Aquatic Toxicology最新文献_第2页

Combined effects of polymethylmethacrylate microplastics with arsenic and copper on the euryhaline rotifer Proales similis 聚甲基丙烯酸甲酯微塑料与砷和铜对广盐轮虫的联合作用。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107214

Uriel Arreguin-Rebolledo , Federico Páez-Osuna , Gladys Valencia-Castañeda , Mario Alberto Arzate-Cárdenas , Mariana V. Capparelli

Aquatic organisms are typically exposed to chemical mixtures, including microplastics and metal(loid)s. However, most research has primarily focused on the toxicity of individual chemicals, potentially overestimating their risks to aquatic life. This study examined the combined effects of polymethylmethacrylate microplastics (PMMA-MPs) with As and Cu at environmentally relevant concentrations on the euryhaline rotifer Proales similis. The 48-h EC₅₀ of PMMA-MPs (1.5–11.5 μm) was determined using concentrations from 1.0 to 100 mg/L. These concentrations were then combined with 0.41 mg/L As or 0.034 mg/L Cu. The combined effects of PMMA-MPs (0.031–1.0 mg/L) with As (0.025–0.2 mg/L) and Cu (0.0025–0.02 mg/L) at environmental concentrations were assessed through five-day chronic toxicity reproductive tests. The population growth rate was the endpoint for both acute and chronic testing. The EC₅₀ of PMMA-MPs for P. similis was 44.0 mg/L, but this value significantly decreased when PMMA-MPs were co-exposed with the tested metal(loid)s. The tested mixtures of PMMA-MPs with As and Cu reduced growth rates by 13 – 48 % and 11 – 35 %, respectively, compared to the individual exposure to each chemical. This indicates that PMMA-MPs alter the toxic behavior of these metal(loid)s and vice versa. The present findings reveal that the combination of environmentally relevant concentrations of MPs (modeled as PMMA-MPs) with As and Cu can induce synergistic effects in marine rotifers. These results highlight the importance of investigating pollution in realistic scenarios that many aquatic invertebrates face.

水生生物通常暴露于化学混合物中，包括微塑料和金属（胶体）。然而，大多数研究主要集中在个别化学品的毒性上，可能高估了它们对水生生物的风险。本研究考察了聚甲基丙烯酸甲酯微塑料（PMMA-MPs）与环境相关浓度的砷和铜对泛盐轮虫的联合作用。采用1.0 ~ 100 mg/L浓度测定PMMA-MPs (1.5 ~ 11.5 μm) 48h EC50。然后将这些浓度与0.41 mg/L的砷或0.034 mg/L的铜混合。通过5天慢性毒性生殖试验，评估环境浓度下PMMA-MPs （0.031-1.0 mg/L）与砷（0.025-0.2 mg/L）和铜（0.0025-0.02 mg/L）的联合效应。人口增长率是急性和慢性试验的终点。PMMA-MPs的EC50值为44.0 mg/L，但当PMMA-MPs与所测金属（样物质）共暴露时，EC50值显著降低。测试的PMMA-MPs与As和Cu的混合物与单独暴露于每种化学物质相比，分别降低了13 - 48%和11 - 35%的生长速率。这表明PMMA-MPs改变了这些金属（类）s的毒性行为，反之亦然。本研究结果表明，环境相关浓度的MPs（建模为PMMA-MPs）与as和Cu的结合可以诱导海洋轮虫的协同效应。这些结果强调了在许多水生无脊椎动物面临的现实情况下调查污染的重要性。

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引用次数: 0

Histopathological and histochemical analysis of the digestive tract of adult horseshoe crabs under mercury stress 汞胁迫下成年鲎消化道的组织病理学和组织化学分析

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107202

Wanling Zhang , Mujiao Xie , Xiaohai Chen , Ziwei Ying , Yuyuan Bao , Yang-Guang Gu , Xiaoyong Xie

Mercury (Hg) contamination is a major environmental concern that continuously impacts marine organisms, including the mangrove horseshoe crab (Carcinoscorpius rotundicauda). As a vulnerable species, C. rotundicauda holds significant ecological and evolutionary value, making it particularly susceptible to Hg pollution and deserving of focused conservation efforts. This study presents the first comprehensive histopathological and histochemical analysis of mercury toxicity in the digestive tract of adult C. rotundicauda. Through both qualitative and quantitative analyses of histopathological and histochemical alterations, we evaluated the effects of acute Hg exposure (0.5 mg/L and 5 mg/L) on the esophagus, stomach, and intestines over time. The results revealed significant dose- and time-dependent tissue damage, with the stomach exhibiting the most pronounced alterations, including epithelial detachment, vacuolation caused by fibers degradation in the loose connective tissue, and muscle layer disruption, followed by the esophagus and intestines. Additionally, mucous cell density in the hindgut submucosa decreased by 30 % after 96 h of acute Hg exposure (0.5 and 5 mg/L HgCl₂), indicating a weakened protective barrier. The tissue damage of the digestive tract may further have a negative impact on the health of the adult horseshoe crab, thus threatening the survival of C. rotundicauda population and reducing the biodiversity of the mangrove ecosystem. These findings underscore the critical threat posed by Hg pollution to the digestive system of C. rotundicauda and offer novel insights into the toxicological impact on marine invertebrates. Considering the tissue-damaging effects of Hg on adult horseshoe crabs, this study underscores the importance of regular monitoring of Hg contamination in natural habitats. The results offer valuable guidance for future habitat restoration and effective management of existing habitats.

汞污染是一个主要的环境问题，不断影响海洋生物，包括红树林马蹄蟹（Carcinoscorpius rotundicauda）。作为一种易危物种，圆尾螺具有重要的生态和进化价值，对汞污染尤为敏感，值得重点保护。本研究提出的第一个全面的组织病理学和组织化学分析汞毒性在消化道的成年rotundicauda。通过组织病理学和组织化学变化的定性和定量分析，我们评估了急性汞暴露（0.5 mg/L和5 mg/L）对食管、胃和肠道的影响。结果显示了明显的剂量和时间依赖性组织损伤，胃表现出最明显的改变，包括上皮脱离，疏松结缔组织纤维降解引起的空泡化，肌肉层破坏，其次是食道和肠道。此外，在急性汞暴露（0.5和5 mg/L HgCl2） 96小时后，后肠粘膜下层的粘膜细胞密度下降了30%，表明保护屏障减弱。消化道的组织损伤可能进一步对成年马蹄蟹的健康产生负面影响，从而威胁到圆尾蟹种群的生存，降低红树林生态系统的生物多样性。这些发现强调了汞污染对圆尾螺消化系统的严重威胁，并为海洋无脊椎动物的毒理学影响提供了新的见解。考虑到汞对成年马蹄蟹的组织损伤作用，本研究强调了在自然栖息地定期监测汞污染的重要性。研究结果为今后生境恢复和现有生境的有效管理提供了有价值的指导。

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引用次数: 0

Polystyrene microplastics exacerbate acetochlor-induced reproductive toxicity and transgenerational effects in zebrafish 聚苯乙烯微塑料加剧乙氯诱导的斑马鱼生殖毒性和跨代效应

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107208

Yang Wang , Chaofan Ma , Zenglong Chen , Yinping Guo , Yuan Jing , Xiaolei Chen , Hongwu Liang

Microplastic (MPs) can adsorb co-existing pollutants, and alter their behavior and toxicity. Meanwhile, amide herbicides like acetochlor (ACT) are widely used in agriculture, with potential endocrine-disrupting effects that raise ecological concerns. The aim of this research was to examine the effects of MPs on the reproductive endocrine disruption caused by ACT and the effects of maternal transmission. Zebrafish were employed in this study to assess the reproductive toxicity of ACT alone and in combination with polystyrene microplastics (PS-MPs) of different sizes (200 nm and 2 μm) and concentrations (0.1 and 1 mg/L) over a 63-day exposure experiment. The results indicated that ACT was concentrated in zebrafish tissues in the order: intestine > liver > gill > brain > gonad > muscle. PS-MPs increased ACT bioaccumulation, worsened gonadal damage, led to abnormalities in hormone levels, and caused disruptions in HPG axis gene expression, further exacerbating the reproductive toxicity. Maternal transfer of ACT affected offspring growth, thyroid function, and HPT axis gene expression, with nanoplastics (NPS) amplifying these adverse effects. This study offers crucial insights into the ecological hazards posed by ACT and PS-MPs, emphasizing the increased toxicity due to PS-MPs.

微塑料（MPs）可以吸附共存的污染物，并改变它们的行为和毒性。与此同时，乙草胺（ACT）等酰胺类除草剂被广泛用于农业，其潜在的内分泌干扰效应引起了生态问题。本研究的目的是检查MPs对ACT引起的生殖内分泌紊乱的影响以及母体传播的影响。本研究利用斑马鱼进行了为期63天的暴露实验，以评估ACT单独暴露和不同尺寸（200 nm和2 μm）、不同浓度（0.1和1 mg/L）的聚苯乙烯微塑料（PS-MPs）的生殖毒性。结果表明，ACT在斑马鱼组织中的富集顺序为：肠；肝脏在吉尔比;大脑比;性腺祝辞肌肉。PS-MPs增加ACT生物蓄积，加重性腺损伤，导致激素水平异常，破坏HPG轴基因表达，进一步加剧生殖毒性。母体转移ACT影响后代生长、甲状腺功能和HPT轴基因表达，而纳米塑料（NPS）放大了这些不利影响。这项研究对ACT和PS-MPs的生态危害提供了重要的见解，强调了PS-MPs引起的毒性增加。

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引用次数: 0

Cetacean species found stranded along Croatian coast of the Adriatic Sea as bioindicators of non-essential trace elements in the environment 在克罗地亚亚得里亚海沿岸发现搁浅的鲸类动物，作为环境中非必需微量元素的生物指标

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107206

Marija Sedak , Maja Đokić , Nina Bilandžić , Tomislav Gomerčić , Miroslav Benić , Manuela Zadravec , Martina Đuras

In tissues of toothed whales from the Adriatic Sea (muscle, liver, kidney, lung, spleen, adipose tissue and skin) the concentrations of cadmium (Cd), lead (Pb) and arsenic (As) were analysed. In total, 186 dolphins were analysed; 155 bottlenose (Tursiops truncatus), 25 striped (Stenella coeruleoalba) and 6 Risso's dolphins (Grampus griseus). Cadmium concentrations in tissue samples ranged from 0.001 mg/kg in muscle to 16.8 mg/kg wet weight in kidney. Arsenic concentrations in dolphin samples ranged from 0.010 to 12.9 mg/kg ww. The lowest As concentration was found in spleen and highest in liver of bottlenose dolphin. Cadmium and As levels in Risso's dolphins showed higher concentrations in all tissues in comparison to bottlenose and striped dolphins. >50 % of the measured Pb values for all three species of dolphins and examined tissues were lower than 0.1 mg/kg. The accumulation of Cd and As during the lifetime was confirmed. None of the dolphins analysed in this study were exposed to concentrations of Cd in the liver higher than 20 mg/kg wet weight, which can cause renal failure in marine mammals. Numerous species of marine mammals inhabit coastal environments alongside humans and utilize similar food sources, such as fish and cephalopods. Consequently, these mammals can function as valuable indicators of public health concerns.

分析了亚得里亚海齿鲸组织（肌肉、肝脏、肾脏、肺、脾脏、脂肪组织和皮肤）中镉（Cd）、铅（Pb）和砷（As）的浓度。总共分析了186只海豚；155只宽吻海豚（Tursiops truncatus）， 25只条纹海豚（Stenella coeruleoalba）和6只里索海豚（grpus griseus）。组织样品中的镉浓度范围从肌肉中的0.001 mg/kg到肾脏中的16.8 mg/kg湿重。海豚样本中的砷浓度为0.010至12.9 mg/kg ww。宽吻海豚的砷含量以脾脏最低，肝脏最高。与宽吻海豚和条纹海豚相比，里索海豚体内的镉和砷含量在所有组织中的浓度都更高。所有三种海豚和被检查组织中50%的铅测量值低于0.1 mg/kg。证实了镉和砷在其一生中的积累。在这项研究中分析的海豚中，没有一只暴露在肝脏中镉浓度高于20毫克/公斤湿重的环境中，这可能导致海洋哺乳动物肾功能衰竭。许多种类的海洋哺乳动物与人类一起生活在沿海环境中，并利用类似的食物来源，如鱼类和头足类动物。因此，这些哺乳动物可以作为公共卫生问题的有价值的指标。

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引用次数: 0

Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii) 饲粮中添加绿原酸对豚鼠肠道脂多糖诱导的氧化应激、炎症和细胞凋亡具有保护作用。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107223

Yintao Wang , Sitong Meng , Denglai Li , Siying Liu , Liang LI , Lifang Wu

In this study, the alleviative effects of chlorogenic acid (CGA) on oxidative stress, inflammation and apoptosis of amur ide (Leuciscus waleckii) induced by lipopolysaccharide (LPS) were evaluated. Using a 2 × 2 factorial design, amur ide were irregularity divided into 4 groups and fed two diets with 0.00 % (CK and LPS), 0.04 % CGA(CGA and LC). After 4-week feeding trial, LPS challenge was executed. Results showed that 0.04 % CGA alleviated LPS-induced intestinal barrier dysfunction by decreasing the levels of 5-HT, D-LA, ET-1 and DAO in serum, increasing ZO-1, Occludin-α, Claudin-c, Claudin-f mRNA, and ZO-1, Occludin, Claudin-1 protein expression, improving intestinal morphology. Moreover, 0.04 % CGA alleviated LPS-induced inflammation and apoptosis by up-regulating TGF-β and Bcl-2 mRNA, down-regulating NF-κBp65, TNF-α, Bax, Caspase-3, Caspase-9 mRNA and NF-κBp65, Bax, Caspase-3 protein expression. 0.04 %CGA reversed LPS-induced the reduction of GSH-PX, CAT, T-SOD and T-AOC in intestines, whereas MDA showed the opposite result. 0.04 % alleviated LPS-induced the decrease of Nrf2, HO-1, CAT, SOD mRNA and Nrf2 protein expression, the increase of Keap1 mRNA. Summary, this study suggested that 0.04 % of dietary CGA alleviated LPS-induced intestinal oxidative stress, inflammation and apoptosis of amur ide.

本研究探讨了绿原酸（CGA）对脂多糖（LPS）诱导的白桦（Leuciscus waleckii）氧化应激、炎症和凋亡的缓解作用。采用2 × 2因子设计，随机分为4组，分别饲喂0.00% （CK + LPS）和0.04% CGA（CGA + LC）的饲粮。饲喂4周后，进行LPS攻毒。结果表明，0.04% CGA可通过降低血清5-HT、D-LA、ET-1和DAO水平，提高ZO-1、Occludin-α、Claudin-c、Claudin-f mRNA和ZO-1、Occludin、Claudin-1蛋白表达，改善肠道形态，减轻lps诱导的肠道屏障功能障碍。0.04% CGA通过上调TGF-β和Bcl-2 mRNA，下调NF-κBp65、TNF-α、Bax、Caspase-3、Caspase-9 mRNA和NF-κBp65、Bax、Caspase-3蛋白表达，减轻lps诱导的炎症和凋亡。0.04% CGA能逆转lps诱导的肠内GSH-PX、CAT、T-SOD和T-AOC的降低，而MDA则相反。0.04%可减轻lps诱导的Nrf2、HO-1、CAT、SOD mRNA和Nrf2蛋白表达的降低，Keap1 mRNA表达的升高。综上所述，本研究提示，饲粮中添加0.04%的CGA可减轻lps诱导的小鼠肠道氧化应激、炎症和凋亡。

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引用次数: 0

Response of the photosynthetic physiology of Ulva lactuca to Cu toxicity under ocean acidification 海洋酸化条件下海藻光合生理对Cu毒性的响应。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107222

Tianpeng Xu , Zhouyue Lu , Cheng Chen , Yuxin Xie , Jing Ma , Juntian Xu

Ocean acidification can significantly affect the physiological performance of macroalgae. While copper (Cu) is an essential element for macroalgae and has been extensively studied, the interactive effects of ocean acidification and Cu on these organisms remain less understood. In this study, we measured the photosynthetic characteristics of Ulva lactuca exposed to varying Cu concentrations at two CO₂ levels (415 ppmv, low concentration; 1000 ppmv, high concentration). The results indicated that during chronic toxicity testing, the growth of juvenile U. lactuca significantly increased at Cu concentrations of 0.001 μM, 0.01 μM, and 0.1 μM regardless of low CO₂ concentrations or high CO₂ concentrations condition. In acute toxicity tests, elevated Cu concentrations negatively impacted the growth rate, yield, and photosynthetic rate of U. lactuca under low CO₂ concentrations. Conversely, high CO₂ concentrations enhanced the photosynthetic capacity of U. lactuca with increased Cu concentrations, while the growth rate significantly decreased at Cu concentration of 1.5 μM. Additionally, the activities of peroxidase (POD) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) increased, with an enhancement of malondialdehyde (MDA) content at 1.5 μM Cu under high CO₂ conditions. However, the structure of the chloroplast thylakoid was disrupted by elevated Cu concentrations. These findings suggest that low Cu concentrations promote the growth of U. lactuca, whereas high Cu concentrations inhibit algal growth, and ocean acidification may exacerbate the adverse effects of Cu on U. lactuca in acute toxicity tests.

海洋酸化会显著影响大型藻类的生理性能。虽然铜（Cu）是大型藻类的必需元素，并已被广泛研究，但海洋酸化和Cu对这些生物的相互作用仍然知之甚少。在本研究中，我们测量了在两个CO2水平(415 ppmv，低浓度；1000 ppmv，高浓度)。结果表明：在慢性毒性试验中，无论低CO2浓度还是高CO2浓度，铜浓度分别为0.001 μM、0.01 μM和0.1 μM时，乳藻幼鱼的生长均显著增加；在急性毒性试验中，在低CO2浓度下，Cu浓度升高对U. lactuca的生长速率、产量和光合速率有负面影响。相反，随着Cu浓度的增加，高CO2浓度可以增强U. lactuca的光合能力，而在Cu浓度为1.5 μM时，生长速率显著降低。此外，在1.5 μM Cu浓度下，随着丙二醛（MDA）含量的增加，过氧化物酶（POD）和核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）活性增加。然而，叶绿体类囊体的结构被Cu浓度升高所破坏。这些结果表明，低Cu浓度可促进乳藻的生长，而高Cu浓度可抑制藻类的生长，并且在急性毒性试验中，海洋酸化可能加剧Cu对乳藻的不良影响。

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引用次数: 0

Combined effects of a pharmaceutical pollutant, gemfibrozil, and abiotic stressors (warming and air exposure) on cellular stress responses of the blue mussels Mytilus edulis 药物污染物、吉非纤维唑和非生物应激源（升温和空气暴露）对蓝贻贝细胞应激反应的联合影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107233

Halina Falfushynska , Eugene P. Sokolov , Inna M. Sokolova

Lipid-lowering drugs such as gemfibrozil (GFB) are widely used and highly biologically active, contributing to their persistence in wastewater and subsequent release into aquatic ecosystems. However, the potential impacts and toxic mechanisms of these emerging pollutants on non-target marine organisms, particularly keystone bivalves like Mytilus edulis, remain poorly understood. To address this knowledge gap, we investigated the effects of environmentally relevant concentrations of GFB (25 µg l⁻¹) on oxidative, nitrosative, and dicarbonyl stress in M. edulis, and explored how abiotic stressors such as elevated temperature and air exposure modulate these effects. Our results indicated that GFB and temperature interact to significantly influence oxidative stress markers, including lipid peroxidation (LPO) and protein carbonylation (PC) levels in mussels. Notably, the combination of GFB and warming exhibited antagonistic effects, leading to reduced LPO levels in both submerged and air-exposed mussels. Air exposure alone elevated PC levels across all groups, while warming reduced these levels. Total antioxidant capacity increased during air exposure, with GFB exerting minimal influence on this parameter. Nitrosative stress, as indicated by nitric oxide levels, was significantly affected by GFB only under air exposure conditions. The glutathione system underwent notable alterations, with glutathione reductase activity stimulated during immersion and suppressed during air exposure. Dicarbonyl stress markers, including methylglyoxal and glyoxalase enzyme activities, generally intensified in response to GFB during air exposure. Overall, environmentally relevant concentrations of GFB induced oxidative and dicarbonyl stress in M. edulis, suggesting a shift toward glycolytic metabolism that could impair energy-dependent processes like reproduction. Combined stressor scenarios involving GFB and warming typically exhibited antagonistic rather than synergistic effects. Despite these biochemical disruptions, the mussels demonstrated resilience, particularly during air exposure, highlighting the complexity of environmental stress interactions. These findings emphasize the importance of considering multiple stressors in pollution risk assessments for aquatic ecosystems.

吉非罗齐（gemfibrozil， GFB）等降脂药物被广泛使用并具有很高的生物活性，这有助于它们在废水中持续存在并随后释放到水生生态系统中。然而，这些新出现的污染物对非目标海洋生物的潜在影响和毒性机制，特别是像Mytilus edulis这样的关键双壳类，仍然知之甚少。为了解决这一知识差距，我们研究了环境相关浓度的GFB （25 μ g l−1）对毛毛杆菌氧化、亚硝化和二羰基应激的影响，并探讨了温度升高和空气暴露等非生物应激源如何调节这些影响。我们的研究结果表明，GFB和温度相互作用显著影响贻贝氧化应激标志物，包括脂质过氧化（LPO）和蛋白质羰基化（PC）水平。值得注意的是，GFB和变暖的组合表现出拮抗作用，导致水下和空气暴露贻贝的LPO水平降低。仅暴露在空气中就会提高所有人群的PC水平，而变暖则会降低这些水平。总抗氧化能力在空气暴露期间增加，GFB对该参数的影响最小。一氧化氮水平表明，只有在空气暴露条件下，GFB才会显著影响亚硝化应激。谷胱甘肽系统发生了显著的变化，谷胱甘肽还原酶活性在浸泡期间受到刺激，在空气暴露期间受到抑制。二羰基应激标志物，包括甲基乙二醛和乙二醛酶活性，在空气暴露期间普遍增强。总的来说，环境相关浓度的GFB诱导了毛毛杆菌的氧化和二羰基应激，表明糖酵解代谢的转变可能损害像繁殖这样的能量依赖过程。包括GFB和变暖的联合应激情景通常表现出拮抗而不是协同效应。尽管存在这些生化破坏，但贻贝表现出了恢复能力，特别是在暴露于空气中的情况下，这突出了环境压力相互作用的复杂性。这些发现强调了在水生生态系统污染风险评估中考虑多重压力源的重要性。

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引用次数: 0

Omics insights in responses of bivalves exposed to plastic pollution 双壳类动物对塑料污染反应的组学见解

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107224

Robert Mkuye , Chuangye Yang , Fortunatus Masanja , Salifu Ibrahim , Xiuyan Yang , Happiness Mwemi , Peter Mrope , Muhammed Salman , Alma Alfatat , Yuewen Deng

Plastic pollution, particularly microplastics and nanoplastics, poses a significant threat to marine ecosystems. Bivalves, vital filter feeders that accumulate plastic particles, underscore the necessity for advanced omics technologies to grasp their molecular reactions to plastic exposure.

This review delves into the impact of microplastics and nanoplastics on bivalves utilizing advanced omics technologies. Through an examination of omics data, this review sheds light on how bivalves react to plastic pollution, informing strategies for conservation and food safety. Furthermore, theoretical pathways have been formulated to decipher how bivalves respond to environmental stressors from microplastics or nanoplastics through the integration of diverse biological fields. In this review, we report that microplastics and nanoplastics in marine ecosystems primarily stem from human activities on land and in marine domains. Bivalves are negatively influenced by plastic contamination, impacting their health and economic worth. Exposure to plastic particles disrupts bivalve behavior, metabolism, and reproduction, precipitating health concerns. Integration of omics data is instrumental in unraveling molecular interactions and devising biomarkers for monitoring purposes. Ingestion of plastics by bivalves poses risks to human health. Additionally, mitigation tactics involve bans, levies, and advocating for biodegradable alternatives to curtail plastic pollution. The amalgamation of omics findings aids in the comprehension of bivalve responses and effectively addressing plastic pollution. Moreover, addressing plastic pollution necessitates a multidisciplinary approach encompassing scientific inquiry, regulatory frameworks, and collaboration with stakeholders. These strategies are paramount in safeguarding bivalves, marine ecosystems, food safety, and human health.

塑料污染，特别是微塑料和纳米塑料，对海洋生态系统构成重大威胁。双壳类动物是一种重要的滤食性动物，它们会积聚塑料颗粒，因此需要先进的组学技术来掌握它们对塑料暴露的分子反应。

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引用次数: 0

Long-term exposure to sediment-associated antidepressants impacts life-history traits in an estuarine deposit-feeding worm 长期接触与沉积物相关的抗抑郁药物会影响河口沉积物食性蠕虫的生活史特征。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107189

Martina Santobuono, Wing Sze Chan, Elettra D´Amico, Henriette Selck

Hydrophobic pollutants, such as the antidepressant sertraline (SER), tend to sorb to particles in the water column and subsequently accumulate in the sediment. Long-term exposure to these pollutants may significantly affect sediment-dwelling organisms´ fitness and behavior. To address this knowledge gap, we investigated the impact of chronic exposure to a range of environmentally relevant and higher concentrations of sediment-associated SER on the deposit-feeding polychaete Capitella teleta. Since certain antidepressants can function as neurotoxic chemicals and endocrine disruptors on non-target species, we examined feeding rate and burrowing behavior in adult worms after 23 days of exposure (Experiment 1), and key life-history traits in juvenile worms during 35 days of exposure (Experiment 2) to sediment-associated SER (0.33 - 100 µg/g dw sediment). SER did not affect survival but reduced maturation and time to first reproduction: 37%, 50%, and 29% of the worms exposed respectively to SER 0.33, 3.3 and 33 µg/g reached maturation on day 21, whereas worms in the other treatments did not mature (0%; control) or reached a lower maturation degree (6%; 100 µg/g). Although not statistically significant, growth, feeding, and burrowing manifested non-monotonic trends: at environmentally relevant SER concentrations adults increased feeding and extended time to fully burrow into the sediment, and juveniles increased growth, whereas high concentrations had an inhibitory or no effect. Reproductive endpoints appeared most sensitive to chronic SER exposure. Even at low environmental concentrations, antidepressants can cause sublethal effects in non-target species, potentially affecting population dynamics and ecosystem functioning. Further research is key to fully understanding the ecological impact of hydrophobic chemicals in natural environments.

疏水污染物，如抗抑郁药舍曲林（SER），倾向于吸收水柱中的颗粒，随后积聚在沉积物中。长期暴露于这些污染物可能会显著影响沉积物生物的适应性和行为。为了解决这一知识差距，我们研究了长期暴露于一系列与环境相关的高浓度与沉积物相关的SER对沉积物食性多毛藻的影响。由于某些抗抑郁药可能对非目标物种起神经毒性化学物质和内分泌干扰物的作用，我们在暴露于与沉积物相关的SER （0.33 - 100 μ g/g dw沉积物）23天后检测了成虫的摄食率和挖洞行为（实验1），并在暴露于与沉积物相关的SER （0.33 - 100 μ g/g dw沉积物）35天后（实验2）检测了幼虫的关键生活史特征。SER不影响存活，但减少了成熟和第一次繁殖的时间：分别暴露于SER 0.33、3.3和33µg/g的线虫中，37%、50%和29%的线虫在第21天成熟，而其他处理的线虫没有成熟(0%；对照)或达到较低的成熟程度(6%；100µg / g)。虽然没有统计学意义，但生长、取食和挖洞表现出非单调的趋势：在与环境相关的SER浓度下，成虫增加了取食，延长了充分钻入沉积物的时间，幼虫增加了生长，而高浓度则有抑制作用或没有影响。生殖终点对慢性SER暴露最为敏感。即使在低环境浓度下，抗抑郁药也会对非目标物种造成亚致死效应，可能影响种群动态和生态系统功能。进一步的研究是充分了解疏水化学物质在自然环境中的生态影响的关键。

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引用次数: 0

Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts 滤食性物种的基因作为监测微塑料影响的潜在工具包。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107234

Marta Pilar Ortiz-Moriano, Eva Garcia-Vazquez, Gonzalo Machado-Schiaffino

Microplastics (MPs) are ubiquitous in the marine environment and impact organisms at multiple levels. Understanding their actual effects on wild populations is urgently needed. This study develops a toolkit to monitor changes in gene expression induced by MPs in natural environments, focusing on filter-feeding and bioindicator species from diverse ecological and taxonomic groups. Six candidate genes —Caspase, HSP70, HSP90, PK, SOD, and VTG— and nine filter-feeding species —two branchiopods, one copepod, five bivalves and one fish— were selected based on differential expression in response to MPs exposure (mainly the widely used polystyrene and polyethylene polymers) reported in over 30 publications. Some genes are particularly determinant, such as HSP70 and HSP90 (key to managing a wide range of stressors) and SOD (critical for addressing oxidative stress), as they are more directly related to stress. PK is related to carbohydrate metabolism (alterations in energy metabolism); VTG is associated with reproductive problems; Caspase mediates in apoptosis. Each gene in the toolkit plays a role depending on the type of stress assessed, and their combination provides a comprehensive understanding of the impacts of MPs. Differences in gene expressions between species and the exposure thresholds were found. These genes were examined in various scenarios with different types, concentrations, and sizes of MPs, alone or with other stressors. The toolkit offers significant advantages, allowing a comprehensive study of the impact of MPs and focusing on filtering bioindicator species, thus enabling pollution assessment and long-term monitoring. It will outperform traditional methods like tissue counts of MPs where only physical damage is visible, providing a deeper understanding. To our knowledge, this is the first toolkit of its kind.

微塑料（MPs）在海洋环境中无处不在，并在多个层面影响生物。迫切需要了解它们对野生种群的实际影响。本研究开发了一个工具包来监测自然环境中MPs诱导的基因表达变化，重点关注来自不同生态和分类群体的滤食性和生物指示物种。根据30多篇文献报道的MPs（主要是广泛使用的聚苯乙烯和聚乙烯聚合物）暴露后的差异表达，选择了6个候选基因——caspase、HSP70、HSP90、PK、SOD和VTG，以及9个滤食性物种——2个支足类、1个桡足类、5个双壳类和1个鱼类。一些基因尤其具有决定性，如HSP70和HSP90（管理各种压力源的关键）和SOD（解决氧化应激的关键），因为它们与压力更直接相关。PK与碳水化合物代谢（能量代谢的改变）有关；VTG与生殖问题有关；Caspase介导细胞凋亡。工具包中的每个基因都根据评估的压力类型发挥作用，它们的组合提供了对MPs影响的全面了解。发现不同物种间基因表达和暴露阈值存在差异。这些基因在不同类型、浓度和大小的MPs、单独或与其他压力源的不同情况下进行了检测。该工具包具有显著的优势，允许对MPs的影响进行全面研究，并专注于过滤生物指示物种，从而实现污染评估和长期监测。它将优于传统的方法，如只有物理损伤可见的MPs组织计数，提供更深入的了解。据我们所知，这是同类工具中的第一个。

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引用次数: 0