Aquatic Toxicology最新文献_第5页

From plankton to fish: The multifaceted threat of microplastics in freshwater environments 从浮游生物到鱼类：淡水环境中微塑料的多方面威胁

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107242

Cuimei Gao , Baohong Xu , Zhongyuan Li , Zhuoman Wang , Siqi Huang , Zijian Jiang , Xiaomin Gong , Huilin Yang

The detrimental impact of emerging pollutants, specifically microplastics (MPs), on the ecological environment are receiving increasing attention. Freshwater ecosystems serve as both repositories for terrestrial microplastic (MP) sources and conduits for their subsequent entry into marine environments. Consequently, it is imperative to rigorously investigate the toxicological effects of MPs on freshwater ecosystems. This article provides a comprehensive analysis of the ecological toxicity effects of MP pollution, both in isolation and in combination with other pollutants, on freshwater aquatic organisms, including plankton, benthic organisms, and fish. The review elucidates potential mechanisms underlying these effects, which encompass oxidative stress, metabolic disorders, immune and inflammatory responses, dysbiosis of the gut microbiota, DNA damage, and cell apoptosis. This paper advocates for the integrated application of multi-omics technologies to investigate the molecular mechanisms underlying the toxicity of MPs to freshwater aquatic organisms from interdisciplinary and multifaceted perspectives. Additionally, it emphasizes the importance of enhancing research on the compounded pollution effects arising from various pollution modes, particularly in conjunction with other pollutants. This study aims to establish a foundation for assessing the ecological risks posed by MPs in freshwater ecosystem and offers valuable insights for the protection of aquatic biodiversity and ecosystem stability.

新兴污染物特别是微塑料对生态环境的不利影响越来越受到人们的关注。淡水生态系统既是陆地微塑料（MP）来源的储存库，也是它们随后进入海洋环境的管道。因此，严格研究MPs对淡水生态系统的毒理学影响势在必行。本文全面分析了MP污染对淡水水生生物（包括浮游生物、底栖生物和鱼类）的生态毒性影响，包括单独污染和与其他污染物一起污染。这篇综述阐明了这些影响的潜在机制，包括氧化应激、代谢紊乱、免疫和炎症反应、肠道微生物群生态失调、DNA损伤和细胞凋亡。本文主张综合应用多组学技术，从跨学科、多角度研究多磺酸对淡水水生生物毒性的分子机制。此外，它强调必须加强对各种污染方式，特别是与其他污染物一起产生的复合污染效应的研究。本研究旨在为水生生物对淡水生态系统的生态风险评估奠定基础，为保护水生生物多样性和生态系统稳定性提供有价值的见解。

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引用次数: 0

Developmental and neurotoxic effects of dimethyl phthalate on zebrafish embryos and larvae 邻苯二甲酸二甲酯对斑马鱼胚胎和幼虫的发育和神经毒性作用

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107241

Weili Yang , Ruijing Li , Xingxue Yan , Pengkai Fan , Weyland Cheng , Cuihua Liu , Yaodong Zhang , Jitong Li

Dimethyl phthalate (DMP) has been extensively utilized as a plasticizer on a global scale for many years. Its presence in the environment and its harmful effects on living organisms have raised concerns. This study aimed to examine its potential developmental neurotoxicity by utilizing zebrafish as a model. Zebrafish embryos were exposed to different concentrations of DMP (5–100 mg/L) from 4 to 120 h post-fertilization (hpf). The survival, hatching, and malformation rates were recorded for each group. Behavioral analysis was conducted on zebrafish larvae, and transgenic zebrafish Tg(elavl3:EGFP) were used to assess the impact of DMP on neuronal cells. The mRNA levels of key neurological marker genes were evaluated at 96 hpf of DMP exposure. The study revealed that exposure to DMP resulted in decreased survival and hatching rates in zebrafish. Embryos treated with 50 mg/L of DMP exhibited lower average survival rates (72.78–78.33%) between 24–96 hpf, while treatment with 25–50 mg/L of DMP resulted in reduced hatching rates (39.44% and 2.22%, respectively) at 48 hpf compared to the control group. Moreover, exposure to 25–50 mg/L of DMP caused an increase in malformations, such as tail curvature, spinal curvature, yolk sac edema and pericardial edema. Interestingly, at 24 hpf, DMP also resulted in an increase in spontaneous tail coiling in zebrafish embryos, as well as a decrease in their swimming distance at 120 hpf. Furthermore, treatment with 50 mg/L of DMP led to a decrease in the fluorescence intensity of transgenic zebrafish Tg(elavl3: EGFP). RT-qPCR analysis showed a significant down-regulation of marker genes (gap43, mbp, α1-tubulin, syn2a) associated with nervous system function in DMP-treated zebrafish. Overall, these findings offer a thorough understanding of the mechanisms underlying the neurotoxicity caused by DMP, highlighting the risk of DMP on developmental and neurotoxic effects in zebrafish. Therefore, strict supervision of DMP use and release is essential to safeguard ecological and aquatic organisms.

邻苯二甲酸二甲酯（DMP）作为增塑剂在全球范围内得到了广泛的应用。它在环境中的存在及其对生物体的有害影响引起了人们的关注。本研究旨在以斑马鱼为模型，研究其潜在的发育神经毒性。将斑马鱼胚胎在受精后4 ~ 120 h暴露于不同浓度的DMP （5 ~ 100 mg/L）中。记录各组成活率、孵化率和畸形率。对斑马鱼幼鱼进行行为分析，采用转基因斑马鱼Tg（elavl3:EGFP）评价DMP对神经元细胞的影响。在DMP暴露96 hpf时评估关键神经学标记基因的mRNA水平。研究表明，接触DMP会导致斑马鱼的存活率和孵化率下降。50 mg/L DMP处理的胚胎在24 ~ 96 hpf期间的平均存活率较低（72.78 ~ 78.33%），而25 ~ 50 mg/L DMP处理的胚胎在48 hpf时的孵化率较对照组降低（分别为39.44%和2.22%）。此外，暴露于25-50 mg/L的DMP导致畸形增加，如尾弯曲、脊柱弯曲、卵黄囊水肿和心包水肿。有趣的是，在24 hpf时，DMP也导致斑马鱼胚胎自发尾卷的增加，以及在120 hpf时游泳距离的减少。此外，50mg /L的DMP处理导致转基因斑马鱼Tg（elavl3: EGFP）的荧光强度降低。RT-qPCR分析显示，dmp处理的斑马鱼神经系统功能相关的标记基因（gap43、mbp、α1-微管蛋白、syn2a）显著下调。总的来说，这些发现提供了对DMP引起神经毒性的机制的透彻理解，强调了DMP对斑马鱼发育和神经毒性作用的风险。因此，严格监管DMP的使用和释放对保护生态和水生生物至关重要。

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引用次数: 0

Harmful effects of the semiconductor copper tungstate (CuWO4) on multiple photosynthetic parameters of the green microalga Raphidocelis subcapitata 半导体钨酸铜（CuWO4）对绿微藻Raphidocelis subcapitata多个光合参数的有害影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107245

Renan Castelhano Gebara , Cínthia Bruno de Abreu , Giseli Swerts Rocha , Adrislaine da Silva Mansano , Marcelo Assis , Ailton José Moreira , Mykaelli Andrade Santos , Thalles Maranesi Pereira , Luciano Sindra Virtuoso , Maria da Graça Gama Melão , Elson Longo

The semiconductor copper tungstate (CuWO₄) may end up in aquatic ecosystems since it has the potential for water decontamination. The toxic effects of CuWO₄ are totally unknown for eukaryotic organisms. In view of this, we aimed to evaluate the toxicity of CuWO₄ particles (size of 161.5 nm) on the cosmopolitan green microalga Raphidocelis subcapitata (a standardized test-organism for ecotoxicological assays), analyzing the growth and multiple photosynthetic parameters obtained by pulse amplitude modulated (PAM) fluorometry. At 1.2 mg l^-1, the growth was affected, and there was an increase in reactive oxygen species (ROS) after 72 h The effective efficiency (ɸ_M’) of photosystem II (PSII) was affected at 13.1 mg l^-1, while the efficiency of the oxygen-evolving complex (OEC), responsible for the water-splitting process, decayed at 5.6 mg l^-1. According to quenching parameters, energy allocated to photosynthetic processes (qP) decreased, indicating a malfunctioning of the PSII. We also observed a 50 % increase in the non-regulated energy dissipation by heat and fluorescence (Y(NO)), and a 50 % decrease in the regulated energy dissipation (NPQ), suggesting difficulties for algae to cope with light. Rapid light curves (RLC) were the second most sensitive parameter, as we observed a decay of the relative maximum electron transport rate (rETRmax) at 2.8 mg l^-1. Therefore, since the microalga R. subcapitata was affected by concentrations up to 1.2 mg l^-1 (0.01 mg l^-1 dissolved Cu), it is important to evaluate carefully the use of CuWO₄ to decontaminate natural waters, considering the protection of the aquatic biota.

半导体钨酸铜（CuWO4）可能最终进入水生生态系统，因为它具有水净化的潜力。CuWO4对真核生物的毒性作用是完全未知的。鉴于此，我们旨在通过分析脉冲振幅调制（PAM）荧光法获得的生长和多种光合参数，评估CuWO4颗粒（尺寸为161.5 nm）对世界性绿色微藻Raphidocelis subcapitata（生态毒理学试验标准化试验生物）的毒性。当浓度为1.2 mg l-1时，藻体生长受到影响，72 h后活性氧（ROS）增加；当浓度为13.1 mg l-1时，光系统II （PSII）的有效效率（h M′）受到影响，而负责水分解过程的出氧复合体（OEC）的效率在5.6 mg l-1时下降。根据猝灭参数，分配给光合过程的能量（qP）减少，表明PSII出现故障。我们还观察到，由热和荧光引起的非调节能量耗散（Y(NO)）增加了50%，而调节能量耗散（NPQ）减少了50%，这表明藻类在应对光方面存在困难。快速光曲线（RLC）是第二敏感的参数，因为我们观察到相对最大电子传输速率（rETRmax）在2.8 mg l-1时衰减。因此，由于小头圆藻受到高达1.2 mg l-1 （0.01 mg l-1溶解Cu）浓度的影响，考虑到水生生物群的保护，仔细评估CuWO4在自然水体中的净化作用是很重要的。

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引用次数: 0

Assessing spinel zinc ferrite nanoparticles in aquatic ecosystems: Toxic threat or beneficial detoxifier for aquatic life? 评估水生生态系统中的尖晶石铁酸锌纳米颗粒：对水生生物的有毒威胁还是有益的解毒剂？

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107218

Selin Ertürk Gürkan , Mert Gürkan , Ece Büşra Yanik , Elif Kutlu , Volkan Saritunç , Berkay Güneş , Ezgi Can İbiş

The effectiveness of magnetic nanoparticles in removing pollutants during water treatment is well established, but their introduction into aquatic ecosystems raises significant toxicity concerns. This study investigates the histological and physiological effects of zinc ferrite magnetic nanoparticles (ZnFe₂O₄₋MNPs) on the Mediterranean mussel (Mytilus galloprovincialis) and examines the impact of concurrent exposure to these nanoparticles and the insecticide thiomethoxam (TMX).

Mussels were exposed to nominal concentrations of ZnFe₂O₄₋MNPs (1, 10, 100 mg/L) both individually and with TMX. Physiological assessments included measuring antioxidant enzyme levels (superoxide dismutase, catalase, glutathione S-transferase) and oxidative stress markers (malondialdehyde). Results showed that ZnFe₂O₄₋MNPs increased antioxidant activity but also caused dose-dependent pathological changes. In contrast, combined exposure with TMX significantly (p < 0.05) reduced antioxidant defenses, indicated by lower superoxide dismutase (SOD) levels and higher malondialdehyde (MDA) concentrations, suggesting oxidative stress and potential cellular damage.

These findings highlight the need for comprehensive toxicity assessments of nanoparticles in aquatic environments and advocate for their complete removal from water sources post-treatment. Further research is crucial to define the toxicity profiles of spinel ferrites to ensure their safe application in environmental remediation.

磁性纳米颗粒在水处理过程中去除污染物的有效性已得到充分证实，但将其引入水生生态系统引起了严重的毒性问题。本研究研究了铁氧体锌磁性纳米颗粒（ZnFe2O4−MNPs）对地中海贻贝（Mytilus galloprovincialis）的组织学和生理影响，并研究了同时暴露于这些纳米颗粒和杀虫剂硫虫嗪（TMX）的影响。

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引用次数: 0

An effective method for distinguishing extracellular and intracellular nanoparticles through chemical extractions

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107264

Bin Huang , Jie-Yu Chang , Bo-Wen Li , Lu He , Rui-Si Liang , Huan He , Ai-Jun Miao , Hui Li

Nanoparticles (NPs) can adsorb onto cell surfaces (i.e., extracellular NPs) and be internalized by cells (i.e., intracellular NPs), leading to their accumulation and potential toxicity. Therefore, distinguishing adsorbed and internalized NPs is crucial in studying their accumulation and toxicity. In this study, various washing agents (EDTA, sodium citrate, cysteine, and H⁺) were compared to differentiate internalized NPs from total accumulation in the protozoan Tetrahymena thermophila, by evaluating efficacies of extracting adsorbed NPs from cell surfaces. Key factors influencing the extraction procedures, including the type and concentration of washing agent, contact time, washing cycles, and effects of agents on the organism, were systematically optimized. Consequently, we identified an effective washing agent (i.e., a mixture of 3 mM EDTA, 10 mM sodium citrate, and 10 mM cysteine in Dryl's medium at pH 7) that efficiently extracted adsorbed metal NPs (Fe₂O₃-NPs, TiO₂-NPs, SiO₂-NPs, Ag-NPs, and Au-NPs modified by -COOH and -NH₂) without causing growth inhibition or cell lysis. Further, a washing procedure was proposed, involving the extraction of samples with the mixture twice for 5 min each. Our study represents the first systematic optimization of a washing protocol for extracting adsorbed NPs across diverse NP types. The developed methodology demonstrates broad applicability, minimal impact on cellular function, and enhanced extraction efficiency compared to existing methods. It will facilitate further investigation into the underlying mechanisms of NP bioaccumulation (including uptake and efflux) and associated toxicity in protozoa, providing critical insights for environmental safety assessments and advancing nanotoxicology research.

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引用次数: 0

Metal concentrations, oxidative status and histopathological evaluation of fish species from Doce River, Brazil, after the Fundao dam collapse Fundao大坝崩塌后巴西多塞河鱼类金属浓度、氧化状态及组织病理学评价

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107204

Renata M.P. Freitas , Allan R. Pires , Federico F. Ferreira , Emerson F. Vilela , Filipe S. Azevedo , Mariáurea M. Sarandy , Reggiani V. Gonçalves , Jorge A. Dergam , Carlos F. Sperber , Mariella B. Freitas

Eight years after the tailings dam collapse in Mariana, MG, Brazil, several aspects of this massive disaster are yet to be elucidated. Our goal was to investigate the impact of the mud flow on 16 fish species collected across 15 points from the Doce River, addressing 12 metal concentrations, tissue oxidative status (antioxidant enzymes and stress biomarkers), and histopathological analyses. The species Trachelyopterus striatulus, Prochilodus vimboides, Loricariichthys castaneus, Lophiosilurus alexandri, Hypostomus affinis, Hoplias intermedius were shown to be the most affected regarding the gills tissue integrity; Hypostomus affinis, Oligosarcus acutirostris, Lophiosilurus alexandri, Pygocentrus nattereri, Hoplosternum littorale, and Loricariichthys castaneus showed the highest levels of liver health. Overall, H. affinis was the most affected species, showing high levels of oxidative and histopathological damage, associated with high arsenic (As) and mercury (Hg) concentrations. In fish sampled from impacted regions, As and Hg exhibited higher concentrations compared to fish from unaffected sites, surpassing all the other analyzed metals. These high metal concentrations might be associated to the tailings dam failure, and As and Hg concentrations were positively correlated with alterations in oxidative stress biomarkers and histopathologies. Our results may be used as baseline for monitoring the environmental challenges that the Doce River fish species are facing at the moment.

在巴西MG州马里亚纳的尾矿坝坍塌八年后，这场巨大灾难的几个方面仍有待阐明。我们的目标是调查泥石流对Doce河15个点采集的16种鱼类的影响，解决12种金属浓度、组织氧化状态（抗氧化酶和应激生物标志物）和组织病理学分析。纹状斑尾蛾（Trachelyopterus striatulus）、斑尾蛾（Prochilodus vimboides）、castaneus、Lophiosilurus alexandri、Hypostomus affinis、Hoplias intermedius鳃组织完整性受影响最大；affinus、Oligosarcus acutirostris、Lophiosilurus alexandri、Pygocentrus nattereri、Hoplosternum littorale和Loricariichthys castaneus的肝脏健康水平最高。总体而言，麻蝇是受影响最严重的物种，表现出高水平的氧化和组织病理学损伤，与高砷（As）和汞（Hg）浓度有关。在受影响地区的鱼类样本中，砷和汞的浓度高于未受影响地区的鱼类，超过了所有其他分析的金属。砷和汞浓度与氧化应激生物标志物和组织病理学变化呈正相关。我们的研究结果可以作为监测多色河鱼类目前面临的环境挑战的基线。

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引用次数: 0

Extremely low repeated pyrethroid pulses increase harmful effects on caddisfly larvae (Chaetopteryx villosa) and influence species interactions 极低的重复拟除虫菊酯脉冲增加了对毛翅虫幼虫（绒毛毛翅鸟）的有害影响，并影响物种相互作用。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107212

Sarah Betz-Koch , Jörg Oehlmann , Matthias Oetken

Based on effect data, regulatory acceptable concentrations (RACs) are derived for surface waters to avoid unacceptable effects on the environment. RACs often relay on acute tests with single species, which may underestimate the effects under field conditions. Therefore, we applied a higher tier approach with artificial indoor streams (AIS). We exposed representatives of the benthic community in lotic surface waters to varying numbers (one to four times) of 12-hour deltamethrin pulses over a 35-day period with intervening recovery phases, to simulate multiple pesticide peak exposures caused by rain events or spray drift. The deltamethrin concentration of each pulse was equivalent to its RAC value of 0.64 ng/L and consequently should have no unacceptable effects on the tested species.

In contrast, we observed that the mortality of caddisfly larvae increased significantly with the number of pulses at the RAC. In addition, larval development was significantly delayed after four deltamethrin pulses, while the gammarids apparently benefited from the toxicity-induced mortality of the larvae.

This study underlines the importance of considering higher tier approaches that include species interactions and additional stressors in order to obtain more realistic effect data and optimise regulatory risk assessment. These are not considered in acute tests with single species, which usually leads to an underestimation of the effects. Based on the results of this study, we propose to lower the RAC value for aquatic environments due to the uncertainties mentioned above.

根据影响数据，为避免对环境产生不可接受的影响，得出了地表水的监管可接受浓度。rac通常依赖于单一物种的急性试验，这可能低估了在野外条件下的影响。因此，我们采用了更高层次的方法，即人工室内溪流（AIS）。我们在35天的间歇恢复阶段，将lotic地表水底栖生物群落的代表暴露在不同次数（1至4次）的12小时溴氰菊酯脉冲中，以模拟由降雨事件或喷雾漂移引起的多重农药峰值暴露。每个脉冲的溴氰菊酯浓度相当于其RAC值0.64 ng/L，因此对被试物种应该没有不可接受的影响。相反，我们观察到，随着脉冲次数的增加，球蛾幼虫的死亡率显著增加。此外，4次溴氰菊酯脉冲处理显著延缓了幼虫的发育，而伽马雌虫则明显受益于毒性诱导的幼虫死亡。这项研究强调了考虑更高层次方法的重要性，包括物种相互作用和额外的压力源，以获得更现实的效果数据和优化监管风险评估。在单一物种的急性试验中不考虑这些因素，这通常会导致对影响的低估。基于本研究的结果，由于上述不确定性，我们建议降低水生环境的RAC值。

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引用次数: 0

TBBPA caused multiple intestinal injuries via ROS/NF-κB signal in common carp TBBPA通过ROS/NF-κB信号引起鲤鱼多处肠道损伤。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107190

Man Qian, Yuan Geng, Jing-jing Wang, Hong-ru Wang, Ji-long Luo, Xue-jiao Gao

Tetrabromobisphenol A (TBBPA) is an aquatic environment's prevalent pollutant, posing a great threat to the health of aquatic animals. The intestine is a key organ for nutrient absorption as well as an important barrier to prevent pollutants from invading the body of fish. Exploring the effects of pollutants on the intestine is of great significance for maintaining fish health. Therefore, the purpose of this study was to assess the toxic effects of TBBPA on the intestine of Cyprinus carpio L. (common carp) by establishing models of common carp and primary intestinal epithelial cells exposed to TBBPA. Histological observation revealed that TBBPA exposure led to damage in the intestinal mucosa and breakage of intestinal villi. Detection of oxidative stress levels showed that TBBPA increased the levels of ROS and MDA, and decreased the activity of SOD, CAT, GSH-PX, and T-AOC in intestinal tissue and cells. Observation of inflammatory factor levels revealed that TBBPA upregulated the mRNA levels of inflammatory factors (IL-6, TNF-α, IL-1β, NF-κB p65 and IκBα). ELISA and western blotting results were consistent with the mRNA results. Moreover, TBBPA induced cell death, as evidenced by TUNEL staining and flow cytometry and confirmed by increasing levels of Bax, Cas-3, Cyt C, RIP1, RIP3, and MLKL, together with decreasing the levels of Bcl-2. TBBPA also destroyed the intestinal tight junction by reducing the mRNA and protein levels of claudin-1, ZO-1, and occludin. In summary, this study reveals that TBBPA caused intestinal injuries, inducing oxidative stress, inflammation, cell death, and tight junction disruption via ROS/NF-κB signal in common carp.

四溴双酚A （TBBPA）是水生环境中普遍存在的污染物，对水生动物的健康构成极大威胁。肠道是营养吸收的重要器官，也是防止污染物侵入鱼类身体的重要屏障。探讨污染物对肠道的影响对维持鱼类健康具有重要意义。因此，本研究的目的是通过建立暴露于TBBPA的鲤鱼和原代肠上皮细胞模型，来评估TBBPA对鲤鱼肠道的毒性作用。组织学观察显示，TBBPA暴露导致肠黏膜损伤和肠绒毛断裂。氧化应激水平检测显示，TBBPA增加了肠道组织和细胞中ROS和MDA的水平，降低了SOD、CAT、GSH-PX和T-AOC的活性。观察炎症因子水平发现，TBBPA上调炎症因子（IL-6、TNF-α、IL-1β、NF-κB p65、i -κB α） mRNA水平。ELISA和western blotting结果与mRNA结果一致。TUNEL染色和流式细胞术证实，TBBPA诱导细胞死亡，Bax、cas3、Cyt C、RIP1、RIP3、MLKL水平升高，Bcl-2水平降低。TBBPA还通过降低claudin-1、ZO-1和occludin的mRNA和蛋白水平破坏肠紧密连接。综上所述，本研究揭示了TBBPA通过ROS/NF-κB信号引起鲤鱼肠道损伤，诱导氧化应激、炎症、细胞死亡和紧密连接破坏。

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引用次数: 0

Biotransport and toxic effects of micro- and nanoplastics in fish model and their potential risk to humans: A review 微塑料和纳米塑料在鱼类模型中的生物运输和毒性作用及其对人类的潜在风险综述。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107215

Yanan Xu , Ling Liu , Yuqing Ma , Cunlong Wang , Fengshang Duan , Jianxue Feng , Haiyang Yin , Le Sun , Zhihan Cao , Jinho Jung , Ping Li , Zhi-Hua Li

The growing body of scientific evidence suggests that micro- and nanoplastics (MPs/NPs) pose a significant threat to aquatic ecosystems and human health. These particles can enter organisms through ingestion, inhalation, dermal contact, and trophic transfer. Exposure can directly affect multiple organs and systems (respiratory, digestive, neurological, reproductive, urinary, cardiovascular) and activate extensive intracellular signaling, inducing cytotoxicity involving mechanisms such as membrane disruption, extracellular polymer degradation, reactive oxygen species (ROS) production, DNA damage, cellular pore blockage, lysosomal instability, and mitochondrial depolarization. This review focuses on current research examining the in vivo and in vitro toxic effects of MPs/NPs on aquatic organisms, particularly fish, in relation to particulate toxicity aspects (such as particle transport mechanisms and structural modifications). Meanwhile, from the perspectives of the food chain and environmental factors, it emphasizes the comprehensive threats of MPs/NPs to human health in terms of both direct and indirect toxicity. Additionally, future research needs and strategies are discussed to aid in mitigating the potential risks of particulate plastics as carriers of toxic trace elements to human health.

越来越多的科学证据表明，微塑料和纳米塑料对水生生态系统和人类健康构成重大威胁。这些颗粒可通过摄入、吸入、皮肤接触和营养转移进入生物体。暴露可直接影响多个器官和系统（呼吸、消化、神经、生殖、泌尿、心血管），并激活广泛的细胞内信号，诱导细胞毒性，包括膜破坏、细胞外聚合物降解、活性氧（ROS）产生、DNA损伤、细胞孔堵塞、溶酶体不稳定和线粒体去极化等机制。本文综述了目前研究MPs/NPs对水生生物，特别是鱼类的体内和体外毒性作用，涉及颗粒毒性方面（如颗粒运输机制和结构修饰）。同时，从食物链和环境因素的角度，强调了MPs/NPs对人类健康的直接毒性和间接毒性的综合威胁。此外，还讨论了未来的研究需求和策略，以帮助减轻颗粒塑料作为有毒微量元素载体对人类健康的潜在风险。

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引用次数: 0

Assessment of the effects of cadmium, samarium and gadolinium on the blue mussel (Mytilus edulis): A biochemical, transcriptomic and metabolomic approach 镉、钐和钆对蓝贻贝（Mytilus edulis）影响的评估：生化、转录组学和代谢组学方法。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107217

Binbin CAI , Laura GANDON , Clément BARATANGE , Oluwabunmi ELEYELE , Romaric MONCRIEFFE , Grégory MONTIEL , Abderrahmane KAMARI , Samuel BERTRAND , Marie-José DURAND , Laurence POIRIER , Paul DELERIS , Aurore ZALOUK-VERGNOUX

Improving the understanding of how chemicals affect on organisms and assessing the associated environmental risks is of major interest in environmental studies. This can be achieved by using complementary approaches based on the study of the molecular responses of organisms. Because of the known chemical pressures on the environment, regulations on the content of some chemicals, such as cadmium, have been mostly completed. In contrast, the environmental toxicity of rare earth elements (REEs), which are widely used in industry, has only recently begun to receive attention. Here, we investigated the effects of cadmium, and two REEs, samarium and gadolinium, on marine mussels under laboratory exposures. We found that after an 8-day exposure at 500 µg/L, the metals were bioaccumulated by the mussels. Furthermore, samarium and gadolinium affected two oxidative stress biomarkers, GST and SOD. Lipidomic analysis showed that lipid content was modulated by the REEs, but not by cadmium. Interestingly, several compounds belonging to the phosphoinositide metabolism were more abundant, suggesting a pro-mitotic or cell survival response, while a higher abundance of cardiolipins after samarium exposure suggested an alteration of mitochondrial activity. Moreover, depending on the tissue and the metal considered, transcriptional analyses revealed an effect on metallothionein, hsp70/90, energy metabolism enzymes, as well as pro-mitotic transcript accumulation. Thus, this study sheds a new light on metal toxicity and in particularl REEs by highlighting the accumulation and toxicity of cadmium, samarium and gadolinium at 500 µg/L at different molecular levels, from gene expression to the lipidome of blue mussels.

提高对化学品如何影响生物体的理解和评估相关的环境风险是环境研究的主要兴趣。这可以通过利用基于生物分子反应研究的互补方法来实现。由于已知的化学品对环境的压力，对某些化学品（如镉）含量的规定已基本完成。相反，工业上广泛使用的稀土元素（ree）的环境毒性直到最近才开始受到重视。在这里，我们研究了镉和两种稀土元素，钐和钆在实验室暴露下对海洋贻贝的影响。我们发现，在500µg/L的浓度下暴露8天后，这些金属被贻贝生物积累。此外，钐和钆还会影响GST和SOD这两个氧化应激生物标志物。脂质组学分析表明，稀土能调节脂肪含量，而镉不能调节脂肪含量。有趣的是，一些属于磷酸肌苷代谢的化合物更丰富，这表明有丝分裂或细胞存活反应，而钐暴露后心磷脂的丰度更高，表明线粒体活性发生了改变。此外，根据所考虑的组织和金属，转录分析揭示了对金属硫蛋白、hsp70/90、能量代谢酶以及促有丝分裂转录物积累的影响。因此，本研究通过强调500µg/L镉、钐和钆在不同分子水平上的积累和毒性，从基因表达到蓝贻贝的脂质组，为金属毒性特别是稀土的研究提供了新的思路。

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