Biosensors and Bioelectronics: X最新文献_第4页

Targeted biosensors for intracellular lipid droplet content detection 用于检测细胞内脂滴含量的靶向生物传感器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-10 DOI: 10.1016/j.biosx.2024.100551

Shengdong Li

Lipid droplets participate in the metabolic process of many living cells, and their abnormalities are highly correlated with many diseases such as fatty liver, diabetes, and cancer. Nevertheless, the current methods are inadequate for elucidating the relationship between diverse diseases and lipid droplets. In particular, the visualization of tumors with abnormal distribution of lipid droplets remains a significant challenge. Therefore, a water-soluble biosensor with lipid droplet specificity is synthesized by copolymerization of coumarin derivatives with low toxicity and high hydrophilic hydroxyl terminal polyethylene glycol. The survival rate of HeLa cells cultured with lipid drop biosensor for 24 h was more than 90% on average, and the toxicity was less. The co-localization imaging experiments with lipid droplets showed that the biosensor had a good ability to target lipid droplets in HeLa cells. The experimental results of the response of different cell lines to the biosensor showed that the tumor cells had a good response and uptake ability. These results indicate that the prepared biosensor has a good distinguishing function between normal cells and tumor cells, and provides an experimental basis for the early diagnosis of clinical tumors.

脂滴参与许多活细胞的新陈代谢过程，其异常与脂肪肝、糖尿病和癌症等多种疾病高度相关。然而，目前的方法还不足以阐明各种疾病与脂滴之间的关系。特别是，如何观察脂滴分布异常的肿瘤仍然是一个重大挑战。因此，通过低毒性香豆素衍生物与高亲水性羟基端聚乙二醇的共聚，合成了一种具有脂滴特异性的水溶性生物传感器。用脂滴生物传感器培养 HeLa 细胞 24 小时的存活率平均超过 90%，且毒性较低。脂滴共定位成像实验表明，该生物传感器能很好地靶向 HeLa 细胞中的脂滴。不同细胞系对生物传感器的反应实验结果表明，肿瘤细胞对生物传感器具有良好的反应和吸收能力。这些结果表明，制备的生物传感器具有良好的区分正常细胞和肿瘤细胞的功能，为临床肿瘤的早期诊断提供了实验依据。

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引用次数: 0

Intact photosynthetic bacteria-based electrodes for self-powered metal ions monitoring 基于完整光合细菌的自供电金属离子监测电极

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-10 DOI: 10.1016/j.biosx.2024.100552

Jefferson Honorio Franco , Paolo Stufano , Rossella Labarile , Dario Lacalamita , Pierluigi Lasala , Elisabetta Fanizza , Massimo Trotta , Gianluca Maria Farinola , Matteo Grattieri

The low-cost and early monitoring of metal ion contaminants is paramount to prevent widespread contamination of water environments. Self-powered microbial electrochemical sensors represent an interesting approach to achieving this goal. Purple non-sulfur bacteria have a versatile metabolism and a well-characterized photosynthetic system, making them an ideal candidate for developing biohybrid technologies. In this work, we report the use of these bacteria in biophotoelectrodes to develop self-powered monitoring systems for two common pollutants, NiCl₂ and CuSO₄. The microbial biophotoelectrode was obtained on a homemade poly-hydroxybutyrate-carbon nanofibers electrode modified with a redox-adhesive polydopamine matrix-based entrapping the purple bacterium Rhodobacter capsulatus. The presence of 500 μM NiCl₂ resulted in a 60 % decrease in current density, while the simultaneous presence of 100 μM NiCl₂ and 100 mM CuSO₄ led to an 83 % current inhibition. Given the implementation of the biophotoelectrode in the field, the biohybrid system was tested in a complex matrix containing beer, demonstrating the promising ability of the photoelectrochemical system to act as an efficient biosensor in complex solutions. Finally, the biohybrid electrode was coupled to a cathode performing oxygen reduction, which allowed obtaining a self-powered monitoring system, paving the way for the future implementation of a low-cost monitoring system for widespread metal ions contaminant monitoring.

低成本和早期监测金属离子污染物对于防止水环境大范围污染至关重要。自供电微生物电化学传感器是实现这一目标的有趣方法。紫色非硫细菌具有多功能的新陈代谢和表征良好的光合作用系统，是开发生物杂交技术的理想候选者。在这项工作中，我们报告了在生物光电电极中使用这些细菌开发自供电监测系统的情况，以监测两种常见污染物--NiCl2 和 CuSO4。微生物生物光电电极是在自制的聚羟基丁酸碳纳米纤维电极上获得的，该电极以氧化还原粘附性聚多巴胺基质为基础进行了修饰，其中夹杂着紫色荚膜罗杆菌。500 μM NiCl2 的存在导致电流密度降低了 60%，而同时存在 100 μM NiCl2 和 100 mM CuSO4 则导致 83% 的电流抑制。考虑到生物光电电极在现场的应用，生物杂交系统在含有啤酒的复杂基质中进行了测试，证明了光电化学系统在复杂溶液中充当高效生物传感器的能力。最后，生物杂化电极与一个进行氧还原的阴极耦合，从而获得了一个自供电的监测系统，为将来实施低成本监测系统以广泛监测金属离子污染物铺平了道路。

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引用次数: 0

A novel approach to tyrosinase-based biosensors: Electrode reactions and biological measurement 基于酪氨酸酶的生物传感器的新方法：电极反应和生物测量

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-05 DOI: 10.1016/j.biosx.2024.100550

Masumeh Goodarzi , Hosna Tavakoli , Maryam Arab Khalaj , Hassan Tavakoli

This study introduces a novel tyrosinase-based biosensor designed to simultaneously detect tyrosinase, tyrosine, L-DOPA, and L-DOPA quinone. The biosensor, designated as GCE/GNP/Cys/Chit/Tyrase, was developed by immobilizing tyrosinase on a modified glassy carbon electrode (GCE) that incorporates electrodeposited gold nanoparticles, cysteine, and chitosan. The morphology of the biosensor was characterized using scanning electron microscopy (SEM). The electrochemical behaviors of the biosensor were explored in response to the target analytes. Key analytical characteristics were assessed, including linear range, sensitivity, selectivity, limits of detection and quantification, long-term stability, repeatability, reproducibility, electrochemically active surface area, and charge transfer behavior. The biosensor demonstrated a linear response range of 1–120 μM, with an impressive sensitivity of 200.4 mA.Lmol⁻¹cm⁻² and a detection limit of 27 μM. This study provides a comprehensive evaluation of the analytical features of tyrosinase-based biosensors. Additionally, the biosensor was applied to quantify dopamine in brain tissue, utilizing a calibration curve derived from the fabricated biosensor. The dopamine concentration measured in five Wistar rats was 35 ± 2.75 μM, reflecting the mean and standard deviation, respectively. These results confirm the biosensor's capability for accurately detecting this critical neurotransmitter in the brains of Wistar rats. This investigation underscores the potential of tyrosinase-based biosensors for diverse analytical applications in biological samples.

本研究介绍了一种基于酪氨酸酶的新型生物传感器，旨在同时检测酪氨酸酶、酪氨酸、L-DOPA 和 L-DOPA 醌。该生物传感器被命名为 GCE/GNP/Cys/Chit/Tyrase，是通过将酪氨酸酶固定在改良的玻璃碳电极（GCE）上而开发出来的，该电极结合了电沉积金纳米粒子、半胱氨酸和壳聚糖。使用扫描电子显微镜（SEM）对生物传感器的形态进行了表征。针对目标分析物，对生物传感器的电化学行为进行了探索。对主要分析特性进行了评估，包括线性范围、灵敏度、选择性、检出限和定量限、长期稳定性、重复性、再现性、电化学活性表面积和电荷转移行为。该生物传感器的线性响应范围为 1-120 μM，灵敏度高达 200.4 mA.Lmol-1cm-2，检测限为 27 μM。这项研究全面评估了基于酪氨酸酶的生物传感器的分析特性。此外，该生物传感器还被用于定量检测脑组织中的多巴胺，使用的校准曲线是由制造的生物传感器得出的。在五只 Wistar 大鼠体内测得的多巴胺浓度为 35 ± 2.75 μM，分别反映了平均值和标准偏差。这些结果证实了该生物传感器能够准确检测 Wistar 大鼠大脑中的这种关键神经递质。这项研究凸显了基于酪氨酸酶的生物传感器在生物样本的多种分析应用中的潜力。

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引用次数: 0

An electroactive microfluidic platform integrated with AM-pDEP focusing and side-counter design for selective cell sorting and single-cell quantification 集成 AM-pDEP 聚焦和侧面计数器设计的电活性微流控平台，用于选择性细胞分拣和单细胞定量

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-05 DOI: 10.1016/j.biosx.2024.100549

Zuyuan Tian , Mohamed Shaheen , Tianxiang Jiang , Shaoxi Wang , Xihua Wang , Jie Chen

Rapid isolation and precise quantification of target cancer cells are crucial for precision medicine applications. Conventional fluorescence-based methods require bulky optical instrumentation and specialized expertise. Recent advances in label-free microfluidic techniques have attempted to overcome these limitations but often suffer from reduced reliability and throughput when integrating multiple functions. Here, we demonstrate an electroactive microfluidic platform that integrates functions of selective concentration, particle focusing, and single-cell level quantification without introducing additional complex physical microstructures. Our device employs the dielectrophoresis (DEP) effect combined with an interdigitated cell sorter to achieve selective cell concentration. For quantification and characterization, the device leverages the impedimetric Coulter principle to achieve precise particle counting and characterization at single-cell resolution. To address the inherent tradeoff between sensitivity and throughput in the traditional Coulter counter, we utilize the tilted interdigitated electrode configuration excited by an amplitude-modulated (AM) Positive DEP (pDEP) signal instead of the commonly employed narrow channel or sheath flow to realize the effective focusing and alignment of the cell stream. The focusing module is combined with our side-counter design to detect cells. The performance of our device and its embedded modules has been verified with mixed breast cancer and blood model cell lines, where high consistency between optical and electrical detection has been demonstrated. We expect the integration of the proposed AM-pDEP focusing approach and the corresponding microfluidic design for cell sorter and on-chip flow cytometry would offer an alternative engineering solution to the stream-based on-chip single-cell analysis where reliable cell focusing and single-cell sensing are required.

目标癌细胞的快速分离和精确定量对于精准医疗应用至关重要。传统的荧光方法需要笨重的光学仪器和专业知识。无标记微流控技术的最新进展试图克服这些限制，但在集成多种功能时往往会降低可靠性和吞吐量。在这里，我们展示了一种电活性微流控平台，它集成了选择性浓缩、粒子聚焦和单细胞水平定量等功能，而无需引入额外的复杂物理微结构。我们的设备采用了介电泳（DEP）效应，并结合了交叉细胞分拣机，实现了细胞的选择性浓缩。在定量和表征方面，该设备利用阻抗库尔特原理，以单细胞分辨率实现精确的粒子计数和表征。为了解决传统库尔特计数器在灵敏度和处理量之间的固有权衡问题，我们利用由调幅（AM）正向电泳（pDEP）信号激发的倾斜交互电极配置，而不是通常采用的窄通道或鞘流来实现细胞流的有效聚焦和对齐。聚焦模块与我们的侧面计数器设计相结合，用于检测细胞。我们的设备及其嵌入式模块的性能已在混合乳腺癌和血液模型细胞系中得到验证，证明了光学和电学检测之间的高度一致性。我们希望将所提出的 AM-pDEP 聚焦方法和相应的微流体设计整合到细胞分拣机和片上流式细胞仪中，为需要可靠的细胞聚焦和单细胞传感的基于流的片上单细胞分析提供另一种工程解决方案。

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引用次数: 0

Ion-selective electrode based on polyurethane-immobilized di-(2-ethyl hexyl) phosphoric acid for low-concentration aqueous Pb2+ detection and quantification 基于聚氨酯固定化二（2-乙基己基）磷酸的离子选择性电极，用于低浓度水体 Pb2+ 的检测和定量

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-28 DOI: 10.1016/j.biosx.2024.100547

Khairun Nisah , Eka Safitri , Rahmi Rahmi , Muliadi Ramli , Reni Silvia Nasution , Muhammad Iqhrammullah

This study used a polyurethane (PU)-based ion selective electrode (ISE) immobilized with di-(2-ethyl hexyl) phosphoric acid (D2EHPA) to detect and quantify Pb²⁺ ions at low concentrations (10⁻¹⁰ to 10⁻¹ M) in aqueous solution. PU, synthesized from castor oil (Ricinus communis L.), was utilized as the ISE membrane matrix. The ISE demonstrated a sensitivity of 26.24 ± 0.12 mV/decade, a detection limit of 1.44 × 10⁻⁷ M, and a response time of 10 s. We maintained the measurement stability for up to six days of storage.

The results of Pb²⁺ quantification produced by ISE were compared with the results using atomic absorption spectroscopy (AAS), indicating similar Pb²⁺ concentrations in both artificial and real wastewater samples (calculated t-value < theoretical t-value). Therefore, this ISE is suitable for detecting trace levels of Pb²⁺ and quantifying them with high accuracy.

本研究使用固定了二-（2-乙基己基）磷酸（D2EHPA）的聚氨酯（PU）基离子选择电极（ISE）来检测和定量水溶液中低浓度（10-10 至 10-1 M）的 Pb2+ 离子。用蓖麻油（Ricinus communis L.）合成的聚氨酯作为 ISE 膜基质。ISE 的灵敏度为 26.24 ± 0.12 mV/decade，检测限为 1.44 × 10-7 M，响应时间为 10 s。我们将 ISE 的 Pb2+ 定量结果与原子吸收光谱法（AAS）的结果进行了比较，结果表明人工废水和实际废水样品中的 Pb2+ 浓度相似（计算 t 值 < 理论 t 值）。因此，这种 ISE 适用于检测痕量 Pb2+，并能准确定量。

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引用次数: 0

Resting-state brain network analysis and applied evaluation of global developmental delay in preterm infants: A functional near-infrared spectroscopic study 早产儿整体发育迟缓的静息态脑网络分析和应用评估：功能性近红外光谱研究

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-24 DOI: 10.1016/j.biosx.2024.100546

Hengye Huang , Karol Kexin Sun , Dan Wu , Chuncao Zhang , Yanyan Huo , Yuan Tian , Guangjun Yu

Global developmental delay (GDD) is a common neurodevelopmental disorder in children under 5 years of age. This study assessed the potential of functional Near-Infrared Spectroscopy (fNIRS) technology aiding diagnosis of GDD in premature infants and explored brain pathogenesis of GDD. This prospective cohort study was conducted between December 2020 and June 2023 among preterm infants in five hospitals in China. The primary outcome was the diagnosis of GDD. A 5-min fNIRS brain scan was performed in preterm infants at 4-, 8-, and 12-month corrected age in two hospitals. A diagnostic model based on fNIRS brain network and an integrated diagnostic model built on fNIRS data as well as birth head circumference was developed. Diagnostic models were assessed using the area under the curve (AUC) from receiver operating characteristic curves. Brain network analysis revealed significantly worse left-sided functional connectivity in GDD preterm infants. The fNIRS technique had satisfactory diagnostic value for GDD in all age groups. The diagnostic accuracy of the combined diagnostic model significantly improved. This study suggested that fNIRS could be considered for early screening and late auxiliary diagnosis of GDD in preterm infants. Furthermore, it can also be used to explore GDD brain pathogenesis.

全面发育迟缓（GDD）是 5 岁以下儿童常见的神经发育障碍。本研究评估了功能性近红外光谱（fNIRS）技术辅助诊断早产儿全球发育迟缓的潜力，并探讨了全球发育迟缓的脑部发病机制。这项前瞻性队列研究于 2020 年 12 月至 2023 年 6 月在中国五家医院的早产儿中开展。研究的主要结果是GDD的诊断。两家医院分别对4个月、8个月和12个月的早产儿进行了5分钟fNIRS脑部扫描。开发了基于 fNIRS 脑网络的诊断模型和基于 fNIRS 数据及出生头围的综合诊断模型。诊断模型采用接收器操作特征曲线的曲线下面积（AUC）进行评估。脑网络分析显示，GDD 早产儿的左侧功能连接性明显较差。fNIRS 技术对所有年龄组的 GDD 都具有令人满意的诊断价值。联合诊断模型的诊断准确性明显提高。这项研究表明，fNIRS 可用于早产儿 GDD 的早期筛查和后期辅助诊断。此外，它还可用于探索 GDD 脑部发病机制。

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引用次数: 0

Recent advancements in nanozyme hydrogel based system for enhanced sensing applications 基于纳米酶的水凝胶系统在增强传感应用方面的最新进展

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-24 DOI: 10.1016/j.biosx.2024.100548

Simran Makkar , Nikita Sarawagi , Nitesh Priyadarshi , Sunaina Kaul , Palakjot Kour Sodhi , Ajay Kumar Srivastava , Nitin Kumar Singhal

In recent years, combining hydrogels with nanozymes has shown promise as a platform for sensing applications. Nanozymes are simple, affordable, and stable alternatives that use enzyme-like capabilities. Conversely, hydrogels provide hydrated, biocompatible environments, which enhance the stability and activity of nanozymes. The most recent advancements in nanozyme-hydrogel hybrid sensing systems are examined in this study, with a focus on their enzymatic activities—peroxidase and oxidase mimetics—and how they can be used to detect a variety of analytes, such as glucose, pesticide, and many more.

In addition to simulating the biological environment, the synergistic combination of hydrogels and nanozyme improves the biosensor's sensitivity, specificity, and fast reaction times. These hybrid systems also provide an extensible framework for integrating multiple sensory modalities into a single functional device. This review highlights the groundbreaking potential and uses of hydrogel sensors based on nanozymes in environmental and medical diagnostics and potential future developments.

近年来，水凝胶与纳米酶的结合已显示出作为传感应用平台的前景。纳米酶是一种简单、经济、稳定的替代品，具有类似酶的功能。相反，水凝胶提供了水合的、生物兼容的环境，从而提高了纳米酶的稳定性和活性。本研究探讨了纳米酶-水凝胶混合传感系统的最新进展，重点是它们的酶活性--过氧化物酶和氧化酶模拟物--以及它们如何用于检测葡萄糖、农药等多种分析物。这些混合系统还提供了一个可扩展的框架，可将多种感官模式集成到单一功能装置中。本综述重点介绍了基于纳米酶的水凝胶传感器在环境和医疗诊断中的突破性潜力和用途，以及未来的潜在发展。

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引用次数: 0

Smartphone-enhanced nanozyme sensors: Colorimetric and fluorescence sensing techniques 智能手机增强型纳米酶传感器：比色和荧光传感技术

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-19 DOI: 10.1016/j.biosx.2024.100544

Tileshwar Sahare, Nandini Singh, Badri Narayana Sahoo, Abhijeet Joshi

The advent of smartphone technology has changed the biosensing field, especially by integrating nanozyme-based colorimetric, fluorescence sensing strategy. This review article describes the development of smartphone-assisted nanozyme sensor technology, harboring increased sensitivity and specificity for detecting relevant markers. While mimicking natural catalytic enzymes, nanozymes show exceptional performance in a variety of analytical applications. Combining the computing power and imaging capabilities of smartphones, nanozyme based sensors offer a low-cost, user-friendly, and scalable solution for point-of-care technology. We have discussed the principles behind colorimetric and fluorescence sensing techniques, the role of nanozymes in these processes, and the methodologies for leveraging smartphone technology for data acquisition and analysis. Crucial applications in medical diagnostics, environmental monitoring, and food safety are reviewed, with a focus on recent innovations and forthcoming prospects. The convergence of nanozyme sensors and smartphone technology promises to democratize access to advanced colorimetric or fluorometric based biosensing.

智能手机技术的出现改变了生物传感领域，特别是通过整合基于纳米酶的比色、荧光传感策略。这篇综述文章介绍了智能手机辅助纳米酶传感器技术的发展，该技术提高了检测相关标记物的灵敏度和特异性。在模仿天然催化酶的同时，纳米酶在各种分析应用中表现出卓越的性能。结合智能手机的计算能力和成像能力，基于纳米酶的传感器为护理点技术提供了一种低成本、用户友好和可扩展的解决方案。我们讨论了比色和荧光传感技术背后的原理、纳米酶在这些过程中的作用，以及利用智能手机技术进行数据采集和分析的方法。我们回顾了在医疗诊断、环境监测和食品安全方面的重要应用，重点介绍了近期的创新和即将到来的前景。纳米酶传感器和智能手机技术的融合有望使先进的比色法或荧光法生物传感技术平民化。

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引用次数: 0

Advances in gluten detection: A rapid colorimetric approach using core-satellite magnetic particles 麸质检测方面的进展：使用核心卫星磁性颗粒的快速比色法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-17 DOI: 10.1016/j.biosx.2024.100545

Daniele Marra , Adriano Acunzo , Andrea Fulgione , Maria De Luca , Reynaldo Villalonga , Francesco Pisani , Loredana Biondi , Federico Capuano , Raffaele Velotta , Bartolomeo Della Ventura , Vincenzo Iannotti

Monitoring gluten levels in foods labelled as gluten-free or low-gluten — i.e. containing less than 20 ppm and 100 ppm of gluten, respectively — is crucial for preventing celiac disease-related disorders. Due to their inherent complexity, the standard analytical techniques to assess the gluten content in food are not suitable for on-site measurements, whose need is widely recognized. In this context, we developed a rapid and cost-effective biosensor based on core-satellite magnetic particles (CSMPs) — magnetic cores coated with gold nanoparticles (AuNPs) — further functionalized with anti-gliadin antibodies. The study demonstrates that a 0.016% concentration of the surfactant Tween-20 can induce the spontaneous formation of stable CSMP clusters in dispersion. These clusters, composed of weakly interacting functionalized CSMPs, undergo fragmentation in the presence of gliadin, which specifically binds to the antibodies on the CSMPs. This process results in a colour change, which is measurable by a UV–VIS spectrophotometer. Gliadin extraction was achieved by treating the sample with a non-toxic ethanol-water mixture (60%), sufficient to induce a measurable colour change in the presence of gluten contamination, with a limit of detection (LOD) of 8 ppm, which is lower than low limit established for gluten-free food.

监测标注为无麸质或低麸质（即麸质含量分别低于 20 ppm 和 100 ppm）食品中的麸质含量对于预防乳糜泻相关疾病至关重要。由于其固有的复杂性，评估食品中麸质含量的标准分析技术不适合现场测量，而现场测量的需求已得到广泛认可。在这种情况下，我们开发了一种基于磁芯-卫星磁性颗粒（CSMPs）的快速、经济的生物传感器，这种磁性颗粒的磁芯涂有金纳米颗粒（AuNPs），并进一步与抗麸质蛋白抗体功能化。研究表明，0.016% 浓度的表面活性剂吐温-20 可以诱导分散体中自发形成稳定的 CSMP 簇。这些由弱相互作用的官能化 CSMP 组成的团簇在麦胶蛋白存在时会发生碎裂，麦胶蛋白会特异性地与 CSMP 上的抗体结合。这一过程会导致颜色变化，可通过紫外-可见分光光度计测量。用无毒的乙醇-水混合物（60%）处理样品可提取麦胶蛋白，该混合物足以在存在麸质污染的情况下引起可测量的颜色变化，检测限（LOD）为 8 ppm，低于为无麸质食品设定的低限。

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引用次数: 0

Advances in the application of sensor arrays based on nanozymes 基于纳米酶的传感器阵列的应用进展

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-09-11 DOI: 10.1016/j.biosx.2024.100542

Ying Ma, Heng Liu, Bin Li, Na Lu

As efficient biocatalysts, enzymes can catalyze various reactions under mild conditions, and have excellent substrate specificity and selectivity. However, the high cost of preparation and difficulty in recovery of natural enzymes have seriously hindered their practical applications. Compared with natural enzymes, the nanozymes with enzyme-like activities have unique advantages in activity regulation, high stability and large-scale preparation. Nanozymes have been widely concerned in the fields of biological detection, medical treatment and cellular immunity, and have a great application potential in the sensor array field. The sensor arrays have recently received an increasing attention due to their applicability in complex biological analysis and diagnosis. This review first summarizes the classification of nanozymes and their corresponding enzyme-like activities. The applications of nanozymes in various sensor arrays are then introduced, in particular, the detecting small molecule, protein and pesticide as nonspecific recognition receptors are addressed. Finally, the future development directions and design principles are prospected for overcoming current challenges, thus providing more opportunities for the application of nanozyme sensor arrays.

作为高效的生物催化剂，酶可以在温和的条件下催化各种反应，并具有极好的底物特异性和选择性。然而，天然酶的制备成本高、回收困难，严重阻碍了其实际应用。与天然酶相比，具有类酶活性的纳米酶在活性调节、高稳定性和大规模制备等方面具有独特的优势。纳米酶在生物检测、医疗和细胞免疫等领域受到广泛关注，在传感器阵列领域也有很大的应用潜力。近年来，传感器阵列因其在复杂生物分析和诊断中的应用而受到越来越多的关注。本综述首先概述了纳米酶的分类及其相应的类酶活性。然后介绍了纳米酶在各种传感器阵列中的应用，特别是作为非特异性识别受体的小分子、蛋白质和农药的检测。最后，展望了未来的发展方向和设计原则，以克服当前的挑战，从而为纳米酶传感器阵列的应用提供更多机会。

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引用次数: 0