Aquatic Toxicology最新文献_第6页

Aroclor 1254 impairs visual and neurosensory signaling pathways independent of the aryl hydrocarbon receptor in larval zebrafish Aroclor 1254对斑马鱼幼虫的视觉和神经感觉信号通路的影响不依赖于芳烃受体

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-25 DOI: 10.1016/j.aquatox.2025.107695

Jason T. Magnuson , Corey S. Green , Jeffrey M. Morris , Rachel R. Leads , Claire R. Lay , Michel Gielazyn , Lisa Rosman , Aaron P. Roberts , Daniel Schlenk

Aroclor 1254 is a mixture of polychlorinated biphenyl (PCB) congeners that are present in aquatic systems originating from coolants, solvents, plasticizers, and shipbreaking. Aroclor 1254 can induce an eye tremor response in early life stage zebrafish (Danio rerio) following exposure to between 22.7 and 4140 µg/L Aroclor 1254 at 102 h post fertilization (hpf), as shown in our recent study. However, the underlying mechanism that induces this response, either acting through the aryl hydrocarbon receptor (AhR) or non-AhR pathways, is not well characterized. As such, transcriptomics, morphology, and targeted behavioral functions were assessed using previously exposed larvae. Transcriptomic profiling was conducted in 102 hpf larvae to elucidate what may be driving this tremor response using in silico bioinformatic software. Subsequent eye tremor responses, a visually mediated behavioral function assay, and eye morphology were assessed in 7 day post fertilization (dpf) larvae after embryonic treatment. Parkinson’s signaling pathway, opioid signaling pathway, and the negative regulation of general cell signaling and communication were the top enriched dose-dependent pathways predicted. Eye diameters were significantly decreased in 7 dpf larvae exposed to 173 µg/L Aroclor 1254, whereas the number of eye saccades, assessed with an optokinetic response assay, and eye tremor response behavior were increased following exposure to a concentration of ≥ 173 µg/L Aroclor 1254. This suggests that Aroclor 1254 acts through a cross-talk of visual and neuronal-based mechanisms, and that non-AhR pathways may be more important than AhR pathways in inducing visual effects, which may influence survivorship and recruitment in early life stage wild fish.

Aroclor 1254是多氯联苯（PCB）同系物的混合物，存在于水生系统中，源自冷却剂、溶剂、增塑剂和拆船。我们最近的研究表明，在受精后102小时，暴露于22.7至4140µg/L的Aroclor 1254中，Aroclor 1254可以诱导早期斑马鱼（Danio rerio）的眼震颤反应。然而，诱导这种反应的潜在机制，无论是通过芳烃受体（AhR）还是非AhR途径，都没有很好地表征。因此，使用先前暴露的幼虫评估转录组学，形态学和目标行为功能。利用硅生物信息学软件对102只hpf幼虫进行了转录组学分析，以阐明可能驱动这种震颤反应的原因。在胚胎处理后的受精后7天（dpf）幼虫中，对随后的眼震颤反应、视觉介导的行为功能测定和眼睛形态进行了评估。帕金森信号通路、阿片信号通路和一般细胞信号和通讯的负调控是预测的最富集的剂量依赖性通路。暴露于173µg/L Aroclor 1254的7只dpf幼虫的眼直径显著减小，而暴露于浓度≥173µg/L Aroclor 1254后，用光动力学反应试验评估的眼跳次数和眼震颤反应行为增加。这表明Aroclor 1254通过视觉和神经元机制的交叉作用起作用，非AhR通路在诱导视觉效果方面可能比AhR通路更重要，这可能影响野生鱼类生命早期的生存和招募。

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引用次数: 0

Chronic paternal or maternal exposure to florfenicol causes reproductive and developmental toxicity in marine medaka (Oryzias melastigma) 父亲或母亲接触氟苯尼考会导致海洋medaka （Oryzias melastigma）的生殖和发育毒性。

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-24 DOI: 10.1016/j.aquatox.2025.107692

Xingying Guo, Tan-Duc Nguyen, Qingshi Chen, Xiaomin Wu, Wenhua Liu, Zhen Wang

New pollutants impair the reproduction and development of offspring in marine fish after parental exposure. While florfenicol has been frequently detected in the environment and organisms, limited information is available on its effects on the endocrine system of marine fishes and their developmental defects in offspring. In this study, six-month-old marine medaka (Oryzias melastigma) were exposed to florfenicol at 0.1 and 89 μg/L (measured) for 28 days. A cross-mating experimental design was employed to generate offspring embryos from various cross-mates by combining and mating control and exposed O. melastigma (male or female), thereby differentiating maternal and paternal influences. The results showed that either paternal or maternal exposure to florfenicol could affect the embryonic development of offspring. Additionally, parental exposure to florfenicol resulted in lower hatching rates and increased mortality in F1 embryos, even when cultured in clean seawater. Furthermore, the embryos of each cross-breeding group displayed distinct expressions of apoptosis-related genes, indicating that florfenicol can induce varying degrees of apoptosis in embryos. Altered expressions of genes related to endocrine disruption in the gonads were also identified, and morphological changes were observed in ovarian and testicular tissue sections of adult fish. The florfenicol-treated group showed a decrease in mature oocytes in the ovaries and an increase in interstitial tissue in the tests. In general, chronic exposure to florfenicol caused energy metabolism disorders, altered the expression of endocrine disruption related genes, damaged gonadal tissues, and led to reproductive dysfunction in O. melastigma, thereby impairing the production and development of their offspring. Overall, the key findings of this study demonstrated that extended exposure of paternal or maternal exposure to florfenicol significantly induced reproductive toxicity in adult medaka, leading to developmental toxicity in their offspring.

新污染物在亲代接触后对海鱼后代的繁殖和发育造成损害。虽然氟苯尼考经常在环境和生物体中被发现，但关于其对海洋鱼类内分泌系统的影响及其后代发育缺陷的资料有限。本研究以6个月大的海鳉鱼（Oryzias melastigma）为研究对象，分别暴露于0.1和89 μg/L（测量值）的氟苯尼考28天。采用交叉交配实验设计，通过组合、交配控制和暴露（雄性或雌性），从不同的杂交伴侣产生后代胚胎，从而区分母系和父系的影响。结果表明，父亲或母亲接触氟苯尼考均可影响后代的胚胎发育。此外，父母接触氟苯尼考导致F1胚胎的孵化率降低和死亡率增加，即使在清洁海水中培养也是如此。此外，各杂交组胚胎的凋亡相关基因表达不同，表明氟苯尼考可诱导不同程度的胚胎凋亡。性腺中与内分泌干扰相关的基因表达也发生了改变，在成年鱼的卵巢和睾丸组织切片中观察到形态学变化。氟苯尼考组显示卵巢成熟卵母细胞减少，间质组织增加。总的来说，长期暴露于氟苯尼考可引起能量代谢紊乱，改变内分泌干扰相关基因的表达，损伤性腺组织，导致褐斑O.生殖功能障碍，从而影响其后代的生产和发育。总体而言，本研究的主要发现表明，父亲或母亲长时间暴露于氟苯尼考可显著诱导成年medaka的生殖毒性，从而导致其后代的发育毒性。

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引用次数: 0

Intestinal toxicity and microbiota dysbiosis in zebrafish exposed to spirotetramat 接触螺虫的斑马鱼的肠道毒性和微生物群失调

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-23 DOI: 10.1016/j.aquatox.2025.107693

Xunyue Liu , Junyao Ge , Haojie Zhu , Peng Liu , Huidi Zhang , Qiong Rao , Wenze He , Jianqiang Sun

Spirotetramat is a novel, tetrahydrofuran-based broad-spectrum insecticide. It is widely used in global agriculture due to its high insecticidal activity. However, its long-term and extensive use causes environmental accumulation, making its toxicity to non-target organisms a key issue in environmental risk assessments. Previous studies have confirmed that it causes oxidative damage in zebrafish embryos and gonads. The toxic effects and mechanisms of spirotetramat in the zebrafish intestine remain unclear, despite its importance in digestion and absorption, immune defense, and microbiota-host interactions. This study systematically investigated the toxic effects of spirotetramat on zebrafish intestine using multidimensional approaches, including histopathological observation, detection of oxidative stress-related gene expression, and analysis of intestinal microbiota. This study found that gene expression levels of sod, cat, and gpx increased in the 0.08 mg/L group but decreased in the 2 mg/L group, indicating that low concentrations activate the intestinal antioxidant system, while high concentrations cause significant overload. Histopathological analysis revealed intestinal damage, including villus fragmentation and epithelial detachment, in the 2 mg/L treatment group. The increased alkaline phosphatase activity in the 2 mg/L group may be associated with compensatory repair. Microbiota analysis revealed an elevated quantity and diversity of intestinal flora in the treatment groups, with altered abundance of specific bacteria in the 2 mg/L treatment group. Functional prediction of the microbiota suggested that spirotetramat exposure induced changes in metabolic functions. In conclusion, spirotetramat induced intestinal oxidative stress in zebrafish, leading to intestinal barrier damage and microbiota dysbiosis. This study provides a theoretical basis for the comprehensive assessment of spirotetramat's ecological risks.

螺虫是一种新型的基于四氢呋喃的广谱杀虫剂。由于其高杀虫活性，在全球农业中得到广泛应用。然而，由于其长期和广泛的使用会引起环境累积，使其对非目标生物的毒性成为环境风险评估的关键问题。先前的研究已经证实，它会导致斑马鱼胚胎和性腺的氧化损伤。尽管螺虫在消化吸收、免疫防御和微生物-宿主相互作用中具有重要作用，但其在斑马鱼肠道中的毒性作用和机制尚不清楚。本研究采用组织病理学观察、氧化应激相关基因表达检测、肠道菌群分析等多维方法，系统研究了螺虫碱对斑马鱼肠道的毒性作用。本研究发现，sod、cat、gpx基因表达水平在0.08 mg/L组升高，在2 mg/L组降低，说明低浓度激活肠道抗氧化系统，高浓度引起明显超载。组织病理学分析显示，2 mg/L治疗组出现肠损伤，包括绒毛碎裂和上皮脱离。2 mg/L组碱性磷酸酶活性升高可能与代偿性修复有关。微生物群分析显示，处理组肠道菌群的数量和多样性增加，特定细菌的丰度在2 mg/L处理组发生改变。微生物群的功能预测表明，螺虫暴露会引起代谢功能的变化。综上所述，螺虫可诱导斑马鱼肠道氧化应激，导致肠道屏障损伤和微生物群失调。本研究为螺虫生态风险的综合评价提供了理论依据。

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引用次数: 0

Nanoplastics intensify metal-induced impacts in freshwater ecosystems 纳米塑料加剧了金属对淡水生态系统的影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-21 DOI: 10.1016/j.aquatox.2025.107691

Juliana Barros , Santosh Kumar , Sarra Ben Tanfous , Manuel Graça , Sahadevan Seena

Nanoplastics (NPs) are an emerging concern in freshwater ecosystems due to their persistence and potential to interact with persistent pollutants, such as metals. These combined stressors threaten freshwater ecosystems functioning, where leaf litter decomposition, primarily driven by aquatic hyphomycetes, supports energy transfer to higher trophic levels. A microcosm experiment was conducted to assess the joint impacts of polystyrene NPs (bare and carboxylated) at environmentally relevant (0.25 and 2.5 µg L⁻¹) and elevated (25 and 250 µg L⁻¹) concentrations in reference and metal-polluted stream waters. Fungal biomass, aquatic hyphomycetes sporulation and community composition, microbial leaf litter decomposition, as well as invertebrate feeding behaviour were quantified. Carboxylated NPs showed greater aggregation and surface alterations than bare NPs. Fungal biomass and sporulation declined significantly at 2.5 µg L⁻¹, with carboxylated NPs exerting stronger effects. Non-metric multidimensional scaling (NMDS) revealed clear shifts in fungal community structure across two water types (reference and metal-polluted) and NP concentrations. Metal pollution alone reduced decomposition by 17 %, with reductions reaching up to 24 % when combined with NPs, particularly carboxylated NPs. Invertebrate feeding was reduced by 27 % under metal pollution alone, with maximum inhibition (47 %) observed at the highest NP concentration, although NP type did not significantly alter feeding rates. Together, these results demonstrate that NPs, especially carboxylated forms, exacerbate the ecological impacts of metal pollution, impairing microbial processes and detritivore feeding. These findings underscore the importance of considering NPs surface chemistry and multiple stressors interactions in ecological risk assessments of polluted freshwater systems.

纳米塑料（NPs）由于其持久性和与持久性污染物（如金属）相互作用的潜力而成为淡水生态系统中一个新兴的问题。这些综合的压力源威胁着淡水生态系统的功能，在淡水生态系统中，主要由水生菌丝菌驱动的凋落叶分解支持能量向更高营养水平的转移。我们进行了一个微观实验，以评估聚苯乙烯NPs（裸的和羧化的）在环境相关（0.25和2.5µg L -⁻¹）和在参考和金属污染的溪流中浓度升高（25和250µg L -⁻¹）时的联合影响。对真菌生物量、水生菌丝菌产孢量和群落组成、微生物凋落叶分解以及无脊椎动物摄食行为进行了量化。羧基化NPs比裸NPs表现出更大的聚集和表面变化。在2.5µg L - 1时，真菌生物量和产孢量明显下降，羧化NPs的作用更强。非度量多维尺度（NMDS）揭示了两种水类型（参考水和金属污染水）和NP浓度下真菌群落结构的明显变化。仅金属污染就使分解率降低了17%，当与净污染物（特别是羧化净污染物）结合使用时，分解率可达24%。在金属污染下，无脊椎动物的摄食减少了27%，在最高NP浓度下观察到最大的抑制（47%），尽管NP类型没有显著改变摄食率。总之，这些结果表明，NPs，特别是羧基化形式，加剧了金属污染的生态影响，损害了微生物过程和营养物质的摄食。这些发现强调了在污染淡水系统生态风险评估中考虑NPs表面化学和多种应激源相互作用的重要性。

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引用次数: 0

Transient cognitive and affective impairments following short-term aluminum exposure in adult zebrafish 成年斑马鱼短期铝暴露后的短暂认知和情感障碍

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-21 DOI: 10.1016/j.aquatox.2025.107690

Gustavo Axel Elizalde-Velázquez , Selene Elizabeth Herrera-Vázquez , Niki Tagkalidou , Melissa Faria , Irene Romero-Alfano , Eva Prats , Ariadna Verdaguer , Cristian Gómez-Canela , Leobardo Manuel Gómez-Oliván , Demetrio Raldúa

Aluminum (Al) is a widespread aquatic neurotoxic pollutant, yet its brain accumulation is seldom quantified in fish neurotoxicity studies. Here, we investigated the neurobehavioral and molecular consequences of acute waterborne Al exposure in adult zebrafish (n = 227). Fish were exposed for 96 h to 50 mg/L AlCl₃ at pH 5.0 and then transferred to clean water for a 7-day depuration period. Despite the absence of statistically significant Al accumulation in either brain or carcass, exposed animals exhibited consistent neurobehavioral impairments, including reduced non-associative learning (short-term habituation of the acoustic startle response), anxiety- and depression-like phenotypes (positive geotaxis and negative scototaxis), and increased aggressive-like behavior. All behavioral alterations were fully reversed after depuration, indicating a transient effect. Brain oxidative stress markers (catalase, superoxide dismutase, lipid peroxidation), neurotransmitter levels, and acetylcholinesterase activity remained largely unchanged, arguing against a direct, generalized disruption of brain biochemistry. In contrast, brain expression of appa, gfap, and cat was significantly upregulated immediately after exposure and returned to control levels after depuration, suggesting an early but reversible stress and glial response. Overall, these findings show that short-term acidic Al exposure can induce reversible cognitive and affective disturbances in zebrafish in the absence of detectable brain accumulation, highlighting the importance of transient molecular stress pathways in acute aluminum neurotoxicity.

铝（Al）是一种广泛存在的水生神经毒性污染物，但在鱼类神经毒性研究中很少量化其脑蓄积。在这里，我们研究了成年斑马鱼急性水性铝暴露的神经行为和分子后果（n = 227）。鱼在pH 5.0的50 mg/L AlCl₃中暴露96小时，然后转移到清洁水中进行7天的净化期。尽管在大脑或尸体中没有统计上显著的Al积累，但暴露的动物表现出一致的神经行为障碍，包括非联想学习减少（声惊吓反应的短期习惯化），焦虑和抑郁样表型（正地向性和负地向性），以及攻击性行为增加。净化后，所有的行为改变都完全逆转，表明这种影响是短暂的。大脑氧化应激标志物（过氧化氢酶、超氧化物歧化酶、脂质过氧化）、神经递质水平和乙酰胆碱酯酶活性基本保持不变，这表明大脑生物化学不存在直接、广泛的破坏。相比之下，appa、gfap和cat的脑表达在暴露后立即显著上调，并在净化后恢复到控制水平，表明早期但可逆的应激和神经胶质反应。总的来说，这些研究结果表明，短期酸性铝暴露可以在没有可检测到的大脑积累的情况下诱导斑马鱼可逆的认知和情感障碍，突出了急性铝神经毒性中瞬时分子应激途径的重要性。

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引用次数: 0

Corrigendum to “A whale in a well: Co-exposure of a persistent organic pollutant mixture and cetacean morbillivirus on killer whale (Orcinus orca) primary fibroblasts” [Aquatic Toxicology, 291, (2026) 107671] “井中的鲸鱼：虎鲸（Orcinus orca）原代成纤维细胞上持久性有机污染物混合物和鲸类病毒的共同暴露”的勘误表[水生毒理学，291,(2026)107671]

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-19 DOI: 10.1016/j.aquatox.2025.107684

Helena Costa , Maud Van Essche , Juliane A. Riedel , Akash Gupta , Audun H. Rikardsen , Anders Goksøyr , Pierre Blévin , Mikael Harju , Laura Pirard , Susan Bengtson Nash , Sofie Søderstrøm , Courtney A. Waugh

引用次数: 0

Copper- and zinc-based metal-organic frameworks (MOFs) as sustainable algicides: Effective inhibition of Karenia selliformis blooms and toxin production with ecotoxicological assessment on Artemia salina 铜和锌基金属有机框架（mof）作为可持续的杀藻剂：有效抑制selliformia Karenia华和毒素产生及对Artemia salina的生态毒理学评价

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-18 DOI: 10.1016/j.aquatox.2025.107689

Mohammad Sadiq Nikzad , Jiangbing Qiu , Guixiang Wang , Natnael Sisay Demo , Jinglin Liu , Aifeng Li

Marine dinoflagellate Karenia selliformis, as a notorious fish-killing microalga, poses a serious threat to aquatic ecosystems and aquaculture due to its production of neurotoxins such as gymnodimines (GYMs). To look for some sustainable alternatives of traditional algicides, metal organic frameworks (MOFs) have attracted attention of researchers. The effectiveness and ecotoxicological impacts of Cu-MOF and Zn-MOF on K. selliformis and the brine shrimp (Artemia salina) were investigated in this study. Both MOFs inhibited the growth of K. selliformis in a dose- and time-dependent pattern, with Cu-MOF being more effective at lower concentrations (89% inhibition at 1.0 mg L⁻¹ after 96 h). The MOFs decreased the photosynthetic parameter F_v/F_m, enhanced content of chlorophyll a and induced oxidative stress in the experimental microalga. Transmembrane internalization of MOFs was validated using a confocal microscopy, and a dose-dependent increase in intracellular Cu²⁺ and Zn²⁺ accumulation was verified. Exposure to MOFs reduced contents of both intracellular and extracellular gymnodimine A (GYM-A). Ecotoxicological evaluation on A. salina revealed concentration-dependent mortality, with Cu-MOF being more toxic (83.3% mortality at 5 mg L⁻¹ after 72 h) than Zn-MOF (11.7% at the same concentration and time). While higher MOF concentrations led to increased larval mortality and oxidative stress, concentrations ≤ 1.0 mg L⁻¹ for Cu-MOF and ≤ 10 mg L⁻¹ for Zn-MOF were within safe thresholds for A. salina. These findings highlight the potential of MOFs as agents for mitigation of harmful algal blooms (HABs) but underscore the necessity of careful dosage optimization to balance efficacy with environmental safety.

海洋甲藻（Karenia selliformis）是一种臭名昭著的杀鱼微藻，由于其产生的神经毒素如裸子二胺（gymnodinines, gym）对水生生态系统和水产养殖造成严重威胁。为了寻找一些可持续的传统杀藻剂替代品，金属有机框架（MOFs）引起了研究人员的关注。本文研究了Cu-MOF和Zn-MOF对沙棘海蛾和盐虾的药效及生态毒理学影响。两种mof均呈剂量和时间依赖性，其中Cu-MOF在较低浓度下更有效（1.0 mg L−1,96 h后抑制率为89%）。MOFs降低了实验微藻的光合参数Fv/Fm，提高了叶绿素a含量，诱导了氧化应激。使用共聚焦显微镜验证了mof的跨膜内化，并证实了细胞内Cu2+和Zn2+积累的剂量依赖性增加。暴露于MOFs降低了细胞内和细胞外裸子二胺A （gyma）的含量。对盐藻的生态毒理学评价显示出浓度依赖性死亡率，Cu-MOF的毒性大于Zn-MOF(在相同浓度和时间下，相同浓度和时间下，Cu-MOF的死亡率为83.3%，高于Zn-MOF（11.7%）。虽然较高的MOF浓度会增加幼虫的死亡率和氧化应激，但Cu-MOF浓度≤1.0 mg L−1和Zn-MOF浓度≤10 mg L−1都在盐芽孢杆菌的安全阈值范围内。这些发现强调了MOFs作为减缓有害藻华（HABs）药剂的潜力，但也强调了谨慎优化剂量以平衡效果与环境安全的必要性。

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引用次数: 0

Internal handling, shell accumulation and effects of cobalt on the gastropod Ampullaceana balthica 内处理、壳积累及钴对腹足动物壶足的影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-17 DOI: 10.1016/j.aquatox.2025.107688

Maëva Marimoutou , Gilles Bareille , Mathieu Milhe-Poutingon , Clémentine Gelber , Patrick Baldoni-Andrey , Nicholas Bagger Gurieff , Hélène Tabouret , Sandra Mounicou , Séverine Le Faucheur

Cobalt (Co) is central to the development of new low-carbon energies, becoming an element of emerging concern for the environment. Gastropods are widely distributed in aquatic ecosystems and represent the largest group of the phylum Mollusca. However very few studies have focused on Co impacts on gastropods. We thus aimed to examine the internal handling of Co in gastropods and its excretion into the shell in order to better understand its toxic actions and how these organisms cope with Co stress. To that end, Ampullaceana balthica was exposed to increasing Co concentrations (6, 30 and 60 μg·L⁻¹) using outdoor flow-through mesocosms for 28 days in June and October. They were analyzed for their growth, reproduction and Co accumulation in the soft bodies and the outer shell part every week. Cobalt distribution in soft tissues and its subcellular distribution were further examined.

Cobalt induced a decrease in the egg mass number and a delay in their hatching. Its accumulation in the soft tissue and the outer shell exhibited distinct seasonal patterns. Linear relationships with the ambient Co concentrations were only evident in the soft tissues in June and in the shell in October. For both periods, Co was mainly distributed in the hepatopancreas and the mantle, particularly in their mitochondria and granules fractions. We thus discussed the possible pathways of Co from the soft tissues to the shell involving granules. The present results demonstrate that using shells as bioindicators of metal exposure has certain limitations, depending on the organism life cycle.

钴（Co）是新型低碳能源发展的核心，成为人们日益关注的环境问题之一。腹足类动物广泛分布于水生生态系统中，是软体动物门中最大的一类。然而，很少有研究关注Co对腹足动物的影响。因此，我们旨在研究Co在腹足类动物中的内部处理及其排泄到壳中的情况，以便更好地了解其毒性作用以及这些生物如何应对Co应激。为此，在6月和10月，利用室外流动中生态系统对壶藻进行了28天的Co浓度升高处理（分别为6、30和60 μg·L−1）。每周对它们在软体和外壳部分的生长、繁殖和Co积累情况进行分析。进一步观察钴在软组织中的分布及其亚细胞分布。钴导致鸡蛋数量减少和孵化延迟。其在软组织和外壳中的积累表现出明显的季节性规律。与环境Co浓度的线性关系仅在6月的软组织和10月的壳中存在。在这两个时期，Co主要分布在肝胰脏和套膜中，尤其是在它们的线粒体和颗粒部分。因此，我们讨论了Co从软组织到颗粒壳的可能途径。目前的结果表明，利用贝壳作为金属暴露的生物指标有一定的局限性，这取决于生物体的生命周期。

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引用次数: 0

Molecular interactions and biomarker responses to pharmaceutical contaminants in aquatic species 水生物种对药物污染物的分子相互作用和生物标志物反应

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-16 DOI: 10.1016/j.aquatox.2025.107687

Dibakar Roy , Mokhtar Rejili , Wasim Ghafoor , Iqra Sarwar , Devendra Pratap Rao , Jayanthi Barasarath , Zamira Atamuratova , Urazbaeva Yulduz

Pharmaceutical residues are increasingly detected in freshwater systems, yet mechanistic links between sub-lethal exposure and organ-level injury in fish remain limited. This study assessed 21-day effects of carbamazepine (CBZ), diclofenac (DCF) and fluoxetine (FLX) on Labeo rohita using integrated hepatic and renal biomarker panels supported by molecular docking. Fish were exposed to sub-lethal concentrations under semi-static conditions, and responses were quantified using standard biochemical assays. Hepatic markers included cytochrome P4501A activity (CYP1A/EROD), uridine diphosphate-glucuronosyltransferase (UGT), glutathione S-transferase (GST), antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)], redox balance [reduced/oxidized glutathione ratio (GSH/GSSG)] and oxidative damage indicators such as malondialdehyde (MDA). Renal endpoints comprised organic anion transporters (OAT1/OAT3), cyclooxygenase-2 (COX-2), caspase-3, kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), N-acetyl-β-d-glucosaminidase (NAG), creatinine and urea. Homology models of CYP1A, COX-2, serotonin transporter (SERT), heat-shock protein 70 (HSP70) and vitellogenin (Vtg) were validated using QMEAN and docked with CB-Dock2. Responses ranked DCF > CBZ > FLX. Diclofenac caused the strongest biotransformation induction, antioxidant suppression, oxidative damage and renal injury, whereas CBZ induced moderate health effects and FLX produced mild changes. Findings highlight biomarker-anchored evidence of pharmaceutical risk to carp health and offer practical indicators for freshwater monitoring.

在淡水系统中越来越多地检测到药物残留，但亚致死暴露与鱼类器官水平损伤之间的机制联系仍然有限。本研究利用分子对接支持的综合肝脏和肾脏生物标志物面板，评估了卡马西平（CBZ）、双氯芬酸（DCF）和氟西汀（FLX）对罗希塔Labeo rohita 21天的影响。鱼在半静态条件下暴露于亚致死浓度，并使用标准生化分析对反应进行量化。肝脏标志物包括细胞色素P4501A活性（CYP1A/EROD）、尿苷二磷酸-葡萄糖醛基转移酶（UGT）、谷胱甘肽s -转移酶（GST）、抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）]、氧化还原平衡[还原/氧化谷胱甘肽比值（GSH/GSSG）]和氧化损伤指标如丙二醛（MDA）。肾终点包括有机阴离子转运蛋白（OAT1/OAT3）、环氧化酶-2 （COX-2）、caspase-3、肾损伤分子-1 （KIM-1）、中性粒细胞明胶酶相关脂钙蛋白（NGAL）、n -乙酰-β-d-氨基葡萄糖酶（NAG）、肌酐和尿素。CYP1A、COX-2、血清素转运体（SERT）、热休克蛋白70 （HSP70）和卵黄原蛋白（Vtg）的同源性模型使用QMEAN进行验证，并与CB-Dock2对接。回复排名为DCF >； CBZ >； FLX。双氯芬酸引起的生物转化诱导、抗氧化抑制、氧化损伤和肾损伤最强，而CBZ对健康的影响中等，FLX对健康的影响较轻。研究结果强调了生物标志物锚定的药物风险对鲤鱼健康的证据，并为淡水监测提供了实用指标。

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引用次数: 0

Non-additive effects of norethisterone and levonorgestrel mixtures on lipid metabolism at environmentally relevant concentrations 在环境相关浓度下，去甲睾酮和左炔诺孕酮混合物对脂代谢的非加性影响

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology

Pub Date : 2025-12-15 DOI: 10.1016/j.aquatox.2025.107686

Zeyan Zhang , Kejin Zhou , Yafang Chen , Kun Xie , Mo Zhao , Shimiao Zhao , Zhiqiang Xia , Wenjun Hong , Pan Ma , Xinyi Zhou

Norethindrone (NET) and levonorgestrel (LNG) are synthetic progestins frequently detected in aquatic environments, have unclear effects on lipid metabolic homeostasis during the early life stages of aquatic organisms. Although progestins commonly occur as mixtures, their combined impacts remain unclear. In this study, we investigated the individual and combined impacts of NET and LNG at environmentally relevant concentrations (2–200 ng/L) on lipid metabolism in zebrafish larvae. NET and LNG significantly disrupted early development in zebrafish. It also altered lipid profiles, as indicated by elevated triglyceride (TG) levels, reduced total cholesterol (TC), as well as alterations in key metabolic enzymes (FASN, LPL) and lipid-regulatory genes (pparγ, fasn, lpl, pparα). Co-exposure with LNG resulted in non-additive responses across multiple endpoints. Antagonistic interactions were predominant at medium and high concentrations, while occasional synergism was observed at low doses. These complex patterns were further supported by Bliss independence model analysis. Notably, combined exposure suppressed both lipid synthesis and degradation pathways more strongly than individual treatments, leading to lipid accumulation and altered energy regulation. This study advanced understanding of the ecological risks caused by progestins in aquatic environments and highlighted the necessity of mixture-based risk assessment of endocrine-disrupting compounds.

去甲thindrone （NET）和左炔诺孕酮（LNG）是在水生环境中经常检测到的合成孕激素，对水生生物生命早期脂质代谢稳态的影响尚不清楚。虽然孕激素通常以混合形式出现，但它们的综合影响尚不清楚。在这项研究中，我们研究了环境相关浓度（2-200 ng/L）的NET和LNG对斑马鱼幼鱼脂质代谢的单独和联合影响。NET和LNG显著破坏了斑马鱼的早期发育。它还改变了脂质谱，如甘油三酯（TG）水平升高，总胆固醇（TC）降低，以及关键代谢酶（FASN， LPL）和脂质调节基因（pparγ， FASN， LPL, pparα）的改变。与LNG共暴露导致跨多个端点的非加性反应。拮抗相互作用在中、高浓度下占优势，而在低剂量下偶有协同作用。Bliss独立模型分析进一步支持了这些复杂模式。值得注意的是，联合暴露比单独处理更强烈地抑制脂质合成和降解途径，导致脂质积累和能量调节改变。本研究提高了对水生环境中孕激素引起的生态风险的认识，并强调了对内分泌干扰化合物进行混合风险评估的必要性。

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