Pub Date : 2026-03-24DOI: 10.1016/j.aca.2026.345436
Jiaqin Chen, Guangxu Hu, Jie Song, Lin Huang, Xumin Zhang
Deciphering protein-ligand binding events is essential for unraveling biological pathways and advancing drug development. The recently developed peptide-centric local stability assay (PELSA) has demonstrated superior performance compared to other mainstream approaches. However, the conventional PELSA approach relies on filter-based peptide recovery, which results in prolonged processing times and relatively low yields, particularly when operating with limited starting material. To address these limitations, we present an optimized PELSA approach termed SP3-PELSA, which leverages the simple and efficient peptide recovery of the Single-Pot, Solid-Phase-enhanced Sample Preparation (SP3) method. Through a systematic evaluation of protein precipitation and trypsin inactivation conditions, we identified 1% formic acid and 80% acetonitrile as the optimal parameters. A comparative analysis of SP3-PELSA with conventional PELSA revealed that SP3-PELSA reduces the processing time by fivefold and significantly increases the peptide recovery rate, especially for hydrophobic peptides. Consequently, SP3-PELSA outperformed other mainstream approaches in the identification of established drug targets. Owing to its high recovery efficiency and potential for automation, SP3-PELSA holds great promise for large-scale drug target discovery, especially in the context of scarce clinical samples. Furthermore, its exceptional recovery of hydrophobic peptides positions this approach as a powerful tool for analyzing highly hydrophobic proteins in drug target screening.
{"title":"SP3-PELSA: A Rapid and Sensitive Approach for Studying Protein-Ligand Interaction with Small-Amount Samples","authors":"Jiaqin Chen, Guangxu Hu, Jie Song, Lin Huang, Xumin Zhang","doi":"10.1016/j.aca.2026.345436","DOIUrl":"https://doi.org/10.1016/j.aca.2026.345436","url":null,"abstract":"Deciphering protein-ligand binding events is essential for unraveling biological pathways and advancing drug development. The recently developed peptide-centric local stability assay (PELSA) has demonstrated superior performance compared to other mainstream approaches. However, the conventional PELSA approach relies on filter-based peptide recovery, which results in prolonged processing times and relatively low yields, particularly when operating with limited starting material. To address these limitations, we present an optimized PELSA approach termed SP3-PELSA, which leverages the simple and efficient peptide recovery of the Single-Pot, Solid-Phase-enhanced Sample Preparation (SP3) method. Through a systematic evaluation of protein precipitation and trypsin inactivation conditions, we identified 1% formic acid and 80% acetonitrile as the optimal parameters. A comparative analysis of SP3-PELSA with conventional PELSA revealed that SP3-PELSA reduces the processing time by fivefold and significantly increases the peptide recovery rate, especially for hydrophobic peptides. Consequently, SP3-PELSA outperformed other mainstream approaches in the identification of established drug targets. Owing to its high recovery efficiency and potential for automation, SP3-PELSA holds great promise for large-scale drug target discovery, especially in the context of scarce clinical samples. Furthermore, its exceptional recovery of hydrophobic peptides positions this approach as a powerful tool for analyzing highly hydrophobic proteins in drug target screening.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"86 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147506959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-24DOI: 10.1016/j.aca.2026.345418
R. Karan, M.K. Prasannakumar, Harvinder Kour Khera, J. Harish, Swathi.S. Patil, Pramesh Devanna, C. Manjunatha, Rakesh Kumar Mishra
Background
Rice blast, caused by Magnaporthe oryzae, is one of the most devastating fungal pathogens of rice. Early, rapid and accurate detection is critical for effective disease management and the prevention of outbreaks. Conventional and isothermal molecular methods vary widely in sensitivity and field applicability, making it difficult to understand the reliable detection. This study aimed to perform the first unified, side-by-side comparison of six molecular detection techniques using a common primer set to identify the most sensitive, specific and field deployable approach for M. oryzae detection.
Results
In this study, we have evaluated six diagnostic methods such as PCR, qPCR, LAMP, RPA, and CRISPR-Cas12a integrated with LAMP or RPA targeting the multi copy Pot2 transposon in M. oryzae. Sensitivity assays using 10-fold genomic DNA dilutions (109 to 10-1 copies/reaction) revealed that LAMP-CRISPR and RPA-CRISPR were most sensitive, detecting down to 1 copy/reaction. LAMP and qPCR detected down to 102 and 103 copies/reaction, while RPA and PCR were limited to 105 and 106 copies/reaction, respectively. All assays showed high specificity with no cross-reactivity to 10 non-target rice fungal pathogens. Field validation on 17 symptomatic samples confirmed that CRISPR-based methods outperformed traditional techniques, detecting positives missed by other platforms. This is the first systematic comparison applying the same primer set across multiple diagnostic methods for M. oryzae.
Significance
Our findings demonstrate that CRISPR-Cas12a-based platforms combined with isothermal amplification offers high sensitivity and specificity. CRISPR based detection allows strong field applicability for M. oryzae detection. The use of a unified primer set allowed for a direct performance comparison. These results highlight the potential of CRISPR-based diagnostics to enhance early pathogen detection, enabling rapid interventions and improved management of rice blast disease.
{"title":"Unified Primer Enabled Detection (UPED) system for Magnaporthe oryzae infecting rice: A comparative study from conventional PCR to CRISPR Cas12a based detection systems.","authors":"R. Karan, M.K. Prasannakumar, Harvinder Kour Khera, J. Harish, Swathi.S. Patil, Pramesh Devanna, C. Manjunatha, Rakesh Kumar Mishra","doi":"10.1016/j.aca.2026.345418","DOIUrl":"https://doi.org/10.1016/j.aca.2026.345418","url":null,"abstract":"<h3>Background</h3>Rice blast, caused by <em>Magnaporthe oryzae</em>, is one of the most devastating fungal pathogens of rice. Early, rapid and accurate detection is critical for effective disease management and the prevention of outbreaks. Conventional and isothermal molecular methods vary widely in sensitivity and field applicability, making it difficult to understand the reliable detection. This study aimed to perform the first unified, side-by-side comparison of six molecular detection techniques using a common primer set to identify the most sensitive, specific and field deployable approach for <em>M. oryzae</em> detection.<h3>Results</h3>In this study, we have evaluated six diagnostic methods such as PCR, qPCR, LAMP, RPA, and CRISPR-Cas12a integrated with LAMP or RPA targeting the multi copy <em>Pot2</em> transposon in <em>M. oryzae</em>. Sensitivity assays using 10-fold genomic DNA dilutions (10<sup>9</sup> to 10<sup>-1</sup> copies/reaction) revealed that LAMP-CRISPR and RPA-CRISPR were most sensitive, detecting down to 1 copy/reaction. LAMP and qPCR detected down to 10<sup>2</sup> and 10<sup>3</sup> copies/reaction, while RPA and PCR were limited to 10<sup>5</sup> and 10<sup>6</sup> copies/reaction, respectively. All assays showed high specificity with no cross-reactivity to 10 non-target rice fungal pathogens. Field validation on 17 symptomatic samples confirmed that CRISPR-based methods outperformed traditional techniques, detecting positives missed by other platforms. This is the first systematic comparison applying the same primer set across multiple diagnostic methods for <em>M. oryzae</em>.<h3>Significance</h3>Our findings demonstrate that CRISPR-Cas12a-based platforms combined with isothermal amplification offers high sensitivity and specificity. CRISPR based detection allows strong field applicability for <em>M. oryzae</em> detection. The use of a unified primer set allowed for a direct performance comparison. These results highlight the potential of CRISPR-based diagnostics to enhance early pathogen detection, enabling rapid interventions and improved management of rice blast disease.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"93 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147506966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-22Epub Date: 2026-01-29DOI: 10.1016/j.aca.2026.345144
Ruxuan Mo , Chao Kang , Jianping Yuan , Carl Redshaw , Ying Huang , Qilong Zhang , Xiufang Yan
Background
Hydrogen sulfide (H2S) is a key endogenous gasotransmitter involved in plant physiological regulation and stress responses. Monitoring its dynamic changes in plants is essential for understanding related signaling mechanisms. However, due to its chemical instability and the complexity of plant tissues, developing a reliable method for accurate quantification and real-time visualization of H2S in living plants remains challenging. Although fluorescent probes have been developed for H2S imaging, most still face critical limitations, such as emission in the visible region being susceptible to background fluorescence interference, and insufficient quantitative reliability of single-wavelength-based signal output modes in complex samples. Therefore, developing novel probes with near-infrared emission and rationetric response characteristics is of great significance.
Results
We constructed a near-infrared rationetric fluorescent probe, NIR-Cou-H2S, for H2S detection. The probe itself emits at 716 nm, and after specific reaction with H2S, a new emission peak appears at 552 nm, resulting in a distinct dual-emission rationetric response (716 nm/552 nm) accompanied by a visible color change. Using chemometrics-based fluorescence analysis, the probe successfully enabled direct quantitative detection of H2S in river and lake water samples. Its near-infrared emission effectively reduced interference from plant autofluorescence, thereby achieving high-contrast dual-channel fluorescence imaging. The probe was successfully applied for high-quality in situ visualization of H2S in living cells and tobacco seedling roots. More importantly, using NIR-Cou-H2S, we observed and recorded in real time the dynamic upregulation trend of endogenous H2S levels in tobacco roots under both drought and flooding stress conditions.
Significance
As a novel near-infrared rationetric probe, NIR-Cou-H2S provides a powerful tool for monitoring endogenous H2S dynamics in plants. Its characteristics significantly improve the reliability of imaging and quantification in complex plant samples, offering a key methodological approach for further elucidating the regulatory mechanisms of H2S in plant stress resistance.
硫化氢（H2S）是一种参与植物生理调节和逆境反应的重要内源气体传递素。监测其在植物体内的动态变化是了解相关信号机制的必要条件。然而，由于其化学不稳定性和植物组织的复杂性，开发一种可靠的方法来准确定量和实时可视化活植物中的H2S仍然是一个挑战。虽然已经开发出用于H2S成像的荧光探针，但大多数仍然面临着关键的局限性，例如可见光区域的发射容易受到背景荧光干扰，以及复杂样品中基于单波长的信号输出模式的定量可靠性不足。因此，开发具有近红外发射和定量响应特性的新型探针具有重要意义。结果构建了一种近红外定量荧光探针nir - cu -H2S，用于检测H2S。探针本身在716 nm处发射，与H2S特异反应后，在552 nm处出现新的发射峰，产生明显的双发射理性响应（716 nm/552 nm），并伴有可见的颜色变化。利用化学计量学为基础的荧光分析，探针成功地实现了对河流和湖泊水样中H2S的直接定量检测。其近红外发射有效降低了植物自身荧光的干扰，实现了高对比度双通道荧光成像。该探针已成功应用于活细胞和烟草幼苗根中H2S的高质量原位可视化。更重要的是，利用nir - cu -H2S，我们实时观察并记录了干旱和洪涝胁迫条件下烟草根系内源H2S水平的动态上调趋势。作为一种新型的近红外定量探针，nir - cu -H2S为监测植物内源H2S动态提供了强有力的工具。其特性显著提高了复杂植物样品成像和定量的可靠性，为进一步阐明H2S在植物抗逆性中的调控机制提供了关键的方法学途径。
{"title":"A near-infrared ratiometric fluorescent probe for visual sensing of H2S and monitoring its fluctuation in plant roots under drought and flooding stresses","authors":"Ruxuan Mo , Chao Kang , Jianping Yuan , Carl Redshaw , Ying Huang , Qilong Zhang , Xiufang Yan","doi":"10.1016/j.aca.2026.345144","DOIUrl":"10.1016/j.aca.2026.345144","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogen sulfide (H<sub>2</sub>S) is a key endogenous gasotransmitter involved in plant physiological regulation and stress responses. Monitoring its dynamic changes in plants is essential for understanding related signaling mechanisms. However, due to its chemical instability and the complexity of plant tissues, developing a reliable method for accurate quantification and real-time visualization of H<sub>2</sub>S in living plants remains challenging. Although fluorescent probes have been developed for H<sub>2</sub>S imaging, most still face critical limitations, such as emission in the visible region being susceptible to background fluorescence interference, and insufficient quantitative reliability of single-wavelength-based signal output modes in complex samples. Therefore, developing novel probes with near-infrared emission and rationetric response characteristics is of great significance.</div></div><div><h3>Results</h3><div>We constructed a near-infrared rationetric fluorescent probe, NIR-Cou-H<sub>2</sub>S, for H<sub>2</sub>S detection. The probe itself emits at 716 nm, and after specific reaction with H<sub>2</sub>S, a new emission peak appears at 552 nm, resulting in a distinct dual-emission rationetric response (716 nm/552 nm) accompanied by a visible color change. Using chemometrics-based fluorescence analysis, the probe successfully enabled direct quantitative detection of H<sub>2</sub>S in river and lake water samples. Its near-infrared emission effectively reduced interference from plant autofluorescence, thereby achieving high-contrast dual-channel fluorescence imaging. The probe was successfully applied for high-quality in situ visualization of H<sub>2</sub>S in living cells and tobacco seedling roots. More importantly, using NIR-Cou-H<sub>2</sub>S, we observed and recorded in real time the dynamic upregulation trend of endogenous H<sub>2</sub>S levels in tobacco roots under both drought and flooding stress conditions.</div></div><div><h3>Significance</h3><div>As a novel near-infrared rationetric probe, NIR-Cou-H<sub>2</sub>S provides a powerful tool for monitoring endogenous H<sub>2</sub>S dynamics in plants. Its characteristics significantly improve the reliability of imaging and quantification in complex plant samples, offering a key methodological approach for further elucidating the regulatory mechanisms of H<sub>2</sub>S in plant stress resistance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345144"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-22Epub Date: 2026-01-31DOI: 10.1016/j.aca.2026.345185
Yudong Wang, Yanxin Li, Yinan Zhan, Jie Yang, Yuxin Zhang, Lei Wang, Xiliang Luo
Electrochemical sensing techniques are still facing the challenges of biofouling and insufficient probe stability in complex biological matrices. To address this issue, we have designed a dl-serine Y-shaped peptide (YPEP) integrating the antifouling properties and recognition efficiency. Application demonstration was performed with detecting aminopeptidase N (APN) in human serum. The peptide-modified sensing interface exhibits effective antifouling performance, and enhanced resistance to enzymatic degradation. When detecting APN, the developed sensor showed a sensitive response within the linear range of 1.0 ng mL−1 to 10 μg mL−1 and a detection limit of 0.24 ng mL−1. Comparison studies were performed with the clinical human serum samples, and results obtained with the developed sensor and standard ELISA methods are in close agreement. This work provides a feasible strategy to developing robust antifouling peptide-based biosensing platforms for clinical applications in complex biological matrices.
电化学传感技术仍然面临着生物污染和探针在复杂生物基质中的稳定性不足的挑战。为了解决这一问题，我们设计了一种集防污性能和识别效率于一体的dl-丝氨酸y形肽（YPEP）。对检测人血清中氨肽酶N （APN）进行了应用演示。肽修饰的传感界面具有有效的防污性能，并增强了酶降解的抵抗力。该传感器在1.0 ~ 10 μg mL−1的线性范围内具有较高的灵敏度，检测限为0.24 ng mL−1。与临床人血清样本进行了比较研究，开发的传感器和标准ELISA方法获得的结果非常一致。这项工作为开发基于多肽的抗污染生物传感平台提供了一种可行的策略，可用于复杂生物基质的临床应用。
{"title":"A protease-resistant and anti-fouling electrochemical biosensing interface constructed with dl-serine modified Y-shaped peptides for reliable analysis of aminopeptidase N in human serum","authors":"Yudong Wang, Yanxin Li, Yinan Zhan, Jie Yang, Yuxin Zhang, Lei Wang, Xiliang Luo","doi":"10.1016/j.aca.2026.345185","DOIUrl":"10.1016/j.aca.2026.345185","url":null,"abstract":"<div><div>Electrochemical sensing techniques are still facing the challenges of biofouling and insufficient probe stability in complex biological matrices. To address this issue, we have designed a <span>dl</span>-serine Y-shaped peptide (YPEP) integrating the antifouling properties and recognition efficiency. Application demonstration was performed with detecting aminopeptidase N (APN) in human serum. The peptide-modified sensing interface exhibits effective antifouling performance, and enhanced resistance to enzymatic degradation. When detecting APN, the developed sensor showed a sensitive response within the linear range of 1.0 ng mL<sup>−1</sup> to 10 μg mL<sup>−1</sup> and a detection limit of 0.24 ng mL<sup>−1</sup>. Comparison studies were performed with the clinical human serum samples, and results obtained with the developed sensor and standard ELISA methods are in close agreement. This work provides a feasible strategy to developing robust antifouling peptide-based biosensing platforms for clinical applications in complex biological matrices.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345185"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146095760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-22Epub Date: 2026-01-22DOI: 10.1016/j.aca.2026.345110
Caitlin M. Tressler , Lauren DeVine , Rahul Bharadwaj , Dalton R. Brown , Daniel Weinberger , Kristine Glunde , Robert N. Cole
The human hippocampal trisynaptic circuit activity is essential for learning and memory. This canonical circuit has spatially distinct populations of neurons, but their unique contributions to neurodevelopment, as well as to dysfunction in neurodegenerative disorders, are missed when analyzing bulk tissue homogenates. Using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to guide laser capture microdissection (LCMD) of regions of interest for spatial multimodal analyses is a relatively new approach to study topographically distinct neuronal cell populations in heterogenous tissues. However, MALDI-MSI may not identify region-defining molecular mass-to-charge ions. Here, we apply a multimodal approach of MALDI-MSI-LCMD-lipidomic and proteomic analysis to the trisynaptic circuit. Our MALDI-MSI of the hippocampus revealed that the of mass-to-charge ions of the cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3) did not segment from the surrounding tissue. Thus, we developed a novel histology-guided MALDI-MS imaging-LCMD-spatial lipidomic/proteomic pipeline with four steps which does not rely on segmentation analysis to determine and co-register regions of interest in tissue sections. Our pipeline allows MALDI imaging, LCMD, lipidomic and proteomic analysis from the same tissue section and does not require co-registration across serial sections. In addition, poly-l-lysine coating for improving tissue/cell adherence on indium-tin-oxide microscopy slides did not impact MALDI-MSI or spatial proteomics. We show that the human trisynaptic circuit proteomes of CA1 and CA3 pyramidal neurons are more similar to each other than those of the dentate gyrus (DG), which is consistent with previously reported transcriptomics studies. The spatial distributions of several phospholipids and proteins, however, were significantly different in cell bodies from the CA1, CA3 and DG regions, and these lipids correlated with some lipid metabolizing enzymes in those regions. As little is known about lipid metabolism in the hippocampus, our pipeline provides an initial step in studying the combined and differential spatial regulation of the lipids and proteins within the trisynaptic circuit that will provide insights into the development and disease-related molecular changes in these important hippocampal regions.
{"title":"Histology-guided spatial lipidomics and proteomics of the trisynaptic circuit in the human hippocampus","authors":"Caitlin M. Tressler , Lauren DeVine , Rahul Bharadwaj , Dalton R. Brown , Daniel Weinberger , Kristine Glunde , Robert N. Cole","doi":"10.1016/j.aca.2026.345110","DOIUrl":"10.1016/j.aca.2026.345110","url":null,"abstract":"<div><div>The human hippocampal trisynaptic circuit activity is essential for learning and memory. This canonical circuit has spatially distinct populations of neurons, but their unique contributions to neurodevelopment, as well as to dysfunction in neurodegenerative disorders, are missed when analyzing bulk tissue homogenates. Using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to guide laser capture microdissection (LCMD) of regions of interest for spatial multimodal analyses is a relatively new approach to study topographically distinct neuronal cell populations in heterogenous tissues. However, MALDI-MSI may not identify region-defining molecular mass-to-charge ions. Here, we apply a multimodal approach of MALDI-MSI-LCMD-lipidomic and proteomic analysis to the trisynaptic circuit. Our MALDI-MSI of the hippocampus revealed that the of mass-to-charge ions of the cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3) did not segment from the surrounding tissue. Thus, we developed a novel histology-guided MALDI-MS imaging-LCMD-spatial lipidomic/proteomic pipeline with four steps which does not rely on segmentation analysis to determine and co-register regions of interest in tissue sections. Our pipeline allows MALDI imaging, LCMD, lipidomic and proteomic analysis from the same tissue section and does not require co-registration across serial sections. In addition, poly-<span>l</span>-lysine coating for improving tissue/cell adherence on indium-tin-oxide microscopy slides did not impact MALDI-MSI or spatial proteomics. We show that the human trisynaptic circuit proteomes of CA1 and CA3 pyramidal neurons are more similar to each other than those of the dentate gyrus (DG), which is consistent with previously reported transcriptomics studies. The spatial distributions of several phospholipids and proteins, however, were significantly different in cell bodies from the CA1, CA3 and DG regions, and these lipids correlated with some lipid metabolizing enzymes in those regions. As little is known about lipid metabolism in the hippocampus, our pipeline provides an initial step in studying the combined and differential spatial regulation of the lipids and proteins within the trisynaptic circuit that will provide insights into the development and disease-related molecular changes in these important hippocampal regions.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345110"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146033809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-22Epub Date: 2026-02-02DOI: 10.1016/j.aca.2026.345193
Longsheng Jin , YuJing Liu , Wenqing Xia , Meiling Zhou , Ziying Xiao , Jingyu Ge , Xiao Yuan , Qinqiang Zhu , Meisheng Wu
Background
Aflatoxin B1 (AFB1) is a highly toxic mycotoxin that poses serious threats to global food safety and public health. The ingestion of these contaminated products causes substantial risks to both food safety systems and public health. Despite the availability of various detection techniques, the requirement for highly sensitive analysis of AFB1 contamination in agricultural products continues to drive demand for improved methodologies.
Results
In this work, a novel electrochemiluminescence (ECL) biosensor was developed for the sensitive detection of aflatoxin B1 (AFB1) by integrating a galvanic replacement–synthesized Au@Ag–Pt nanozyme with a DNA walker–controlled rolling circle amplification (RCA) circuit. The core–shell Au@Ag–Pt nanozyme exhibits superior conductivity, enhanced peroxidase activity, and electrocatalytic performance, leading to a 17.67–fold amplification of ECL. A target-responsive “signal–off” mechanism was established based on AFB1-induced DNA structural switching and enzymatic cleavage, which effectively suppresses the RCA process and reduces the immobilization of nanozyme labels. The biosensor achieved a wide linear range from 5 × 10−11 to 5 × 10−5 mg/mL with a detection limit of 2.72 × 10−11 mg/mL. It shows high specificity against common interfering mycotoxins, good reproducibility, and satisfactory recoveries ranging from 82.7% to 110.5%.
Significance
This work not only provides a reliable platform for mycotoxin monitoring but also advances nanozyme engineering in biosensing, offering significant promise for food safety and environmental applications.
{"title":"Nicking-controlled biosensor: Integrating DNA Walker-RCA circuit with trimetallic nanozyme for aflatoxin B1 detection","authors":"Longsheng Jin , YuJing Liu , Wenqing Xia , Meiling Zhou , Ziying Xiao , Jingyu Ge , Xiao Yuan , Qinqiang Zhu , Meisheng Wu","doi":"10.1016/j.aca.2026.345193","DOIUrl":"10.1016/j.aca.2026.345193","url":null,"abstract":"<div><h3>Background</h3><div>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is a highly toxic mycotoxin that poses serious threats to global food safety and public health. The ingestion of these contaminated products causes substantial risks to both food safety systems and public health. Despite the availability of various detection techniques, the requirement for highly sensitive analysis of AFB<sub>1</sub> contamination in agricultural products continues to drive demand for improved methodologies.</div></div><div><h3>Results</h3><div>In this work, a novel electrochemiluminescence (ECL) biosensor was developed for the sensitive detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) by integrating a galvanic replacement–synthesized Au@Ag–Pt nanozyme with a DNA walker–controlled rolling circle amplification (RCA) circuit. The core–shell Au@Ag–Pt nanozyme exhibits superior conductivity, enhanced peroxidase activity, and electrocatalytic performance, leading to a 17.67–fold amplification of ECL. A target-responsive “signal–off” mechanism was established based on AFB<sub>1</sub>-induced DNA structural switching and enzymatic cleavage, which effectively suppresses the RCA process and reduces the immobilization of nanozyme labels. The biosensor achieved a wide linear range from 5 × 10<sup>−11</sup> to 5 × 10<sup>−5</sup> mg/mL with a detection limit of 2.72 × 10<sup>−11</sup> mg/mL. It shows high specificity against common interfering mycotoxins, good reproducibility, and satisfactory recoveries ranging from 82.7% to 110.5%.</div></div><div><h3>Significance</h3><div>This work not only provides a reliable platform for mycotoxin monitoring but also advances nanozyme engineering in biosensing, offering significant promise for food safety and environmental applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345193"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-22Epub Date: 2026-01-29DOI: 10.1016/j.aca.2026.345140
Iuliia A. Poimenova , Elizaveta Yu. Zhukova , Svetlana T. Ovseenko , Nikita S. Saratovsky , Ivan K. Kiryukhin , Daria-Mariia V. Ratova , Dmitrii M. Filatov , Anastasia M. Alekseeva , Svetlana V. Smirnova , Mikhail A. Proskurnin , Ivan V. Mikheev
Background
Graphene oxide (GO) is widely used in biomedicine and biotechnology due to its aqueous dispersibility and potential nanozyme activity. However, it remains analytically challenging because of structural heterogeneity and metallic inclusions, which affect its functional performance. GO therefore requires precise trace metal analysis, as even minor compositional variations can be critical. Modern multi-element analysis demands results that are both reliable and accurate, yet sound laboratory practice is not always followed. Although ICP-OES offers high sensitivity and precision, metrological rigor is often lacking. This work addresses the need for validated and robust elemental analysis of GO bulk material and particle-size fractions.
Results
ICP-OES was employed to quantify metals in bulk GO and to characterize Mn and Ti distribution in GO fractions. A fast algorithm was developed to identify robust plasma conditions by mapping the Mermet coefficient (Mg II/Mg I intensity ratio) across a range of flow rates and RF power levels. Concentrations for selected elements in the bulk material ranged from 10−1 to 103 mg kg−1; in aqueous dispersions, values reached up to 103 mg L−1. Validation parameters for Mn, Ti, and Fe included recoveries of 85–115 %, detection limits of 0.1–0.3 ng kg−1 (for used weight ca. 50 mg), and intra-/inter-day RSDs not exceeding 7 %. The Mermet coefficient exhibited an RSD of ∼0.6 %, indicating minimal matrix interference. Fraction analysis revealed a monotonic decrease in Mn toward fractions with smaller lateral-size cut-offs. ATR-FTIR spectra were obtained for stirred, purified, and ultrasound-treated GO fractions to assess whether purification altered the surface functional groups.
Significance
This study provides a validated approach for trace metal analysis in GO, combining sensitivity, robustness, and reproducibility across both bulk solid and aqueous dispersion forms. By experimentally examining the influence of plasma conditions on analytical performance, it offers the first systematic assessment of GO-specific ICP-OES parameter optimization. The results support more reliable characterization of GO materials and enable improved quality control in applications where elemental composition critically affects functionality.
氧化石墨烯（GO）由于其水溶性和潜在的纳米酶活性而广泛应用于生物医学和生物技术。然而，由于结构非均质性和金属夹杂物影响了其功能性能，因此在分析上仍然具有挑战性。因此氧化石墨烯需要精确的痕量金属分析，因为即使是微小的成分变化也可能是至关重要的。现代多元素分析要求结果既可靠又准确，但并不总是遵循良好的实验室实践。虽然ICP-OES具有较高的灵敏度和精度，但通常缺乏计量严谨性。这项工作解决了氧化石墨烯散装材料和粒度分数的验证和稳健元素分析的需要。结果icp - oes测定了大块氧化石墨烯中的金属，并表征了氧化石墨烯馏分中Mn和Ti的分布。通过绘制Mermet系数（Mg II/Mg I强度比）在流量和射频功率水平范围内的映射，开发了一种快速算法来识别稳健的等离子体条件。散装材料中选定元素的浓度范围为10 - 1至103 mg kg - 1；在水相分散体中，该值可达103 mg L−1。Mn、Ti和Fe的验证参数回收率为85 - 115%，检出限为0.1-0.3 ng kg - 1（使用重量约为50 mg），日内/日间rsd不超过7%。Mermet系数的RSD为~ 0.6%，表明基质干扰最小。分数分析显示，Mn的单调下降趋向于横向截距较小的分数。获得搅拌、纯化和超声处理氧化石墨烯馏分的ATR-FTIR光谱，以评估纯化是否改变了表面官能团。本研究为氧化石墨烯中痕量金属的分析提供了一种有效的方法，结合了敏感性、稳健性和可重复性，可以跨越大块固体和水分散形式。通过实验研究等离子体条件对分析性能的影响，首次对go特异性ICP-OES参数优化进行了系统评估。研究结果支持更可靠的氧化石墨烯材料表征，并在元素组成严重影响功能的应用中提高质量控制。
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Pub Date : 2026-03-22Epub Date: 2026-01-29DOI: 10.1016/j.aca.2026.345173
Jian-Ming Liu , Jian Liu , Tian-Tian Ma , Cheng-Xiong Yang
Background
Microporous organic networks (MONs) are an emerging class of porous materials characterized by extremely large surface area, tunable topology, and superior stabilities, which have received increasing attention in sample pretreatment. However, their superhydrophobic nature has largely limited their application scope. The synthesis of functionalized MONs has proved to be a feasible strategy to broaden their utility. Nevertheless, this approach remains constrained by the limited variety and availability of functionalized monomers. Therefore, developing novel post-synthetic modification strategies is crucial for expanding the functionality and applications of MONs, particularly in trace analyte enrichment.
Results
Herein, we present a novel post-esterification strategy to synthesize a new carboxyl groups enriched magnetic MON (MMON-2COOH) for the highly efficient magnetic solid-phase extraction (MSPE) of four quaternary ammonium alkaloids (QAAs) from complex urine samples. The resulting MMON-2COOH not only retains the porous structure, excellent solvent and thermal stability, and rapid magnetic responsiveness of its precursor (hydroxyl-functionalized magnetic MON) but also incorporates conjugated networks and abundant carboxyl groups. This enables efficient extraction of QAAs through multiple interactions, including π-π stacking, hydrogen bonding, and electrostatic attraction. After optimization of extraction factors, an analytical method integrating MMON-2COOH-based extraction with HPLC-UV detection was established, achieving sensitive and selective determination of QAAs with low limits of detection of 0.05–0.5 μg L−1 and limits of quantification of 0.5–1.0 μg L−1, wide linear range of 0.5–1000 μg L−1, negligible matrix effect of 0.88–1.09, rapid extraction of 2 min, and less adsorbent consumption of 2 mg.
Significance
This is the first example of post-esterification preparation of carboxyl groups functionalized MON. The results demonstrate that MMON-2COOH is a highly effective adsorbent, and the developed method is suitable for accurate determination of QAAs in biological samples. This work highlights the significant potential of post-esterification as a versatile approach for designing high-performance adsorbents in sample pretreatment.
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