Pub Date : 2025-12-19DOI: 10.1016/j.aca.2025.345028
Lu Lin, Mengmeng Lv, Jiangtao Ren, Jin Wang, Erkang Wang
{"title":"Investigation of intramolecular split G-quadruplex-hemin systems and development of DNAzyme-based probes","authors":"Lu Lin, Mengmeng Lv, Jiangtao Ren, Jin Wang, Erkang Wang","doi":"10.1016/j.aca.2025.345028","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345028","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"93 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-19DOI: 10.1016/j.aca.2025.345023
Swapnita Patra, Krishna Manjari Sahu, Roshan K. Bhoi, Sarat K. Swain
{"title":"CQD Imprinted Pectin for Ex Vivo Glucose Monitoring in Human Blood Serum: Mechanistic Insights into Competitive Esterification-Driven Fluorescence Quenching","authors":"Swapnita Patra, Krishna Manjari Sahu, Roshan K. Bhoi, Sarat K. Swain","doi":"10.1016/j.aca.2025.345023","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345023","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"11 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-19DOI: 10.1016/j.aca.2025.345033
Hanieh Kefayati, Yadollah Yamini
{"title":"Innovative configuration of multiple MIL-100(Fe) monolithic columns for in-tube solid-phase microextraction of sulfonamides in human tear fluid and milk samples","authors":"Hanieh Kefayati, Yadollah Yamini","doi":"10.1016/j.aca.2025.345033","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345033","url":null,"abstract":"","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"178 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we developed an LC-MS/MS method that enables baseline separation and quantification of α- and β-nicotinamide mononucleotide (NMN) isomers under fully aqueous reversed-phase conditions. At first, three commonly used reversed-phase columns (Polar C18, F5, and PBr) were evaluated, and only the PBr phase provided sufficient stereoselectivity through enhanced π-π stacking, dipole-dipole interactions, and halogen bonding. Using 0.1% formic acid in water as the mobile phase, the two isomers were baseline resolved within three minutes. The method was validated for linearity, sensitivity, precision, accuracy, and matrix effects. When applied to eight commercial NMN supplements, the analysis revealed substantial variation in isomer composition, including undeclared or inaccurately labeled ingredients. Overall, this work presents the first LC-MS/MS approach capable of isomer-specific NMN analysis under fully aqueous reversed-phase conditions, offering a robust and environmentally compatible platform for product authentication, quality control, and potential applications in pharmacokinetics and metabolomics.
{"title":"Aqueous LC-MS/MS quantification of α-/β-nicotinamide mononucleotide in dietary supplements using a pentabromophenyl column","authors":"Chng Sze Hoei, Nian-Hua Wu, Chao-Ming Tsen, Ping-Wei Sun, Han-Wei Chang, Shin-Yuan Wang, Hung-Yu Lin","doi":"10.1016/j.aca.2025.345027","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345027","url":null,"abstract":"In this study, we developed an LC-MS/MS method that enables baseline separation and quantification of α- and β-nicotinamide mononucleotide (NMN) isomers under fully aqueous reversed-phase conditions. At first, three commonly used reversed-phase columns (Polar C18, F5, and PBr) were evaluated, and only the PBr phase provided sufficient stereoselectivity through enhanced π-π stacking, dipole-dipole interactions, and halogen bonding. Using 0.1% formic acid in water as the mobile phase, the two isomers were baseline resolved within three minutes. The method was validated for linearity, sensitivity, precision, accuracy, and matrix effects. When applied to eight commercial NMN supplements, the analysis revealed substantial variation in isomer composition, including undeclared or inaccurately labeled ingredients. Overall, this work presents the first LC-MS/MS approach capable of isomer-specific NMN analysis under fully aqueous reversed-phase conditions, offering a robust and environmentally compatible platform for product authentication, quality control, and potential applications in pharmacokinetics and metabolomics.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"58 1","pages":"345027"},"PeriodicalIF":6.2,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-17DOI: 10.1016/j.aca.2025.345016
César Castro-García, Arianna Palermo, Verónica Rodríguez-Saldaña, Edwin Palacio, Luz O. Leal-Quezada, Laura Ferrer
This study presents a novel approach for the simultaneous microextraction of six pharmaceutical products based on the use of three different deep eutectic solvents (DES) as supported liquid membranes (SLM), exploiting a custom-designed 3D-printed multi-compartment device for the electromembrane extraction (EME) and subsequent ultra-high-performance liquid chromatography–diode array detector (UHPLC-DAD) analysis. Three DES were synthesized and characterized, combining menthol with aliphatic acids in a 2:1 molar ratio. Thus, menthol:heptanoic acid was used for the extraction of tetracycline (TET) and ampicillin (AMP), menthol:octanoic acid for ciprofloxacin (CIP) and sulfamethoxazole (SMX), while menthol:decanoic acid was used for the extraction of diclofenac (DIC) and ibuprofen (IBU). The simultaneous extraction was optimized by evaluating key parameters such as extraction time, applied voltage, agitation speed, DES volume, buffer composition, and pH in both the donor and acceptor phases. Under optimal conditions, the method achieved limits of detection ranging from 0.05 to 0.4 μg L-1, with enrichment factors between 10.7 and 13.1. The proposed methodology was satisfactorily applied to wastewater samples, obtaining high relative recoveries (83–102%) and good reproducibility (precision < 9% RSD). The study demonstrates that the combination of 3D-printed devices, DES-SLM, and EME constitutes a robust, reproducible, and environmentally friendly analytical strategy for monitoring pharmaceutical pollutants in water matrices.
{"title":"Electromembrane Extraction Using Deep Eutectic Solvent-Supported Liquid Membranes in a 3D-Printed Multi-Compartment Device for Multi-Class Pharmaceutical Analysis in Wastewater","authors":"César Castro-García, Arianna Palermo, Verónica Rodríguez-Saldaña, Edwin Palacio, Luz O. Leal-Quezada, Laura Ferrer","doi":"10.1016/j.aca.2025.345016","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345016","url":null,"abstract":"This study presents a novel approach for the simultaneous microextraction of six pharmaceutical products based on the use of three different deep eutectic solvents (DES) as supported liquid membranes (SLM), exploiting a custom-designed 3D-printed multi-compartment device for the electromembrane extraction (EME) and subsequent ultra-high-performance liquid chromatography–diode array detector (UHPLC-DAD) analysis. Three DES were synthesized and characterized, combining menthol with aliphatic acids in a 2:1 molar ratio. Thus, menthol:heptanoic acid was used for the extraction of tetracycline (TET) and ampicillin (AMP), menthol:octanoic acid for ciprofloxacin (CIP) and sulfamethoxazole (SMX), while menthol:decanoic acid was used for the extraction of diclofenac (DIC) and ibuprofen (IBU). The simultaneous extraction was optimized by evaluating key parameters such as extraction time, applied voltage, agitation speed, DES volume, buffer composition, and pH in both the donor and acceptor phases. Under optimal conditions, the method achieved limits of detection ranging from 0.05 to 0.4 μg L<sup>-1</sup>, with enrichment factors between 10.7 and 13.1. The proposed methodology was satisfactorily applied to wastewater samples, obtaining high relative recoveries (83–102%) and good reproducibility (precision < 9% RSD). The study demonstrates that the combination of 3D-printed devices, DES-SLM, and EME constitutes a robust, reproducible, and environmentally friendly analytical strategy for monitoring pharmaceutical pollutants in water matrices.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1 1","pages":"345016"},"PeriodicalIF":6.2,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salicylic acid (SA) is a plant hormone playing a key role in plant innate immunity and stress adaptation. The sensitivity of reported SA immunoassays cannot meet the requirements of practical sample detection.
Results
In the present work, SA was coupled with bovine serum albumin as an immunogen via bis-(diazo)benzidine. A monoclonal antibody (designated as mAb 4G3) specifically recognizing the p-diazobenzoic acid (p-DBA) derivatized SA was successfully generated. The sensitivity was more than 1000-fold greater than that reported free SA immunoassays. After systematic optimization of SA derivation procedure, the half maximum inhibition concentration and detection range of the indirect competitive enzyme linked immunosorbent assay (icELISA) were 18 ng/mL and 5.1 – 71 ng/mL of SA, respectively. The icELISA showed no cross-reactivity to other SA analogs and only 8% cross-reactivity to p-DBA derivatized aspirin. The average recoveries of SA fortified in tobacco leaves ranged from 81% to 104% and were verified with high-performance liquid chromatography-mass spectrometry. The icELISA could accurately determine SA content in tobacco leaves in response to tobacco mosaic virus inoculation and drought stress.
Significance
The SA immunoassay offers an accurate and cost-effective method for routine detection of SA. This work provides a new strategy for developing a highly sensitive and specific immunoassay for structurally simple and small molecules, particularly those containing carboxyl groups.
{"title":"Development and application of a sensitive and specific monoclonal antibody-based ELISA for the analysis of salicylic acid in tobacco plants","authors":"Yixuan Wu, Yujie Chen, Haiyan Xing, Jiaqi Zhang, Yang Shi, Yuting He, Yuqing Xue, Mingcai Zhang, Baomin Wang, Qing X. Li","doi":"10.1016/j.aca.2025.345019","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345019","url":null,"abstract":"<h3>Background</h3>Salicylic acid (SA) is a plant hormone playing a key role in plant innate immunity and stress adaptation. The sensitivity of reported SA immunoassays cannot meet the requirements of practical sample detection.<h3>Results</h3>In the present work, SA was coupled with bovine serum albumin as an immunogen via <em>bis</em>-(diazo)benzidine. A monoclonal antibody (designated as mAb 4G3) specifically recognizing the <em>p</em>-diazobenzoic acid (<em>p</em>-DBA) derivatized SA was successfully generated. The sensitivity was more than 1000-fold greater than that reported free SA immunoassays. After systematic optimization of SA derivation procedure, the half maximum inhibition concentration and detection range of the indirect competitive enzyme linked immunosorbent assay (icELISA) were 18 ng/mL and 5.1 – 71 ng/mL of SA, respectively. The icELISA showed no cross-reactivity to other SA analogs and only 8% cross-reactivity to <em>p</em>-DBA derivatized aspirin. The average recoveries of SA fortified in tobacco leaves ranged from 81% to 104% and were verified with high-performance liquid chromatography-mass spectrometry. The icELISA could accurately determine SA content in tobacco leaves in response to tobacco mosaic virus inoculation and drought stress.<h3>Significance</h3>The SA immunoassay offers an accurate and cost-effective method for routine detection of SA. This work provides a new strategy for developing a highly sensitive and specific immunoassay for structurally simple and small molecules, particularly those containing carboxyl groups.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"156 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-15DOI: 10.1016/j.aca.2025.345017
Benedetta Niccolini, Riccardo Di Santo, Flavio Di Giacinto, Linda Tartaglione, Alessandro Rizzi, Maria Laura Leo, Letizia Masi, Valentina Petito, Franco Scaldaferri, Marco De Spirito, Dario Pitocco, Gabriele Ciasca
Diabetic sensorimotor peripheral neuropathy (DSPN) is the most prevalent neuropathic complication of diabetes mellitus (DM), significantly impacting the patient's quality of life and correlating with increased mortality. Early intervention is key to slowing down the progression of DSPN, underscoring the importance of a timely diagnosis, currently based on patients' medical history and physical examinations. In this study, we aim to identify novel markers of DSPN in type 1 DM (T1DM) patients by investigating several blood components, including plasma, red blood cells (RBCs), RBC-ghosts (RBC-G), and plasma-extracellular vesicles (pEVs).
{"title":"Spectral Analysis of Blood Components in Diabetic Patients with Neuropathic Complications: Exploring the Diagnostic Potential of Extracellular Vesicles","authors":"Benedetta Niccolini, Riccardo Di Santo, Flavio Di Giacinto, Linda Tartaglione, Alessandro Rizzi, Maria Laura Leo, Letizia Masi, Valentina Petito, Franco Scaldaferri, Marco De Spirito, Dario Pitocco, Gabriele Ciasca","doi":"10.1016/j.aca.2025.345017","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345017","url":null,"abstract":"Diabetic sensorimotor peripheral neuropathy (DSPN) is the most prevalent neuropathic complication of diabetes mellitus (DM), significantly impacting the patient's quality of life and correlating with increased mortality. Early intervention is key to slowing down the progression of DSPN, underscoring the importance of a timely diagnosis, currently based on patients' medical history and physical examinations. In this study, we aim to identify novel markers of DSPN in type 1 DM (T1DM) patients by investigating several blood components, including plasma, red blood cells (RBCs), RBC-ghosts (RBC-G), and plasma-extracellular vesicles (pEVs).","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"20 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid, low-cost measurement of glutathione (GSH) is valuable for judging food freshness. However, many colorimetric assays depend on natural enzymes or added H2O2, which limits stability and use in real samples.
Results
We developed a manganese single-atom enzyme (Mn/SAE) with intrinsic oxidase-like activity to build an enzyme-free, H2O2-free colorimetric system for GSH. Mn/SAE uses dissolved O2 to oxidize TMB to blue oxTMB; GSH reduces oxTMB back to TMB, producing a decrease in color and absorbance for readout. Under optimized conditions, both the solution assay and a paper-based analytical device (PAD) provide results within 10 min using smartphone R-channel quantification. The method shows good selectivity and performs well in real samples, tracking the expected decline of GSH in strawberries during storage.
Significance
This simple, low-cost, and portable approach enables on-site GSH analysis without enzymes or H2O2. The Mn/SAE colorimetric system offers practical value for freshness monitoring and can be adapted to other redox biomarkers in food-quality assessment.
{"title":"Dissolved-O2–Driven Mn Single-Atom Enzyme for colorimetric detection of Glutathione in food","authors":"Lin Wang, Yue Wang, Xiao-Jie Shi, Tian-Shun Song, Xiang-Ling Li, Jing-Jing Xie","doi":"10.1016/j.aca.2025.345018","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345018","url":null,"abstract":"<h3>Background</h3>Rapid, low-cost measurement of glutathione (GSH) is valuable for judging food freshness. However, many colorimetric assays depend on natural enzymes or added H<sub>2</sub>O<sub>2</sub>, which limits stability and use in real samples.<h3>Results</h3>We developed a manganese single-atom enzyme (Mn/SAE) with intrinsic oxidase-like activity to build an enzyme-free, H<sub>2</sub>O<sub>2</sub>-free colorimetric system for GSH. Mn/SAE uses dissolved O<sub>2</sub> to oxidize TMB to blue oxTMB; GSH reduces oxTMB back to TMB, producing a decrease in color and absorbance for readout. Under optimized conditions, both the solution assay and a paper-based analytical device (PAD) provide results within 10 min using smartphone R-channel quantification. The method shows good selectivity and performs well in real samples, tracking the expected decline of GSH in strawberries during storage.<h3>Significance</h3>This simple, low-cost, and portable approach enables on-site GSH analysis without enzymes or H<sub>2</sub>O<sub>2</sub>. The Mn/SAE colorimetric system offers practical value for freshness monitoring and can be adapted to other redox biomarkers in food-quality assessment.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"21 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-15DOI: 10.1016/j.aca.2025.345012
Ye Chen, Zihui Yang, Xiyue Xiong, Feng chang, Yingzhuang Chen, Ming Ma, Bo Chen
The separation of highly polar and ionic compounds remains a significant challenge in hydrophilic interaction chromatography (HILIC). While zwitterionic monolithic columns are promising, their preparation often involves complex, multi-step synthesis procedures or relies on monomers that are difficult to obtain, limiting their wider application and reproducibility. This study aimed to develop a novel, simplified, and highly efficient method for synthesizing a sulfoalkyl betaine-type zwitterionic monolithic column for high-performance separation of diverse polar analytes.
{"title":"Preparation of Ultra-High Efficiency Sulfoalkyl Betaine-Type Monolithic Columns through Amine-Epoxide Ring-Opening Reaction Combined with In-Situ Ring-Opening Quaternization and Their Application in Hydrophilic Interaction Chromatography","authors":"Ye Chen, Zihui Yang, Xiyue Xiong, Feng chang, Yingzhuang Chen, Ming Ma, Bo Chen","doi":"10.1016/j.aca.2025.345012","DOIUrl":"https://doi.org/10.1016/j.aca.2025.345012","url":null,"abstract":"The separation of highly polar and ionic compounds remains a significant challenge in hydrophilic interaction chromatography (HILIC). While zwitterionic monolithic columns are promising, their preparation often involves complex, multi-step synthesis procedures or relies on monomers that are difficult to obtain, limiting their wider application and reproducibility. This study aimed to develop a novel, simplified, and highly efficient method for synthesizing a sulfoalkyl betaine-type zwitterionic monolithic column for high-performance separation of diverse polar analytes.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"152 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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