Biosensors and Bioelectronics: X最新文献

英文中文

Sensitive and selective electrochemical biosensor based on Manganese(II) complex for simultaneous determination of adenine and guanine from clinical samples and DNA extract samples

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-14 DOI: 10.1016/j.biosx.2025.100603

Melaku Metto , Alemu Tesfaye , Minaleshewa Atlabachew , Abayneh Munshea , Atakilt Abebe

The purines guanine and adenine are essential building blocks for nucleic acids and influence numerous biochemical processes in organisms. Elevated levels of these compounds in the bloodstream can indicate conditions such as cancer and provide insights into cellular energy status, tissue degradation, and enzyme malfunctions in metabolic pathways.

This study focuses on developing a voltammetric sensor through the electropolymerization of a tetraresorcinatemanganate (II) complex on a glassy carbon electrode (poly(Mn(HR)₄)/GCE), which was thoroughly characterized. The poly(Mn(HR)₄)/GCE exhibited distinct, well-defined irreversible oxidative peaks for adenine and guanine. The peak currents displayed strong linearity with analyte concentrations within the 0.01–300 μM range, boasting a detection limit of 66.54 and 9.1 nm and a limit of quantifications of 221.80 and 30.23 nm, respectively. The sensor was successfully employed to detect adenine and guanine in urine, clinical blood serum, and DNA extract samples, with spike recovery rates in these samples reaching the range of 96–104 %. The interference recovery results showcased an error rate of less than 6 %, highlighting the method's superior lower detection limit and broader dynamic range compared to existing techniques. These findings underscore the potential practicality of the proposed approach for accurately determining adenine and guanine in diverse real samples with intricate matrices.

{"title":"Sensitive and selective electrochemical biosensor based on Manganese(II) complex for simultaneous determination of adenine and guanine from clinical samples and DNA extract samples","authors":"Melaku Metto , Alemu Tesfaye , Minaleshewa Atlabachew , Abayneh Munshea , Atakilt Abebe","doi":"10.1016/j.biosx.2025.100603","DOIUrl":"10.1016/j.biosx.2025.100603","url":null,"abstract":"<div><div>The purines guanine and adenine are essential building blocks for nucleic acids and influence numerous biochemical processes in organisms. Elevated levels of these compounds in the bloodstream can indicate conditions such as cancer and provide insights into cellular energy status, tissue degradation, and enzyme malfunctions in metabolic pathways.</div><div>This study focuses on developing a voltammetric sensor through the electropolymerization of a tetraresorcinatemanganate (II) complex on a glassy carbon electrode (poly(Mn(HR)<sub>4</sub>)/GCE), which was thoroughly characterized. The poly(Mn(HR)<sub>4</sub>)/GCE exhibited distinct, well-defined irreversible oxidative peaks for adenine and guanine. The peak currents displayed strong linearity with analyte concentrations within the 0.01–300 μM range, boasting a detection limit of 66.54 and 9.1 nm and a limit of quantifications of 221.80 and 30.23 nm, respectively. The sensor was successfully employed to detect adenine and guanine in urine, clinical blood serum, and DNA extract samples, with spike recovery rates in these samples reaching the range of 96–104 %. The interference recovery results showcased an error rate of less than 6 %, highlighting the method's superior lower detection limit and broader dynamic range compared to existing techniques. These findings underscore the potential practicality of the proposed approach for accurately determining adenine and guanine in diverse real samples with intricate matrices.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100603"},"PeriodicalIF":10.61,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging trends in Optofluidic biosensing: Techniques, applications, and future directions

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-13 DOI: 10.1016/j.biosx.2025.100602

Renu Poria , Sahil Kumar , Deepak Kala , Maciej Sakowicz , Hardeep Tuli , Krishna Kattel , Ankur Kaushal , Shagun Gupta , Deepak Kumar

This review explores the potential of optofluidic biosensing platforms, emphasizing their application in enhancing biosensing capabilities. This paper reports the various platforms, starting with surface plasmon resonance (SPR)-based optofluidic biosensors, covering angular and spectral SPR biosensing, local SPR biosensing, and the sensitivity and limit of detection (LOD) of both propagating and localized SPR, including multiplexing SPR biosensing techniques. This review further examines whispering gallery mode (WGM) microcavity-based biosensors and photonic crystal-based optofluidic biosensors. A comprehensive overview of the fabrication techniques for optofluidic biosensors is provided, detailing strategies such as direct manufacturing and mold replication. Key components such as fluid control, light manipulation, and signal transduction are highlighted. The material choices for optofluidics are discussed, emphasizing their role in biosensing applications. This paper concludes by exploring optofluidic sensing applications, categorized into absorption-based, plasmonic-based, and scattering-based biosensing techniques. Recent advancements and future directions in the field are discussed, highlighting the integration of these technologies into portable, multiplexed platforms for broader application and ease of use in various scientific and practical fields.

{"title":"Emerging trends in Optofluidic biosensing: Techniques, applications, and future directions","authors":"Renu Poria , Sahil Kumar , Deepak Kala , Maciej Sakowicz , Hardeep Tuli , Krishna Kattel , Ankur Kaushal , Shagun Gupta , Deepak Kumar","doi":"10.1016/j.biosx.2025.100602","DOIUrl":"10.1016/j.biosx.2025.100602","url":null,"abstract":"<div><div>This review explores the potential of optofluidic biosensing platforms, emphasizing their application in enhancing biosensing capabilities. This paper reports the various platforms, starting with surface plasmon resonance (SPR)-based optofluidic biosensors, covering angular and spectral SPR biosensing, local SPR biosensing, and the sensitivity and limit of detection (LOD) of both propagating and localized SPR, including multiplexing SPR biosensing techniques. This review further examines whispering gallery mode (WGM) microcavity-based biosensors and photonic crystal-based optofluidic biosensors. A comprehensive overview of the fabrication techniques for optofluidic biosensors is provided, detailing strategies such as direct manufacturing and mold replication. Key components such as fluid control, light manipulation, and signal transduction are highlighted. The material choices for optofluidics are discussed, emphasizing their role in biosensing applications. This paper concludes by exploring optofluidic sensing applications, categorized into absorption-based, plasmonic-based, and scattering-based biosensing techniques. Recent advancements and future directions in the field are discussed, highlighting the integration of these technologies into portable, multiplexed platforms for broader application and ease of use in various scientific and practical fields.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100602"},"PeriodicalIF":10.61,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high performance laser induced graphene (LIG) dual biosensor for simultaneous monitoring of glucose and lactate

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-12 DOI: 10.1016/j.biosx.2025.100600

Hassan Hamidi , Richard Murray , Vincenzo Vezzoni , Somayyeh Bozorgzadeh , Alan O'Riordan , Daniele Pontiroli , Mauro Riccò , Aidan J. Quinn , Daniela Iacopino

The growing demand for efficient and reliable biosensors has driven significant advancements in the development of innovative platforms for real-time monitoring of key biomarkers. In this work, we developed a novel dual biosensing platform designed for simultaneous detection of glucose and lactate, leveraging a laser scribing technology. Individual glucose and lactate biosensors were firstly fabricated, using three electrode designs and Laser Induced Graphene (LIG) as transduction electrode. The individual biosensors displayed high linear range in the relevant sweat physiological range, good sensitivity (41.1 and 12.4 μA mM⁻¹ cm⁻² for glucose and lactate, respectively), Limit of Detection (LOD, 14.9 μM and 2.4 mM for glucose and lactate) and superior short- and long-term stability. Following optimization and in-depth characterization of the individual platforms, a dual sensing platform was designed to offer robust and reliable simultaneous detection of glucose and lactate in a single, integrated system. The dual biosensor maintained the performance displayed in single format, exhibiting sensitivities of 41.2 μA cm⁻² mM⁻¹ and 12.0 μA cm⁻² mM⁻¹ for glucose and lactate, respectively. The dual platform was successfully tested in artificial sweat, demonstrating its potential for precise and reliable biomarker monitoring in continuous health monitoring systems and point-of-care diagnostic.

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引用次数: 0

Modeling of a BK7 prism/Ag/Ni/ZnSe/SM-based sensor for maintaining optimum performance in glucose level detection

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-11 DOI: 10.1016/j.biosx.2025.100604

Kazi Mustafizur Rahman

Patients suffering from diabetes, or having problems regarding up-downs in glucose levels may need to prick human skin to draw blood samples for checkups. Due to the enhancement of photonics, surface plasmon resonance (SPR) is a relief to them. As of now, glucose levels can be detected from urine instead of blood. This research delves into the characterization of an SPR sensor combining a prism-coupled Silver (Ag), Nickel (Ni), and Zinc Selenide (ZnSe) hybrid modeled structure to maintain overall enhanced performance for the detection of glucose concentrations of the patients in urine samples. The motivation is to develop an optimum resulting surface plasmon resonance sensor for this issue based on the Kretschmann configuration. In the present work, the standard transfer matrix method and the Fresnel formulations were utilized to investigate this study. The proposed biosensor comprises nickel (Ni) and zinc selenide (ZnSe), which improves the sensitivity of the surface plasmon resonance-based sensor and protects Ag from being oxidized. The findings reveal high-overall performance parameters are a sensitivity of 329.21°/RIU, full width at half maximum of 3.89 (degrees), a figure of merit of 84.489398, a quality factor of 84.575835 (1/RIU), and a detection accuracy of 0.2570694 (1/degree) for the BK7 prism/Ag/Ni/ZnSe/sensing medium configuration at a wavelength of 633 nm. The exalted performance of this biosensor is efficient in detecting the concentration level of different liquid analytes in biosensing platforms, offering significant promise for the analysis of glucose concentration.

{"title":"Modeling of a BK7 prism/Ag/Ni/ZnSe/SM-based sensor for maintaining optimum performance in glucose level detection","authors":"Kazi Mustafizur Rahman","doi":"10.1016/j.biosx.2025.100604","DOIUrl":"10.1016/j.biosx.2025.100604","url":null,"abstract":"<div><div>Patients suffering from diabetes, or having problems regarding up-downs in glucose levels may need to prick human skin to draw blood samples for checkups. Due to the enhancement of photonics, surface plasmon resonance (SPR) is a relief to them. As of now, glucose levels can be detected from urine instead of blood. This research delves into the characterization of an SPR sensor combining a prism-coupled Silver (Ag), Nickel (Ni), and Zinc Selenide (ZnSe) hybrid modeled structure to maintain overall enhanced performance for the detection of glucose concentrations of the patients in urine samples. The motivation is to develop an optimum resulting surface plasmon resonance sensor for this issue based on the Kretschmann configuration. In the present work, the standard transfer matrix method and the Fresnel formulations were utilized to investigate this study. The proposed biosensor comprises nickel (Ni) and zinc selenide (ZnSe), which improves the sensitivity of the surface plasmon resonance-based sensor and protects Ag from being oxidized. The findings reveal high-overall performance parameters are a sensitivity of 329.21°/RIU, full width at half maximum of 3.89 (degrees), a figure of merit of 84.489398, a quality factor of 84.575835 (1/RIU), and a detection accuracy of 0.2570694 (1/degree) for the BK7 prism/Ag/Ni/ZnSe/sensing medium configuration at a wavelength of 633 nm. The exalted performance of this biosensor is efficient in detecting the concentration level of different liquid analytes in biosensing platforms, offering significant promise for the analysis of glucose concentration.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100604"},"PeriodicalIF":10.61,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cereals as sources of lysine in the reformulation of meat products. Evaluation using a biosensor

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-03 DOI: 10.1016/j.biosx.2025.100592

Erika Alvarez Cañarte , Guilber Vergara Velez , Frank Guillermo Intriago Flor , Efrain Pérez Vega , Miguel Andrès Falconi Vèlez , Delia Noriega Verdugo , Génesis Pamela García García , Livis Sharith Díaz Alarcón , Andrés Miguel Anchundia Loor , Carlos Jadán-Piedra , Felipe Jadán Piedra

The energy and protein requirements of the population must be met, and the use of new analytical methods for rapid, low-cost detection of essential elements like lysine in reformulated foods is crucial. In this context, conditions were evaluated to develop a biosensor with lysine alpha oxidase (LOx), which showed high affinity for lysine with a K_M of 0.32 mM. Different concentrations of cereals and legumes (70-30; 55-45; 85-15; quinoa-Lablab Purpureus; pole beans-Lablab Purpureus; and rye-Lablab Purpureus) were incorporated into meat sausages to enrich lysine, achieving a significant increase in lysine concentration (up to 75%) when 15% quinoa was substituted. The potentiometric signal, related to oxygen consumption during lysine oxidation, was detected at 15 s using a voltage of −600 mV. The biosensor, coupled with the immobilized enzyme, allowed the use of low volumes. A positive relationship was found between oxygen consumption (mg O₂/L∗s-1) and lysine concentration in the range of 0.01–0.2 mM, with an R² of 0.9964. The immobilized enzyme-based sensor demonstrated good sensitivity (0.01 mM) and the membrane could be reused up to 18 times, maintaining 92% of its initial activity after 70 days. The biosensor method showed minimal residue formation and had a strong correlation with high-performance liquid chromatography (HPLC) results, validating its accuracy.

{"title":"Cereals as sources of lysine in the reformulation of meat products. Evaluation using a biosensor","authors":"Erika Alvarez Cañarte , Guilber Vergara Velez , Frank Guillermo Intriago Flor , Efrain Pérez Vega , Miguel Andrès Falconi Vèlez , Delia Noriega Verdugo , Génesis Pamela García García , Livis Sharith Díaz Alarcón , Andrés Miguel Anchundia Loor , Carlos Jadán-Piedra , Felipe Jadán Piedra","doi":"10.1016/j.biosx.2025.100592","DOIUrl":"10.1016/j.biosx.2025.100592","url":null,"abstract":"<div><div>The energy and protein requirements of the population must be met, and the use of new analytical methods for rapid, low-cost detection of essential elements like lysine in reformulated foods is crucial. In this context, conditions were evaluated to develop a biosensor with lysine alpha oxidase (LOx), which showed high affinity for lysine with a K<sub>M</sub> of 0.32 mM. Different concentrations of cereals and legumes (70-30; 55-45; 85-15; quinoa-Lablab Purpureus; pole beans-Lablab Purpureus; and rye-Lablab Purpureus) were incorporated into meat sausages to enrich lysine, achieving a significant increase in lysine concentration (up to 75%) when 15% quinoa was substituted. The potentiometric signal, related to oxygen consumption during lysine oxidation, was detected at 15 s using a voltage of −600 mV. The biosensor, coupled with the immobilized enzyme, allowed the use of low volumes. A positive relationship was found between oxygen consumption (mg O<sub>2</sub>/L∗s-1) and lysine concentration in the range of 0.01–0.2 mM, with an R<sup>2</sup> of 0.9964. The immobilized enzyme-based sensor demonstrated good sensitivity (0.01 mM) and the membrane could be reused up to 18 times, maintaining 92% of its initial activity after 70 days. The biosensor method showed minimal residue formation and had a strong correlation with high-performance liquid chromatography (HPLC) results, validating its accuracy.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100592"},"PeriodicalIF":10.61,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Multiple and simultaneous detection for cytokines based on the nanohole array by electrochemical sandwich immunoassay” [Biosens Bioelectron.: X 14 (2023) 100387]

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-02-27 DOI: 10.1016/j.biosx.2025.100598

Han Na Suh , Sung-Hoon Yoon , Yoo Min Park , HyungJin Kim , SangHee Kim , JuKyung Lee

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy).

This article has been retracted at the request of author JuKyung Lee, with approval from Biosensors and Bioelectronics: X editor Dianping Tang.

Concerns were raised following the publication of this paper about improper use of the data and discrepancies and misinterpretations of the data. The design presented in the papers were obtained at Northeasten University, the data have been published without permission.

Authors Han Na Suh; Sung-Hoon Yoon; Yoo Min Park; HyungJin Kim; and SangHee Kim have been notified of the retraction.

Based on the evidence, the above-mentioned authors and the editor concluded the article should be retracted.

引用次数: 0

Conductive polymer composites in soft robotics, flexible sensors and energy storage: Fabrication, applications and challenges

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-02-25 DOI: 10.1016/j.biosx.2025.100597

Hassan Tawsif Tazwar , Maisha Farzana Antora , Itmam Nowroj , Adib Bin Rashid

Conductive Polymer Composites (CPCs) have emerged as promising materials with applications in soft robotics, flexible sensors, and energy storage. This review paper begins with a brief introduction to the available polymers and additives in context of CPCs, followed by a classification of the resulting composites, setting the stage for further discussions. Then we sequentially analyze the fabrication techniques and practical applications of CPC in the aforementioned sectors highlighting their performance. Furthermore, the review delves into critical aspects such as failure modes, including interfacial debonding and fatigue, the environmental impact and sustainability of CPCs, considering the source and biodegradability of polymers and the environmental implications of additives. Limitations such as particle leakage in metal-based flexible devices, nanoparticle agglomeration and high cost of modern fabrication techniques have been discussed. Future research directions, including development of cost-effective manufacturing methods and proper encapsulation techniques for flexible devices have been highlighted.

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引用次数: 0

Characterization of plant pathogenic bacteria at subspecies level using a dielectrophoresis device combined with Raman spectroscopy

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100595

Alessio Sacco , Camilla Sacco Botto , Chiara D'Errico , Marina Ciuffo , Slavica Matić , Giulia Molinatto , Andrea M. Giovannozzi , Andrea M. Rossi , Emanuela Noris

Timely diagnosis of plant diseases and correct identification of etiological agents are fundamental to guarantee quality and quantity of agricultural products and food. Phytopathogenic bacteria induce devastating effects on crops. Their diagnosis and identification, mainly based on serological and molecular tools, are time consuming and expensive processes and require trained personnel. Among the innovative methods providing rapid, accurate, and reliable diagnosis at reduced costs, Raman spectroscopy (RS) is gathering considerable attention. RS provides a direct and non-destructive platform to gather information on the chemical and biochemical components of a sample, such as microorganism cultures, revealing their biological role. Due to the weak signals of bacterial cells in RS, a dielectrophoresis (DEP) approach was adopted to amplify the bacterial signals. Using Raman-DEP analysis, a dataset of spectra from different harmful phytopathogenic bacteria belonging to the genera Pseudomonas spp., Xanthomonas spp., and Erwinia spp. was obtained. Machine learning approaches were employed to discriminate isolates at the genus, species, and unprecedentedly at the pathovar level, reaching accuracies, precisions, recalls, and F1 scores of 94–100%. This approach offers important advancements in the non-destructive and rapid classification of microorganisms and is suitable to be readily extended to environmental and food diagnostics.

{"title":"Characterization of plant pathogenic bacteria at subspecies level using a dielectrophoresis device combined with Raman spectroscopy","authors":"Alessio Sacco , Camilla Sacco Botto , Chiara D'Errico , Marina Ciuffo , Slavica Matić , Giulia Molinatto , Andrea M. Giovannozzi , Andrea M. Rossi , Emanuela Noris","doi":"10.1016/j.biosx.2025.100595","DOIUrl":"10.1016/j.biosx.2025.100595","url":null,"abstract":"<div><div>Timely diagnosis of plant diseases and correct identification of etiological agents are fundamental to guarantee quality and quantity of agricultural products and food. Phytopathogenic bacteria induce devastating effects on crops. Their diagnosis and identification, mainly based on serological and molecular tools, are time consuming and expensive processes and require trained personnel. Among the innovative methods providing rapid, accurate, and reliable diagnosis at reduced costs, Raman spectroscopy (RS) is gathering considerable attention. RS provides a direct and non-destructive platform to gather information on the chemical and biochemical components of a sample, such as microorganism cultures, revealing their biological role. Due to the weak signals of bacterial cells in RS, a dielectrophoresis (DEP) approach was adopted to amplify the bacterial signals. Using Raman-DEP analysis, a dataset of spectra from different harmful phytopathogenic bacteria belonging to the genera <em>Pseudomonas</em> spp., <em>Xanthomonas</em> spp., and <em>Erwinia</em> spp. was obtained. Machine learning approaches were employed to discriminate isolates at the genus, species, and unprecedentedly at the pathovar level, reaching accuracies, precisions, recalls, and F1 scores of 94–100%. This approach offers important advancements in the non-destructive and rapid classification of microorganisms and is suitable to be readily extended to environmental and food diagnostics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100595"},"PeriodicalIF":10.61,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and application of enzyme-based hydrogels

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100594

Yahui Wen , Xinghai Wang , Jinxue Zhao , Xuejing Zhai , Wei Xia , Peiyi Li , Keqiang Lai , Lidong Wu

Enzymes are highly efficient catalysts in nature, governing countless reactions in biological systems. Enzyme-based hydrogels are three-dimensional network materials with enzyme activity, created by loading enzymes into hydrogels or using hydrogels to synthesize polymeric materials. Due to their high biocompatibility, enzyme-based hydrogels exhibit broad application potential in various fields. In recent years, researchers have been continuously exploring new preparation methods to improve the physical, chemical, and biological properties of enzyme-based hydrogels. Additionally, the introduction of materials such as graphene, carbon nanotubes, and metal-organic frameworks (MOFs) has further enhanced the biological activity of these hydrogels. This review summarizes recent preparation methods and structural characteristics of enzyme-based hydrogels, with a focus on their applications in biosensors and medical fields, highlighting the vast application prospects of enzyme-based hydrogels.

引用次数: 0

Nanostructured immunosensing system for label-free impedimetric detection of multiple breast cancer biomarkers (CEA and HER2) using CoMoO4@PANI-PPy Nanocomposite

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100596

Omar Ramadan, Rabeay Y.A. Hassan

Breast cancer patients often exhibit positive expression of selective tumor biomarkers including the carcinoembryonic antigen (CEA), and human epidermal growth factor receptor 2 (HER2). These potential protein biomarkers are highly crucial for the selective and early tumor diagnosis. In this study, disposable dual impedimetric immunosensing systems were constructed using the cobalt molybdate (CoMoO₄) and polyaniline/polypyrrole (PANI-PPy) nanocomposite, for the rapid quantification of CEA and HER2 concentration levels. The designed sensing probes were fabricated by chemically conjugating two-targeting antibodies (anti-CEA and anti-HER2) on the nanostructured electrode surfaces modified with (AuNPs/CoMoO₄@PANI-PPy). These successful chemical antibody immobilizations were enabled by the use of 4-aminothiophenol (4-ATP) as a cross-linking agent. The impedimetric immunosensing assay was fully optimized by screening of nanomaterials, determining the optimal ratio of the nanocomposite, adjusting the time of self-assembled monolayer (SAM) formation, and identifying the degree of cross-reactivity with potential non-targeting molecules. Accordingly, the biosensors demonstrated distinguished linear dynamic ranges of 1.0 fg/mL to 100 ng/mL for the CEA and 25 fg/mL to 100 ng/mL for the HER2, with detection limits of 8.2 fg/mL and 4.9 fg/mL for CEA and HER2, respectively. The immunesensor's reproducibility and stability were studied, showing minimal variation in charge transfer resistance (RSD = 1.33%, n = 5) and maintaining 82.5% functionality after four weeks of storage at 4 °C. These findings highlighted the potential use of these biosensor chips for clinical applications in early breast cancer detection and rapid diagnosis.

乳腺癌患者通常表现出选择性肿瘤生物标记物的阳性表达，包括癌胚抗原（CEA）和人类表皮生长因子受体 2（HER2）。这些潜在的蛋白质生物标志物对于选择性早期肿瘤诊断至关重要。本研究利用钼酸钴（CoMoO4）和聚苯胺/聚吡咯（PANI-PPy）纳米复合材料构建了一次性双阻抗免疫传感系统，用于快速量化 CEA 和 HER2 的浓度水平。所设计的传感探针是通过化学方法将两种靶向抗体（抗 CEA 和抗 HER2）缀合在经（AuNPs/CoMoO4@PANI-PPy）修饰的纳米结构电极表面而制成的。使用 4- 氨基苯硫酚（4-ATP）作为交联剂成功实现了化学抗体固定。通过筛选纳米材料、确定纳米复合材料的最佳比例、调整自组装单层（SAM）的形成时间以及确定与潜在非靶向分子的交叉反应程度，对阻抗免疫传感测定进行了全面优化。因此，生物传感器显示出卓越的线性动态范围：CEA 为 1.0 fg/mL 至 100 ng/mL，HER2 为 25 fg/mL 至 100 ng/mL，CEA 和 HER2 的检测限分别为 8.2 fg/mL 和 4.9 fg/mL。对免疫传感器的再现性和稳定性进行了研究，结果显示电荷转移电阻的变化极小（RSD = 1.33%，n = 5），在 4 °C 下储存四周后仍能保持 82.5% 的功能。这些研究结果突显了这些生物传感器芯片在早期乳腺癌检测和快速诊断中的临床应用潜力。

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