Intensive Care Unit (ICU) a part of the hospital with specialized staff and equipment specially to the observation, care and treatment of patients suffering from life threatening complications. The importance of knowing the mapping of bacterial is to know the types of bacterial and the resistance of bacterial to the therapy that will be given. The research used is descriptive by taking primary data. Criteria of research sample in the form of blood culture in patients in ICU RSD. Dr. Soebandi Jember. Sampling method is by using total sampling technique. The results is S. xylosus (55.60%), S. epidermidis (11.10%), Enterobacter cloacea (11.10%) and Stenotrophomonas maltophilia (11.105). Sensitivity test results of Staphylococcus xylosus bacteria showed resistant to class III cephalosporin group, Erythromycin, penicillin, clindamycin and macroloid. While the sensitive to chloramphenicol, linezolid, glikopeptida and vancomycin. The results of the antibiotic sensitivity test of Staphylococcus epidermidis, Enterobacter cloacea, and Stenotrophomonas maltophilia are sensitive to tetracycline and aminoglycoside antibiotics. Meanwhile, antibiotic sensitivity test of Staphylococcus epidermidis and Streptococcus sp are resistant to antibiotic penicillins, fourth generation cephalosporin, erythromycin, azithromycin. Streptococcus sp and Stenotrophomonas maltophilia are resistant to ceftriaxone antibiotic.
{"title":"Bacterial Mapping Toward Patients in Intensive Care Unit Dr. Soebandi Jember Hospital","authors":"A. Fauzi, M. A. Shodikin, Yuli Hermansyah","doi":"10.19184/ams.v5i3.6821","DOIUrl":"https://doi.org/10.19184/ams.v5i3.6821","url":null,"abstract":"Intensive Care Unit (ICU) a part of the hospital with specialized staff and equipment specially to the observation, care and treatment of patients suffering from life threatening complications. The importance of knowing the mapping of bacterial is to know the types of bacterial and the resistance of bacterial to the therapy that will be given. The research used is descriptive by taking primary data. Criteria of research sample in the form of blood culture in patients in ICU RSD. Dr. Soebandi Jember. Sampling method is by using total sampling technique. The results is S. xylosus (55.60%), S. epidermidis (11.10%), Enterobacter cloacea (11.10%) and Stenotrophomonas maltophilia (11.105). Sensitivity test results of Staphylococcus xylosus bacteria showed resistant to class III cephalosporin group, Erythromycin, penicillin, clindamycin and macroloid. While the sensitive to chloramphenicol, linezolid, glikopeptida and vancomycin. The results of the antibiotic sensitivity test of Staphylococcus epidermidis, Enterobacter cloacea, and Stenotrophomonas maltophilia are sensitive to tetracycline and aminoglycoside antibiotics. Meanwhile, antibiotic sensitivity test of Staphylococcus epidermidis and Streptococcus sp are resistant to antibiotic penicillins, fourth generation cephalosporin, erythromycin, azithromycin. Streptococcus sp and Stenotrophomonas maltophilia are resistant to ceftriaxone antibiotic.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"369 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123486914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish
{"title":"Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro","authors":"Sarwendah Siswi Winasis, Al Munawir, A. Handoko","doi":"10.19184/AMS.V5I2.9623","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.9623","url":null,"abstract":"There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. \u0000Keywords: Physalia utriculus, cocoa, toxin, jellyfish","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134447175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Most of the Indonesia population work as a farmer. Pesticides are used in agriculture sector for pest and plant diseases. Pesticides can cause intoxication. The World Health Organization (WHO) estimates that 1-5 million cases of pesticides poisoning among agricultural workers occur in developing countries. Indonesia pesticide poisoning cases reach 771 cases. Organophosphate enter the body through inhalation. Organophosphate pesticides work systemic to inhibit the cholinesterase enzyme causing lung function disorders. The purpose of this study was to analyze the correlation of cholinesterase levels to lung function in farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency. This is an observational analytic study using a cross sectional design. Cholinesterase levels test using the DGKC method to determine the presence of poisoning, while for lung function test using spirometry with FVC, FEV1 and FEV1 / FVC ratios as parameters. The results of the test in 30 samples showed 14.33% (4/30) abnormal cholinesterase levels or decreased. Lung function test showed 20% (6/30) had obstructive disorders, 43.33% (13/30) restrictive disorders and 36.67% (11/30) were normal. Data analysis using chi square showed a significant relationship (p = 0.049) between cholinesterase levels and lung function. Conclusion: There is a significant relationship between the level of cholinesterase to decrease in pulmonary physiology of farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency. Keyword :Organophosphate, cholinesterase level, lung function
{"title":"Correlation of Cholinesterase Levels to Lung Function in Farmer Exposed by Organophosphate Pesticides in Sukorambi Village, Jember Regency","authors":"P. Sandra, K. Sofiana, I. Sutejo","doi":"10.19184/AMS.V5I2.9651","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.9651","url":null,"abstract":"Abstract \u0000 \u0000Most of the Indonesia population work as a farmer. Pesticides are used in agriculture sector for pest and plant diseases. Pesticides can cause intoxication. The World Health Organization (WHO) estimates that 1-5 million cases of pesticides poisoning among agricultural workers occur in developing countries. Indonesia pesticide poisoning cases reach 771 cases. Organophosphate enter the body through inhalation. Organophosphate pesticides work systemic to inhibit the cholinesterase enzyme causing lung function disorders. The purpose of this study was to analyze the correlation of cholinesterase levels to lung function in farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency. This is an observational analytic study using a cross sectional design. Cholinesterase levels test using the DGKC method to determine the presence of poisoning, while for lung function test using spirometry with FVC, FEV1 and FEV1 / FVC ratios as parameters. The results of the test in 30 samples showed 14.33% (4/30) abnormal cholinesterase levels or decreased. Lung function test showed 20% (6/30) had obstructive disorders, 43.33% (13/30) restrictive disorders and 36.67% (11/30) were normal. Data analysis using chi square showed a significant relationship (p = 0.049) between cholinesterase levels and lung function. Conclusion: There is a significant relationship between the level of cholinesterase to decrease in pulmonary physiology of farmers exposed to organophosphate pesticides in Sukorambi Village, Jember Regency. \u0000 \u0000Keyword :Organophosphate, cholinesterase level, lung function \u0000 ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130390475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ekvan Danang Setya Pramudito, Erfan Efendi, M. A. Shodikin
Recently, cancer is globally the second highest cause of death with a total death about 8.8 million in 2015. There are some therapy of cancer, one of them by using cyclophosphamid chemotherapy, but this regimen has side effects of bone marrow depression through its active metabolite of 4 hydroxy cyclophpsphamid and increase ROS which will effect on serum MDA elevation. Increased MDA serum can be prevented by giving an antioxidant or plants that have various antioxidants, such as kenitu leaves. The aim of this study was to determine the effect of antioxidants in kenitu leaves (Chrysophyllum cainito L.)) extract to MDA serum leves of cyclophosphamid-induced mice. This study used quasi experimental method with post test only controlled group design by using 1 normal control group, 1 negative control group (cyclophosphamid), and 5 treatment groups (dose of extract 50mg/kgBW; 100mg/kgBW; 200mg/kgBW; 400mg/kgBB; and 800mg / kgBW). The results of the treatment group dose 100mg/kgBB showed the average of MDA serum levels was 1.94 μg/mL which is the highest decrease in this study. The result of Kendall Tau correlation test showed the effect of kenitu leaves extract to MDA serum level had p = 0.013 and correlation coefficient = 0.431. Keywords: kenitu leaf etanol extract, cyclophosphamid, MDA serum
{"title":"Antioxidant Effect of Kenitu leaf Etanol Extract (Chrysophyllum Cainito L.) on Wistar Rat Induced Cyclophosphamid","authors":"Ekvan Danang Setya Pramudito, Erfan Efendi, M. A. Shodikin","doi":"10.19184/AMS.V5I2.6837","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.6837","url":null,"abstract":"Recently, cancer is globally the second highest cause of death with a total death about 8.8 million in 2015. There are some therapy of cancer, one of them by using cyclophosphamid chemotherapy, but this regimen has side effects of bone marrow depression through its active metabolite of 4 hydroxy cyclophpsphamid and increase ROS which will effect on serum MDA elevation. Increased MDA serum can be prevented by giving an antioxidant or plants that have various antioxidants, such as kenitu leaves. The aim of this study was to determine the effect of antioxidants in kenitu leaves (Chrysophyllum cainito L.)) extract to MDA serum leves of cyclophosphamid-induced mice. This study used quasi experimental method with post test only controlled group design by using 1 normal control group, 1 negative control group (cyclophosphamid), and 5 treatment groups (dose of extract 50mg/kgBW; 100mg/kgBW; 200mg/kgBW; 400mg/kgBB; and 800mg / kgBW). The results of the treatment group dose 100mg/kgBB showed the average of MDA serum levels was 1.94 μg/mL which is the highest decrease in this study. The result of Kendall Tau correlation test showed the effect of kenitu leaves extract to MDA serum level had p = 0.013 and correlation coefficient = 0.431. \u0000 \u0000Keywords: kenitu leaf etanol extract, cyclophosphamid, MDA serum ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128440447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Culex quinquefasciatus was one of a vector of filariasis disease caused by filarial worm infection. Mode of transmission was through mosquito bites that contain filaria worm. To overcome the problem in terms of eradicating the mosquito larvae was by searching for safe biological materials for humans and the environment, one of them was Phyllanthus acidus leaves. The purposes of this research were to know whether the ethanol extract of Phyllanthus acidus leaves have larvicidal activity on Culex quinquefasciatus instar III / IV larvae and to know the lethal concentration 50 (LC50) ethanol extract of Phyllanthus acidus leaves on Culex quinquefasciatus instar III / IV larvae within 24 hours. This study used true experimental method with post test only controlled group design with 1 positive control group (Temephos), 1 negative control group (Aquades and DMSO), and 5 treatment groups (0.0625%; 0.125%; 0.25%, 0.5% and 1% extract) respectively 20 Culex quinquefasciatus instar III/IV larvae. The result of regression test of inverse to know the effect of leaf extract of ceremai to the death of Culex quinquefasciatus larvae obtained R2=0909 which was mean the effect of ethanol extract of Phyllanthus acidus leaves to the number of the death of Culex quinquefasciatus larvae equal to 90.9%. The result of probit test obtained LC50 result of 0.189% with 95% confidence interval (0.142% -0.233%).
{"title":"The Larvacidal Activity of Ethanol Extracts of Phyllanthus acidus Leaves on The Culex quinquefasciatus Instar III/IV Larvae","authors":"A. Hasanah, Bagus Hermansyah, Cholis Abrori","doi":"10.19184/AMS.V5I2.6842","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.6842","url":null,"abstract":"Culex quinquefasciatus was one of a vector of filariasis disease caused by filarial worm infection. Mode of transmission was through mosquito bites that contain filaria worm. To overcome the problem in terms of eradicating the mosquito larvae was by searching for safe biological materials for humans and the environment, one of them was Phyllanthus acidus leaves. The purposes of this research were to know whether the ethanol extract of Phyllanthus acidus leaves have larvicidal activity on Culex quinquefasciatus instar III / IV larvae and to know the lethal concentration 50 (LC50) ethanol extract of Phyllanthus acidus leaves on Culex quinquefasciatus instar III / IV larvae within 24 hours. This study used true experimental method with post test only controlled group design with 1 positive control group (Temephos), 1 negative control group (Aquades and DMSO), and 5 treatment groups (0.0625%; 0.125%; 0.25%, 0.5% and 1% extract) respectively 20 Culex quinquefasciatus instar III/IV larvae. The result of regression test of inverse to know the effect of leaf extract of ceremai to the death of Culex quinquefasciatus larvae obtained R2=0909 which was mean the effect of ethanol extract of Phyllanthus acidus leaves to the number of the death of Culex quinquefasciatus larvae equal to 90.9%. The result of probit test obtained LC50 result of 0.189% with 95% confidence interval (0.142% -0.233%).","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130034009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ivan Iqbal Baidowi, Yunita Armiyanti, Zahrah Febianti, Y. Nurdian, Bagus Hermansyah
This study aimed to investigate the correlation between the use of Personal Protective Equipment (PPE) and the status of Soil-Transmitted Helminths infection in the workers of Kaliputih plantation in Jember Regency. The research method applied was Analytic observational with a Cross-sectional approach. The population, as well as the sample, were all workers of Kaliputih Plantation, Sumber Bulus Village, Ledokombo District, Jember Regency, by the total of 63 people. The sampling technique was the Total sampling. Laboratory analysis on faecal samples of respondents were conducted at the Parasitology Laboratory, Faculty of Medicine, University of Jember. Furthermore, the research data were analyzed using Chi-Square analysis or Fisher’s Exact Test. The results demonstrated that the prevalence of STH infection in Kaliputih Plantation workers was 25%. This figure was supported by the good awareness of the use of Personal Protective Equipment (PPE) of workers (91.67%). In addition, the Soil-Transmitted Helminths species that infested the plantation workers were Ascaris lumbricoides, found in 6 people (16.67%), and Hookworm, found in 3 people (8.33%). Finally, the result of Chi-square analysis showed a significance value of <0.05. Therefore, it can be concluded that the use of PPE in Kaliputih plantation workers had a significant correlation with the STH infection status. Keywords: Correlation, PPE, Soil-Transmitted Helminths Infection
{"title":"The Correlation Between The Use of Personal Protective Equipment (PPE) and Soil-Transmitted Helminths Infection in the Workers of Kaliputih Plantation Jember Regency","authors":"Ivan Iqbal Baidowi, Yunita Armiyanti, Zahrah Febianti, Y. Nurdian, Bagus Hermansyah","doi":"10.19184/AMS.V5I2.9625","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.9625","url":null,"abstract":"This study aimed to investigate the correlation between the use of Personal Protective Equipment (PPE) and the status of Soil-Transmitted Helminths infection in the workers of Kaliputih plantation in Jember Regency. The research method applied was Analytic observational with a Cross-sectional approach. The population, as well as the sample, were all workers of Kaliputih Plantation, Sumber Bulus Village, Ledokombo District, Jember Regency, by the total of 63 people. The sampling technique was the Total sampling. Laboratory analysis on faecal samples of respondents were conducted at the Parasitology Laboratory, Faculty of Medicine, University of Jember. Furthermore, the research data were analyzed using Chi-Square analysis or Fisher’s Exact Test. The results demonstrated that the prevalence of STH infection in Kaliputih Plantation workers was 25%. This figure was supported by the good awareness of the use of Personal Protective Equipment (PPE) of workers (91.67%). In addition, the Soil-Transmitted Helminths species that infested the plantation workers were Ascaris lumbricoides, found in 6 people (16.67%), and Hookworm, found in 3 people (8.33%). Finally, the result of Chi-square analysis showed a significance value of <0.05. Therefore, it can be concluded that the use of PPE in Kaliputih plantation workers had a significant correlation with the STH infection status. \u0000Keywords: Correlation, PPE, Soil-Transmitted Helminths Infection","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125416327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carpal Tunnel Syndrome frequently experienced by workers whose daily activities always use repetitive movements on the wrist. Carpal Tunnel Syndrome is a syndrome that can actually be prevented because there is no risk factor that can’t be changed except the age factor. Previous research has shown a fairly high prevalence about 42%-93% in factory workers. This study aims to determine the factors that affect the occurence of Carpal Tunnel Syndrome in women workers warehouse tobacco. This is an analytical observational study with cross sectional study design which using 50 samples who met inclusion and exclusion criteria. Instrument in this research is clinical questionnaire to diagnose CTS and Phalen test. Spearman correlation test result obtained long working p=0,036, r=0,298 and pain intensity p= <0,01, r=0,635 which means there is significant correlation with moderate strength between length of work with CTS and strong correlation between the intensity of pain with CTS. Keywords: Carpal Tunnel Syndrome, risk factor, women laborer
{"title":"Risk Factors Affecting Carpal Tunnel Syndrome in Women Laborer of Tobacco Warehouse Ajung District, Jember","authors":"Dasarina Rizqi Amalia, I. Astuti, Y. Nurdian","doi":"10.19184/AMS.V5I2.6489","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.6489","url":null,"abstract":"Carpal Tunnel Syndrome frequently experienced by workers whose daily activities always use repetitive movements on the wrist. Carpal Tunnel Syndrome is a syndrome that can actually be prevented because there is no risk factor that can’t be changed except the age factor. Previous research has shown a fairly high prevalence about 42%-93% in factory workers. This study aims to determine the factors that affect the occurence of Carpal Tunnel Syndrome in women workers warehouse tobacco. This is an analytical observational study with cross sectional study design which using 50 samples who met inclusion and exclusion criteria. Instrument in this research is clinical questionnaire to diagnose CTS and Phalen test. Spearman correlation test result obtained long working p=0,036, r=0,298 and pain intensity p= <0,01, r=0,635 which means there is significant correlation with moderate strength between length of work with CTS and strong correlation between the intensity of pain with CTS. \u0000Keywords: Carpal Tunnel Syndrome, risk factor, women laborer ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131253048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine
烧伤创面愈合过程包括炎症、增殖和重塑三个阶段。成纤维细胞的数量在增殖期增加。烧伤氧化应激可能通过抑制TGF-β破坏细胞增殖，导致成纤维细胞数量减少，烧伤创面愈合不良。毛豆种子中的染料木素具有抗炎、抗菌和抗氧化的特性。毛豆含有维生素A、C和E，它们也具有抗氧化的特性。毛豆被认为可以保护烧伤伤口免受氧化应激的影响。本研究旨在了解毛豆种子(Glycine max L. Merril)乙醇提取物(ED)对二度烧伤创面愈合成纤维细胞计数的影响。这是一项真正的实验研究，仅采用后测对照组设计。本研究的样本为24只大鼠二度烧伤模型，将热金属(60°C)附着在大鼠剃光的背部上5秒制成。将样品分为6组，分别为阳性对照组(磺胺嘧啶银)、阴性对照组(钠CMC 0.5%)、ED组20%、ED组40%、ED组60%、ED组80%。治疗15 d，第16天取皮肤标本。在400倍光镜下，对苏木精和伊红染色的组织学制剂进行拍照，观察成纤维细胞的数量。单因素方差分析(one - way ANOVA)检验，显著性值为0.011 (p≤0.05)表示两组间差异至少有统计学意义。LSD的事后分析显示，与阴性对照相比，ED 20、40和60%组有显著差异。关键词:烧伤创面愈合，蔬菜大豆，染料木素，成纤维细胞计数，磺胺嘧啶银
{"title":"Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer","authors":"Yuli Hermansyah, Ika Aulia Kurniasari, Heni Fatmawati","doi":"10.19184/AMS.V5I2.9613","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.9613","url":null,"abstract":"Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. \u0000Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114771765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regina Finka, D. Agustina, D. Rachmawati, E. Suswati, D. Mufida, A. Shodikin
Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin
{"title":"The Role of Pili Protein 38,6 kDa Klebsiella pneumoniae as a Hemagglutinin and Adhesin Protein which Serves as a Virulence Factor","authors":"Regina Finka, D. Agustina, D. Rachmawati, E. Suswati, D. Mufida, A. Shodikin","doi":"10.19184/AMS.V5I2.9558","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.9558","url":null,"abstract":"Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. \u0000Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132448462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diazinon is an organophosphate type pesticide that is still often used by farmers in Indonesia, with the effect of inhibiting the acetylcholinesterase enzyme, giving rise to the accumulation of acetylcholine in the synapse gap which will lead to incoordination, convulsions and death in insect pests. Apart from having the neurotoxic effects of diazinone it can also damage cells through the mechanism of oxidative stress. Diazinone poisoning has a high potential to cause damage to the kidney organs, because the diazinone excretion pathway and its active metabolites are through the urinary system. The purpose of this study was to determine the effect of diazinone on the liver and renal wistar male kidney. Diazinone dosage of 40 mg / kgBW, given to mice twice a day for 5 days, with each given as much as 5 ml using the gastric sonde. The research sample was in the form of rat blood taken intracardiac to examine BUN levels, serum creatinine, SGOT, SGPT, and GSH, then kidney and liver rats were taken to make histopathological preparations and MDA examinations. Analysis of this research data using the T-test for all variables. There were significant differences between groups of rats given diazinone and groups of rats not given diazinone based on levels of BUN, creatinine, SGOT, SGPT, GSH and MDA. In the group of mice not given diazinone, kidney histopathology was better than those given diazinon. Keywords: diazinon, pesticides, organophosphates
{"title":"Diazinon Toxicity to Kidney and Liver of Wistar Male Rats in terms of Biochemical and Histopathological Parameters","authors":"Desie Dwi Wisudanti, Firman Herdiana, Tegar Syaiful Qodar","doi":"10.19184/AMS.V5I2.11575","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.11575","url":null,"abstract":"Diazinon is an organophosphate type pesticide that is still often used by farmers in Indonesia, with the effect of inhibiting the acetylcholinesterase enzyme, giving rise to the accumulation of acetylcholine in the synapse gap which will lead to incoordination, convulsions and death in insect pests. Apart from having the neurotoxic effects of diazinone it can also damage cells through the mechanism of oxidative stress. Diazinone poisoning has a high potential to cause damage to the kidney organs, because the diazinone excretion pathway and its active metabolites are through the urinary system. The purpose of this study was to determine the effect of diazinone on the liver and renal wistar male kidney. Diazinone dosage of 40 mg / kgBW, given to mice twice a day for 5 days, with each given as much as 5 ml using the gastric sonde. The research sample was in the form of rat blood taken intracardiac to examine BUN levels, serum creatinine, SGOT, SGPT, and GSH, then kidney and liver rats were taken to make histopathological preparations and MDA examinations. Analysis of this research data using the T-test for all variables. There were significant differences between groups of rats given diazinone and groups of rats not given diazinone based on levels of BUN, creatinine, SGOT, SGPT, GSH and MDA. In the group of mice not given diazinone, kidney histopathology was better than those given diazinon. \u0000Keywords: diazinon, pesticides, organophosphates","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132036075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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