Analytica Chimica Acta最新文献_第7页

Point-of-care colorimetric biosensor for H2O2 and glucose detection utilizing the peroxidase-like activity of 2D bimetallic metal organic framework nanosheets 利用二维双金属金属有机框架纳米片的过氧化物酶样活性检测 H2O2 和葡萄糖的床旁比色生物传感器

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-04 DOI: 10.1016/j.aca.2025.343993

Yasmeen M. Abdelfattah , Amr M. Mahmoud , Noha I. Abdelaziz , Dina A. El Mously

Background

The applications of natural enzymes are vast, limited only by their protein nature. Therefore, the development of artificial enzyme mimetics, nanozymes, which are stable and have improved activity, has become indispensable for biomedical and diagnostic purposes. Nanozymes have developed into an emergent topic combining nanotechnology and biology due to their vast range of potential uses. In comparison to natural peroxidase, peroxidase-imitating nanozymes have distinct benefits in terms of high stability and low cost for applications in bioanalysis and environmental remediation. The use of metal-organic framework nanoparticles has exhibited enhanced catalytic and enzymatic performance.

Results

In the current work, we present a strategy for synthesizing 2D Ni/Co MOF nanoparticles that have been anchored onto carboxymethyl cellulose (CMC). The resulting composite (Ni/Co-MOF@CMC) 2D nanosheets exhibit a high surface area and abundant catalytic sites, greatly amplifying their peroxidase-like catalytic performance. Additionally, these 2D bimetallic MOFs mimic the peroxidase activity, demonstrated by the distinctive yellow colour upon the oxidation of o-Phenylenediamine (OPD) by hydrogen peroxide. This newly synthesized 2D bimetallic MOF provides a straightforward, simple, selective, and sensitive colorimetric analysis technique for the determination of hydrogen peroxide and glucose. H₂O₂ could be efficiently detected with a linear range of 10 μM–800 μM and a lower detection limit of 3.28 μM. With the potential to detect minute glucose concentrations as low as 200 μM within a linear range of 200 μM–600 μM.

Significance and novelty

This work demonstrates the significant novelty of applying an RGB colour sensor (TCS34725) for the quantitative measurement of H₂O₂ and glucose which holds great potential as a point-of-care platform for diabetic patients. Consequently, our approach broadens the use of MOFs in biosensing and presents a viable substitute for affordable, and easily accessible diabetes monitoring. These 2D bimetallic MOFs are promising materials for glucose detection applications, expanding the utility of MOFs to include biosensor applications.

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引用次数: 0

Integrating NMR and multi-LC-MS-based untargeted metabolomics for comprehensive analysis of blood serum samples

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-04 DOI: 10.1016/j.aca.2025.343979

Tereza Kacerova, Elisabete Pires, John Walsby-Tickle, Fay Probert, James S.O. McCullagh

Background

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have emerged as pivotal tools in biofluid metabolomics, facilitating investigation of disease mechanisms and biomarker discovery. Despite complementary capabilities, these techniques are rarely combined, although their integration is often beneficial. Typically, different sample preparation approaches are used, and compatibility challenges potentially arise due to the requirement for deuterated buffered solvents in NMR but not MS techniques. Additionally, MS-based approaches necessitate protein removal from samples whilst in NMR proteins can be potentially useful biomarkers. In this study, we developed a blood serum preparation protocol enabling sequential NMR and multi-LC-MS untargeted metabolomics analysis using a single serum aliquot in a research discovery setting.

Results

We analysed human serum samples using various untargeted NMR and multi-LC-MS platforms to assess the impact of deuterated solvents and buffers on detected compound-features. Employing multiple LC-MS profiling approaches, we observed no evidence of deuterium incorporation into metabolites following sample preparation with deuterated solvents. Furthermore, we demonstrated that buffers used in NMR were well tolerated by LC-MS. Protein removal, involving both solvent precipitation and molecular weight cut-off (MWCO) filtration, was identified as a primary factor influencing metabolite abundance. Our findings led to the development and validation of a serum sample preparation protocol enabling a combined NMR and multi-LC-MS analysis.

Significance

Using a single clinical serum aliquot for simultaneous untargeted profiling via NMR and multi-LC-MS represents a highly efficient alternative to current methods. This approach reduces sample volume requirements and substantially expands the potential for broader metabolome coverage. Our study offers comprehensive insights into the impact of sample preparation on complex metabolic biofluid profiles, highlighting the compatibility and complementarity of LC-MS and NMR in metabolomics research.

背景质谱（MS）和核磁共振（NMR）已成为生物流体代谢组学的重要工具，有助于研究疾病机制和发现生物标记物。尽管这些技术具有互补性，但很少将它们结合在一起，尽管它们的整合往往是有益的。通常情况下，会使用不同的样品制备方法，由于 NMR 技术需要氚代缓冲溶液，而 MS 技术不需要，因此可能会出现兼容性挑战。此外，基于 MS 的方法必须去除样本中的蛋白质，而在 NMR 中蛋白质可能是有用的生物标记。在这项研究中，我们开发了一种血清制备方案，可在研究发现环境中使用单份血清等分进行连续 NMR 和多LC-MS 非靶向代谢组学分析。结果我们使用各种非靶向 NMR 和多LC-MS 平台分析了人类血清样本，以评估氚代溶剂和缓冲液对检测到的化合物特征的影响。通过采用多种 LC-MS 分析方法，我们没有观察到使用氚代溶剂制备样品后代谢物中掺入氘的迹象。此外，我们还证明 NMR 中使用的缓冲液对 LC-MS 有很好的耐受性。蛋白质去除（包括溶剂沉淀和分子量截断（MWCO）过滤）被认为是影响代谢物丰度的主要因素。我们的研究结果促进了血清样品制备方案的开发和验证，从而实现了 NMR 和多LC-MS 联合分析。这种方法减少了对样本量的要求，并大大扩展了更广泛的代谢组覆盖范围。我们的研究全面揭示了样品制备对复杂代谢生物流体图谱的影响，凸显了 LC-MS 和 NMR 在代谢组学研究中的兼容性和互补性。

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引用次数: 0

Capillary flow of hybrid mode for enhancement of flow rate on μTADs 混合模式毛细管流提高 μTAD 上的流速

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-03 DOI: 10.1016/j.aca.2025.343996

Yixi Shi , Haonan Li , Hao Chen , Xionghui Li , Muyang Zhang , Qinghao He , Jie Zhou , Jiahua Zhong , Xinyi Chen , Huiru Zhang , Lok Ting Chu , Weijin Guo

Background

Microfluidic paper-based analytical devices (μPADs) and microfluidic paper-based analytical devices (μTADs) have already found many successful applications in biosensing. These devices mainly utilize the autonomous capillary pumping of liquid for sample actuation. Capillary flow control is very interesting and necessary for μPADs and μTADs with point-of-care diagnostic applications. Many attempts have been made on μPADs while few attempts exist for μTADs. Methods for facile flow rate control on μTADs are needed for improving the performance of μTADs on point-of-care diagnostics.

Results

Here, we develop a method for increasing the capillary flow rate on μTADs. By incorporating hollow channels within μTADs that are encapsulated by thermal contraction tubes, we establish a hybrid capillary flow mode, which significantly enhances the flow rates in these devices. We investigate the influence of hollow channel size on the capillary flow rate, and find that the average flow rate increases with the hollow channel size. The average flow rate of the group with hollow channel size as 1200 μm is 12353% more than that of the control group. Then we choose the group with hollow channel size as 1200 μm for investigation of flow rate enhancement on high viscosity liquid samples (glycerol) and non-Newtonian liquid samples (whole blood). Moreover, we also show that programmed enhancement of capillary flow rate is possible by creating μTADs with a partial hollow channel along the threads.

Significance

The simplicity of our method enables it to be used for facile flow rate control on μTADs, and it works well for various and complex liquid samples. We believe that by combination with immunoassays, it has the potential in improving the performance of μTADs for point-of-care diagnostics in the near future.

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引用次数: 0

Boosted Synergistic Catalytic Performance of Pt Single-Atom Catalyst NiS2/Pt on Wearable Hydrogel Biosensor for Sweat Lactic Acid Analysis 铂单原子催化剂 NiS2/Pt 在用于汗液乳酸分析的可穿戴水凝胶生物传感器上的增效催化性能

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-03 DOI: 10.1016/j.aca.2025.343971

Cuncun Wang , Yong Zhang , Xin Zeng , Changpeng Jin , Yiyi Liu , Mei Yang , Danqun Huo , Changjun Hou

Background

Lactic acid is an important biochemical marker for monitoring tissue oxidation, as its buildup can lead to soreness, fatigue, and potentially serious. Sweat lactic acid detection typically relies on lactic acid oxidase, but the limitations of natural enzymes restrict their use.

Results

A Pt single-atom catalyst (NiS₂/Pt) supported on NiS₂ was prepared via hydrothermal and photochemical fixation methods, and then modified onto a PI flexible electrode. This was integrated with a hydrogel GO/EG-SPB, which exhibits excellent mechanical properties and a PDMS microfluidic channel modified with 2% Silwet-L77. A sensitive, flexible electrochemical sensing platform was constructed for detecting sweat lactic acid. Through density functional theory (DFT) calculations, the optimal adsorption configurations and lowest adsorption energies of LA on the NiS₂/Pt surface were obtained, revealing the catalytic mechanism. The synergistic catalytic effect between Pt single atoms and surrounding Ni atoms significantly improves the sensor’s performance. Based on these unique advantages, the flexible sensing platform shows excellent sensing performance for sweat LA detection, including high selectivity, low detection limits (7.02 μM) and a wide linear range (10 μM - 10 mM).

Significance

The NiS₂/Pt prepared in this study has been successfully applied to accurate and sensitive lactate detection in sweat, which has promising applications in health monitoring and wearable technologies.

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引用次数: 0

Dual-channel, real-time, long-term stable electrochemical immunosensor based on Au, Cu-vertical graphene for detection of carcinoembryonic antigen in tumor cells 基于金、铜-垂直石墨烯的双通道、实时、长期稳定的电化学免疫传感器，用于检测肿瘤细胞中的癌胚抗原

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-03 DOI: 10.1016/j.aca.2025.344017

Tingting Yao , Wei Li , Hongji Li , Xiuwei Xuan , Cuiping Li , Mingji Li

Background

The accurate and rapid determination of a broad-spectrum tumor marker, carcinoembryonic antigen (CEA), in tumor cells, human tissues, and body fluids is important for the early diagnosis, drug development, efficacy evaluation, and prognosis tracking of cancer.

Results

In this study, a dual-channel electrochemical immunosensor was designed for the sensitive determination of CEA levels using an Au–AuCu-vertical graphene (VG) sensing platform. An AuCu bimetallic doping strategy was adopted to improve the biocompatibility of graphene with the cells, and Au nanoparticles were electrodeposited to firmly bind numerous CEA antibodies. The immunosensor exhibited a broad limit of linearity from 0.001 to 30000 pg mL⁻¹ and a low limit of detection of 0.28 fg mL⁻¹. This immunosensor exhibited excellent selectivity, reproducibility, and long-term stability. The developed Au–AuCu-VG-based immunosensor pen combined with self-designed electrochemical immunoassay software achieved high-precision real-time on-site analysis of CEA concentrations.

Significance

The proposed AuCu-VG electrode exhibited antibody-binding ability, inherent probe peak, and excellent binding of the Au NPs. A new dual-channel electrochemical immunoassay strategy was developed based on the AuCu-VG electrode, which could sensitively and reliably detect the real-time concentration of CEA released by tumor cells, such as MCF-7.

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引用次数: 0

The power of trapped ion mobility for isotope tracer experiments 诱捕离子迁移率在同位素示踪实验中的威力

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-02 DOI: 10.1016/j.aca.2025.344005

Karin Preindl , Chuqiao Chen , Supriya Murthy , Florian Gruber , Christian Freystätter , Thomas Weichhart , Thomas Stimpfl , Birgit Reiter , Arvand Haschemi , Gunda Koellensperger

Background

Isotope tracing experiments in cellular metabolomics are challenged by the multiple isomers and in-source fragments, which need to be considered to obtain unbiased isotopologue ratio measurements. Thus, both, selectivity and sensitivity are key requirements for customized workflows. Trapped ion mobility spectrometry (TIMS) introduces an additional separation dimension to mass spectrometry, separating otherwise co-eluting isomers by measuring the ion mobility of a molecule. This study shows for the first time, the potential of this MS platform for accurate isotopologue assessment as showcased in isotope tracer experiments using mammalian cells.

Results

The validation exercise focused on spectral accuracy, precision, and metabolite detection capabilities and comprised independent measurements on an orbitrap-based platform. Hydrophilic interaction chromatography, in combination with TIMS-TOF-MS delivered excellent results, with a minimum trueness bias and excellent precision (CV%) between 0.3 % and 6.4 %. The ion mobility separation allowed for differentiation of the otherwise co-eluting isomers fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P). Overall, isotopologue distributions were in good agreement upon crossvalidation with the orbitrap platform.

Finally, a proof-of-concept tracer study addressed the activity of the glycolysis and the pentose phosphate pathway (PPP) in resting and endotoxin activated macrophages. We confirmed an activation of glycolysis and PPP in LPS activated macrophages, but found a potentially reduced relative contribution of glucose-6-phosphate (G6P) to increased F6P pools. Our findings imply that TIMS is a powerful technology for the reliable measurements of isotope distribution analysis in metabolic tracing experiments. Significance: By implementation of ion mobility, it is now possible to generate distinct isotopologue patterns for G1P and F6P in isotope tracer experiments. F6P plays a crucial role in glycolysis and PPP, highlighting the importance of precise analytical measurements. This is particularly true for metabolic studies in immunology and cancer research.

{"title":"The power of trapped ion mobility for isotope tracer experiments","authors":"Karin Preindl , Chuqiao Chen , Supriya Murthy , Florian Gruber , Christian Freystätter , Thomas Weichhart , Thomas Stimpfl , Birgit Reiter , Arvand Haschemi , Gunda Koellensperger","doi":"10.1016/j.aca.2025.344005","DOIUrl":"10.1016/j.aca.2025.344005","url":null,"abstract":"<div><h3>Background</h3><div>Isotope tracing experiments in cellular metabolomics are challenged by the multiple isomers and in-source fragments, which need to be considered to obtain unbiased isotopologue ratio measurements. Thus, both, selectivity and sensitivity are key requirements for customized workflows. Trapped ion mobility spectrometry (TIMS) introduces an additional separation dimension to mass spectrometry, separating otherwise co-eluting isomers by measuring the ion mobility of a molecule<strong>.</strong> This study shows for the first time, the potential of this MS platform for accurate isotopologue assessment as showcased in isotope tracer experiments using mammalian cells.</div></div><div><h3>Results</h3><div>The validation exercise focused on spectral accuracy, precision, and metabolite detection capabilities and comprised independent measurements on an orbitrap-based platform. Hydrophilic interaction chromatography, in combination with TIMS-TOF-MS delivered excellent results, with a minimum trueness bias and excellent precision (CV%) between 0.3 % and 6.4 %. The ion mobility separation allowed for differentiation of the otherwise co-eluting isomers fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P). Overall, isotopologue distributions were in good agreement upon crossvalidation with the orbitrap platform.</div><div>Finally, a proof-of-concept tracer study addressed the activity of the glycolysis and the pentose phosphate pathway (PPP) in resting and endotoxin activated macrophages. We confirmed an activation of glycolysis and PPP in LPS activated macrophages, but found a potentially reduced relative contribution of glucose-6-phosphate (G6P) to increased F6P pools. Our findings imply that TIMS is a powerful technology for the reliable measurements of isotope distribution analysis in metabolic tracing experiments. <strong>Significance</strong>: By implementation of ion mobility, it is now possible to generate distinct isotopologue patterns for G1P and F6P in isotope tracer experiments. F6P plays a crucial role in glycolysis and PPP, highlighting the importance of precise analytical measurements. This is particularly true for metabolic studies in immunology and cancer research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344005"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid identification of multiplexed pathogens via a two-step dual-channel fluorescence turn-on array

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-02 DOI: 10.1016/j.aca.2025.344012

Xingliang Huang , Yang Yu , Huihai Li , Zhongzheng Xiong , Jinwu Wei , Dengqiong Yu , Hengfei Zhao , Liya Liao , Yong Li , Chao Guo , Chao Xu , Weiwei Ni , Jinsong Han , Xuejuan Gao , Hui Huang

Bacterial infections have been an increasingly serious threat to human health. However, the rapid identification of multiplexed bacteria remains challenging due to their intricate composition. Herein, we developed a two-step, dual-channel fluorescence “turn-on” sensor array that sequentially amplifies signals via Indicator Displacement Analysis (IDA) and Aggregation-Induced Emission (AIE). Three weakly fluorescent, positively charged conjugated fluorophores (A1–A3) with AIE properties were designed to form electrostatic complexes (C1–C3) with negatively charged graphene oxide (GO). Upon addition of bacteria, fluorophores were released from the electrostatic complexes via IDA, resulting in fluorescence turn-on. These fluorophores then aggregated on the bacterial surface, further enhancing fluorescence. This array accurately differentiated among 10 distinct bacterial strains, achieving 98.3 % classification accuracy within 30 s. Finally, the approach facilitated semi-quantitative bacterial analysis, multiplex identification, and robust differentiation in artificial urine samples, presenting a promising method for early infectious disease diagnosis.

{"title":"Rapid identification of multiplexed pathogens via a two-step dual-channel fluorescence turn-on array","authors":"Xingliang Huang , Yang Yu , Huihai Li , Zhongzheng Xiong , Jinwu Wei , Dengqiong Yu , Hengfei Zhao , Liya Liao , Yong Li , Chao Guo , Chao Xu , Weiwei Ni , Jinsong Han , Xuejuan Gao , Hui Huang","doi":"10.1016/j.aca.2025.344012","DOIUrl":"10.1016/j.aca.2025.344012","url":null,"abstract":"<div><div>Bacterial infections have been an increasingly serious threat to human health. However, the rapid identification of multiplexed bacteria remains challenging due to their intricate composition. Herein, we developed a two-step, dual-channel fluorescence “turn-on” sensor array that sequentially amplifies signals <em>via</em> Indicator Displacement Analysis (IDA) and Aggregation-Induced Emission (AIE). Three weakly fluorescent, positively charged conjugated fluorophores (<strong>A1</strong>–<strong>A3</strong>) with AIE properties were designed to form electrostatic complexes (<strong>C1</strong>–<strong>C3</strong>) with negatively charged graphene oxide (<strong>GO</strong>). Upon addition of bacteria, fluorophores were released from the electrostatic complexes <em>via</em> IDA, resulting in fluorescence turn-on. These fluorophores then aggregated on the bacterial surface, further enhancing fluorescence. This array accurately differentiated among 10 distinct bacterial strains, achieving 98.3 % classification accuracy within 30 s. Finally, the approach facilitated semi-quantitative bacterial analysis, multiplex identification, and robust differentiation in artificial urine samples, presenting a promising method for early infectious disease diagnosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344012"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic characterisation of microplastics released from disposable medical devices using laser direct infrared spectroscopy

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-02 DOI: 10.1016/j.aca.2025.343982

Chuanfeng Chen, Shanshan Du, Ziyan Liu, Wenfei Li, Fangbiao Tao, Xuejiao Qie

Background

Human exposure to microplastics (MPs) is widespread, attracting significant attention from both the public and the scientific community. Although several direct and indirect exposure pathways have been investigated, the extent of MP exposure from disposable medical devices remains poorly understood and warrants further research.

Results

This work indicates that many MPs (10-30 μm) were released during the simulated use of disposable medical devices. Two common medical devices–disposable infusion tubes and blood needles–were selected as the research subjects. Analysis utilizing laser direct infrared (LDIR) revealed that plastic released from infusion tubes primarily consisted of polyamide (PA), polyvinyl chloride (PVC), and polyethene terephthalate (PET), with an average total number (ATN) of 11.8 particles/mL. MPs released from blood collection needles mainly consisted of polyurethane (PU) and PET, with an ATN of 82.7 particles/mL. For a 0.9% normal saline, the ATN released from the infusion tubes during the stimulating infusion scenario at room temperature (4 h) was approximately 16 particles/mL, primarily consisting of PA, PVC, and PET. Additionally, the release of MPs increased with rising temperatures. Under the same conditions, ATN release from the blood collection needles was approximately 84.4 particles/mL, mainly from PA, PVC, and PU.

Significance

This implies that MPs can enter the bloodstream directly through infusion tubes and blood collection needles, highlighting the need for greater attention to the risk of patient exposure.

{"title":"Systematic characterisation of microplastics released from disposable medical devices using laser direct infrared spectroscopy","authors":"Chuanfeng Chen, Shanshan Du, Ziyan Liu, Wenfei Li, Fangbiao Tao, Xuejiao Qie","doi":"10.1016/j.aca.2025.343982","DOIUrl":"10.1016/j.aca.2025.343982","url":null,"abstract":"<div><h3>Background</h3><div>Human exposure to microplastics (MPs) is widespread, attracting significant attention from both the public and the scientific community. Although several direct and indirect exposure pathways have been investigated, the extent of MP exposure from disposable medical devices remains poorly understood and warrants further research.</div></div><div><h3>Results</h3><div>This work indicates that many MPs (10-30 μm) were released during the simulated use of disposable medical devices. Two common medical devices–disposable infusion tubes and blood needles–were selected as the research subjects. Analysis utilizing laser direct infrared (LDIR) revealed that plastic released from infusion tubes primarily consisted of polyamide (PA), polyvinyl chloride (PVC), and polyethene terephthalate (PET), with an average total number (ATN) of 11.8 particles/mL. MPs released from blood collection needles mainly consisted of polyurethane (PU) and PET, with an ATN of 82.7 particles/mL. For a 0.9% normal saline, the ATN released from the infusion tubes during the stimulating infusion scenario at room temperature (4 h) was approximately 16 particles/mL, primarily consisting of PA, PVC, and PET. Additionally, the release of MPs increased with rising temperatures. Under the same conditions, ATN release from the blood collection needles was approximately 84.4 particles/mL, mainly from PA, PVC, and PU.</div></div><div><h3>Significance</h3><div>This implies that MPs can enter the bloodstream directly through infusion tubes and blood collection needles, highlighting the need for greater attention to the risk of patient exposure.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 343982"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen peroxide regulated split-type electrochemiluminescence sensing platform for non-invasive detection of gastric cancer-associated D-amino acids

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-02 DOI: 10.1016/j.aca.2025.344010

Juan Li , Ming-Chun Lai , Ya-Meng Zhong , Ya-Ling Chen , Na Wu , Wei Chen , Hua-Ping Peng

Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective d-alanine (D-Ala) ECL assay strategy via a turn-off-on split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H₂O₂) could be used as an efficient etching agent to turn on the MnO₂/gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H₂O₂. Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0 × 10 ⁻¹⁰∼1.0 × 10⁻³ mol L⁻¹, and realized the detection of D-Ala as low as 2.2 × 10⁻¹¹ mol L⁻¹ (S/N = 3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H₂O₂ to MnO₂ nanomaterials on electrode surface. In our perception, this H₂O₂ mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.

{"title":"Hydrogen peroxide regulated split-type electrochemiluminescence sensing platform for non-invasive detection of gastric cancer-associated D-amino acids","authors":"Juan Li , Ming-Chun Lai , Ya-Meng Zhong , Ya-Ling Chen , Na Wu , Wei Chen , Hua-Ping Peng","doi":"10.1016/j.aca.2025.344010","DOIUrl":"10.1016/j.aca.2025.344010","url":null,"abstract":"<div><div>Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective <span>d</span>-alanine (D-Ala) ECL assay strategy via a turn-off-on split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) could be used as an efficient etching agent to turn on the MnO<sub>2</sub>/gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H<sub>2</sub>O<sub>2</sub>. Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0 × 10 <strong><sup>−</sup></strong><sup>10</sup>∼1.0 × 10<strong><sup>−</sup></strong><sup>3</sup> mol L<strong><sup>−1</sup></strong>, and realized the detection of D-Ala as low as 2.2 × 10<strong><sup>−</sup></strong><sup>11</sup> mol L<strong><sup>−1</sup></strong> (S/N = 3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H<sub>2</sub>O<sub>2</sub> to MnO<sub>2</sub> nanomaterials on electrode surface. In our perception, this H<sub>2</sub>O<sub>2</sub> mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1355 ","pages":"Article 344010"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rotary fluorescence array microfluidic chip for rapid detection of multiple pathogens in foods using dual-discs assisted recycling for signal amplification

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta

Pub Date : 2025-04-02 DOI: 10.1016/j.aca.2025.344014

Bailu Liu , Zhenli Xu , Zhijian Jia , Huihui Wu , Tianhua Li , Zhenzhong Yu , Ning Gan

Background

The ability to rapidly and on-site detect multiple pathogens in food is crucial for food safety monitoring. The microfluidic chip integrated with aptamer (Apt) probes stands as a powerful tool for this venture. However, the utility of these chips has been constrained by the need for cumbersome fluid control devices, such as syringe pumps and bulky optical detectors, so the development of highly integrated integrated chips has become one of the priorities of biosensor development in recent years.

Result

In this work, a novel rotary fluorescence array microfluidic chip has been engineered for the simultaneous and rapid detection of three pathogens. The chip has three different detection regions and a double-layer structure composed of PDMS and glass. The Apts and its complementary DNA strands were immobilized on the gold nanoparticle layer in the detection region for pathogen identification, the fluorescence (FL) from the double-stranded staining solution added after the reaction was used for signal amplification. Different arrays of gold discs can be employed to identify various bacteria. This method enables the detection of Staphylococcus aureus (S.A), Salmonella typhimurium (S.T), and Vibrio parahemolyticus (V.P) in food samples within 30 min at a sensitivity of 7–39 CFU/mL.

Significance

The microfluidic chip has good specificity and sensitivity while avoiding cross interference, and the ratio form of quantification avoids background light interference. Different arrays can be used to identify different bacteria, which is of great significance for the rapid detection of a variety of foodborne pathogens in the field.

{"title":"Rotary fluorescence array microfluidic chip for rapid detection of multiple pathogens in foods using dual-discs assisted recycling for signal amplification","authors":"Bailu Liu , Zhenli Xu , Zhijian Jia , Huihui Wu , Tianhua Li , Zhenzhong Yu , Ning Gan","doi":"10.1016/j.aca.2025.344014","DOIUrl":"10.1016/j.aca.2025.344014","url":null,"abstract":"<div><h3>Background</h3><div>The ability to rapidly and on-site detect multiple pathogens in food is crucial for food safety monitoring. The microfluidic chip integrated with aptamer (Apt) probes stands as a powerful tool for this venture. However, the utility of these chips has been constrained by the need for cumbersome fluid control devices, such as syringe pumps and bulky optical detectors, so the development of highly integrated integrated chips has become one of the priorities of biosensor development in recent years.</div></div><div><h3>Result</h3><div>In this work, a novel rotary fluorescence array microfluidic chip has been engineered for the simultaneous and rapid detection of three pathogens. The chip has three different detection regions and a double-layer structure composed of PDMS and glass. The Apts and its complementary DNA strands were immobilized on the gold nanoparticle layer in the detection region for pathogen identification, the fluorescence (FL) from the double-stranded staining solution added after the reaction was used for signal amplification. Different arrays of gold discs can be employed to identify various bacteria. This method enables the detection of <em>Staphylococcus aureus</em> (<em>S.A</em>), <em>Salmonella typhimurium</em> (<em>S.T</em>), and <em>Vibrio parahemolyticus</em> (<em>V.P</em>) in food samples within 30 min at a sensitivity of 7–39 CFU/mL.</div></div><div><h3>Significance</h3><div>The microfluidic chip has good specificity and sensitivity while avoiding cross interference, and the ratio form of quantification avoids background light interference. Different arrays can be used to identify different bacteria, which is of great significance for the rapid detection of a variety of foodborne pathogens in the field.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1356 ","pages":"Article 344014"},"PeriodicalIF":5.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0