Biosensors and Bioelectronics: X最新文献_第9页

Portable cotton swab biosensor for rapid naked-eye detection of Helicobacter pylori 用于幽门螺杆菌快速肉眼检测的便携式棉签生物传感器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-24 DOI: 10.1016/j.biosx.2025.100636

Ghadeer.A.R.Y. Suaifan , Asmaa Alnajajrah , Ward Abu Jbara , Amr A. El-Mousa , Fahid Abu Jbara , Doha A.I. Al-Omari , Mayadah B. Shehadeh

To better understand the oral cavity's role as a potential reservoir and transmission route for Helicobacter pylori (H. pylori), the development of a diagnostic test that is not only rapid and highly specific but also user-friendly is essential. In response to this need, a colorimetric cotton swab biosensor was designed to enable visual detection of H. pylori presence or absence. This study presents the development of a rapid, simple and cost-effective cotton swab-based colorimetric biosensor for the visual detection of H. pylori in saliva. The biosensor utilizes a lactoferrin-functionalized cotton platform for bacterial capture, followed by signal generation using polymeric nanobeads conjugated to specific anti-H. pylori antibodies. The assay enables direct, naked-eye detection without the need for amplification steps or specialized instrumentation. A visual limit of detection between 10 CFU/mL was achieved within 5 min in solution and in spiked saliva samples, offering both qualitative and semi-quantitative results. The biosensor exhibited high specificity, showing no cross-reactivity with E. coli or S. aureus, and maintained analytical performance despite the presence of mucin in Saliva. Moreover, the device demonstrated operational stability over extended storage periods. These findings support the biosensor's utility as a point-of-care diagnostic tool in low-resource settings for the early detection and surveillance of H. pylori infections.

为了更好地了解口腔作为幽门螺杆菌（h.p ylori）的潜在宿主和传播途径的作用，开发一种不仅快速、高度特异性而且用户友好的诊断测试是必不可少的。针对这一需求，设计了一种比色棉签生物传感器，可以直观地检测幽门螺杆菌的存在或不存在。本研究提出了一种快速、简单、经济高效的棉签比色生物传感器，用于唾液中幽门螺杆菌的视觉检测。该生物传感器利用乳铁蛋白功能化的棉花平台进行细菌捕获，然后使用聚合纳米球偶联特异性抗h产生信号。螺杆菌抗体。该分析能够直接，裸眼检测，不需要扩增步骤或专门的仪器。在溶液和加标唾液样品中，在5分钟内达到10 CFU/mL的视觉检测限，提供定性和半定量结果。该生物传感器具有高特异性，与大肠杆菌或金黄色葡萄球菌无交叉反应性，即使唾液中存在粘蛋白也能保持分析性能。此外，该装置在延长的存储时间内表现出运行稳定性。这些发现支持了生物传感器在低资源环境中作为早期检测和监测幽门螺杆菌感染的即时诊断工具的实用性。

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引用次数: 0

A 3D-printed smartphone-based platform for in-situ and rapid monitoring of aquaculture pathogens using polydimethylsiloxane (PDMS) microchip with multiplex loop-mediated isothermal amplification (M-LAMP) 基于智能手机的3d打印水产养殖病原体原位和快速监测平台——多环介导等温扩增（M-LAMP）聚二甲基硅氧烷（PDMS）微芯片

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-15 DOI: 10.1016/j.biosx.2025.100635

Liyan Li , Jing Fu , Elaine Li Ching Chiang , Jerald Yoo , Sungwoo Bae

Aquaculture pathogens pose serious risks to aquatic livestock and global food safety. Key threats in shrimp farming include white spot syndrome virus (WSSV), Vibrio parahaemolyticus (causing acute hepatopancreatic necrosis disease, AHPND), and Enterocytozoon hepatopenaei (EHP). Rapid, on-site detection is critical for early detection and outbreak prevention. In this study, we developed a portable, smartphone-based diagnostic platform utilizing multiplex loop-mediated isothermal amplification (LAMP) for simultaneous detection of multiple pathogens in a single reaction. A PDMS microchip with 30 reaction wells (5 × 6 array) and a temperature control well was fabricated for efficient multiplexing. Immobilized LAMP reagents with freeze-drying lyophilization were preloaded into wells to streamline preparation and enhance stability. The system successfully identified both waterborne indicator bacteria (E. coli, E. faecalis, Salmonella) and major shrimp pathogens (WSSV, AHPND, EHP) in samples from Penaeus vannamei, Penaeus monodon, and aquaculture water. The microchip maintained stable isothermal conditions (65.1 ± 0.6 °C), enabling visual detection via color change at low DNA concentrations (as low as 4 copies/μL). All WSSV and EHP infections in shrimp tissues and water samples were correctly identified using LAMP reaction within 35 min (excluding the DNA extraction process), demonstrating 100% positive detection rates. The smartphone interface allowed real-time imaging and result interpretation, offering a rapid, user-friendly tool for in situ pathogen monitoring. This platform represents a practical, low-cost solution for field diagnostics and improved disease management in aquaculture.

水产养殖病原体对水生牲畜和全球食品安全构成严重威胁。对虾养殖的主要威胁包括白斑综合征病毒（WSSV）、副溶血性弧菌（引起急性肝胰腺坏死病，AHPND）和肝原肠胞虫（EHP）。快速的现场检测对于早期发现和预防疫情至关重要。在这项研究中，我们开发了一种便携式的、基于智能手机的诊断平台，利用多重环介导的等温扩增（LAMP）在单一反应中同时检测多种病原体。制作了具有30个反应孔（5 × 6阵列）和温度控制孔的PDMS微芯片，实现了高效复用。经冷冻干燥冻干的固定化LAMP试剂被预装入孔中，以简化制备流程并提高稳定性。该系统成功鉴定了凡纳滨对虾（Penaeus vannamei）、单斑对虾（Penaeus monodon）和养殖水体样品中的水生指示菌（E. coli、E. faecalis、Salmonella）和主要对虾病原体（WSSV、AHPND、EHP）。微芯片保持稳定的等温条件（65.1±0.6°C），可以在低DNA浓度（低至4拷贝/μL）下通过颜色变化进行视觉检测。在35 min内（不包括DNA提取过程），LAMP反应正确鉴定了虾组织和水样中所有WSSV和EHP感染，阳性率为100%。智能手机界面允许实时成像和结果解释，为原位病原体监测提供了快速，用户友好的工具。该平台为水产养殖现场诊断和改进疾病管理提供了一种实用、低成本的解决方案。

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引用次数: 0

Nanocrystal electrochemiluminescence Biosensor: Paving the way from lab discovery to market innovation 纳米晶体电化学发光生物传感器：从实验室发现到市场创新的道路

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-08 DOI: 10.1016/j.biosx.2025.100633

Abhishek Kumar , Sanket Goel

The commercial viability of a biosensor depends on its capability to accurately and precisely perform detection of target molecules in real physiological body fluids such as whole blood, serum, plasma, urine, saliva, tears, or sweat. A biosensor is considered ideal for detecting molecules if enriched with specific characteristics crucial for accurate outputs, namely low detection limit, high sensitivity, selectivity to target analyte, reproducibility and repeatability, and performance in real samples. Electrochemiluminescence (ECL) is a strong analytical technique with major applications in biosensing due to its inherent features from electrochemistry and photoluminescence. While existing ECL biosensors can deliver satisfactory performance in laboratory settings, only a limited number are effective in real complex matrices. The stability of biosensors in real samples is a significant concern, which often limits their commercial applications. Incorporation of nanomaterials in ECL biosensors has transformed the biomolecule detection process by providing unparalleled selectivity and sensitivity. This article deliberates on rendering contributions of nanomaterials in advancing traditional ECL biosensors to pave the way from lab discovery to market innovation. Furthermore, the article highlights the various roles of nanomaterials in addressing the critical challenges associated with the commercialization of ECL biosensors. Moreover, various essential concepts are highlighted with relevant figures and comparative tables to provide a general overview of the nanomaterial based ECL biosensors. Lastly, the future outlook and prospects of ECL biosensors in advancing molecular and clinical diagnostics is discussed.

生物传感器的商业可行性取决于其准确和精确地检测真实生理体液（如全血、血清、血浆、尿液、唾液、眼泪或汗液）中目标分子的能力。如果生物传感器具有对精确输出至关重要的特定特性，即低检测限、高灵敏度、对目标分析物的选择性、再现性和可重复性以及在实际样品中的性能，则被认为是检测分子的理想选择。电化学发光（ECL）是一种强大的分析技术，由于其固有的电化学和光致发光的特点，在生物传感领域有着重要的应用。虽然现有的ECL生物传感器可以在实验室环境中提供令人满意的性能，但只有有限数量的传感器在实际的复杂矩阵中有效。生物传感器在实际样品中的稳定性是一个重要的问题，这往往限制了它们的商业应用。纳米材料在ECL生物传感器中的结合，通过提供无与伦比的选择性和灵敏度，改变了生物分子检测过程。本文讨论了纳米材料在推进传统ECL生物传感器方面的贡献，为从实验室发现到市场创新铺平了道路。此外，文章强调了纳米材料在解决与ECL生物传感器商业化相关的关键挑战中的各种作用。此外，通过相关图表和比较表强调了各种基本概念，以提供基于纳米材料的ECL生物传感器的总体概述。最后，对ECL生物传感器在分子诊断和临床诊断中的应用前景进行了展望。

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引用次数: 0

Advancing food Safety: Two-plex electrochemical biosensor for mycotoxin detection in food matrices 推进食品安全：用于食品基质中霉菌毒素检测的双路电化学生物传感器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-06 DOI: 10.1016/j.biosx.2025.100626

Kundan Kumar Mishra , Vikram Narayanan Dhamu , Abhinav Kokala , Sriram Muthukumar , Shalini Prasad

Detecting foodborne toxins like Aflatoxin B1 and Zearalenone remains a pressing global health concern due to their impact on food safety. Conventional detection techniques lack the required sensitivity and efficiency, showcasing the urgent need for advanced, rapid detection solutions. This study presents a portable, non-faradaic electrochemical sensing platform specifically designed for quick and accurate detection of these toxins in corn flour. Featuring a short assay time of 5 min, the 2-plex platform leverages antibodies specific to Aflatoxin B1 and Zearalenone, achieving detection limits of 0.005 ng/mL and 0.05 ng/mL, respectively. The system offers a dynamic detection range of 0.01–9.151 ng/mL for Aflatoxin B1 and 0.1–25.6 ng/mL for Zearalenone. The platform demonstrates consistent performance, maintaining inter- and intra-study coefficient of variation (%CV) below 20 %. Validation against benchtop and outsource laboratory data confirms its real-world applicability. This user-friendly device holds promise for on-site toxin detection, enhancing food safety and public health.

由于黄曲霉毒素B1和玉米赤霉烯酮等食源性毒素对食品安全的影响，检测它们仍然是一个紧迫的全球卫生问题。传统的检测技术缺乏所需的灵敏度和效率，迫切需要先进、快速的检测解决方案。本研究提出了一种便携式非法拉第电化学传感平台，专门用于快速准确地检测玉米粉中的这些毒素。该平台利用黄曲霉毒素B1和玉米赤霉烯酮特异性抗体，检测时间短，仅需5分钟，检测限分别为0.005 ng/mL和0.05 ng/mL。该系统对黄曲霉毒素B1和玉米赤霉烯酮的动态检测范围分别为0.01 ~ 9.151 ng/mL和0.1 ~ 25.6 ng/mL。该平台表现出一致的性能，将研究间和研究内部的变异系数（%CV）保持在20%以下。对台式和外包实验室数据的验证确认了其在现实世界中的适用性。这种用户友好的设备有望进行现场毒素检测，加强食品安全和公众健康。

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引用次数: 0

Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection 镍掺杂壳聚糖纳米颗粒作为胰岛素电化学检测新平台的合成

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-03 DOI: 10.1016/j.biosx.2025.100624

Frederika Chovancová , Marjan Motiei , Ivana Šišoláková , Michal Urbánek , Jana Shepa , Haojie Fei , Petr Sáha , Renáta Oriňaková

Nickel modified chitosan nanoparticles are promising catalysts for the determination of bioanalytes such as insulin, glucose, antibiotics, and ascorbic acid. In this study, we synthesized nickel-loaded chitosan nanoparticles to evaluate their potential as surface modifiers for electrochemical sensors for insulin detection. The nanoparticles were prepared using the ionic gelation of chitosan with tripolyphosphate anions, followed by adsorption of nickel ions via ion-exchange resins and surface chelation. The physicochemical properties of the nanoparticles were characterized by scanning electron microscopy with EDX analysis, transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The catalytic activity of nickel modified chitosan nanoparticles towards insulin oxidation was investigated through cyclic voltammetry. The resulting screen-printed carbon electrode modified with nickel-chitosan nanoparticles exhibited exceptional analytical performance, including high sensitivity (0.09 mA μM), a low detection limit (0.02 μM), and a wide dynamic range (300 nM–5 μM). Additionally, the modified screen-printed electrode demonstrated excellent selectivity, enabling accurate insulin determination in the presence of interferences and in real blood serum samples. These findings highlight the potential of nickel-modified chitosan nanoparticles as a surface modification strategy to enhance the performance of electrochemical sensors insulin detection and pave the way for their application in various bioanalytes determination platforms.

镍修饰的壳聚糖纳米颗粒是测定胰岛素、葡萄糖、抗生素和抗坏血酸等生物分析物的有前途的催化剂。在这项研究中，我们合成了负载镍的壳聚糖纳米颗粒，以评估它们作为胰岛素检测电化学传感器表面改性剂的潜力。采用壳聚糖与三聚磷酸阴离子离子胶凝法制备纳米粒子，再通过离子交换树脂吸附镍离子并进行表面螯合。采用扫描电子显微镜、EDX分析、透射电子显微镜、傅里叶变换红外光谱和动态光散射等手段对纳米粒子的物理化学性质进行了表征。采用循环伏安法研究了镍修饰壳聚糖纳米颗粒对胰岛素氧化的催化活性。纳米镍壳聚糖修饰的丝网印刷碳电极具有高灵敏度（0.09 mA μM）、低检出限（0.02 μM）和宽动态范围（300 nM-5 μM）等优异的分析性能。此外，改进的丝网印刷电极具有优异的选择性，能够在存在干扰和真实血清样品的情况下准确测定胰岛素。这些发现突出了镍修饰壳聚糖纳米颗粒作为一种表面修饰策略的潜力，可以提高电化学传感器胰岛素检测的性能，并为其在各种生物分析物检测平台中的应用铺平了道路。

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引用次数: 0

SPARK - Sensor platform for affinity recognition of paraquat 用于百草枯亲和力识别的SPARK传感器平台

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-05-02 DOI: 10.1016/j.biosx.2025.100625

Durgasha C. Poudyal , Vikram Narayanan Dhamu , Manish Samson , Sumana Karmakar , Sriram Muthukumar , Shalini Prasad

The extensive use of paraquat (PQT) in agriculture has led to considerable environmental pollution and has raised serious health issues. Rapid, sensitive, and on-site detection tools are crucial for monitoring paraquat residue and preventing its toxic effect. This study focused on demonstrating a portable, label-free electrochemical sensor platform (SPARK) for the direct detection of paraquat using edamame soybean as food matrix. The sensor platform was fabricated using the excellent substrate property of a reduced graphene oxide (rGO), which was immobilized with the anti-paraquat antibody (PQT-Ab, specific to recognize the herbicide paraquat) using a linker molecule 1-pyrenebutanoic acid succinimidyl ester (Pyr-BASE). The rate of crosslinker adsorption on rGO was evaluated and demonstrated for the first time using electrochemistry, with the calculated monolayer adsorption of the pyrene-based crosslinker found to be 244 μC/cm². The sensor performance was tested using portable device platform (SPARK) to confirm its feasibility, which showed a limit of detection (LOD) of 0.3 ng/mL (0.3 ppb), dynamic range of PQT from 0.3 to 72.9 ng/mL and an r² value of 0.98. The cross-reactivity study demonstrates high selectivity for the target PQT antigen, in presence of non-specific antigen such as glyphosate and chlorpyrifos. Pearson's correlation of r = 0.99 indicated a strong positive correlation between SPARK platform and the third-party gold standard method. Developed SPARK platform provides sensitive data comparable to traditional analytical methods in a simplified manner, thereby opening the possibility for electrochemical sensor platform to be used as on-site testing devices for various other food matrices.

百草枯（PQT）在农业中的广泛使用导致了相当大的环境污染，并引发了严重的健康问题。快速、灵敏和现场检测工具是监测百草枯残留和预防其毒性作用的关键。本研究主要展示了一种便携式、无标签电化学传感器平台（SPARK），用于以毛豆为食品基质直接检测百草枯。该传感器平台是利用还原氧化石墨烯（rGO）的优良底物特性制成的，该平台与抗百草枯抗体（PQT-Ab，特异性识别除草剂百草枯）固定，并使用连接分子1-芘丁酸琥珀酰基酯（Pyr-BASE）。首次用电化学方法对交联剂在氧化石墨烯上的吸附速率进行了评价和验证，计算得到芘基交联剂的单层吸附量为244 μC/cm2。利用便携式仪器平台（SPARK）对传感器性能进行了测试，结果表明该传感器的检出限（LOD）为0.3 ng/mL (0.3 ppb)， PQT动态范围为0.3 ~ 72.9 ng/mL， r2值为0.98。交叉反应性研究表明，在草甘膦和毒死蜱等非特异性抗原存在的情况下，PQT靶抗原具有高选择性。皮尔逊相关r = 0.99，表明SPARK平台与第三方金标准方法呈正相关。开发的SPARK平台以简化的方式提供了与传统分析方法相当的敏感数据，从而为电化学传感器平台作为各种其他食品基质的现场测试设备打开了可能性。

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引用次数: 0

Nanotechnology-based paper microfluidics for rapid point-of-care detection and differentiation of snake venom types 基于纳米技术的纸微流体快速检测和区分蛇毒类型

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-21 DOI: 10.1016/j.biosx.2025.100623

Lakshmi Narashimhan Ramana , Nitin Salvi , M.V. Khadilkar , Tarun Kumar Sharma

Snake envenomation is recognized as a neglected tropical disease, contributing to high mortality rates and causing significant organ damage, particularly to the liver, kidneys, and brain. The primary treatment involves administering antivenom, which consists of polyclonal antibodies developed against various snake venoms. However, antivenom therapy can lead to serum-related complications, reducing its effectiveness. Therefore, targeting specific therapeutic molecules could significantly improve snake envenomation treatment. Identifying the snake species is a major challenge due to their similar morphological characteristics. Globally, only two snake venom diagnostic kits are available that have been developed to detect country-specific snake venom. Hence, there is an urgent need to develop new diagnostic assays tailored for detecting venom specific to India. To address this, the current study focuses on detecting functional enzyme components of venomous snake species, such as phospholipase A2, hyaluronidase, and proteases. The study is based on the loading of the dye-loaded stimuli-responsive nanoparticles, including liposomes (sensitive to phospholipase A2), hyaluronic acid-chitosan nanoparticles (sensitive to hyaluronidase), and casein nanoparticles (sensitive to proteases) into paper-based microfluidics and tested with various snake venoms. The device successfully detects and distinguishes between wet bites and dry bites, as well as viper and elapid species.

蛇中毒被认为是一种被忽视的热带病，造成高死亡率，并造成严重的器官损害，特别是对肝脏、肾脏和大脑。主要治疗包括使用抗蛇毒血清，它由针对各种蛇毒开发的多克隆抗体组成。然而，抗蛇毒血清治疗可导致血清相关并发症，降低其有效性。因此，针对特定的治疗分子可以显著改善蛇中毒的治疗。由于它们的形态特征相似，鉴定蛇的种类是一个主要的挑战。在全球范围内，只有两种用于检测特定国家蛇毒的蛇毒诊断试剂盒。因此，迫切需要开发新的诊断方法来检测印度特有的毒液。为了解决这个问题，目前的研究重点是检测毒蛇物种的功能酶成分，如磷脂酶A2，透明质酸酶和蛋白酶。该研究的基础是将染料负载的刺激响应纳米颗粒，包括脂质体（对磷脂酶A2敏感）、透明质酸-壳聚糖纳米颗粒（对透明质酸酶敏感）和酪蛋白纳米颗粒（对蛋白酶敏感）加载到基于纸的微流体中，并用各种蛇毒进行测试。该装置成功地检测和区分湿咬和干咬，以及毒蛇和蚜虫种类。

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引用次数: 0

Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker 3D打印微孔板混合微流控装置中的纸盘用于低成本、快速、超灵敏的纸基生物发光检测人表皮生长因子受体2 （HER2）乳腺癌生物标志物

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-16 DOI: 10.1016/j.biosx.2025.100621

Ahmed A. Shalaby , Asmaa Salah , Akihiko Ishida , Masatoshi Maeki , Manabu Tokeshi

Breast cancer is the most common cancer type in women and it has the highest probability of developing into invasive cancer. Early detection of breast cancer is crucial to reduce the disease burden and decrease the mortality rate. Detection of cancer biomarkers is an attractive non-invasive way to implement early diagnosis and follow-up. Colorimetric enzyme-linked immunosorbent assay (ELISA) is one of the most common techniques used for the detection of cancer biomarkers. However, it requires a long incubation time and a large reagent volume, and it has low sensitivity. Here, we propose use of a paper disc in a 3D printed microplate hybrid microfluidic device for ultrasensitive paper-based bioluminescence ELISA for detection of HER2 breast cancer biomarker. Chromatographic paper discs are good substrates for fast immobilization of capture antibody without making any surface modification and they can be replaced with new discs to reuse the 3D printed microplate. The 3D printed microplate has microvalves in the bottom of the wells, so it can stop flow of the reagents for the desired incubation time and it allows the washing solution to flow vertically and drain onto an adsorption pad which increases the washing efficiency. NanoLuc luciferase was used as a label for the detection antibody to achieve the highest sensitivity. Bioluminescence sandwich ELISA for HER2 detection was performed using the hybrid device in just 20 min and the limit of detection was 1.3 fg/mL which is more than 10⁴-fold better than commercial ELISA kits for HER2.

乳腺癌是女性中最常见的癌症类型，它发展为浸润性癌症的可能性最高。早期发现乳腺癌对于减轻疾病负担和降低死亡率至关重要。癌症生物标志物的检测是一种有吸引力的非侵入性方法，可以实现早期诊断和随访。比色酶联免疫吸附试验（ELISA）是检测癌症生物标志物最常用的技术之一。但需要孵育时间长，试剂量大，灵敏度低。在这里，我们提出在3D打印微孔板混合微流控装置中使用纸盘进行超灵敏的纸基生物发光ELISA检测HER2乳腺癌生物标志物。色谱纸圆盘是快速固定捕获抗体的良好底物，无需对表面进行任何修饰，并且可以更换新的圆盘以重复使用3D打印的微孔板。3D打印的微孔板底部有微阀，因此它可以在所需的孵卵时间内停止试剂的流动，并允许洗涤溶液垂直流动并排到吸附垫上，从而提高洗涤效率。采用NanoLuc荧光素酶作为检测抗体的标记物，灵敏度最高。混合装置可在20 min内完成HER2的生物发光夹心ELISA检测，检出限为1.3 fg/mL，比市售HER2 ELISA试剂盒提高104倍以上。

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引用次数: 0

Imatinib detection by memristive biosensors for therapeutic drug monitoring 记忆体生物传感器检测伊马替尼用于治疗药物监测

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-11 DOI: 10.1016/j.biosx.2025.100620

Junrui Chen , Lavinia Alberi , Yuan Pétermann , Thierry Buclin , Monia Guidi , Sandro Carrara

Therapeutic drug monitoring is essential for optimizing the efficacy and safety of targeted anticancer agents like imatinib, a first-line treatment for various leukemias and gastrointestinal stromal tumors. This study introduces a novel memristive biosensor designed for the detection of imatinib. The biosensor employs a silicon nanowire (SiNW) -based memristive architecture integrated with a single-stranded DNA (ssDNA) aptamer as the bio-recognition element. The detection of imatinib concentration is successfully demonstrated in both buffer and human plasma. Kinetic analysis reveals that the analysis time for achieving binding equilibrium and measurement is within 10 min. Comprehensive linear response over Imatinib concentrations in human plasma ranging from 0.2 μM to 20 μM was achieved, with a detection limit of 0.13 μM. While the interfering proteins such as human serum albumin (HSA) and α1-acid glycoprotein (AGP) compete with the binding mechanism, resulting in a decreased measured signal at lower concentrations of imatinib, their excessive presence paradoxically amplifies the measured signal. This amplification, however, also introduces increased variability in plasma measurements. This innovative memristive biosensor represents a significant advancement towards point-of-care therapeutic drug monitoring. It offers a robust and scalable platform, paving the way for the integration of personalized medicine into routine clinical workflows for imatinib-based therapies.

治疗药物监测对于优化伊马替尼等靶向抗癌药物的疗效和安全性至关重要，伊马替尼是各种白血病和胃肠道间质肿瘤的一线治疗药物。本研究介绍了一种用于检测伊马替尼的新型记忆体生物传感器。该生物传感器采用基于硅纳米线（SiNW）的忆阻结构，集成单链DNA （ssDNA）适体作为生物识别元件。在缓冲液和人血浆中成功地检测了伊马替尼的浓度。动力学分析表明，该方法可在10 min内实现结合平衡和测量，对人血浆中伊马替尼浓度在0.2 μM ~ 20 μM范围内实现全面线性响应，检出限为0.13 μM。虽然干扰蛋白如人血清白蛋白（HSA）和α1-酸性糖蛋白（AGP）与结合机制竞争，导致在低浓度伊马替尼下测量信号减弱，但它们的过量存在却矛盾地放大了测量信号。然而，这种放大也增加了等离子体测量的可变性。这种创新的记忆体生物传感器代表了护理点治疗药物监测的重大进步。它提供了一个强大且可扩展的平台，为将个性化医疗整合到伊马替尼治疗的常规临床工作流程中铺平了道路。

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引用次数: 0

Ionic Cell Microscopy: A new modality for visualizing cells using microfluidic impedance cytometry and generative artificial intelligence 离子细胞显微镜：一种利用微流控阻抗细胞术和生成式人工智能观察细胞的新方法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-05 DOI: 10.1016/j.biosx.2025.100619

Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard

This study introduces a novel approach to cancer cell imaging by integrating microfluidic sensor technology with artificial intelligence (AI). We developed a custom microfluidic device with polydimethylsiloxane (PDMS) microchannels and integrated electrodes to capture electrical impedance data. The device was fabricated using photolithography, electron beam evaporation, and lift-off techniques. Instead of traditional imaging methods, electrical impedance signals were used to reconstruct cell images. A generative AI model with eight hidden layers processed 191 impedance values to accurately reconstruct the shapes of cancer cells and control beads. Our approach successfully reconstructed images of MDA-MB-231 breast cancer cells, HeLa cells, and beads, achieving 91 % accuracy on the test dataset. Validation using the Structural Similarity Index (SSI) and Mean Structural Similarity Index (MSSIM) produced scores of 0.97 for breast cancer cells and 0.93 for beads, confirming the high precision of this method. This label-free, impedance-based imaging offers a promising solution for cancer diagnostics by accurately reconstructing cell shapes and distinguishing cell types, particularly in point-of-care applications.

本研究将微流体传感器技术与人工智能（AI）相结合，提出了一种新的癌细胞成像方法。我们开发了一种定制的微流控装置，该装置具有聚二甲基硅氧烷（PDMS）微通道和集成电极，用于捕获电阻抗数据。该装置采用光刻、电子束蒸发和发射技术制造。利用电阻抗信号代替传统的成像方法重建细胞图像。一个具有8个隐藏层的生成式AI模型处理了191个阻抗值，以准确地重建癌细胞和控制珠的形状。我们的方法成功地重建了MDA-MB-231乳腺癌细胞、HeLa细胞和微珠的图像，在测试数据集上达到了91%的准确率。使用结构相似指数（SSI）和平均结构相似指数（MSSIM）进行验证，乳腺癌细胞的得分为0.97，珠细胞的得分为0.93，证实了该方法的高精度。这种无标签、基于阻抗的成像技术通过精确重建细胞形状和区分细胞类型，为癌症诊断提供了一种很有前途的解决方案，特别是在即时护理应用中。

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引用次数: 0