Biosensors and Bioelectronics: X最新文献_第6页

A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose 用于葡萄糖非酶定量的被动实验室- pcb微系统

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-07 DOI: 10.1016/j.biosx.2025.100670

Pavlos Menelaou , Yujiang Zhu , Anna Regoutz , Despina Moschou

This paper presents and successfully demonstrates a new form of passive Lab-on-printed circuit board (PCB) diagnostic platform for the quantification of glucose, as required for pumpless applications such as wearable diagnostic patches. The platform exploits copper oxide (CuO) nanoparticles for non-enzymatic, electrochemical glucose quantification, to achieve the high sensitivity and linear range of operation that is necessary for sweat or interstitial fluid sample analysis. As a result, the platform exhibits a low limit of detection (LoD) of 2.1 μM and a high sensitivity of 456 μA mM⁻¹·cm⁻², coupled with an excellent specificity against common glucose interfering species. The seamless integration of passive microfluidics and an electrochemical glucose biosensor is firstly outlined, and is fabricated using standard photolithography techniques in an up-scalable glucose quantification platform. The detection of glucose under two sample flow conditions is investigated in detail, including both static and dynamic conditions, revealing that when subject to a continuous flow the microsystem demonstrates an increase in sensitivity and a reduced linear range. This work demonstrates that our new passive Lab-on-printed circuit board (PCB) diagnostic platform can be successfully implemented under continuous sample flow conditions, and is therefore ideally suited to wearable diagnostic patch applications. In addition, the measured performance exceeds static flow approaches that have reported to date, including paper-based approaches.

本文介绍并成功演示了一种用于葡萄糖定量的新型无源印刷电路板实验室（PCB）诊断平台，用于无泵应用，如可穿戴诊断贴片。该平台利用氧化铜纳米颗粒进行非酶、电化学葡萄糖定量，实现了汗液或组织液样品分析所必需的高灵敏度和线性操作范围。结果表明，该平台具有2.1 μM的低检出限和456 μA mM−1·cm−2的高灵敏度，并且对常见的葡萄糖干扰物质具有良好的特异性。首先概述了无源微流体与电化学葡萄糖生物传感器的无缝集成，并在可扩展的葡萄糖定量平台上使用标准光刻技术制作。在两种样品流动条件下对葡萄糖的检测进行了详细的研究，包括静态和动态条件，揭示了当受到连续流动的影响时，微系统表现出灵敏度的增加和线性范围的减小。这项工作表明，我们新的无源印刷电路板实验室（PCB）诊断平台可以在连续样品流条件下成功实施，因此非常适合可穿戴诊断贴片应用。此外，测量的性能超过了迄今为止报道的静态流方法，包括基于纸张的方法。

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引用次数: 0

WGM Sensors for future diagnostics: small molecules to whole organisms 用于未来诊断的WGM传感器：从小分子到整个生物体

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-07 DOI: 10.1016/j.biosx.2025.100671

Wilhelm Eugen Andreas Krames , Defne Ilayda Dayi , Lars Kaiser , Oliver Riester , Michael Himmelhaus , René Csuk , Hans-Peter Deigner

In a world facing constant challenges from diseases and pathogens, rapid diagnosis is crucial. Whispering gallery mode (WGM) resonators present an advanced sensor technology suitable for the detection of various analytes. Like surface plasmon resonance (SPR), they offer highly sensitive and selective measurements, ideal for medical diagnostics. Traditional WGM resonators, however, are immobilized on surfaces near optical couplers, making their production complex and costly. Nevertheless, a more recent approach employs polystyrene microspheres localized to cavities in fluidic chips as resonators, simplifying preparation and functionalization and displaying enhanced performance. Here, we assess the applicability of those sensors to different analyte sizes, ranging from small molecules (<1000 Da) to whole organisms. By employing different detection strategies, we confirm the applicability of the WGM sensors for detecting a wide range of targets.

The study focuses on L. pneumophila, TEM-1 beta-lactamase, aztreonam, and meropenem as representatives of these three major groups. We show that the detection was successful for all chosen analytes, while small molecules required additional signal amplification through a sandwich approach. Results of the analysis of the protein measurements showed an LOD of 0.09 μM and an LOQ of 0.28 μM with a R² greater than 0.99. Additionally, a comparative measurement demonstrates that the WGM is more sensitive than the widely used gold standard SPR (LOD 0.34 μM; LOQ 1.12 μM). Taken together, our work demonstrates the applicability of the WGM sensors to pathogen detection, protein quantification or small molecule analysis with an improved sensitivity compared to current gold standard methods.

在一个不断面临疾病和病原体挑战的世界，快速诊断至关重要。窃窃廊模式（WGM）谐振器提供了一种先进的传感器技术，适用于各种分析物的检测。与表面等离子体共振（SPR）一样，它们提供了高度敏感和选择性的测量，是医学诊断的理想选择。然而，传统的WGM谐振器固定在光学耦合器附近的表面上，使其生产复杂且成本高昂。然而，最近的一种方法是将聚苯乙烯微球定位在流控芯片的空腔中作为谐振器，简化了制备和功能化，并显示出增强的性能。在这里，我们评估了这些传感器对不同分析物大小的适用性，从小分子（<1000 Da）到整个生物体。通过采用不同的检测策略，我们证实了WGM传感器对广泛目标检测的适用性。本研究以嗜肺乳杆菌、TEM-1 β -内酰胺酶、氨曲南和美罗培南为代表。我们表明，检测是成功的所有选定的分析物，而小分子需要额外的信号放大通过三明治的方法。分析结果显示，LOD为0.09 μM， LOQ为0.28 μM, R2 > 0.99。此外，对比测量表明，WGM比广泛使用的金标准SPR (LOD 0.34 μM；Loq为1.12 μm)。综上所述，我们的工作证明了WGM传感器在病原体检测、蛋白质定量或小分子分析方面的适用性，与目前的金标准方法相比，其灵敏度有所提高。

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引用次数: 0

Design and fabrication of smartphone based single paper lateral flow assay for early detection of PCOS biomarker 基于智能手机的PCOS生物标志物早期检测单纸侧流法的设计与制作

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-07 DOI: 10.1016/j.biosx.2025.100672

Aniket Nandi , Yash Kumar Gaur , Divyanshi Thakur , Ekta Daweshar , Kalicharan Sharma

Polycystic Ovary Syndrome (PCOS) is a multifactorial endocrine disorder with significant diagnostic challenges, especially in early stages. Current diagnostic methods are often complex, time-consuming, and dependent on laboratory-based infrastructure. This study presents the design and fabrication of a smartphone-assisted paper-based lateral flow assay (LFA) for the early detection of PCOS via the quantification of sex hormone-binding globulin (SHBG), a promising metabolic biomarker. Fluorescent nitrogen-doped carbon dots (CDs) were synthesized using a microwave-assisted method and conjugated with anti-SHBG antibodies via EDC/NHS chemistry. The LFA strips were constructed using Whatman filter paper, and hydrophobic barriers were drawn using permanent marker ink. A portable device equipped with a 365 nm UV LED and a smartphone camera enabled fluorescence detection. Recombinant SHBG antigen samples in phosphate-buffered saline (PBS) were tested across a concentration range of 80–4000 ng/mL. The system exhibited a strong linear response (R² = 0.9677) between SHBG concentration and fluorescence intensity, with a calculated limit of detection (LOD) and limit of quantification (LOQ) of 2.68 ng/mL and 19.93 ng/mL, respectively. The analytical range of 80–4000 ng/mL corresponds to 0.8–42.1 nmol/L, covers the clinical diagnostic threshold of SHBG (<36 nmol/L). These results demonstrate the feasibility of a low-cost, point-of-care diagnostic tool for early PCOS screening. Further validation with clinical samples is necessary for real-world deployment.

多囊卵巢综合征（PCOS）是一种多因素内分泌疾病，具有显著的诊断挑战，特别是在早期。目前的诊断方法往往复杂、耗时，并且依赖于实验室基础设施。本研究介绍了一种智能手机辅助的基于纸张的横向流动试验（LFA）的设计和制造，通过定量性激素结合球蛋白（SHBG），一种有前途的代谢生物标志物，用于PCOS的早期检测。采用微波辅助法制备了荧光氮掺杂碳点（CDs），并通过EDC/NHS化学与抗shbg抗体偶联。使用Whatman滤纸构建LFA条带，并使用永久标记墨水绘制疏水屏障。配备365纳米紫外LED和智能手机摄像头的便携式设备可以进行荧光检测。重组SHBG抗原样品在磷酸盐缓冲盐水（PBS）中测试，浓度范围为80-4000 ng/mL。该体系SHBG浓度与荧光强度呈良好的线性关系（R2 = 0.9677），计算出的检测限（LOD）和定量限（LOQ）分别为2.68 ng/mL和19.93 ng/mL。80 ~ 4000 ng/mL的分析范围对应0.8 ~ 42.1 nmol/L，达到SHBG的临床诊断阈值（36 nmol/L）。这些结果证明了一种低成本、即时诊断的多囊卵巢综合征早期筛查工具的可行性。在实际应用中，需要对临床样本进行进一步验证。

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引用次数: 0

Recent advances in tactile sensing technologies for human-robot interaction: Current trends and future perspectives 人机交互触觉传感技术的最新进展：当前趋势和未来展望

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-06 DOI: 10.1016/j.biosx.2025.100669

Hothefa Shaker Jassim , Yasmeena Akhter , Dhulfiqar Zoltán Aalwahab , Husam A. Neamah

Tactile sensing technology has witnessed remarkable advancements, significantly expanding its applications across robotics, medical diagnostics, and consumer electronics. This paper reviews the latest developments in tactile sensing technologies, with a particular focus on their critical role in enhancing human-robot interaction. It highlights advancements in mechanoreceptor technologies, emphasizing innovations in material science and sensor design that improve the functionality and adaptability of tactile sensors. The review critically examines the evolution of key sensing modalities—piezoresistive, capacitive, and piezoelectric sensors detailing their operational principles, performance improvements, and integration into robotics systems for intuitive and responsive interactions. Emerging trends in sensor flexibility, sensitivity, and energy efficiency are explored, addressing their importance for creating adaptive, sustainable solutions in human-centered robotics. Additionally, the paper discusses challenges such as scalability, durability, and cost-effectiveness, which remain barriers to widespread adoption in robotic and clinical applications. The work concludes with future research directions, advocating for the integration of tactile sensors with artificial intelligence to develop self-learning systems capable of sophisticated decision-making and seamless human-robot collaboration. This review aims to bridge the gap between current technologies and future possibilities, charting a path toward transformative innovations in tactile sensing for human-robot interaction.

触觉传感技术取得了显著进步，显著扩展了其在机器人、医疗诊断和消费电子领域的应用。本文综述了触觉传感技术的最新进展，重点介绍了触觉传感技术在增强人机交互中的重要作用。它突出了机械感受器技术的进步，强调了材料科学和传感器设计的创新，提高了触觉传感器的功能和适应性。这篇综述批判性地研究了关键传感模式的演变——压阻式、电容式和压电式传感器，详细介绍了它们的工作原理、性能改进以及与机器人系统的集成，以实现直观和响应式的交互。探讨了传感器灵活性，灵敏度和能源效率的新兴趋势，解决了它们在以人为中心的机器人技术中创建自适应，可持续解决方案的重要性。此外，本文还讨论了可扩展性、耐用性和成本效益等挑战，这些挑战仍然是机器人和临床应用广泛采用的障碍。工作总结了未来的研究方向，主张将触觉传感器与人工智能相结合，开发能够进行复杂决策和无缝人机协作的自学习系统。这篇综述旨在弥合当前技术和未来可能性之间的差距，为人机交互的触觉传感变革创新指明道路。

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引用次数: 0

A simple MOF-based nanosensor for bisphenol A detection: in vitro application in supernatant of human endothelial cell models 用于双酚A检测的简单mof纳米传感器：在人内皮细胞模型上清液中的体外应用

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-06 DOI: 10.1016/j.biosx.2025.100666

Lorena Garcia-Rodrigo , Cristina Tortolini , Araceli González-Cortés , Ilenia Ventura , Silvia Migliaccio , Elisabetta Ferretti , Riccarda Antiochia

This work describes a simple electrochemical nanosensor for bisphenol A (BPA) detection based on an iron metal organic framework (MIL-100(Fe)) immobilized onto a commercial multiwalled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) modified graphite screen printed electrode (GSPE). The electrochemical platform has been characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and SEM-EDX analysis. The resulting MIL-100(Fe)/AuNPs_MWCNTs/GSPE platform exhibited competitive analytical performances, including a wide dynamic range (0.1–100 μM), and a low detection limit (20 nM). Additionally, the proposed sensor demonstrated excellent selectivity, enabling accurate BPA detection in the presence of potential interferences. Lastly, the sensor has been successfully tested for in vitro BPA detection in the supernatant samples of human endothelial cellular models, Human Umbilical Vein Endothelial Cells (HUVECs) and Human Aortic Endothelial Cells (HAECs), exposed to BPA. These findings highlight the potential of the proposed nanosensor for the detection of BPA in supernatant cell fluids of individuals exposed to BPA at different concentrations and incubation times.

本工作描述了一种用于双酚a （BPA）检测的简单电化学纳米传感器，该传感器基于铁金属有机框架（MIL-100(Fe)）固定在商业多壁碳纳米管(MWCNTs)/金纳米颗粒（AuNPs）修饰的石墨丝网印刷电极（GSPE）上。利用循环伏安法（CV）、电化学阻抗谱（EIS）和SEM-EDX分析对电化学平台进行了表征。MIL-100(Fe)/AuNPs_MWCNTs/GSPE平台具有较好的分析性能，包括宽动态范围（0.1-100 μM）和低检出限（20 nM）。此外，所提出的传感器具有优异的选择性，能够在存在潜在干扰的情况下准确检测BPA。最后，该传感器已成功地在暴露于BPA的人内皮细胞模型、人脐静脉内皮细胞（HUVECs）和人主动脉内皮细胞（HAECs）的上清样品中进行了体外BPA检测。这些发现强调了所提出的纳米传感器在不同浓度和孵育时间暴露于双酚a的个体上清细胞液中检测双酚a的潜力。

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引用次数: 0

A wireless reader for detecting frequency shift of passive sensors 一种用于检测无源传感器频移的无线读取器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-05 DOI: 10.1016/j.biosx.2025.100668

Fariborz Mirlou , Emine Bardakci , Taher Abbasiasl , Levent Beker

A body area sensor network (bodyNET) consists of wearable and implantable sensors to monitor human physiological signals. While passive disposable, chip-free, and batteryless sensors have been widely studied, benchtop equipment is still required for data collection, limiting usage to laboratory settings. Efficient integration of passive wearable and implantable sensors with dedicated compact readout circuitry is crucial for enabling data acquisition outside controlled laboratory environments. Here, we present a miniaturized, flexible wireless reader (WiRe) designed for frequency-shifting passive sensors, which also incorporates power harvesting capability. WiRe is compatible with a wide variety of passive sensors, offering a versatile platform for diverse medical applications. We deployed WiRe across various passive sensor platforms to track frequency shifts associated with biological signals. Our results show that WiRe uniquely combines power harvesting with a flexible, portable readout interface, enabling effective and non-invasive detection of even minor frequency shifts in a frequency sweep range of 1–8 MHz with 100 kHz resolution for both in vitro and in vivo experiments.

人体区域传感器网络（bodyNET）由可穿戴和植入式传感器组成，用于监测人体生理信号。虽然无源一次性、无芯片和无电池传感器已经被广泛研究，但数据收集仍然需要台式设备，限制了实验室环境的使用。无源可穿戴和植入式传感器与专用的紧凑型读出电路的有效集成对于在受控实验室环境外实现数据采集至关重要。在这里，我们提出了一种小型化的，灵活的无线读取器（WiRe），设计用于移频无源传感器，它还包含了能量收集功能。WiRe与各种无源传感器兼容，为各种医疗应用提供了一个多功能平台。我们在各种无源传感器平台上部署了WiRe，以跟踪与生物信号相关的频率变化。我们的研究结果表明，WiRe独特地将能量收集与灵活的便携式读出接口相结合，能够在体外和体内实验中以100 kHz分辨率在1-8 MHz的频率扫描范围内有效且无创地检测即使是较小的频率偏移。

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引用次数: 0

Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor 利用3d打印电化学免疫传感器检测粪便血管活性肠多肽的年龄相关变化

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-08-04 DOI: 10.1016/j.biosx.2025.100667

Khalil K. Hussain , Chloe Miller , Mark Yeoman , Bhavik Anil Patel

Vasoactive intestinal polypeptide (VIP) is a 28-amino acid neuropeptide with a multitude of diverse physiological functions, including vasodilation, immune modulation, and most notable gastrointestinal regulation. At present, VIP is determined using biochemical assays, which are time-consuming and require complex multi-step sample preparation. Therefore, we focused on the development of the first electrochemical immunosensor for the detection of VIP in faecal pellets. Our VIP immunosensor was made by covalent immobilization of an anti-VIP antibody onto a thiophene-2-carboxylic acid conductive polymer layer, after deposition of gold nanostars onto a carbon black/polylactic acid 3D printed electrode. Scanning electron microscopy and electrochemical measurements confirmed layer-by-layer modification of the electrode to craft the VIP immunosensor. The immunosensor exhibited a linear range between 10 and 100 pM, with a limit of detection of 4.3 pM. The immunosensor was selective against electroactive compounds known to be present within the intestinal tract. We observed a reduction in VIP levels in faecal pellets from 24-month-old mice when compared to 12-month-old mice. This novel VIP electrochemical immunosensor can be an effective tool for diagnosis or prognosis of various diseases given the diverse physiological function of VIP.

血管活性肠多肽（VIP）是一种由28个氨基酸组成的神经肽，具有多种生理功能，包括血管舒张、免疫调节和最显著的胃肠调节。目前，VIP是通过生化分析来确定的，这种方法耗时且需要复杂的多步骤样品制备。因此，我们致力于开发首个用于检测粪便颗粒中VIP的电化学免疫传感器。我们的VIP免疫传感器是在炭黑/聚乳酸3D打印电极上沉积金纳米星后，将抗VIP抗体共价固定在噻吩-2-羧酸导电聚合物层上制成的。扫描电子显微镜和电化学测量证实了电极的逐层修饰，以制作VIP免疫传感器。该免疫传感器在10 ~ 100 pM之间呈线性范围，检测限为4.3 pM。该免疫传感器对已知存在于肠道内的电活性化合物具有选择性。我们观察到，与12个月大的小鼠相比，24个月大的小鼠粪便颗粒中的VIP水平有所降低。鉴于VIP的多种生理功能，这种新型VIP电化学免疫传感器可作为多种疾病诊断和预后的有效工具。

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引用次数: 0

Acrylate/GO composite film enabled optical microcavity relative humidity sensor with enhanced sensitivity by pump-detection technique 丙烯酸酯/氧化石墨烯复合膜光学微腔相对湿度传感器通过泵浦检测技术提高了灵敏度

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-07-31 DOI: 10.1016/j.biosx.2025.100665

Senpeng Zhang, Bo Dong, Zhuojun Wang, Ziheng Yu, Yulong Wang

An acrylate/graphene oxide (GO) composite film enabled optical microcavity relative humidity (RH) sensor with enhanced sensitivity by pump-detection technique is presented. An acrylate/GO composite film serves as both the sensitive film and reflection film of an open optical microcavity, which is fabricated by 3D printing on the end face of a single-mode fiber. A pump-detection technique is used to improve the sensitivity of the sensor. Experimental results show that its sensitivity is only 151.11 p.m./%RH without the 980-nm pump light. In contrast, its sensitivity is significantly improved after introducing the 980-nm pump light, and its sensitivity reaches 2.4 times as much as that of the unpumped sensor. Moreover, it exhibits fast response characteristics with humidity response time and recovery time of 0.32 s and 1.40 s, respectively. In real-world application tests, the sensor effectively distinguishes normal, rapid, slow, and apnea breathing patterns, demonstrating strong potential for medical and sports-related respiratory monitoring.

提出了一种利用泵浦检测技术提高灵敏度的丙烯酸酯/氧化石墨烯复合膜光学微腔相对湿度传感器。丙烯酸酯/氧化石墨烯复合薄膜作为开放光学微腔的敏感膜和反射膜，通过3D打印在单模光纤端面上制备。采用泵浦检测技术提高了传感器的灵敏度。实验结果表明，在没有980 nm泵浦光的情况下，其灵敏度仅为151.11 pm /%RH。相比之下，引入980 nm泵浦光后，其灵敏度明显提高，灵敏度达到未泵浦传感器的2.4倍。湿度响应时间和恢复时间分别为0.32 s和1.40 s，具有快速响应特性。在实际应用测试中，该传感器可有效区分正常、快速、缓慢和呼吸暂停呼吸模式，显示出在医疗和运动相关呼吸监测方面的强大潜力。

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引用次数: 0

Fluorescence sensing platform based on graphene oxide nucleic acid interaction for detecting mercury ions, bisphenol A, and target DNA 基于氧化石墨烯核酸相互作用的荧光传感平台，用于检测汞离子、双酚A和靶DNA

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-07-28 DOI: 10.1016/j.biosx.2025.100662

Xiangyu Xu, Yinan Guo, Xiong Zhou, Shuai Xu, Heng Xu

A multifunctional fluorescence sensing platform based on graphene oxide (GO) was developed to meet the rapid detection needs of environmental pollutants. Three specific mechanisms were employed to achieve highly sensitive detection. One was to use the T-Hg2+ - T structure to induce the dissociation of double stranded DNA from GO surface, achieving Hg2+ detection with a linear range of 100–1500 nM and a detection limit of 0.110 μ M. The second method was to release the trigger chain through the dissociation of bisphenol A (BPA) adapter complementary chain complex, combined with GO fluorescence quenching characteristics to detect BPA, with a detection limit of 0.5035 μ g/mL. The third method used Exo III enzyme to cleave double stranded DNA for signal amplification, combined with GO differential adsorption detection of nucleic acid fragments to detect target DNA, corresponding to a linear range of 0.01–0.4 nM and a detection limit of 11.2 pM. The spiked recovery rates of the three methods in wastewater/serum samples were 96.82 %–107.49 %, with a relative standard deviation of<3.47 %. The above research method combined nucleic acid recognition characteristics with GO signal regulation function through modular design, providing a new strategy for on-site detection of environmental pollutants.

为满足环境污染物的快速检测需求，研制了一种基于氧化石墨烯（GO）的多功能荧光传感平台。采用三种特定的机制来实现高灵敏度的检测。一种是利用T-Hg2+ - T结构诱导氧化石墨烯表面双链DNA解离，实现Hg2+检测，线性范围为100-1500 nM，检出限为0.110 μ m。二种方法是通过双酚a （BPA）接合互补链络合物解离释放触发链，结合氧化石墨烯荧光猝灭特性检测BPA，检出限为0.5035 μ g/mL。第三种方法利用Exo III酶裂解双链DNA进行信号扩增，结合GO差分吸附核酸片段检测靶DNA，对应线性范围为0.01 ~ 0.4 nM，检出限为11.2 pM。3种方法在废水/血清样品中的加标回收率为96.82% ~ 107.49%，相对标准偏差为3.47%。上述研究方法通过模块化设计将核酸识别特性与GO信号调节功能相结合，为环境污染物的现场检测提供了一种新的策略。

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引用次数: 0

Hydrogel-based multiplexed high-risk human papillomavirus DNA/RNA detection for cervical cancer screening 基于水凝胶的多重高危人乳头瘤病毒DNA/RNA检测用于宫颈癌筛查

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-07-28 DOI: 10.1016/j.biosx.2025.100664

Morteza Azizi , Jae-Sang Hong , Joshua Spitzberg , Ralph Weissleder , Cesar M. Castro , Hyungsoon Im

Rapid triaging of high-risk human papillomavirus (HPV) infections, especially in resource-limited regions, can reduce cervical cancer deaths by identifying high-risk patients and minimizing pathology bottlenecks and overtreatment. Here, we developed a simple, rapid method for parallel detection of HPV DNA and RNA subtypes associated with a high risk of developing cervical cancer. Specifically, we applied loop-mediated isothermal nucleic acid amplification (LAMP) in a hydrogel-based platform (LAMPGel) for HPV DNA or RNA detection using distinct hydrogel chambers within a single chip. The LAMPGel platform locally restricts single HPV gDNA or mRNA strands with a LAMP cocktail, which is then distributed within a thin hydrogel to locally form single fluorescent spots after a short isothermal incubation of <30 min. Detecting and counting these spots identifies HPV infection, viral load, and, more importantly, RNA expression as a predictive marker for cervical cancer development. LAMPGel is a sensitive, specific, easy-to-use, and rapid method for a point-of-care (POC) platform for potential applications in low- and middle-income countries once the technology is further validated with clinical samples in those settings. Furthermore, it could find broader applications in POC nucleic acid assays.

对高危人乳头瘤病毒（HPV）感染进行快速分诊，特别是在资源有限的地区，可以通过识别高危患者和尽量减少病理瓶颈和过度治疗来减少宫颈癌死亡。在这里，我们开发了一种简单、快速的方法，用于平行检测与宫颈癌高风险相关的HPV DNA和RNA亚型。具体来说，我们在基于水凝胶的平台（LAMP）中应用环介导的等温核酸扩增（LAMP），在单个芯片中使用不同的水凝胶腔进行HPV DNA或RNA检测。LAMPGel平台用LAMP鸡尾酒局部限制单个HPV gDNA或mRNA链，然后将其分布在薄水凝胶中，在30分钟的短暂等温孵卵后局部形成单个荧光点。检测和计数这些斑点可识别HPV感染，病毒载量，更重要的是，RNA表达作为宫颈癌发展的预测标志物。LAMPGel是一种灵敏、特异、易于使用和快速的方法，可用于医疗点（POC）平台，一旦该技术在这些国家的临床样本中得到进一步验证，就有可能在这些国家得到应用。此外，它在POC核酸分析中有更广泛的应用前景。

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引用次数: 0