Biosensors and Bioelectronics: X最新文献_第3页

Practical considerations for DNA sensing using Faradaic electrochemical impedance spectroscopy on ENIG PCB electrodes

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-12-17 DOI: 10.1016/j.biosx.2024.100569

Shruti Ahuja , Avani Kulkarni , Richa Pandey , Kiran Kondabagil , Siddharth Tallur

Electroless nickel immersion gold (ENIG) finish printed circuit board (PCB) substrates offer a cost-effective solution for electrochemical sensing of biomolecules. Electrochemical impedance spectroscopy (EIS) is highly sensitive and can differentiate between bioelectrochemical circuit elements over a wide frequency range. Traditional Faradaic EIS measurements often focus on charge transfer resistance

(R_{c t})

to determine analyte concentration. However, the long measurement time required to perform such EIS measurements down to very low frequencies (typically 0.1 Hz) can adversely affect the ENIG PCB due to gold layer degradation and copper corrosion. To overcome these issues, we propose using the constant phase element (CPE) in the electrical equivalent circuit in EIS measurements for DNA sensing. Our approach employs ENIG PCB electrodes functionalized with thiolated single-stranded DNA probes targeting the uidA gene of E. coli. We evaluate the specificity of this sensing scheme to a 166 bp complementary amplicon from E. coli against non-complementary amplicons of different lengths from E. coli and bacteriophage Phi6. By operating at higher frequencies (

>

10 Hz), CPE analysis reduces measurement times and minimizes the risk of PCB degradation. Preliminary findings indicate that the CPE impedance exhibits concentration-dependent changes with increasing target DNA concentrations. We also present preliminary results for utilizing this sensing mechanism to detect E. coli from wastewater samples.

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引用次数: 0

Electrochemical sensors for the detection of immune checkpoint related proteins and their role in cancer companion diagnostics

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-29 DOI: 10.1016/j.biosx.2024.100561

Louise Barnaby , Andrew G. Watts , Pedro Estrela

Cancer companion diagnostics are incredibly important in helping to determine whether a patient will benefit from immune checkpoint inhibitor (ICI) treatment. Determining the chances of treatment success helps to inform clinicians to make the best treatment decisions for a particular patient. Many immune checkpoint related proteins show potential as biomarkers for ICI success, such as the checkpoint proteins themselves, cytokines, interleukins and other immune response related proteins. The most investigated checkpoint inhibitor protein is Programmed Death Ligand 1 (PD-L1), which is used as a biomarker in clinical diagnostic tests but, with some limitations. In the near future, tests for many different biomarkers will start becoming commercially available along with tests for multiple biomarkers simultaneously, giving an even better prediction of potential ICI success. Electrochemical sensors are a high sensitivity point of care diagnostic technique that can have the potential to achieve detection of multiple biomarkers at once. The main problem facing this field is improving their sensitivity to be able to detect the incredibly low concentrations of biomarkers found in liquid biopsy samples. Many methods such as enhancing an electrode surface with high conductivity materials or increasing the measured electrochemical signal via signal amplifying molecules have been investigated with promising results. This review investigates the potential biomarkers relevant to predicting ICI success, as well as the current electrochemical sensors that have been developed to determine the expression levels of these proteins.

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引用次数: 0

Design of a polarization independent terahertz metamaterial absorber for biomedical sensing applications

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-29 DOI: 10.1016/j.biosx.2024.100560

Tasnim Mahfuz, Arif Hossan, Naymur Rahman

Metamaterial absorbers (MMAs) are currently highly desirable because of their special absorption qualities. In this paper, a highly simplified and optimized terahertz MMA is proposed and analyzed for sensing applications. The proposed MMA is polarization-independent because of its symmetrical structure. Its symmetrical structure is achieved through the utilization of L-shaped gold patches. Being polarization-independent means that terahertz waves of any polarization can be efficiently absorbed by it. The proposed design consists of several L-shaped gold metallic patches over a polyimide substrate and a ground plane. It achieves an absorption of 96% at a specific frequency of 1.672 THz with a quality factor of 28.26. The quality factor indicates a sharp resonance peak, which is crucial for accurate sensing measurements. The absorber's resonance frequency changes with its surrounding medium's refractive index (RI). Thus, the MMA can also be used as a RI sensor. The RI has varied in the range of 1.304–1.342. The average sensitivity of the proposed sensor is 374 GHz/RIU. It is important to note that most biomedical samples have RI ranges from 1.3 to 1.39, making the proposed sensor highly suitable for a broad range of biomedical applications. The biomedical applications include non-invasive detection of subtle changes within tissues, monitoring drug delivery processes or even early-stage disease diagnosis.

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引用次数: 0

In-SITE: In situ soil topological examination platform for hydration state, volumetric density and carbon stocks assessment In-SITE：用于水化状态、体积密度和碳储量评估的原位土壤地形学检查平台

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-14 DOI: 10.1016/j.biosx.2024.100559

Vikram Narayanan Dhamu , Diya Baby , Mohammed Eldeeb , Sriram Muthukumar , Shalini Prasad

This study presents a novel application of electrochemical impedance spectroscopy (EIS) for comprehensive soil health assessment, with a particular emphasis on bulk density (BD) analysis. Conventional methods for BD measurement, while effective, can be labor-intensive and prone to inaccuracies, especially in varying field conditions. In contrast, EIS offers a reliable, cost-effective, and non-invasive solution for real-time, in-situ measurements. The research outlines the use of Room Temperature Ionic Liquid (RTIL) functionalized sensors and the development of a portable hardware system for field deployment. Experimental protocols involved collecting impedance data by sweeping from 50 kHz to 5 Hz across various soil types to construct soil moisture profiles, and calibration curves demonstrated a strong correlation between impedance values and soil moisture content, validating the method's accuracy. The study introduces the DENSE model, which uses impedance data to predict soil volumetric density, with results closely matching standard mass/volume techniques. Further analysis reveals the complex relationships between soil moisture, BD, and impedance, producing calibrated models for accurate BD prediction. Additionally, the study extends to carbon stock analysis by integrating data on soil organic carbon percentage, BD, and soil depth to estimate carbon levels. Collectively, these findings underscore the potential of this EIS-based sensor as a versatile and precise tool for real-time soil health monitoring, offering significant contributions to precision agriculture and sustainable farming practices.

本研究介绍了电化学阻抗光谱（EIS）在土壤健康综合评估中的新应用，尤其侧重于容重（BD）分析。传统的体积密度测量方法虽然有效，但需要大量人力，而且容易出现误差，尤其是在不同的现场条件下。相比之下，EIS 为实时、原位测量提供了可靠、经济、无创的解决方案。该研究概述了室温离子液体（RTIL）功能化传感器的使用情况，以及用于现场部署的便携式硬件系统的开发情况。实验规程包括通过在各种土壤类型中以 50 kHz 至 5 Hz 的频率扫描来收集阻抗数据，以构建土壤湿度剖面，校准曲线显示阻抗值与土壤湿度之间存在很强的相关性，从而验证了该方法的准确性。研究介绍了 DENSE 模型，该模型使用阻抗数据预测土壤体积密度，结果与标准质量/体积技术非常接近。进一步的分析揭示了土壤湿度、BD 和阻抗之间的复杂关系，从而为准确预测 BD 建立了校准模型。此外，该研究还通过整合土壤有机碳百分比、BD 和土壤深度数据来估算碳含量，从而扩展到碳储量分析。总之，这些研究结果凸显了这种基于 EIS 的传感器作为实时土壤健康监测的多功能精确工具的潜力，为精准农业和可持续农业实践做出了重大贡献。

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引用次数: 0

Redox-active molecules in bacterial cultivation media produce photocurrent 细菌培养基中的氧化还原活性分子产生光电流

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-09 DOI: 10.1016/j.biosx.2024.100558

Matthew C. Smith , Nathan S. Nasseri , Emile J. Morin , Jakkarin Limwongyut , Alex S. Moreland , Yaniv Shlosberg , Andrea S. Carlini

Renewable energy concepts such as microbial fuel cells (MFCs) present a promising, yet intrinsically complex electrochemical approach for utilizing bacteria as an electron source. In this work, we show that just the cultivation media for bacterial growth, which is based on yeast extract, is sufficient for generating electrical current in a bio-electrochemical cell (BEC). We apply cyclic voltammetry and 2-dimensional fluorescence spectroscopy to identify redox active molecules such as NADH, NAD⁺, and flavines that may play key roles in electron donation. Finally, we show that upon illumination, current production is enhanced 2-fold. This photocurrent is generated by a variety of metabolites capable of photochemical reduction, enabling them to donate electrons at the anode of the BEC.

微生物燃料电池（MFC）等可再生能源概念为利用细菌作为电子源提供了一种前景广阔但内在复杂的电化学方法。在这项研究中，我们发现仅以酵母提取物为基础的细菌生长培养基就足以在生物电化学电池（BEC）中产生电流。我们应用循环伏安法和二维荧光光谱法确定了氧化还原活性分子，如 NADH、NAD+ 和黄素，它们可能在电子捐赠中发挥关键作用。最后，我们发现在光照下，电流产生增强了 2 倍。这种光电流是由多种能够进行光化学还原的代谢物产生的，从而使它们能够在 BEC 的阳极提供电子。

引用次数: 0

Assessing the accuracy of human-inspired electronic skin: A systematic review

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-05 DOI: 10.1016/j.biosx.2024.100553

Fahad AlShaibani , Vicente Grau , Jeroen Bergmann

Electronic skin (e-skin) systems are devices that mimic the different sensing modalities of skin. While the modalities sensed can vary from temperature to tactile, the main inspiration for much of the research being conducted is mimicking the sensing modalities of human skin. Much research has been conducted on tactile sensing through e-skin, as interest grows in the use of e-skin with smart prosthetics and Virtual and Augmented Reality (VR/AR) applications. Being able to mimic the sophisticated ability for human hands to complete complex tasks of dexterity, using an e-skin as essential input system, is the goal of many research groups. In this systematic review, we provide a full overview on e-skin systems that focus on developing tactile sensing. The objective is to assess how accurately these systems mimic human skin tactile sensing modalities and how accurately the detected stimuli are sensed. The outcomes of the review show where the focus of the community is with regards to which modalities are being developed. Furthermore, information extracted from the papers that detail their quantitative accuracy in sensing these modalities is provided. With a total of 205 systems included insights on trends and a quantitative comparison of current e-skin systems are discussed. The limitations of the current methods applied and how they could be overcome are explored, in addition to highlighting the need for standardized experimental protocols to ensure e-skin systems can be assessed and compared more easily.

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引用次数: 0

Printed dry and ready-to-use in vitro diagnostic culture media devices for differentiation and antimicrobial susceptibility testing of bacteria 用于细菌分化和抗菌药物敏感性测试的印刷干式即用型体外诊断培养基装置

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-11-01 DOI: 10.1016/j.biosx.2024.100557

Dieter Spiehl , Gerhard Schwall , Fabian Post , Carina Weber , Edgar Dörsam , Andreas Blaeser , Volkhard A.J. Kempf , Michael Hogardt

This work presents a ready-to use dry culture device platform for differentiation of bacterial species and testing antibiotic susceptibility conveniently. The study demonstrates that high-volume and scalable production methods such as can reliably manufacture such devices for reproducible in vitro diagnostic testing. This marks a significant advancement compared to similar handmade devices in the literature.

Two types of ready-to-use (RTU) devices were produced through printing. One device differentiates bacterial species from urine samples for rapid diagnosis of urinary tract infections (UTI), while the other determines the minimum inhibitory concentration (MIC) of antibiotics against specific bacterial species. The results show performance comparable to standard tests in clinical microbiological laboratories. The differentiation of bacterial species from four UTI samples matched the efficacy of commercial UTI agar plates utilizing chromogenic substrates. The MIC determination for seven bacterial species in three independent tests yielded categorical results consistent with MIC test strips on agar plates as reference standard. Minor errors occurred in 14 % and major errors in 5 % of the tests. No major errors occurred and in 81 % of all tests results were correct and consistent with the reference method.

这项研究提出了一种可随时使用的干培养装置平台，用于区分细菌种类和方便地测试抗生素敏感性。该研究表明，大批量、可扩展的生产方法，如......，可以可靠地制造出这种装置，用于可重复的体外诊断检测。与文献中的类似手工设备相比，这标志着一项重大进步。通过印刷生产出了两种即用型设备（RTU）。其中一种装置可从尿液样本中区分细菌种类，用于快速诊断尿路感染（UTI）；另一种装置可确定抗生素对特定细菌种类的最小抑菌浓度（MIC）。结果显示，其性能可与临床微生物实验室的标准测试相媲美。从四份 UTI 样品中区分细菌种类的结果与使用显色底物的商用 UTI 琼脂平板的效果相当。在三次独立测试中对 7 种细菌进行了 MIC 测定，得出的分类结果与作为参考标准的琼脂平板上的 MIC 测试条一致。有 14% 的测试出现了轻微误差，5% 的测试出现了重大误差。所有测试中没有出现重大错误，81%的测试结果正确且与参考方法一致。

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引用次数: 0

Development of novel DNA aptamers and colorimetric nanozyme aptasensor for targeting multi-drug-resistant, invasive Salmonella typhimurium strain SMC25 针对具有多重耐药性的侵袭性鼠伤寒沙门氏菌 SMC25 菌株开发新型 DNA 合剂和比色纳米酶合剂传感器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-24 DOI: 10.1016/j.biosx.2024.100555

Akanksha Joshi , Abhishek Kaushik , Neetu Kumra Taneja , Komal Chauhan , Amit Kumar , Tarun Kumar Sharma

Invasive, biofilm-forming Non-typhoidal Salmonella (iNTS), propagating through the global food and water supply chain, presents a significant risk to food safety and public health. Developing a robust detection system is crucial for enabling point-of-care, affordable, and equipment-free identification of this pathogen throughout the supply chain. In this study, we screened a novel pool of ssDNA aptamers specific to a multidrug resistant iNTS strain SMC25, previously isolated from Indian poultry products in our earlier research. Through 13 rounds of whole-cell SELEX, we identified, characterized, and selected seven full-length aptamers (ST18, ST19, ST25, ST28, ST29, ST31, and ST32). Flow cytometric analysis reveals superior binding of ST25, ST28, ST29, and ST31. These aptamers were translated onto Nanozyme-based aptasensing system for efficient, cost-effective detection of SMC25. This system harnesses the aptamer-mediated, reversible peroxidase-like activity of gold nanoparticles (GNPs) to oxidize the TMB substrate into a one-electron oxidation state, resulting in a blue-colored Diamine charge transfer complex (DCTC). The catalytic process, coupled with GNP aggregation, induces a visible color change in the test mixture from ruby-red to blue. Post-SELEX truncations identified the optimal aptamer sequence (T_ST31), which selectively detected SMC25 in water with a limit of detection (LOD) of ∼10⁴ CFU/mL. Lower concentrations (10 CFU/mL) of SMC25 could be detected after non-selective enrichment within 120 min. This research introduces a novel pool of iNTS-specific aptamers along with a cost-effective (0.25 USD per sample) solution for colorimetric detection by the naked eye.

通过全球食品和水供应链传播的侵袭性生物膜形成型非伤寒沙门氏菌（iNTS）对食品安全和公共卫生构成了重大风险。开发一种强大的检测系统对于在整个供应链中实现这种病原体的定点、经济和免设备鉴定至关重要。在本研究中，我们筛选了一个新型 ssDNA 类似物池，该类似物池特异于一种耐多药 iNTS 菌株 SMC25，在我们早期的研究中，该菌株曾从印度家禽产品中分离出来。通过 13 轮全细胞 SELEX，我们鉴定、表征并筛选出了七种全长适配体（ST18、ST19、ST25、ST28、ST29、ST31 和 ST32）。流式细胞分析显示，ST25、ST28、ST29 和 ST31 的结合力更强。这些适配体被转化为基于 Nanozyme 的适配体传感系统，用于高效、经济地检测 SMC25。该系统利用金纳米粒子（GNPs）的适配体介导的可逆过氧化物酶样活性，将 TMB 底物氧化成单电子氧化态，生成蓝色的二胺电荷转移复合物（DCTC）。这一催化过程加上 GNP 的聚集，使测试混合物的颜色从红宝石色变成了蓝色。经过 SELEX 截断，确定了最佳的适配体序列（T_ST31），它能选择性地检测水中的 SMC25，检测限（LOD）为 10⁴ CFU/mL。在 120 分钟内进行非选择性富集后，可检测到更低浓度（10 CFU/mL）的 SMC25。这项研究介绍了一种新型的 iNTS 特异性适配体库，以及一种具有成本效益（每个样品 0.25 美元）的肉眼比色检测解决方案。

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引用次数: 0

Performance of label-free biosensors as a function of layer thickness 无标记生物传感器的性能与层厚度的关系

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-19 DOI: 10.1016/j.biosx.2024.100556

Tommi Åman , Sanna Auer , Vesa P. Hytönen , Juha A. Määttä

Biosensors are used in various applications in the field of medicine, environmental monitoring, and chemical processing to study the concentration and quality of target molecules. Biosensors convert the interaction between a specific target molecule and a recognition element into a measurable signal. We compared the performance of four different label-free biosensor techniques (multi-parametric surface plasmon resonance (MP-SPR), quartz crystal microbalance (QCM), mass-sensitive micro array (MSMA, or also known as film bulk acoustic resonators (FBARs)) and biolayer interferometry (BLI)) to evaluate how well they can quantify thick protein layers. We utilized the avidin-biotin system, which enables tight and specific binding and allowed us to assemble layers of proteins on the biosensor surface in a well-defined and reproducible fashion. Our results show that MP-SPR outperforms the other label-free biosensors in analyzing thick samples, showing a predictable and sensitive binding signal for over 50 albumin – avidin layers, which is estimated to correspond to a 300–400 nm thick protein layer. The linear measurement range of BLI was 38 layers corresponding to a 228–304 nm thick surface on the biosensor while QCM and MSMA were able to measure 108–144 nm and 72–96 nm thick protein layers with a fairly linear response, respectively.

生物传感器广泛应用于医学、环境监测和化学处理领域，用于研究目标分子的浓度和质量。生物传感器将特定目标分子与识别元件之间的相互作用转化为可测量的信号。我们比较了四种不同的无标记生物传感器技术（多参数表面等离子体共振 (MP-SPR)、石英晶体微天平 (QCM)、质量敏感微阵列 (MSMA，又称薄膜体声谐振器 (FBAR))和生物层干涉测量法 (BLI)）的性能，以评估它们对厚蛋白质层的定量效果。我们使用了阿维丁-生物素系统，该系统可实现紧密而特异的结合，使我们能够以定义明确且可重复的方式在生物传感器表面组装蛋白质层。我们的研究结果表明，MP-SPR 在分析厚样品时优于其他无标记生物传感器，在超过 50 层白蛋白-鸟苷层（估计相当于 300-400 纳米厚的蛋白质层）上显示出可预测的灵敏结合信号。BLI 的线性测量范围为 38 层，相当于生物传感器上 228-304 纳米厚的表面，而 QCM 和 MSMA 能够分别测量 108-144 纳米和 72-96 纳米厚的蛋白质层，且反应相当线性。

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引用次数: 0

Simple and sensitive method for in vitro monitoring of red blood cell viscoelasticity by Quartz Crystal Microbalance with dissipation monitoring (QCM-D) 利用石英晶体微天平与耗散监测（QCM-D）对红细胞粘弹性进行体外监测的简单灵敏方法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2024-10-17 DOI: 10.1016/j.biosx.2024.100554

Simonetta Palleschi , Leopoldo Silvestroni , Barbara Rossi , Simone Dinarelli , Marco Magi , Lorenzo Giacomelli , Andrea Bettucci

Viscoelasticity (VE) is the intrinsic mechano-dynamic property enabling red blood cells (RBCs) to undergo prompt and repeated deformations while maintaining structural integrity. Assessing RBC VE and how different stressors can affect it is of great interest. Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) is a technology exploiting high-frequency acoustic waves to probe soft matter rheological properties. In the present study, a QCM-D method is reported for in vitro monitoring of cell VE in viable RBCs. The method is based on casting a sensor-adherent cell monolayer and modeling it as an effective viscoelastic medium, and allows to extrapolate proxy values of both the elastic and the viscous cell shear moduli. Real-time VE changes induced by the known cell VE stressors temperature, medium tonicity, glutaraldehyde, methyl-β-cyclodextrin and cytochalasin D have been reliably identified. The method is relatively simple and inexpensive, non-invasive, and able to seize subtle changes of cell biomechanics. Hence, it could be usefully exploited for in vitro assessment of RBC rheological properties and their alterations induced by external chemico-physical stimuli.

粘弹性（VE）是红细胞（RBC）在保持结构完整性的同时能够迅速、反复变形的内在机械动力特性。评估 RBC 的 VE 以及不同的应力如何影响它是非常有意义的。具有耗散监测功能的石英晶体微天平（QCM-D）是一种利用高频声波探测软物质流变特性的技术。本研究报告了一种用于体外监测存活 RBC 中细胞 VE 的 QCM-D 方法。该方法基于铸造一个传感器附着细胞单层，并将其建模为有效粘弹性介质，从而推断出细胞弹性和粘性剪切模量的代理值。已知的细胞粘弹性应激源温度、培养基补液度、戊二醛、甲基-β-环糊精和细胞松弛素 D 引起的实时粘弹性变化已被可靠地识别出来。该方法相对简单、廉价、无创，并能捕捉到细胞生物力学的微妙变化。因此，该方法可用于体外评估 RBC 流变特性及其在外部化学物理刺激下的变化。

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引用次数: 0