Analytica Chimica Acta最新文献

{"title":"Electrochemistry–glucosemeter–smartphone integrated multi-mode biosensor for accurate detection of aflatoxin B1","authors":"Zhiguang Suo ,&nbsp;Tao Lu ,&nbsp;Jiahui Liu ,&nbsp;Jiayi Li ,&nbsp;Li Wang ,&nbsp;Yong Liu ,&nbsp;Min Wei","doi":"10.1016/j.aca.2024.343398","DOIUrl":"10.1016/j.aca.2024.343398","url":null,"abstract":"<div><h3>Background</h3><div>Aflatoxin B1 (AFB1) is a widely distributed toxic contaminant in food and poses a serious threat to public health. Therefore, an accurate, simple, cost-effective and on-site assay method is needed for sensitive detection of AFB1. Aptamer shows great potential in the construction of biosensor due to its high specificity and affinity. Multimodal biosensor based on aptamer is highly suitable for the analysis of AFB1 under complex conditions. And the detection results in different modes can be verified with each other, which greatly improves the accuracy of AFB1 detection.</div></div><div><h3>Results</h3><div>Herein, accurate detection of AFB1 was achieved through the development of a multi-mode biosensor integrating electrochemistry, glucosemeter and smartphone-based colorimetric quantification. Streptavidin-Cu₃(PO₄)₂ hybrid nanoflowers (SA-Cu₃(PO₄)₂ HNFs) were synthesised and then conjugated with biotinylated invertase as a signal probe. The electrochemical signal was achieved via intrinsic redox activity. Simultaneously, sucrose could be converted to glucose by the action of invertase, which can cause changes in the glucosemeter signal as well as in the colour of urine glucose test strips. The glucosemeter could complete the signal response in 7 s, and the urine glucose test strips could complete the colour development in 30 s. The detection range of AFB1 by this system in electrochemical mode is 0.001–100 ng/mL, and in glucosemeter mode and smartphone mode is 0.01–50 ng/mL. The limits of detection were 0.49 pg/mL in electrochemistry mode, 5.4 pg/mL in glucosemeter mode and 3.7 pg/mL in smartphone mode.</div></div><div><h3>Significance</h3><div>The successful construction of this multi-mode biosensor demonstrates the advantages of multifunctional nanomaterials and mobile technology. Rapid and accurate detection of AFB1 is achieved through the integration of electrochemistry, glucosemeter and smartphone-based colorimetric quantification. And this biosensor provides a novel detection platform that combines sensitivity, accuracy, affordability and portability for rapid on-site food safety screening.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343398"},"PeriodicalIF":5.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of C-reactive protein using a label-free NIR fluorescent aptasensor with a large Stokes shift based on an AIEE anthracene derivative","authors":"Zhuowei Fu ,&nbsp;Yiwen Yang ,&nbsp;Zhifeng Li ,&nbsp;Yanbo Zeng ,&nbsp;Hailong Wang ,&nbsp;Yifeng Han ,&nbsp;Qiukai Tang ,&nbsp;Lei Li","doi":"10.1016/j.aca.2024.343386","DOIUrl":"10.1016/j.aca.2024.343386","url":null,"abstract":"<div><h3>Background</h3><div>C-reactive protein (CRP), one of the classic biomarkers of inflammation, is closely related to infectious inflammation, cardiovascular disease, cancer, and other diseases. Therefore, timely and accurate detection of CRP in human blood is crucial for the discovery, diagnosis, and treatment of the aforementioned diseases. Herein, a novel label-free NIR fluorescence aptasensor with a large Stokes shift based on an AIEE anthracene derivative B and a molybdenum disulfide (MoS₂) platform was developed and used for the high sensitivity and specificity detection of CRP.</div></div><div><h3>Results</h3><div>Compound B could emit near-infrared (NIR) fluorescence with a large Stokes shift (190 nm). Notably, this compound could bind with the aptamer of CRP (CRP-Apt) through electrostatic attraction to form a B/CRP-Apt complex, generating an aggregation-induced emission enhancement effect and enhancing the fluorescent intensity of B. B/CRP-Apt could be adsorbed on the surface of MoS₂ with the addition of MoS₂ to its solution, and the fluorescence of Compound B was quenched. CRP was then added to the above solution. CRP-Apt had a substantially higher affinity for CRP than MoS₂. Therefore, B/CRP-Apt detached from the surface of MoS₂ and bound to CRP, thereby restoring the fluorescence of B. Experimental results showed a good linear relationship between the fluorescent recovery intensity of B and the concentration of CRP in the concentration range of 0.3–70 ng mL⁻¹, with a limit of detection as low as 0.1 ng mL⁻¹.</div></div><div><h3>Significance and novelty</h3><div>The aptasensor integrates the advantages of high sensitivity of NIR fluorescence, high specificity of aptamers, good water-solubility and AIEE effect of Compound B. And it could be applied to the determination of CRP in human serum samples, while most of the reported methods can only determine CRP in spiked human serum samples.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343386"},"PeriodicalIF":5.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raw data and noise in spectrophotometry","authors":"Bruna Falgueras Vallbona ,&nbsp;Ardiana Kajtazi ,&nbsp;Golnaz Shahtahmassebi ,&nbsp;Quentin S. Hanley","doi":"10.1016/j.aca.2024.343393","DOIUrl":"10.1016/j.aca.2024.343393","url":null,"abstract":"<div><h3>Background</h3><div>Spectrophotometers are ubiquitous in chemical and biological science; however, their precision limits are under-appreciated. Rules-of-thumb and IUPAC referenced guidance restricting absorbance ranges to minimize uncertainty are based on historically important instruments which are no longer as widely used. Advances over the last half-century changed most “raw” data from absorbance and transmittance values directly produced in analog electronics to digitized intensities. The latter are rarely provided in favour of digitally transformed derived data. Assessment of spectrophotometer limitations using digitized intensities would be straightforward with mean-variance analysis. However, in their absence, derived data evaluated at scale allows efficient characterisation of modern spectrophotometers.</div></div><div><h3>Results</h3><div>This study analyses signals when <em>I</em> and <em>I</em>_<em>0</em> are not available and only absorbance or transmittance are obtained. Current IUPAC referenced guidance indicates that absorbance should be limited between 0.1 and 1.0 a.u. with optimal performance (minimum relative standard deviation (RSD)) at 0.43 a.u. or 0.86 a.u. depending on the type of limiting noise. We characterised noise in UV–Vis spectrophotometers using three methods and report optimality spectra for the first time. We found the instruments were not Poisson optimal and best RSDs were sometimes above 1.0 a.u. We could find no evidence justifying guidance restricting absorbance to between 0.1 and 1.0 a.u. Measured RSD and light intensity are more important than absorbance values for assuring good quality measurements. However, estimating light intensity is a difficult inverse problem when <em>I</em> and <em>I</em>₀ are not available, and the tested commercial instruments did not provide these.</div></div><div><h3>Significance</h3><div>Based on this work, classical theories are insufficient to describe spectrophotometers accurately. Furthermore, we urge IUPAC to modernise the references in its Gold Book and press instrument makers to improve data transparency. These steps are crucial to use spectrophotometers optimally.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343393"},"PeriodicalIF":5.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High sensitive and discriminating colorimetric assay for S2− overload in adaptogenic herbs utilizing ZnS nanoparticle-enhanced S, N-doped eggshell membrane-derived biochar","authors":"Dapeng Xu ,&nbsp;Yanning Zheng ,&nbsp;Bimei Xie ,&nbsp;Youquan Zhang ,&nbsp;Zhangfa Tong ,&nbsp;Jianhua Sun ,&nbsp;Lixia Sun ,&nbsp;Guangzhi Zhou ,&nbsp;Dankui Liao","doi":"10.1016/j.aca.2024.343391","DOIUrl":"10.1016/j.aca.2024.343391","url":null,"abstract":"<div><h3>Background</h3><div>With the rapid development of industrialization, the excessive emission of S²⁻ have become increasingly serious, leading to a surge in the content of S²⁻ in nature. Rapid and accurate detection of S²⁻ contamination in natural adaptogens is crucial for food safety. Annually, discarded eggshell waste, rich in organic and inorganic materials, poses environmental risks if landfilled. Utilizing waste eggshell membrane biomass for S²⁻ detection is cost-effective, yet designing biochar materials for sensors requires balancing catalytic enhancement and anti-interference capabilities. Improving the catalytic performance of biochar for colorimetric S²⁻ detection without metal ion interference presents a challenging issue.</div></div><div><h3>Results</h3><div>We first modified biochar (EBc) derived from waste eggshell membranes using a combination of thiourea and ZnS nanoparticles, fabricating ZnS-decorated, S–N co-doped biochar (ZnS-SN-EBc) nanozymes, which were applied for the colorimetric assay detection of S²⁻ contamination. The addition of thiourea significantly increases the proportion of pyridinic-N in biochar, enhancing the peroxidase-like activity of the nanozyme. The growth of ZnS nanoparticles on the biochar not only enhances the catalytic performance by increasing the S content but also reduces the content of oxidized S, thereby improving resistance to interference. The detection range for S²⁻ was expanded from 0.1 to 45 μM for EBc to 0.05–225 μM for ZnS-SN-EBc, and the limit of detection improved to 0.0397 μM. Additionally, ZnS-SN-EBc significantly enhanced metal ion interference resistance. S²⁻ detection in five types of adaptogenic herbs verified the accuracy and practicality of the colorimetric assay, with recovery rates comparable to national standards.</div></div><div><h3>Significance</h3><div>We innovatively repurposed waste eggshell membranes to develop a selective and catalytic peroxidase-like nanozyme, ZnS-decorated S–N co-doped biochar (ZnS-SN-EBc). The developed colorimetric assay utilizing ZnS-SN-EBc demonstrates significant potential for the detection of sulfur ions in adaptogenic herbs, thus contributing to both waste resource utilization and the advancement of food safety detection technologies.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343391"},"PeriodicalIF":5.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A portable and sensitive DNA-based electrochemical sensor for detecting piconewton-scale cellular forces","authors":"Mahmoud Amouzadeh Tabrizi ,&nbsp;Ahsan Ausaf Ali ,&nbsp;Murali Mohana Rao Singuru ,&nbsp;Lan Mi ,&nbsp;Priyanka Bhattacharyya ,&nbsp;Mingxu You","doi":"10.1016/j.aca.2024.343392","DOIUrl":"10.1016/j.aca.2024.343392","url":null,"abstract":"<div><h3>Background</h3><div>Cell-generated forces are a key player in cell biology, especially during cellular shape formation, migration, cancer development, and immune response. The measurement of forces exerted and experienced by cells is fundamental in understanding these mechanosensitive cellular behaviors. While cell-generated forces can now be detected based on techniques like fluorescence microscopy, atomic force microscopy, optical/magnetic tweezers, however, most of these approaches rely on complicated instruments or materials, as well as skilled operators, which could limit their potential broad applications in regular biological laboratories.</div></div><div><h3>Results</h3><div>A new type of smartphone-based electrochemical sensor is developed here for cellular force measurement. In this system, a double-stranded DNA-based force probe, known as tension gauge tether, is attached to the surface of a gold screen-printed electrode, which is then incorporated into a portable smartphone-based electrochemical device. Cellular force-induced DNA detachment on the sensor surface results in multiple redox reporters to reach the surface of the electrode and generate enhanced electrochemical signals. To further improve the sensitivity, a CRISPR-Cas12a system has also been incorporated to cleave the remaining surface-attached anchor DNA strand. Using integrin-mediated tension as an example, piconewton-scale adhesion forces generated by ≤ 10 HeLa cells could now be reliably detected. Meanwhile, the threshold forces of these electrochemical sensors can also be modularly tuned to detect different levels of cellular forces.</div></div><div><h3>Significance</h3><div>These novel DNA-based highly sensitive, portable, cost-efficient, and easy-to-use electrochemical sensors can be potentially powerful tools for detecting different cell-generated molecular forces. Functioning as complementary tools with traction force microscopy and fluorescent probes, these electrochemical sensors can be straightforwardly applied in regular biological laboratories for understanding the basic mechanical principles of cell signaling and for developing novel strategies and materials in tissue engineering, regenerative medicine, and cell therapy.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343392"},"PeriodicalIF":5.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel blood spot with oil sealing for antioxidation and completeness preservation of erythrocytes and hemoglobin","authors":"Genhan Zha ,&nbsp;Xuan Xiao ,&nbsp;Youli Tian ,&nbsp;Hengying Zhu ,&nbsp;Qiang Zhang ,&nbsp;Changjie Yu ,&nbsp;Honggen Li ,&nbsp;Yuxing Wang ,&nbsp;Chengxi Cao ,&nbsp;Ping Chen","doi":"10.1016/j.aca.2024.343390","DOIUrl":"10.1016/j.aca.2024.343390","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343390"},"PeriodicalIF":5.7,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bottom-up designing nanostructured oxide libraries under a lab-on-chip paradigm towards a low-cost highly-selective E-nose","authors":"Maksim A. Solomatin ,&nbsp;Fedor S. Fedorov ,&nbsp;Demid A. Kirilenko ,&nbsp;Vanessa Trouillet ,&nbsp;Alexey S. Varezhnikov ,&nbsp;Ilia V. Kiselev ,&nbsp;Udo Geckle ,&nbsp;Martin Sommer ,&nbsp;Alexey M. Bainyashev ,&nbsp;Vasily Artemov ,&nbsp;Nikolay M. Ushakov ,&nbsp;Vladimir G. Goffman ,&nbsp;Maxim K. Rabchinskii ,&nbsp;Albert G. Nasibulin ,&nbsp;Victor V. Sysoev","doi":"10.1016/j.aca.2024.343387","DOIUrl":"10.1016/j.aca.2024.343387","url":null,"abstract":"<div><h3>Background</h3><div>The multisensor concept has been developed as a powerful alternative to well-known gas-analytical instrumentation for applications where a fast but accurate and reliable assessment of the environment is required. The concept follows a biology-inspired approach where the selectivity towards various gases/odors is attained <em>via</em> pattern recognition of multisensory signal vectors. Herein, we discuss how to design a selective multisensor library based on various metal oxide nanostructures like a lab-on-chip using a simple but efficient bottom-up growth of materials over the multi-electrode chip under robust dc electrochemical protocols.</div></div><div><h3>Results</h3><div>In addition to a conventional growth of oxide layers over the metal electrodes, we show that the fine nanowall-like oxide structures appear as a quasi-matrixed percolation film over the SiO₂ substrate surface in the inter-electrode gaps to constitute a chemiresistive film. We have tested two directions while applying the technique to grow Co, Ni, Mn, and Zn oxides to develop on-chip sensor arrays of, (i) monoxide type employing the oxide films with gradual change of growth time, and (ii) multi-oxide type based on the four oxides. The materials were thoroughly characterized by electron microscopy, X-ray diffraction, thermogravimetric analysis, and X-ray photoelectron spectroscopy/mapping to prove the composition and structure. Among tested oxides, ZnO readily appears not only at the electric potential-targeted chip zone but also in other areas to dope the films for yielding heterojunctions with other oxides that enhances a variability of functional properties in the on-chip sensor array. The gas-sensing performance of the chips has been tested <em>versus</em> various chemically <em>akin</em> alcohol vapors at the sub- and low ppm range of concentrations in a mixture with air.</div></div><div><h3>Significance</h3><div>We show that the grown oxide nanostructures exhibit a high-sensitive chemiresistive signal which allows one to build a multisensor vector signal, selective to the kind of alcohols, even at sub-ppm concentrations. Moreover, the multi-oxide library yields options for a superior selectivity under LDA metrics than the gradient-grown mono-oxide one due to the versatility of materials while the low-cost growth protocols remain to be the same in both cases. The delivered method to produce multisensor arrays allows one producing low-cost but efficient electronic nose units for numerous applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343387"},"PeriodicalIF":5.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering food analysis: Transformative antioxidant testing with colorimetric assays using Co–Mn bimetallic oxide nanozyme","authors":"Shuyun Chen,&nbsp;Zhichao Yu,&nbsp;Yunsen Wang,&nbsp;Shuo Tian,&nbsp;Qiaohua Wei,&nbsp;Dianping Tang","doi":"10.1016/j.aca.2024.343389","DOIUrl":"10.1016/j.aca.2024.343389","url":null,"abstract":"<div><h3>Background</h3><div>Total antioxidant capacity (TAC) testing is critical for guiding dietary, and nutritional treatments, and investigating the efficacy of antioxidant food additives.</div></div><div><h3>Results</h3><div>Here, a competitive enzyme-like colorimetric method based on Co–Mn nanoflower nanozymes was developed for the determination of TAC in food. The synthesized nanozymes had excellent POD-like activity, using the antioxidant as a special recognition unit and the chromogenic substrate as a signal molecule reporter, and could generate a significant color response in less than 15 min by producing enzymatically catalyzed ROS. Antioxidants in food were introduced into the system and inhibited the oxidative attack of reducing substances, which was signaled through signal molecules. The established TAC sensor had a sensitivity response of 1–20 μg/mL and a low detection limit of 0.1 μg/mL. It was worth noting that the developed detection strategy not only meets the criterion of a reduced elapsed time for the HPLC method but also achieves high-throughput batch detection. The examination of each component can be accomplished in 10–20 s while maintaining the same precision as the commercially available HPLC approach.</div></div><div><h3>Significance</h3><div>The model designed based on simulated enzyme materials is a successful exploration of the application of nanozymes in the field of food quality testing, which will further realize the accurate and comprehensive analysis of TAC in the future.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343389"},"PeriodicalIF":5.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared SPR biosensor based on photonic crystal fiber for DNA hybridization detection","authors":"Zhiyong Yin ,&nbsp;Zhibing Zhang ,&nbsp;Xili Jing,&nbsp;Linchuan Hu,&nbsp;Shuguang Li,&nbsp;Jianshe Li","doi":"10.1016/j.aca.2024.343385","DOIUrl":"10.1016/j.aca.2024.343385","url":null,"abstract":"<div><h3>Background</h3><div>Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS₂ to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on.</div></div><div><h3>Results</h3><div>The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS₂ as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L).</div></div><div><h3>Significance</h3><div>The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1333 ","pages":"Article 343385"},"PeriodicalIF":5.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advantages of dimethyl carbonate as organic modifier for enantioseparation of novel psychoactive substances in sub/supercritical fluid chromatography","authors":"Denisa Folprechtová ,&nbsp;Elisabeth Seibert ,&nbsp;Martin G. Schmid ,&nbsp;Květa Kalíková","doi":"10.1016/j.aca.2024.343380","DOIUrl":"10.1016/j.aca.2024.343380","url":null,"abstract":"<div><h3>Background</h3><div>Sub/supercritical fluid chromatography is regarded as a greener separation technique due to the use of carbon dioxide as the main component of the mobile phase compared to conventional liquid chromatography techniques. Organic co-solvents are usually added to carbon dioxide to increase elution strength of the mobile phase. Therefore, it is of great importance to test applicability of green co-solvents in separation methods and to include them among commonly used mobile phase components.</div></div><div><h3>Results</h3><div>A comprehensive study of the suitability of green solvent dimethyl carbonate as a co-solvent for enantioseparation in sub/supercritical fluid chromatography was conducted with a set of novel psychoactive substances from various groups. The experiments were performed on polysaccharide-based columns. For successful enantioseparation of these compounds, the presence of basic or mixed mobile phase additives was essential. The obtained results clearly show that dimethyl carbonate is a suitable co-solvent for enantioseparation on polysaccharide-based columns in sub/supercritical fluid chromatography and in some cases surpasses commonly used co-solvents as methanol and propan-2-ol.</div></div><div><h3>Significance</h3><div>The use of more sustainable co-solvents, such as dimethyl carbonate, instead of conventional ones to carbon dioxide presents a greener approach to analytical applications and reduces the overall environmental impact of analytical processes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343380"},"PeriodicalIF":5.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}