Pub Date : 2020-11-11DOI: 10.21475/poj.13.02.20.2696
S. Jung, Y. Hur
Gibberellic acid (GA3) treatment is a useful method for inducing seedless grape berries in the seeded grape bunch before flowering. In this work, we applied 100 ppm of GA3 on ‘Tamnara’ grape flower cluster at 14 days before flowering to find metabolites significantly related to seedlessness. Three bunches of grape flower samples were collected at nine different stages (Day before full bloom; DBF13, 10, 7, 5, 2, flowering (0) and day after full bloom; DAF 2, 5, 9). Metabolites of each collected sample were analyzed using GC-MS with derivatization method (MSTFA). Metabolite contents of GA3 treatment flower were compared with non-treated controls in all stages and analyzed using Partial least squares discriminate analysis (PLS-DA). As a result, five sampling times (DBF 13, 10, 2, 0, DAF 9) showed significance differences using GA3 treatments. Total of 13 metabolites were recognized to relate to differences in five specific sampling times and mainly affected the initial stages (DBF 13 and 10). Tartaric acid, D-glucose, phosphoric acid, and D-mannose, inositol were increased by GA3 treatment at the early-flower developing stage. Dehydroascorbic acid, caffeic acid, citric acid, and gluconic acid were mainly increased at the time of GA3 treatment but decreased approaching full bloom. All stages of GA3 treatment, L-glutamine, L-serine, and D-allose was decreased, but fructose increased. In particular, the metabolite contents before GA3 treatment provides new clues on the role of GA3 in the early stage of grape berry development
{"title":"Metabolites changes after pre-bloom gibberellic acid (GA3) application for inducing seedless grape","authors":"S. Jung, Y. Hur","doi":"10.21475/poj.13.02.20.2696","DOIUrl":"https://doi.org/10.21475/poj.13.02.20.2696","url":null,"abstract":"Gibberellic acid (GA3) treatment is a useful method for inducing seedless grape berries in the seeded grape bunch before flowering. In this work, we applied 100 ppm of GA3 on ‘Tamnara’ grape flower cluster at 14 days before flowering to find metabolites significantly related to seedlessness. Three bunches of grape flower samples were collected at nine different stages (Day before full bloom; DBF13, 10, 7, 5, 2, flowering (0) and day after full bloom; DAF 2, 5, 9). Metabolites of each collected sample were analyzed using GC-MS with derivatization method (MSTFA). Metabolite contents of GA3 treatment flower were compared with non-treated controls in all stages and analyzed using Partial least squares discriminate analysis (PLS-DA). As a result, five sampling times (DBF 13, 10, 2, 0, DAF 9) showed significance differences using GA3 treatments. Total of 13 metabolites were recognized to relate to differences in five specific sampling times and mainly affected the initial stages (DBF 13 and 10). Tartaric acid, D-glucose, phosphoric acid, and D-mannose, inositol were increased by GA3 treatment at the early-flower developing stage. Dehydroascorbic acid, caffeic acid, citric acid, and gluconic acid were mainly increased at the time of GA3 treatment but decreased approaching full bloom. All stages of GA3 treatment, L-glutamine, L-serine, and D-allose was decreased, but fructose increased. In particular, the metabolite contents before GA3 treatment provides new clues on the role of GA3 in the early stage of grape berry development","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"436 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116706951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-11DOI: 10.21475/poj.13.02.20.3000
A. Al-Soqeer, S. Al-Otayk, M. Motawei
Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats. Cenchrus ciliaris is apomictic, a condition that is normally associated with low genetic variation within populations, but even moderate levels of genetic variation among populations could account for differences in invasiveness. We used Inter-Simple Sequence Repeat markers (ISSRs) to examine genetic variation among buffelgrass genotypes collected from nine different locations in the central region of Saudi Arabia, and three introduced cultivars. The genetic similarity coefficients among the twelve buffelgrass genotypes ranged from 0.66 to 0.94. Maximum similarity was observed between local populations. Introduced cultivars 'Biloela', 'Gayndah' and 'USA' were quite distinct from local populations. The dendogram constructed with UPGMA analysis revealed three clusters. In general, the study revealed the presence of considerable genetic diversity that could be used for further analysis for specific traits of interest.
{"title":"Molecular characterization of new Buffelgrass (Cenchrus ciliaris) genotypes","authors":"A. Al-Soqeer, S. Al-Otayk, M. Motawei","doi":"10.21475/poj.13.02.20.3000","DOIUrl":"https://doi.org/10.21475/poj.13.02.20.3000","url":null,"abstract":"Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats. Cenchrus ciliaris is apomictic, a condition that is normally associated with low genetic variation within populations, but even moderate levels of genetic variation among populations could account for differences in invasiveness. We used Inter-Simple Sequence Repeat markers (ISSRs) to examine genetic variation among buffelgrass genotypes collected from nine different locations in the central region of Saudi Arabia, and three introduced cultivars. The genetic similarity coefficients among the twelve buffelgrass genotypes ranged from 0.66 to 0.94. Maximum similarity was observed between local populations. Introduced cultivars 'Biloela', 'Gayndah' and 'USA' were quite distinct from local populations. The dendogram constructed with UPGMA analysis revealed three clusters. In general, the study revealed the presence of considerable genetic diversity that could be used for further analysis for specific traits of interest.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125037998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-11DOI: 10.21475/poj.13.02.20.2271
Ahmad Asnawi Mus, J. Gansau, V. Kumar, N. A. Rusdi
Phalaenopsis bellina, is an orchid widely known for their distinctive fragrance. Of late, fragrant orchids are getting more attention from Orchid breeders for their horticultural market value. Although volatile compounds have been identified in several orchid species, the floral scent emission of P. bellina is far from understood. Therefore, this study was carried out to investigate the influence of different timing and floral development stages on the volatile emissions from P. bellina flowers using Solid Phase Micro Extraction (SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). The volatile compound was extracted during morning and afternoon sessions from three different floral developmental stages. The volatile compounds emitted from flowers from two different sessions were trapped with SPME fiber for 30 minutes before directly injected into GC-MS for identification. Results showed that approximately 79 volatile compounds were identified, with the terpenoid presented as the major compound class. P. bellina had the highest number of volatiles during the morning and full bloom (41), with 29.82% monoterpene and 23.33% sesquiterpenes accounted in total. α-farnesene (19.56%) was abundance during morning emission and remains as the highest volatile in afternoon emission (44.08%), even higher when compared to morning emission. However, a decrease in the volatile compound was observed in afternoon emission, in which only 34 volatile compounds detected. Meanwhile, partial bloom developmental stage revealed linalool as the major terpenoid volatile compounds (25.89%), with only 20 volatile compounds recorded. On the other hand, no volatile compounds were recorded and profiled from flower bud stage. The establishment of a floral scent study provides a brief overview of the regulation of fragrance in P. bellina, which can be continued through gene isolation or fragrance-related enzymes study. This information will provide necessary information on orchid floral scent research that useful in boosting horticultural trade of the scented orchids and their function in pollination ecology study
{"title":"The variation of volatile compounds emitted from aromatic orchid (Phalaenopsis bellina) at different timing and flowering stages","authors":"Ahmad Asnawi Mus, J. Gansau, V. Kumar, N. A. Rusdi","doi":"10.21475/poj.13.02.20.2271","DOIUrl":"https://doi.org/10.21475/poj.13.02.20.2271","url":null,"abstract":"Phalaenopsis bellina, is an orchid widely known for their distinctive fragrance. Of late, fragrant orchids are getting more attention from Orchid breeders for their horticultural market value. Although volatile compounds have been identified in several orchid species, the floral scent emission of P. bellina is far from understood. Therefore, this study was carried out to investigate the influence of different timing and floral development stages on the volatile emissions from P. bellina flowers using Solid Phase Micro Extraction (SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). The volatile compound was extracted during morning and afternoon sessions from three different floral developmental stages. The volatile compounds emitted from flowers from two different sessions were trapped with SPME fiber for 30 minutes before directly injected into GC-MS for identification. Results showed that approximately 79 volatile compounds were identified, with the terpenoid presented as the major compound class. P. bellina had the highest number of volatiles during the morning and full bloom (41), with 29.82% monoterpene and 23.33% sesquiterpenes accounted in total. α-farnesene (19.56%) was abundance during morning emission and remains as the highest volatile in afternoon emission (44.08%), even higher when compared to morning emission. However, a decrease in the volatile compound was observed in afternoon emission, in which only 34 volatile compounds detected. Meanwhile, partial bloom developmental stage revealed linalool as the major terpenoid volatile compounds (25.89%), with only 20 volatile compounds recorded. On the other hand, no volatile compounds were recorded and profiled from flower bud stage. The establishment of a floral scent study provides a brief overview of the regulation of fragrance in P. bellina, which can be continued through gene isolation or fragrance-related enzymes study. This information will provide necessary information on orchid floral scent research that useful in boosting horticultural trade of the scented orchids and their function in pollination ecology study","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123576670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-11DOI: 10.21475/poj.13.02.20.2520
A. Elshafei, A. Alsadon, A. Al-Doss, Talal K. Al-Ateeq, T. Solieman, A. Ibrahim
This study evaluated the genetic diversity among 18 inbred lines of Pumpkin (Cucurbita moschata) using HFO-TAG (high frequency oligonucleotide–targeting active gene) markers. The inbred lines were selected from six different populations in Saudi Arabia and Egypt according to genetic distance and population origin. A total of 132 alleles were detected. Sixteen HFO-TAG markers averaged 8.25 alleles per primer with 125 alleles showing polymorphisms that averaged 7.81 alleles per primer. A cluster analysis of the means of the HFO-TAG data created three groups with similarity coefficients between 0.34 and 0.74. In general, the 18 inbred lines were separated according to pedigree and population origin. Hybrids from the chosen inbred lines, S1 L-1 with E L-2, S1 L-2 with E L-3, and S1 L-3 with E L-1, may be the basis for improved hybridization programs and can be used in future hybridization program
{"title":"Genetic diversity of Cucurbita moschata inbred lines selected from six different populations using HFO-TAG markers","authors":"A. Elshafei, A. Alsadon, A. Al-Doss, Talal K. Al-Ateeq, T. Solieman, A. Ibrahim","doi":"10.21475/poj.13.02.20.2520","DOIUrl":"https://doi.org/10.21475/poj.13.02.20.2520","url":null,"abstract":"This study evaluated the genetic diversity among 18 inbred lines of Pumpkin (Cucurbita moschata) using HFO-TAG (high frequency oligonucleotide–targeting active gene) markers. The inbred lines were selected from six different populations in Saudi Arabia and Egypt according to genetic distance and population origin. A total of 132 alleles were detected. Sixteen HFO-TAG markers averaged 8.25 alleles per primer with 125 alleles showing polymorphisms that averaged 7.81 alleles per primer. A cluster analysis of the means of the HFO-TAG data created three groups with similarity coefficients between 0.34 and 0.74. In general, the 18 inbred lines were separated according to pedigree and population origin. Hybrids from the chosen inbred lines, S1 L-1 with E L-2, S1 L-2 with E L-3, and S1 L-3 with E L-1, may be the basis for improved hybridization programs and can be used in future hybridization program","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124113584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P2517
R. Bertani, C. M. Joya, D. D. Henriquez, C. Funes, V. González, M. F. Perera, M. I. Cuenya, A. Castagnaro
The red stripe disease caused by Acidovorax avenae subsp. avenae in sugarcane, has become a quite relevant issue in Argentina because of its high incidence in the sugarcane growing area. The resistance of host plants is the most promising method for controlling the disease. In that sense, the Estación Experimental Agroindustrial Obispo Colombres (EEAOC) has a Sugarcane Breeding Program, which generates new varieties with higher productivity and good sanitary behavior. The lack of an effective screening technique to select resistant sugarcane genotypes limits the cultivar selection process. To develop a practical and affordable method for achieving the expression of the red stripe disease, three available inoculation techniques were evaluated under controlled conditions over two sugarcane varieties, with a previously adjustment of soil composition and nutrition and relative humidity. They consisted in (i) scrubbing the leaf surface with a cotton ball soaked in the suspension of A. avenae subsp. avenae; and spraying inoculum under two conditions: (ii) leaves pre-treated with a refined sand scarification and (iii) leaves with no scarification. Fifteen plants were inoculated per cultivar and treatment according to a randomized protocol with three replicates and the severity of the disease was evaluated on a scale of 1- 9 according to the International Society of Sugarcane Technologists. The spray inoculation using a bacterial suspension of A. avenae subsp. avenae without abrasives was also field tested. These results contribute to sugarcane breeding programs, providing a tool to assess the resistance to red stripe of their materials, overcoming the lack of bacterial pressure or favorable conditions for the disease.
{"title":"Assessment of inoculation techniques for screening sugarcane resistance to red stripe disease caused by Acidovorax avenae subsp. avenae","authors":"R. Bertani, C. M. Joya, D. D. Henriquez, C. Funes, V. González, M. F. Perera, M. I. Cuenya, A. Castagnaro","doi":"10.21475/AJCS.20.14.11.P2517","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P2517","url":null,"abstract":"The red stripe disease caused by Acidovorax avenae subsp. avenae in sugarcane, has become a quite relevant issue in Argentina because of its high incidence in the sugarcane growing area. The resistance of host plants is the most promising method for controlling the disease. In that sense, the Estación Experimental Agroindustrial Obispo Colombres (EEAOC) has a Sugarcane Breeding Program, which generates new varieties with higher productivity and good sanitary behavior. The lack of an effective screening technique to select resistant sugarcane genotypes limits the cultivar selection process. To develop a practical and affordable method for achieving the expression of the red stripe disease, three available inoculation techniques were evaluated under controlled conditions over two sugarcane varieties, with a previously adjustment of soil composition and nutrition and relative humidity. They consisted in (i) scrubbing the leaf surface with a cotton ball soaked in the suspension of A. avenae subsp. avenae; and spraying inoculum under two conditions: (ii) leaves pre-treated with a refined sand scarification and (iii) leaves with no scarification. Fifteen plants were inoculated per cultivar and treatment according to a randomized protocol with three replicates and the severity of the disease was evaluated on a scale of 1- 9 according to the International Society of Sugarcane Technologists. The spray inoculation using a bacterial suspension of A. avenae subsp. avenae without abrasives was also field tested. These results contribute to sugarcane breeding programs, providing a tool to assess the resistance to red stripe of their materials, overcoming the lack of bacterial pressure or favorable conditions for the disease.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117189586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P1961
S. Avivi, Bella Rhea Lavifa Sanjaya, S. Ogita, S. Hartatik, S. Soeparjono
In Indonesia, investigations of drought-tolerant cassava by characterizing the morphological, physiological, and molecular responses have not been carried out. This research was aimed to characterize the morphological, physiological, and molecular features of 10 Indonesian cassava varieties (Adira 1, Malang 1, Cimanggu, Kaspro, Ketan, Litbang UK-2, Malang 4, Malang 6, UJ-3, and UJ-5) under drought stress. 30 days after planting, drought stress was applied by stopping irrigation of plants for 15 days. The plant height, root system, and wilting were measured as morphological responses of cassava. For physiological responses, the percentage of opening stomata, chlorophyll, and hydrogen peroxide (H2O2) content were also investigated. Gene expression of MeGBF3 and MeMSD was evaluated to analyze the response of candidate drought-tolerant genotypes. The studied parameters revealed that drought stress inhibits the growth of cassava. Some genotypes could not survive because the excessive content of H2O2 would be toxic to plant cells and disturb the plant growth. The up-regulated gene expressions of MeGBF3 and MeMSD has correlations with morphological and physiological responses of cassava to drought stress. Adira 1 and Kaspro are thought as drought-tolerant genotypes due to the morphological, physiological, and molecular responses.
{"title":"Morphological, physiological and molecular responses of Indonesian cassava to drought stress","authors":"S. Avivi, Bella Rhea Lavifa Sanjaya, S. Ogita, S. Hartatik, S. Soeparjono","doi":"10.21475/AJCS.20.14.11.P1961","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P1961","url":null,"abstract":"In Indonesia, investigations of drought-tolerant cassava by characterizing the morphological, physiological, and molecular responses have not been carried out. This research was aimed to characterize the morphological, physiological, and molecular features of 10 Indonesian cassava varieties (Adira 1, Malang 1, Cimanggu, Kaspro, Ketan, Litbang UK-2, Malang 4, Malang 6, UJ-3, and UJ-5) under drought stress. 30 days after planting, drought stress was applied by stopping irrigation of plants for 15 days. The plant height, root system, and wilting were measured as morphological responses of cassava. For physiological responses, the percentage of opening stomata, chlorophyll, and hydrogen peroxide (H2O2) content were also investigated. Gene expression of MeGBF3 and MeMSD was evaluated to analyze the response of candidate drought-tolerant genotypes. The studied parameters revealed that drought stress inhibits the growth of cassava. Some genotypes could not survive because the excessive content of H2O2 would be toxic to plant cells and disturb the plant growth. The up-regulated gene expressions of MeGBF3 and MeMSD has correlations with morphological and physiological responses of cassava to drought stress. Adira 1 and Kaspro are thought as drought-tolerant genotypes due to the morphological, physiological, and molecular responses.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127954578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P2469
W. Gomes, S. Pimenta, P. S. C. Mascarenhas, L. N. Londe, Arles Matheus Pickler de Barros do Vale, S. A. Souza, H. Monteiro, B. Rodrigues, L. Vespoli, Osmer Balam
The propagation of plants through stem subdivision and the incorporation of phytoregulators can be a viable and efficient technique in the large-scale production of young forage palm at a lower cost. This study was conducted in order to evaluate the influence of stem subdivision and phytoregulators on the production of young forage palms under greenhouse conditions. Three experiments (I, II and III) were performed. Experiments I and II consisted of young palm of the Miúda genotype, formed from fragmented stems submitted to solutions containing gradually increasing concentrations of benzylaminopurine (0,0; 0,5; 1,0 e 1,5 mg. L-1) and kinetin (0,0; 0,25; 0,5 e 0,75 mg. L-1) combined with naphthaleneacetic acid (1,5 mg. L-1). An additional control without the use of phytoregulators was also included. These treatments were distributed in a randomized block design (RBD), in a factorial scheme with the additional plot: 4 x 4 + 1. In experiment III, different sized fractions of four forage palm genotypes were tested: Gigante, Orelha de elefante, IPA Sertânia, and Miúda. For this test, the experimental design was RBD, with twelve treatments, distributed in five blocks with five plants per plot. There was no influence of phytoregulators on the characteristics evaluated in experiments I and II. Significant differences were found. However, for the variables length, width, and thickness of sprouts among treatments in experiment III. Therefore, the application of phytoregulators in young forage palms at the dosages used is not recommended. For the Gigante, Orelha de Elefante and Miúda genotypes, the fractional stem size recommended is 5x3 cm whereas for IPA Sertânia, the size recommended is 4x2 cm.
通过茎的细分和植物调节剂的掺入繁殖是一种可行而有效的技术，可以在较低的成本下大规模生产牧草棕榈幼树。本研究旨在探讨温室条件下茎秆细分和植物调节剂对饲用棕榈幼树生产的影响。进行了3个实验(1、2、3)。实验一和实验二由Miúda基因型的幼树组成，幼树是由破碎的茎段置于浓度逐渐增加的苄氨基嘌呤溶液中形成的(0,0;0、5;1,0或1,5毫克。L-1)和激动素(0,0;0, 25;0,5或0,75毫克。L-1)与萘乙酸(1.5 mg)结合。l - 1)。还包括不使用植物调节剂的额外对照。这些处理在随机区组设计(RBD)中分布，在一个附加图:4 x 4 + 1的析因方案中。实验三对四种饲用棕榈基因型(Gigante、Orelha de elefante、IPA sertnia和Miúda)的不同大小组分进行了检测。本试验采用RBD试验设计，12个处理，分布在5个小区，每个小区5株。植物调节剂对试验ⅰ和试验ⅱ评价的性状没有影响。发现了显著差异。然而，试验三处理间芽的长度、宽度和厚度变量。因此，不建议在草木幼树中施用植物调节剂。对于Gigante, Orelha de Elefante和Miúda基因型，推荐的分茎大小为5x3 cm，而对于IPA sert nia，推荐的大小为4x2 cm。
{"title":"Phytoregulators and stem subdivision in the production of young forage palms (Opuntia ficus-indica and Nopalea cochellinifera)","authors":"W. Gomes, S. Pimenta, P. S. C. Mascarenhas, L. N. Londe, Arles Matheus Pickler de Barros do Vale, S. A. Souza, H. Monteiro, B. Rodrigues, L. Vespoli, Osmer Balam","doi":"10.21475/AJCS.20.14.11.P2469","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P2469","url":null,"abstract":"The propagation of plants through stem subdivision and the incorporation of phytoregulators can be a viable and efficient technique in the large-scale production of young forage palm at a lower cost. This study was conducted in order to evaluate the influence of stem subdivision and phytoregulators on the production of young forage palms under greenhouse conditions. Three experiments (I, II and III) were performed. Experiments I and II consisted of young palm of the Miúda genotype, formed from fragmented stems submitted to solutions containing gradually increasing concentrations of benzylaminopurine (0,0; 0,5; 1,0 e 1,5 mg. L-1) and kinetin (0,0; 0,25; 0,5 e 0,75 mg. L-1) combined with naphthaleneacetic acid (1,5 mg. L-1). An additional control without the use of phytoregulators was also included. These treatments were distributed in a randomized block design (RBD), in a factorial scheme with the additional plot: 4 x 4 + 1. In experiment III, different sized fractions of four forage palm genotypes were tested: Gigante, Orelha de elefante, IPA Sertânia, and Miúda. For this test, the experimental design was RBD, with twelve treatments, distributed in five blocks with five plants per plot. There was no influence of phytoregulators on the characteristics evaluated in experiments I and II. Significant differences were found. However, for the variables length, width, and thickness of sprouts among treatments in experiment III. Therefore, the application of phytoregulators in young forage palms at the dosages used is not recommended. For the Gigante, Orelha de Elefante and Miúda genotypes, the fractional stem size recommended is 5x3 cm whereas for IPA Sertânia, the size recommended is 4x2 cm.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129983836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P2323
André Cayô Cavalcanti, F. Partelli, I. Gontijo, Julien Chiquieri, H. Braun
The Diagnosis and Recommendation Integrated System (DRIS) is a tool to assess the nutritional state of plants. Due to the decrease of soil fertility in pasture areas and little information about fertilization recommendations, the nutritional reference norms for soil and sufficiency range can be useful tools to help future fertilization. Norms DRIS has been proved efficient as a method for nutritional diagnosis in several crops. However, there are a lack of information on the use of DRIS and sufficiency range for Latosols and Acrisols cultivated with Urochloa brizantha. Thus, the objective of the present study was to establish reference nutritional norms using sufficiency ranges and DRIS norms for Latosols and Acrisols cultivated with Urochloa brizantha and their use in nutritional diagnosis. Soils samples from 20 Urochloa brizantha pastures sites of the North of Espírito Santo State were used to establish the reference norms, and a further 85 soils samples were randomly collected for diagnosis analysis, in order to characterize nutritional condition of pastures. DRIS norms and sufficiency ranges were established for Latosols and Acrisols cultivated with U. brizantha pastures. The differences found between soil norms for other Brazilian regions allow us to infer that the norms should be used only in the conditions in which they were developed. Our results suggest that using DRIS norms and sufficiency ranges developed on the basis of soil analysis revealed deficiency of P, B, Cu, and Zn in more than 40% of the pastures cultivated with U. brizantha and 47% of the areas needed liming.
{"title":"Reference standards for soils cultivated with Urochloa brizantha and its use in nutritional diagnosis","authors":"André Cayô Cavalcanti, F. Partelli, I. Gontijo, Julien Chiquieri, H. Braun","doi":"10.21475/AJCS.20.14.11.P2323","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P2323","url":null,"abstract":"The Diagnosis and Recommendation Integrated System (DRIS) is a tool to assess the nutritional state of plants. Due to the decrease of soil fertility in pasture areas and little information about fertilization recommendations, the nutritional reference norms for soil and sufficiency range can be useful tools to help future fertilization. Norms DRIS has been proved efficient as a method for nutritional diagnosis in several crops. However, there are a lack of information on the use of DRIS and sufficiency range for Latosols and Acrisols cultivated with Urochloa brizantha. Thus, the objective of the present study was to establish reference nutritional norms using sufficiency ranges and DRIS norms for Latosols and Acrisols cultivated with Urochloa brizantha and their use in nutritional diagnosis. Soils samples from 20 Urochloa brizantha pastures sites of the North of Espírito Santo State were used to establish the reference norms, and a further 85 soils samples were randomly collected for diagnosis analysis, in order to characterize nutritional condition of pastures. DRIS norms and sufficiency ranges were established for Latosols and Acrisols cultivated with U. brizantha pastures. The differences found between soil norms for other Brazilian regions allow us to infer that the norms should be used only in the conditions in which they were developed. Our results suggest that using DRIS norms and sufficiency ranges developed on the basis of soil analysis revealed deficiency of P, B, Cu, and Zn in more than 40% of the pastures cultivated with U. brizantha and 47% of the areas needed liming.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133609382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P2535
Nayara Christina Almeida Araújo, L. A. Frazão, I. C. Freitas, E. Ferreira, Daniela Aparecida Freitas, M. V. Santos, D. Sanglard, L. A. Fernandes
The objective of this study was to evaluate the chemical attributes and quality of an Oxisol after one year of conversion of degraded pasture into integrated production system. The evaluated treatments were degraded pasture (PAST-Control); Eucalyptus, clone Urograndis 144 (Eucalyptus grandis x E. urophylla hybrid) intercropped with cor and marandu grass (Brachiaria brizantha) (integration crop-livestock-forest system - ICLFS-M); with maize and perennial horse gram (Macrotyloma axillare) (ICLFS-HG); and with maize, java and marandu grass (ICLFS-M+J); Monoculture of marandu grass (MAR) and perennial horse gram (HG); and marandu grass intercropped with Java/ perennial horse gram (H+M). Soil samples were collected in July/2015 and January/2016 in 0-5, 5-10, 10-20 and 20-30 cm soil depth layers. The soil attributes such as pH, organic matter, phosphorus, sum of bases, effective and potential cation exchange capacity and base saturation were evaluated. The implantation of ICLFS system contributed to increase of soil organic matter, sum of bases, effective and potential cation exchange capacity and soil base saturation. The soil biological activity was increased in the rainy season, and the soil microbial carbon increased in ICLFS-HG+M, ICLFS- HG, ICLFS-M and HG+M when compared to monocultures and PAST. Integrated production systems provide improved in soil quality even with a short time implementation.
本研究的目的是评价退化草场转化为综合生产系统一年后的Oxisol的化学特性和质量。评价处理为退化草场(PAST-Control);桉树无性系Urograndis 144 (grandis x E. urophylla杂种)间作cor和marandu草(Brachiaria brizantha)(作物-牲畜-森林一体化系统- ICLFS-M)与玉米和多年生马草(Macrotyloma axilla) (ICLFS-HG);玉米、爪哇和马兰度草(ICLFS-M+J);马兰度草(MAR)与多年生马蹄草(HG)的单一栽培间作爪哇/多年生马草(H+M)。土壤样品采集时间为2015年7月和2016年1月，土壤深度为0-5、5-10、10-20和20-30 cm。评价了土壤pH、有机质、磷、碱基总量、有效和潜在阳离子交换容量、碱基饱和度等土壤属性。ICLFS系统的植入增加了土壤有机质、碱基总量、有效阳离子交换量和潜在阳离子交换量以及土壤碱基饱和度。雨季土壤生物活性增加，ICLFS-HG+M、ICLFS-HG、ICLFS-M和HG+M土壤微生物碳含量较单作和过去增加。综合生产系统即使在短时间内实施也能改善土壤质量。
{"title":"Soil chemical and microbiological attributes under integrated production system in Oxisol of degraded pasture","authors":"Nayara Christina Almeida Araújo, L. A. Frazão, I. C. Freitas, E. Ferreira, Daniela Aparecida Freitas, M. V. Santos, D. Sanglard, L. A. Fernandes","doi":"10.21475/AJCS.20.14.11.P2535","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P2535","url":null,"abstract":"The objective of this study was to evaluate the chemical attributes and quality of an Oxisol after one year of conversion of degraded pasture into integrated production system. The evaluated treatments were degraded pasture (PAST-Control); Eucalyptus, clone Urograndis 144 (Eucalyptus grandis x E. urophylla hybrid) intercropped with cor and marandu grass (Brachiaria brizantha) (integration crop-livestock-forest system - ICLFS-M); with maize and perennial horse gram (Macrotyloma axillare) (ICLFS-HG); and with maize, java and marandu grass (ICLFS-M+J); Monoculture of marandu grass (MAR) and perennial horse gram (HG); and marandu grass intercropped with Java/ perennial horse gram (H+M). Soil samples were collected in July/2015 and January/2016 in 0-5, 5-10, 10-20 and 20-30 cm soil depth layers. The soil attributes such as pH, organic matter, phosphorus, sum of bases, effective and potential cation exchange capacity and base saturation were evaluated. The implantation of ICLFS system contributed to increase of soil organic matter, sum of bases, effective and potential cation exchange capacity and soil base saturation. The soil biological activity was increased in the rainy season, and the soil microbial carbon increased in ICLFS-HG+M, ICLFS- HG, ICLFS-M and HG+M when compared to monocultures and PAST. Integrated production systems provide improved in soil quality even with a short time implementation.","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131416246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-10DOI: 10.21475/AJCS.20.14.11.P2891
Luana Paula Freire Souza, M. T. D. Paula, C. Veloso, E. J. M. Carvalho, A. R. Silva, A. N. Pontes, Marcelo Jose Raiol Souza, C. Urbinati, Henrique Nunes, P. Simões
The objective of this work was to evaluate the efficiency of nitrogen fertilization in relation to the population of corn plants in a yellow latosol under no-tillage system. The experimental design was a randomized block with three replications, in a 4 x 4 factorial scheme, corresponding to four levels of N (0, 60, kg haˉ¹ 120 and 180) and four planting densities (45,000; 55,000; 65,000 and 75,000 plants haˉ¹). 48 soil samples were collected to determine chemical soil attributes (pH, OM, K, Ca, Na, Mg and Al) and 48 leaf samples to determine macronutrients in the leaf (N, P, K, Na, Ca and Mg). The data were submitted to comparison of means by the Scott-Knott test. The corn yield increased linearly up to the dose of 180 kg haˉ¹ N. Higher productivity was observed with the combination of 120 and 180 N (kg haˉ¹) with the density of 75,000 plants ha-1. There was an increase in the N content in the leaves with increasing doses of N in the soil
本研究的目的是评价免耕条件下黄壤中氮肥施用效率与玉米种群的关系。试验设计为随机区组，3个重复，4 × 4因子方案，对应4个N水平(0、60、kg ha - 1¹120和180)和4个种植密度(45000;55000;6万5千到7万5千株植物。采集土壤样品48份，测定土壤化学属性(pH、OM、K、Ca、Na、Mg和Al);采集叶片样品48份，测定叶片宏量营养元素(N、P、K、Na、Ca和Mg)。数据通过Scott-Knott检验进行均值比较。随着施氮量的增加，玉米产量呈线性增加趋势，在施氮量为180 kg ha-1时，施氮量为120和180 (kg ha-1)，施氮密度为7.5万株ha-1时，产量较高。随着土壤施氮量的增加，叶片中氮含量增加
{"title":"Evaluation of nitrogen fertilization for planting corn in a yellow latosol under no-tillage system","authors":"Luana Paula Freire Souza, M. T. D. Paula, C. Veloso, E. J. M. Carvalho, A. R. Silva, A. N. Pontes, Marcelo Jose Raiol Souza, C. Urbinati, Henrique Nunes, P. Simões","doi":"10.21475/AJCS.20.14.11.P2891","DOIUrl":"https://doi.org/10.21475/AJCS.20.14.11.P2891","url":null,"abstract":"The objective of this work was to evaluate the efficiency of nitrogen fertilization in relation to the population of corn plants in a yellow latosol under no-tillage system. The experimental design was a randomized block with three replications, in a 4 x 4 factorial scheme, corresponding to four levels of N (0, 60, kg haˉ¹ 120 and 180) and four planting densities (45,000; 55,000; 65,000 and 75,000 plants haˉ¹). 48 soil samples were collected to determine chemical soil attributes (pH, OM, K, Ca, Na, Mg and Al) and 48 leaf samples to determine macronutrients in the leaf (N, P, K, Na, Ca and Mg). The data were submitted to comparison of means by the Scott-Knott test. The corn yield increased linearly up to the dose of 180 kg haˉ¹ N. Higher productivity was observed with the combination of 120 and 180 N (kg haˉ¹) with the density of 75,000 plants ha-1. There was an increase in the N content in the leaves with increasing doses of N in the soil","PeriodicalId":277149,"journal":{"name":"November 2020","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115638281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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