Analytica Chimica Acta最新文献

{"title":"A mitochondria-targeted nitric oxide probe with large Stokes shift for real-time imaging and evaluation of inflammatory bowel disease in situ","authors":"Jiatian Liu ,&nbsp;Xueqian Chen ,&nbsp;Andong Wang ,&nbsp;Dongdong Su","doi":"10.1016/j.aca.2024.343372","DOIUrl":"10.1016/j.aca.2024.343372","url":null,"abstract":"<div><h3>Background</h3><div>Inflammatory bowel disease (IBD) is a prevalent inflammatory disorder, and the abnormal expression of nitric oxide (NO) produced by biocatalysis of iNOS enzyme in mitochondria is directly associated with the occurrence and progression of IBD. Activatable fluorescent probes offer promising tools for early diagnosis of IBD, however, inadequate biodistribution and limited targeting properties of these probes <em>in vivo</em> severely impede accurate diagnosis of IBD and real-time evaluation of inflammatory levels <em>in situ</em>. Therefore, it is necessary to design a highly efficient fluorescent probe towards NO to overcome inadequate biodistribution and achieve accurate diagnosis and evaluation of IBD <em>in situ</em>.</div></div><div><h3>Results</h3><div>We designed a highly efficient mitochondria-targeted “turn-on” NIR fluorescent probe <strong>Cy-OMe</strong> which has excellent targeting properties and imaging ability. The response mechanism is probe <strong>Cy-OMe</strong> rapidly undergoes N-nitrosation reaction resulting in “turn-on” NIR fluorescence signal when exposed to NO. <strong>Cy-OMe</strong> exhibits high sensitivity and specificity in detecting NO content <em>in vitro</em>, owing to its large Stokes shift. Furthermore, the probe <strong>Cy-OMe</strong> not only efficiently targets mitochondria but also enables precise assessment of fluctuations in endogenous NO concertation across various cell types. Importantly, by virtue of large Stokes shift and excellent mitochondrial targeting ability, <strong>Cy-OMe</strong> has the capability to specifically evaluate dynamic fluctuations of NO in lipopolysaccharide (LPS)‐stimulated IBD mouse models <em>in situ</em> and <strong>Cy-OMe</strong> was achieved high-contrast imaging and precision diagnosis of intestinal inflammation diseases.</div></div><div><h3>Significance</h3><div><strong>Cy-OMe</strong> can accurately assess fluctuations in NO levels and show high signal fidelity in the diseased intestine region, which has prospects in the non-invasive diagnosis of intestinal inflammation <em>in vivo</em>. At the same time, it is expected to serve as a potential diagnose platform for investigating the physiological processes underlying NO-related inflammatory diseases and promoting understanding of the pathological functions of NO across diverse inflammatory diseases.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343372"},"PeriodicalIF":5.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of chemosensitivity of tumor spheroids exposed to two-dimensional gradient of combination drugs in a hydrogel-based diffusion microfluidic platform","authors":"Kin Fong Lei ,&nbsp;Yu-Ting Lin ,&nbsp;Sai Kiran Reddy Boreddy ,&nbsp;Ping-Ching Pai","doi":"10.1016/j.aca.2024.343371","DOIUrl":"10.1016/j.aca.2024.343371","url":null,"abstract":"<div><h3>Background</h3><div>Liver cancer stands as a leading cause of cancer-related deaths globally, challenging conventional treatments due to resistance to chemotherapy and targeted therapy. Although frontline medications show initial efficacy, prolonged use often leads to resistance and harm. Current clinical strategies rely on combination therapies, but evaluating their effectiveness remains challenging.</div></div><div><h3>Results</h3><div>To address this, we developed a hydrogel-based diffusion microfluidic platform for assessing chemosensitivity. This platform features a hydrogel-filled diffusion layer linked to liquid wells, allowing the creation of drug gradients. Tumor spheroids, cultured on the non-adhesive hydrogel surface, were exposed to single or combination drug gradients. Analysis revealed that drug efficacy, quantified by IC₅₀ values, could be determined from responses to single drug gradients. A novel method was introduced to assess spheroid circularity as a time-invariant index of drug efficacy. Furthermore, exposing spheroids to 2D combination drug gradients allowed intuitive visualization of their responses via a color map. This analysis identified optimal drug combinations, exhibiting superior efficacy to monotherapy.</div></div><div><h3>Significance</h3><div>The microfluidic platform enables assessment of synergistic effects and replicates <em>in vivo</em> conditions, enhancing the relevance of test results. By offering a streamlined, fast, and efficient drug screening approach, this platform aims to provide insight into tumor spheroid responses to varying drug combinations, facilitating more effective clinical applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343371"},"PeriodicalIF":5.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142488684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of purity of human albumin preparations for clinical use","authors":"Liye Maeyama,&nbsp;Stefanie Fas,&nbsp;Jörg Schüttrumpf,&nbsp;Selma Henrichsen","doi":"10.1016/j.aca.2024.343364","DOIUrl":"10.1016/j.aca.2024.343364","url":null,"abstract":"<div><h3>Background</h3><div>Albumin is the most prevalent plasma protein and serves numerous physiological roles, both in body fluid management and in various other capacities. In many diseases, a deficiency of albumin has been observed, and in certain conditions, albumin substitution has been demonstrated to improve outcome in comparison to plasma expansion using crystalloid or other colloid solutions. The favourable effects of using albumin in patients with liver cirrhosis are likely associated with the non-oncotic functions of albumin. Albumin for clinical use is obtained through fractionation of pooled donor plasma. The production procedures are optimized to ensure pure, chemically uncompromised and native protein.</div></div><div><h3>Results</h3><div>We have extensively analysed commercial preparations of human albumin for clinical use from six different providers. Parameters that must correspond to the requirements of international pharmacopoeias were assessed (aluminium, ethanol, sodium, the presence of dimers and oligomers) and found to conform in all cases. In addition, we used for the first time nuclear magnetic resonance (NMR) as an additional analytical approach for investigating in greater depth the quality of a biological remedy gained from human plasma. We applied both ¹H NMR and ¹³C-HSQC for confirming the identity of the albumin preparations, which also conformed in all cases. Moreover, we utilized T₂-filtered ¹H NMR and ¹³C-HSQC measurements to identify the presence of small molecules in the preparations. This demonstrated similar patterns of additional substances present, but also unveiled certain differences in purity in the products of the different providers.</div></div><div><h3>Significance</h3><div>Our analyses confirmed that albumin preparations in clinical use conform to the requirements. We furthermore demonstrate that NMR measurements can provide further depth in identity and purity measurements of biologicals. Despite largely standardized protocols in pharmaceutical albumin production, our in-depth analyses revealed differences in purity. Some samples exhibited lower levels of components other than albumin. We discuss possible causes of these observations and their potential implications for clinical therapy.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343364"},"PeriodicalIF":5.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced design of target-driven self-powered sensor assisted by cascade catalytic strategy","authors":"Zongshan Zhang ,&nbsp;Jing Xu ,&nbsp;Lei Zhang ,&nbsp;Gaoli Zhang ,&nbsp;Hui Li","doi":"10.1016/j.aca.2024.343359","DOIUrl":"10.1016/j.aca.2024.343359","url":null,"abstract":"<div><div>In this work, a self-powered microsensor platform based on enzyme biofuel cells (EBFCs) was developed for intelligent monitoring of disease markers miRNA-451. The cascade catalysis system constructed by using the strategy of enzyme-like ZIF-8 nanocapsule incorporation with biological enzymes, which could simultaneously take into account the specificity of biological enzymes and the high activity of nano-enzymes, significantly promoted the electron transfer between glucose and the bio-anode surface, and improved the sensitivity and stability of the sensing system. Meanwhile, the target-triggered hybridization chain reaction (HCR) amplification strategy to achieve exponential signal amplification based on accurate recognition, and jointly improve the detection sensitivity. As expected, the micro-sensor platform has a wide linear range of 0.5–1.0 fmol/L with a low limit of detection (LOD) of 0.13 fmol/L (S/N = 3) and exhibits excellent selectivity, reproducibility and stability in interference assays under optimal detection conditions. The designed self-powered system is simple to construct, easy to transport and the data transmission mode is intelligent and controllable, which is expected to be used in basic biochemical research, clinical diagnosis and environmental monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343359"},"PeriodicalIF":5.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive targeted profiling of multiple steroid classes in rodent plasma using liquid chromatography-mass spectrometry","authors":"Taťána Gazárková ,&nbsp;Hana Kočová Vlčková ,&nbsp;Kateřina Plachká ,&nbsp;Karla Vagnerová ,&nbsp;Dominika Dubecová ,&nbsp;Petra Klusoňová ,&nbsp;Jiří Pácha ,&nbsp;Frantisek Svec ,&nbsp;Lucie Nováková","doi":"10.1016/j.aca.2024.343362","DOIUrl":"10.1016/j.aca.2024.343362","url":null,"abstract":"<div><h3>Background</h3><div>Reliable quantification of multiple steroid classes in biological fluids within a single method remains an analytical challenge despite many previously published methods. Crosstalk of positional isomers, overlap of stereoisomer fragmentation patterns, differing proton affinities, in-source fragmentation, varying stability of protonated ions in the gas phase across steroid classes, and non-existence of steroid-free matrix are the main challenges limiting the number of simultaneously profiled steroids.</div></div><div><h3>Results</h3><div>In this study, we focused on the development of a derivatization-free, achiral, high-throughput, and cost-effective UHPLC-MS/MS approach that allows simultaneous profiling of a spectrum of 38 steroids covering progestogens, androgens, corticosteroids, and estrogens, while properly addressing the hurdles of steroid analysis. Within a 20-min method, 16 stereoisomers and 15 positional isomers were fully resolved within a single run while separated from 7 additional non-interfering steroids and matrix interferences in rodent plasma. Protein precipitation (PP) and supported liquid extraction (SLE) methods using only 40 μL of sample were developed to achieve the lowest possible limits of quantification. Nevertheless, 5α-dihydroprogesterone and 3α,5α-THDOC could be only qualitatively assessed when using PP. In contrast, DHEA-S could not be quantified or identified when using SLE. A novel surrogate matrix-background subtraction approach, using rat plasma after the animal's adrenalectomy, has been implemented into the optimized PP-UHPLC-MS/MS workflow, successfully validated according to the unified ICH/EMA M10 guidelines, and compared to the traditional quantification strategies. Moreover, the validity of the newly adopted approach has been verified by the targeted profiling of multiple biologically active endogenous steroids in more than 500 samples of mouse plasma in total.</div></div><div><h3>Significance</h3><div>Underestimation of hurdles associated with steroid analysis often compromises the accurate steroid quantification. Our comprehensive, fully validated UHPLC-MS/MS method targeting a wide spectrum of endogenous steroids, mitigating steroid crosstalk and using a minimal sample volume together with a novel surrogate matrix-background subtraction approach significantly advances steroid analysis for research and clinical applications covering multiple biological scopes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343362"},"PeriodicalIF":5.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode sensing platform for Bisphenol A detection via bifunctional CsPbBr3@Cu-MOF assisted fluorescence and colorimetric analysis","authors":"Junyi Ma ,&nbsp;Ying-zhuo Shen ,&nbsp;Jianxiao Gong ,&nbsp;Seitkhan Azat ,&nbsp;Zheng Ping Ji ,&nbsp;Xiao-Ya Hu ,&nbsp;Qin Xu","doi":"10.1016/j.aca.2024.343354","DOIUrl":"10.1016/j.aca.2024.343354","url":null,"abstract":"<div><h3>Background</h3><div>Bisphenol A (BPA) has been identified as an endocrine disruptor with numerous detrimental effects on human health. There is an urgent need to develop fluorescence/colorimetric dual-mode sensing approaches with expanded detection linear range, increased accuracy, and enhanced application flexibility for BPA detection. The utilization of fluorescence and colorimetric signals in point-of-care applications and real-time sensitive sensing further highlights the significance of developing novel and efficient fluorescence/colorimetric dual-mode sensing platform with high-efficiency probes.</div></div><div><h3>Results</h3><div>Herein, a fluorescence and colorimetric dual-mode aptasensor was developed by using CsPbBr₃@Cu-MOF as the aptamer immobilization matrix and signal generator. CsPbBr₃ was functionalized with Cu-MOF using a simple two-step strategy. This strategy involved in-situ formation and modified ligand-assisted precipitation technique with 4,4′-bipyridine (4,4-Bpy) serving as the bifunctional linker. The resulting CsPbBr₃@Cu-MOF exhibited improved stability in water and enhanced fluorescence. Additionally, it functioned as peroxidase mimetic to oxidize 3,3′,5,5′-tetramethyl benzidine (TMB), leading to a colorimetric change from colorless to blue. In the presence of BPA, aptamers were removed from CsPbBr₃@Cu-MOF. Consequently, the fluorescence and peroxidase-activity of CsPbBr₃@Cu-MOF were recovered, resulting in the enhanced fluorescence intensity and color change of TMB. Using this system, the proposed aptasensor demonstrated detection ranges of 1.0–80.0 nM with a LOD of 0.60 nM for the colorimetric method, and a linearity range of 0.1–100 nM with a LOD of 0.02 nM for the fluorescence method.</div></div><div><h3>Significance</h3><div>The obtained CsPbBr₃@Cu-MOF composites showed excellent fluorescence properties, good peroxidase-like activity, and aqueous stability. Furthermore, the proposed dual-mode aptasensor demonstrated simplicity, cost-effectiveness and good anti-interference abilities. This can be extended to the construction of other dual-mode sensors by changing aptamers and provides novel insights on the potential applications of perovskites in bioanalysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343354"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced data point importance: Layered significance of variables in multivariate calibration","authors":"Somaye Vali Zade ,&nbsp;Klaus Neymeyr ,&nbsp;Mathias Sawall ,&nbsp;Hamid Abdollahi","doi":"10.1016/j.aca.2024.343357","DOIUrl":"10.1016/j.aca.2024.343357","url":null,"abstract":"<div><h3>Background</h3><div>The Enhanced Data Point Importance (EDPI) method, a systematic approach for evaluating the importance of data points in multivariate calibration, is introduced. Factor decomposition methods allow for the evaluation of the impact of variables on maintaining the structural pattern of data in the abstract space. Essential data points play a key role in these patterns and the method of Data Point Importance (DPI) aims to evaluate the essential data points in terms of their importance. All other points are rated by zero. In this contribution, DPI is extended to include inner points to evaluate the importance of these points in the absence of the essential points. The EDPI method employs convex peeling to sort data points systematically.</div></div><div><h3>Results</h3><div>EDPI method was applied to near-infrared and Raman spectroscopy data sets, including corn and alcohol mixtures and simulated data, to rank and select important variables. EDPI effectively identified variables that contributed to the preservation of the data structure and highlighted key spectral regions with different degrees of selectivity. In the alcohol dataset, EDPI revealed important physicochemical insights by focusing on specific regions where non-analytes spectra overlapped. It performed in a similar way to the Variable Importance in Projection (VIP) method, but with fewer variables selected.</div></div><div><h3>Significance</h3><div>The experimental results obtained from calibrating near-infrared and Raman spectroscopic datasets using partial least squares highlight the effectiveness of the proposed EDPI strategy when contrasted with the conventional variable importance in projection (VIP) method for variable selection.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343357"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-The-Flight trapping, LIBS analysis and discrimination of single meteorite particles generated by laser ablation","authors":"C. Burgos-Palop ,&nbsp;F.J. Fortes ,&nbsp;P. Purohit ,&nbsp;T. Delgado ,&nbsp;J. Laserna","doi":"10.1016/j.aca.2024.343361","DOIUrl":"10.1016/j.aca.2024.343361","url":null,"abstract":"<div><h3>Background</h3><div>Thousands of micrometeorites fall to the Earth on a daily basis. Most of these meteorites have a rocky composition, but others are mainly composed of iron and nickel. Due to their small size, often ca. 100 μm in diameter, the process of searching for, collecting, and identifying these samples is remarkably tedious. In this work, we introduce a minimally invasive methodology for evaluating the full elemental composition of micrometeorites using optical emission spectroscopy of single particles produced by laser ablation of bulk targets.</div></div><div><h3>Results</h3><div>Bulk meteorite samples were directly ablated within an ablation cell. From few micrograms of ablated matrix, we originated dry aerosols consisting of multielemental particles which were representative of the sample chemistry. SEM images confirmed that the generated particles exhibited spherical geometry. Particles were first optically trapped in air and, then, analyzed by laser-induced breakdown spectroscopy (LIBS). LIBS spectra evidenced compositional differences among samples. For example, <em>Campo de Cielo</em> meteorite featured a high iron content due to its metallic nature whereas LIBS results for <em>Jbilet Winselwan</em> and <em>NWA 869</em> suggested that pyroxene components dominated the composition of the samples. In contrast, <em>NWA 13739</em> and <em>Vaca Muerta</em> contained high aluminum intensity, thus indicating that the feldspathic component was dominant, as then verified by XRD. The intra-sample compositional variability were quite satisfactory, as revealed by the RSD data, below 45 %. For quantitative analysis, the percentages of FeO, SiO₂, Al₂O₃, Na₂O, MgO, TiO₂, CaO, K₂O, MnO, SrO, Cr₂O₃, and Li₂O were calculated using CF-LIBS.</div></div><div><h3>Significance</h3><div>This work demonstrates the applicability of laser excitation of individual particles in an optical trap for the multielemental analysis of meteorites. The methodology provides a complete overview of the samples, is capable of classificating them according to their main phases and yield preliminary quantitative information about those phases. Therefore, we present a minimally destructive pathway to be used as the first step in the inspection of these delicate samples. If the particles represent nanostructures inherent to the meteorites, it would constitute a major step in the analysis of extraterrestrial material that may provide fundamental insights into the structure and composition of the original materials at the microscale, a topic that remains an active area of research worldwide.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343361"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-level data fusion strategy based on urinary nucleosides SERS spectra and blood CEA levels for enhanced preoperative detection of lymph node metastasis in colorectal cancer","authors":"Jinyong Lin ,&nbsp;Yuduo Wu ,&nbsp;Zhizhong Lin ,&nbsp;Xueliang Lin ,&nbsp;Qiong Wu ,&nbsp;JiaJia Lin ,&nbsp;Yuanji Xu ,&nbsp;Shangyuan Feng ,&nbsp;Junxin Wu","doi":"10.1016/j.aca.2024.343360","DOIUrl":"10.1016/j.aca.2024.343360","url":null,"abstract":"<div><h3>Background</h3><div>Preoperative prediction of lymph node metastasis (LNM) plays a crucial role in the treatment and prognosis of colorectal cancer (CRC). The traditional histopathological examination is invasive and time-consuming, providing pathological features only postoperatively. Preoperative serum carcinoembryonic antigen (CEA) is strongly correlated with postoperative LN status. However, the detection accuracy of LNM based on a single preoperative CEA level is low. Therefore, developing a more powerful and sensitive diagnostic tool would be of great clinical value for improving the accurate preoperative prediction of LNM in CRC patients.</div></div><div><h3>Results</h3><div>This study aimed to develop a mid-level fusion approach using urinary nucleosides Raman spectra and blood CEA data to enhance the preoperative discrimination of CRC patients with and without LNM. Surface-enhanced Raman scattering (SERS) spectra of urinary modified nucleosides, isolated by affinity chromatography, were first acquired from 48 patients with LNM and 49 patients without LNM. The principal component analysis (PCA) scores obtained from the SERS spectra were then combined with preoperative blood CEA values to create a fused data array. The discriminant accuracy based on either dataset alone or the fused data was evaluated using three machine learning algorithms: linear discriminant analysis, k-nearest neighbors, and support vector machine. Results showed that the fused data could discriminate between the two groups with an accuracy of up to 91 %, outperforming SERS alone (86 %) and CEA alone (69 %).</div></div><div><h3>Significance</h3><div>To our knowledge, this is the first report of mid-level data fusion of urinary nucleosides SERS spectra with blood CEA levels for the preoperative prediction of LNM in CRC. This work demonstrates that the mid-level data fusion strategy aided by SVM algorithm can greatly improve the preoperative prediction accuracy of LNM. This is crucial for therapeutic decision-making and prognostic assessment in CRC.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343360"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence imaging of dopamine in living cells triggered by unique coupling reactions","authors":"Shaofei Fu ,&nbsp;Jianwen Qiu ,&nbsp;Biaofeng Gu ,&nbsp;Yong Gao","doi":"10.1016/j.aca.2024.343358","DOIUrl":"10.1016/j.aca.2024.343358","url":null,"abstract":"<div><h3>Background</h3><div>Dopamine (DA) is a significant neurotransmitter and catecholamine molecule in the mammalian brain. It plays a crucial role in perception, cognition, central nervous system regulation, and hormone secretion. The detection of DA levels in living systems is a vital aspect of the research and early diagnosis of neurological disorders.</div></div><div><h3>Results</h3><div>In this study, we achieved an effective fluorescence generation specific to DA under physiological pH conditions by coupling the DA detection reaction to the HClO oxidation reaction. This method has been used for the detection of DA in cells, as well as for the visualization of DA levels in cellular models of inflammation and depression.</div></div><div><h3>Significance</h3><div>This is the first time that utilizes an organic fluorescent molecular probe to detect DA in living cells.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1332 ","pages":"Article 343358"},"PeriodicalIF":5.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}