Analytica Chimica Acta最新文献

{"title":"Permanganate Assisted Oxidative-Chlorination Digestion for Trace Determination of Gold in Geological Samples by GF AAS","authors":"Gourab Rana, Abheek Sarkar","doi":"10.1016/j.aca.2026.345424","DOIUrl":"https://doi.org/10.1016/j.aca.2026.345424","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"17 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147496524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent POCT for Ovarian Cancer: Dual-Biomarker Detection Combined with ROMA Risk Assessment Using Selenium Nanoparticle","authors":"Weiqi Xie, Jinbo Wu, Xiaoyi Xie, Mengli Wu, Menghan Zhang, Mengyao Zhang, Xiaoquan Li, Li Wang, Lanju Wang, Zhigang Liu, Zhizeng Wang, Chunxia Chen","doi":"10.1016/j.aca.2026.345434","DOIUrl":"https://doi.org/10.1016/j.aca.2026.345434","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"38 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A near-infrared ratiometric fluorescent probe for visual sensing of H2S and monitoring its fluctuation in plant roots under drought and flooding stresses","authors":"Ruxuan Mo ,&nbsp;Chao Kang ,&nbsp;Jianping Yuan ,&nbsp;Carl Redshaw ,&nbsp;Ying Huang ,&nbsp;Qilong Zhang ,&nbsp;Xiufang Yan","doi":"10.1016/j.aca.2026.345144","DOIUrl":"10.1016/j.aca.2026.345144","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogen sulfide (H₂S) is a key endogenous gasotransmitter involved in plant physiological regulation and stress responses. Monitoring its dynamic changes in plants is essential for understanding related signaling mechanisms. However, due to its chemical instability and the complexity of plant tissues, developing a reliable method for accurate quantification and real-time visualization of H₂S in living plants remains challenging. Although fluorescent probes have been developed for H₂S imaging, most still face critical limitations, such as emission in the visible region being susceptible to background fluorescence interference, and insufficient quantitative reliability of single-wavelength-based signal output modes in complex samples. Therefore, developing novel probes with near-infrared emission and rationetric response characteristics is of great significance.</div></div><div><h3>Results</h3><div>We constructed a near-infrared rationetric fluorescent probe, NIR-Cou-H₂S, for H₂S detection. The probe itself emits at 716 nm, and after specific reaction with H₂S, a new emission peak appears at 552 nm, resulting in a distinct dual-emission rationetric response (716 nm/552 nm) accompanied by a visible color change. Using chemometrics-based fluorescence analysis, the probe successfully enabled direct quantitative detection of H₂S in river and lake water samples. Its near-infrared emission effectively reduced interference from plant autofluorescence, thereby achieving high-contrast dual-channel fluorescence imaging. The probe was successfully applied for high-quality in situ visualization of H₂S in living cells and tobacco seedling roots. More importantly, using NIR-Cou-H₂S, we observed and recorded in real time the dynamic upregulation trend of endogenous H₂S levels in tobacco roots under both drought and flooding stress conditions.</div></div><div><h3>Significance</h3><div>As a novel near-infrared rationetric probe, NIR-Cou-H₂S provides a powerful tool for monitoring endogenous H₂S dynamics in plants. Its characteristics significantly improve the reliability of imaging and quantification in complex plant samples, offering a key methodological approach for further elucidating the regulatory mechanisms of H₂S in plant stress resistance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345144"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A protease-resistant and anti-fouling electrochemical biosensing interface constructed with dl-serine modified Y-shaped peptides for reliable analysis of aminopeptidase N in human serum","authors":"Yudong Wang,&nbsp;Yanxin Li,&nbsp;Yinan Zhan,&nbsp;Jie Yang,&nbsp;Yuxin Zhang,&nbsp;Lei Wang,&nbsp;Xiliang Luo","doi":"10.1016/j.aca.2026.345185","DOIUrl":"10.1016/j.aca.2026.345185","url":null,"abstract":"<div><div>Electrochemical sensing techniques are still facing the challenges of biofouling and insufficient probe stability in complex biological matrices. To address this issue, we have designed a <span>dl</span>-serine Y-shaped peptide (YPEP) integrating the antifouling properties and recognition efficiency. Application demonstration was performed with detecting aminopeptidase N (APN) in human serum. The peptide-modified sensing interface exhibits effective antifouling performance, and enhanced resistance to enzymatic degradation. When detecting APN, the developed sensor showed a sensitive response within the linear range of 1.0 ng mL⁻¹ to 10 μg mL⁻¹ and a detection limit of 0.24 ng mL⁻¹. Comparison studies were performed with the clinical human serum samples, and results obtained with the developed sensor and standard ELISA methods are in close agreement. This work provides a feasible strategy to developing robust antifouling peptide-based biosensing platforms for clinical applications in complex biological matrices.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345185"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146095760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histology-guided spatial lipidomics and proteomics of the trisynaptic circuit in the human hippocampus","authors":"Caitlin M. Tressler ,&nbsp;Lauren DeVine ,&nbsp;Rahul Bharadwaj ,&nbsp;Dalton R. Brown ,&nbsp;Daniel Weinberger ,&nbsp;Kristine Glunde ,&nbsp;Robert N. Cole","doi":"10.1016/j.aca.2026.345110","DOIUrl":"10.1016/j.aca.2026.345110","url":null,"abstract":"<div><div>The human hippocampal trisynaptic circuit activity is essential for learning and memory. This canonical circuit has spatially distinct populations of neurons, but their unique contributions to neurodevelopment, as well as to dysfunction in neurodegenerative disorders, are missed when analyzing bulk tissue homogenates. Using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to guide laser capture microdissection (LCMD) of regions of interest for spatial multimodal analyses is a relatively new approach to study topographically distinct neuronal cell populations in heterogenous tissues. However, MALDI-MSI may not identify region-defining molecular mass-to-charge ions. Here, we apply a multimodal approach of MALDI-MSI-LCMD-lipidomic and proteomic analysis to the trisynaptic circuit. Our MALDI-MSI of the hippocampus revealed that the of mass-to-charge ions of the cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3) did not segment from the surrounding tissue. Thus, we developed a novel histology-guided MALDI-MS imaging-LCMD-spatial lipidomic/proteomic pipeline with four steps which does not rely on segmentation analysis to determine and co-register regions of interest in tissue sections. Our pipeline allows MALDI imaging, LCMD, lipidomic and proteomic analysis from the same tissue section and does not require co-registration across serial sections. In addition, poly-<span>l</span>-lysine coating for improving tissue/cell adherence on indium-tin-oxide microscopy slides did not impact MALDI-MSI or spatial proteomics. We show that the human trisynaptic circuit proteomes of CA1 and CA3 pyramidal neurons are more similar to each other than those of the dentate gyrus (DG), which is consistent with previously reported transcriptomics studies. The spatial distributions of several phospholipids and proteins, however, were significantly different in cell bodies from the CA1, CA3 and DG regions, and these lipids correlated with some lipid metabolizing enzymes in those regions. As little is known about lipid metabolism in the hippocampus, our pipeline provides an initial step in studying the combined and differential spatial regulation of the lipids and proteins within the trisynaptic circuit that will provide insights into the development and disease-related molecular changes in these important hippocampal regions.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345110"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146033809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicking-controlled biosensor: Integrating DNA Walker-RCA circuit with trimetallic nanozyme for aflatoxin B1 detection","authors":"Longsheng Jin ,&nbsp;YuJing Liu ,&nbsp;Wenqing Xia ,&nbsp;Meiling Zhou ,&nbsp;Ziying Xiao ,&nbsp;Jingyu Ge ,&nbsp;Xiao Yuan ,&nbsp;Qinqiang Zhu ,&nbsp;Meisheng Wu","doi":"10.1016/j.aca.2026.345193","DOIUrl":"10.1016/j.aca.2026.345193","url":null,"abstract":"<div><h3>Background</h3><div>Aflatoxin B₁ (AFB₁) is a highly toxic mycotoxin that poses serious threats to global food safety and public health. The ingestion of these contaminated products causes substantial risks to both food safety systems and public health. Despite the availability of various detection techniques, the requirement for highly sensitive analysis of AFB₁ contamination in agricultural products continues to drive demand for improved methodologies.</div></div><div><h3>Results</h3><div>In this work, a novel electrochemiluminescence (ECL) biosensor was developed for the sensitive detection of aflatoxin B₁ (AFB₁) by integrating a galvanic replacement–synthesized Au@Ag–Pt nanozyme with a DNA walker–controlled rolling circle amplification (RCA) circuit. The core–shell Au@Ag–Pt nanozyme exhibits superior conductivity, enhanced peroxidase activity, and electrocatalytic performance, leading to a 17.67–fold amplification of ECL. A target-responsive “signal–off” mechanism was established based on AFB₁-induced DNA structural switching and enzymatic cleavage, which effectively suppresses the RCA process and reduces the immobilization of nanozyme labels. The biosensor achieved a wide linear range from 5 × 10⁻¹¹ to 5 × 10⁻⁵ mg/mL with a detection limit of 2.72 × 10⁻¹¹ mg/mL. It shows high specificity against common interfering mycotoxins, good reproducibility, and satisfactory recoveries ranging from 82.7% to 110.5%.</div></div><div><h3>Significance</h3><div>This work not only provides a reliable platform for mycotoxin monitoring but also advances nanozyme engineering in biosensing, offering significant promise for food safety and environmental applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345193"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed impurity profiling of graphene oxide fractions by robust inductively coupled plasma optical emission spectrometry monitoring","authors":"Iuliia A. Poimenova ,&nbsp;Elizaveta Yu. Zhukova ,&nbsp;Svetlana T. Ovseenko ,&nbsp;Nikita S. Saratovsky ,&nbsp;Ivan K. Kiryukhin ,&nbsp;Daria-Mariia V. Ratova ,&nbsp;Dmitrii M. Filatov ,&nbsp;Anastasia M. Alekseeva ,&nbsp;Svetlana V. Smirnova ,&nbsp;Mikhail A. Proskurnin ,&nbsp;Ivan V. Mikheev","doi":"10.1016/j.aca.2026.345140","DOIUrl":"10.1016/j.aca.2026.345140","url":null,"abstract":"<div><h3>Background</h3><div>Graphene oxide (GO) is widely used in biomedicine and biotechnology due to its aqueous dispersibility and potential nanozyme activity. However, it remains analytically challenging because of structural heterogeneity and metallic inclusions, which affect its functional performance. GO therefore requires precise trace metal analysis, as even minor compositional variations can be critical. Modern multi-element analysis demands results that are both reliable and accurate, yet sound laboratory practice is not always followed. Although ICP-OES offers high sensitivity and precision, metrological rigor is often lacking. This work addresses the need for validated and robust elemental analysis of GO bulk material and particle-size fractions.</div></div><div><h3>Results</h3><div>ICP-OES was employed to quantify metals in bulk GO and to characterize Mn and Ti distribution in GO fractions. A fast algorithm was developed to identify robust plasma conditions by mapping the Mermet coefficient (Mg II/Mg I intensity ratio) across a range of flow rates and RF power levels. Concentrations for selected elements in the bulk material ranged from 10⁻¹ to 10³ mg kg⁻¹; in aqueous dispersions, values reached up to 10³ mg L⁻¹. Validation parameters for Mn, Ti, and Fe included recoveries of 85–115 %, detection limits of 0.1–0.3 ng kg⁻¹ (for used weight ca. 50 mg), and intra-/inter-day RSDs not exceeding 7 %. The Mermet coefficient exhibited an RSD of ∼0.6 %, indicating minimal matrix interference. Fraction analysis revealed a monotonic decrease in Mn toward fractions with smaller lateral-size cut-offs. ATR-FTIR spectra were obtained for stirred, purified, and ultrasound-treated GO fractions to assess whether purification altered the surface functional groups.</div></div><div><h3>Significance</h3><div>This study provides a validated approach for trace metal analysis in GO, combining sensitivity, robustness, and reproducibility across both bulk solid and aqueous dispersion forms. By experimentally examining the influence of plasma conditions on analytical performance, it offers the first systematic assessment of GO-specific ICP-OES parameter optimization. The results support more reliable characterization of GO materials and enable improved quality control in applications where elemental composition critically affects functionality.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345140"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-esterification preparation of carboxyl groups enriched magnetic microporous organic network for the efficient enrichment and determination of quaternary ammonium alkaloids","authors":"Jian-Ming Liu ,&nbsp;Jian Liu ,&nbsp;Tian-Tian Ma ,&nbsp;Cheng-Xiong Yang","doi":"10.1016/j.aca.2026.345173","DOIUrl":"10.1016/j.aca.2026.345173","url":null,"abstract":"<div><h3>Background</h3><div>Microporous organic networks (MONs) are an emerging class of porous materials characterized by extremely large surface area, tunable topology, and superior stabilities, which have received increasing attention in sample pretreatment. However, their superhydrophobic nature has largely limited their application scope. The synthesis of functionalized MONs has proved to be a feasible strategy to broaden their utility. Nevertheless, this approach remains constrained by the limited variety and availability of functionalized monomers. Therefore, developing novel post-synthetic modification strategies is crucial for expanding the functionality and applications of MONs, particularly in trace analyte enrichment.</div></div><div><h3>Results</h3><div>Herein, we present a novel post-esterification strategy to synthesize a new carboxyl groups enriched magnetic MON (MMON-2COOH) for the highly efficient magnetic solid-phase extraction (MSPE) of four quaternary ammonium alkaloids (QAAs) from complex urine samples. The resulting MMON-2COOH not only retains the porous structure, excellent solvent and thermal stability, and rapid magnetic responsiveness of its precursor (hydroxyl-functionalized magnetic MON) but also incorporates conjugated networks and abundant carboxyl groups. This enables efficient extraction of QAAs through multiple interactions, including π-π stacking, hydrogen bonding, and electrostatic attraction. After optimization of extraction factors, an analytical method integrating MMON-2COOH-based extraction with HPLC-UV detection was established, achieving sensitive and selective determination of QAAs with low limits of detection of 0.05–0.5 μg L⁻¹ and limits of quantification of 0.5–1.0 μg L⁻¹, wide linear range of 0.5–1000 μg L⁻¹, negligible matrix effect of 0.88–1.09, rapid extraction of 2 min, and less adsorbent consumption of 2 mg.</div></div><div><h3>Significance</h3><div>This is the first example of post-esterification preparation of carboxyl groups functionalized MON. The results demonstrate that MMON-2COOH is a highly effective adsorbent, and the developed method is suitable for accurate determination of QAAs in biological samples. This work highlights the significant potential of post-esterification as a versatile approach for designing high-performance adsorbents in sample pretreatment.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345173"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a rapid colorimetric sensor for Microcystin-LR based on specific aptamer and positively charged gold nanoparticles","authors":"Xueting Cui ,&nbsp;Jun Wang ,&nbsp;Tingyu Hou ,&nbsp;Ming Cai ,&nbsp;Luozhao Wang ,&nbsp;Yanna Liu ,&nbsp;Jinhua Li","doi":"10.1016/j.aca.2026.345139","DOIUrl":"10.1016/j.aca.2026.345139","url":null,"abstract":"<div><div>Eutrophication of surface freshwater drinking sources intensifies, leading blue-green algae to release massive microcystins. Microcystin-LR (MC-LR), the most toxic variant, poses severe threats to public health and environmental safety. Conventional MC-LR detection methods rely on bulky, expensive equipment and involve complex, time-consuming sample pretreatment, making them unsuitable for on-site testing. This study developed a colorimetric sensing platform based on the binding of positively charged gold nanoparticles (AuNPs) to MC-LR aptamers for simple and rapid MC-LR detection. Without MC-LR, positively charged gold nanoparticles bind to negatively charged aptamers, aggregate, and turn the solution from yellow to white. This method exhibits high sensitivity (detection limit: 0.1 μg/L) and good specificity (insensitive to interferents like MC-YR and MC-RR), with a total assay time of 55 min, providing a rapid alternative to conventional laboratory-based methods like ELISA. Tests on actual water samples showed recoveries of 96.1–119.8% with relative standard deviations &lt;10%, demonstrating good accuracy and precision for real environmental water analysis. The method's performance suggests its potential for on-site screening, although some variability was observed in complex matrices. This visualized assay eliminates reliance on large instruments, shortens detection time, and holds great potential for environmental water sample testing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345139"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CuAg NCs with AIE Effect Coordinated by Ce3+ as a Novel Fluorescent Probe for the screening of ATP inhibiting drugs","authors":"Jinwen Chen,&nbsp;Huixiao Tong,&nbsp;Yuqing Wang,&nbsp;Yinghui Dang,&nbsp;Ziheng Gu,&nbsp;Ruijue Liu,&nbsp;Shenghua Liao","doi":"10.1016/j.aca.2026.345170","DOIUrl":"10.1016/j.aca.2026.345170","url":null,"abstract":"<div><h3>Background</h3><div>In recent years, bimetallic nanoclusters (NCs) have emerged as promising functional materials, particularly those exhibiting aggregation-induced emission (AIE) properties. Compared to single-metal systems or organic AIE fluorophores, they offer enhanced fluorescence, superior stability, straightforward synthesis, and excellent biocompatibility. This has enabled them to attract considerable attention in the field of chemical sensing and bioimaging. Specifically, adenosine triphosphate (ATP) serves as a critical biomarker, and the development of sensitive fluorescent probes for its detection is of significant importance in biomedical research and clinical diagnostics.</div></div><div><h3>Results</h3><div>This study developed a mitochondrial-targeted fluorescent probe based on Ce³⁺- coordinated copper–silver bimetallic nanoclusters (CuAg NCs), designed for selective ATP recognition and ATP-inhibiting drugs screening. The AIE-active CuAg NCs were synthesized via a facile approach using <span>l</span>-glutathione (GSH) as both reducing agent and stabilizing ligand. Upon addition of Ce³⁺, the quantum yields (QYs) increased from 0.60% to 22.64%. This attributed to the suppression of non-radiative relaxation pathways in the excited states through coordination interaction between Ce³⁺ and carboxyl groups (-COOH) of surface-anchored GSH ligands. Leveraging the coordination between Ce³⁺ and the triphosphate moiety of ATP, a turn-off fluorescent probe was constructed, demonstrating a linear response within 1–30 μM and a detection limit of 0.105 μM. Owing to its biocompatibility and inherent mitochondrial-targeting ability, it enabled effective intracellular ATP monitoring. In HepG2 cells, fluorescence intensity showed clear dose-dependence upon treatment with ATP-activator (Glimepiride) and ATP-inhibitor (Imatinib).</div></div><div><h3>Significance and novelty</h3><div>This work not only presents a sensitive and selective probe for ATP determination but also establishes a versatile platform for real-time tracking of intracellular ATP levels and screening of ATP-modulating drugs. The proposed design strategy based on metal-coordination enhanced bimetallic NCs offers a novel pathway for developing organelle-targeted functional probes, highlighting its potential in biomedical sensing and pharmacological research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345170"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146095662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}