Analytica Chimica Acta最新文献

{"title":"Systematic characterisation of microplastics released from disposable medical devices using laser direct infrared spectroscopy","authors":"Chuanfeng Chen,&nbsp;Shanshan Du,&nbsp;Ziyan Liu,&nbsp;Wenfei Li,&nbsp;Fangbiao Tao,&nbsp;Xuejiao Qie","doi":"10.1016/j.aca.2025.343982","DOIUrl":"10.1016/j.aca.2025.343982","url":null,"abstract":"<div><h3>Background</h3><div>Human exposure to microplastics (MPs) is widespread, attracting significant attention from both the public and the scientific community. Although several direct and indirect exposure pathways have been investigated, the extent of MP exposure from disposable medical devices remains poorly understood and warrants further research.</div></div><div><h3>Results</h3><div>This work indicates that many MPs (10-30 μm) were released during the simulated use of disposable medical devices. Two common medical devices–disposable infusion tubes and blood needles–were selected as the research subjects. Analysis utilizing laser direct infrared (LDIR) revealed that plastic released from infusion tubes primarily consisted of polyamide (PA), polyvinyl chloride (PVC), and polyethene terephthalate (PET), with an average total number (ATN) of 11.8 particles/mL. MPs released from blood collection needles mainly consisted of polyurethane (PU) and PET, with an ATN of 82.7 particles/mL. For a 0.9% normal saline, the ATN released from the infusion tubes during the stimulating infusion scenario at room temperature (4 h) was approximately 16 particles/mL, primarily consisting of PA, PVC, and PET. Additionally, the release of MPs increased with rising temperatures. Under the same conditions, ATN release from the blood collection needles was approximately 84.4 particles/mL, mainly from PA, PVC, and PU.</div></div><div><h3>Significance</h3><div>This implies that MPs can enter the bloodstream directly through infusion tubes and blood collection needles, highlighting the need for greater attention to the risk of patient exposure.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343982"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen peroxide regulated split-type electrochemiluminescence sensing platform for non-invasive detection of gastric cancer-associated D-amino acids","authors":"Juan Li ,&nbsp;Ming-Chun Lai ,&nbsp;Ya-Meng Zhong ,&nbsp;Ya-Ling Chen ,&nbsp;Na Wu ,&nbsp;Wei Chen ,&nbsp;Hua-Ping Peng","doi":"10.1016/j.aca.2025.344010","DOIUrl":"10.1016/j.aca.2025.344010","url":null,"abstract":"<div><div>Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective <span>d</span>-alanine (D-Ala) ECL assay strategy via a turn-off-on split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H₂O₂) could be used as an efficient etching agent to turn on the MnO₂/gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H₂O₂. Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0 × 10 <strong>⁻</strong>¹⁰∼1.0 × 10<strong>⁻</strong>³ mol L<strong>⁻¹</strong>, and realized the detection of D-Ala as low as 2.2 × 10<strong>⁻</strong>¹¹ mol L<strong>⁻¹</strong> (S/N = 3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H₂O₂ to MnO₂ nanomaterials on electrode surface. In our perception, this H₂O₂ mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344010"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotary fluorescence array microfluidic chip for rapid detection of multiple pathogens in foods using dual-discs assisted recycling for signal amplification","authors":"Bailu Liu ,&nbsp;Zhenli Xu ,&nbsp;Zhijian Jia ,&nbsp;Huihui Wu ,&nbsp;Tianhua Li ,&nbsp;Zhenzhong Yu ,&nbsp;Ning Gan","doi":"10.1016/j.aca.2025.344014","DOIUrl":"10.1016/j.aca.2025.344014","url":null,"abstract":"<div><h3>Background</h3><div>The ability to rapidly and on-site detect multiple pathogens in food is crucial for food safety monitoring. The microfluidic chip integrated with aptamer (Apt) probes stands as a powerful tool for this venture. However, the utility of these chips has been constrained by the need for cumbersome fluid control devices, such as syringe pumps and bulky optical detectors, so the development of highly integrated integrated chips has become one of the priorities of biosensor development in recent years.</div></div><div><h3>Result</h3><div>In this work, a novel rotary fluorescence array microfluidic chip has been engineered for the simultaneous and rapid detection of three pathogens. The chip has three different detection regions and a double-layer structure composed of PDMS and glass. The Apts and its complementary DNA strands were immobilized on the gold nanoparticle layer in the detection region for pathogen identification, the fluorescence (FL) from the double-stranded staining solution added after the reaction was used for signal amplification. Different arrays of gold discs can be employed to identify various bacteria. This method enables the detection of <em>Staphylococcus aureus</em> (<em>S.A</em>), <em>Salmonella typhimurium</em> (<em>S.T</em>), and <em>Vibrio parahemolyticus</em> (<em>V.P</em>) in food samples within 30 min at a sensitivity of 7–39 CFU/mL.</div></div><div><h3>Significance</h3><div>The microfluidic chip has good specificity and sensitivity while avoiding cross interference, and the ratio form of quantification avoids background light interference. Different arrays can be used to identify different bacteria, which is of great significance for the rapid detection of a variety of foodborne pathogens in the field.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1356 ","pages":"Article 344014"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-well colorimetric sensor array for discrimination and smartphone-assisted detection of catecholamines based on Fe-carbon dots nanozymes","authors":"Guang-Ming Bao,&nbsp;Dan-Dan Chen,&nbsp;Yi-Fan Xia,&nbsp;Zhi-Qiang Cai,&nbsp;Shun-Qiang Cui,&nbsp;Xia Wei,&nbsp;Zhen-Chong Dou,&nbsp;Yuan Yuan,&nbsp;Akimana Sandra,&nbsp;Hou-Qun Yuan","doi":"10.1016/j.aca.2025.343997","DOIUrl":"10.1016/j.aca.2025.343997","url":null,"abstract":"<div><h3>Background</h3><div>Catecholamines (CAs), such as noradrenaline (NE), adrenaline (AD), and dopamine (DA), are essential signaling mediators that regulate various physiological functions. Monitoring their levels is crucial for studying and diagnosing diseases, as abnormal concentrations are associated with numerous health conditions. However, distinguishing between these CAs is challenging due to their highly similar molecular structures.</div></div><div><h3>Results</h3><div>In this study, Fe-doped carbon dot-based nanozymes (<strong>Fe-CDs</strong>) with strong peroxidase-like activity were synthesized using a simple one-pot method. <strong>Fe-CDs</strong>-based sensing systems exhibit excellent stability, reproducibility, sensitivity (with detection limits of 26.6 nM for NE, 46.0 nM for AD, and 33.3 nM for DA), and anti-interference properties. A triple-channel single-well colorimetric sensor array was developed by collecting the absorbance at 20, 40, and 60 min as sensing units, enabling the effective differentiation and identification of various CAs.</div></div><div><h3>Significance</h3><div>The <strong>Fe-CDs</strong>-based system has proven capable of detecting CAs in real human urine and fetal bovine serum. Additionally, the <strong>Fe-CDs</strong>-based smartphone-assisted platform provides efficient, highly sensitive, and on-site CAs detection, making it highly promising for biomedical and diagnostic applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343997"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of urinary stones using near-infrared spectroscopy and chemometrics: A promising method for intraoperative application","authors":"Ekaterina Boichenko ,&nbsp;Mikhail Paronnikov ,&nbsp;Anna Reznichenko ,&nbsp;Dmitry Korolev ,&nbsp;Vladimir Protoshchak ,&nbsp;Dmitry Kirsanov","doi":"10.1016/j.aca.2025.344007","DOIUrl":"10.1016/j.aca.2025.344007","url":null,"abstract":"<div><div>In low-invasive surgical treatment of urolithiasis, there is a need for an analytical method to determine the chemical composition of urinary stones in real-time mode, i.e., intraoperatively. While a thorough phase analysis can be done after the surgery, preliminary information about a target stone would be helpful for the specialists for choosing an optimal strategy of treatment and giving some immediate dietary or drug prescriptions to a patient. Near-infrared spectroscopy (NIRS) is a good candidate for such a method that can provide immediate results without obligatory sample preparation. Fiber optic probes, often used for acquiring near-infrared spectra, are compatible with surgical instrumentation. Chemometric algorithms can successfully resolve the complexity of NIR spectra, which consist of overlapped signals. For the first time, we applied NIRS in diffuse reflectance mode to classify three major types of urinary stones: oxalates, urates, and phosphates. To imitate the real conditions of a surgery, the NIR spectra were acquired not only under ambient conditions but also in saline medium. A trained and optimized multinomial classifier (Error Correcting Output Codes) showed an acceptable precision and recall for an independent validation dataset. Even considering the strong absorbance of saline, the calculated geometric mean was 94 %, 87 %, and 71 % for oxalates, urates, and phosphates, respectively. A first real-time approbation during a real surgery (percutaneous nephrolithotomy) demonstrated a compatibility of the suggested approach with the surgical protocols and a good agreement of the acquired NIR spectra and the results of reference X-ray phase analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344007"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmon-enhanced fluorescence sensor based on Au nanocages for sensitive detection of norepinephrine","authors":"Chenye Xu ,&nbsp;Shilin Chen ,&nbsp;Wen Sun,&nbsp;Rong Xu,&nbsp;Xiangyuan Yin,&nbsp;Dongsheng Guo,&nbsp;Yaqiong Yang","doi":"10.1016/j.aca.2025.343995","DOIUrl":"10.1016/j.aca.2025.343995","url":null,"abstract":"<div><h3>Background</h3><div>Norepinephrine (NE) as a crucial monoamine neurotransmitter in the central and sympathetic nervous system, plays an important role in different physiological and pathophysiological processes. Brain NE can modulate cerebrospinal fluid flux and neurovascular coupling, regulate cortical and hippocampal neuronal circuitry, and participate the immune system. In addition, the reduced concentration of NE in brain was currently deemed to be the internal reason of major depression. However, development of detection method of NE with high spatiotemporal resolution in living systems remains a great challenge.</div></div><div><h3>Results</h3><div>Herein, a plasmon-enhanced fluorescence (PEF) sensor based on Au nanocages (Aucages) were designed and prepared for ultra-sensitive detection of NE. Aucages with porous walls, hollow interior and systematically tunable localized surface plasmon resonance (LSPR) wavelengths (536 nm, 654 nm, 754 nm) were prepared to obtain a highly fluorescent enhancement of Au nanoclusters (Au NCs). Moreover, polyethylene glycol (PEG) with different molecular weight (1000, 5000, 10000 Da) were applied to control the distance between the Aucages and Au NCs. 3D-FDTD simulation results indicated that the fluorescence enhancement was primarily due to the internal and external enhanced electric field effects of Aucages. This sensor was applied for the turn-on detection of NE in commonly used clinical injectable norepinephrine bitartrate with the recovery rate of 98.06–105.34 %. Meanwhile, real-time fluorescence imaging of NE in living pheochromocytoma (PC-12) cells was explored with a red-emitted fluorescence.</div></div><div><h3>Significance</h3><div>This study first employed Aucages with more “hot spot” for red-emitted Au NCs to realize fluorescence enhancement. It provides a new method for the development of more sensitive, accurate and convenient analysis of NE in clinical drug analysis, cell monitor and metabolism study.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 343995"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel biosensor MDC@N-MMCNs to selective detection and elimination of foodborne bacterial pathogens","authors":"Shuyue Sun ,&nbsp;Yonglin Feng ,&nbsp;Haonan Li ,&nbsp;Sijia Xu ,&nbsp;Huijuan Huang ,&nbsp;Xuan Zou ,&nbsp;Ziquan Lv ,&nbsp;Xiangjie Yao ,&nbsp;Shuiqing Gui ,&nbsp;Yinghua Xu ,&nbsp;Xiaobao Jin ,&nbsp;Xuemei Lu","doi":"10.1016/j.aca.2025.344008","DOIUrl":"10.1016/j.aca.2025.344008","url":null,"abstract":"<div><h3>Background</h3><div>Infections caused by foodborne pathogens pose a major threat to human health. Traditional bacterial detection methods, such as plate culture and polymerase chain reaction, cannot meet the growing demand for fast and accurate detection. In contrast, colorimetric sensors have the characteristics of convenience, speed, and visualization, but their specific sensitivity is relatively poor. Therefore, it is necessary to develop a biosensor with selective identification of foodborne pathogens, high sensitivity, and early detection of foodborne pathogen contamination in food.</div></div><div><h3>Results</h3><div>We have developed a broad-spectrum microbial detection biosensor platform MDC@N-MMCNs that combines antimicrobial peptides as identifying ingredients with mesoporous carbon with peroxidase-like activity to detect and eliminate foodborne pathogens rapidly. In this study, nitrogen-doped magnetic mesoporous carbon nanospheres (N-MMCNs) were prepared using ferric nitrate as the magnetic source. <em>Musca domestica</em> cecropin (MDC) has abundant recognition sites on the surface of bacteria, which helps to recognize and amplify the signal, and combines with N-MMCNs to form MDC@N-MMCNs. MDC@N-MMCNs have high stability, specificity, and sensitivity, with a visual detection limit as low as 10² CFU/mL. The MDC@N-MMCNs paper-based sensor enables selective and rapid detection of four foodborne pathogens via a smartphone application.</div></div><div><h3>Significance</h3><div>Based on these findings, we believe that MDC@N-MMCNs hold great potential for on-site bacterial infection diagnosis in resource-limited environments or point-of-care (POCT) settings, offering a simple, cost-effective solution for food safety and public health.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344008"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AFM-optimized single-cell level LA-ICP-MS imaging for quantitative mapping of intracellular zinc concentration in immobilized human parietal cells using gelatin droplet-based calibration","authors":"Valerie Boger ,&nbsp;Philip Pirkwieser ,&nbsp;Noreen Orth ,&nbsp;Melanie Koehler ,&nbsp;Veronika Somoza","doi":"10.1016/j.aca.2025.343999","DOIUrl":"10.1016/j.aca.2025.343999","url":null,"abstract":"<div><h3>Background</h3><div>Quantitative bioimaging of trace elements at the single-cell level is crucial for understanding cellular processes, including metal uptake and distribution. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has emerged as a gold standard for elemental bioimaging due to its high sensitivity and spatial resolution. However, calibration remains challenging due to the lack of homogeneous biological standards. This study addresses these challenges by introducing a gelatin-based calibration strategy optimized for Zn mapping in human parietal cells. By minimizing heterogeneity in gelatin standards and optimizing laser ablation conditions, the approach ensures accurate and reproducible results for cellular bioimaging.</div></div><div><h3>Results</h3><div>A gelatin-based calibration strategy for LA-ICP-MS was developed to quantify intracellular Zn at a single-cell level in human parietal cells. Preparation conditions for gelatin standards were optimized to minimize heterogeneity, eliminating the need for entire droplet ablation and significantly reducing analysis time. Atomic force microscopy (AFM) was employed to optimize laser ablation conditions and determine ablated volumes, ensuring quantitative Zn detection. The method demonstrated high linearity (R² &gt; 0.99) and reproducibility. Application of the calibration strategy to ZnCl₂-treated parietal cells revealed Zn distribution at a cellular level, visualized using a 5 μm laser beam. Integration with bright field imaging enabled the exclusion of apoptotic cells and debris, ensuring robust analysis. Validation with bulk ICP-MS showed excellent agreement, confirming the method's reliability and potential for high-resolution bioimaging.</div></div><div><h3>Significance</h3><div>This work introduces a robust and reproducible calibration strategy for quantitative elemental bioimaging using LA-ICP-MS. It details the preparation of a gelatin matrix with a homogeneous element distribution, serving as an alternative to using biological material and significantly reducing analysis time. Laser ablation parameters were optimized using AFM to ensure quantitative ablation, which is necessary for calibration through LA-ICP-MS imaging. This approach provides a powerful tool for studying trace element dynamics in single cells and holds potential for diverse biological and biomedical applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343999"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorine-functionalized black phosphorus quantum dots induced superoxide anion generation and depletion for efficient chemiluminescence detection","authors":"Hui Gong,&nbsp;Dayang Zhao,&nbsp;Houjing Liu","doi":"10.1016/j.aca.2025.343991","DOIUrl":"10.1016/j.aca.2025.343991","url":null,"abstract":"<div><h3>Background</h3><div>Due to their unique optoelectronic properties, environmental friendliness, and excellent biocompatibility, metal-free quantum dots have been a new star in exploring novel chemiluminescence (CL) systems for analytical applications in recent years. However, unknown CL property, relatively weak emission and instability of some of them in water (eg. black phosphorus) often seriously hindered their further applications. Hence, developing a novel QDs-assist CL signal amplification to achieve efficient analyst detection is significant and currently hot topic for researchers.</div></div><div><h3>Results</h3><div>In this work, we purposely synthesized chlorine-functionalized black phosphorus quantum dots (Cl-BPQDs) with improved stability and rich-hole property, which were demonstrated to exhibit the excellent capability for the activation of ferrate (VI) with large reactive oxygen species generation and leading to enhanced CL signal. The detail mechanism was demonstrated, the unique CL response to the presence of active sites (P–Cl) in Cl-BPQDs, which accelerated ferrate (VI) decomposition and produced a large amount of superoxide anion (^•O₂⁻). And then, the radiative recombination of the exogenous electron-donated and existing holes Cl-BPQDs accounting for the strong CL emission. Furthermore, based on the consumption capacity of ascorbic acid (AA) and glutathione (GSH) for ^•O₂⁻, a direct CL sensing platform of Cl-BPQDs/ferrate (VI) quenching was fabricated to AA and GSH detection. This fabricated assay has broad detection linear ranges (2–200 μM) and low detection limit (GSH: 1.3 μM; AA: 1.7 μM). Compared with the reported CL technique, this new method displayed superior sensitivity and anti-interference capabilities toward transition-metal ions and inorganic anions. The potential analytical application of the new CL system was further demonstrated by the evaluation of total antioxidant capacity (TAC) in diabetic patients.</div></div><div><h3>Significance</h3><div>This study proposes a new strategy for enhancing CL signal via Cl-BPQDs triggering ^•O₂⁻ generation and depletion, which provides an innovative tool for ascorbic acid and glutathione detection. This method not only enriches our understanding of the optical characteristics of BP, but also provides a new charge transfer-based path for CL amplification.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 343991"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of medium- and long-chain perfluorocarboxylic acids in water by catalytic methylation with trimethylsilyl diazomethane and gas chromatography - Mass spectrometry analysis","authors":"Guoliang Huang ,&nbsp;Baoshan Zhang ,&nbsp;Yunna Li ,&nbsp;Guohua Zhu ,&nbsp;Kezhi Jiang","doi":"10.1016/j.aca.2025.344001","DOIUrl":"10.1016/j.aca.2025.344001","url":null,"abstract":"<div><div>Perfluorocarboxylic acids (PFCAs) have received wide public concern, due to their biotoxicity and universality. However, the current LC-MS method suffers from the background interference originating from the instrumental system and the time-consuming procedure in analyzing long-chain PFCAs. In this work, we established a rapid method for the determination of medium- and long-chain PFCAs based on derivatization with trimethylsilyl diazomethane (TMSD) and GC-MS analysis. Under the catalysis of trimethylchlorosilane, methylation of trace PFCAs with TMSD was fully achieved within only 2 min, which represents inaugural efficient methylation of carboxylic acid. The methylated products of 9 PFCAs (C7–C18 except C15 and C17) were determined by GC-MS/MS within a GC run of 13 min. Moreover, this method successfully avoided the background interference in the HPLC-MS analysis, which made analytical results more reliable. The quantitative linear range was 0.1–100 ng/mL for medium PFCAs (C8 to C13), 1–1000 ng/mL for C7 and long-chain PFCAs (C14 to C18), respectively. Good reproducibility was obtained for all PFCAs, with an intra-day RSD of 0.90–4.35 % and an inter-day RSD of 2.62–5.64 % at 5 ng/mL. The recovery rate was 83.2–111.3 % at 10 ng/mL, indicating the accuracy and reliability of these results. These results indicated that this method provided a practical and reliable method for the detection of medium- and long-chain perfluorocarboxylic acids in water samples by GC-MS analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344001"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}