Analytica Chimica Acta最新文献

{"title":"Atomization of antimony hydride and in-situ preconcentration of antimony in a dielectric barrier discharge atomizer: A mechanistic study by laser induced fluorescence","authors":"Waseem Khan ,&nbsp;Pavel Dvořák ,&nbsp;Martina Mrkvičková ,&nbsp;Milan Svoboda ,&nbsp;Jan Kratzer","doi":"10.1016/j.aca.2025.343787","DOIUrl":"10.1016/j.aca.2025.343787","url":null,"abstract":"<div><h3>Background</h3><div>Dielectric barrier discharge (DBD) ambient plasma has been recently used as hydride atomizers for atomic absorption spectrometry (AAS). DBD performance in terms of sensitivity has been proven comparable with the most common hydride atomizer, heated quartz tube (QTA), for some analyte hydrides while being significantly worse for the others. Moreover, a simple approach to analyte hydride preconcentration directly in the DBD (in-situ) prior to AAS detection has been reported with preconcentration efficiency significantly analyte-dependent. Detailed insights into the mechanisms of analyte hydride atomization and preconcentration are thus essential to utilize the full potential of DBD atomizers in analytical routine.</div></div><div><h3>Results</h3><div>Using SbH₃ as a model analyte hydride and laser induced fluorescence (LIF) as a detector, absolute concentration of Sb free atoms was quantified and their spatial distribution in the DBD discharge was visualized. The atomization efficiency of SbH₃ reaches (75 ± 20) % with homogeneous distribution of Sb free atoms in the whole DBD discharge area indicating long life of ground state free Sb atoms. In addition, the mechanisms of in-situ preconcentration of antimony in the DBD were investigated using LIF. The release of preconcentrated antimony from the inner quartz surface of the DBD walls was visualized and temporally resolved formation of free Sb atoms was acquired. Free atoms are firstly observed in the gas phase in the central part of the DBD, where they had been preconcentrated, having the character of a wave traveling towards the atomizer edges within approximately 2 s.</div></div><div><h3>Significance</h3><div>Both, high atomization efficiency and long life of free Sb atoms found by LIF prove perfect compatibility of DBD atomizer with AAS detection. This agrees well with high sensitivity reached in DBD atomizer in AAS which is comparable to that achieved in QTA. In preconcentration mode, spatio-temporally resolved LIF measurements revealed analyte trapping in a narrow spot in the central part of the DBD and enabled to study the dynamics of its subsequent release.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1347 ","pages":"Article 343787"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo design of dual-detection fluorescent sensors for bisulfite and copper (II) ion based on cascade activation","authors":"Tian Tan ,&nbsp;Wenyu Xi ,&nbsp;Chenxu Duan ,&nbsp;Ying Han ,&nbsp;Peikun Xiao ,&nbsp;Hongqi Yin ,&nbsp;Hong Huang ,&nbsp;Xulang Chen ,&nbsp;Yanjun Hu ,&nbsp;Xiaohuan Huang","doi":"10.1016/j.aca.2025.343789","DOIUrl":"10.1016/j.aca.2025.343789","url":null,"abstract":"<div><h3>Background</h3><div>Cascade activated probes have received extensive attention due to their sequence-dependent detection of multiple markers in pathologic pathways. HSO₃⁻ and Cu²⁺ are biomarkers of intracellular mitochondrial activity, and their simultaneous detection is of great significance. Until now, probes that detect them simultaneously were all based on displacement mechanism with “ON-OFF-ON” switches in an emission channel. In other words, using HSO₃⁻ and Cu²⁺ as two inputs in logic sensing, the inputs of (0,0), (0,1), or (1,1) gave the same “1” output. Therefore, they suffered from the false positive signals in clinical application.</div></div><div><h3>Results</h3><div>The de novo design of fluorescent probes with a more advanced sensing mechanism was explored. The four dyes, <strong>CM-Cu</strong>, <strong>CMA-Cu</strong>, <strong>R1</strong>, and <strong>R2</strong>, have similar structures, but their sensing behaviors are quite different. For dual detection of HSO₃⁻ and Cu²⁺, <strong>CM-Cu</strong> showed emission “ON-OFF-ON” switches based on IMP logic gate; while <strong>CMA-Cu</strong>, having one more aldehyde, was cascade activated based on AND gate for the first time. Noteworthily, unlike most dual-locked probes, which usually involve a complex synthesis process, here, cascade activation by HSO₃⁻ and Cu²⁺ is achieved by adjusting the electron distribution of a ready-made structure. Coupled with its low toxicity, high specificity, strong solid emission, and anti-diffusion ability, the sensing property of <strong>CMA-Cu</strong> have been validated in digital encryption and cell imaging.</div></div><div><h3>Significance</h3><div>We exhibited how to adjust the dual recognition from displacement to cascade activation by fine-tuning the molecular structure. This study may provide a new idea for the simple design of dual-locked probe, which is helpful for the sequential detection of multiple biomarkers with physiological correlation and could improve the accuracy of disease diagnosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1346 ","pages":"Article 343789"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and in-situ detection of iodine and potassium ferrocyanide in table salt using enhanced-Multipass cavity Raman scattering","authors":"Baokun Huang ,&nbsp;Chuqiu Zhao ,&nbsp;Dapeng Yang ,&nbsp;Lin Zhu ,&nbsp;Yunhong Zhang ,&nbsp;Qishen Huang","doi":"10.1016/j.aca.2025.343784","DOIUrl":"10.1016/j.aca.2025.343784","url":null,"abstract":"<div><div>The excess of iodine (I₂) and potassium ferrocyanide (K₄[Fe(CN)₆]) to table salt are harmful to both human health and environment. To date, there are still a lack of suitable methods for simple, rapid and in-situ to detect the I₂ and K₄[Fe(CN)₆] contents in table salt. Herein, we employ a highly sensitive Raman spectroscopy, which is based on multiple reflection cavities and multi-directional signal collection optical systems, to detect the concentration of trace ions in table salt. The detection limit of our technology is less than 1 mg/kg in salt solutions, signifying that the corresponding quantitative detection limit in table salt is 3.45 mg/kg. Simultaneously, the relative Raman intensity of the ion exhibits a highly linear correlation with concentration (R² = 0.999), rendering it a molecular probe for I₂ and K₄[Fe(CN)₆] within table salt. The accuracy of this molecular probe for I₂, K₄[Fe(CN)₆], and sulfate (SO₄²⁻) content in different salts is 87.2 %, 89.6 % and 95.6 % respectively.Compared with the traditional method, this method has the advantages of low sample consumption (1g of salt), fast detection speed (10 min), and the measured sample can be saved.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1347 ","pages":"Article 343784"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selectivity modulation and its prediction in cyclofructan-6 phase for chaotropic ions","authors":"Siddharth Jaya Sajeevan J,&nbsp;M. Farooq Wahab,&nbsp;Saba Aslani,&nbsp;Daniel W. Armstrong","doi":"10.1016/j.aca.2025.343788","DOIUrl":"10.1016/j.aca.2025.343788","url":null,"abstract":"<div><h3>Background</h3><div>Modifying additives to alter the selectivity of a single stationary phase and solvent system is appealing in mixed-mode separations. Silica-bonded cyclofructan-6 uniquely binds with cations strongly in the presence of organic solvents and is hydrolytically stable. Perchlorate with inorganic and organic quaternary ammonium cations could adjust retention for molecules with carboxylic acids, sulfonic acids, and nitrogen-containing functional groups, which are common in pharmaceutically relevant molecules. Perchlorate salts of ammonium, lithium, tetrabutylammonium, and tetramethylammonium were assessed for their selectivity in acetonitrile-water eluent systems.</div></div><div><h3>Results</h3><div>This study provides a mathematical route to accomplish complex selectivity alterations by simply varying the perchlorate counterions. Two predictive tests based on <span><math><mrow><msub><mi>l</mi><mn>2</mn></msub></mrow></math></span>-norm and principal component analysis (PCA) were proposed to choose the most distinct perchlorate additive pair for 17 test probes in retention time-space. The <span><math><mrow><msub><mi>l</mi><mn>2</mn></msub></mrow></math></span>-norm provides a faster approach to measure variation caused by eluent additives for probe analytes. Ammonium and tetrabutylammonium perchlorate salts showed the maximum “distance” between them. The eluent additives strongly affected the elution times of carboxylic and sulfonic acids, and neutrals were mildly altered. A concentration study on these two additives with probe analytes ruled out a simple ion exchange interaction and suggested a multimodal retention mechanism. All additives yielded high-efficiency separations expected of 2.7 μm core-shell particles.</div></div><div><h3>Significance</h3><div>The proposed mathematical tests will assist chromatographers in selecting distinct eluent additives for different classes of separations during method development. The applicability of the eluent selection strategy has been shown with the separation of three different biologically important classes of molecules containing analytes like cocaine, amphetamine, tianeptine, serotonin, lorazepam, and 5-fluorouracil.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1347 ","pages":"Article 343788"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel quantum dot-based ratiometric fluorescence sensor array: For reducing substances detection and Baijiu quality discrimination","authors":"Xuhui Xia ,&nbsp;Peng Zhao ,&nbsp;Jia Zheng ,&nbsp;Xuheng Li ,&nbsp;Xin Zeng ,&nbsp;Dianhui Men ,&nbsp;Yiyao Luo ,&nbsp;Changjun Hou ,&nbsp;Danqun Huo","doi":"10.1016/j.aca.2025.343785","DOIUrl":"10.1016/j.aca.2025.343785","url":null,"abstract":"<div><h3>Background</h3><div>Discriminating the quality of baijiu is critical for fostering the growth of the China baijiu market and safeguarding customers' rights. However, establishing a small-scale and rapid baijiu discriminating sensor assay still remains a challenge.</div></div><div><h3>Results</h3><div>Here, we first introduced ratiometric fluorescence sensor array for the detection of reducing substances in baijiu to achieve baijiu discrimination. A ratiometric fluorescence sensor array is built using 2,3-diaminophenazine (oxidized-state OPD, oxOPD) to quench three distinct fluorescence signals of quantum dots while reducing interference from background signals. The reducing chemicals in baijiu can react with Ag⁺, weakening the quenching effect and changing the ratio. The discriminating of 12 types of organic small molecules which were presented in baijiu was achieved with 97.2 % accuracy by using machine learning classification methods. Meanwhile, 0.1 μM limit of detection (LOD) for ascorbic acid shows that our methods have the potential to quantitative detect reducing substances. In real sample detection, our methods can discriminate 10 distinct qualities of baijiu with 100 % accuracy. We also encoded the fingerprints of different varieties of baijiu for quality control and information reading.</div></div><div><h3>Significance and novelty</h3><div>Overall, our easy but robust sensing array not only overcomes the problem of background signal interference but also gives an ideal way for discriminating different qualities of baijiu, food and other areas.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1347 ","pages":"Article 343785"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis of deep learning and chemometric approaches for spectral data modeling","authors":"Rúben Gariso,&nbsp;João P.L. Coutinho,&nbsp;Tiago J. Rato,&nbsp;Marco S. Reis","doi":"10.1016/j.aca.2025.343766","DOIUrl":"10.1016/j.aca.2025.343766","url":null,"abstract":"<div><h3>Background:</h3><div>This study presents a comprehensive comparison of five different modeling approaches for spectroscopic data analysis. The first approach uses PLS combined with classical chemometric pre-processing (9 models). The second and third approaches use interval PLS (iPLS) with either classical pre-processing or wavelet transforms (28 models). The fourth uses LASSO with wavelet transforms (5 models). Finally, the fifth approach combines CNN with spectral pre-processing (9 models).</div></div><div><h3>Results:</h3><div>In this paper we consider two low dimensional case studies: a regression problem for a beer dataset (40 training samples) and a classification problem for a waste lubricant oil dataset (273 training samples). The results show that, after exhaustive pre-processing selection, iPLS variants show better performance for the first case study and remain competitive in the second case study. Wavelet transforms proved to be a viable alternative to classical pre-processing, improving performance for both linear and CNN models while maintaining interpretability. For the second case study, with more data, CNNs present good performance when applied on raw spectra and could potentially be used to avoid exhaustive pre-processing selection. However, it was found that CNNs can benefit from some types of pre-processing, leading to improved performance for the first case study and overall better performance for the second case study.</div><div><strong>Significance and Novelty:</strong></div><div>This study provides a critical and exhaustive comparison of combinations of pre-processing methods and models for spectroscopic data analysis. It was found that no single combination of pre-processing and model that can be identified as optimal beforehand in low data settings.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1347 ","pages":"Article 343766"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid droplet-targeting carbonized polymer dots for intracellular polarity visualization under wash-free conditions","authors":"Dongmei Wang ,&nbsp;Genxiu Yao ,&nbsp;Junyong Sun ,&nbsp;Juanjuan Liang ,&nbsp;Huan Zhou","doi":"10.1016/j.aca.2025.343790","DOIUrl":"10.1016/j.aca.2025.343790","url":null,"abstract":"<div><h3>Background</h3><div>For an extended period, lipid droplets (LDs) have been regarded to be the storage depots for metabolic lipids. As a key to energy storage and regulation, lipid droplets (LDs) have been found to perform crucial functions in various physiological processes such as oxidative stress and cell migration. Abnormal polarity variations of LDs are associated with various pathological conditions, including lipid metabolism disorders, the development of atherosclerosis and cancer. Therefore, developing LD-targeting polarity-sensitive probes and effectively monitoring the variations in the LDs microenvironment is highly meaningful.</div></div><div><h3>Results</h3><div>A highly specific LD-targeting carbonized polymer dot (LD-CPD)-based fluorescent probe with amphiphilicity was designed and synthesized under mild conditions. The new probe exhibits high polarity-sensitivity, low viscosity crosstalk, strong solvatochromic and high photobleaching resistance. The solvatochromic responses of LD-CPD are attributed to hydrogen bonding and solvent dipole-dipole interactions, with hydrogen bonding exerting a stronger influence. In addition, it demonstrates a turn-off response to water, which makes it effective for LDs washing-free imaging. Moreover, as a solvatochromic probe, the proposed CPD can well achieve ratiometric imaging of subtle polarity changes within LDs during starvation and oxidative stress, indicating its capability to observe physiological dynamic changes.</div></div><div><h3>Significance</h3><div>All the features remind that the developed LD-CPD probe has great potential for diagnosis in LDs monitoring and analysis. It will serve as a robust monitoring platform for identifying diseases associated with abnormal LDs polarity. It also expands the application of CPDs in bioanalysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1346 ","pages":"Article 343790"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “A novel interval sparse evolutionary algorithm for efficient spectral variable selection” [Anal. Chim. Acta 1341 (2025) 343655]","authors":"Mingrui Li,&nbsp;Yonggang Li,&nbsp;Chunhua Yang,&nbsp;Hongqiu Zhu,&nbsp;Can Zhou","doi":"10.1016/j.aca.2025.343739","DOIUrl":"10.1016/j.aca.2025.343739","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1346 ","pages":"Article 343739"},"PeriodicalIF":5.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-activated aptasensor for plug-and-play detection of Aflatoxin B1 in food samples","authors":"Yongmei Jia ,&nbsp;Bing Han ,&nbsp;Guohua Zhou ,&nbsp;Zhiguo Li ,&nbsp;Mingyue Xue ,&nbsp;Bo Liang ,&nbsp;Peilian Liu ,&nbsp;Yong Cheng","doi":"10.1016/j.aca.2025.343786","DOIUrl":"10.1016/j.aca.2025.343786","url":null,"abstract":"<div><h3>Background</h3><div>AFB1 contamination in food samples is a global concern, threatening food safety and human health. Numerous assays have been developed for AFB1 detection. Among them, fluorescent aptasensors are of great interest due to their simplicity of operation, easy to read signal output. Nevertheless, these aptasensors are “passive-type”, means the target AFB1 bind with the aptamer directly and cause fluorescent signal response. They are “always active”, may result in undesired signal generation and the process is hard to control. Generally, the “activable-type” aptasensor would avoid these defects.</div></div><div><h3>Results</h3><div>Here we demonstrate a light activated aptasensor for plug-and-play detection of AFB1. The aptasensor is constructed by integrating an AFB1-specific aptamer with a complementary DNA strand (PC-strand) that incorporates a photocleavable o-nitrobenzyl group. The AFB1 aptamer is locked by PC-strand preventing it binding to target AFB1. With ultraviolet (UV) light irradiation, the o-nitrobenzyl group cleaves and releases the PC-strand to two short DNA fragments. This fragmentation reduces the hybridization stability with AFB1 aptamer, allowing target AFB1 to bind efficiently to AFB1 aptamer and accompanied by increasing in fluorescence signal. The aptasensor could be activated at desired time, provides a sensitive (0.074 ng/mL) and specific method for AFB1 detection. It has been demonstrated to be a reliable tool for the analysis of AFB1 in food samples (rice, corn, and soybean), yielding satisfactory results.</div></div><div><h3>Significance and novelty</h3><div>The aptasensor is insert to the target AFB1, while its activity could be restored in a time-resolved manner by cleaving the PC-strand to two short strands using UV light irradiation. This approach provides a promising platform for the rapid screening of AFB1 contamination in food samples, contributing to food safety and quality control.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1346 ","pages":"Article 343786"},"PeriodicalIF":5.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design of a near-infrared fluorescent probe for rapid monitoring of carboxylesterase in live cells and drug-induced liver injury mice","authors":"Weitao Xing ,&nbsp;Kaili Yang ,&nbsp;Yonglong Zhu ,&nbsp;Xinyi Li ,&nbsp;Yan Zhang ,&nbsp;Linxia Guo ,&nbsp;Jing-Yuan Ge ,&nbsp;Yang Bai ,&nbsp;Zhongyan Chen","doi":"10.1016/j.aca.2025.343782","DOIUrl":"10.1016/j.aca.2025.343782","url":null,"abstract":"<div><h3>Background</h3><div>Carboxylesterase (CE) is an important enzyme that mainly exists in liver cells and can catalyze the hydrolysis of esters in a variety of pharmaceuticals and xenobiotics. Real-time and non-invasive imaging of CE is of great significance for the study of CE-related metabolic diseases. Although fluorescence sensing technology is considered a promising candidate, the slow response rate (&gt; 60 min), low sensitivity, and short emission wavelength (&lt;650 nm) of most CE probes limit their practical application. Therefore, it is significant and urgent to develop novel fluorescent probes for the rapid diagnosis of CE-related diseases.</div></div><div><h3>Results</h3><div>Herein, a near-infrared fluorescent probe, <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong>, has been developed by introducing acetyl as the CE recognition unit into the fluorophore <em>meso</em>-trifluoromethyl-BODIP for the detection of CE. <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong> exhibited a remarkable fluorescence enhancement at 690 nm for CE with a limit of detection of 7.9 × 10⁻⁴ U/mL. Importantly, the fast response kinetics (within 3 min) make <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong> superior to most reported probes. The emission turn-on mechanism was confirmed by theoretical calculation, revealing that after the hydrolysis of <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong>, the intramolecular charge transfer process leads to strong fluorescence. Furthermore, <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong> has been successfully applied to real-time imaging of endogenous CE changes in living cells and to imaging CE activity differences between tumor and normal cells. In addition, <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong> has been successfully used to track CE abnormalities in acetaminophen-induced liver injury model mice.</div></div><div><h3>Significance</h3><div>A NIR fluorescent probe <strong>CF</strong>_{<strong>3</strong>}<strong>-BDP-CE</strong> was developed to effectively <u>track</u> the dynamic change of CE fluctuation in living cells and mice, with potential applications in the diagnosis of CE-related diseases.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1346 ","pages":"Article 343782"},"PeriodicalIF":5.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}