Colloids and Surfaces B: Biointerfaces最新文献_第5页

Emulsifying behavior of plant proteins investigated via surface hydrophobicity and molecular weight distribution 通过表面疏水性和分子量分布研究植物蛋白的乳化行为。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-29 DOI: 10.1016/j.colsurfb.2025.115330

Xian Qiao Ding , Shreya Mukherjee , Luca Amagliani , Christophe Schmitt , Chueh Loo Poh

Plant proteins exhibit wide variability in emulsifying behavior due to differences in composition, molecular structure, and processing history. This study investigated the relationship between intrinsic physicochemical properties and emulsifying behavior of eleven commercial protein isolates and concentrates derived from bovine milk, soy, canola, faba bean and pea. Key properties including solubility, surface hydrophobicity (SH), molecular weight distribution (MWD), and zeta-potential of the protein ingredients were characterized and evaluated in relation to emulsion droplet size. Among these parameters, MWD showed a moderate correlation with emulsion droplet size (R² = 0.64), while SH and zeta potential exhibited no meaningful correlation (R² < 0.1). When MWD and SH were evaluated together, a clearer trend and separation emerged. Plant proteins with both high molecular weight and low surface hydrophobicity showed a tendency to form heavily flocculated emulsions. This flocculation likely resulted from slower interfacial adsorption and incomplete surface coverage, leading to droplet flocculation at early stages of emulsification. Under a specific set of formulation and processing conditions, emulsions prepared with alkaline-extracted pea and faba protein had smaller droplet size than their air-classified counterparts, likely due to their lower MWD and modified interfacial behavior induced by alkaline treatment. These findings show that the combination of MWD and SH could be useful in understanding the emulsifying behavior of commercial plant protein ingredients and helping in the screening and formulation of plant protein ingredients for food and beverage applications.

由于植物蛋白在组成、分子结构和加工历史上的差异，其乳化行为具有广泛的可变性。研究了从牛乳、大豆、菜籽油、蚕豆和豌豆中提取的11种商品分离蛋白和浓缩蛋白的内在理化性质与乳化行为的关系。主要性能包括溶解度、表面疏水性（SH）、分子量分布（MWD）和ζ电位，并与乳滴大小进行了表征和评价。其中，MWD与乳化液液滴大小有中等相关性（R²= 0.64），SH与zeta电位无显著相关性（R²< 0.1）。当MWD和SH一起评价时，趋势和分离更加明显。高分子量和低表面疏水性的植物蛋白容易形成重絮凝乳状体。这种絮凝可能是由于界面吸附速度较慢和表面覆盖不完全，导致乳化早期出现液滴絮凝。在特定的配方和加工条件下，用碱性提取的豌豆和蚕豆蛋白制备的乳状液的液滴尺寸比空气分类的乳状液小，这可能是由于它们的MWD较低，并且碱性处理导致了界面行为的改变。这些发现表明，MWD和SH的结合可用于了解商业植物蛋白成分的乳化行为，并有助于筛选和配制用于食品和饮料的植物蛋白成分。

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引用次数: 0

Preparation and characterization of hyaluronic acid nanoparticles for procyanidins hepatocellular and mitochondrial targeting delivery 原花青素肝细胞和线粒体靶向递送透明质酸纳米颗粒的制备和表征。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-28 DOI: 10.1016/j.colsurfb.2025.115334

Mengmeng Xue , Yanyan Li , Shanshan Tie , Yunling Zhang , Xue Zhang , Shichang Li , Jianrui Sun , Dongyang Zhu , Yuwan Li , Shaobin Gu

The purpose of this study was to design and fabricate hepatocyte and mitochondria dual-targeted nanoparticles for improving the stability and utilization of procyanidins (PC). PC nanoparticles were prepared by emulsion solvent evaporation method using (5-carboxypentyl) (triphenyl) phosphonium bromide modified PC as core materials, lactobionic acid and 3-amino phenylboronic acid modified hyaluronic acid as wall materials. The obtained nanoparticles were spherical, with small size, high encapsulation efficiency (> 80 %) and strong antioxidant capacity. Compared with free PC, the stability of loaded PC was improved under high temperature, UV radiation, long-term storage, freeze-thaw cycles, simulated digestion and other conditions. In vitro studies proved that nanoparticles had reactive oxygen species-responsive, hepatocyte and mitochondria dual targeting functional properties. In addition, dual-targeted PC nanoparticles could also inhibit the elevation of intracellular reactive oxygen species level and the decrease of mitochondrial membrane potential caused by high-fat. This work proposes a new strategy to deliver active compounds to hepatocytes and even mitochondria, which can provide new ideas for nutritional interventions in liver-related chronic diseases induced by high-fat diets.

本研究旨在设计和制备肝细胞和线粒体双靶向纳米颗粒，以提高原花青素（PC）的稳定性和利用率。以（5-羧基戊基）（三苯基）溴化磷改性聚乳酸为芯材，乳酸和3-氨基苯硼酸改性透明质酸为壁材，采用乳液溶剂蒸发法制备聚乳酸纳米颗粒。所制得的纳米颗粒呈球形，体积小，包封效率高（> 80 %），抗氧化能力强。与游离PC相比，加载PC在高温、紫外线辐射、长期储存、冻融循环、模拟消化等条件下的稳定性得到提高。体外研究证明，纳米颗粒具有活性氧物种响应、肝细胞和线粒体双重靶向功能特性。此外，双靶向PC纳米颗粒还能抑制高脂肪引起的细胞内活性氧水平升高和线粒体膜电位降低。本研究提出了一种将活性化合物输送到肝细胞甚至线粒体的新策略，可为高脂饮食引起的肝脏相关慢性疾病的营养干预提供新思路。

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引用次数: 0

Corrigendum to "Multifunctional mesoporous silica nanoparticles for pH-response and photothermy enhanced osteosarcoma therapy" [Colloids Surf. B Biointerfaces, 217 (2022) 112615/COLSUB-D-22-00508]. “用于ph响应和光热增强骨肉瘤治疗的多功能介孔二氧化硅纳米颗粒”的勘误表[Colloids Surf]。[j].中国生物医学工程学报，2015(5):387 - 391。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-28 DOI: 10.1016/j.colsurfb.2025.115323

Qinying Shi, Ying Lu, Guannan Zhang, Xin Yang, Rong Li, Guoliang Zhang, Xiudong Guo, Jianbo Song, Qin Ding

引用次数: 0

Exosome circ_0076611-mediated macrophage M2 polarization to improve triple-negative breast cancer metastasis and the intervention of baicalin nano prodrugs 外泌体circ_0076611介导的巨噬细胞M2极化改善三阴性乳腺癌转移及黄芩苷纳米前药的干预作用

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-28 DOI: 10.1016/j.colsurfb.2025.115333

Meng Lan , Xiaochun Lei , Yu Lin , Maciej Mazur , Jie Ning , Jaiwoo Lee , Li Bai , Honghui Gu , Qi Li , Lina Yang , Zhong Chen , Zhenjiang Yang , Detang Li , Yu Cai

Objective

M2 tumor-associated macrophages (TAMs) can promote metastasis of triple-negative breast cancer (TNBC). TNBC cells release exosomes to exchange information. By doing so, they reprogram the phenotype of TAMs. TRAF3IP2 is a key factor mediating the metastasis and is highly expressed in TNBC; however, it is still unclear whether it has an impact on TAMs. This study explored the mechanism of the interaction between TNBC and TAMs, and evaluated a novel nanotherapy strategy to intervene in this process.

Methods

RT-qPCR was used to quantify TRAF3IP2 and circ_0076611 expression. Flow cytometry and RT-qPCR were used to analyze M2 macrophage polarization. ELISA was used to quantify immunosuppressive cytokine secretion. Transwell invasion and western blot were used to evaluate the aggressiveness of TNBC cells. The baicalin nano prodrug was developed, and its binding to TRAF3IP2 was validated by molecular docking. A mouse tumor model was established to study the mechanism of TNBC metastasis in vivo.

Results

TRAF3IP2 promoted M2 polarization in macrophages, thereby enhancing TNBC invasion. Exosome circ_0076611 mediated this induction. Molecular docking and isothermal titration calorimetry confirmed baicalin binding to TRAF3IP2. The prepared baicalin nano prodrug inhibited exosome circ_0076611 through TRAF3IP2 and reduced M2 macrophage infiltration. Consequently, it inhibited TNBC growth and lung metastasis.

Conclusion

The induction of M2 macrophage polarization by exosome circ_0076611 was regulated by TRAF3IP2, which was a key driver of TNBC metastasis. The constructed baicalin nano prodrug based on this provided an intervention strategy for targeted nanotherapy.

目的：M2肿瘤相关巨噬细胞（tam）可促进三阴性乳腺癌（TNBC）的转移。TNBC细胞释放外泌体来交换信息。通过这样做，他们重新编程了tam的表型。TRAF3IP2是介导TNBC转移的关键因子，在TNBC中高表达；然而，目前尚不清楚它是否会对tam产生影响。本研究探讨了TNBC和TAMs之间相互作用的机制，并评估了一种新的纳米治疗策略来干预这一过程。方法：采用RT-qPCR法定量TRAF3IP2和circ_0076611的表达。流式细胞术和RT-qPCR检测M2巨噬细胞极化情况。ELISA法定量测定免疫抑制细胞因子的分泌。采用Transwell侵袭法和western blot法评价TNBC细胞的侵袭性。制备了黄芩苷纳米前药，并通过分子对接验证了其与TRAF3IP2的结合。建立小鼠肿瘤模型，研究TNBC在体内转移的机制。结果：TRAF3IP2促进巨噬细胞M2极化，从而增强TNBC侵袭。外泌体circ_0076611介导了这种诱导。分子对接和等温滴定量热法证实黄芩苷与TRAF3IP2结合。制备的黄芩苷纳米前药通过TRAF3IP2抑制外泌体circ_0076611，减少M2巨噬细胞浸润。因此，它抑制TNBC的生长和肺转移。结论：外泌体circ_0076611诱导M2巨噬细胞极化受TRAF3IP2调控，是TNBC转移的关键驱动因子。在此基础上构建的黄芩苷纳米前药为靶向纳米治疗提供了一种干预策略。

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引用次数: 0

Protein papers from microfibrillated silk for biomedical membrane applications 生物医学膜用微纤丝蛋白纸

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-28 DOI: 10.1016/j.colsurfb.2025.115329

Limna Suja Shaji , Saumya Saji Kochumoni , Benjamin J. Allardyce , David A.V. Morton , Warren Batchelor , Naghmeh Nasiri , Rangam Rajkhowa

Microfibrillated silk (MFS) is an emerging class of silk materials produced by directly exfoliating silk fibres, offering a top-down alternative to traditional regenerated silk processing methods. They have the potential to be used as biomaterials, particularly in tissue engineering and regenerative medicine. This study used a unique tuneable top-down approach to produce different MFS suspensions. These were then fabricated into protein papers using two scalable methods, casting and vacuum filtration, to examine how processing and fabrication together influenced the final material properties and associated cellular responses. MFS suspensions were prepared under three processing levels using mechanical processing alone or combined with acid pre-treatment with each level yielding increasingly finer fibrils. The level of fibrillation significantly affected fibre morphology and mechanical strength, while the fabrication method mainly influenced surface roughness and bilayer characteristics. Papers made with mechanical processing alone had the highest strength. Cast papers revealed a surface difference, with a rough top surface and smooth bottom surface, while vacuum-filtered papers had uniform roughness on both sides. These surface differences, along with the degree of fibrillation, impacted cell attachment and organization, with cast papers from acid-pretreated and shear-homogenised MFS showing the best biological outcomes. Controlling MFS processing and assembly techniques to form papers enables the design of silk-based materials tailored for various biomedical applications.

微纤丝（MFS）是一种新兴的蚕丝材料，由直接去角质的蚕丝纤维生产，提供了一种自上而下的替代传统的再生蚕丝加工方法。它们具有用作生物材料的潜力，特别是在组织工程和再生医学方面。本研究使用了一种独特的可调自顶向下的方法来产生不同的MFS悬架。然后使用两种可扩展的方法，铸造和真空过滤，将这些材料制成蛋白质纸，以研究加工和制造如何共同影响最终的材料特性和相关的细胞反应。MFS悬浮液的制备分为三个加工水平，分别采用单独机械加工或与酸预处理相结合的方法，每个加工水平的原纤维越来越细。纤维的纤颤程度显著影响纤维的形态和机械强度，而制备方法主要影响表面粗糙度和双层特性。单靠机械加工的纸张强度最高。铸纸的表面存在差异，上表面粗糙，下表面光滑，而真空过滤纸的两面粗糙度均匀。这些表面差异以及纤颤的程度影响了细胞的附着和组织，酸预处理和剪切均质MFS的铸模纸显示出最佳的生物学效果。控制MFS加工和组装技术以形成纸张，可以设计适合各种生物医学应用的丝绸基材料。

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引用次数: 0

A three-stage differentiation method for generating induced mesenchymal stem cells from human pluripotent stem cells via gelatin-based screening 基于明胶筛选的人多能干细胞诱导间充质干细胞的三期分化方法

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-28 DOI: 10.1016/j.colsurfb.2025.115324

Linli Wang , Qiang Li , Yuanyuan Li , Luyao Chen , Jianguo Yang , Peizheng Wang , Tiancheng Zhou , Yu Liu , Yuehua Chen , Hong Wang

Abstract

Human pluripotent stem cell (hPSC)-derived induced mesenchymal stem cells (iMSCs) offer advantages over tissue-derived MSCs in their in unlimited expansion, minimal batch-to-batch variation, controllable pathogen status, and large-scale production potential. Yet traditional iMSC differentiation methods face limitations that necessitate a more efficient approach. Based on the classic TGF-β inhibitor (SB431542) induction system, this study established a three-stage iMSC differentiation method: 10-day Laminin-521/Gelatin induction, 11-day screening on 0.1 % gelatin-coated dishes (enriching precursors via differential cell-gelatin adhesion), and 3–5-day amplification. A xeno-free system was established. Flow cytometry, trilineage differentiation, RNA sequencing, karyotype analysis, and nude mouse tumorigenicity tests validated iMSC quality. This straightforward, cost-effective method (≈￥2000–3000 for 1 ×10⁸ P0 iMSCs) yields high-purity iMSCs with strong proliferation, tissue-derived MSC-like transcriptomes, and enhanced bone repair capacity in a mouse femoral fracture model. It also further implicates the gelatin DGEA motif (which mimics gelatin’s effect) as key for iMSC differentiation, addressing classical iMSC industrialization issues and informing clinical translation.

人多能干细胞（hPSC）衍生的诱导间充质干细胞（iMSCs）具有无限扩增、批间变异最小、病原体状态可控和大规模生产潜力等优势。然而，传统的iMSC分化方法面临局限性，需要一种更有效的方法。本研究以经典的TGF-β抑制剂（SB431542）诱导体系为基础，建立了3阶段的iMSC分化方法：10天Laminin-521/明胶诱导，11天在0.1 %明胶包被皿上筛选（通过差异细胞-明胶粘附富集前体），3 -5天扩增。建立了无xeno体系。流式细胞术、三龄分化、RNA测序、核型分析和裸鼠致瘤性试验验证了iMSC的质量。这种简单、经济的方法（1 ×10⁸P0 iMSCs≈￥2000-3000）在小鼠股骨骨折模型中获得了高纯度的具有强增殖、组织来源的msc样转录组和增强的骨修复能力的iMSCs。它还进一步暗示明胶DGEA基元（模仿明胶的效果）是iMSC分化的关键，解决经典的iMSC工业化问题并为临床翻译提供信息。

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引用次数: 0

Dissecting the atomic effects of S305 phosphorylation on the aggregation of the core tau peptides of the first intermediate filament 解剖S305磷酸化对第一中间丝核心tau肽聚集的原子效应。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-27 DOI: 10.1016/j.colsurfb.2025.115326

Jiaxing Tang , Dushuo Feng , Lulu Guan , Zhengdong Xu , Yu Zou

Tauopathies, containing aging-related Alzheimer’s disease (AD) and contact sports-related chronic traumatic encephalopathy (CTE), etc., are neuropathologically characteristic of the tau protein aggregates in the brain. Targeting the oligomeric species formed in the route of tau fibrilization has been considered a promising therapeutic approach to prevent or treat tauopathies. In vitro experiment reported that S305 phosphorylation (Pho-S305) exerted a protective role in tau aggregation. However, the atomic effect and molecular mechanisms of Pho-S305 on tau aggregation are largely elusive. In this study, we performed replica exchange molecular dynamics (REMD) and classical molecular dynamics (MD) simulations, with a total simulation time of 57 μs, on tau peptides without and with Pho-S305. The protein model was a novel tau fragment (G302-S316), constituting the ordered core of a first intermediate amyloid (FIA) filament of AD- and CTE-specific tau filament. The REMD results revealed that Pho-S305 suppressed the β-sheet formation and weakened the peptide-peptide interaction, thus inhibiting the aggregation of the peptides. Additional MD simulations indicated that the oligomerization dynamics of the peptides were disturbed by Pho-S305. Our findings excavate the mechanistic information underlying the phosphorylation-induced inhibitive effects on tau_302–316 aggregation, which may provide potential useful clues for the development of therapeutic avenues for tauopathies.

tau病，包括衰老相关的阿尔茨海默病（AD）和接触性运动相关的慢性创伤性脑病（CTE）等，是tau蛋白聚集在大脑中的神经病理学特征。靶向在tau蛋白纤化过程中形成的寡聚物已被认为是预防或治疗tau病变的一种有前途的治疗方法。体外实验报道S305磷酸化（phoo -S305）在tau聚集中发挥保护作用。然而，phoo - s305对tau聚集的原子效应和分子机制在很大程度上是未知的。在这项研究中，我们对不含和含phoo - s305的tau肽进行了复制交换分子动力学（REMD）和经典分子动力学（MD）模拟，总模拟时间为57 μs。该蛋白模型是一个新的tau片段（G302-S316），构成AD-和cte特异性tau丝的第一中间淀粉样蛋白（FIA）丝的有序核心。REMD结果显示，Pho-S305抑制β-sheet的形成，减弱肽-肽相互作用，从而抑制肽的聚集。另外的MD模拟表明，phoo - s305干扰了肽的寡聚动力学。我们的发现挖掘了磷酸化诱导的对tau302-316聚集抑制作用的机制信息，这可能为开发tau病的治疗途径提供潜在的有用线索。

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引用次数: 0

Chiral biomaterials for promoting wound healing: Fabrication strategies, therapeutic applications, and future prospects 促进伤口愈合的手性生物材料：制造策略、治疗应用和未来展望

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-27 DOI: 10.1016/j.colsurfb.2025.115328

Yongbin Duan , Siyu Chen , Yan Wang , Zhongshuang Liu , Lansheng Wei , Bichong Luo , Wenjing Liu , Hai Gao

The skin is highly susceptible to damage from various factors of daily life, which can cause wounds to form. Accelerated wound healing is important for enhancing patient quality of life and reducing healthcare burdens. Chiral biomaterials (CBMs), with non-superimposable mirror-image molecular structures, have emerged as promising candidates for enhancing wound healing due to their unique biointeractions. However, there are few comprehensive reviews on the role of CBMs across different wound healing phases. This review first introduces common methods for preparing CBMs, including ligand conjugation, self-assembly, and template-assisted synthesis. The effects of CBMs on different aspects of wound healing are summarized, including hemostasis, antibacterial activity, inflammatory regulation, the proliferation phase, the remodeling phase, and wound healing monitoring, and the underlying mechanisms exerted by CBMs are elucidated. Furthermore, the biosafety of CBMs is critically discussed. Finally, the review highlights current challenges and future directions in the field and proposes potential strategies to address current limitations. By providing a thorough analysis, this work aims to offer valuable insights and inspire innovative approaches for research on CBMs in wound healing.

皮肤很容易受到日常生活中各种因素的伤害，这可能导致伤口的形成。加速伤口愈合对于提高患者的生活质量和减轻医疗负担非常重要。手性生物材料（CBMs）具有不可重叠的镜像分子结构，由于其独特的生物相互作用，已成为促进伤口愈合的有希望的候选者。然而，很少有关于CBMs在不同伤口愈合阶段的作用的全面综述。本文首先介绍了制备CBMs的常用方法，包括配体偶联、自组装和模板辅助合成。综述了CBMs在创面愈合过程中止血、抗菌、炎症调节、增殖期、重塑期、创面愈合监测等方面的作用，并阐述了CBMs的作用机制。此外，对CBMs的生物安全性进行了批判性的讨论。最后，该综述强调了该领域当前的挑战和未来的方向，并提出了解决当前限制的潜在战略。通过深入的分析，本研究旨在为伤口愈合中CBMs的研究提供有价值的见解和启发创新的方法。

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引用次数: 0

Chitosan-based antibacterial hemostatic sponge for uncontrollable tooth extraction bleeding in anticoagulated patients 壳聚糖抗菌止血海绵用于抗凝患者拔牙不可控出血。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-27 DOI: 10.1016/j.colsurfb.2025.115325

Rongxia Tang , Lu Zheng , Shangfei Chi , Lu Wei , Xiaoying Wang , Xiao Ma

Anticoagulant therapy substantially increases the risk of post-extraction hemorrhage by impairing hemostasis. In this study, we designed a quaternized carboxymethyl chitosan/polydopamine composite hemostatic sponge (QCD) that combines mechanical robustness and biocompatibility. Compared with the Gel group, QCD exhibits markedly enhanced in vitro hemostatic and antibacterial performance; the quaternary ammonium modification confers potent antibacterial activity tailored to the complex oral environment and mitigates infection risk. In vivo, tooth extraction test in anticoagulated rats showed that QCD (blood loss 0.011 ± 0.001 g, hemostasis time 59.667 ± 4.163 s) significantly outperformed a commercial gelatin sponge (0.019 ± 0.001 g, 87.000 ± 4.082 s) in hemostasis. This superiority was attributable to its rapid blood absorption and enhanced adhesion of blood cells and platelets, which together promoted clot formation. These results suggest that QCD may serve as a practical and promising approach for managing post-extraction hemorrhage in anticoagulated patients.

抗凝治疗因妨碍止血而大大增加了拔牙后出血的风险。在这项研究中，我们设计了一种季铵盐化羧甲基壳聚糖/聚多巴胺复合止血海绵（QCD），它结合了机械坚固性和生物相容性。与凝胶组比较，中药复方清液明显增强体外止血和抗菌性能；季铵改性赋予了针对复杂口腔环境的有效抗菌活性，并减轻了感染风险。体内拔牙抗凝大鼠实验结果显示，QCD的止血效果（出血量0.011 ±0.001 g，止血时间59.667 ±4.163 s）明显优于商用明胶海绵（0.019 ±0.001 g, 87.000 ±4.082 s）。这种优势是由于其快速的血液吸收和增强血细胞和血小板的粘附，两者共同促进了凝块的形成。这些结果表明，QCD可能作为一种实用和有前途的方法来处理抗凝患者拔牙后出血。

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引用次数: 0

Use of hyperspectral imaging for the early detection of foodborne pathogens 高光谱成像在食源性病原体早期检测中的应用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-26 DOI: 10.1016/j.colsurfb.2025.115315

Ramalakshmi Alaguthevar , Akila Duraisamy , Balakrishnan Murugesan , Iniyakumar Muniraj , Ajahar Khan , Jong-Whan Rhim

Pathogenic food microbes pose a serious threat to global health, leading to widespread disease outbreaks, economic losses, and disruptions in the food processing and distribution chain. Current detection methods for infectious agents, including conventional culturing and genotypic techniques, are accurate but often face limitations such as costly equipment, the need for specialized personnel, and lengthy analysis times. Hyperspectral imaging (HSI) has emerged as a promising alternative that provides a fast, non-invasive, and high-throughput approach for early identification of pathogenic food microbes. HSI combines spatial and spectral characterization to deliver information across a broad wavelength range. This technology enables the recognition and identification of microbial agents by analyzing their unique spectral fingerprints, which reflect the biochemical and physical properties of bacteria, fungi, and viruses in various food matrices, including raw produce, animal protein, and milk-based foods. A typical HSI system can acquire a complete spectral cube in less than a minute per sample, capturing 100–300 contiguous spectral bands across the visible to near-infrared range, thus enabling rapid and comprehensive microbial screening. This review highlights several foodborne pathogens and the strong applicability of HSI for their identification. Additionally, it discusses recent trends, suggests future directions, and emphasizes AI adoption along with the development of portable HSI devices for on-site food safety assessment.

致病性食品微生物对全球健康构成严重威胁，导致广泛的疾病暴发、经济损失以及食品加工和分销链中断。目前的传染病检测方法，包括传统的培养和基因型技术，是准确的，但往往面临诸如昂贵的设备、对专业人员的需求和冗长的分析时间等限制。高光谱成像（HSI）已成为一种有前途的替代方法，为早期鉴定致病性食品微生物提供了一种快速、非侵入性和高通量的方法。HSI结合了空间和光谱特性，在广泛的波长范围内提供信息。该技术通过分析其独特的光谱指纹图谱来识别和鉴定微生物制剂，这些指纹图谱反映了各种食品基质（包括生农产品、动物蛋白和乳基食品）中细菌、真菌和病毒的生化和物理特性。典型的HSI系统可以在不到一分钟的时间内获得每个样品的完整光谱立方体，在可见光到近红外范围内捕获100-300个连续光谱带，从而实现快速全面的微生物筛选。这篇综述强调了几种食源性病原体和强适用性的HSI鉴定。此外，它还讨论了最近的趋势，提出了未来的方向，并强调了人工智能的采用以及用于现场食品安全评估的便携式HSI设备的发展。

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引用次数: 0