Aquatic Toxicology最新文献_第4页

The diving beetle, Cybister lateralimarginalis (De Geer, 1774), as a bioindicator for subcellular changes affected by heavy metal(loid) pollution in freshwater ecosystems

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107258

Dora Bjedov , Nataša Turić , Alma Mikuška , Goran Vignjević , Lucija Sara Kovačić , Ana Marija Pavičić , Leontina Toth Jakeljić , Mirna Velki

The present study assessed Cybister lateralimarginalis as a model species for ecotoxicological studies of metal(loid) bioaccumulation in freshwater ecosystems, by optimising protocols and analysing biomarker activities and metal(loid) concentrations from different sites and investigating their association. To accomplish this, levels of arsenic (As), selenium (Se), cadmium (Cd), mercury (Hg), and lead (Pb) were analysed, as well as a set of biomarkers were evaluated, esterase activity and oxidative stress biomarkers: fluorescence- and glutathione-based. C. lateralimarginalis were sampled in the spring/summer of 2023 along the Sava, Drava and Danube floodplains in Croatia. C. lateralimarginalis samples from Sava floodplain (Mužilovčica) showed altered carboxylesterase (CES) activity, lower glutathione (GSH) levels, and elevated Hg and As concentrations. The Weighted Pollution Index (WPI) indicated moderate pollution at the Sava floodplain, possibly due to agricultural or petrochemical impacts. At the Drava floodplain (Podravlje), higher glutathione S-transferase (GST) activity and elevated Cd, Hg, and As concentrations were observed, likely from soil accumulation, while Stara Drava beetles had lower GSH levels, suggesting agricultural impacts. Despite this, the Drava floodplain showed the lowest WPI. Higher GST activity was noted at the Danube floodplain (Kopački rit and Topoljski Dunavac), with reactive oxygen species (ROS) concentrations (Kopački rit and Podunavlje) possibly reflecting agricultural practices or illegal hunting. The present research successfully applied C. lateralimarginalis as a bioindicator species for assessing metal(loid)s in different environments, highlighting its reliability as an abundant apex predator, emphasizing the significance of integrating biomarker response evaluation into pollutant monitoring for efficient environmental risk assessment in freshwater ecosystems.

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引用次数: 0

Acute and chronic toxicity of cationic polyquaterniums of varying charge density and molecular weight to Daphnia magna and Ceriodaphnia dubia 不同电荷密度和分子量的阳离子聚季铵盐对大水蚤和小水蚤的急性和慢性毒性。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107178

Jessica L. Brill , Anna Magdalene Brun Hansen , Hope Woods , Kristin A. Connors , Hans Sanderson , Scott E. Belanger

Cationic polymers have the unique ability to neutralize negative charge with practical applications in personal care products, such as shampoos, conditioners, contact lens solutions, and as flocculants in wastewater treatment processes. Cationic polymers are a diverse class of materials varying in structural composition, cationic charge density (CD), and molecular weight (MW). In this study, we investigated three classes of polyquaternium cationic polymers (PQ-6, PQ-10, PQ-16) of varying CD and MW to characterize their toxicity to aquatic invertebrates. Although safety studies and environmental risk assessments have been conducted to support the use of polyquaterniums, further research was needed to adapt standard toxicity assays to account for the unique properties of cationic polymers. Standard acute OECD 202 assays were conducted with Daphnia magna and Ceriodaphnia dubia to explore relative species sensitivity. PQ-6 exhibited toxicity ranging from 0.09 to 2.50 mg/L, PQ-10 ranged from 21.29 to >1000 mg/L, and PQ-16 ranged from 0.05 to 14.91 mg/L. Toxicity was positively correlated with CD and not correlated with MW or polymeric backbone. C. dubia were more sensitive to PQ exposure than D. magna, and this trend was consistent across all exposures. Organic carbon (humic acid and algae) was found to mitigate PQ toxicity in a dose-dependent manner, highlighting the importance of robust exposure characterization when assessing environmental risk. Chronic reproductive toxicity assays were conducted on high and low MW PQ-6 materials with observed significant decreases in days to first clutch, average offspring per clutch, and body length. Robust acute to chronic (ACR) values were derived.

阳离子聚合物具有中和负电荷的独特能力，可实际应用于洗发水、护发素、隐形眼镜溶液等个人护理产品，以及废水处理过程中的絮凝剂。阳离子聚合物是一类种类繁多的材料，其结构组成、阳离子电荷密度（CD）和分子量（MW）各不相同。在本研究中，我们研究了三类不同 CD 和 MW 的聚季铵盐阳离子聚合物（PQ-6、PQ-10、PQ-16），以确定它们对水生无脊椎动物的毒性。尽管已经开展了安全研究和环境风险评估来支持聚季铵盐的使用，但仍需进一步研究如何调整标准毒性检测方法，以考虑阳离子聚合物的独特性质。用大型蚤和糠虾进行了标准的急性 OECD 202 试验，以探索物种的相对敏感性。PQ-6 的毒性范围为 0.09 至 2.50 毫克/升，PQ-10 的毒性范围为 21.29 至大于 1000 毫克/升，PQ-16 的毒性范围为 0.05 至 14.91 毫克/升。毒性与 CD 呈正相关，与 MW 或聚合物骨架无关。杜父鱼对 PQ 暴露的敏感性高于大型蜗牛，而且这种趋势在所有暴露中都是一致的。有机碳（腐殖酸和藻类）以剂量依赖性的方式减轻了 PQ 的毒性，这突出了在评估环境风险时进行可靠的暴露特征描述的重要性。对高和低 MW PQ-6 材料进行了慢性生殖毒性试验，观察到第一窝产仔天数、每窝平均产仔数和体长显著下降。得出了可靠的急性至慢性（ACR）值。

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引用次数: 0

The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna 奥利司他破坏大水蚤能量稳态和繁殖的有害作用及其机制。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107201

Zhihua Ni , Xinling Tian , Wenbo Zhao , Wenkai Hu , Jinghua Lv , Xiaoli Sun , Yajie Zhang , Yiwen Zhang , Yuming Zhang , Baoku Li , Fengsong Liu

Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL. Here, the effects of ORL on Daphnia magna were evaluated using a 21-day chronic test at concentrations of 1, 10, 100, and 1000 μg/L. We found the inhibition of feeding and swimming activities in the 100 and 1000 μg/L ORL exposed D. magna, respectively. Their digestive enzyme activities and metabolites were reduced even at 1 μg/L ORL exposure. It is noteworthy that exposure to 100 μg/L ORL induced a decrease in the reproductive capacity of D. magna, although no discernible genotoxicity was observed. To identify the toxicological mechanisms of ORL, a metabolic analysis was conducted on D. magna exposed to 1000 μg/L ORL. A comprehensive reduction in carbohydrates, lipids, and amino acids was observed, which resulted in a blockage of metabolic flux towards the TCA cycle, as evidenced by mitochondrial dysfunction. These findings substantiate the detrimental impact of ORL on D. magna and provide insights into the underlying molecular mechanisms from a metabolic perspective.

奥利司他（Orlistat， ORL）作为一种抗肥胖药物已经使用了几十年。由于吸收率较低，大部分口服ORL随粪便排出到环境中。收集与ORL有关的可能生态风险的科学信息至关重要。本研究采用1、10、100和1000 μg/L浓度下的21天慢性试验来评估ORL对大水蚤的影响。我们发现，暴露于100 μg/L和1000 μg/L ORL的D. magna分别抑制了摄食和游泳活动。即使在1 μg/L的ORL暴露下，它们的消化酶活性和代谢物也有所降低。值得注意的是，暴露于100 μg/L的ORL可导致大鼠的生殖能力下降，但未观察到明显的遗传毒性。为了确定ORL的毒理学机制，我们对暴露于1000 μg/L ORL的大鼠进行了代谢分析。观察到碳水化合物、脂质和氨基酸的全面减少，导致向TCA循环的代谢通量受阻，线粒体功能障碍就是证据。这些发现证实了ORL对D. magna的有害影响，并从代谢的角度为潜在的分子机制提供了见解。

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引用次数: 0

Characterization of acetylcholinesterase and carboxylesterases in the mangrove oyster Crassostrea gasar as biomarkers of exposure to environmental pollutants 红树林牡蛎长牡蛎乙酰胆碱酯酶和羧酸酯酶作为环境污染物暴露的生物标志物

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107225

Karim H. Lüchmann , Bárbara P.H. Righetto , Daína Lima , Hortência C. Luz , Clarissa P. Ferreira , Bárbara H. Schallenberger , Carlos H.A.M. Gomes , Afonso C.D. Bainy

Brazil is one of the world's leading consumers of agricultural pesticides, highlighting the urgent need to identify responsive biomarkers as diagnostic and prognostic tools for monitoring aquatic pollution. Acetylcholinesterase (AChE) and carboxylesterases (CbE) are B-esterases enzymes expressed in several organisms. AChE plays an essential role in neural transmission at cholinergic synapses, while CbE are directly involved in the detoxification of organic pollutants, including organophosphorus pesticides. The activities of AChE and CbE in bivalves have not been extensively investigated, despite their suitability as sentinel organisms for environmental monitoring. In this study, we characterized the activities of AChE and CbE in the mangrove oyster Crassostrea gasar, collected from an estuarine system in southern Brazil. We compared enzymatic activities between the gills and the digestive gland, revealing that CbE activity was significantly higher in the digestive gland, while AChE activity did not differ between the two tissues. These results indicate that the digestive gland functions as the primary metabolic organ in C. gasar. Additionally, we observed notable differences in CbE activity depending on the substrate used: ρ-nitrophenyl acetate (ρNPA), ρ-nitrophenyl butyrate (ρNPB), α-naphtyl acetate (αNA), and α-naphtyl butyrate (αNB). Our findings suggest that more lipophilic substrates are metabolized more rapidly in both the digestive gland and gills. These results enhance our understanding of the biotransformation processes and neurotoxicity potential of pesticides in oysters. However, further in vitro validation is needed to confirm the utility of these biomarkers for monitoring environmental pollution in coastal waters.

巴西是世界上主要的农业农药消费国之一，这突出表明迫切需要确定反应性生物标志物，作为监测水生污染的诊断和预后工具。乙酰胆碱酯酶（AChE）和羧酸酯酶（CbE）是几种生物中表达的b -酯酶。AChE在胆碱能突触的神经传递中起着至关重要的作用，而CbE则直接参与有机污染物的解毒，包括有机磷农药。AChE和CbE在双壳类动物中的活性尚未被广泛研究，尽管它们适合作为环境监测的哨兵生物。在这项研究中，我们对采集自巴西南部河口系统的红树牡蛎（Crassostrea gasar）中AChE和CbE的活性进行了表征。我们比较了鳃和消化腺的酶活性，发现消化腺的CbE活性明显更高，而AChE活性在两种组织之间没有差异。这些结果表明，消化腺是棘豆的主要代谢器官。此外，我们观察到CbE活性的显著差异取决于所使用的底物：ρ-乙酸硝基苯酯（ρNPA）， ρ-丁酸硝基苯酯（ρNPB）， α-乙酸萘酯（αNA）和α-丁酸萘酯（αNB）。我们的研究结果表明，在消化腺和鳃中，更多的亲脂性底物代谢更快。这些结果增强了我们对农药在牡蛎中的生物转化过程和神经毒性潜力的理解。然而，需要进一步的体外验证来确认这些生物标志物在监测沿海水域环境污染方面的效用。

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引用次数: 0

Alteration of intestinal microbiota-intestinal barrier interaction interferes with intestinal health after microcystin-LR exposure in Lithobates catesbeianus tadpoles 微囊藻毒素- lr暴露后，微囊藻毒素- lr对蝌蚪肠道健康的影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107249

Jun He , Fengqi Zhang , Minglan Fang , Yuchen Zhang , Changjing Zhu , Shangfei Xiang , Desheng Yu , Hailong Wu , Yilin Shu

There remains uncertainty regarding the influence of microcystin-leucine arginine (MC-LR) on amphibian intestinal health, specifically how MC-LR interferes with intestinal microbiota following exposure to environmental concentrations. In this study, Lithobates catesbeianus tadpoles were exposed to varying MC-LR concentrations (0, 0.5, and 2 µg/L) over a 30-day period. The aim was to investigate how altered interactions between tadpole intestinal microbiota and the intestinal barrier influence intestinal health following MC-LR exposure. Following exposure to the MC-LR at low ambient concentrations, tadpole intestinal tissue was damaged. It had increased permeability, reduced pathogen inhibition capacity, and impaired digestive function. Additionally, there was a significant increase in lipopolysaccharide content and upregulation of downstream response genes, including TLR4, MyD88, and NF-κB, within the intestinal tissue. Therefore, eosinophils’ count and pro-inflammatory cytokines’ expression increased. In addition, MC-LR exposure induced oxidative stress and mitochondrial structural damage by increasing the levels of reactive oxygen species in intestinal tissue. CytoC and Bax transcription, as well as caspase 9 and caspase 3 activities, increased significantly. Significant downregulation of Bcl-2 transcription promoted apoptosis in tadpole intestinal cells. MC-LR exposure disrupted intestinal microbiota and metabolism in tadpoles. Correlation analysis revealed a strong association between intestinal microbiota and oxidative stress, inflammation, immunity, and tissue damage in the intestine. Conclusively, this study provides the first demonstration that MC-LR significantly affects amphibian intestinal microbiota, highlighting tadpoles’ susceptibility to environmental risks posed by MC-LR.

关于微胱氨酸-亮氨酸精氨酸（MC-LR）对两栖动物肠道健康的影响，特别是MC-LR在暴露于环境浓度后如何干扰肠道微生物群，仍存在不确定性。在这项研究中，在30天的时间里，Lithobates catesbeianus蝌蚪暴露于不同浓度的MC-LR（0、0.5和2微克/升）中。目的是研究暴露于MC-LR后，蝌蚪肠道微生物群与肠道屏障之间相互作用的改变如何影响肠道健康。在低浓度环境暴露于MC-LR后，蝌蚪肠道组织受损。它的渗透性增加，病原体抑制能力降低，消化功能受损。此外，肠道组织内脂多糖含量显著增加，下游应答基因TLR4、MyD88、NF-κB表达上调。因此，嗜酸性粒细胞计数和促炎细胞因子表达增加。此外，MC-LR暴露通过增加肠道组织中活性氧的水平诱导氧化应激和线粒体结构损伤。细胞c和Bax转录以及caspase 9和caspase 3活性显著升高。Bcl-2转录显著下调促进蝌蚪肠细胞凋亡。MC-LR暴露破坏了蝌蚪的肠道微生物群和代谢。相关分析显示，肠道微生物群与肠道氧化应激、炎症、免疫和组织损伤之间存在很强的相关性。总之，本研究首次证明了MC-LR对两栖动物肠道微生物群的显著影响，突出了蝌蚪对MC-LR带来的环境风险的易感性。

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引用次数: 0

Bioaccumulation and toxicokinetics of arsenite (As III) in the aquatic bioindicator Pomacea canaliculata using radiotracer analysis 用放射性示踪剂分析水生生物指示剂Pomacea canaliculata中亚砷酸盐（As III）的生物积累和毒性动力学

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107243

Andrea Juarez , Alejandra D. Campoy-Diaz , Luis S. Mayorga , María A. Arribére , Israel A. Vega

This study examines the kinetics of absorption, distribution and accumulation of arsenite (As III) in the freshwater gastropod Pomacea canaliculata using a short-lived tracer (⁷⁶As III). The toxicokinetic model indicate that the gills play a crucial role in the As III uptake, with uptake rates significantly exceeding those of release back into the aquatic environment. The movement of As III from the gills to the hemolymph has low exchange rate. The hemolymph acts as a convective transport medium, facilitating the distribution of As III to various organs of accumulation and elimination within the snail. The majority of As III detected in the hemolymph is directed toward the kidney, stomach, coiled gut and digestive gland. Following feeding, the maximum As inventory (90 %) was reached at 24 h, after which an elimination phase with approximately 4 % between 24 and 96 h According to our toxicokinetic model, feces is the primary route of elimination would be. By 96 h, As III is localized in the digestive gland (70 %) and visceral mass (6 %). Furthermore, tissue accumulation shows a linear correlation with increasing As III concentrations in the surrounding water. By integrating ⁷⁶As radiotracer assays with physiological information, we gain valuable insights in the toxicokinetic of both As III and As V. We hypothesize that biotransformation may occur between these two inorganics As forms at the tissue level.

本研究利用短寿命示踪剂（76As III）研究了淡水腹足动物Pomacea canaliculata对亚砷酸盐（As III）的吸收、分布和积累动力学。毒物动力学模型表明，鳃在亚砷酸盐（As III）的吸收中起着至关重要的作用，其吸收速率显著超过释放回水生环境的速率。As - III从鳃到血淋巴的运动具有低交换率。血淋巴作为一种对流运输介质，促进了砷在蜗牛体内分布到各个器官的积累和消除。血淋巴中检测到的As III主要流向肾脏、胃、盘绕肠和消化腺。饲喂后，24 h时砷含量达到最大值（90%），24 ~ 96 h为排毒期，排毒率约为4%。根据我们的毒物动力学模型，粪便是排毒的主要途径。到96小时，As III局限于消化腺（70%）和内脏肿块（6%）。此外，组织积累与周围水体中砷浓度的增加呈线性相关。通过将76As放射性示踪分析与生理信息相结合，我们获得了对As III和As v的毒性动力学的有价值的见解。我们假设这两种无机物As形式在组织水平上可能发生生物转化。

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引用次数: 0

The impact of bisphenol A on gill health: A focus on mitochondrial dysfunction induced disorders of energy metabolism and apoptosis in Meretrix petechialis

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107259

Xiaotian Wang , Shangjie Zhou , Jianhao Dong , Zhengjia Wei , Yan Liu , Yutong Huang , Junhui Sui , Long Zhu

Bisphenol A (BPA), a well-known chemical compound used in various daily goods, has been associated with adverse effects on animal metabolic processes. However, the specific impacts of BPA exposure on clam gills remain largely unexplored. To investigate the effects of BPA on energy metabolism and apoptosis in Meretrix petechialis gills, clams were exposed to varying concentrations of BPA (1, 10, and 100 μg/L) for 21 days. Results showed that BPA exposure induced gill histopathological injuries and inhibited filtration rates. Transmission electron microscopy (TEM) analysis revealed mitochondrial injury and dysfunction as potential mechanisms of gill damage. Transcriptome analysis identified differentially expressed genes (DEGs) primarily enriched in energy metabolism and apoptosis pathways. BPA-induced changes in ATP content, ATPase, and lactate dehydrogenase (LDH) activities suggested dysregulation of energy metabolism. TUNEL staining demonstrated enhanced apoptotic signals with increasing BPA concentrations. Activation of the caspase-3/9 pathway indicated a concentration-dependent, mitochondria-dependent apoptotic process. Additionally, the expression of genes associated with mitochondria (NNT, TOMM40, and SLC25A11), energy metabolism (PCK1 and pdhC), inducing mitochondria-dependent apoptosis (NFKB1, RAC1, and TRAF2), and oxidative stress (GSTT1) was affected by BPA exposure. Integrated biomarker response version 2 (IBRv2) values further confirmed a concentration-dependent gill toxicity of BPA via the mitochondrial pathway. These findings provide a deeper understanding of the toxicological mechanisms underlying BPA-induced toxicity in bivalves and contribute to assessing the risks posed by BPA in benthic ecosystems.

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引用次数: 0

Mitigating oxidative stress in oyster larvae: Curcumin promotes enhanced redox balance, antioxidant capacity, development, and resistance to antifouling compounds 减轻牡蛎幼虫的氧化应激：姜黄素促进氧化还原平衡、抗氧化能力、发育和对防污化合物的抗性

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107231

Heloísa Bárbara Gabe , Fernando Ramos Queiroga , Karine Amabile Taruhn , Rafael Trevisan

Curcumin (CUR) is a natural compound recognized for stimulating the expression of antioxidant genes. This characteristic has been used to promote animal health and production in aquaculture settings. We hypothesized that supplementing embryos of Crassostrea gigas oysters with CUR would improve their antioxidant capacity, development, and resilience to stress. Embryos were exposed to CUR ranging from 0.03 to 30 µM for 24 h. Their development was assessed, along with measurements of glutathione levels, glutathione S-transferase activity, antioxidant capacity, production of reactive oxygen species (ROS), metabolic activity, and resistance to organic hydroperoxide and the antifouling compound dichlorooctylisothiazolinone (DCOIT). Low curcumin concentrations (up to 1 μM) activated the d-larvae antioxidant system, with a significant threefold increase in glutathione levels and a 50 % decrease in ROS production. This enhancement in antioxidant defense improved the ability of larvae to detoxify organic hydroperoxide. It also resulted in larger larval size and increased survival rates, whether under normal conditions or exposure to peroxide or DCOIT. CUR shows great promise in supporting larval development, but high concentrations were toxic (EC₅₀ = 2.90 μM), probably due to excessive antioxidant activation. Our results indicate that the antioxidant system may play a role in controlling bivalve early development. Understanding how antioxidants influence redox balance and gene expression during early life can enhance our knowledge of stress response mechanisms in marine organisms, offering insights into how they cope with pollutants and environmental challenges. Integrating CUR and antioxidant defense pathway approaches into aquaculture practices could boost productivity and sustainability in oyster aquaculture.

姜黄素（Curcumin， CUR）是一种刺激抗氧化基因表达的天然化合物。这一特点已被用于促进水产养殖环境中的动物健康和生产。我们假设在长牡蛎胚胎中添加CUR可以提高它们的抗氧化能力、发育和抗应激能力。胚胎暴露在0.03至30µM的低温环境中24小时。评估胚胎的发育情况，同时测量谷胱甘肽水平、谷胱甘肽s -转移酶活性、抗氧化能力、活性氧（ROS）的产生、代谢活性以及对有机过氧化氢和抗污染化合物二氯基基异噻唑啉酮（DCOIT）的抗性。低姜黄素浓度（高达1 μM）激活了d-幼虫的抗氧化系统，使谷胱甘肽水平显著增加三倍，ROS生成减少50%。这种抗氧化防御的增强提高了幼虫对有机过氧化氢解毒的能力。它还导致更大的幼虫尺寸和提高存活率，无论是在正常条件下或暴露于过氧化氢或DCOIT。CUR在支持幼虫发育方面有很大的前景，但高浓度可能是有毒的（EC50 = 2.90 μM），可能是由于过度的抗氧化激活。我们的结果表明，抗氧化系统可能在控制双壳类动物的早期发育中起作用。了解抗氧化剂如何影响生命早期的氧化还原平衡和基因表达，可以增强我们对海洋生物应激反应机制的认识，为它们如何应对污染物和环境挑战提供见解。将CUR和抗氧化防御途径纳入养殖实践可以提高牡蛎养殖的生产力和可持续性。

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引用次数: 0

Phenanthrene toxicity during early development of the neotropical tree frog Dendropsophus branneri 新热带树蛙发育早期的菲毒性

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107239

Natallia Vívian Nascimento da Silva Maia , Maria Karolaine de Melo Alves , Célio Freire Mariz Jr , Pedro Ivo Simões , Paulo Sérgio Martins de Carvalho

Phenanthrene is considered a priority polycyclic aromatic hydrocarbon due to its ubiquitous presence in aquatic and terrestrial environments and its toxic potential. Tadpoles are sensitive ecotoxicological models that provide important information regarding effects of contaminants in amphibian species. The goal of the present study was to generate information regarding the acute and chronic toxicity of phenanthrene to the neotropical tree frog Dendropsophus branneri early life stages. Tadpoles at Gosner stage 25 were exposed to environmentally relevant concentrations of 10.8, 18.6, 76.2, 187.7, and 279.6 µg phenanthrene L⁻¹ in two experiments with 15 and 60 days of exposure. Lethality, progression of development, final weight, swimming behavior and enzymatic biomarkers were analyzed. The lethal concentration of dissolved phenanthrene for 50 % of D. branneri tadpoles after 15 days of exposure was 154.8 µg L⁻¹, typical of a sensitive species. Significant developmental delay was verified, with a lowest observed effect concentration (LOEC) of 76.2 µg L⁻¹ and 10.8 µg L⁻¹ after exposures of 15 and 60 days, respectively. Delay was measured based on the difference between the mean Gosner stage of controls and exposed tadpoles (Delta_gosner). Delta_gosner after 60 days of exposure ranged from 10 at 10.8 µg L⁻¹ to 13 at 187.7 µg L⁻¹, and tadpoles remained in the early stages of hind limb development (Gosner stages 28–32), in contrast to controls that reached stage 42 with fully formed forelimbs and hind limbs with toes at metamorphic climax. Glutathione-S-transferase and Catalase were induced in exposed tadpoles, possibly due to oxidative stress. Thigmotaxis was decreased in phenanthrene exposed tadpoles, indicating loss of anti-predatory behaviors. Final wet weight of exposed D.branneri tadpoles significantly decreased. Delayed development and slower growth rate are crucial factors for tadpole survival, and these effects of phenanthrene on D.branneri early stages potentially affects its recruitment to the adult stage.

菲被认为是优先考虑的多环芳烃，因为它普遍存在于水生和陆地环境中，并且具有潜在的毒性。蝌蚪是敏感的生态毒理学模型，为污染物对两栖动物的影响提供了重要的信息。本研究的目的是产生有关菲对新热带树蛙早期急性和慢性毒性的信息。在两个实验中，Gosner阶段25的蝌蚪分别暴露于10.8、18.6、76.2、187.7和279.6µg的环境相关浓度的菲L−1，暴露时间分别为15和60天。分析致死率、发育进程、最终体重、游泳行为和酶生物标志物。暴露15天后，50%的布氏蝌蚪溶解菲的致死浓度为154.8µg L−1，是典型的敏感物种。研究证实了显著的发育延迟，暴露15天和60天后的最低效应浓度（LOEC）分别为76.2µg L - 1和10.8µg L - 1。延迟是根据对照组和暴露的蝌蚪（Deltagosner）平均Gosner阶段之间的差异来测量的。暴露60天后的Deltagosner浓度从10.8µg L - 1的10到187.7µg L - 1的13不等，并且蝌蚪仍处于后肢发育的早期阶段（Gosner阶段28-32），而对照组的前肢和后肢发育完全，处于变形高潮。暴露的蝌蚪体内的谷胱甘肽- s -转移酶和过氧化氢酶被诱导，可能是由于氧化应激。暴露于菲的蝌蚪趋近性降低，表明其丧失了反捕食行为。暴露后的布氏小蝌蚪最终湿重显著降低。发育迟缓和生长速度减慢是影响蝌蚪存活的关键因素，而菲对早期布氏小蝌蚪的影响可能会影响其在成虫期的招募。

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引用次数: 0

Gender-specific effects of dydrogesterone on zebrafish liver metabolism after long-term exposure 长期接触地屈孕酮对斑马鱼肝脏代谢的性别特异性影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107236

Yu-Xia Jiang , Jian-Nan Guo , Li-Xin Hu , Hui Zhang , Choon-Nam Ong , Wen-Jun Shi , Guang-Guo Ying

Synthetic progestin dydrogesterone is widely used in gynecology and animal husbandry, leading to high environmental detection rates and concentrations. Dydrogesterone influences sex differentiation, gonad development, and spawning in fish. However, its impact on the liver, a vital organ for hormone production and detoxification, remains largely unknown. In this study, zebrafish embryos were exposed to 2.8, 27.6, and 289.8 ng/L of dydrogesterone until they reached sexual maturity. Metabolomics and Fourier transform infrared spectroscopy (FTIR) were employed to investigate alterations in the zebrafish liver. Long-term exposure to dydrogesterone decreased body weight and length in females but increased them in males. The levels of phospholipids, monoglycerides, lysophospholipids, fatty acids, acylcarnitines, acyltaurines, cholesterol, and bile acids increased in the liver of females but decreased in males due to dydrogesterone, making the metabolic pathways the most affected. FTIR analysis revealed a reduction in lipid and protein absorption coupled with an increase in carbohydrate absorption in the liver of exposed males, whereas exposed females exhibited reductions in both lipid and carbohydrate absorption. These findings suggest that long-term exposure to dydrogesterone enhances basic metabolism and physical growth in male zebrafish. To the best of our knowledge, this is the first report on the effects of progestins on body metabolism. Additionally, we find that gender difference is a notable feature of the effects of dydrogesterone on zebrafish.

合成黄体酮地屈孕酮广泛应用于妇科和畜牧业，环境检出率和浓度较高。地屈孕酮影响鱼类的性别分化、性腺发育和产卵。然而，它对肝脏的影响，一个重要的器官激素生产和解毒，仍然是未知的。在这项研究中，斑马鱼胚胎分别暴露于2.8、27.6和289.8 ng/L的地孕酮中，直到它们达到性成熟。利用代谢组学和傅里叶变换红外光谱（FTIR）研究斑马鱼肝脏的变化。长期接触地屈孕酮会使雌性体重和体长减少，而雄性体重和体长则增加。磷脂、单甘油三酯、溶血磷脂、脂肪酸、酰基肉碱、酰基牛磺酸、胆固醇和胆汁酸的水平在女性肝脏中升高，而在男性肝脏中由于地屈孕酮的作用而降低，代谢途径受到的影响最大。FTIR分析显示，暴露的男性肝脏中脂质和蛋白质吸收减少，同时碳水化合物吸收增加，而暴露的女性肝脏中脂质和碳水化合物吸收减少。这些发现表明，长期接触地屈孕酮可以促进雄性斑马鱼的基本代谢和身体生长。据我们所知，这是关于黄体酮对身体代谢影响的第一份报告。此外，我们发现性别差异是地屈孕酮对斑马鱼的影响的显著特征。

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引用次数: 0