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Reproductive hormones in seminal plasma; it's effect on semen quality 精浆中的生殖激素;对精液质量的影响
Pub Date : 2008-10-22 DOI: 10.4038/CJMS.V48I2.122
S. Wijeratna, H. Seneviratne, WD Ratnasooriya
The aim of this study was to investigate the effect of reproductive hormones in seminal plasma on the quality of the spermatozoa in men seeking infertility treatment. Of the 383 volunteers recruited, 123 had normal sperm parameters and 260 had either a single or a combination of sperm abnormalities as per WHO criteria. Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL), Testosterone (T) and Estradiol (E2) levels in serum and semen of these men were determined using an immunometric chemiluminisance assay. Of the serum hormones, FSH, LH were significantly higher (p<0.001 for both) and T levels were significantly lower (p< 0.01) in men with abnormal semen compared with the men with normal semen parameters. Serum PRL and E2 in both the groups did not show any significant difference. With regard to semen hormones FSH, LH and T levels in men with normal and abnormal semen did not show any significant difference. In contrast, men with abnormal semen had significantly higher levels of semen PRL (p<0.001) and E2 (p<0.01) compared with those in men with normal semen. Moreover, semen PRL showed a significant negative correlation with sperm motility (r = -0.398; p<0.001), morphology (r = -0.32; p<0.01) and viability (r = -0.328; p<0.01). Further, a positive correlation between PRL and semen volume was observed in both semen normal and abnormal groups (r = 0.21; p<0.05). Semen E2 in the abnormal group also showed a significant negative relationship with sperm motility (r = ^).305; p < 0.01). In conclusion, in men with abnormal semen parameters both PRL and E2 in seminal plasma have a negative impact on sperm motility. It was also evident that semen PRL has a negative impact on sperm morphology and viability.
本研究的目的是探讨精液中生殖激素对寻求不育治疗的男性精子质量的影响。根据世卫组织的标准,在招募的383名志愿者中,123名精子参数正常,260名精子单一或多种异常。采用免疫化学发光法测定血清和精液中的促卵泡激素(FSH)、促黄体生成素(LH)、催乳素(PRL)、睾酮(T)和雌二醇(E2)水平。在血清激素方面,与精液参数正常的男性相比,精液异常的男性FSH、LH水平显著升高(p<0.001), T水平显著降低(p< 0.01)。两组血清PRL、E2水平差异无统计学意义。精液正常与异常男性的精液激素FSH、LH、T水平无显著差异。与精液正常的男性相比,精液异常男性的PRL (p<0.001)和E2 (p<0.01)水平显著高于正常男性。精液PRL与精子活力呈显著负相关(r = -0.398;P <0.001),形态学(r = -0.32;P <0.01)和存活率(r = -0.328;p < 0.01)。此外,在精液正常和异常组中,PRL与精液体积呈正相关(r = 0.21;p < 0.05)。异常组精液E2与精子活力呈显著负相关(r = ^)。305;P < 0.01)。综上所述,在精液参数异常的男性中,精浆中PRL和E2均对精子活力有负面影响。精液PRL对精子形态和活力有明显的负面影响。
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引用次数: 4
Nutrition Throughout the Lifecycle by Professor T.W.Wickramanayake 维克拉马纳亚克教授的《生命周期中的营养》
Pub Date : 2008-10-20 DOI: 10.4038/CJMS.V50I1.119
N. Warnasuriya
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引用次数: 2
A further case of Parastrongyliasis (= Angiostrongyliasis ) from the eye of a patient in Sri Lanka 斯里兰卡一名患者眼睛的另一例副圆线虫病(=血管圆线虫病)
Pub Date : 2008-10-20 DOI: 10.4038/CJMS.V50I1.118
Rl Ilhalamulla, S. Fernando, K. Weerasena, J. Cross, A. Dissanaike, C. Fonseka
Raw snails and slugs are rare ly, if ever, consumed in Sri Lanka. [f such mollusks are in fected with the 3rd stage larvae of the lungworm parasite Pnrnstrongyills (=AlIgiostrollgy/IIS), human in fect ion can occur and a number of such cases have been encountered in this country. We now report w hat we believe is the 5th such infection. All these cases have been from the eye, iI"Iciuding the anterior chmnber, the vitreous and the re ti na. In the present case the worm was in the vitreous.
在斯里兰卡,生蜗牛和蛞蝓很少被食用,如果有的话。[如果这些软体动物感染了肺寄生虫pnstrongyills (=AlIgiostrollgy/IIS)的第三期幼虫,就会发生人类感染,在这个国家已经遇到了一些这样的病例。]我们现在报告我们认为是第5例这样的感染。所有这些病例都来自眼睛,包括前房、玻璃体和视网膜。在这种情况下,蠕虫是在玻璃体。
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引用次数: 4
Effects of prenatal ginger rhizome extract treatment on pregnancy outcome and postnatal development of Sprague Dawley rats 产前姜提取物对大鼠妊娠结局及产后发育的影响
Pub Date : 2008-10-20 DOI: 10.4038/CJMS.V50I1.116
Dlo Dissabandara, Chandrasekara
The effect of prenatal exposure to ginger on pregnancy outcome and postnatal development of Sprague Dawley rats was studied. Pregnant rats were administered with dry powder extracts of ginger orally at doses of 500 or 1000 mg/kg/day from gestation day 5 to 15. The daily food and water intake was significantly reduced during the exposure period in both groups of ginger fed rats. The weight gain during the exposure period and the total weight gain in pregnancy were also significantly low in both groups of ginger fed rats compared to the control. There was a significant embryonic loss in ginger fed rats. The growth and physical maturation parameters of the offspring exposed to ginger were unaffected. The results of this study suggests that maternal administration of ginger during mid pregnancy results in reduced maternal weight gain and increased embryonic loss without affecting the postnatal growth and physical maturation of the surviving offspring.
研究了产前生姜暴露对大鼠妊娠结局及产后发育的影响。妊娠第5 ~ 15天口服生姜干粉提取物,剂量分别为500或1000 mg/kg/天。暴露期间,两组大鼠的日摄食量和饮水量均显著减少。与对照组相比,两组生姜喂养的大鼠在暴露期间的体重增加和怀孕期间的总体重增加也明显较低。饲喂生姜的大鼠有明显的胚胎丢失。接触生姜的后代的生长和生理成熟参数不受影响。本研究结果表明,妊娠中期母亲服用生姜可以减少母亲体重增加和增加胚胎损失,但不会影响幸存后代的出生后生长和身体成熟。
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引用次数: 1
Drug resistant enterococci: factors associated with gastrointestinal tract colonization 耐药肠球菌:与胃肠道定植相关的因素
Pub Date : 2008-10-20 DOI: 10.4038/CJMS.V50I1.117
S. Gunasekera, J. Perera
Resistant enterococci are recognized as important h ospital acquired pathogens. They are generally sensitive to ampicillin but intrinsicall y resis tant to cephalosporins and aminoglycosides and are known to acquire high le vel resistance to these drugs. High level resistance has serious implications for treatment of patients infected with lhese organisms and for infection control activities. Colonis ation of the gastro­ intestinal tract is a prerequi,site to infection. A number of fa ctors a re known to contribute to acquiring colonisa tion by resistant enterococci according to studies conducted · in the West. The present s tudy determines the prevalence of resis tant enterococci on admission in a cohort of hospitalised patients, and identifies SOll1e risk factors that are significantly associated with acquiring resistant enterococci during hospital stay . A prospective s tudy conducted over three months, on 125 patien ts in orthopaedic units and the burns unit of the Nationa l Hospital of Sri Lanka, to determine colonisation showed that 5 .6 % were colonised on admission and 16.8% acquired ampicillin res istant ente rococci (ARE) during hospitalisation. No vancomycin res is tant enterococci (V RE) were isola led. Risk faclors 1. Senior Registrar i11 Microbiology that were s ignificantly associated w ith colonisation were empiric use of antibiotics, trealment with ampiCillin, multiple use of antibiotics, prior hospitalisa t ion and presence of diabetes mellitus. These features will be helpful in identifying pOSS ible patients for screening for resistant enterococci, with a view to implementing more sh'ingent infection conlrol measures La prevent spread of these organisms,
耐药肠球菌被认为是重要的医院获得性病原体。它们通常对氨苄西林敏感,但对头孢菌素和氨基糖苷类具有内在抗性,并且已知对这些药物具有高度耐药性。高水平耐药性对感染这些生物的患者的治疗和感染控制活动具有严重影响。胃肠道的菌落是感染的先决条件和部位。根据在西方进行的研究,已知许多因素有助于获得耐药肠球菌的定植。本研究确定了一组住院患者入院时耐药肠球菌的患病率,并确定了与住院期间获得耐药肠球菌显著相关的soll11风险因素。一项针对斯里兰卡国立医院骨科和烧伤科125名患者进行的为期3个月的前瞻性研究显示,5.6%的患者在入院时被定植,16.8%的患者在住院期间获得了耐氨西林肠炎球菌(ARE)。未分离出万古霉素阳性肠球菌(vre)。风险因素微生物学与定植有显著相关性的是经验性使用抗生素、氨苄西林治疗、多次使用抗生素、既往住院治疗和糖尿病的存在。这些特征将有助于确定可能感染pOSS的患者,筛查耐药肠球菌,以便实施更严格的感染控制措施,防止这些微生物的传播。
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引用次数: 3
A study of the morbidity associated with episiotomy 会阴切开术并发症的研究
Pub Date : 2007-12-24 DOI: 10.4038/CJMS.V45I2.4853
D. Weerasekera, S. Udugama
Objective: To review the indications and the morbidity associated with episiotomy. Methods: Study was carried out in 450 consecutive women who had episiotomies at delivery during the period from 01-03-2002 to 01-09-2002 at University Unit, Colombo South Teaching Hospital. Women were interviewed within 24 hours of delivery and 6 weeks later. Data were collected using an interviewer administered questionnaire. Results: Out of a total of 876 mothers 51.4% had episiotomies at delivery and in 87% the indication was prevention of a possible perineal laceration. In 8% of women episiotomy was sutured later than 30 minutes from the time of performing. Although 10 ml of 1% lignocaine was infiltrated before performing and suturing the episiotomy, 80% of women complained of moderate to severe pain while performing the incision and 73% complained of moderate to severe pain during suturing. When seen six weeks later, 5% of patients complained of perineal pain and only 0.4 % had dyspareunia. Conclusion: Although episiotomy is generally considered to be a harmless procedure it causes moderate to severe pain in a majority of women. Adequate local analgesia, use of less-reactive suture material, emphasis on immediate suturing and post-partum analgesics may contribute to reduce the pain suffered by women following episiotomy.
目的:探讨会阴切开术的适应证及并发症。方法:对2002年3月1日至2002年9月1日在科伦坡南教学医院大学分院连续行外阴切开术的产妇450例进行研究。妇女在分娩24小时内和6周后接受了采访。数据收集采用采访者管理的问卷。结果:在876名母亲中,51.4%在分娩时进行了会阴切开术,87%的指征是预防可能的会阴撕裂。在8%的女性中,会阴切开术的缝合时间晚于手术时间30分钟。尽管在实施和缝合会阴切开术前浸润10ml 1%利多卡因,但80%的妇女在实施切口时抱怨中度至重度疼痛,73%的妇女在缝合时抱怨中度至重度疼痛。6周后,5%的患者抱怨会阴部疼痛,只有0.4%的患者有性交困难。结论:虽然外阴切开术通常被认为是一种无害的手术,但在大多数妇女中会引起中度至重度疼痛。适当的局部镇痛,使用反应性较低的缝合材料,强调立即缝合和产后镇痛可能有助于减少妇女在会阴切开术后遭受的疼痛。
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引用次数: 0
Anopheles tnaculatus (Theobald) and Anopheles elegans (James) breeding in water storage containers in Kandy, Sri Lanka 在斯里兰卡康提的储水容器中繁殖的特纳按蚊(Theobald)和秀丽按蚊(James)
Pub Date : 2007-12-24 DOI: 10.4038/CJMS.V45I2.4852
P. Kusumawathie, W. Fernando
A three month survey was carried out from August to October 2001 to identify anopheline mosquito breeding in artificial water storage containers in the Kandy District, Sri Lanka. Water storage tanks and barrels in 28 study sites covering 57 villages in 11 Divisional Director of Health Services (DDHS) areas were examined for mosquito immatures (larval instars and pupae). Anopheles ntaculatus (Theobald) and A. elegans (James) were shown to breed in these containers. Although these species are not known vectors of human malaria in Sri Lanka A. maculatus is a vector in neighboring countres. Also this species has been shown to support the sporogonic cycle of P. falciparum, in the laboratory; thus is considered as a potential vector of human malaria in Sri Lanka. With the impending water shortages, water storage in containers is bound to increase in the future. Thus condidering water storage containers as breeding places of potential vectors of human malaria is important in malaria control in Sri Lanka.
2001年8月至10月进行了为期3个月的调查,以查明斯里兰卡康提县人工储水容器中孳生的按蚊。对11个卫生服务司司长区57个村庄的28个研究地点的储水罐和储水桶进行了未成熟蚊子(幼虫和蛹)检查。结果显示,在这些容器中繁殖的有ntaculatus按蚊(Theobald)和elegans按蚊(James)。虽然这些物种在斯里兰卡不是已知的人类疟疾病媒,但斑斑拟蚊是邻国的病媒。在实验室中,这个物种也被证明支持恶性疟原虫的孢子生循环;因此被认为是斯里兰卡人类疟疾的潜在媒介。随着水资源短缺的迫近,未来容器中的储水量必然会增加。因此,将储水容器视为人类疟疾潜在媒介的孳生场所对斯里兰卡的疟疾控制具有重要意义。
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引用次数: 5
Direct nephrotoxic effect of Sri Lankan Russell's viper venom – an experimental study using in vitro models 斯里兰卡罗素毒蛇毒液的直接肾毒性作用-一项使用体外模型的实验研究
Pub Date : 2007-12-24 DOI: 10.4038/CJMS.V45I2.4851
M. Gunatilake, R. Jayakody, P. Angunawela, A. Tissera
Nephrotoxicity manifesting as acute renal failure is the principal cause of death in Russell's viper envenomation. The mechanisms responsible for nephrotoxicity are not clear and lack of experimental data in the Sri Lankan context prompted us to do this study. In the present experiments, the time related physiopathological changes in rabbits after injection of Russell's viper venom were studied using two in vitro models, viz. the isolated perfused kidney model and the kidney slice model. The isolated perfused kidney model experiments showed a significant time related reduction in renal function 4 hours after envenomation. This was associated with disturbances to the renal tubular cell integrity and renal pathology. Experiments using venom on kidney slices showed complete necrosis of the glomeruli and proximal convoluted tubular cells with the preservation of the basement membranes of tubular cells suggestive of direct damage caused by the venom. Data from these experiments are highly suggestive that Russell's viper venom causes renal damage mainly by a direct action on the kidney.
肾毒性表现为急性肾衰竭是罗素毒蛇中毒死亡的主要原因。导致肾毒性的机制尚不清楚,并且在斯里兰卡缺乏实验数据,这促使我们进行这项研究。本实验采用离体灌注肾模型和肾切片模型两种体外模型,研究了家兔注射毒蛇毒液后与时间相关的生理病理变化。离体灌注肾模型实验显示,中毒后4小时肾功能明显降低。这与肾小管细胞完整性和肾脏病理紊乱有关。在肾切片上使用毒液的实验显示肾小球和近曲小管细胞完全坏死,小管细胞的基底膜保存完好,这表明毒液造成了直接损伤。这些实验的数据高度暗示了罗素毒蛇毒液主要通过直接作用于肾脏而引起肾脏损伤。
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引用次数: 2
Prevalence of primary and secondary infertility in the Colombo District 科伦坡地区原发性和继发性不孕症的患病率
Pub Date : 2007-12-24 DOI: 10.4038/CJMS.V45I2.4854
S. Samarakoon, L. Rajapaksa, H. Seneviratne
Objective: To estimate the prevalence of primary and secondary infertility in the Colombo District Design: A cross sectional survey Setting: District of Colombo Sub jec t s : T w o thousand currently married women of the reproductive age group Measurements : Prevalence of primary and secondary infertility using the WHO definitions. When a woman has never conceived in spite of cohabitation and exposure to pregnancy for a period of twelve months, the condition was defined as p r imary infer t i l i ty . Seconda ry infertility was defined as being present when a woman had previously conceived but had been unable to conceive subsequently despite cohabi­ tation and exposure to pregnancy for a period of 24 months. If the woman had breast fed the previous infant, then exposure to pregnancy was calculated from the end of the period of lactation amenorrhoea. Results: The point prevalence of primary infer­ tility was estimated as 40.5 per 1000 married women of the reproductive age group, (95% C.L. 32.0 49.0 per 1000). The prevalence increased progressively with increasing current age and age at marriage of women and their partners and was higher among employed women. The point prevalence of secondary infertility was estimated as 160 per 1000 women of the repro­ ductive age group, (95% C.L. 143.9 176.0 per 1000). Increasing current age of women and their spouses, higher age at marriage of the male and low socio economic status were associated with increased prevalence. A history suggestive of post partum or post abortal infection was ob­ tained in 20% of persons who were secondarily infertile. Conclusions: Prevalence of primary infertility is low in the Colombo District, but amounts to an estimated 10,700 16,500 currently married women. The prevalence of secondary infertility is high, with post-partum and post-abortal infec­ tion contributing to a fifth of the cases.
目的:估计科伦坡地区原发性和继发性不孕症的患病率设计:横断面调查设置:科伦坡地区研究对象:2000名育龄已婚妇女测量:使用世界卫生组织定义的原发性和继发性不孕症的患病率。如果一名妇女同居并暴露在怀孕环境中12个月,但从未怀孕,则这种情况被定义为未怀孕。继发性不孕症被定义为女性先前怀孕,但在同居和接触怀孕24个月后仍无法怀孕。如果该妇女曾母乳喂养过前一个婴儿,则从哺乳期结束时闭经开始计算妊娠暴露。结果:初步推断生育能力的点患病率估计为40.5 / 1000育龄已婚妇女(95% C.L. 32.0 / 1000 49.0 / 1000)。发病率随着妇女及其伴侣当前年龄和结婚年龄的增加而逐渐增加,在就业妇女中发病率更高。继发性不孕症的点患病率估计为每1000名育龄妇女中有160人(95% C.L. 143.9 176.0 / 1000)。妇女及其配偶当前年龄的增加、男性结婚年龄的提高和社会经济地位的低下与发病率的增加有关。继发性不孕症患者中有20%有产后或流产后感染史。结论:科伦坡地区的原发性不孕症患病率较低,但估计有10,700 - 16,500名已婚妇女。继发性不孕症的患病率很高,产后和流产后感染占病例的五分之一。
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引用次数: 3
Smoking, addiction and promotion - an inquiry into perceptions of a group of adolescents in the Kandy Education Zone 吸烟、成瘾和促进——对康提教育区一群青少年看法的调查
Pub Date : 2005-06-24 DOI: 10.4038/CJMS.V48I1.4840
A. Alagiyawanna, P. Wickramaratne, D. Samarasekera
Introduction: Tobacco companies take vigorous action to promote smoking and to sustain the habit among young people. The companies mainly target students as they are in their formative stages of learning. Strong messages can influence their opinions leading to change in behavior. Objectives: To describe the attitudes regarding smoking among the students, their awareness regarding promotional strategies of tobacco companies and to identify the methods of acquiring knowledge regarding these strategies among Grade 12 students in the Kandy Educational Zone. Method: A descriptive cross sectional study was carried out. A multi stage cluster sampling method was adopted to select the study sample of 204. A pre-tested, self-administered questionnaire was used as the study instrument. • Results: More than 80% of both sexes perceived smoking as a negative social characteristic. Majority of the students (91.6%) thought that smokers have got addicted to it due to the lack of proper understanding. Awareness among, the students about the indirect promotional strategies of the tobacco companies was satisfactory as 47% of students knew about them. However, 41% had poor or very poor knowledge regarding these strategies. Mass media was shown to be a major source of acquiring knowledge on tobacco promotional strategies. Only one third of the students who participated in the study have been exposed to seminars/ workshops conducted by anti narcotic organizations. Conclusion: Students of our study population perceived smoking as a negative social characteristic. However, their knowledge regarding promotional strategies of tobacco companies needs improvement. Main mode of acquiring knowledge regarding promotional strategies was identified as mass media. The few who were exposed to seminars conducted by anti narcotic organizations were shown to have a significantly higher level of understanding on these strategies than the others.
引言:烟草公司采取有力行动,促进吸烟,并在年轻人中维持这一习惯。这些公司主要针对学生,因为他们正处于学习的形成阶段。强烈的信息可以影响他们的意见,从而改变他们的行为。目的:描述学生对吸烟的态度,他们对烟草公司促销策略的认识,并确定在康提教育区12年级学生中获取这些策略知识的方法。方法:采用描述性横断面研究。采用多阶段整群抽样方法,选取研究样本204份。使用预先测试的自我管理问卷作为研究工具。•结果:超过80%的男性和女性认为吸烟是一种消极的社会特征。大多数学生(91.6%)认为吸烟者对电子烟缺乏正确的认识。学生对烟草公司间接促销策略的知晓程度令人满意,有47%的学生知道烟草公司的间接促销策略。然而,41%的人对这些策略知之甚少或知之甚少。大众传播媒介已被证明是获取烟草促销战略知识的主要来源。参加这项研究的学生中,只有三分之一参加过禁毒组织举办的研讨会/讲习班。结论:我们研究人群的学生认为吸烟是一种消极的社会特征。然而,他们对烟草公司促销策略的了解还有待提高。大众传媒是获取促销策略知识的主要方式。少数参加过禁毒组织举办的讨论会的人对这些战略的了解程度明显高于其他人。
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引用次数: 0
期刊
Ceylon Journal of Medical Science
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