Biosensors and Bioelectronics: X最新文献_第10页

Innovative Ag@Au nanozyme-enhanced organic photoelectrochemical transistor for ultrasensitive ochratoxin A detection 创新Ag@Au纳米酶增强有机光电化学晶体管用于超灵敏赭曲霉毒素A检测

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-21 DOI: 10.1016/j.biosx.2025.100612

Shusheng Wei, Yuchen Shen, zhanpeng Zhang, Juan Wang

Organic bioelectronic devices are developing as adaptable platforms for advanced biosensing applications, such as wearable sensors, neural interfaces and tissue engineering, due to their remarkable flexibility, mobility, ease of manufacture, and biocompatibility. The article presents a unique organic photoelectrochemical transistor (OPECT) sensor, combined with an Ag@Au nanozyme-mediated catalytic precipitation mechanism, creating an ultrasensitive detection platform for Ochratoxin A (OTA). The ZnO/ZnFe₂O₄ heterostructure is established as a novel gating module. The ZnFe₂O₄ layer may boost electrolyte interaction and light accessibility to the ZnO nanoarray, thereby modulating the response of the polymeric poly (3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel, which can be monitored through the channel current. In conjunction with aptamer sensing, the Ag@Au nanozyme, exhibiting peroxidase-mimicking activity, catalyzes the oxidation of 4-chloro-1-naphthol (4-CN), leading to the formation of an insoluble precipitate on the gate electrode surface, which diminishes the photocurrent and modifies the transistor response. The OPECT sensor demonstrates outstanding analytical capabilities for OTA, featuring a wide dynamic range from 10⁻⁵ ng/mL to 10 ng/mL and a detection limit of 0.0206 pg/mL. The advancement of this OPECT sensor offers potential for employing organic photoelectrochemical transistors as a high-performance platform for OTA detection.

有机生物电子器件由于其卓越的灵活性、移动性、易于制造和生物相容性，正在发展成为先进生物传感应用的适应性平台，如可穿戴传感器、神经接口和组织工程。本文提出了一种独特的有机光电电化学晶体管（OPECT）传感器，结合Ag@Au纳米酶介导的催化沉淀机制，建立了赭曲霉毒素a （OTA）的超灵敏检测平台。建立了ZnO/ZnFe2O4异质结构作为一种新型的门控模块。ZnFe2O4层可以增强电解质相互作用和ZnO纳米阵列的光可及性，从而调节聚合物聚（3,4-乙烯二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT:PSS）通道的响应，这可以通过通道电流来监测。与适体感应相结合，Ag@Au纳米酶表现出模仿过氧化物酶的活性，催化4-氯-1-萘酚（4-CN）的氧化，导致在栅极表面形成不溶性沉淀物，从而减少光电流并改变晶体管响应。OPECT传感器具有出色的OTA分析能力，具有10 - 5 ng/mL至10 ng/mL的宽动态范围，检测限为0.0206 pg/mL。这种OPECT传感器的进步为采用有机光电电化学晶体管作为OTA检测的高性能平台提供了潜力。

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引用次数: 0

High-throughput and label-free screening of red blood cell stiffness: A study of sickle cell disease 红细胞硬度的高通量和无标记筛选：镰状细胞病的研究

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-20 DOI: 10.1016/j.biosx.2025.100616

Saurabh Kaushik , Arkabrata Mishra , Roshan Ross , Sweta Srivastava , Cecil R. Ross , Gautam V. Soni

Understanding the morphological and mechanical changes in cells are important for diagnostic and treatment methods in various diseases. In sickle cell disease (SCD), the mutated hemoglobin (HbS) aggregates inside the red blood cells (RBCs), making them rigid and, in extreme cases, sickle-shaped, resulting in anemia, episodes of pain, and multiple organ damage. Existing techniques are too costly and insensitive since the effect of the HbS gene (heterozygous and homozygous) is variable both in prevalence and clinical manifestations. In this work, we present a label-free, cost-effective, high-throughput electro-fluidic technique to study changes in the mechanical and morphological characteristics of RBCs. We validate our device by quantitatively comparing the mechanical properties of RBCs as a function of stiffness-altering drug (Latrunculin-A) with measurements using AFM. We demonstrate the on-site application of our system by screening SCD patients based on their RBC stiffness changes. The signatures of patient-specific heterogeneity in the RBC mechanical properties may help in monitoring clinical variability and identification of high-risk patients along with targeted therapies. The versatility of our measurements opens the whole cell stiffness as a preliminary screening biomarker in other haematological conditions, tumor cell identification, in veterinary sciences as well as in evaluating hydrogel technologies.

了解细胞的形态和力学变化对各种疾病的诊断和治疗方法具有重要意义。在镰状细胞病（SCD）中，突变的血红蛋白（HbS）聚集在红细胞（rbc）内，使它们变得坚硬，在极端情况下，呈镰状，导致贫血、疼痛发作和多器官损伤。由于HbS基因（杂合子和纯合子）的影响在患病率和临床表现上都是可变的，现有的技术过于昂贵且不敏感。在这项工作中，我们提出了一种无标签、低成本、高通量的电流体技术来研究红细胞的力学和形态特征的变化。我们通过定量比较红细胞的力学特性作为刚度改变药物（Latrunculin-A）的函数与使用AFM测量来验证我们的设备。我们通过筛选SCD患者的RBC硬度变化来展示我们的系统的现场应用。红细胞力学特性的患者特异性异质性特征可能有助于监测临床变异性和识别高风险患者以及靶向治疗。我们测量的多功能性打开了整个细胞刚度作为其他血液学条件，肿瘤细胞鉴定，兽医科学以及评估水凝胶技术的初步筛选生物标志物。

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引用次数: 0

2D nanomaterials in biosensing: Synthesis, characterization, integration in biosensors and their applications 生物传感中的二维纳米材料：生物传感器的合成、表征、集成及其应用

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-20 DOI: 10.1016/j.biosx.2025.100615

Desmond Lutomia , Renu Poria , Deepak Kala , Preeti Garg , Rupak Nagraik , Ankur Kaushal , Shagun Gupta , Deepak Kumar

Recent advances in the synthesis of functional nanomaterials and precisely engineered nanostructures have opened up new avenues for the fabrication of viable biosensors for field analysis. Two-dimensional (2D) nanomaterials provide unique hierarchical structures, high surface area, and layered configurations with multiple length scales and porosity, and the possibility to create functionalities for targeted recognition at their surface. In addition to providing extra features like structural color, ordered morphological features, and the capacity to detect and react to external stimuli, such hierarchical structures provide opportunities to tune the characteristics of materials. Combining these distinctive qualities of the various nanostructure types and using them as a foundation for bimolecular assemblies can yield biosensing platforms with enhanced robustness, sensitivity, and selectivity for the detection of a wide range of analytes, as well as targeted recognition and transduction properties that can have a positive impact on numerous fields. This review describes the classification, synthesis and characterization of 2D nanomaterials and their functionalization. In addition, the merits of the 2D nanomaterials and their applications in health, environmental monitoring and food safety and control are covered. The final part anticipates the advancement of 2D nanomaterials in biosensors, challenges and future directions of 2D nanomaterials in biosensors.

功能纳米材料的合成和精确工程纳米结构的最新进展为制造可行的现场分析生物传感器开辟了新的途径。二维（2D）纳米材料提供了独特的分层结构、高表面积和具有多种长度尺度和孔隙度的分层配置，并有可能在其表面创建目标识别功能。除了提供额外的功能，如结构颜色，有序的形态特征，以及检测和响应外部刺激的能力外，这种分层结构还提供了调整材料特性的机会。结合不同纳米结构类型的这些独特品质，并将其作为双分子组装的基础，可以产生具有增强鲁棒性，灵敏度和选择性的生物传感平台，用于检测广泛的分析物，以及可在许多领域产生积极影响的靶向识别和转导特性。本文综述了二维纳米材料的分类、合成、表征及其功能化。此外，还介绍了二维纳米材料的优点及其在健康、环境监测和食品安全与控制方面的应用。最后对二维纳米材料在生物传感器领域的研究进展、面临的挑战和未来发展方向进行了展望。

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引用次数: 0

An innovative and mass-sensitive quartz tuning fork (QTF) biosensor for GFAP detection: A novel approach for traumatic brain injury diagnosis 用于GFAP检测的创新性质量敏感石英音叉（QTF）生物传感器：一种创伤性脑损伤诊断的新方法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-18 DOI: 10.1016/j.biosx.2025.100614

Burcu Özcan , İnci Uludağ Anıl , Mehmet Altay Ünal , Fikret Arı , Mustafa Kemal Sezgintürk , Sibel Ayşıl Özkan

The early diagnosis and management of traumatic brain injury (TBI) are dependent upon the early and precise detection of glial fibrillary acidic protein (GFAP). In this investigation, a novel biosensor based on quartz tuning forks (QTF) was introduced and functionalized with 11-mercaptoundecanoic acid (11-MUA). This biosensor is designed to facilitate the highly sensitive and selective detection of GFAP in human serum. In contrast to conventional neuroimaging methods, which are resource-intensive and frequently inaccessible in emergency situations, this innovative biosensor offers a portable, cost-effective, and efficient alternative for rapid GFAP measurement. The detection range of the system is 0.05 fg mL⁻¹ to 25 fg mL⁻¹. The Atomic Force Microscopy (AFM) was utilized to visualize the morphology of the QTF surface during the immobilization steps of the sensor. The developed biosensor presented advantages such as ability to determine GFAP concentrations at femtogram level, reproducibility and repeatability (standard deviation: ±0.0935966 Hz, and coefficient of variation: 7.91 %). This study highlights a significant progression in biosensing technology, providing an exceptionally sensitive and scalable platform for diagnosing neurological disorders, with potential uses in point-of-care environments.

创伤性脑损伤（TBI）的早期诊断和治疗依赖于胶质原纤维酸性蛋白（GFAP）的早期准确检测。本文介绍了一种基于石英音叉（QTF）的新型生物传感器，并采用11-巯基十四酸（11-MUA）进行了功能化。该生物传感器设计用于促进高灵敏度和选择性地检测人血清中的GFAP。传统的神经成像方法是资源密集型的，在紧急情况下经常无法使用，与之相反，这种创新的生物传感器为快速测量GFAP提供了一种便携式、经济高效的替代方案。系统检测范围为0.05 fg mL−1 ~ 25 fg mL−1。利用原子力显微镜（AFM）观察传感器固定过程中QTF表面的形貌。所研制的生物传感器具有飞图水平测定GFAP浓度、重现性和可重复性（标准偏差：±0.0935966 Hz，变异系数：7.91%）等优点。这项研究强调了生物传感技术的重大进展，为诊断神经系统疾病提供了一个异常敏感和可扩展的平台，在护理点环境中具有潜在的用途。

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引用次数: 0

Real-time monitoring of ssDNA binding using a fiber optic LSPR microfluidic platform 利用光纤LSPR微流控平台实时监测ssDNA结合

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-18 DOI: 10.1016/j.biosx.2025.100613

Vivek Semwal , Asbjørn Meldgaard Moltke , Ole Bang , Jakob Janting

In this paper, we present the development of a localized surface plasmon resonance (LSPR) sensor for the detection of single-stranded DNA (ssDNA). The LSPR chip was fabricated using gold nanoparticles (AuNPs) with a diameter of 80 nm. It was integrated with a microfluidic chamber to ensure stable measurements. We employed data processing techniques to fit the absorbance curve and extract the resonance wavelength, significantly reducing noise and achieving a 100-fold improvement in signal quality. The fabricated LSPR chips demonstrated a bulk refractive index sensitivity of approximately 85–90 nm/RIU. This paper outlines a robust methodology for reliable LSPR measurements based on cheap and readily accessible instruments. We have shown successfully real-time binding between Poly(T₂₀) and Poly(A₂₀), sensitive down to a concentration of 2 nM, while maintaining signal fluctuations 10 times lower than the shift in resonance wavelength without using any complex signal amplification technique. The sensor exhibits a limit of detection (LOD) of 0.75 nM. The proposed method shows potential for high-sensitivity and reliable real-time detection of smaller biomolecules, environmental pollutants, foodborne pathogens, toxins, and disease biomarkers.

本文介绍了一种用于单链DNA检测的局部表面等离子体共振（LSPR）传感器的研制。LSPR芯片采用直径为80 nm的金纳米颗粒（AuNPs）制备。它集成了一个微流控室，以确保稳定的测量。我们采用数据处理技术拟合吸光度曲线并提取共振波长，显著降低了噪声，使信号质量提高了100倍。所制备的LSPR芯片的整体折射率灵敏度约为85 ~ 90 nm/RIU。本文概述了一种可靠的基于廉价和容易获得的仪器的LSPR测量方法。我们已经成功地展示了Poly（T20）和Poly（A20）之间的实时结合，灵敏度低至2 nM的浓度，同时保持信号波动比共振波长的位移低10倍，而无需使用任何复杂的信号放大技术。该传感器的检测限（LOD）为0.75 nM。该方法显示出高灵敏度和可靠的实时检测小生物分子、环境污染物、食源性病原体、毒素和疾病生物标志物的潜力。

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引用次数: 0

Hot or not: Quantifying isothiocyanates in plants, soil, and other media 热或不热：定量植物、土壤和其他介质中的异硫氰酸酯

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-15 DOI: 10.1016/j.biosx.2025.100599

Octavia Hogaboam , Viola A. Manning , Catherine L. Reardon , Kristin M. Trippe

Isothiocyanates (ITCs) are bioactive compounds produced by plants in the Brassicales that serve as natural defense mechanisms against pests and pathogens, and provide sharp, hot, and pungent flavors to plants like wasabi, mustards, and horseradish. In agricultural settings, natural and synthetic ITCs are used to biofumigate soils prior to planting; however, because residual ITCs can inhibit germination or plant growth, (i.e. soils are “hot”), caution is necessary when fields are replanted. Current methodologies that measure ITCs are labor intensive and require expensive instrumentation. Therefore, there is a critical need for rapid, reliable, and inexpensive methods that detect ITCs in a variety of plant and soil matrices. This study describes the development and validation of the SaxAPIL biosensor, which uses an ITC-responsive promoter to drive expression of a bioluminescent reporter in Pseudomonas fluorescens SBW25 to quantify ITCs. Our results indicate that SaxAPIL can be used in a high throughput microplate-based assay to detect ITCs in a dose-dependent manner in solutions, plant- and seed meal-derived extracts, and soils amended with green manure or seed meals. Our results clearly demonstrate that SaxAPIL is a robust biosensor for the detection and quantification of aliphatic ITCs in plants and soil. The methodology presented here may be adapted to provide more efficient and less expensive methods to measure ITCs in industrial, health, and life science applications.

异硫氰酸酯（ITCs）是由十字花科植物产生的生物活性化合物，作为对抗害虫和病原体的天然防御机制，并为山葵、芥末和辣根等植物提供辛辣、辛辣和辛辣的味道。在农业环境中，天然和合成的ITCs用于种植前对土壤进行生物熏蒸；然而，由于残留的ITCs会抑制发芽或植物生长（即土壤“热”），因此在重新种植田地时必须谨慎。目前测量ITCs的方法是劳动密集型的，并且需要昂贵的仪器。因此，迫切需要快速、可靠和廉价的方法来检测各种植物和土壤基质中的ITCs。本研究描述了SaxAPIL生物传感器的开发和验证，该传感器使用一个icc响应启动子来驱动荧光假单胞菌SBW25中生物发光报告基因的表达，以量化icc。我们的研究结果表明，SaxAPIL可用于基于微孔板的高通量检测，以剂量依赖的方式检测溶液、植物和种子粕提取物，以及用绿肥或种子粕改性的土壤中的ITCs。我们的研究结果清楚地表明，SaxAPIL是一种强大的生物传感器，用于检测和定量植物和土壤中的脂肪族ITCs。本文提出的方法可以进行调整，以提供更有效和更便宜的方法来衡量工业、卫生和生命科学应用中的国际技术含量。

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引用次数: 0

Combining dopamine and glucose sensings on paper devices for the metabolic study of neurosecretion 结合多巴胺和葡萄糖在纸装置上的传感神经分泌代谢研究

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-15 DOI: 10.1016/j.biosx.2025.100601

Rémi F. Dutheil , Dabeaurard Tho , Iman Pitroipa , Raphaël Trouillon

Glucose, the main source of energy of the human body, and dopamine, a major neurotransmitter, are two analytes widely investigated in the study of the brain. In many pathologies, a dysfunction in their metabolic pathways can be observed, leading to neurological disorders. Better understanding the interplays between secretion and cellular metabolism is critical to better address these diseases. In this study, we study the simultaneous detection of glucose consumption and dopamine secretion using a paper-based electrode (PBE). An electrode made of carbon nanotube-coated paper was functionalized with platinum nanoparticles and glucose oxidase to gain sensitivity towards glucose. Maximal current density (J

_{m a x}

) and Michaelis–Menten constant (K

_{m}

) were respectively

12.4 \pm 2.0 μ

A.mm⁻² and 7.6 ± 1.5 mM for the glucose calibration. The results suggest that dopamine secretion and glucose consumption can be measured in a neuron cell model using the developed paper-based sensor. After stimulating the cells, glucose and dopamine concentration decreased by 1.1 mM and increased by

7.1 μ

M, respectively. In addition, to confirm the sensor’s detection of dopamine secretion, the impact of L-DOPA, a dopamine precursor, was tested. Dopamine secretion increased two-fold after incubation with L-DOPA, while glucose consumption remained unchanged. This opens new opportunities for quantitative, rapid multianalyte sensing of the chemical inputs and outputs of cellular mechanisms with an easy-to-use and affordable device.

葡萄糖是人体能量的主要来源，多巴胺是一种主要的神经递质，是大脑研究中广泛研究的两种分析物。在许多病理中，可以观察到代谢途径的功能障碍，导致神经系统疾病。更好地了解分泌和细胞代谢之间的相互作用对于更好地治疗这些疾病至关重要。在这项研究中，我们研究了使用纸基电极（PBE）同时检测葡萄糖消耗和多巴胺分泌。利用纳米铂纳米粒子和葡萄糖氧化酶对碳纳米管涂布纸电极进行功能化，提高了电极对葡萄糖的敏感性。最大电流密度（Jmax）和Michaelis-Menten常数（Km）分别为12.4±2.0μA。mm−2和7.6±1.5 mm用于葡萄糖校准。结果表明，多巴胺分泌和葡萄糖消耗可以在神经元细胞模型中使用开发的基于纸张的传感器进行测量。刺激细胞后，葡萄糖和多巴胺浓度分别降低1.1 mM和升高7.1μM。此外，为了证实传感器对多巴胺分泌的检测，还测试了多巴胺前体L-DOPA的影响。与左旋多巴孵育后，多巴胺分泌增加了两倍，而葡萄糖消耗保持不变。这为使用易于使用和负担得起的设备对细胞机制的化学输入和输出进行定量，快速多分析物传感开辟了新的机会。

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引用次数: 0

Mechanosynthesis and thermal bio–sensing of beryllium–based molecularly imprinted polymers 铍基分子印迹聚合物的机械合成和热生物传感

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-14 DOI: 10.1016/j.biosx.2025.100605

Ana I. Furtado , Joseph W. Lowdon , Kasper Eersels , Bart van Grinsven , Adriana Cruz , Jacinta Serpa , Vasco D.B. Bonifácio , Raquel Viveiros , Teresa Casimiro

The adsorption of amino acids on electrode surfaces is pertinent to understanding the interfacial behaviours of biological molecules and addressing industrial challenges associated with their purification and monitoring in downstream processes. Molecularly imprinted polymers (MIPs) are ideal candidates for targeted molecular recognition. Metals offer significant potential for enhancing biological molecule recognition by enabling the creation of selective binding sites within polymeric matrices through molecular imprinting. The metal mediated coordination between the monomer and the biomolecule used as template greatly enhances both the affinity and selectivity of molecular recognition. Herein, beryllium–based natural monomers (curcumin and lawsone) were synthesized and applied as functional monomers in the synthesis of MIPs using the amino acid L–leucine (LEU) as template. Mechanochemistry (ball milling) was chosen as key methodology for the synthesis of both the beryllium–based monomers and MIP (BeMIPMs) fabrication. Subsequently, supercritical CO₂ (scCO₂) technology was used for efficiently desorb of the template, yielding vacant receptors. These two green technologies allowed the preparation of BeMIPMs as ready–to–use and stable dry polymeric powders. The prepared BeMIPM particles were then incorporated into a thermally conductive layer via micro–contact deposition. Their response towards LEU and analogues molecules was analysed using the heat–transfer method (HTM), and their performance was compared to the non–imprinted polymer (BeNIPMs) reference. The generated biosensor was found to have an optimal linear range of 0.30–0.93 mM and LoD of 0.16 mM (obtained by the 3σ method), while also being selective when comparing the thermal response to other analogues molecules (IF_effect-LEU = 1.6–1.8 vs. IF_{analogues-molecule} = 0.5–1.5). BeMIPM shows a promising performance for the monitoring of LEU in purification processes due to its thermal response, inclusive in real samples, offering a low–cost thermal platform for monitoring specific amino acids in complex industrial matrices.

氨基酸在电极表面的吸附有助于理解生物分子的界面行为，并解决与下游过程中的纯化和监测相关的工业挑战。分子印迹聚合物（MIPs）是靶向分子识别的理想候选者。金属通过分子印迹在聚合物基质中产生选择性结合位点，为增强生物分子识别提供了巨大的潜力。金属介导的分子与作为模板的生物分子之间的配位极大地提高了分子识别的亲和力和选择性。本文合成了以铍为基础的天然单体（姜黄素和劳索酮），并以l -亮氨酸（LEU）为模板，将其作为功能单体应用于mip的合成。机械化学（球磨）被选为合成铍基单体和制备MIP （bemipm）的关键方法。随后，利用超临界CO2 （scCO2）技术对模板进行高效解吸，得到空受体。这两种绿色技术使得bemipm作为即用型和稳定的干燥聚合物粉末的制备成为可能。然后通过微接触沉积将制备的BeMIPM颗粒整合到导热层中。利用热传递方法（HTM）分析了它们对低浓铀和类似物分子的反应，并将它们的性能与非印迹聚合物（BeNIPMs）进行了比较。所制备的生物传感器的最佳线性范围为0.30-0.93 mM， LoD为0.16 mM（通过3σ方法获得），同时在与其他类似物分子进行热响应比较时也具有选择性（ifeeffect - leu = 1.6-1.8 vs. IFanalogues-molecule = 0.5-1.5）。BeMIPM在纯化过程中表现出良好的性能，因为它的热响应，包括在实际样品中，为监测复杂工业基质中的特定氨基酸提供了低成本的热平台。

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引用次数: 0

Sensitive and selective electrochemical biosensor based on Manganese(II) complex for simultaneous determination of adenine and guanine from clinical samples and DNA extract samples 基于锰（II）配合物的电化学生物传感器用于同时测定临床样品和DNA提取样品中的腺嘌呤和鸟嘌呤

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-14 DOI: 10.1016/j.biosx.2025.100603

Melaku Metto , Alemu Tesfaye , Minaleshewa Atlabachew , Abayneh Munshea , Atakilt Abebe

The purines guanine and adenine are essential building blocks for nucleic acids and influence numerous biochemical processes in organisms. Elevated levels of these compounds in the bloodstream can indicate conditions such as cancer and provide insights into cellular energy status, tissue degradation, and enzyme malfunctions in metabolic pathways.

This study focuses on developing a voltammetric sensor through the electropolymerization of a tetraresorcinatemanganate (II) complex on a glassy carbon electrode (poly(Mn(HR)₄)/GCE), which was thoroughly characterized. The poly(Mn(HR)₄)/GCE exhibited distinct, well-defined irreversible oxidative peaks for adenine and guanine. The peak currents displayed strong linearity with analyte concentrations within the 0.01–300 μM range, boasting a detection limit of 66.54 and 9.1 nm and a limit of quantifications of 221.80 and 30.23 nm, respectively. The sensor was successfully employed to detect adenine and guanine in urine, clinical blood serum, and DNA extract samples, with spike recovery rates in these samples reaching the range of 96–104 %. The interference recovery results showcased an error rate of less than 6 %, highlighting the method's superior lower detection limit and broader dynamic range compared to existing techniques. These findings underscore the potential practicality of the proposed approach for accurately determining adenine and guanine in diverse real samples with intricate matrices.

嘌呤鸟嘌呤和腺嘌呤是核酸的基本组成部分，影响生物体的许多生化过程。血液中这些化合物水平的升高可以提示癌症等疾病，并提供对细胞能量状态、组织降解和代谢途径中的酶故障的见解。本研究的重点是通过在玻碳电极（poly(Mn(HR)4)/GCE）上电聚合四硅酸锰酸盐（II）配合物来开发伏安传感器，并对其进行了全面的表征。聚(Mn(HR)4)/GCE对腺嘌呤和鸟嘌呤表现出明显的不可逆氧化峰。峰电流与分析物浓度在0.01 ~ 300 μM范围内呈良好的线性关系，检测限分别为66.54和9.1 nm，定量限分别为221.80和30.23 nm。该传感器成功地用于检测尿液、临床血清和DNA提取样品中的腺嘌呤和鸟嘌呤，在这些样品中的峰值回收率达到96 - 104%。干扰恢复结果显示，误差率小于6%，与现有技术相比，该方法具有更低的检测限和更宽的动态范围。这些发现强调了所提出的方法在具有复杂基质的各种真实样品中准确测定腺嘌呤和鸟嘌呤的潜在实用性。

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引用次数: 0

Emerging trends in Optofluidic biosensing: Techniques, applications, and future directions 光流体生物传感的新趋势：技术、应用和未来方向

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-13 DOI: 10.1016/j.biosx.2025.100602

Renu Poria , Sahil Kumar , Deepak Kala , Maciej Sakowicz , Hardeep Tuli , Krishna Kattel , Ankur Kaushal , Shagun Gupta , Deepak Kumar

This review explores the potential of optofluidic biosensing platforms, emphasizing their application in enhancing biosensing capabilities. This paper reports the various platforms, starting with surface plasmon resonance (SPR)-based optofluidic biosensors, covering angular and spectral SPR biosensing, local SPR biosensing, and the sensitivity and limit of detection (LOD) of both propagating and localized SPR, including multiplexing SPR biosensing techniques. This review further examines whispering gallery mode (WGM) microcavity-based biosensors and photonic crystal-based optofluidic biosensors. A comprehensive overview of the fabrication techniques for optofluidic biosensors is provided, detailing strategies such as direct manufacturing and mold replication. Key components such as fluid control, light manipulation, and signal transduction are highlighted. The material choices for optofluidics are discussed, emphasizing their role in biosensing applications. This paper concludes by exploring optofluidic sensing applications, categorized into absorption-based, plasmonic-based, and scattering-based biosensing techniques. Recent advancements and future directions in the field are discussed, highlighting the integration of these technologies into portable, multiplexed platforms for broader application and ease of use in various scientific and practical fields.

本文综述了光流体生物传感平台的发展潜力，重点介绍了其在提高生物传感能力方面的应用。本文报道了各种平台，从基于表面等离子体共振（SPR）的光流体生物传感器开始，涵盖角度和光谱SPR生物传感，局部SPR生物传感，以及传播和局部SPR的灵敏度和检测限（LOD），包括多路SPR生物传感技术。本文进一步综述了基于窃窃廊模式（WGM）的微腔生物传感器和基于光子晶体的光流体生物传感器。全面概述了光流体生物传感器的制造技术，详细介绍了直接制造和模具复制等策略。重点介绍了流体控制、光操作和信号转导等关键组件。讨论了光流体材料的选择，强调了它们在生物传感应用中的作用。本文最后探讨了光流体传感的应用，分为基于吸收的、基于等离子体的和基于散射的生物传感技术。讨论了该领域的最新进展和未来方向，重点介绍了将这些技术集成到便携式多路复用平台中，以便在各种科学和实践领域中得到更广泛的应用和易用性。

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