Aquatic Toxicology最新文献_第3页

Chemical induction of settlement and metamorphosis in larvae of Manila clam Ruditapes philippinarum 菲律宾马尼拉蛤幼虫沉降和变态的化学诱导

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-19 DOI: 10.1016/j.aquatox.2026.107725

Jiakang Wang , Zhiyang Cui , Huawang Li , Yancui Chen , Biao Wu , Liqing Zhou , Zhihong Liu , Peizhen Ma , Zhuanzhuan Li , Xiujun Sun

Larval settlement and metamorphosis is a critical biological process governing the recruitment and population dynamics of bivalves, with profound implications for aquaculture sustainability. This study systematically assessed the effects of neurochemical inducers (Serotonin [5-HT], l-DOPA, acetylcholine, choline) and inorganic ions (K^⁺, Ca^²⁺, NH₄^⁺, Mg^²⁺) on larval settlement and metamorphosis in Manila clam Ruditapes philippinarum. Laboratory bioassays demonstrated that 5-HT acted as a potent settlement inducer, eliciting >65% settlement and metamorphosis at concentrations ranging from 10⁻² to 1 μM within 6–24 h. However, it exhibited acute toxicity at 10² μM, resulting in 100% mortality at 72 h. In contrast, l-DOPA showed transient efficacy, achieving 66.3% settlement and metamorphosis at 10 μM within 6 h but lack of induction effects at 48 h. Among ions, K^⁺ (30 mM) and Ca^²⁺ (30–50 mM) significantly enhanced larval settlement and metamorphosis, with the highest induction rates of 41% and 51% at 24 h, respectively, but the elevated concentrations resulted in increased larval mortality (≥ 37.7%). Ammonium chloride (which is usually present in both ionized and more toxic unionized forms, partly dependent on pH) displayed strong toxicity, causing 100% mortality at >20 mM concentrations within 48 h, while Mg²⁺ had the negligible effects on both settlement and survival. These findings highlight the inductive effects of chemical cues on larval settlement and metamorphosis in R. philippinarum, providing practical thresholds (e.g., 1 μM 5-HT with ≤24 h exposure) to optimize hatchery-rearing protocols in aquaculture.

幼虫的定居和变态是控制双壳类动物招募和种群动态的关键生物过程，对水产养殖的可持续性具有深远的影响。本研究系统评估了神经化学诱导剂（血清素[5-HT]、l-DOPA、乙酰胆碱、胆碱）和无机离子（K +、Ca +、NH +、Mg +）对马尼拉蛤幼虫沉降和变形的影响。实验室生物测定表明，5-羟色胺是一种有效的沉降诱导剂，在10 - 2 - 1 μM的浓度范围内，在6 - 24小时内诱发65%的沉降和变态。然而，在10²μM的浓度下，它表现出急性毒性，在72小时内导致100%的死亡率。相比之下，l-DOPA表现出短暂的作用，在10 μM的浓度下，在6小时内达到66.3%的沉降和变态，但在48小时内缺乏诱导作用。K + (30 mM)和Ca 2 + (30 - 50 mM)显著增强了幼虫的沉降和变态，24 h诱导率最高，分别为41%和51%，但浓度升高导致幼虫死亡率升高（≥37.7%）。氯化铵（通常以离子化和毒性更大的游离形式存在，部分依赖于pH）显示出很强的毒性，在20mm浓度下48小时内死亡率为100%，而Mg 2 +对沉降和存活的影响可以忽略不计。这些发现强调了化学线索对菲律宾沙蚕幼虫沉降和变态的诱导作用，为优化水产养殖孵化方案提供了可行的阈值（例如，1 μM - 5-HT≤24 h暴露）。

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引用次数: 0

Impacts of pesticides and vinasse on the composition and functional diversity of aquatic macroinvertebrates exposed in a mesocosm system 农药和酒液对中生态系统中水生大型无脊椎动物组成和功能多样性的影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-18 DOI: 10.1016/j.aquatox.2026.107724

Carina Pereira Cotta , Thandy Junio da Silva Pinto , Diego Ferreira Gomes , Allan Pretti Ogura , Laís Conceição Menezes da Silva , Janaina Braga do Carmo , Cassiana Carolina Montagner , Evaldo Luiz Gaeta Espíndola , Raquel Aparecida Moreira

Modern agriculture plays a significant role in the global economy. However, the continuous use of pesticides and fertilizers raises concerns about aquatic pollution and its effects on indigenous organisms. Thus, this study investigated the impact of the pesticides Regent® 800 WG (active ingredient - a.i. fipronil) and DMA® 806 BR (a.i. 2,4-D), as well as sugarcane vinasse, an ethanol industry byproduct applied in sugarcane crops as fertilizer, individually and in mixtures, on the functional diversity of benthic macroinvertebrates at a mesocosm scale. For that, open-air mesocosms were used, in which the effects of contaminants were evaluated over time (1, 7, 14, 28, 75, and 150 days after contamination). The macroinvertebrate community was monitored using colonization structures, while physicochemical parameters, metals, and pesticides were analyzed throughout the experiment. In the control and 2,4-D-treated mesocosms, collectors, predators, and scrapers were the predominant organisms. In mesocosms contaminated with fipronil and pesticide mixture, predators such as Odonata and Tanypodinae disappeared after contamination. In the pesticide and vinasse mixture treatment, collectors, filter feeders, and scrapers predominated over time. Functional richness and diversity were reduced in the pesticide mixture. Contamination by the mixture of pesticides and vinasse reduced functional diversity, due to the disappearance of predators and tolerant organisms. Therefore, the functionality of the macroinvertebrate fauna may be affected by the application of these contaminants in areas close to sugarcane cultivation. These effects represent a risk to the balance of aquatic ecosystems, compromising trophic chains, ecological functions, and the overall health of the aquatic ecosystem.

现代农业在全球经济中发挥着重要作用。然而，农药和化肥的持续使用引起了人们对水生污染及其对本地生物的影响的关注。因此，本研究调查了农药Regent®800 WG（有效成分- a.i.氟虫腈）和DMA®806 BR (a.i. 2,4- d)，以及甘蔗甘蔗作物作为肥料的乙醇工业副产品甘蔗酒液（单独和混合使用）对中生态尺度下底栖大型无脊椎动物功能多样性的影响。为此，我们使用了露天中生态系统，在该系统中，污染物的影响随时间（污染后1、7、14、28、75和150天）被评估。利用定植结构对大型无脊椎动物群落进行监测，同时对实验过程中的理化参数、金属和农药进行分析。在对照和2,4- d处理的中生态系统中，收集者、捕食者和刮食者是优势生物。在被氟虫腈和农药混合污染的中食性昆虫中，食虫目和踏足目等捕食者在污染后消失。在农药和酒糟混合物处理中，收集器、滤料器和刮刀器占主导地位。混合农药的功能丰富度和多样性降低。由于捕食者和耐受性生物的消失，杀虫剂和酒渣混合物的污染减少了功能多样性。因此，大型无脊椎动物的功能可能会受到这些污染物在甘蔗种植附近地区的影响。这些影响对水生生态系统的平衡构成了威胁，损害了营养链、生态功能和水生生态系统的整体健康。

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引用次数: 0

Quantitative prediction of zebrafish reproductive impairment via androgen receptor-driven adverse outcome pathway 通过雄激素受体驱动的不良后果途径定量预测斑马鱼生殖功能障碍

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-16 DOI: 10.1016/j.aquatox.2026.107720

Xuan Liu , Hongxia Yu , You Song , Wei Shi , Hongling Liu

Exogenous androgenic contaminants have long been of concern and are associated with reproductive impairment in fish. Although Toxcast has screened a large number of compounds using in vitro testing, it is challenging to conduct high-throughput tests for complex effects in vivo and to assess apical endpoints such as reproduction further quantitatively. Therefore, we developed a quantitative prediction method for reproductive dysfunction in zebrafish based on the adverse outcome pathway (AOP) framework. We carried out in vitro androgen activity tests for 16 compounds and a 21-day exposure experiment of androgen receptor (AR) agonism reference chemical 17β-trenbolone (TB) in zebrafish. The results revealed that TB mainly affected the levels of 11-ketotestosterone, estradiol, and vitellogenin in females by mediating AR, and caused abnormal ovarian development and fecundity decline. Then, AOP leading to reproductive dysfunction in female zebrafish (ZqAOP) was assembled and quantified, and it was validated to determine the potential and reliability for chemical extrapolation. Finally, the ZqAOP model was applied for predicting the potential reproductive impairments of eight compounds in environmental concentrations and global surface water samples with AR activity. This study provides a new insight into screening and assessing large numbers of chemicals with potential endocrine-disrupting effects.

外源性雄激素污染物长期以来一直受到关注，并与鱼类生殖功能障碍有关。尽管Toxcast已经使用体外测试筛选了大量化合物，但在体内对复杂效应进行高通量测试以及进一步定量评估生殖等根尖终点是具有挑战性的。因此，我们建立了一种基于不良结果通路（adverse outcome pathway， AOP）框架的斑马鱼生殖功能障碍定量预测方法。我们对16种化合物进行了体外雄激素活性测试，并对斑马鱼进行了雄激素受体（AR）激动剂对照物17β-trenbolone （TB）的21天暴露实验。结果表明，TB主要通过介导AR影响女性体内11-酮睾酮、雌二醇和卵黄原素水平，导致卵巢发育异常和生殖力下降。然后，对导致雌性斑马鱼生殖功能障碍的AOP （ZqAOP）进行组装和量化，并对其进行验证，以确定化学外推的潜力和可靠性。最后，应用ZqAOP模型预测了8种化合物在具有AR活性的环境浓度和全球地表水样品中的潜在生殖损伤。这项研究为筛选和评估大量具有潜在内分泌干扰作用的化学物质提供了新的见解。

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引用次数: 0

The mechanism of selenomethionine alleviating fluoride-induced brain injury in carp by regulating mitochondrial dynamics, oxidative stress and neuroinflammation: Integrated analysis of transcriptomics and metabolomics 硒代蛋氨酸通过调节线粒体动力学、氧化应激和神经炎症减轻氟化鲤脑损伤的机制：转录组学和代谢组学的综合分析

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-16 DOI: 10.1016/j.aquatox.2026.107722

Wenjie Wang , Mengyan Xu , Yongju Luo , Wenxuan Wang , Lijing He , Qianxi Han , Bo Liu , Lijuan Li , Jianjie Chen , Jinling Cao

This study was designed to clarify the protective effect of selenomethionine (Se-Met) and the underlying molecular mechanism on brain injury induced by fluoride (F) in carp. Using carp as the model organism, F injury model and Se-Met intervention models were established. Physiological and biochemical indicators (antioxidant enzyme activities, ATP synthesis efficiency, etc.), molecular biological analysis (apoptosis- and inflammation- related gene expression) and as well as transcriptomics and metabolomics technology were integrated to comprehensively investigate the pathological process of F-induced brain injury and the protective mechanism of Se-Met. The results showed that F significantly activated the NF-κB signaling pathway, induced mitochondrial dynamics imbalance (DRP1 up-regulation/MFN1 down-regulation), oxidative stress (decreased activities of GSH, SOD; increased MDA) and neuroinflammation (up-regulation of IL-1β, TNF-α; down-regulation of IL-10), eventually leading to neuronal apoptosis and behavioral disorders. Se-Met intervention effectively reversed these toxic effects through multiple pathways, specifically by restoring antioxidant enzyme activities (T-SOD, CAT, GSH-Px, GSH); Se-Met up-regulated the expression of anti-apoptotic gene Bcl-2 and down-regulated the expression of pro-apoptotic genes Bax and caspase-3 to inhibit neuronal apoptosis; Se-Met inhibited NLRP3 inflammasome activation and reduced the release of pro-inflammatory cytokines (TNF-α, IL-1β, IL-1α, IL-10, TGF-β); Se-Met balanced mitochondrial dynamics protein (DRP1/MFN1) expression and restored mitochondrial function (ATP and membrane potential recovery); Se-Met reversed abnormal expression of neuropeptides (GnRH-II, vip, PDYN). The integrated analysis of metabolomics and transcriptomics further revealed that Se-Met exerted neuroprotective effects by regulating calcium signaling pathway, arachidonic acid metabolism and neuroactive ligand-receptor interaction pathway. However, long-term exposure to Se-Met alone may also cause mild toxicity, indicating that its application requires strict control of dosage and exposure duration. This study elucidates the molecular mechanisms by which Se-Met mitigates F neurotoxicity and provides a theoretical basis for aquatic ecological risk assessment and selenium nutrition intervention strategies.

本研究旨在阐明硒代蛋氨酸（Se-Met）对氟化物(F)致鲤鱼脑损伤的保护作用及其分子机制。以鲤鱼为模型生物，建立了F损伤模型和Se-Met干预模型。结合生理生化指标（抗氧化酶活性、ATP合成效率等）、分子生物学分析（凋亡和炎症相关基因表达）以及转录组学和代谢组学技术，全面探讨f诱导脑损伤的病理过程和Se-Met的保护机制。结果表明，F显著激活NF-κB信号通路，诱导线粒体动力学失衡（DRP1上调/MFN1下调）、氧化应激（GSH、SOD活性降低，MDA升高）和神经炎症（IL-1β、TNF-α上调，IL-10下调），最终导致神经元凋亡和行为障碍。硒- met干预通过多种途径有效逆转这些毒性作用，特别是通过恢复抗氧化酶活性（T-SOD、CAT、GSH- px、GSH）；Se-Met上调抗凋亡基因Bcl-2的表达，下调促凋亡基因Bax和caspase-3的表达，抑制神经元凋亡；硒met抑制NLRP3炎性体活化，减少促炎因子（TNF-α、IL-1β、IL-1α、IL-10、TGF-β）的释放；硒- met平衡线粒体动力学蛋白（DRP1/MFN1）表达，恢复线粒体功能（ATP和膜电位恢复）；Se-Met可逆转神经肽（GnRH-II、vip、PDYN）的异常表达。代谢组学和转录组学综合分析进一步揭示Se-Met通过调节钙信号通路、花生四烯酸代谢和神经活性配体-受体相互作用通路发挥神经保护作用。然而，长期单独暴露于硒- met也可能引起轻度毒性，表明其应用需要严格控制剂量和暴露时间。本研究阐明了硒met减轻F神经毒性的分子机制，为水生生态风险评价和硒营养干预策略提供理论依据。

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引用次数: 0

Mechanistic insights into the effects of Fluoxetine in Mytilus galloprovincialis using in vivo and in vitro approaches 氟西汀对紫贻贝体内和体外作用的机理研究

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107717

Teresa Balbi , Paola Valbonesi , Marilin Profita , Caterina Ciacci , Elena Fabbri , Laura Canesi

Antidepressants such as selective serotonin reuptake inhibitors (SSRIs) are pseudo-persistent contaminants in aquatic ecosystems. While their effects and molecular targets are relatively well understood in vertebrates, less information is available in aquatic invertebrates. In this work, the effects and mechanisms of action of Fluoxetine (FLX), a widely prescribed SSRI, were investigated in the model marine bivalve Mytilus galloprovincialis. In vitro exposure of isolated hemocytes to FLX (0.03–0.3–3 µg/mL) induced significant alterations in immune and lysosomal parameters. In mussels exposed to FLX or its active metabolite norfluoxetine NFL (0.5–5–10–50 ng/L, 7 days), a concentration dependent accumulation of either compound was observed in the digestive gland. NFL was also detected in FLX-exposed mussels, indicating biotransformation. FLX and NFL affected transcription of monoamine receptors, and of lysosomal, autophagy, and ceramide related genes, with a distinct pattern for each compound, with FLX mainly inducing downregulation of gene expression. The results demonstrate that in mussels both FLX and NFL act through multiple molecular pathways, pointing at the lysosomal system as a main target for both compounds. These data provide novel mechanistic insights into antidepressant toxicity in a non-target marine invertebrate and contribute to draw Adverse Outcome Pathways for SSRIs in bivalves, that represent foundation species in coastal environments.

抗抑郁药如选择性血清素再摄取抑制剂（SSRIs）是水生生态系统中的伪持久性污染物。虽然它们在脊椎动物中的作用和分子靶点相对较好地了解，但在水生无脊椎动物中可获得的信息较少。在这项工作中，氟西汀（FLX）是一种广泛使用的SSRI，研究了模型海洋双壳贝贻贝（Mytilus galloprovincialis）的作用和机制。将分离的血细胞体外暴露于FLX（0.03-0.3-3µg/mL）可引起免疫和溶酶体参数的显著改变。在暴露于FLX或其活性代谢物去氟西汀NFL （0.5-5-10-50 ng/L， 7天）的贻贝中，在消化腺中观察到两种化合物的浓度依赖性积累。在暴露于flx的贻贝中也检测到NFL，表明生物转化。FLX和NFL影响单胺受体、溶酶体、自噬和神经酰胺相关基因的转录，每种化合物具有不同的模式，FLX主要诱导基因表达下调。结果表明，在贻贝中，FLX和NFL都通过多种分子途径起作用，指出溶酶体系统是这两种化合物的主要靶点。这些数据为非靶海洋无脊椎动物的抗抑郁药物毒性提供了新的机制见解，并有助于绘制双壳类动物中SSRIs的不良后果通路，双壳类动物是沿海环境中的基础物种。

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引用次数: 0

Developmental and neurobehavioural toxicity of per- and polyfluoroalkyl substances in aquatic organisms: mechanistic insights from molecular disruption to behavioural dysfunction 水生生物中全氟烷基和多氟烷基物质的发育和神经行为毒性：从分子破坏到行为功能障碍的机制见解

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107719

Mohamed Hamed , Mohamed Abbas , A K M Munzurul Hasan , Shimaa M. Kteeba , Hesham Taher , Mohammed Abdel-Wahab , Rashad E.M. Said , Zeinab Bakr , Douglas P. Chivers , Jae-Seong Lee , Alaa El-Din H. Sayed

Per- and polyfluoroalkyl substances (PFAS) are persistent synthetic contaminants ubiquitously detected in aquatic ecosystems, raising growing concern over their ecological and toxicological impacts. Increasing evidence demonstrates that PFAS pose significant risks to aquatic organisms, particularly during early developmental stages, by disrupting development and neurobehavioural function. This review synthesizes current knowledge on PFAS-induced developmental and neurobehavioural toxicity in aquatic organisms, integrating molecular, physiological, and behavioural evidence. Developmental exposure to legacy PFAS (e.g., PFOS, PFOA) and emerging alternatives (e.g., GenX, ADONA, HFPO-DA) are associated with embryotoxicity, pericardial edema, craniofacial abnormalities, impaired swim bladder inflation, metabolic disruption, and reduced growth and survival. Neurobehavioural effects include altered locomotion, anxiety-like behaviour, sensory processing, predator–prey interactions, and reproductive behaviours, with some effects persisting across life stages and generations. These outcomes are mechanistically linked to oxidative stress, mitochondrial dysfunction, endocrine disruption particularly perturbation of the hypothalamic-pituitary -thyroid axis neurotransmitter dysregulation, and transcriptomic, epigenetic, and non-coding RNA reprogramming. Emerging evidence also implicates microglial and neuroimmune dysfunction in PFAS-induced neurotoxicity. Notably, many PFAS alternatives exhibit toxicological profiles comparable to legacy compounds, challenging assumptions of safer substitution. This review highlights the need to integrate neurobehavioural and omics-based endpoints into PFAS risk assessment and regulatory frameworks to better protect aquatic ecosystems.

全氟和多氟烷基物质是水生生态系统中普遍存在的持久性合成污染物，其生态和毒理学影响日益引起人们的关注。越来越多的证据表明，PFAS通过破坏发育和神经行为功能，对水生生物构成重大风险，特别是在早期发育阶段。这篇综述综合了目前关于pfas诱导水生生物发育和神经行为毒性的知识，整合了分子、生理和行为证据。在发育过程中暴露于遗留的PFAS（如PFOS、PFOA）和新出现的替代品（如GenX、ADONA、HFPO-DA）与胚胎毒性、心包水肿、颅面异常、膀胱肿胀受损、代谢紊乱、生长和存活率降低有关。神经行为影响包括改变运动、焦虑样行为、感觉处理、捕食者-猎物相互作用和生殖行为，有些影响会持续到生命的各个阶段和几代人。这些结果与氧化应激、线粒体功能障碍、内分泌干扰（特别是下丘脑-垂体-甲状腺轴神经递质失调）以及转录组学、表观遗传学和非编码RNA重编程有关。新出现的证据也暗示pfas诱导的神经毒性中存在小胶质细胞和神经免疫功能障碍。值得注意的是，许多PFAS替代品显示出与传统化合物相当的毒理学特征，挑战了更安全替代品的假设。这篇综述强调了将神经行为学和组学端点纳入PFAS风险评估和监管框架以更好地保护水生生态系统的必要性。

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引用次数: 0

Damages of aged-PVC microplastics exceed the enhanced resistance of chlorella pyrenoidosa induced by phosphorus limitation 老化聚氯乙烯微塑料的损伤超过限磷诱导的蛋白核小球藻增强抗性

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107715

Xiao Tan , Lv Wang , Chao Xing , Zhipeng Duan , Jia Liang , Rui Zhu , Jiang Huang , Shichao Chen , Yijia Wei

Phytoplankton could adapt to multiple natural stresses. However, few have explicitly explored their complex adaptation patterns to emerging chemical contaminants (ECCs), such as microplastics (MPs), under nutrient-limited conditions. Here, we experimentally investigated the patterns of Chlorella pyrenoidosa responding to aged polyvinyl chloride (aged-PVC) and their leachate with a series of concentrations (0–20 mg/L) under phosphorus (P) repletion conditions (7.14 mg P/L) and P limitation conditions (0.1 mg P/L). Results revealed that C. pyrenoidosa displayed comparable patterns in response to aged-PVC treatments, regardless of P availability, showing significant decreases in growth rate but increases in photosynthetic efficiency (1.7%-39.6%), and the early-stage growth stimulation (day 2–14) may be attributed to the leachate. Moreover, cells of C. pyrenoidosa under both P availability elevated their cellular P quotas at the stationary phase under the low aged-PVC concentration (0.1 mg/L). These are significantly inconsistent with the responses of phytoplankton to multiple natural stresses, where phytoplankton typically enhance their resistance to environmental stresses under nutrient-limited conditions. This can be attributed to the stronger chemical toxicity of aged-PVC on enzymes and physical damage to the cell membrane. These findings suggest that cellular adaptive mechanisms of phytoplankton to natural stresses may fail against chemical contaminants, therefore providing crucial insights into the threshold dynamics of ecosystem resilience.

浮游植物可以适应多种自然压力。然而，很少有人明确探索它们在营养有限的条件下对新出现的化学污染物（ECCs）（如微塑料（MPs））的复杂适应模式。本研究在磷(P)补充条件（7.14 mg P/L）和磷限制条件（0.1 mg P/L）下，对不同浓度（0 ~ 20 mg/L）的陈化聚氯乙烯及其渗滤液的响应规律进行了实验研究。结果表明，无论磷有效性如何，pyrenoidosa对老化pvc处理的响应模式相似，其生长速率显著降低，但光合效率显著提高（1.7% ~ 39.6%），早期生长刺激（2 ~ 14天）可能归因于渗滤液。此外，两种磷效度下，在低老化pvc浓度（0.1 mg/L）条件下，固定期的细胞磷配额均有所增加。这与浮游植物对多种自然胁迫的反应明显不一致，浮游植物通常在营养有限的条件下增强对环境胁迫的抵抗力。这可归因于老化pvc对酶的化学毒性和对细胞膜的物理损伤。这些发现表明，浮游植物对自然胁迫的细胞适应机制可能无法抵抗化学污染物，因此为生态系统恢复力的阈值动力学提供了重要的见解。

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引用次数: 0

Tetracycline toxicity in fish: Recent insights into physiological and health implications 四环素对鱼类的毒性：对生理和健康影响的最新见解

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107716

Dip Mukherjee , Purandar Sarkar , Surajit Ghosh , Ahamadul Hoque Mandal , Sutapa Sanyal , Saurabh Chakraborti , Nimai Chandra Saha , Paolo Pastorino , Shubhajit Saha

Tetracyclines (TCs), a class of broad-spectrum antibiotics widely used in aquaculture, pose significant risks to fish health because of their environmental persistence and tendency to bioaccumulate in aquatic systems. This review synthesizes the toxic effects of TCs across key fish species (e.g., Danio rerio, Cyprinus carpio). TCs compromises respiratory efficiency via gill lamellar fusion, induces intestinal atrophy and inflammation, and alters hematological profiles, impairing oxygen transport and immune function. Biochemically, it disrupts antioxidant defenses, leading to pervasive oxidative stress. At the molecular level, TCs induces genotoxicity. Early life stages exhibit developmental toxicity (e.g., spinal deformities). Immunologically, TCs suppresses phagocytic activity and cytokine release, heightening infection susceptibility. Furthermore, it interferes with bone mineralization and causes muscle degeneration. Overall, TCs contamination represents a substantial threat to fish health and aquatic food webs, necessitating stricter regulations and improved removal strategies.

四环素（TCs）是一类广泛应用于水产养殖的广谱抗生素，由于其环境持久性和在水生系统中的生物积累倾向，对鱼类健康构成重大风险。本综述综合了TCs对主要鱼类（如达尼罗鱼、鲤鱼）的毒性作用。TCs通过鳃板融合降低呼吸效率，诱导肠道萎缩和炎症，改变血液学特征，损害氧气运输和免疫功能。从生物化学角度来看，它会破坏抗氧化防御，导致普遍的氧化应激。在分子水平上，TCs诱导遗传毒性。生命早期表现出发育毒性（如脊柱畸形）。免疫上，TCs抑制吞噬活性和细胞因子释放，增加感染易感性。此外，它会干扰骨矿化并导致肌肉退化。总体而言，TCs污染对鱼类健康和水生食物网构成重大威胁，需要更严格的法规和改进的清除策略。

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引用次数: 0

Ecotoxicological effects of differently charged polystyrene nanoparticles on sperm motility and early embryo mortality in European whitefish 不同电荷聚苯乙烯纳米颗粒对欧洲白鱼精子活力和早期胚胎死亡率的生态毒理学影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107721

Dissanayakage Dilshan Sampath Dissanayaka , Fazel Abdolahpur Monikh , Jukka Kekäläinen , Hannu Huuskonen , Lan Dupuis , Matti Janhunen , Jussi VK Kukkonen , Wujun Xu , Vesa-Pekka Lehto , Raine Kortet

Polystyrene, a commonly used plastic, can have significant impacts on natural ecosystems where it accumulates via various ways. While the ecotoxicological effects of nanoparticles on freshwater fish are increasingly understood, charge-dependent toxicity has remained virtually unstudied. Here, we explored the effects of positively and negatively charged polystyrene nanoparticles (250 nm) on sperm quality and early embryo mortality in European whitefish, Coregonus lavaretus. Gametes were exposed to both positively and negatively charged nanoparticles during sperm activation and for three minutes after fertilization (medium concentrations for sperm motility: 0.1, 1 and 10 mg/L, and for embryo mortality, 0.1 and 1 mg/L). The motility parameters (curvilinear velocity, straight line velocity, straightness, linearity) and longevity of activated sperm were analysed by computer-assisted sperm analysis. The early mortality was studied in two family-based settings: 1) a full-factorial mating design of five males × three females, where embryos were incubated in a stable environment, taking into account parental effects in addition to possible PS-NPs induced toxicity, and 2) a single-pair mating design with five full-sib families incubated in a stressful environment (variable turbulence and oxygen conditions). There were no significant differences between the treatments in any of the sperm motility parameters or sperm longevity. In both incubation settings, the highest early embryo mortality was recorded in a group exposed to positively charged nanoparticles. However, the difference was statistically significant only in the stressful environment, where concentration-dependent toxicity of nanoparticles was observed. The present study suggests possible concentration-dependent toxicity effects of PS-NPs on early embryo mortality in whitefish. This study also emphasizes the significance of different incubation conditions, as possible ecotoxicological effects may sometimes be observed only in a stressful environment.

聚苯乙烯是一种常用的塑料，它会通过各种方式积累，对自然生态系统产生重大影响。虽然人们越来越了解纳米颗粒对淡水鱼的生态毒理学影响，但电荷依赖性毒性实际上尚未得到研究。本研究探讨了带正电荷和负电荷的聚苯乙烯纳米颗粒（250 nm）对欧洲白鱼（Coregonus lavaretus）精子质量和早期胚胎死亡率的影响。在精子激活期间和受精后三分钟，配子暴露于带正电荷和负电荷的纳米颗粒中（精子活力的中等浓度：0.1、1和10mg /L，胚胎死亡率的中等浓度：0.1和1mg /L）。通过计算机辅助精子分析，分析了激活精子的运动参数（曲线速度、直线速度、直线度、线性度）和寿命。在两种基于家庭的环境中研究了早期死亡率：1)全因子交配设计，5公3母，胚胎在稳定的环境中孵育，考虑到亲代效应以及可能的PS-NPs诱导毒性；2)单对交配设计，5个全同胞家庭在压力环境中孵育（可变湍流和氧气条件）。在精子活力参数或精子寿命方面，两种处理之间没有显著差异。在两种孵育环境中，暴露于带正电纳米粒子的一组早期胚胎死亡率最高。然而，这种差异仅在应激环境中具有统计学意义，在应激环境中观察到纳米颗粒的浓度依赖性毒性。本研究提示PS-NPs对白鱼早期胚胎死亡率可能存在浓度依赖性毒性作用。本研究还强调了不同孵育条件的重要性，因为可能的生态毒理学效应有时可能仅在应激环境中观察到。

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引用次数: 0

Species resistance and resilience to lithium and nickel stress: A comparative ecotoxicological assessment between mussels and clams 物种对锂和镍胁迫的抗性和恢复力：贻贝和蛤蜊的比较生态毒理学评估

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2026-01-15 DOI: 10.1016/j.aquatox.2026.107718

Gonçalo Meireles , Marta Cunha , Carla Leite , Amadeu M.V.M. Soares , Eduarda Pereira , Rosa Freitas

The rapid expansion of lithium-ion battery production has heightened the environmental relevance of lithium (Li) and nickel (Ni), sparking concerns about their potential release into aquatic ecosystems. This study investigated the ecotoxicological effects of Li and Ni, individually and in combination, as well as post-exposure recovery processes, in two ecologically and commercially important bivalves, the mussel Mytilus galloprovincialis and the clam Ruditapes philippinarum. Specimens were subjected to a 14-day exposure phase followed by a 14-day recovery period under clean water conditions. A comprehensive biomarker approach was applied to assess energy metabolism, antioxidant and detoxification responses, cellular damage, and neurotoxicity. Distinct species-specific response patterns were observed. Despite higher metal accumulation, mussels displayed relatively stable biochemical profiles during both exposure and recovery, indicating a higher capacity to withstand metal-induced stress. In contrast, clams showed pronounced activation of antioxidant and detoxification pathways, which was insufficient to fully restore biochemical parameters to control levels within the recovery period, resulting in persistent oxidative stress and energetic imbalance. Nickel induced stronger biological effects than Li, and combined exposure to both metals produced the most pronounced impacts, particularly in clams. Overall, the contrasting responses of mussels and clams reflect differences in exposure pathways and physiological strategies, highlighting their complementary value as bioindicators. These findings emphasise the importance of incorporating recovery dynamics into ecotoxicological assessments to better evaluate ecosystem vulnerability to emerging metal contaminants.

锂离子电池生产的快速扩张提高了锂（Li）和镍（Ni）的环境相关性，引发了人们对它们可能释放到水生生态系统中的担忧。本研究研究了Li和Ni对两种具有重要生态和商业价值的双壳类贻贝（Mytilus galloprovincialis）和菲律宾蛤（Ruditapes philippinarum）的生态毒理学效应，包括单独和联合作用，以及暴露后的恢复过程。标本经过14天的暴露期，然后在清水条件下进行14天的恢复期。采用综合生物标志物方法评估能量代谢、抗氧化和解毒反应、细胞损伤和神经毒性。观察到不同的物种特异性反应模式。尽管有较高的金属积累，但贻贝在暴露和恢复过程中都表现出相对稳定的生化特征，表明其承受金属诱导的应力的能力更高。相比之下，蛤蜊表现出明显的抗氧化和解毒途径的激活，但在恢复期生化参数不足以完全恢复到控制水平，导致持续的氧化应激和能量失衡。镍引起的生物效应比锂更强，同时暴露于这两种金属会产生最明显的影响，尤其是对蛤蜊。总的来说，贻贝和蛤蜊的不同反应反映了暴露途径和生理策略的差异，突出了它们作为生物指标的互补价值。这些发现强调了将恢复动力学纳入生态毒理学评估以更好地评估生态系统对新兴金属污染物的脆弱性的重要性。

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引用次数: 0