Analytica Chimica Acta最新文献

{"title":"Electrochemical β-lactamase immunostrip sensor with 3D hydrogel-paper scaffold for rapid detection & post-antibiotic therapy monitoring in drug-resistant bloodstream infections","authors":"Malvika Shukla ,&nbsp;Dhruvesh Maiya ,&nbsp;Rimpal Malaviya ,&nbsp;Mruga Raval ,&nbsp;Dolatsinh Zala ,&nbsp;Vaibhav Bhatt ,&nbsp;Shubhita Tripathi ,&nbsp;Alok Pandya","doi":"10.1016/j.aca.2025.343953","DOIUrl":"10.1016/j.aca.2025.343953","url":null,"abstract":"<div><h3>Background</h3><div>The increasing prevalence of drug-resistant bacterial bloodstream infections, particularly those caused by Methicillin-resistant Staphylococcus aureus (MRSA), presents a critical global healthcare challenge. Current diagnostic methods often lack the speed and sensitivity necessary for timely antibiotic interventions, leading to poor patient outcomes and increased resistance due to misuse of broad-spectrum antibiotics. Existing platforms rarely combine rapid detection, low detection limits, and real-time therapy monitoring, leaving a crucial gap in effective infection management.</div></div><div><h3>Results</h3><div>This study introduces an electrochemical immunostrip sensor for the rapid detection of β-lactamase (BL), an enzyme associated with drug resistance. Using a novel 3D hydrogel-paper scaffold, the sensor achieves a detection limit of 0.146 mU/ml and accurately detects BL-producing pathogens, including MRSA, from clinical samples with bacterial loads as low as 10² CFU/ml. The platform provides post culture detection results within 1 h, post antibiotic therapy monitoring within 4 h and demonstrates high specificity (∼100 %) by differentiating BL-producing strains from non-producing isolates.</div></div><div><h3>Significance and novelty</h3><div>This study introduces a new electrochemical smart immunostrip sensor integrated with a 3D hydrogel-paper scaffold for β-lactamase detection, which offers high sensitivity and specificity. Unlike conventional diagnostics, it enables user-friendly, rapid, cost-effective detection within 1 h post-blood culture and real-time antibiotic therapy monitoring in just 4 h, transforming clinically actionable point-of-care (POC) management of drug-resistant bloodstream infections.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343953"},"PeriodicalIF":5.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A safety and absolute activity measurement method for Phi29 DNA polymerase based on chemiluminescent detection of dATP consumption","authors":"Xuefeng Wang ,&nbsp;Ping Gui ,&nbsp;Xiao Li ,&nbsp;Feng Lu ,&nbsp;Wei Jing ,&nbsp;Changlong Li ,&nbsp;Zelin Lu ,&nbsp;Yanna Lin ,&nbsp;Huancai Yin ,&nbsp;Haichao Li ,&nbsp;Fuqiang Ma","doi":"10.1016/j.aca.2025.343952","DOIUrl":"10.1016/j.aca.2025.343952","url":null,"abstract":"<div><h3>Background</h3><div>Phi29 DNA polymerase serves as a cornerstone enzyme in molecular biology, enabling critical applications such as rolling-circle amplification, multiple strand-displacement amplification, and single-molecule real-time sequencing. Despite its widespread use, traditional methods for assessing its activity—including radioactive labeling and fluorescence-based quantification—suffer from limitations such as operational complexity, low precision, and safety risks. These challenges have hindered standardized quality control in both academic and industrial settings.</div></div><div><h3>Results</h3><div>To address these limitations, we developed a chemiluminescence-based absolute quantitation method that directly measures dATP consumption during polymerization. This method streamlines operational workflows by eliminating the need for multi-step purification procedures or specialized equipment, enabling the quantification of Phi29 DNA polymerase activity within 2 h. It demonstrates robust linearity and sensitivity across a broad dynamic range (25–200 μg/mL), while employing chemiluminescence-based detection of dATP to replace ³H-labeled dTTP, thereby eliminating biohazard risks associated with radioactive materials and enhancing feasibility for routine laboratory implementation.</div></div><div><h3>Significance</h3><div>This method introduces a novel approach for determining DNA polymerase activity by pioneering the correlation between dATP stoichiometry and enzymatic activity. It expands the applicability of activity assays to routine molecular biology laboratories, enabling rapid inter-batch consistency testing in commercial enzyme production. This advancement establishes a new benchmark for polymerase quality control.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343952"},"PeriodicalIF":5.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood detection of autoimmune encephalitis based on laser-induced breakdown spectroscopy and Raman spectroscopy","authors":"Zhifang Zhao ,&nbsp;Wangshu Xu ,&nbsp;Geer Teng ,&nbsp;Xiangjun Xu ,&nbsp;Bingheng Lu ,&nbsp;Hao Zhou ,&nbsp;Leifu Wang ,&nbsp;Yuge Liu ,&nbsp;Shuai Xu ,&nbsp;Qianqian Wang ,&nbsp;Wenping Ma","doi":"10.1016/j.aca.2025.343948","DOIUrl":"10.1016/j.aca.2025.343948","url":null,"abstract":"<div><h3>Background</h3><div>Recently, the incidence range of autoimmune encephalitis (AE) in people has rapidly expanded, and the diagnosis procedure of clinical criteria for AE remains complicated. Herein, with advantages of rapid speed, simple pre-treatment, and slightly destructive or non-destructive analysis, the feasibility of integrating laser-induced breakdown spectroscopy (LIBS) and Raman techniques to identify blood of AE patients was explored, and the mechanisms of medical diagnosis from atomic and molecular perspectives were further analyzed.</div></div><div><h3>Results</h3><div>In the experiment, etched mesh silicon wafers were used as serum substrates to reduce the spectral variability during measurements. Totally, 1785 LIBS spectra and 1785 Raman spectra were collected from 119 people (79 healthy people and 40 AE patients), respectively. Fusion spectra were formed by connecting LIBS spectra in series behind with Raman spectra. With mutual information (MI) method, 537 features were selected from fusion spectra, and the accuracy and test time of long short-term memory model using these features were 95.04 % and 0.95 s, an improvement by 14.36 %, 8.03 %, 2.22 % and 0.48 s, 0.08 s, 0.55 s compared to using LIBS spectra, Raman spectra and fusion spectra, respectively. Besides, the correlations between spectra and cytokines were analyzed by the Pearson’s correlation coefficient. Both metal atoms such as Na and K and molecules such as tryptophan, deoxyribose and phenylalanine were related to cytokines, corresponding to their MI importance in the AE diagnosis.</div></div><div><h3>Significance</h3><div>We made the first attempt to identify AE blood using fusion of spectral techniques and analyze correlation mechanism between spectra and cytokines. All results demonstrated that it is feasible to accurately identify AE serum by fusing LIBS and Raman techniques, which is expected to effectively assist the clinical diagnosis of AE in the future.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343948"},"PeriodicalIF":5.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AIE-based dual-ratiometric chemosensor for detection of sulfur dioxide derivatives and viscosity and its application in food samples and biological imaging","authors":"Na Tang ,&nbsp;Ruian Sun ,&nbsp;Kangshuo Xu ,&nbsp;Haichang Ding ,&nbsp;Yuling Zeng ,&nbsp;Yuan Ji ,&nbsp;Gang Liu ,&nbsp;Shouzhi Pu","doi":"10.1016/j.aca.2025.343949","DOIUrl":"10.1016/j.aca.2025.343949","url":null,"abstract":"<div><h3>Background</h3><div>SO₂ is an important gas molecular messenger that participates in many physiological activities and helps maintaining the redox homeostasis in biological system. Especially, SO₂ can effectively regulate oxidative stress and improve mitochondrial morphology. Abnormal mitochondrial viscosity can lead to a variety of diseases. Therefore, it is of great importance to detect SO₂ derivatives and cell viscosity in biological systems.</div></div><div><h3>Results</h3><div>Here, two mitochondria-targeted and dual-ratiometric chemosensors (<strong>TPE-1</strong> and <strong>TPE-2</strong>) based on tetraphenylethene were constructed, which could be used to simultaneously detect SO₂ derivatives and cell viscosity. Notably, <strong>TPE-2</strong> as the preferable due to its superior discernibility to HSO₃⁻ than that of <strong>TPE-1</strong>. Moreover, <strong>TPE-2</strong> featured rapid response, high sensitivity, excellent selectivity, and low cytotoxicity under physiological conditions which means that <strong>TPE-2</strong> is more suitable for practical application than <strong>TPE-1</strong>. The response mechanism of <strong>TPE-2</strong> for HSO₃⁻ was confirmed by the theoretical calculation and mass spectral analysis. Meanwhile, <strong>TPE-2</strong> showed enhanced fluorescence intensity the increased viscosity environment due to it was subjected to structural rotation. In addition, the practical application for detecting HSO₃⁻ in real water samples was successfully carried out with good recovery, the test strips loaded <strong>TPE-2</strong> was prepared and the detection of HSO₃⁻ in food samples was realized by a convenient smartphone analysis. Importantly, <strong>TPE-2</strong> had good mitochondrial targeting ability and had successfully imaged HSO₃⁻ and viscosity in HeLa cells and zebrafish.</div></div><div><h3>Significance</h3><div>This study provides an effective and potential tool for detecting HSO₃⁻ and viscosity in mitochondria and in vivo, the rational design strategy in this work holds potential to inspire the development of more powerful dual-response chemosensor for other biological factors.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343949"},"PeriodicalIF":5.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gas-liquid chromatography as a tool to determine vapor pressure of low-volatility pesticides: a critical study","authors":"Clara Parzanese, Carlina Lancioni, Cecilia Castells","doi":"10.1016/j.aca.2025.343932","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343932","url":null,"abstract":"<h3>Background</h3>The vapor pressure of pesticides at room temperature is one of the fundamental properties for predicting their volatilities and the likelihood of their movement into the environment. It is also highly valuable for decision making in agriculture and agro-industries. Direct methods applicable to low-volatile substances include gas saturation and molecular effusion techniques; however, they have several drawbacks. We propose an indirect method based on gas-liquid chromatography using short capillary columns coated with stationary phases with high phase ratio and operated at very high flow rates.<h3>Results</h3>The method was applied to determine the vapor pressure of twenty pesticides, ranging from intermediate to very low volatilities (&lt;0.133 mPa). Chromatographic assumptions were critically and comprehensively discussed, and the precision and accuracy of the method were evaluated using three fatty acid methyl esters (referred as validation solutes) with known and reliable vapor pressures available over a wide range of temperatures. The relative standard deviations of the primary data were below 4% and increased to 18% for extrapolated vapor pressures. The measurement conditions allowed for the extension of retention measurements to relatively lower temperatures while ensuring reasonable operational times, thereby, minimizing the extent of temperature extrapolation required. The target pesticides included widely used chemical compounds with multiple functional groups and molecules exhibiting configurational isomerism, whose individual vapor pressure could be distinguished provided that sufficient chromatographic selectivity was achieved.<h3>Significance</h3>This work provides a readily usable technique which employs instruments commonly found in both academic and industrial laboratories. Furthermore, the method is simple, robust, and reliable, allowing to estimate the vapor pressures of low-volatile and semi-volatile pesticides within reasonable analysis time and overcoming the main limitations associated with traditional methods.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"42 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pixel array-based urine biosensor for detection of trimethylamine N-oxide and glucose for early detection of diabetic kidney disease","authors":"Mei-Ching Yu ,&nbsp;Chi-Jen Lo ,&nbsp;Wei-Cheng Lin ,&nbsp;Wei-Lun Yen ,&nbsp;Yun-Yu Hsieh ,&nbsp;Bing-Hong Chen ,&nbsp;Fu-Sung Lo","doi":"10.1016/j.aca.2025.343951","DOIUrl":"10.1016/j.aca.2025.343951","url":null,"abstract":"<div><div>Trimethylamine N-oxide (TMAO) serves as a crucial biomarker for early detection and prevention of cardiovascular and chronic kidney diseases. In this study, we design and implement a novel pixel array-based urine biosensor to explore the relationship between TMAO levels and glucose in urine and the urine albumin-creatinine ratio (UACR). The urine biosensor, incorporating a specialized readout circuit, measures TMAO across various UACR ranges, revealing a linear correlation with a slope of 8.5 mV per mg/g up to 1100 mg/g UACR. Although glucose levels also rise with UACR, significant discrepancies occur beyond 30 mg/g, indicating that glucose does not consistently correlate with UACR increases. The biosensor demonstrates a sensitivity of 41 ADC counts/μM (4.5 mV/μM), a 10-s response time, 98 % reproducibility, and a drift of 0.3 mV over extended periods. It requires only 5 μL of urine for a comprehensive analysis of TMAO and glucose. This approach significantly improves time efficiency, offering a faster and more convenient solution for monitoring the risk for chronic kidney disease, such as those with diabetes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343951"},"PeriodicalIF":5.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of ultra-miniaturized weak affinity chromatography coupled to mass spectrometry as a high throughput fragment screening method against wild-type and purified membrane proteins embedded in biomimetic membranes","authors":"François-Xavier Vidal ,&nbsp;Julie Gil ,&nbsp;Maud Gregson ,&nbsp;Gabrielle Zeder-Lutz ,&nbsp;Maria Hideux ,&nbsp;Jérôme Lemoine ,&nbsp;Isabelle Krimm ,&nbsp;Renaud Wagner ,&nbsp;Vincent Dugas ,&nbsp;Claire Demesmay","doi":"10.1016/j.aca.2025.343950","DOIUrl":"10.1016/j.aca.2025.343950","url":null,"abstract":"<div><h3>Background</h3><div>Membrane proteins, which make up approximately 30 % of the proteome, are important drug targets but present many challenges in drug discovery, including limited production rates, low final yields of pure and functionally folded proteins, and instability in aqueous media. The problems encountered with membrane proteins are even more critical in the Fragment Based Drug Discovery, where the discovery of potential drug candidates is hampered by the limited availability of efficient methods for rapid screening of weak fragment-protein interactions.</div></div><div><h3>Results</h3><div>In this work, we propose the coupling of miniaturized weak affinity chromatography with mass spectrometry (nano-WAC-MS) as an innovative strategy for the rapid screening of fragments capable of weak binding to a selected membrane protein. An integral membrane protein (AA_2AR) was incorporated into biotinylated nanodiscs, which were subsequently immobilized on a miniaturized monolithic streptavidin column (75 μm i.d., 300 nL volume). The coupling of these miniaturized affinity columns (each consuming less than 1 μg of protein) to mass spectrometry (MRM mode) has been optimized to maximize the low affinity range and increase throughput so that 150 fragments can be injected in a single analysis, with a DMSO content as high as 10 %, with no influence on the affinity. Hits are identified by comparing their retention with that measured on control columns prepared with empty nanodiscs.</div></div><div><h3>Significance</h3><div>The results of this screening are compared with those obtained by NMR and newly identified hits are confirmed by either competition experiments or frontal affinity experiments. We show that this nanodisc-based strategy, which provides a stable and native-like lipid environment for the protein (columns can be used for several days), should also work with other membrane proteins embedded in nanodiscs.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343950"},"PeriodicalIF":5.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collinear datasets augmentation using Procrustes validation sets","authors":"Sergey Kucheryavskiy ,&nbsp;Sergei Zhilin","doi":"10.1016/j.aca.2025.343913","DOIUrl":"10.1016/j.aca.2025.343913","url":null,"abstract":"<div><h3>Background:</h3><div>high complexity models, such as artificial neural networks (ANN), require large datasets for training to avoid overfitting and reproducibility issues. However, experimental datasets, especially those involving spectroscopic or other highly collinear data, often suffer from limited size due to practical constraints. Currently available data augmentation methods, either do not handle collinearity well, or require resource-intensive training. Thus, there is a pressing need for an efficient, scalable method for augmenting collinear datasets to enhance model performance in both regression and classification tasks.</div></div><div><h3>Results:</h3><div>we propose a novel, efficient data augmentation method tailored for datasets with moderate to high collinearity, particularly spectroscopic data. This method utilizes latent variable modeling combined with cross-validation resampling to generate new data points. The approach has been validated using varios datasets, here we report detailed results for two case studies: fat content prediction in minced meat and discrimination of olives based on near-infrared spectra. In both cases, artificial neural networks were employed, resulting in significant improvements in model performance in prediction and classification. Specifically, for fat content prediction, the method reduced the root mean squared error by up to 3-fold on the independent test set.</div></div><div><h3>Significance</h3><div>: the proposed method provides a fast and simple solution for augmenting collinear datasets, significantly improving model performance without requiring extensive parameter tuning. It is versatile and can be applied to a range of datasets, offering a practical alternative to more complex augmentation techniques.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343913"},"PeriodicalIF":5.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of lipid nanoparticles using macro mass photometry: Insights into size and mass","authors":"Jikang Wu ,&nbsp;Yu Zhang ,&nbsp;Zhijie Wu ,&nbsp;Julia Townsend ,&nbsp;Ivor Crooks ,&nbsp;Brenda Watt ,&nbsp;Andrew Baik ,&nbsp;Katherine Cygnar ,&nbsp;Haibo Qiu ,&nbsp;Ning Li","doi":"10.1016/j.aca.2025.343944","DOIUrl":"10.1016/j.aca.2025.343944","url":null,"abstract":"<div><h3>Background</h3><div>Lipid nanoparticles (LNPs) have become an important delivery system for nucleic acids, as applied in the first RNAi drug and two COVID-19 mRNA vaccines approved by the FDA. Despite advantages of their high cargo capacity, low immunogenicity allowing for redosing, scalability and low-cost manufacturing, challenges such as liver accumulation and difficulties in quality control persist for LNPs development. Conjugation of antibodies or antibody fragments onto LNPs holds promise in achieving precise targeting and higher stability of the targeting moieties on LNP surfaces. However, quality control of such multiple-component products poses additional challenges compared to LNP alone. LNP size and mass are critical quality attributes which play important roles in determining LNPs’ nucleic acid cargo loading, antibody conjugation level, biodistribution, targeting capability, and overall efficacy.</div></div><div><h3>Results</h3><div>Macro mass photometry (MMP), a single-molecule technique, enabled orthogonal characterization of LNPs by incorporating contrast analysis as a proxy for particle mass and combining it with size measurement. This approach thus improves the definition of LNP species within heterogeneous systems. Using MMP, this study revealed the effects of PEG-lipid concentration, mRNA encapsulation, and antibody conjugation on LNP size and mass. Additionally, the study demonstrated the MMP's utility in monitoring LNPs under various stress conditions, including high pH, low pH, oxidation, freeze-thaw cycles, and agitation.</div></div><div><h3>Significance</h3><div>This study represents the first use of mass photometry for LNP analysis, simultaneously characterizing the mass and size of LNPs. It highlights the potential of MMP as a valuable orthogonal tool for LNP characterization and supports LNP formulation development.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343944"},"PeriodicalIF":5.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surfactant-based coatings for protein separation by capillary electrophoresis - A review","authors":"Chirapha Prakobdi, Laura Dhellemmes, Laurent Leclercq, Gaulthier Rydzek, Hervé Cottet","doi":"10.1016/j.aca.2025.343945","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343945","url":null,"abstract":"<h3>Background</h3>Capillary electrophoresis (CE) is a highly efficient and versatile analytical method for the separation of biomacromolecules such as proteins and peptides. One major concern to reach high separation efficiency is the adsorption of analytes on the capillary wall and the heterogeneity of the capillary surface charge which generates hydrodynamic dispersion due to local electroosmotic (EOF) fluctuations.<h3>Results</h3>Double chain surfactants have been described as potential interesting candidates for capillary coatings in CE. They are notably offering a very homogenous surface charge leading to very high separation efficiency with reported values up to 1 million plates per meter.<h3>Significance</h3>This review provides an overview of double chain surfactant coatings used in CE with an emphasis on the coating protocol, the nature of the surfactant, the preparation of the coating solution (concentration, temperature, sonication or extrusion), the physicochemical parameters affecting their properties (pH, ionic strength, nature of the anion in the coating solution, coating additives, capillary internal diameter), and the coating stability / durability.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"183 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}