Analytica Chimica Acta最新文献

{"title":"In-situ laser-induced metal (M = Fe, Co, Ni, Cu)-doped graphene electrode for sensitive uric acid sensing in sweat","authors":"Nannan Ma ,&nbsp;Qilin Liu ,&nbsp;Nianyang Cai ,&nbsp;Fangfang Zhu ,&nbsp;Ning Gan ,&nbsp;Zhenzhong Yu","doi":"10.1016/j.aca.2026.345130","DOIUrl":"10.1016/j.aca.2026.345130","url":null,"abstract":"<div><h3>Background</h3><div>Laser-induced graphene (LIG) technology has demonstrated considerable promise for the fabrication of wearable electrochemical sensing devices. However, the pristine LIG typically exhibits suboptimal electrochemical performance, often necessitating heteroatom doping to enhance its functionality. Among these strategies, metal doping has proven particularly effective in significantly boosting sensing capabilities. Conventional doping approaches, however, are frequently hampered by cumbersome pretreatment procedures or weak metal-graphene interfacial interaction, thereby restricting their practical efficacy. Therefore, novel synthetic methodologies are urgently needed for efficient metal-doped LIG fabrication.</div></div><div><h3>Results</h3><div>This study developed an <em>in-situ</em> laser-induced metal-doped graphene (M-LIG, M = Fe, Co, Ni, Cu) fabrication method. It involved plasma-treating polyimide films to introduce hydrophilic groups for metal ion adsorption, followed by CO₂ laser irradiation that achieved atomic-scale metal doping during graphene formation. A systematic investigation of preparation parameters and metal dopant types revealed their significant effects on the morphology, structure, and electrochemical performance of the resulting LIG. These materials were used to construct a portable electrochemical detection system for highly sensitive uric acid monitoring in sweat. Results showed that M-LIG sensors outperformed pristine LIG, with Ni-LIG exhibiting optimal performance of a 10.6-fold reduction in detection limit and a 5-fold enhancement in the upper linear detection range. Density functional theory calculations indicated that Ni active sites played a crucial role in enhancing sensing performance by facilitating analyte adsorption and electron transfer.</div></div><div><h3>Significance</h3><div>This study presents a universal fabrication strategy for M-LIG, which significantly streamlines the preparation process of LIG and enhances its sensing performance. Demonstrating broad application prospects in electrode surface engineering for wearable electrochemical biosensors, this approach paves a new avenue for the development of advanced devices aimed at precise personalized health monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1390 ","pages":"Article 345130"},"PeriodicalIF":6.0,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146001444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible hydrophilic-hydrophobic array detection patch integrated with Au NPs/Cu-TCPP(Fe) biomimetic cascade catalysis reactions for enzyme-free sweat glucose monitoring","authors":"Chen Yang,&nbsp;Limin Zhang,&nbsp;Zijun Ma,&nbsp;Junhao Gao,&nbsp;Yang Zhou,&nbsp;Bing Li,&nbsp;Jianbo He,&nbsp;Fang Li","doi":"10.1016/j.aca.2026.345105","DOIUrl":"10.1016/j.aca.2026.345105","url":null,"abstract":"<div><h3>Background</h3><div>The detection and quantification of glucose in human biofluids are critical for the early diagnosis and management of metabolic disorders such as hypoglycemia and diabetes. Non-invasive wearable sensors for sweat glucose monitoring have emerged as promising alternatives to traditional blood glucose testing due to their ease of use, rapid response, and portability. However, the development of stable, cost-effective, and enzyme-free wearable platforms capable of reliable sweat glucose detection remains a significant challenge. Current enzyme-based sensors often suffer from poor stability under ambient conditions, complex fabrication processes, and high costs, limiting their widespread applicability in resource-constrained settings.</div></div><div><h3>Results</h3><div>Au NPs/Cu-TCPP(Fe) nanozymes exhibiting dual peroxidase-like and glucose oxidase-like catalytic activities were synthesized and characterized. These nanozymes catalyze a cascade reaction: glucose oxidation generates H₂O₂, which is then decomposed to produce hydroxyl radicals that oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), yielding a blue-colored product for colorimetric detection. A flexible, multi-layer hydrophilic-hydrophobic array patch was fabricated by integrating the nanozymes and TMB into a paper-based matrix. This design enables efficient in situ sweat collection, concentration, and colorimetric analysis. The patch exhibits a linear detection range of 20–200 μM with a detection limit of 12.72 μM, suitable for physiological sweat glucose levels. Selectivity tests confirmed negligible interference from common sweat components. A smartphone application was developed to enable real-time, quantitative analysis of colorimetric signals. Clinical validation on multiple volunteers across different skin locations (chest, back, forearm, forehead) showed strong correlation with reference methods (HPLC-MS, commercial glucose detection kit), with relative errors &lt;10 %.</div></div><div><h3>Significance</h3><div>This study presents a low-cost and enzyme-free sweat glucose detection platform based on Au NPs/Cu-TCPP(Fe) nanozymes. The integration of smartphone technology enables point-of-care testing, making the platform particularly suitable for personalized health monitoring. The successful validation in human subjects demonstrates its potential for real-world applications in diabetes management and early disease detection. This work bridges the gap between nanozyme catalysis and wearable sensor technology, providing a versatile platform for non-invasive biomarker detection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1389 ","pages":"Article 345105"},"PeriodicalIF":6.0,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145995956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-Need and Portable Sensing Strategies for Quality and Safety Assessment of Traditional Chinese Medicine Products: A Critical Analytical Review","authors":"Zihua Wang, Beibei Liu, Altaf Hussain, Zhongke Sun, Le Wang, Peng Li","doi":"10.1016/j.aca.2026.345119","DOIUrl":"https://doi.org/10.1016/j.aca.2026.345119","url":null,"abstract":"Sensor technology has emerged as a transformative tool for point-of-need and portable quality control and safety assessment of Traditional Chinese Medicine (TCM) products. Although separation and detection technologies have improved, there is still a strong need to critically evaluate the entire analytical workflow from sample preparation to data interpretation to meet regulatory and routine monitoring requirements. This review critically evaluates the complete analytical workflow—from sample pretreatment to intelligent data interpretation—necessary to bridge the gap between laboratory prototypes and real-world deployment. Additionally, it provides a systematic summary of the mechanisms and applications of common types of sensors, utilizing nanomaterials and molecular recognition elements to overcome the specific challenge of complex TCM matrices. A particular focus is placed on the emerging roles of Artificial Intelligence (AI) and Machine Learning (ML) in resolving high-dimensional spectral data and enhancing pattern recognition accuracy. Furthermore, we critically analyze current bottlenecks, including matrix interference, the lack of standardized validation protocols, and the reliability of portable devices in uncontrolled environments. Future directions are highlighted towards the development of self-validating, cloud-integrated (IoT), and eco-friendly sensing systems, aiming to support the global standardization and safety assurance of TCM products.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"37 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145993126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput copper speciation with SEC-UV-ICPMS: Insights from Wilson's disease mouse models","authors":"Yu Zhang ,&nbsp;Xueqing Zhao ,&nbsp;Beth Crowell ,&nbsp;Heinrich Lob ,&nbsp;Sarah Hatsell ,&nbsp;Aris N. Economides ,&nbsp;Evangelos Pefanis ,&nbsp;Jikang Wu ,&nbsp;Haibo Qiu ,&nbsp;Ning Li","doi":"10.1016/j.aca.2026.345113","DOIUrl":"10.1016/j.aca.2026.345113","url":null,"abstract":"<div><h3>Background</h3><div>Wilson's disease (WD) is a genetic disorder caused by <em>ATP7B</em> gene mutations, impairing copper excretion and leading to toxic copper accumulation in vital organs. Inductively coupled plasma mass spectrometry (ICPMS)-based serum copper analysis has shown reliability in WD studies, with relative exchangeable copper (REC, proportion of exchangeable copper in the blood relative to the total copper) and accurate non-ceruloplasmin-bound copper (ANCC) emerging as promising biomarkers. However, challenges persist in applying these methods in live animal studies because of limited sample volumes and impractical copper specifications, necessitating alternative analytical approaches.</div></div><div><h3>Results</h3><div>This study comprehensively evaluated a size-exclusion chromatography coupled with ultraviolet (SEC-UV)-ICPMS method for precise copper speciation in mouse serum. This method effectively separated major copper-binding proteins while minimizing sample preparation and consumption. As a comparison, the direct injection-based ICPMS method was optimized to determine relative exchangeable copper (REC), and strong anion exchange (SAX)-ICPMS-based copper speciation approaches were investigated to validate the accuracy of the SEC-UV-ICPMS method, further confirming its robustness for live mouse studies. Further application to WD mouse models (ATP7B^tx−J) revealed distinct copper distribution differences between diseased and healthy states. Relative non-ceruloplasmin-bound copper (RNCC) was newly identified as a promising potential biomarker, defining a diagnostic threshold of 52–58 %. By accurately quantifying copper species in mouse serum, this study established a reliable analytical framework that greatly improved our understanding of copper distribution in Wilson's disease research. Our approach demonstrated high specificity, reproducibility, and throughput, suitable for live mouse studies.</div></div><div><h3>Significance</h3><div>The SEC-UV-ICPMS platform offers a robust and efficient approach for serum copper speciation and quantification that addresses current limitations in live animal studies. By establishing RNCC as a reliable potential biomarker and enabling detailed copper profiling, this method enhances the precision of copper measurement and supports therapeutic monitoring. The applicability to WD research and capability of absolute quantification of copper species underscore its potential as a critical tool in clinical diagnostics and mechanistic studies, advancing the understanding and treatment of copper metabolism disorders.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1390 ","pages":"Article 345113"},"PeriodicalIF":6.0,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145993301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T4 DNA ligase-mediated RAA coupled with RNA aptamer-driven cascade signal amplification for ultra-sensitive monkeypox virus detection","authors":"Chenxi Li ,&nbsp;He Sun ,&nbsp;QingWen Jia ,&nbsp;Zebin Zhang ,&nbsp;Shengjun Bu ,&nbsp;Hongtao Zhou ,&nbsp;Longtao Wang ,&nbsp;Jiayu Wan","doi":"10.1016/j.aca.2026.345116","DOIUrl":"10.1016/j.aca.2026.345116","url":null,"abstract":"<div><h3>Background</h3><div>The global dissemination of monkeypox virus (MPXV) poses a formidable challenge to public health systems worldwide, disrupting surveillance and containment strategies. This crisis underscores the urgent demand for rapid diagnostic technologies that balance high sensitivity for detecting low viral loads and strict specificity to prevent cross-reactivity. To address this unmet need, the present study developed a cascade signal amplification system—named “Monkeypox Fluorescent T4-Ligase Assay (MFTA)”—by integrating recombinase-aided amplification (RAA) and fluorescent RNA aptamer sensing technology, with T4 DNA ligase as the core mediator.</div></div><div><h3>Results</h3><div>MFTA generates single-stranded DNA (ssDNA) templates through 5′-phosphorylated primers combined with Lambda nuclease treatment and employs two specifically designed bifunctional probes: Probe-L, which incorporates a T7 promoter, and Probe-R, which contains a DNA Mango aptamer sequence. In the presence of MPXV, T4 DNA ligase specifically catalyzes probe ligation to form a complete transcription template, further initiating T7 RNA polymerase-mediated in vitro transcription. This process produces abundant RNA Mango aptamers, which specifically bind the TO1 fluorophore to generate a detectable fluorescent signal. The “Identification-Ligation-Transcription-Fluorescence” cascade enables dual verification: RAA ensures target-specific amplification of MPXV sequences, while T4 DNA ligase guarantees precise probe ligation and reduces non-specific background noise. Experiments confirmed MFTA has a detection limit of 1 copy/μL for MPXV, with results consistent with those of qPCR, and effectively discriminates MPXV from other orthopoxviruses.</div></div><div><h3>Significance</h3><div>MFTA innovatively integrates the precise specific recognition of target sequences by T4 DNA ligase with the efficient signal amplification capability of RNA aptamers, successfully establishing a novel technical platform for pathogen detection. Its high sensitivity, strong specificity, and favorable scalability make it a valuable tool for MPXV diagnostics, supporting targeted public health responses to outbreaks and aiding in curbing virus spread.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1389 ","pages":"Article 345116"},"PeriodicalIF":6.0,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145995961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-throughput detection platform for dried blood spots constructed with DESI-MS/MS","authors":"Siqi Gao ,&nbsp;Jinhui Zhao ,&nbsp;Yue Guan ,&nbsp;Liyan Liu","doi":"10.1016/j.aca.2026.345109","DOIUrl":"10.1016/j.aca.2026.345109","url":null,"abstract":"<div><h3>Background</h3><div>Dried blood spot (DBS) technology, as a method of blood collection and preservation, has the advantages of small blood collection, convenient transportation and avoiding the degradation of metabolites. In particular, it has broad clinical application prospects in newborn screening and therapeutic drug monitoring. Desorption electrospray mass spectrometry (DESI-MS) is a simple, rapid and in situ spatial metabolomics detection technique, but its throughput, accuracy and coverage for DBS remain to be investigated compared with traditional methods.</div></div><div><h3>Results</h3><div>In the UPLC-MS/MS platform, the optimal extraction solvent, extraction solvent volume and extraction method were ACN: H₂O (v:v/3:1), 300 μL and ultrasonic extraction, respectively. Based on the DESI-MS/MS platform, the best spray solvent was MeOH: H₂O (v:v/3:1), the spray velocity was set at 2 μL/min, the spray needle angle was set at 55°, and the capillary voltage was set at 4.5 kv (DESI⁺), respectively. The approach delivered consistent data assurance, broad metabolite coverage, and acceptable reproducibility. Finally, these methods were applied to detect the metabolic profiles of subjects before and after drinking sugar-sweetened soy milk, and the metabolic map was well isolated, and the difference metabolites were successfully found.</div></div><div><h3>Significance</h3><div>Two protocols of metabolic profiles for DBS were constructed and optimized. The good performance of DESI-MS/MS was obtained when comparing to the UPLC-MS/MS. The application of sweetened soy milk showed that the two protocols developed have the practicability of detecting differential changes in metabolic profiles of DBS.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1390 ","pages":"Article 345109"},"PeriodicalIF":6.0,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145995957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive soil pollution monitoring system based on convolutional recursive sequence network and terahertz spectroscopy","authors":"Ao Feng ,&nbsp;Lijia Xu ,&nbsp;Yuchao Wang,&nbsp;Zhijun Wu","doi":"10.1016/j.aca.2026.345117","DOIUrl":"10.1016/j.aca.2026.345117","url":null,"abstract":"<div><h3>Background</h3><div>Accurate analysis of the total amount of heavy metals and their distribution patterns in soil can aid in determining the extent of soil pollution and iake appropriate remediation measures.Traditional detection methods are inefficient, and standard machine learning approaches are insufficiently sensitive in detecting the range when dealing with several contaminants of heavy metals. In this study, the contents of three heavy metals, cadmium (Cd), nickel (Ni), and zinc (Zn), in soils of different pollution types and concentrations were detected through terahertz spectral curve analysis.</div></div><div><h3>Results</h3><div>A convolutional recursive sequence network (CRST) with cascaded convolutional kernel and cyclic sequence structure was designed to further improve the soil heavy metal detection accuracy by capturing and integrating the sequence dependencies in the continuous terahertz bands. We compared common machine learning models with feature extraction algorithms, and the experimental results show that the CRST method can accurately identify soil heavy metal contamination species under the combined model of all data. The CARS-CRST combination achieved a classification accuracy of 99.54 % when the soil was contaminated with a single heavy metal. The CARS-CRST combination achieved a classification accuracy of 95.26 % when the soil was contaminated with a mixture of heavy metals. The CRST method can also accurately determine the heavy metal content in soil for Cd, Ni, and Zn, achieving optimal R² and RMSE values when the prediction model is constructed for these elements.</div></div><div><h3>Significance</h3><div>Based on terahertz spectroscopy and machine learning technology, this study conducted both qualitative and quantitative research on soil heavy metals, providing robust technical support for the scientific management and precise monitoring of soil.The results show that terahertz technology and machine learning technology can bring new ideas and prospects for soil pollution detection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1389 ","pages":"Article 345117"},"PeriodicalIF":6.0,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145993140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive chemiluminescence immunoassay utilizing functionalized magnetic metal-organic framework materials for the detection of CEA and CA199","authors":"Xiaomin Zuo ,&nbsp;Wenxia Chen ,&nbsp;Xianlong Lu ,&nbsp;Rui Yuan ,&nbsp;Shuangyi Hu ,&nbsp;Yangyang Xue ,&nbsp;Wei Chen ,&nbsp;Rui Yang","doi":"10.1016/j.aca.2026.345114","DOIUrl":"10.1016/j.aca.2026.345114","url":null,"abstract":"<div><h3>Background</h3><div>In response to the urgent need for highly sensitive and rapid combined detection of multiple tumor markers for the early diagnosis of colorectal cancer, this work innovatively constructs a magnetically-driven chemiluminescence (CL) immunosensing platform (CoFe₂O₄@ZIF-8/luminol-Au NPs).</div></div><div><h3>Results</h3><div>CoFe₂O₄ NPs were firstly aminated via solvothermal method and lysine modification to provide specific binding sites. ZIF-8 was then grown in situ to form a core-shell structure with high specific surface area for efficient luminol loading. Finally, Au NPs were generated in situ within ZIF-8, simultaneously immobilizing luminol to form composite signal units. This design integrates magnetic separation, confinement effects, and signal amplification, generating an ideal microenvironment for enhanced CL reactions. The label-free CL immunoassay achieves an impressive limit of detection (LOD) of 22 fg/mL for CEA and 5.4 fg/mL for CA199, with broad linear ranges spanning over 5 orders of magnitude. The entire detection process is completed within 30 min, and the analysis of real serum samples demonstrates good recoveries.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1389 ","pages":"Article 345114"},"PeriodicalIF":6.0,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145995955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific lysine guanidination and long alkyl chain tagging-assisted negative enrichment strategy enables comprehensive profiling of protein N-terminal acetylome","authors":"Yang Li ,&nbsp;Yu He ,&nbsp;Weijie Zhang ,&nbsp;Mengqing Yang ,&nbsp;Wei Yu ,&nbsp;Zhenbin Zhang","doi":"10.1016/j.aca.2026.345112","DOIUrl":"10.1016/j.aca.2026.345112","url":null,"abstract":"<div><h3>Background</h3><div>Protein N-terminal acetylation (Nt-acetylation) is a widespread and essential modification in both eukaryotes and prokaryotes. To achieve in-depth profiling of Nt-acetylome, enrichment of Nt-acetylated peptides prior to mass spectrometry (MS) analysis is crucial, as their inherently low ionization efficiency is further suppressed in complex peptide mixtures. However, current methods are constrained by low enrichment selectivity and insufficient proteolytic digestion efficiency toward Nt-acetylated peptides.</div></div><div><h3>Results</h3><div>Herein, we present a novel and effective enrichment method involving the specific blocking of lysine ε-amino groups by guanidination and long alkyl chain tagging to N-terminal/internal peptides with free α-amino groups. Due to their enhanced hydrophobicity, the alkylated N-terminal/internal peptides could be efficiently depleted via a C18 column, thus achieving the negative enrichment of Nt-acetylated peptides. Specific guanidination of lysine residues ensures high enrichment selectivity and efficient tryptic digestion of Nt-acetylated peptides. In a single-shot analysis of HeLa cells, our method yielded a significant 4-fold increase in the identification number of Nt-acetylated peptides compared to direct analysis. When coupled with high-pH C18 fractionation, this approach identified 4957 unique Nt-acetylated peptides, corresponding to 3042 acetylated N-termini and 902 putatively neo-acetylated N-termini, markedly expanding the identification coverage of current Nt-acetylation dataset. Furthermore, this method was successfully applied to the quantification of N-terminal acetylome in hippocampal tissues from Alzheimer's disease (AD) mice modeled by amyloid-beta (Aβ) hippocampal injection.</div></div><div><h3>Significance</h3><div>Our method exhibits high enrichment selectivity, efficient proteolytic efficiency and minimal bias toward Nt-acetylated peptides in complex samples. This robust and versatile strategy offers an efficient alternative for comprehensive profiling of Nt-acetylome, thereby facilitating deeper insights into the physiological and pathological functions of Nt-acetylation across diverse biological systems.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1388 ","pages":"Article 345112"},"PeriodicalIF":6.0,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-exosome-membrane DNA polymerization-powered magneto-electrochemical aptasensing for osteosarcoma","authors":"Guang-Xian Zhong ,&nbsp;Huang-Feng Lin ,&nbsp;Fei-Huan Fu ,&nbsp;Jing-Wei Zhou ,&nbsp;Chong-Yu Chen ,&nbsp;Chao-Yang Wu ,&nbsp;Jian-Ping Lin ,&nbsp;Xiong-Wei Xu ,&nbsp;Jian-Hua Lin ,&nbsp;Jin-Yuan Chen","doi":"10.1016/j.aca.2026.345093","DOIUrl":"10.1016/j.aca.2026.345093","url":null,"abstract":"<div><h3>Background</h3><div>The development of exosomes-based non-invasive liquid biopsy method is essential for an early diagnosis of cancers such as osteosarcoma (OS). The emerging isothermal nucleic acid amplification (NAA)-integrated electrochemical aptasensor (E-aptasensor) holds a great promise due to its affordability, rapid response, high sensitivity, and multiplexing capability. However, these aptasensors often require a challenging and complicated integration of signal trigger sequences into the parent aptamers that regulates the NAA. Thus, it is highly desirable to develop a template-free and universal isothermal NAA-intergrated E-aptasensor for OS-derived exosome detection.</div></div><div><h3>Results</h3><div>Herein, via terminal deoxynucleotidyl transferase (TdTase)-mediated DNA polymerization on exosome membrane, we firstly proposed a membrane-initiated enzymatic polymerization (MIEP)-based magneto-driven E-aptasensor for an ultrasensitive detection of OS cell-derived exosomes. Specifically, the LC09 aptamers-modified magnetic microbeads (MMBs) efficiently captured the specific exosomes. Afterwards, the direct on-exosome membrane DNA polymerization was performed by TdTase and then large amounts of horseradish peroxidases were bound via immunoreaction, leading to a strong catalytic current in the substrate of 3,3′,5,5′-tetramethylbenzidine/hydrogen peroxide on the surface of a magnetic glassy carbon electrode. This MIEP-based E-aptasensor reported a satisfactory detection result, displaying a broad linear range (6 × 10⁷∼6 × 10¹⁰ particles/mL), a low detection limit (60 particles/μL), a good specificity toward other tumor cell-derived exosomes, and a good recovery (87.9 %–98.3 % in human plasma). Finally, this method was applied to different clinical samples of micro-volume plasma and distinguished them successfully.</div></div><div><h3>Significance</h3><div>The proposed aptasensor can serve as a powerful and potential tool for liquid biopsy of OS, owing to its high generality, low sample demand, and high practicability. Furthermore, the innovative design strategy established in this work provides a new and versatile avenue for the construction of advanced exosome biosensors, thereby broadening their application in clinical research and precision medicine.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1390 ","pages":"Article 345093"},"PeriodicalIF":6.0,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145993296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}