitive fluorescent probes for its detection is of significant importance in biomedical research and clinical diagnostics.

Results

This study developed a mitochondrial-targeted fluorescent probe based on Ce³⁺- coordinated copper–silver bimetallic nanoclusters (CuAg NCs), designed for selective ATP recognition and ATP-inhibiting drugs screening. The AIE-active CuAg NCs were synthesized via a facile approach using L-glutathione (GSH) as both reducing agent and stabilizing ligand. Upon addition of Ce³⁺, the quantum yields (QYs) increased from 0.60% to 22.64%. This attributed to the suppresion of non-radiative relaxation pathways in the excited states through coordination interaction between Ce³⁺ and carboxyl groups (-COOH) of surface-anchored GSH ligands. Leveraging the coordination between Ce³⁺ and the triphosphate moiety of ATP, a turn-off fluorescent probe was constructed, demonstrating a linear response within 1–30 μM and a detection limit of 0.105 μM. Owing to its biocompatibility and inherent mitochondrial-targeting ability, it enabled effective intracellular ATP monitoring. In HepG2 cells, fluorescence intensity showed clear dose-dependence upon treatment with ATP-activator (Glimepiride) and ATP-inhibitor (Imatinib).

Significance and Novelty

This work not only presents a sensitive and selective probe for ATP determination but also establishes a versatile platform for real-time tracking of intracellular ATP levels and screening of ATP-modulating drugs. The proposed design strategy based on metal-coordination enhanced bimetallic NCs offers a novel pathway for developing organelle-targeted functional probes, highlighting its potential in biomedical sensing and pharmacological research.

近年来，双金属纳米团簇（NCs）已成为一种很有前途的功能材料，特别是那些具有聚集诱导发射（AIE）特性的材料。与单金属系统或有机AIE荧光团相比，它们具有增强的荧光，优越的稳定性，简单的合成和出色的生物相容性。这使它们在化学传感和生物成像领域引起了相当大的注意。具体来说，三磷酸腺苷（adenosine triphosphate， ATP）是一种重要的生物标志物，开发灵敏的荧光探针检测其在生物医学研究和临床诊断中具有重要意义。结果建立了一种基于Ce3+配位铜银双金属纳米簇（CuAg NCs）的线粒体靶向荧光探针，用于选择性ATP识别和ATP抑制药物筛选。以l -谷胱甘肽（GSH）为还原剂和稳定配体，通过简便的方法合成了具有aie活性的CuAg NCs。Ce3+的加入使量子产率由0.60%提高到22.64%。这归因于Ce3+与表面锚定GSH配体的羧基（-COOH）之间的配位相互作用抑制了激发态中的非辐射弛豫途径。利用Ce3+与ATP的三磷酸基团之间的配合，构建了一个关闭荧光探针，在1 ~ 30 μM范围内具有线性响应，检测限为0.105 μM。由于其生物相容性和固有的线粒体靶向能力，它可以有效地监测细胞内ATP。在HepG2细胞中，荧光强度对atp激活剂（格列美脲）和atp抑制剂（伊马替尼）的治疗表现出明显的剂量依赖性。意义与创新本研究不仅提供了一种灵敏、选择性的ATP检测探针，而且为实时跟踪细胞内ATP水平和筛选ATP调节药物建立了一个多功能平台。提出的基于金属配位增强双金属NCs的设计策略为开发细胞器靶向功能探针提供了新的途径，突出了其在生物医学传感和药理学研究中的潜力。

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引用次数: 0

In situ imaging of oxidation dynamics within aluminum laser induced plasmas 铝激光诱导等离子体内氧化动力学的原位成像

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-31 DOI: 10.1016/j.aca.2026.345177

Justin I. Borrero-Negrón, Kyle C. Hartig

引用次数: 0

High-Performance Liquid Chromatography Enables Purification of Red-Emitting Carbon Dots for Cellular Imaging 高效液相色谱使细胞成像的红色发射碳点纯化成为可能

IF 6.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-31 DOI: 10.1016/j.aca.2026.345176

Manivannan Madhu, Sih-Ying Chen, Po-Chiao Lin, Wei-Lung Tseng

引用次数: 0

Unlocking the complexity of macromolecular protein heterogeneity of glycoprotein IgE via an optimized online digestion middle-down proteomics approach 通过优化的在线消化中下蛋白质组学方法揭示糖蛋白IgE大分子蛋白异质性的复杂性

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2026-01-30 DOI: 10.1016/j.aca.2026.345161

Ruijie Liu , Zixin Liang , Shujun Xia , Yuxiang Luo , Huilin Li

Background

Proteoform-level analysis of large, highly modified proteins (≥50 kDa) such as immunoglobulin E (IgE) remains challenging due to their size and molecular heterogeneity. While traditional bottom-up proteomics fails to retain proteoform-level information, top-down approaches often lack the resolution required for effective proteoform discrimination. Middle-down proteomics (MDP) overcomes these limitations by generating highly redundant peptides in the 3–15 kDa range, which retain critical combinatorial modification patterns, thereby enabling more comprehensive characterization of complex proteoforms.

Results

To advance heterogeneity analysis of large proteins, we developed an online, tunable pepsin digestion platform that facilitates rapid and in-depth middle-down analysis of large proteins. When applied to IgE—a key allergy-related antibody with a large molecular weight (170–190 kDa) and bearing seven potential N-glycosylation sites—this platform produces high-redundancy peptides, enabling de novo sequencing of the variable region, which is critical for antigen recognition. Furthermore, analysis of middle-sized peptides allowed us to detect combined glycosylation patterns on the constant region 3 of the epsilon heavy chain (Cε3), revealing 39 Cε3 domain proteoforms. These proteoforms are important for Fcε receptor I interaction, therapeutic antibody binding, and downstream signaling. The platform also provided comprehensive glycosylation insights, including composition, occupancy, and microheterogeneity, thereby supporting detailed annotation of intact IgE proteoforms with varying glycan numbers.

Significance

By integrating sequence and glycosylation information, our approach offers a holistic perspective of IgE heterogeneity, which is essential for elucidating their roles in allergic diseases and for guiding diagnostic and therapeutic development. This study demonstrates that MDP analysis is highly valuable for large, diverse proteins like IgE. Our workflows enhance methodological versatility by minimizing dependence on specific proteases for particular proteins, thereby broadening the potential applications of MDP.

大的、高度修饰的蛋白（≥50 kDa），如免疫球蛋白E (IgE)，由于其大小和分子异质性，在蛋白形态水平上的分析仍然具有挑战性。传统的自底向上的蛋白质组学无法保留蛋白质水平的信息，而自顶向下的方法往往缺乏有效区分蛋白质所需的分辨率。中下蛋白质组学（mid -down proteomics， MDP）通过生成3-15 kDa范围内的高度冗余肽来克服这些限制，这些肽保留了关键的组合修饰模式，从而能够更全面地表征复杂的蛋白质形态。结果为了推进大蛋白的异质性分析，我们开发了一个在线、可调的胃蛋白酶消化平台，促进了对大蛋白的快速、深入的中向下分析。当应用于ige（一种关键的过敏相关抗体，分子量大（170-190 kDa），含有7个潜在的n -糖基化位点）时，该平台产生高冗余肽，使可变区域的重新测序成为可能，这对抗原识别至关重要。此外，对中等大小多肽的分析使我们能够检测到epsilon重链恒定区3 （Cε3）上的组合糖基化模式，揭示了39种Cε3结构域蛋白形式。这些蛋白形式对于fce1受体相互作用、治疗性抗体结合和下游信号传导都很重要。该平台还提供了全面的糖基化见解，包括组成、占用和微异质性，从而支持对具有不同聚糖数的完整IgE蛋白形式的详细注释。通过整合序列和糖基化信息，我们的方法提供了IgE异质性的整体视角，这对于阐明它们在过敏性疾病中的作用以及指导诊断和治疗发展至关重要。这项研究表明，MDP分析对于像IgE这样的大而多样的蛋白质非常有价值。我们的工作流程通过最大限度地减少对特定蛋白质的特定蛋白酶的依赖来增强方法的通用性，从而扩大了MDP的潜在应用。

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引用次数: 0