Aquatic Toxicology最新文献_第10页

Arsenite-induced liver apoptosis via oxidative stress and the MAPK signaling pathway in marine medaka 亚砷酸盐通过氧化应激和MAPK信号通路诱导海洋medaka肝脏凋亡

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107226

Jiangtian Lin , Ting Zhang , Li Zhang

Arsenic (As) is widely recognized for its hazards to aquatic organisms; however, its toxicological impacts on apoptosis in marine fish remain inadequately explored. This study investigated the effects of in vivo dietary exposure to 50 or 500 mg/kg AsIII (as NaAsO₂) over 28 days in marine medaka, alongside in vitro exposure to 50–750 μg/L AsIII for 48 h in a hepatic cell line derived from marine medaka, to elucidate the toxicity and underlying molecular mechanisms. In vivo, As significantly accumulated in liver tissue (1.79-fold compared to the control), causing hepatic lesions and increased apoptosis (4.85 ± 0.56 % and 9.29 ± 1.82 %, respectively). Gene expression analysis showed downregulation of bcl2l1 and upregulation of bax, caspase-3 and caspase-9, indicating mitochondrial pathway-mediated apoptosis. In vitro, As exposure induced hepatocyte morphological changes, reactive oxygen species (ROS) production, and apoptosis. Additionally, mapk1 and mapk3 (ERK pathway) were downregulated both in vivo and in vitro, while mapk14a (P38 pathway), mapk8b and mapk9 (JNK pathway) were upregulated exclusively in hepatocytes. Furthermore, n-acetyl cysteine (NAC) attenuated As-induced apoptosis and modulated the expression of MAPK signaling pathway genes, including mapk3 and mapk8b, suggesting that As-induced oxidative stress regulates apoptosis via the MAPK signaling pathway. In contrast, phenylbutyric acid (PBA) was ineffective in preventing apoptosis. Overall, these results demonstrate that As induces endogenous apoptosis through oxidative stress and the MAPK signaling pathway in marine medaka.

砷（As）对水生生物的危害已被广泛认识；然而，其对海鱼细胞凋亡的毒理学影响尚未得到充分探讨。本研究通过对海洋medaka肝脏细胞系在体内暴露于50或500 mg/kg AsIII（以NaAsO2形式）超过28天，以及在体外暴露于50 - 750 μg/L AsIII 48小时的影响进行研究，以阐明其毒性及其潜在的分子机制。在体内，As在肝组织中显著积累（为对照组的1.79倍），引起肝脏病变和细胞凋亡增加（分别为4.85±0.56%和9.29±1.82%）。基因表达分析显示bcl2l1下调，bax、caspase-3和caspase-9上调，提示线粒体途径介导的细胞凋亡。在体外，砷暴露诱导肝细胞形态改变、活性氧（ROS）产生和细胞凋亡。此外，mapk1和mapk3 （ERK通路）在体内和体外均下调，而mapk14a （P38通路）、mapk8b和mapk9 （JNK通路）仅在肝细胞中上调。此外，n-乙酰半胱氨酸（NAC）可减弱砷诱导的细胞凋亡，并调节MAPK信号通路基因mapk3和mapk8b的表达，表明砷诱导的氧化应激通过MAPK信号通路调控细胞凋亡。而苯基丁酸（PBA）对细胞凋亡无抑制作用。综上所述，这些结果表明，As通过氧化应激和MAPK信号通路诱导海洋medaka内源性细胞凋亡。

{"title":"Arsenite-induced liver apoptosis via oxidative stress and the MAPK signaling pathway in marine medaka","authors":"Jiangtian Lin , Ting Zhang , Li Zhang","doi":"10.1016/j.aquatox.2024.107226","DOIUrl":"10.1016/j.aquatox.2024.107226","url":null,"abstract":"<div><div>Arsenic (As) is widely recognized for its hazards to aquatic organisms; however, its toxicological impacts on apoptosis in marine fish remain inadequately explored. This study investigated the effects of <em>in vivo</em> dietary exposure to 50 or 500 mg/kg AsIII (as NaAsO<sub>2</sub>) over 28 days in marine medaka, alongside <em>in vitro</em> exposure to 50–750 μg/L AsIII for 48 h in a hepatic cell line derived from marine medaka, to elucidate the toxicity and underlying molecular mechanisms. <em>In vivo</em>, As significantly accumulated in liver tissue (1.79-fold compared to the control), causing hepatic lesions and increased apoptosis (4.85 ± 0.56 % and 9.29 ± 1.82 %, respectively). Gene expression analysis showed downregulation of <em>bcl2l1</em> and upregulation of <em>bax, caspase-3</em> and <em>caspase-9</em>, indicating mitochondrial pathway-mediated apoptosis. <em>In vitro</em>, As exposure induced hepatocyte morphological changes, reactive oxygen species (ROS) production, and apoptosis. Additionally, <em>mapk1</em> and <em>mapk3</em> (ERK pathway) were downregulated both <em>in vivo</em> and <em>in vitro</em>, while <em>mapk14a</em> (P38 pathway), <em>mapk8b</em> and <em>mapk9</em> (JNK pathway) were upregulated exclusively in hepatocytes. Furthermore, n-acetyl cysteine (NAC) attenuated As-induced apoptosis and modulated the expression of MAPK signaling pathway genes, including <em>mapk3</em> and <em>mapk8b</em>, suggesting that As-induced oxidative stress regulates apoptosis via the MAPK signaling pathway. In contrast, phenylbutyric acid (PBA) was ineffective in preventing apoptosis. Overall, these results demonstrate that As induces endogenous apoptosis through oxidative stress and the MAPK signaling pathway in marine medaka.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107226"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for low bioavailability of dietary nanoparticulate cerium in a freshwater food chain 淡水食物链中膳食纳米粒铈生物利用度低的证据

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107228

Lisa A. Golding , Paul Callaghan , Brad M. Angel , Graeme E. Batley , Grant Griffiths , An Nguyen , Tom Cresswell

Radioactive ¹⁴¹Ce in ionic (I-Ce), nano (N-Ce, 11 ± 9 nm mean primary particle size ± standard deviation) and micron-sized (M-Ce, 530 ± 440 µm) forms associated with natural and artificial diets in natural river water and synthetic freshwater were used to measure the real-time biokinetics of dietary ¹⁴¹Ce assimilation in a freshwater food chain. The model food chain consisted of microalgae (Raphidocelis subcapitata), snails (Potamopyrgus antipodarum) and prawns (Macrobrachium australiense). Pulse-chase experiments showed that 91–100 % of all forms of cerium associated with all diets and water types were eliminated from the digestive system of the snail and prawn within 24 h, with no detectable cerium assimilation. The prawn and snail median elimination times (ET50) and elimination rates (Ke) for all cerium forms ranged from 0.05 to 1.7 d, and 30 to >100 % per d, respectively. The pulse-chase results were supported by the autoradiographic evidence for N-Ce and M-Ce that confirmed no detectable assimilation and translocation within the tissue of the prawn over time. In contrast, the more soluble I-Ce was found to be associated in low quantities with the hepatopancreas in the prawn confirming that the lack of dissolution by N-Ce and M-Ce in the digestive environment of these organisms makes these forms less bioavailable. In addition, hetero-agglomeration of N-Ce and M-Ce resulted in particles that did not dissociate in digestive fluids and were too large to be assimilated thereby making them non-bioavailable. Based on the results from this study and from the literature review, the risk of N-Ce biomagnification and chronic dietary toxicity in freshwater ecosystems is no greater than the risk associated with M-Ce or I-Ce.

采用天然河水和合成淡水中与天然和人工饲料相关的离子（I-Ce）、纳米（N-Ce，平均初级粒径为11±9 nm±标准差）和微米（m - ce, 530±440µm）形式的放射性141Ce，测量了淡水食物链中饲料中141Ce同化的实时生物动力学。模型食物链由微藻（Raphidocelis subcapitata）、蜗牛（Potamopyrgus antipodarum）和对虾（Macrobrachium australiense）组成。脉冲追踪实验表明，在24 h内，与所有饲料和水类型相关的91 - 100%的各种形式的铈从蜗牛和对虾的消化系统中被消除，没有检测到铈的同化。对虾和蜗牛对所有形式铈的中位消除时间（ET50）和消除率（Ke）分别为0.05 ~ 1.7 d和30 ~ 100% / d。脉冲追踪结果得到了N-Ce和M-Ce的放射自显影证据的支持，这些证据证实，随着时间的推移，对虾的组织内没有可检测到的同化和移位。相比之下，在对虾的肝胰脏中发现了较易溶解的低量I-Ce，这证实了在这些生物体的消化环境中缺乏N-Ce和M-Ce的溶解使得这些形式的生物利用度较低。此外，N-Ce和M-Ce的异团聚导致颗粒不能在消化液中解离，并且太大而无法被吸收，从而使它们不能被生物利用。根据本研究结果和文献综述，淡水生态系统中N-Ce生物放大和慢性膳食毒性的风险并不大于与M-Ce或I-Ce相关的风险。

{"title":"Evidence for low bioavailability of dietary nanoparticulate cerium in a freshwater food chain","authors":"Lisa A. Golding , Paul Callaghan , Brad M. Angel , Graeme E. Batley , Grant Griffiths , An Nguyen , Tom Cresswell","doi":"10.1016/j.aquatox.2024.107228","DOIUrl":"10.1016/j.aquatox.2024.107228","url":null,"abstract":"<div><div>Radioactive <sup>141</sup>Ce in ionic (I-Ce), nano (N-Ce, 11 ± 9 nm mean primary particle size ± standard deviation) and micron-sized (M-Ce, 530 ± 440 µm) forms associated with natural and artificial diets in natural river water and synthetic freshwater were used to measure the real-time biokinetics of dietary <sup>141</sup>Ce assimilation in a freshwater food chain. The model food chain consisted of microalgae (<em>Raphidocelis subcapitata</em>), snails (<em>Potamopyrgus antipodarum</em>) and prawns (<em>Macrobrachium australiense</em>). Pulse-chase experiments showed that 91–100 % of all forms of cerium associated with all diets and water types were eliminated from the digestive system of the snail and prawn within 24 h, with no detectable cerium assimilation. The prawn and snail median elimination times (ET50) and elimination rates (Ke) for all cerium forms ranged from 0.05 to 1.7 d, and 30 to >100 % per d, respectively. The pulse-chase results were supported by the autoradiographic evidence for N-Ce and M-Ce that confirmed no detectable assimilation and translocation within the tissue of the prawn over time. In contrast, the more soluble I-Ce was found to be associated in low quantities with the hepatopancreas in the prawn confirming that the lack of dissolution by N-Ce and M-Ce in the digestive environment of these organisms makes these forms less bioavailable. In addition, hetero-agglomeration of N-Ce and M-Ce resulted in particles that did not dissociate in digestive fluids and were too large to be assimilated thereby making them non-bioavailable. Based on the results from this study and from the literature review, the risk of N-Ce biomagnification and chronic dietary toxicity in freshwater ecosystems is no greater than the risk associated with M-Ce or I-Ce.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107228"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender effects of nanoplastics and emerging contaminants mixtures in Mytilus galloprovincialis 纳米塑料和新出现的污染物混合物在紫贻贝中的性别效应

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107219

J.M. Gonçalves , M. Benedetti , G. d'Errico , F. Regoli , M.J. Bebianno

The reproduction of mussels occurs within the water column, and if gametogenesis is successful, gametes are exposed to the surrounding contaminants. Nanoplastics and other emerging contaminants have been gaining vast attention; however, their effects on the reproductive tissues of mussels with sex differentiation are scarce. Here, the effects of polystyrene nanoparticles (50 nm; 10 µg/L), the cytotoxic drug 5-fluorouracil (10 ng/L), and a mixture of the two were evaluated in the gonads of Mytilus galloprovincialis after a 21-day exposure for a multi-biomarker assessment, and after 28 days for the accumulation of nanoplastics. The effects on the activity of superoxide dismutase, catalase, glutathione-S-transferase, and lipid peroxidation were evaluated. Moreover, synergistic and antagonistic interactions in the mixture were calculated. A weight of evidence model was also used to elaborate on the hazardous level of biomarker results relative to polystyrene nanoparticles alone and in the mixture. The accumulation of nanoplastics appeared gender and time-specific, with females mostly compromised. According to the data set, a synergistic interaction between the cytotoxic drug and the nanoplastics makes the combination far more dangerous than individual stressors. The Weight Of Evidence model also confirms that females are more compromised at chronic exposure times than males. This study shows that the uptake, fate, and impact of emerging contaminants of concern can be significantly influenced by sex.

贻贝的繁殖发生在水柱中，如果配子发生成功，配子就会暴露在周围的污染物中。纳米塑料和其他新兴污染物已经引起了广泛的关注；然而，它们对具有性别分化的贻贝生殖组织的影响很少。在这里，聚苯乙烯纳米颗粒(50 nm；10µg/L)，细胞毒性药物5-氟尿嘧啶（10 ng/L），以及两者的混合物，在暴露21天后进行多生物标志物评估，并在28天后进行纳米塑料积累评估。评价了其对超氧化物歧化酶、过氧化氢酶、谷胱甘肽- s -转移酶和脂质过氧化活性的影响。此外，还计算了混合物中的协同和拮抗相互作用。证据权重模型还用于详细说明相对于单独的聚苯乙烯纳米颗粒和混合物中的生物标志物结果的危险水平。纳米塑料的积累具有性别和时间特异性，以雌性为主。根据数据集，细胞毒性药物和纳米塑料之间的协同相互作用使得这种组合比单个压力源要危险得多。证据权重模型还证实，女性在长期暴露时间下比男性更容易受到损害。这项研究表明，新出现的污染物的吸收、命运和影响可能受到性别的显著影响。

{"title":"Gender effects of nanoplastics and emerging contaminants mixtures in Mytilus galloprovincialis","authors":"J.M. Gonçalves , M. Benedetti , G. d'Errico , F. Regoli , M.J. Bebianno","doi":"10.1016/j.aquatox.2024.107219","DOIUrl":"10.1016/j.aquatox.2024.107219","url":null,"abstract":"<div><div>The reproduction of mussels occurs within the water column, and if gametogenesis is successful, gametes are exposed to the surrounding contaminants. Nanoplastics and other emerging contaminants have been gaining vast attention; however, their effects on the reproductive tissues of mussels with sex differentiation are scarce. Here, the effects of polystyrene nanoparticles (50 nm; 10 µg/L), the cytotoxic drug 5-fluorouracil (10 ng/L), and a mixture of the two were evaluated in the gonads of <em>Mytilus galloprovincialis</em> after a 21-day exposure for a multi-biomarker assessment, and after 28 days for the accumulation of nanoplastics. The effects on the activity of superoxide dismutase, catalase, glutathione-<em>S</em>-transferase, and lipid peroxidation were evaluated. Moreover, synergistic and antagonistic interactions in the mixture were calculated. A weight of evidence model was also used to elaborate on the hazardous level of biomarker results relative to polystyrene nanoparticles alone and in the mixture. The accumulation of nanoplastics appeared gender and time-specific, with females mostly compromised. According to the data set, a synergistic interaction between the cytotoxic drug and the nanoplastics makes the combination far more dangerous than individual stressors. The Weight Of Evidence model also confirms that females are more compromised at chronic exposure times than males. This study shows that the uptake, fate, and impact of emerging contaminants of concern can be significantly influenced by sex.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107219"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing predictive models for assessing LC50 of organic contaminants in Gammarus species using interpretable structural parameters 利用可解释的结构参数建立预测模型来评估Gammarus物种中有机污染物的LC50

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107235

Mehran Karimi , Eskandar Kolvari , Mohammad Hossein Keshavarz , Nadiya Koukabi

Gammarus species are crucial indicators of environmental health, making them important for ecological studies and water quality assessments. They offer a wide range of specific responses regarding the median lethal concentration (LC₅₀) of organic compounds. This research presents four predictive models to determine the LC₅₀ of chemicals impacting selected gammarid amphipods: Gammarus lacustris, Gammarus fasciatus, Gammarus pulex, and Gammarus pseudolimnaeus. These species are recognized for their sensitivity to various pollutants and are among the most sensitive aquatic invertebrates. The new models provide straightforward methods for estimating the pLC₅₀ (−log LC₅₀/molecular weight) of various organic compounds based on interpretable structural parameters including the number of effective functional groups, the types of atoms present, and various structural characteristics of organic molecules. This study aims to leverage the largest available experimental dataset compared to prior quantitative structure–activity relationship (QSAR) models for these gammarid amphipods. The dataset contained toxicity data for 91 compounds affecting Gammarus fasciatus, 50 for Gammarus lacustris, and 48 each for Gammarus pseudolimnaeus and Gammarus pulex, aligning with comparative QSAR models. External datasets included 13 compounds for Gammarus fasciatus, 2 for Gammarus lacustris, and 6 for Gammarus pseudolimnaeus. Efforts focus on using interpretable structural parameters of organic compounds rather than computer-based descriptors, as outlined in the existing QSAR models. For the species G. fasciatus, G. lacustris, G. pseudolimnaeus, and G. pulex, the R² ratios for the new models versus the best QSAR models are 0.915/0.728, 0.955/0.747, 0.976/0.769, and 0.970/0.768, respectively. The higher R² values in the new models demonstrate greater reliability and robustness in capturing the data's underlying relationships.

该物种是环境健康的重要指标，对生态研究和水质评价具有重要意义。它们对有机化合物的中位致死浓度（LC50）提供了广泛的特定响应。本研究提出了四种预测模型，以确定影响Gammarus lacustris， Gammarus fasciatus， Gammarus pulex和Gammarus pseudolimnaeus的化学物质的LC50。这些物种因其对各种污染物的敏感性而被认为是最敏感的水生无脊椎动物之一。新模型提供了基于可解释的结构参数（包括有效官能团的数量、存在的原子类型和有机分子的各种结构特征）来估计各种有机化合物的pLC50（−log LC50/分子量）的直接方法。本研究的目的是利用最大的可用实验数据集，与之前的定量结构-活性关系（QSAR）模型相比，这些双足类动物。该数据集包含91种影响筋膜Gammarus fasciatus的化合物的毒性数据，50种影响湖泊Gammarus， 48种影响伪滨Gammarus和细尾Gammarus pulex，与比较QSAR模型一致。外部数据集包括13个fasciatus Gammarus化合物，2个湖泊Gammarus化合物和6个伪滨虾Gammarus化合物。努力的重点是使用有机化合物的可解释结构参数，而不是现有QSAR模型中概述的基于计算机的描述符。新模型与最佳QSAR模型的R²比分别为0.915/0.728、0.955/0.747、0.976/0.769和0.970/0.768。新模型中较高的R²值表明，在捕捉数据的潜在关系方面具有更高的可靠性和鲁棒性。

{"title":"Developing predictive models for assessing LC50 of organic contaminants in Gammarus species using interpretable structural parameters","authors":"Mehran Karimi , Eskandar Kolvari , Mohammad Hossein Keshavarz , Nadiya Koukabi","doi":"10.1016/j.aquatox.2025.107235","DOIUrl":"10.1016/j.aquatox.2025.107235","url":null,"abstract":"<div><div><em>Gammarus</em> species are crucial indicators of environmental health, making them important for ecological studies and water quality assessments. They offer a wide range of specific responses regarding the median lethal concentration (<em>LC<sub>50</sub></em>) of organic compounds. This research presents four predictive models to determine the <em>LC<sub>50</sub></em> of chemicals impacting selected gammarid amphipods: <em>Gammarus lacustris, Gammarus fasciatus, Gammarus pulex</em>, and <em>Gammarus pseudolimnaeus</em>. These species are recognized for their sensitivity to various pollutants and are among the most sensitive aquatic invertebrates. The new models provide straightforward methods for estimating the <em>pLC<sub>50</sub></em> (−log <em>LC<sub>50</sub></em>/molecular weight) of various organic compounds based on interpretable structural parameters including the number of effective functional groups, the types of atoms present, and various structural characteristics of organic molecules. This study aims to leverage the largest available experimental dataset compared to prior quantitative structure–activity relationship (QSAR) models for these gammarid amphipods. The dataset contained toxicity data for 91 compounds affecting <em>Gammarus fasciatus</em>, 50 for <em>Gammarus lacustris</em>, and 48 each for <em>Gammarus pseudolimnaeus</em> and <em>Gammarus pulex</em>, aligning with comparative QSAR models. External datasets included 13 compounds for <em>Gammarus fasciatus</em>, 2 for <em>Gammarus lacustris</em>, and 6 for <em>Gammarus pseudolimnaeus</em>. Efforts focus on using interpretable structural parameters of organic compounds rather than computer-based descriptors, as outlined in the existing QSAR models. For the species G<em>. fasciatus</em>, G<em>. lacustris</em>, G<em>. pseudolimnaeus</em>, and G<em>. pulex</em>, the R² ratios for the new models versus the best QSAR models are 0.915/0.728, 0.955/0.747, 0.976/0.769, and 0.970/0.768, respectively. The higher R² values in the new models demonstrate greater reliability and robustness in capturing the data's underlying relationships.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107235"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tributyl phosphate inhibits neurogenesis and motor functions during embryonic development in zebrafish 磷酸三丁酯抑制斑马鱼胚胎发育过程中的神经发生和运动功能。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107203

Gourav Chakraborty , Bhagyashri Joshi , Kedar Ahire , Chinmoy Patra

Tributyl phosphate (TBP), an organophosphate ester (OPE), is heavily used as a solvent in chemical industries, a plasticizer, and to extract radioactive molecules. Thus, widespread uses of TBP in industrialized countries led to the release of TBP and its metabolites, dibutyl phosphate (DBP) and monobutyl phosphate (MBP), in the environment and were detected in human samples. Accumulating these OPEs over time in humans and aquatic animals may develop toxicological effects. The reports also say TBP passes through the mother-fetal transmission route and may affect embryonic development. However, the impact of TBP and its metabolites on vertebrate development has been poorly studied. Ex-utero development, high fecundity, and optical transparency make the zebrafish a preferred model for toxicological evaluation. Thus, we aim to explore the toxic effects of TBP and its metabolites on aquatic animals using zebrafish as a model organism. Embryos in the chorion were incubated in 10–60 µM test chemicals from 6 to 48 h post fertilization (hpf), and analyzed the adverse effects on embryos. Our study found that 10–20 µM TBP inhibits neural growth, resulting in decreased spontaneous movement frequency and locomotive behavior without altering the overall embryonic growth and muscle functions. In contrast, DBP-treated embryos showed increased spontaneous movement frequency without changing the motor neuron growth and locomotive behavior. Further, in higher concentrations, TBP is teratogenic, and DBP is lethal to the embryos. Altogether, we found that TBP inhibits neurogenesis and motor behavior; however, its metabolite DBP is neuroexcitatory in zebrafish embryos.

磷酸三丁酯（TBP）是一种有机磷酸酯（OPE），在化学工业中被广泛用作溶剂、增塑剂和提取放射性分子。因此，工业化国家对TBP的广泛使用导致TBP及其代谢物磷酸二丁酯（DBP）和磷酸一丁酯（MBP）在环境中释放，并在人类样本中检测到。在人类和水生动物体内长期积累这些OPEs可能会产生毒理学效应。报告还说，TBP通过母婴传播途径，可能影响胚胎发育。然而，TBP及其代谢物对脊椎动物发育的影响研究甚少。体外发育，高繁殖力和光学透明性使斑马鱼成为毒理学评估的首选模型。因此，我们的目的是探讨TBP及其代谢物对水生动物的毒性作用，以斑马鱼为模式生物。受精后6 ~ 48 h，绒毛膜内胚胎在10 ~ 60µM的试验化学品中孵育，分析其对胚胎的不良影响。我们的研究发现，10-20µM TBP抑制神经生长，导致自发运动频率和运动行为降低，但不改变整体胚胎生长和肌肉功能。相比之下，dbp处理的胚胎在不改变运动神经元生长和运动行为的情况下，自发运动频率增加。此外，在较高浓度下，TBP会致畸，DBP对胚胎是致命的。总之，我们发现TBP抑制神经发生和运动行为；然而，其代谢物DBP在斑马鱼胚胎中具有神经兴奋性。

{"title":"Tributyl phosphate inhibits neurogenesis and motor functions during embryonic development in zebrafish","authors":"Gourav Chakraborty , Bhagyashri Joshi , Kedar Ahire , Chinmoy Patra","doi":"10.1016/j.aquatox.2024.107203","DOIUrl":"10.1016/j.aquatox.2024.107203","url":null,"abstract":"<div><div>Tributyl phosphate (TBP), an organophosphate ester (OPE), is heavily used as a solvent in chemical industries, a plasticizer, and to extract radioactive molecules. Thus, widespread uses of TBP in industrialized countries led to the release of TBP and its metabolites, dibutyl phosphate (DBP) and monobutyl phosphate (MBP), in the environment and were detected in human samples. Accumulating these OPEs over time in humans and aquatic animals may develop toxicological effects. The reports also say TBP passes through the mother-fetal transmission route and may affect embryonic development. However, the impact of TBP and its metabolites on vertebrate development has been poorly studied. <em>Ex-utero</em> development, high fecundity, and optical transparency make the zebrafish a preferred model for toxicological evaluation. Thus, we aim to explore the toxic effects of TBP and its metabolites on aquatic animals using zebrafish as a model organism. Embryos in the chorion were incubated in 10–60 µM test chemicals from 6 to 48 h post fertilization (hpf), and analyzed the adverse effects on embryos. Our study found that 10–20 µM TBP inhibits neural growth, resulting in decreased spontaneous movement frequency and locomotive behavior without altering the overall embryonic growth and muscle functions. In contrast, DBP-treated embryos showed increased spontaneous movement frequency without changing the motor neuron growth and locomotive behavior. Further, in higher concentrations, TBP is teratogenic, and DBP is lethal to the embryos. Altogether, we found that TBP inhibits neurogenesis and motor behavior; however, its metabolite DBP is neuroexcitatory in zebrafish embryos.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107203"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential effects of corn cob biochar on mitigating pendimethalin-induced toxicity in Nile tilapia (Oreochromis niloticus): Effects on hematological, biochemical, antioxidant-immune parameters, and histopathological alterations 玉米芯生物炭对尼罗罗非鱼（Oreochromis niloticus）减轻二甲氧甲烷引起的毒性的潜在影响：对血液、生化、抗氧化免疫参数和组织病理学改变的影响。

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107213

Rasha M. Reda , Eman M. Zaki , Ahmed A.A. Aioub , Mohamed M.M. Metwally , Fatma Mahsoub

This study aims to examine the restorative impact of corn cob biochar (CCB) on pendimethalin (PMD)-induced toxicity in Oreochromis niloticus. Fish were divided into four groups: the first control group without treatment, the second group (CCB) exposed to 10 g CCB/L, the third group (PDM) exposed to 0.355 mg PDM/L, and the fourth group (PDM+ CCB) receiving both 0.355 mg PDM/L and 10 g CCB/L for 30 days. PDM exposure resulted in behavioral alterations, low survival rate (73.33 %), hematological and biochemical impairments, increased oxidative stress, suppressed immunity, and histopathological damage in gill, liver, and brain tissues. Co-treatment with CCB significantly alleviated these effects, as evidenced by improved survival rate (88.88 %), hematological, biochemical, and antioxidant-immune parameters and reduced histopathological alterations. In conclusion, CCB demonstrated a promising potential to mitigate PDM-induced toxicity in O. niloticus by enhancing physiological, biochemical, and histological resilience.

本研究旨在研究玉米芯生物炭（CCB）对二甲甲烷（PMD）诱导的nilochromis毒性的恢复作用。鱼被分为四组：第一组未处理，第二组（CCB）暴露于10 g CCB/L，第三组（PDM）暴露于0.355 mg PDM/L，第四组（PDM+ CCB）同时接受0.355 mg PDM/L和10 g CCB/L，持续30天。PDM暴露导致行为改变、低存活率（73.33%）、血液学和生化损伤、氧化应激增加、免疫抑制以及鳃、肝和脑组织的组织病理学损伤。与CCB联合治疗显著缓解了这些影响，证明了生存率（88.88%）、血液学、生化和抗氧化免疫参数的提高以及组织病理学改变的减少。综上所述，CCB通过增强niloticus的生理、生化和组织学恢复力，显示出减轻pdm诱导的毒性的潜力。

{"title":"The potential effects of corn cob biochar on mitigating pendimethalin-induced toxicity in Nile tilapia (Oreochromis niloticus): Effects on hematological, biochemical, antioxidant-immune parameters, and histopathological alterations","authors":"Rasha M. Reda , Eman M. Zaki , Ahmed A.A. Aioub , Mohamed M.M. Metwally , Fatma Mahsoub","doi":"10.1016/j.aquatox.2024.107213","DOIUrl":"10.1016/j.aquatox.2024.107213","url":null,"abstract":"<div><div>This study aims to examine the restorative impact of corn cob biochar (CCB) on pendimethalin (PMD)-induced toxicity in <em>Oreochromis niloticus</em>. Fish were divided into four groups: the first control group without treatment, the second group (CCB) exposed to 10 g CCB/L, the third group (PDM) exposed to 0.355 mg PDM/L, and the fourth group (PDM+ CCB) receiving both 0.355 mg PDM/L and 10 g CCB/L for 30 days. PDM exposure resulted in behavioral alterations, low survival rate (73.33 %), hematological and biochemical impairments, increased oxidative stress, suppressed immunity, and histopathological damage in gill, liver, and brain tissues. Co-treatment with CCB significantly alleviated these effects, as evidenced by improved survival rate (88.88 %), hematological, biochemical, and antioxidant-immune parameters and reduced histopathological alterations. In conclusion, CCB demonstrated a promising potential to mitigate PDM-induced toxicity in <em>O. niloticus</em> by enhancing physiological, biochemical, and histological resilience.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107213"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intelligent consensus-based predictions of early life stage toxicity in fish tested in compliance with OECD Test Guideline 210 根据经合组织测试指南210对鱼类生命早期毒性进行基于智能共识的预测

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107216

Souvik Pore , Alexia Pelloux , Anders Bergqvist , Mainak Chatterjee , Kunal Roy

Early life stage (ELS) toxicity testing in fish is a crucial test procedure used to evaluate the long-term effects of a wide range of chemicals, including pesticides, industrial chemicals, pharmaceuticals, and food additives. This test is particularly important for screening and prioritizing thousands of chemicals under the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH) regulation. In silico methods can be used to estimate the toxicity of a chemical when no experimental data is available and to reduce the cost, time, and resources involved in the experimentation process. In the present study, we developed predictive Quantitative Structure-Activity Relationship (QSAR) models to assess chronic effects of chemicals on ELS in fish. Toxicity data for ELS in fish was collected from two different sources, i.e. J-CHECK and eChemPortal, which contain robust study summaries of experimental studies performed according to OECD Test Guideline 210. The collected data included two types of endpoints – the No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC), which were utilized to develop the QSAR models. Six different partial least squares (PLS) models with various descriptor combinations were created for both endpoints. These models were then employed for intelligent consensus-based prediction to enhance predictability for unknown chemicals. Among these models, the consensus model – 3 (Q²_F1 = 0.71, Q²_F2 = 0.71) and individual model – 3 (Q²_F1 = 0.80, Q²_F2 = 0.79) exhibited most promising results for both the NOEC and LOEC endpoints. Furthermore, these models were validated experimentally using experimental data from nine different industrial chemicals provided by Global Product Compliance (Europe) AB. Lastly, the models were used to screen and prioritize chemicals obtained from the Pesticide Properties (PPDB) and DrugBank databases.

鱼类的早期生命阶段毒性测试是一项关键的测试程序，用于评估各种化学品的长期影响，包括杀虫剂、工业化学品、药品和食品添加剂。该测试对于根据化学品注册，评估，授权和限制（REACH）法规筛选和优先考虑数千种化学品尤为重要。在没有实验数据的情况下，计算机方法可用于估计化学物质的毒性，并减少实验过程中涉及的成本、时间和资源。在本研究中，我们建立了预测定量构效关系（QSAR）模型来评估化学物质对鱼类ELS的慢性影响。鱼类中ELS的毒性数据是从两个不同的来源收集的，即J-CHECK和eChemPortal，其中包含根据经合组织测试指南210进行的实验研究的可靠研究摘要。收集的数据包括两种类型的终点-无观测效应浓度（NOEC）和最低观测效应浓度（LOEC），用于建立QSAR模型。为两个端点创建了具有不同描述符组合的六个不同的偏最小二乘（PLS）模型。然后将这些模型用于基于共识的智能预测，以提高对未知化学物质的可预测性。在这些模型中，共识模型- 3 （Q2F1 = 0.71, Q2F2 = 0.71）和个体模型- 3 （Q2F1 = 0.80, Q2F2 = 0.79）对NOEC和LOEC端点都显示出最有希望的结果。此外，使用Global Product Compliance (Europe) AB提供的九种不同工业化学品的实验数据对这些模型进行了实验验证。最后，这些模型用于筛选和优先考虑从农药属性（PPDB）和DrugBank数据库中获得的化学品。

{"title":"Intelligent consensus-based predictions of early life stage toxicity in fish tested in compliance with OECD Test Guideline 210","authors":"Souvik Pore , Alexia Pelloux , Anders Bergqvist , Mainak Chatterjee , Kunal Roy","doi":"10.1016/j.aquatox.2024.107216","DOIUrl":"10.1016/j.aquatox.2024.107216","url":null,"abstract":"<div><div>Early life stage (ELS) toxicity testing in fish is a crucial test procedure used to evaluate the long-term effects of a wide range of chemicals, including pesticides, industrial chemicals, pharmaceuticals, and food additives. This test is particularly important for screening and prioritizing thousands of chemicals under the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH) regulation. In silico methods can be used to estimate the toxicity of a chemical when no experimental data is available and to reduce the cost, time, and resources involved in the experimentation process. In the present study, we developed predictive Quantitative Structure-Activity Relationship (QSAR) models to assess chronic effects of chemicals on ELS in fish. Toxicity data for ELS in fish was collected from two different sources, i.e. J-CHECK and eChemPortal, which contain robust study summaries of experimental studies performed according to OECD Test Guideline 210. The collected data included two types of endpoints – the No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC), which were utilized to develop the QSAR models. Six different partial least squares (PLS) models with various descriptor combinations were created for both endpoints. These models were then employed for intelligent consensus-based prediction to enhance predictability for unknown chemicals. Among these models, the consensus model – 3 (Q<sup>2</sup><sub>F1</sub> = 0.71, Q<sup>2</sup><sub>F2</sub> = 0.71) and individual model – 3 (Q<sup>2</sup><sub>F1</sub> = 0.80, Q<sup>2</sup><sub>F2</sub> = 0.79) exhibited most promising results for both the NOEC and LOEC endpoints. Furthermore, these models were validated experimentally using experimental data from nine different industrial chemicals provided by Global Product Compliance (Europe) AB. Lastly, the models were used to screen and prioritize chemicals obtained from the Pesticide Properties (PPDB) and DrugBank databases.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107216"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurodevelopmental toxicity and mechanism of action of monoethylhexyl phthalate (MEHP) in the developing zebrafish (Danio rerio) 邻苯二甲酸一乙基己基酯（MEHP）对发育中的斑马鱼的神经发育毒性及作用机制

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107230

Chaoqiong Wu , Guanghua Mao , Xiang Ji , Yao Chen , Xin Geng , Emmanuel Sunday Okeke , Yangyang Ding , Liuqing Yang , Xiangyang Wu , Weiwei Feng

Monoethylhexyl phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl) phthalate (DEHP), the most prevalent phthalate plasticiser globally. It has been demonstrated that MEHP exerts more potent toxic effects than DEHP. Nevertheless, the full extent of the toxicity of MEHP to neurodevelopmental organisms remains unclear. Accordingly, the present study was designed to investigate the neurodevelopmental toxicity of MEHP exposure and the underlying molecular mechanisms. Zebrafish juveniles were exposed to different concentrations of MEHP (7.42, 14.84, 29.68 and 74.2 μg/L) for a period of four weeks. Immunohistological evidence indicated that MEHP exposure resulted in oxidative stress and apoptosis in the developing zebrafish brain. Subsequently, the neurobehaviour of zebrafish larvae was evaluated, and it was determined that MEHP significantly disrupted their locomotor capacity, motor vigor, and social conduct. Furthermore, HE staining revealed damage to brain neurons, which may be linked to impaired synthesis and conduction of inter-synaptic neurotransmitters. Transcriptomic analyses indicated that MEHP may affect the expression levels of genes in the P53 signalling pathway and signalling pathways related to the development of the nervous system. This results in impaired functions, including nerve conduction and neuronal development. Additionally, it induces oxidative stress, which leads to significant brain cell apoptosis and, ultimately, neurotoxicity in developing zebrafish.

邻苯二甲酸一乙基己基酯（MEHP）是邻苯二甲酸二（2-乙基己基）酯（DEHP）的主要代谢物，邻苯二甲酸二酯（DEHP）是全球最普遍的邻苯二甲酸酯增塑剂。已经证明，MEHP比DEHP具有更强的毒性作用。然而，MEHP对神经发育生物体的毒性程度仍不清楚。因此，本研究旨在探讨MEHP暴露的神经发育毒性及其潜在的分子机制。将斑马鱼幼鱼暴露于不同浓度的MEHP（7.42、14.84、29.68和74.2 μg/L）中4周。免疫组织学证据表明，MEHP暴露导致发育中的斑马鱼大脑氧化应激和细胞凋亡。随后，对斑马鱼幼虫的神经行为进行了评估，确定MEHP显著破坏了斑马鱼的运动能力、运动活力和社会行为。此外，HE染色显示脑神经元损伤，这可能与突触间神经递质的合成和传导受损有关。转录组学分析表明，MEHP可能影响P53信号通路及神经系统发育相关信号通路基因的表达水平。这导致功能受损，包括神经传导和神经元发育。此外，它还会诱导氧化应激，从而导致脑细胞凋亡，并最终导致发育中的斑马鱼的神经毒性。

{"title":"Neurodevelopmental toxicity and mechanism of action of monoethylhexyl phthalate (MEHP) in the developing zebrafish (Danio rerio)","authors":"Chaoqiong Wu , Guanghua Mao , Xiang Ji , Yao Chen , Xin Geng , Emmanuel Sunday Okeke , Yangyang Ding , Liuqing Yang , Xiangyang Wu , Weiwei Feng","doi":"10.1016/j.aquatox.2024.107230","DOIUrl":"10.1016/j.aquatox.2024.107230","url":null,"abstract":"<div><div>Monoethylhexyl phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl) phthalate (DEHP), the most prevalent phthalate plasticiser globally. It has been demonstrated that MEHP exerts more potent toxic effects than DEHP. Nevertheless, the full extent of the toxicity of MEHP to neurodevelopmental organisms remains unclear. Accordingly, the present study was designed to investigate the neurodevelopmental toxicity of MEHP exposure and the underlying molecular mechanisms. Zebrafish juveniles were exposed to different concentrations of MEHP (7.42, 14.84, 29.68 and 74.2 μg/L) for a period of four weeks. Immunohistological evidence indicated that MEHP exposure resulted in oxidative stress and apoptosis in the developing zebrafish brain. Subsequently, the neurobehaviour of zebrafish larvae was evaluated, and it was determined that MEHP significantly disrupted their locomotor capacity, motor vigor, and social conduct. Furthermore, HE staining revealed damage to brain neurons, which may be linked to impaired synthesis and conduction of inter-synaptic neurotransmitters. Transcriptomic analyses indicated that MEHP may affect the expression levels of genes in the P53 signalling pathway and signalling pathways related to the development of the nervous system. This results in impaired functions, including nerve conduction and neuronal development. Additionally, it induces oxidative stress, which leads to significant brain cell apoptosis and, ultimately, neurotoxicity in developing zebrafish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107230"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of microplastic accumulation in aquatic environment: Physiological, eco-toxicological, immunological, and neurotoxic effects 水生环境中微塑料积累的影响：生理、生态毒理学、免疫学和神经毒性效应

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2024.107232

Eram Rashid , Syed Makhdoom Hussain , Shafaqat Ali , Muhammad Munir , Abdul Ghafoor , Ebru Yilmaz , Mohammed Ali Alshehri , Danish Riaz , Adan Naeem , Eman Naeem

The presence of microplastics (MPs) in aquatic ecosystem has become a pressing global concern. MPs pose a significant threat to aquatic ecosystems, with devastating consequences for both aquatic life and human health. Notably, freshwater ecosystems are particularly vulnerable to MPs pollution. MPs, characterized by their small size (< 5 mm), have emerged as a ubiquitous environmental pollutant. They exhibit diverse characteristics, including varying sizes, forms, polymer types, and colors. Two distinct categories of MPs exist: primary and secondary. Primary MPs are incorporated into industrial hard materials, cosmetics, and hand cleaners, whereas secondary MPs result from the breakdown of larger plastic products in both terrestrial and marine environments. They enter the environment through various sources, such as household products, clothing, industrial activities, sewage waste and plastic degradation. Aquatic organisms ingest these contaminants, facilitating the transfer of MPs into the food chain and potentially causing severe health problems. This review delves into the bioaccumulation of MPs in fish, highlighting the eco-toxicological, neurological and immunological effects. This review provides an in-depth analysis of innovative solutions for MPs removal and reduction. Finally, we delineate evidence-based strategies to mitigate impacts of MPs, offering valuable insights to inform policy formulations and accelerate the development of sustainable plastic technologies.

微塑料在水生生态系统中的存在已成为一个紧迫的全球性问题。MPs对水生生态系统构成重大威胁，对水生生物和人类健康造成毁灭性后果。值得注意的是，淡水生态系统特别容易受到MPs污染的影响。MPs，其特点是体积小(<；5毫米)，已成为一种无处不在的环境污染物。它们表现出不同的特征，包括不同的尺寸、形状、聚合物类型和颜色。MPs有两种不同的类型：初级和次级。初级MPs被纳入工业硬材料、化妆品和洗手液中，而次级MPs则来自陆地和海洋环境中较大的塑料产品的分解。它们通过各种来源进入环境，如家用产品、服装、工业活动、污水废物和塑料降解。水生生物摄取这些污染物，促进MPs进入食物链，并可能造成严重的健康问题。本文综述了MPs在鱼类体内的生物积累，重点介绍了其生态毒理学、神经学和免疫学效应。这篇综述深入分析了MPs去除和减少的创新解决方案。最后，我们描述了基于证据的策略，以减轻MPs的影响，为政策制定提供有价值的见解，并加速可持续塑料技术的发展。

{"title":"Impacts of microplastic accumulation in aquatic environment: Physiological, eco-toxicological, immunological, and neurotoxic effects","authors":"Eram Rashid , Syed Makhdoom Hussain , Shafaqat Ali , Muhammad Munir , Abdul Ghafoor , Ebru Yilmaz , Mohammed Ali Alshehri , Danish Riaz , Adan Naeem , Eman Naeem","doi":"10.1016/j.aquatox.2024.107232","DOIUrl":"10.1016/j.aquatox.2024.107232","url":null,"abstract":"<div><div>The presence of microplastics (MPs) in aquatic ecosystem has become a pressing global concern. MPs pose a significant threat to aquatic ecosystems, with devastating consequences for both aquatic life and human health. Notably, freshwater ecosystems are particularly vulnerable to MPs pollution. MPs, characterized by their small size (< 5 mm), have emerged as a ubiquitous environmental pollutant. They exhibit diverse characteristics, including varying sizes, forms, polymer types, and colors. Two distinct categories of MPs exist: primary and secondary. Primary MPs are incorporated into industrial hard materials, cosmetics, and hand cleaners, whereas secondary MPs result from the breakdown of larger plastic products in both terrestrial and marine environments. They enter the environment through various sources, such as household products, clothing, industrial activities, sewage waste and plastic degradation. Aquatic organisms ingest these contaminants, facilitating the transfer of MPs into the food chain and potentially causing severe health problems. This review delves into the bioaccumulation of MPs in fish, highlighting the eco-toxicological, neurological and immunological effects. This review provides an in-depth analysis of innovative solutions for MPs removal and reduction. Finally, we delineate evidence-based strategies to mitigate impacts of MPs, offering valuable insights to inform policy formulations and accelerate the development of sustainable plastic technologies.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107232"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CLSSATP: Contrastive learning and self-supervised learning model for aquatic toxicity prediction 水生毒性预测的对比学习和自监督学习模型

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-02-01 DOI: 10.1016/j.aquatox.2025.107244

Ye Lin , Xin Yang , Mingxuan Zhang , Jinyan Cheng , Hai Lin , Qi Zhao

As compound concentrations in aquatic environments increase, the habitat degradation of aquatic organisms underscores the growing importance of studying the impact of chemicals on diverse aquatic populations. Understanding the potential impacts of different chemical substances on different species is a necessary requirement for protecting the environment and ensuring sustainable human development. In this regard, deep learning methods offer significant advantages over traditional experimental approaches in terms of cost, accuracy, and generalization ability. This research introduces CLSSATP, an efficient contrastive self-supervised learning deep neural network prediction model for organic toxicity. The model integrates two modules, a self-supervised learning module using molecular fingerprints for representation, and a contrastive learning module utilizing molecular graphs. Through dual-perspective learning, the model gains clear insights into the structural and property relationships of molecules. The experiment results indicate that our model outperforms comparative methods, demonstrating the effectiveness of our proposed architecture. Moreover, ablation experiments show that the self-supervised module and contrastive learning module respectively provide average performance improvements of 9.43 % and 10.98 % to CLSSATP. Furthermore, by visualizing the representations of our model, we observe that it correctly identifies the substructures that determine the molecular properties, granting itself with interpretability. In conclusion, CLSSATP offers a novel and effective perspective for future research in aquatic toxicity assessment. All of codes and datasets are freely available online at https://github.com/zhaoqi106/CLSSATP.

随着水生环境中化合物浓度的增加，水生生物栖息地的退化凸显了研究化学品对不同水生种群影响的重要性。了解不同化学物质对不同物种的潜在影响是保护环境和确保人类可持续发展的必要条件。在这方面，深度学习方法在成本、准确性和泛化能力方面比传统的实验方法具有显著的优势。本文介绍了一种高效的对比自监督学习深度神经网络预测模型CLSSATP。该模型集成了两个模块，一个是使用分子指纹表示的自监督学习模块，另一个是使用分子图的对比学习模块。通过双视角学习，该模型对分子的结构和性质关系有了清晰的认识。实验结果表明，我们的模型优于比较方法，证明了我们提出的体系结构的有效性。此外，烧蚀实验表明，自监督模块和对比学习模块对CLSSATP的平均性能分别提高了9.43%和10.98%。此外，通过可视化我们模型的表示，我们观察到它正确地识别了决定分子性质的子结构，赋予其可解释性。综上所述，CLSSATP为今后的水生毒性评价研究提供了一个新颖有效的视角。所有的代码和数据集都可以在https://github.com/zhaoqi106/CLSSATP上免费获得。

{"title":"CLSSATP: Contrastive learning and self-supervised learning model for aquatic toxicity prediction","authors":"Ye Lin , Xin Yang , Mingxuan Zhang , Jinyan Cheng , Hai Lin , Qi Zhao","doi":"10.1016/j.aquatox.2025.107244","DOIUrl":"10.1016/j.aquatox.2025.107244","url":null,"abstract":"<div><div>As compound concentrations in aquatic environments increase, the habitat degradation of aquatic organisms underscores the growing importance of studying the impact of chemicals on diverse aquatic populations. Understanding the potential impacts of different chemical substances on different species is a necessary requirement for protecting the environment and ensuring sustainable human development. In this regard, deep learning methods offer significant advantages over traditional experimental approaches in terms of cost, accuracy, and generalization ability. This research introduces CLSSATP, an efficient contrastive self-supervised learning deep neural network prediction model for organic toxicity. The model integrates two modules, a self-supervised learning module using molecular fingerprints for representation, and a contrastive learning module utilizing molecular graphs. Through dual-perspective learning, the model gains clear insights into the structural and property relationships of molecules. The experiment results indicate that our model outperforms comparative methods, demonstrating the effectiveness of our proposed architecture. Moreover, ablation experiments show that the self-supervised module and contrastive learning module respectively provide average performance improvements of 9.43 % and 10.98 % to CLSSATP. Furthermore, by visualizing the representations of our model, we observe that it correctly identifies the substructures that determine the molecular properties, granting itself with interpretability. In conclusion, CLSSATP offers a novel and effective perspective for future research in aquatic toxicity assessment. All of codes and datasets are freely available online at <span><span>https://github.co</span><svg><path></path></svg></span><span><span><em>m/z</em></span><svg><path></path></svg></span><span><span>haoqi106/CLSSATP</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107244"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0