Biosensors and Bioelectronics: X最新文献_第8页

Digital sensing technologies in cancer care: A new era in early detection and personalized diagnosis 数字传感技术在癌症治疗：早期检测和个性化诊断的新时代

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-25 DOI: 10.1016/j.biosx.2025.100651

Muge Yucel , Ahmet Önder , Tolunay Kurt , Berfin Keles , Merve Beyaz , Yaren Karadağ , İrem Yaşyerli , A. Irem Celik , Fatih Sema , Senem Tetik , Sanem Dinçkal , Soner Karabacak , Palaniappan Alagappan , Bo Liedberg , Umit Hakan Yildiz

Digital sensor platforms are systems that integrate sensors with digital technology, which revolutionize data collection, processing, and transmission for enabling real-time, high-precision and automated diagnostics. These platforms often serve as the backbone of modern monitoring systems, enabling real-time data acquisition and analysis for a wide range of applications. Recent advancements in digital sensor platforms have paved the way for transformative innovations in cancer diagnosis. These cutting-edge technologies offer unprecedented opportunities to facilitate early detection, improve diagnostic accuracy, and personalize treatment methods. This review explores the landscape of digital sensor platforms in the context of cancer diagnosis, providing an overview of their principles, functionalities, and clinical applications. The review further illustrates that biosensors, lab-on-a-chip (LOC) devices and wearable sensors have leveraged on nanotechnology, biorecognition materials and artificial intelligence (AI) for revolutionizing cancer diagnosis. It consolidates the recent advances in digital sensor platforms for cancer diagnosis and the associated critical challenges, such as regulatory concerns, standardization, and ethical considerations. Further, the review summarizes the feasibility for the integration of digital sensor platforms with routine clinical practices for the development of efficient cancer diagnosis and treatment methods.

数字传感器平台是将传感器与数字技术集成在一起的系统，它彻底改变了数据收集、处理和传输，实现了实时、高精度和自动化诊断。这些平台通常作为现代监控系统的支柱，为广泛的应用提供实时数据采集和分析。数字传感器平台的最新进展为癌症诊断的变革性创新铺平了道路。这些尖端技术为促进早期检测、提高诊断准确性和个性化治疗方法提供了前所未有的机会。这篇综述探讨了数字传感器平台在癌症诊断中的前景，概述了它们的原理、功能和临床应用。这篇综述进一步说明，生物传感器、芯片实验室（LOC）设备和可穿戴传感器已经利用纳米技术、生物识别材料和人工智能（AI）来彻底改变癌症诊断。它整合了用于癌症诊断的数字传感器平台的最新进展和相关的关键挑战，如监管问题、标准化和伦理考虑。此外，本文还总结了数字传感器平台与常规临床实践相结合的可行性，以开发高效的癌症诊断和治疗方法。

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引用次数: 0

Nanostructure modified carbon thread as wearable patch for enzymatic and non-invasive monitoring of glucose from human samples 纳米结构修饰的碳线可穿戴贴片用于人体样品中葡萄糖的酶促和无创监测

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-25 DOI: 10.1016/j.biosx.2025.100653

Jithin Thomas Chacko , K. Niyas , Anson T. Mathew , P Abdul Rasheed

Traditional blood-based glucose detection methods are invasive, inconvenient, and unsuitable for continuous monitoring, highlighting the critical need for a non-invasive alternative. To address this urgent need, we developed a non-invasive and wearable electrochemical glucose sensor patch based on a platinum-copper (Pt-Cu) nanocomposite electrodeposited on carbon cloth thread (CCT) electrode after functionalizing with an electropolymerized polyphenol (Pphe)-glucose oxidase (GOx) film. The immobilization of GOx via the formation of a polyphenol film was done by electropolymerization of phenol in presence of GOx. The detection capability of the developed sensor was evaluated by chronoamperometry analysis and found that the sensor is able to detect glucose with wide linear detection range from 100 to 1500 μM with an ultra-low limit of detection (LOD) of 31.1 μM and 64.4 μM for artificial sweat and urine respectively. In addition, the developed sensor is found to be reproducible, repeatable and highly selective in presence of common interfering molecules present in the human samples. Furthermore, the developed sensor was seamlessly integrated into a wearable prototype device on a commercial bandage with custom made potentiostat circuit called Nanopot. The developed wearable prototype sensor system also exhibits a wide linear detection range from 300 to 1500 μM and a promising LOD of 25.8 μM. These results further validate the reliability, consistency, and practical applicability of the sensor system with a promising potential to address the urgent demand for non-invasive, painless and user-friendly diabetes management.

传统的血糖检测方法具有侵入性、不方便且不适合连续监测，因此迫切需要一种非侵入性的替代方法。为了解决这一迫切需求，我们开发了一种无创可穿戴的电化学葡萄糖传感器贴片，该贴片基于铂-铜（Pt-Cu）纳米复合材料，经电聚合多酚(Pphe)-葡萄糖氧化酶（GOx）膜功能化后电沉积在碳布线（CCT）电极上。利用苯酚在氧化石墨烯存在下的电聚合，通过形成多酚膜来固定氧化石墨烯。通过计时安培分析对传感器的检测能力进行了评价，发现传感器能够在100 ~ 1500 μM的宽线性检测范围内检测葡萄糖，对人工汗液和尿液的超低检测限（LOD）分别为31.1 μM和64.4 μM。此外，开发的传感器被发现是可重复的，可重复的，在人类样品中存在的常见干扰分子的高度选择性。此外，开发的传感器被无缝集成到商业绷带上的可穿戴原型设备中，该设备带有定制的定位器电路Nanopot。所开发的可穿戴原型传感器系统还具有300 ~ 1500 μM的宽线性检测范围和25.8 μM的有前途的LOD。这些结果进一步验证了传感器系统的可靠性、一致性和实用性，在解决非侵入性、无痛性和用户友好型糖尿病管理的迫切需求方面具有很大的潜力。

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引用次数: 0

Design and analysis of a magneto-resistance-based device to mitigate risks from high magnetic field exposure 设计和分析一种基于磁电阻的装置，以减轻高磁场暴露的风险

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-25 DOI: 10.1016/j.biosx.2025.100654

Kazi Mustafizur Rahman, Md Mushfiqur Rahman, Sadia Islam, Hasib Md Abid Bin Farid, Md Faysal Nayan

The motivation is to develop a device for pacemaker-implanted patients that would automatically alert them in an intense magnetic field. Moreover, the employees working near any strong magnetic environment would benefit by avoiding high exposure. This research delves into a comprehensive process for the implementation and characterization of such a wearable based on the magnetoresistance effect, which is a function of the magnetic field. The program executes on the Arduino IDE platform. Samples are taken for varying magnetic flux density along each axis, for changes in distance of 2.5 mm. The calculations take place accordingly and provide outputs in microtesla units. Subsequently, the device is analyzed by plotting the responses, and it also helps to understand the working procedure. For a certain axis, the magnetic field is generally stronger than others. The goal is to determine the highest absolute value at any instance, including the Earth's geomagnetic field of 22–67 μT. Regulatory standards are followed to divide the magnetic flux density into four states: power saver (below 150 μT), safe (150–500 μT), unsafe (500–750 μT), and danger (over 750 μT). These values consist of ±20 μT error, which is quite insignificant. Depending on the state, the novel device generates different warning signals to mitigate risk from magnetic fields. From the error bar plot, it is realized that the percentage of error decreases while calculating higher magnetic flux. The errors could be reduced remarkably by ensuring better calibration and compensation techniques in the future.

其动机是为植入心脏起搏器的患者开发一种装置，该装置可以在强磁场中自动提醒他们。此外，在任何强磁环境附近工作的员工都可以避免高暴露。本研究基于磁场的函数磁阻效应，对这种可穿戴设备的实现和表征进行了全面的研究。该程序在Arduino IDE平台上执行。沿着每条轴的不同磁通密度采集样品，距离变化为2.5 mm。计算相应地进行，并提供以微特斯拉为单位的输出。随后，通过绘制响应来分析设备，也有助于了解工作流程。对于某个轴，磁场通常比其他轴强。目标是确定在任何情况下的最高绝对值，包括22-67 μT的地球地磁场。根据法规标准，将磁通密度分为节能（低于150 μT）、安全（150 ~ 500 μT）、不安全（500 ~ 750 μT）和危险（超过750 μT）四种状态。这些值由±20 μT的误差组成，这是相当不显著的。根据不同的状态，这种新型装置会产生不同的警告信号，以减轻磁场带来的风险。从误差柱状图中可以看出，计算较高磁通时，误差百分比减小。通过确保未来更好的校准和补偿技术，可以显著减少误差。

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引用次数: 0

MoS2-Si3N4-based SPR biosensor for the detection of malaria at different stages: A theoretical insight 基于mos2 - si3n4的SPR生物传感器在不同阶段检测疟疾：一个理论见解

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-25 DOI: 10.1016/j.biosx.2025.100655

Talia Tene , Marco Guevara , Isaías Caicedo , Jose Luis Granizo Jara , Myrian Borja , Lala Gahramanli , Cristian Vacacela Gomez , Stefano Bellucci

The accurate differentiation of malaria stages is essential for effective treatment and epidemiological control. This work presents a theoretical analysis of a multilayer surface plasmon resonance (SPR) biosensor for malaria stage detection based on refractive index variations of infected red blood cells. The sensor combines silver, silicon nitride (Si₃N₄), monolayer molybdenum disulfide (MoS₂), and thiol-functionalized single-stranded DNA (ssDNA) on a BK7 prism. Using the transfer matrix method (TMM), we evaluate the sensor performance across Ring, Trophozoite, and Schizont stages through sensitivity, full width at half maximum (FWHM), quality factor (QF), detection accuracy (DA), limit of detection (LoD), and comprehensive sensitivity factor (CSF). The optimized configuration achieves angular sensitivities of 318.2 (Ring), 268.9 (Trophozoite), and 244.8 (Schizont) °/RIU, demonstrating clear stage discrimination and competitive performance compared to reported multilayer SPR sensors. The layered architecture was designed with experimentally accessible materials and configurations, supporting future translation to stage-specific diagnostic platforms.

疟疾分期的准确区分对于有效治疗和流行病学控制至关重要。这项工作提出了一个多层表面等离子体共振（SPR）生物传感器疟疾阶段检测的理论分析，基于感染红细胞的折射率变化。该传感器在BK7棱镜上结合了银、氮化硅（Si3N4）、单层二硫化钼（MoS2）和硫醇功能化单链DNA （ssDNA）。利用传递矩阵法（TMM），我们通过灵敏度、半最大全宽度（FWHM）、质量因子（QF）、检测精度（DA）、检测限（LoD）和综合灵敏度因子（CSF）来评估环、Trophozoite和Schizont阶段的传感器性能。优化后的配置实现了318.2（环形）、268.9 （Trophozoite）和244.8 （Schizont）°/RIU的角灵敏度，与已有的多层SPR传感器相比，具有清晰的阶段识别和竞争力。分层架构采用实验可访问的材料和配置设计，支持未来转换为特定阶段的诊断平台。

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引用次数: 0

Smartphone-based colorimetric detection of urinary glucose using a novel green and red/green approach 基于智能手机的尿葡萄糖比色检测，采用一种新颖的绿色和红/绿方法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-24 DOI: 10.1016/j.biosx.2025.100650

Tileshwar Sahare, Simran Rana, Badri Narayana Sahoo, Milan Khanda, Abhijeet Joshi

Urine dipsticks are commonly used to detect pathological changes in urine due to their low cost and easy operation, having glucose parameter to detect glucosuria. Despite their advantages, the urine dipstick is ineffective in the early detection of diabetes due to its limitations in the detection of glucose below 100 mg/dl. Here, we present a novel, cost-effective ambient ring light-based smartphone platform to accurately estimate urinary glucose concentrations in lower points utilizing glucose sensors obtained from commercially available urine dipsticks (Mission® and Recombigen™). Glucose sensors changed color from green to brown with the increasing glucose concentration, resulting in a response time of 5 min. In this study, smartphone was used to capture images and analyze Red-Green-Blue (RGB) profiles, extracted with the help of an open-source smartphone-android-based Color Picker app. G intensity was determined to be inversely proportional and R/G intensity to be directly proportional to the increasing glucose concentration. LOD was found to be 6.2 and 24 mg/dl for Mission® glucose sensors with G and R/G respectively. On the other hand, Recombigen™ dipstick glucose sensors, LOD was evaluated as 5.8 and 16 mg/dl with G and R/G respectively. This smartphone-based assessment of glucose using dipsticks can be beneficial in the current screening and diagnostics sector of earlier detection of glucose in urine.

尿试纸成本低，操作方便，具有葡萄糖参数，可检测血糖，常用于检测尿液病理变化。尽管尿试纸有其优点，但由于其在检测葡萄糖低于100 mg/dl时的局限性，在糖尿病的早期检测中是无效的。在这里，我们提出了一种新颖的、具有成本效益的基于环境环光的智能手机平台，该平台利用从市售尿试纸（Mission®和Recombigen™）获得的葡萄糖传感器，准确估计低处的尿葡萄糖浓度。葡萄糖传感器的颜色随着葡萄糖浓度的增加从绿色变为棕色，导致响应时间为5分钟。在本研究中，使用智能手机捕获图像并分析红绿蓝（RGB）配置文件，并借助开源的基于智能手机android的color Picker应用程序提取。G强度被确定为反比，R/G强度与葡萄糖浓度的增加成正比。Mission®葡萄糖传感器的LOD分别为6.2和24 mg/dl，分别为G和R/G。另一方面，Recombigen™试纸式葡萄糖传感器的LOD分别以G和R/G计算为5.8和16 mg/dl。这种基于智能手机的葡萄糖评估使用试纸可以在当前的筛查和诊断领域早期检测尿液中的葡萄糖有益。

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引用次数: 0

β-Cyclodextrin-conjugated butein as a selective electrochemical molecular trafficking system for bilastine β-环糊精共轭丁蛋白作为胆碱的选择性电化学分子运输系统

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-18 DOI: 10.1016/j.biosx.2025.100647

Ramya Kanagaraj , Shanmugam SenthilKumar , Gururaja Perumal Pazhani , Murugan Veerapandian

Simple method for monitoring the therapeutic efficient concentration of anti-histamine drug, bilastine (BIL), is essential for rationalizing allergy and respiratory therapy. Herein, an external redox-mediator free molecular trafficking approach was developed using conjugated oligosaccharide-polyphenol system, β-cyclodextrin-butein (BCD-Bt). Mechanism behind the molecular loading of BIL within the hydrophobic cavity of BCD simultaneously enabling the redox-active signal transduction from the conjugated Bt are explored using cyclic and differential pulse voltammetric analyses. BCD-Bt sensor platform exemplified the selective loading of analyte BIL with a reliable detection linearity (nm to μM, R² = 0.981) and limit feasible for salivary therapeutic drug monitoring. Synergistic optical absorbance and emission-based molecular trafficking test further complements the multi-modal functionality of the demonstrated BCD-Bt in other similar molecular assays.

监测抗组胺药物bilastine （BIL）治疗有效浓度的简单方法，对变态反应和呼吸治疗的合理化至关重要。本研究利用偶联低聚糖-多酚体系β-环糊精-丁蛋白（BCD-Bt）建立了一种无氧化还原介质的外部分子运输方法。利用循环和差分脉冲伏安分析探讨了BCD疏水腔内BIL分子负载的机制，同时使共轭Bt的氧化还原活性信号转导成为可能。BCD-Bt传感器平台可选择性负载分析物BIL，具有可靠的检测线性（nm ~ μM, R2 = 0.981）和可行的唾液治疗药物监测限。协同光学吸收和基于发射的分子运输测试进一步补充了BCD-Bt在其他类似分子分析中的多模态功能。

引用次数: 0

A micro resonating motor based on neuron action potential 基于神经元动作电位的微共振马达

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-16 DOI: 10.1016/j.biosx.2025.100646

Antonio Carcaterra, Nicola Roveri, Silvia Milana, Gianluca Pepe

This paper introduces a novel bioelectromechanical device converting the electrochemical potential energy of excitable cells into mechanical work by coupling the Hodgkin–Huxley (HH) neuronal model response to a mechanical resonator. Addressing key challenges in bioelectromechanical systems, including biocompatibility, miniaturization, and efficient energy conversion, the device leverages the membrane potentials of biological cells to drive mechanical oscillations within microelectromechanical systems (MEMS). Through a combination of numerical simulations and theoretical analyses, it is demonstrated that the coupled HH–resonator system achieves stable limit cycles and significant mechanical displacements via parametric amplification. This amplification arises from the nonlinear capacitive coupling, which leads to Mathieu-like equations governing the system's dynamics, thereby enabling large oscillations from relatively small voltage inputs. Such parametric resonance is critical for the device's ability to sustain oscillatory motion, making it highly suitable for integration into compact and implantable MEMS applications. Potential applications include implantable sensors and actuators for real-time physiological monitoring, and advanced micro-scale systems that benefit from biologically sourced energy. The findings underscore the promise of bioelectromechanical systems in advancing biomedical and microengineering technologies, paving the way for innovative solutions in personalized medicine, bio-robotics, and beyond.

本文介绍了一种新型的生物机电装置，通过将霍奇金-赫胥黎（HH）神经元模型响应与机械谐振器耦合，将可兴奋细胞的电化学势能转化为机械功。该装置利用生物细胞的膜电位来驱动微机电系统（MEMS）内的机械振荡，解决了生物机电系统中的关键挑战，包括生物相容性、小型化和高效能量转换。通过数值模拟和理论分析相结合，证明了耦合hh谐振器系统通过参数放大获得稳定的极限环和显著的机械位移。这种放大是由非线性电容耦合引起的，这导致了控制系统动力学的类马蒂厄方程，从而使相对较小的电压输入产生大的振荡。这种参数共振对于器件维持振荡运动的能力至关重要，使其非常适合集成到紧凑和可植入的MEMS应用中。潜在的应用包括用于实时生理监测的植入式传感器和执行器，以及受益于生物能源的先进微尺度系统。这些发现强调了生物机电系统在推进生物医学和微工程技术方面的前景，为个性化医疗、生物机器人等领域的创新解决方案铺平了道路。

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引用次数: 0

Current state of the art on implantable sensors for continuous post-EVAR surveillance of abdominal aortic aneurysms: A comprehensive review 腹主动脉瘤evar后持续监测植入式传感器的研究现状：综述

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-12 DOI: 10.1016/j.biosx.2025.100645

G. Saroglia , S. Marocco , S. Nicoloso , S. Diana , M. Tozzi , I. Stefanini

Post-endovascular aneurysm repair (EVAR) surveillance currently relies on routine imaging techniques such as computed tomography angiography and magnetic resonance angiography, which expose patients to radiation, nephrotoxic contrast agents, and place logistical burdens on healthcare systems. This review critically examines the potential of implantable sensors, particularly pressure-monitoring systems, to provide continuous, minimally invasive post-EVAR surveillance. We present an overview of the biological and biomechanical rationale for monitoring aneurysm sac pressurization, analyze existing sensor technologies including EndoSure® and ImPressure®, and discuss technical challenges such as wireless telemetry, biocompatibility, signal attenuation, and power management. Regulatory milestones and alignment with ESVS and SVS surveillance guidelines are reviewed, along with the current evidence gap between pressure trends and clinical outcomes such as reintervention or rupture. While technical feasibility has been demonstrated in controlled settings, no studies to date have shown that pressure monitoring improves long-term outcomes or guides decision-making independently of imaging. The limitations of pressure-based monitoring are discussed alongside emerging sensor designs that integrate pressure, morphological, and biochemical sensing. This review concludes that implantable pressure sensors remain investigational tools whose clinical adoption will depend on future trials demonstrating diagnostic accuracy, patient acceptability, cost-effectiveness, and outcome benefit.

血管内动脉瘤修复后（EVAR）监测目前依赖于常规成像技术，如计算机断层血管造影和磁共振血管造影，这使患者暴露于辐射、肾毒性造影剂，并给医疗系统带来后勤负担。这篇综述严格审查了植入式传感器的潜力，特别是压力监测系统，以提供持续的、微创的evar后监测。我们概述了监测动脉瘤囊加压的生物学和生物力学原理，分析了现有的传感器技术，包括EndoSure®和ImPressure®，并讨论了无线遥测、生物相容性、信号衰减和电源管理等技术挑战。审查了监管里程碑和与ESVS和SVS监测指南的一致性，以及当前压力趋势与临床结果（如再干预或破裂）之间的证据差距。虽然技术上的可行性已经在受控环境下得到了证明，但迄今为止还没有研究表明压力监测可以改善长期结果或独立于成像指导决策。本文讨论了基于压力的监测的局限性以及集成压力、形态和生化传感的新兴传感器设计。本综述的结论是，植入式压力传感器仍然是研究工具，其临床采用将取决于未来的试验，以证明诊断的准确性、患者的可接受性、成本效益和结局效益。

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引用次数: 0

A versatile ITO electrode platform for studying neuronal differentiation and migration under electrical stimulation 研究电刺激下神经元分化和迁移的多功能ITO电极平台

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-09 DOI: 10.1016/j.biosx.2025.100637

Suthiwan Udomrat , Supeecha Kumkate , Permphan Dharmasaroja , Theeraporn Puntheeranurak , Tanakorn Osotchan

The electrical effects on cells, particularly neurons, are extensively studied to understand cellular functions. Various culture platforms have been developed to investigate cellular responses to electrical stimulation. In this study, planar indium tin oxide (ITO) interdigitated electrodes (IDEs) with conventional and circular designs were fabricated and utilized as both cell culture platforms and stimulation electrodes for experiments up to ten days. To enhance cell adhesion, fetal bovine serum (FBS) was applied to the electrode surface and then removed, facilitating cell culture. The SH-SY5Y cell line was cultured on the platform to assess adhesion and growth. Steady-state direct-current electric field stimulation (DCEFS) at 150 V/m or 300 V/m was applied at specific intervals. Cells exposed to 150 V/m showed significant morphological changes indicative of differentiation, while those at 300 V/m migrated away, reducing intact cell numbers. Notably, circular IDEs promoted extensive neurite outgrowth compared to conventional designs. These findings highlight the potential of electrode geometry to control cell differentiation and migration, offering a versatile platform for cellular studies and tissue engineering applications.

对细胞，特别是神经元的电效应进行了广泛的研究，以了解细胞功能。已经开发了各种培养平台来研究细胞对电刺激的反应。在这项研究中，平面氧化铟锡（ITO）交叉电极（IDEs）具有传统和圆形设计，并被用作细胞培养平台和刺激电极，实验时间长达10天。为了增强细胞粘附，将胎牛血清（FBS）涂于电极表面，然后去除，促进细胞培养。在平台上培养SH-SY5Y细胞系，观察其粘附和生长情况。以特定的间隔施加150 V/m或300 V/m的稳态直流电场刺激（DCEFS）。暴露于150 V/m的细胞表现出明显的形态变化，表明分化，而暴露于300 V/m的细胞则迁移，减少了完整细胞的数量。值得注意的是，与传统设计相比，圆形ide促进了广泛的神经突生长。这些发现突出了电极几何结构控制细胞分化和迁移的潜力，为细胞研究和组织工程应用提供了一个通用的平台。

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引用次数: 0

Rational design of peptides for epitope imprinting of polynorepinephrine: A plasmonic and machine learning integrated approach 多去甲肾上腺素表位印迹肽的合理设计：等离子体和机器学习的综合方法

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-06-06 DOI: 10.1016/j.biosx.2025.100638

Davide Sestaioni , Giulia Ciacci , Andrea Barucci , Pasquale Palladino , Simona Scarano

Molecularly Imprinted Polynorepinephrine (MIPNE) has demonstrated superior performance for mimetic receptors production, facilitating their integration into techniques like Surface Plasmon Resonance (SPR), Biomimetic Enzyme-Linked ImmunoSorbent Assay (BELISA), and Bio-Layer Interferometry (BLI). Here we developed a multiplexed Localized Surface Plasmon Resonance (LSPR) assay to face the selection of appropriate epitope sequences for protein imprinting, a critical factor in optimizing MIPNE efficiency. The plasmonic properties of gold nanoparticles formed on MIPNE were used to classify epitopes as functional (F), uncertain (U), or dysfunctional (D). Feature extraction and machine learning analysis identified key physico-chemical descriptors influencing imprinting efficiency. Subsequent SPR testing confirmed the correlation between epitope selection and receptor performance. This study provides the first systematic approach for epitope selection in MIPNE, paving the way for their improved design and application in bioanalytics and biosensing.

分子印迹多去甲肾上腺素（MIPNE）在模拟受体的生产中表现出优异的性能，促进了它们与表面等离子体共振（SPR）、仿生酶联免疫吸附测定（BELISA）和生物层干涉测定（BLI）等技术的整合。在这里，我们开发了一种多路局部表面等离子体共振（LSPR）方法来选择合适的表位序列进行蛋白质印迹，这是优化MIPNE效率的关键因素。在MIPNE上形成的金纳米颗粒的等离子体性质被用来将表位分类为功能性(F)、不确定性(U)或功能失调(D)。特征提取和机器学习分析确定了影响印迹效率的关键物理化学描述符。随后的SPR测试证实了表位选择与受体性能之间的相关性。本研究为MIPNE的表位选择提供了第一个系统的方法，为其在生物分析和生物传感中的改进设计和应用铺平了道路。

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引用次数: 0