Colloids and Surfaces B: Biointerfaces最新文献_第5页

Correlation between oral disease and neurodegenerative disorders: Role of biological proteins for the modulation of oral-brain axis and gut-brain axis 口腔疾病与神经退行性疾病的相关性：生物蛋白在口脑轴和肠脑轴调节中的作用

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-27 DOI: 10.1016/j.colsurfb.2026.115480

Suraj Kumar , Saurabh Srivastava , Ching Siang Tan , Mohammed Abohashrh , Rishabha Malviya

Biological proteins play a crucial role at the intersection of oral health and neuroscience, offering promising opportunities for improved diagnosis, prevention, and treatment. This review highlights the molecular, inflammatory, and biochemical pathways linking oral diseases, particularly periodontal disease and microbial dysbiosis, with neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease. Key inflammatory, neuroprotective, and tissue-repair proteins play a crucial role in maintaining both oral integrity and neural function. Advances in proteomics and molecular imaging have clarified how protein misfolding, aggregation, and immune responses drive neuroinflammation and cognitive decline. Emerging therapies include protein-based biomaterials, such as hydrogels, nanocarriers, and protein–polymer hybrids, for delivering neuroprotective and regenerative agents through oral and nasal routes. Early diagnosis is being transformed by salivary proteomics and transcriptomics, enabling non-invasive detection of neurodegenerative biomarkers. Host-defense peptides and antimicrobial proteins also show promise in controlling oral infections that may exacerbate brain inflammation. Integrating oral biology, biomaterials science, and neuroscience is accelerating clinical translation through the development of innovative scaffolds and smart delivery systems. Despite challenges in biomarker validation and clinical application, advances in artificial intelligence, bioinformatics, and protein engineering are driving the future of personalized regenerative and preventive medicine. Overall, biological proteins provide a critical molecular link between oral and neural health, paving the way for novel non-invasive diagnostic and therapeutic strategies.

生物蛋白在口腔健康和神经科学的交叉领域发挥着至关重要的作用，为改进诊断、预防和治疗提供了有希望的机会。这篇综述强调了口腔疾病，特别是牙周病和微生物生态失调，与神经退行性疾病如阿尔茨海默病和帕金森病之间的分子、炎症和生化途径。关键的炎症、神经保护和组织修复蛋白在维持口腔完整性和神经功能方面起着至关重要的作用。蛋白质组学和分子成像的进展已经阐明了蛋白质错误折叠、聚集和免疫反应如何驱动神经炎症和认知能力下降。新兴疗法包括基于蛋白质的生物材料，如水凝胶、纳米载体和蛋白质-聚合物混合物，用于通过口服和鼻腔途径输送神经保护和再生剂。唾液蛋白质组学和转录组学正在改变早期诊断，使神经退行性生物标志物的非侵入性检测成为可能。宿主防御肽和抗菌蛋白在控制可能加剧脑部炎症的口腔感染方面也显示出希望。口腔生物学、生物材料科学和神经科学的整合正在通过开发创新支架和智能给药系统加速临床转化。尽管在生物标志物验证和临床应用方面存在挑战，但人工智能、生物信息学和蛋白质工程的进步正在推动个性化再生和预防医学的未来。总的来说，生物蛋白在口腔和神经健康之间提供了关键的分子联系，为新的非侵入性诊断和治疗策略铺平了道路。

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引用次数: 0

CP-31398 restored the functional condensates of R175H p53 by stabilizing the zinc-binding domain and 251–258 segment CP-31398通过稳定锌结合结构域和251-258段恢复了R175H p53的功能缩合物

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-26 DOI: 10.1016/j.colsurfb.2026.115474

Yang Liu , Yuan Liu , Chang Xu , Fangming Jiang , Xiaorong Yang

Tumor suppressor p53 formed the droplets with the solidification tendency. Mutations in p53 could accelerate the aggregation of droplets, resulting in p53 to lose the function and/or to gain the oncogenic activity. In this study, the effects of CP-31398 on the phase behaviors of p53 mutants were explored. The results revealed that CP-31398 could inhibit the pathological aggregation of R175H p53, restored the interaction between R175H p53 and specific DNA, and promoted the formation of functional droplets. For R248W p53, CP-31398 could regulate the phase behavior but not restore the formation of functional condensates. Molecular dynamics simulations showed that CP-31398 enhanced the structural stability of R175H p53 by stabilizing the zinc-binding domain and 251–258 segment. These findings provided new insights into the molecular basis that CP-31398 restored the liquid-liquid phase separation of p53 mutant, and could offer the novel therapeutic strategy for cancers with p53 mutant.

肿瘤抑制因子p53形成具有凝固倾向的液滴。p53突变可加速液滴聚集，导致p53失去功能和/或获得致癌活性。本研究探讨了CP-31398对p53突变体相行为的影响。结果显示，CP-31398可以抑制R175H p53的病理聚集，恢复R175H p53与特异性DNA的相互作用，促进功能液滴的形成。对于R248W p53， CP-31398可以调节相行为，但不能恢复功能凝聚物的形成。分子动力学模拟表明，CP-31398通过稳定锌结合结构域和251-258片段增强了R175H p53的结构稳定性。这些发现为CP-31398恢复p53突变体液液相分离的分子基础提供了新的见解，并为p53突变体癌症的治疗提供了新的策略。

引用次数: 0

Exploration of sub-cellular responses for the evaluation of the laser mediated tumor ablation via Raman spectroscopic platform using green synthesized gold nanostars 利用绿色合成金纳米星在拉曼光谱平台上探索激光介导肿瘤消融的亚细胞反应

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-25 DOI: 10.1016/j.colsurfb.2026.115472

BS Unnikrishnan , GU Preethi , PT Sujai , Kaustabh Kumar Maiti , TT Sreelekha

Anisotropic gold nanoparticles with surface plasmon resonance (SPR) can generate heat upon exposure to laser light, which can then be utilised for photothermal therapy (PTT) in cancer treatment. In this study, we report the use of doxorubicin (DOX)-loaded immunomodulatory polysaccharide (PST001) coated anisotropic gold nanostars (AuNS@PST) as a theranostic carrier for photothermal-chemotherapy. AuNS@PST were prepared by green synthesis followed by doxorubicin encapsulation. The synthesized particles were characterized using UV–vis spectroscopy, DLS, FTIR and TEM. The heat dissipation of these nanoparticles was monitored in aqueous phantoms using 635 nm laser sources, which indicated the thermal rise from ambient temperature. The in vitro cytotoxicity analysis of AuNS@PST was done using the MTT assay in A549 cells. Significantly lower IC₅₀ value was observed for cells treated with DOX-loaded PST AuNSs when compared to DOX-alone treated cells. Similarly, DOX-loaded AuNS@PST had efficient photothermal-induced apoptosis exerted by the laser-irradiated nanoparticles. Changes in protein degradation and DNA fragmentation at the subcellular levels were observed in the Raman spectrum. Although supplementary perspectives are required in the proper investigation of laser-mediated cell death in cancer tissues, the current study discloses the emerging methodology to track apoptotic events in cancer tissues using the Raman scattering platform, even at the sub-cellular level.

具有表面等离子体共振（SPR）的各向异性金纳米粒子可以在激光照射下产生热量，然后可以用于光热治疗（PTT）癌症治疗。在这项研究中，我们报道了使用负载阿霉素（DOX）的免疫调节多糖（PST001）包被各向异性金纳米星（AuNS@PST）作为光热化疗的治疗载体。AuNS@PST为绿色合成工艺，经阿霉素包封制备。采用紫外可见光谱、DLS、FTIR和TEM对合成的颗粒进行了表征。利用635 nm激光源对纳米颗粒在水相模型中的散热进行了监测，结果表明纳米颗粒的热升高与环境温度有关。采用MTT法对A549细胞进行AuNS@PST体外细胞毒性分析。与单独处理dox的细胞相比，负载dox的PST AuNSs处理细胞的IC50值显着降低。同样，负载dox的AuNS@PST具有有效的光热诱导的凋亡，由激光照射的纳米颗粒施加。在拉曼光谱中观察到亚细胞水平上蛋白质降解和DNA断裂的变化。虽然在癌症组织中激光介导的细胞死亡的适当研究中需要补充的观点，但目前的研究揭示了使用拉曼散射平台甚至在亚细胞水平上跟踪癌症组织中凋亡事件的新兴方法。

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引用次数: 0

Biomimetic red blood cell membrane–coated cerium metal–organic framework for multi-target synergistic therapy of Alzheimer’s disease 仿生红细胞膜包覆金属铈-有机框架多靶点协同治疗阿尔茨海默病

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-23 DOI: 10.1016/j.colsurfb.2026.115464

Fengmei Yang , Yutong Chen , Yujiao Yan , Ruixin Zhao , Liran Deng , Die Tian , Meng Xie

Pathological events in Alzheimer’s disease (AD) typically involve β-amyloid (Aβ) plaque deposition, metal ion dysregulation, oxidative stress elevation, and chronic neuroinflammation, making single-target therapies unsatisfactory. Here, we first report a biomimetic nanoplatform based on red blood cell membrane–coated cerium metal–organic frameworks (Ce-MOF-RBC) that enables multi-target synergistic intervention against AD. The Ce-MOF core exhibits potent antioxidant activity, efficiently scavenging reactive oxygen species (ROS) and restoring mitochondrial membrane potential, while its carboxylate ligands chelate Cu^2 + with high efficiency (49.26 %) to inhibit Cu²⁺-induced Aβ fibrillation and disassemble preformed fibrils. Ce-MOF-RBC further modulates microglial phenotype, enhancing Aβ phagocytosis and reducing neuroinflammation. Importantly, RBC membrane functionalization markedly improves biological performance by prolonging systemic circulation, enhancing blood–brain barrier (BBB) penetration, and leveraging its intrinsic affinity for Aβ peptides to enrich Aβ. In vivo fluorescence imaging and brain cryosections showed that Ce-MOF-RBC achieved robust accumulation in the cortex and hippocampus, with brain fluorescence intensities 27.33-fold higher than free DiD. In the C. elegans AD model, Ce-MOF-RBC reduced Aβ plaque fluorescence by 32.54 %, lowered ROS levels by 45.72 %, improved chemotaxis performance (chemotaxis index increased from 34.24 % to 68.34 %), and delayed paralysis onset from 10 h to 15 h, demonstrating significant rescue of cognitive and motor deficits. In summary, these findings highlight the first demonstration of a small-sized, biomimetic Ce-MOF-RBC nanoplatform that integrates antioxidant, metal-chelating, anti-aggregation, and immunomodulatory functions, offering a promising strategy for comprehensive AD therapy.

阿尔茨海默病（AD）的病理事件通常包括β-淀粉样蛋白（Aβ）斑块沉积、金属离子失调、氧化应激升高和慢性神经炎症，这使得单靶点治疗不理想。在这里，我们首次报道了一种基于红细胞膜涂层铈金属有机框架（Ce-MOF-RBC）的仿生纳米平台，该平台可以实现对AD的多靶点协同干预。Ce-MOF核心具有强大的抗氧化活性，可有效清除活性氧（ROS）并恢复线粒体膜电位，而其羧酸配体可高效（49.26 %）螯合Cu2 +，抑制Cu2+诱导的Aβ纤颤和分解预形成的原纤维。Ce-MOF-RBC进一步调节小胶质细胞表型，增强Aβ吞噬和减少神经炎症。重要的是，红细胞膜功能化通过延长体循环，增强血脑屏障（BBB）渗透，并利用其对Aβ肽的内在亲和力来丰富Aβ，显著提高了生物学性能。体内荧光成像和脑冷冻切片显示，Ce-MOF-RBC在皮质和海马区有强大的积累，脑荧光强度比游离DiD高27.33倍。在秀丽隐杆线虫AD模型中，Ce-MOF-RBC降低了Aβ斑块荧光32.54 %，降低了ROS水平45.72 %，改善了趋化性表现（趋化性指数从34.24 %增加到68.34 %），并将麻痹发作时间从10 h推迟到15 h，显示出对认知和运动缺陷的显著缓解。总之，这些发现强调了一个小尺寸、仿生Ce-MOF-RBC纳米平台的首次展示，该平台集抗氧化、金属螯合、抗聚集和免疫调节功能于一体，为全面治疗阿尔茨海默病提供了一个有希望的策略。

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引用次数: 0

Rhein-modified hemodialysis membranes suppress red blood cell fatigue and thrombosis. 莱茵改性血液透析膜抑制红细胞疲劳和血栓形成。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-23 DOI: 10.1016/j.colsurfb.2026.115469

Xiao Fu, Ting Lei, Linxin Liu, Yanfeng Liu

Dialysis membrane-related hemolysis (DMH) is an important factor influencing blood purification efficacy in critically ill patients. However, the pre-hemolysis fatigue process of RBCs and whether RBC fatigue can induce thrombus formation remains largely unexplored. In this study, rhein-modified polysulfone (PSf) hemodialysis membranes (RH membranes) were fabricated, and their impact on RBC fatigue and thrombus formation was investigated. In this study, the RH membranes demonstrated potent physicochemical properties and hemodialysis performance, effectively inhibiting RBC fatigue and RMPs formation, and alleviating hemoglobin (Hb)-mediated sublethal damage. Furthermore, we showed that RH membranes exhibited antithrombotic efficacy by diminishing RMPs formation and subsequently inhibiting activation of predominant coagulation pathways and key coagulation factors. In the murine model of acute hemolysis, the RH membranes mitigated DMH in vivo and provided anticoagulation and organ-protective effects. In conclusion, RH membranes are a promising option for hemodialysis treatment in critically ill patients.

透析膜相关性溶血（DMH）是影响危重患者血液净化效果的重要因素。然而，红细胞的溶血前疲劳过程以及红细胞疲劳是否能诱导血栓形成仍未得到充分研究。本研究制备了大黄素修饰聚砜（PSf）血液透析膜（RH膜），并研究了其对红细胞疲劳和血栓形成的影响。在这项研究中，RH膜表现出强大的物理化学性质和血液透析性能，有效抑制红细胞疲劳和RMPs的形成，减轻血红蛋白（Hb）介导的亚致死损伤。此外，我们发现RH膜通过减少RMPs的形成并随后抑制主要凝血途径和关键凝血因子的激活来表现出抗血栓的功效。在小鼠急性溶血模型中，RH膜在体内减轻DMH，并具有抗凝和器官保护作用。总之，RH膜是危重患者血液透析治疗的一个有希望的选择。

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引用次数: 0

Biosynthetic fingerprints and strain-specific characterization of bacterial cellulose from vinegar-derived Komagataeibacter spp. 醋源Komagataeibacter spp细菌纤维素的生物合成指纹图谱及菌株特异性表征。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-22 DOI: 10.1016/j.colsurfb.2026.115453

Elif Naz Gürsoy, M. Burcu Kulahci, Kubra Sener, K. Barbaros Balabanli, Sule Coskun Cevher

Bacterial cellulose (BC) is a nanostructured biopolymer renowned for its high crystallinity, exceptional water-holding capacity, and biocompatibility. While interspecies differences in BC properties are documented, comprehensive insights into strain-level biosynthetic variability within and across Komagataeibacter species remain limited. In this study, ten BC-producing strains were isolated from vinegar samples of diverse botanical origins and identified via 16S rRNA sequencing as belonging to three species: K. europaeus, K. medellinensis, and K. xylinus. Despite the limited taxonomic diversity, the isolates exhibited ten distinct “biosynthetic fingerprints,” as evidenced by pronounced variations in production yield, micro- and nanostructural morphology (FE-SEM), chemical functionality (FTIR), crystallinity (XRD), thermal stability (TGA), and water-holding capacity (WHC). Quantitatively, BC yields ranged from 2.10 to 3.01 g L⁻¹ , crystallinity indices from 34.5 % to 65.6 %, DTG_max from 337.4 °C to 372.1 °C, and WHC from 87.3 % to 95.7 %. Interestingly, some low-crystallinity samples demonstrated higher thermal stability, indicating that microstructural organization and fibril network compactness contribute significantly to thermal resistance beyond crystallinity alone. These findings establish that vinegar-derived Komagataeibacter strains harbor substantial strain-specific structural and functional heterogeneity, underscoring the necessity of strain-level selection and characterization when tailoring BC for advanced biomedical and industrial applications.

细菌纤维素（BC）是一种纳米结构的生物聚合物，以其高结晶度、优异的保水能力和生物相容性而闻名。虽然物种间BC特性的差异已被记录，但对Komagataeibacter物种内和物种间菌株水平生物合成变异性的全面了解仍然有限。本研究从不同植物来源的食醋样品中分离到10株产bc菌株，通过16S rRNA测序鉴定为3个种：K. europaeus、K. medellinensis和K. xylinus。尽管分类多样性有限，但分离物表现出10种不同的“生物合成指纹”，这可以通过产量、微观和纳米结构形态（FE-SEM）、化学功能（FTIR）、结晶度（XRD）、热稳定性（TGA）和保水能力（WHC）的显著变化来证明。BC产率从2.10到3.01 g L⁻¹ ，结晶度指数从34.5% %到65.6 %，DTG_max从337.4 °C到372.1 °C， WHC从87.3% %到95.7% %。有趣的是，一些低结晶度的样品表现出更高的热稳定性，这表明微观结构组织和纤维网络紧密性对热阻的影响远远超过结晶度。这些发现表明，食醋衍生的komagataeibacterium菌株具有大量菌株特异性结构和功能异质性，强调了在为先进的生物医学和工业应用定制BC时，菌株水平选择和表征的必要性。

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引用次数: 0

Dynamic linking bone ECM-mimic hydrogel for anti-inflammatory therapy of cranial defect 动态连接骨ecm模拟水凝胶抗炎治疗颅骨缺损

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-22 DOI: 10.1016/j.colsurfb.2026.115462

Tao Ge, Hongcai Wang, Mengmeng Qiu, Kuan Feng, Taotao Shi, Jia Li, Shidi Yang, Maosong Chen

Cranial defect repair is frequently hindered by limited intrinsic regenerative capacity, infection risks, and chronic inflammation, whereas conventional grafts and inert implants often suffer from poor host integration. Herein, we report an extracellular matrix–mimicking hydrogel that synchronizes angiogenesis, osteogenesis, and immunomodulation through dynamic thiol–disulfide chemistry and sacrificial prevascularization. Hyaluronic acid functionalized with L-cysteine ethyl ester (HACys) was crosslinked with allicin to yield a viscoadaptive, stress-relaxing network, within which type I collagen was extruded into VEGF-loaded threads to serve as sacrificial templates (HACys-VEGF@Coll-A). Upon enzymatic degradation, these threads generated VEGF-lined microchannels designed to guide rapid vascular ingress. The composite demonstrated excellent cytocompatibility with BMSCs and selectively enhanced HUVEC viability and spreading. Furthermore, VEGF-presenting constructs significantly promoted endothelial tube formation and migration while upregulating VEGF mRNA, confirming preserved bioactivity. In BMSCs, VEGF-containing hydrogels increased alkaline phosphatase activity and mineral deposition, concomitant with the upregulation of osteogenic genes (ALP, COL1, RUNX2, OCN). Notably, macrophages shifted from a CD86high/CD206low toward a CD86low/CD206high phenotype with decreased TNF-α and increased IL-10 secretion, indicating the establishment of a pro-resolution immune microenvironment. Collectively, HACys-VEGF@Coll-A forms a dynamic, remodelable scaffold that integrates preformed vascular conduits while supporting osteogenesis and tempering inflammation, thereby addressing major barriers to cranial defect repair and warranting further in vivo evaluation of release kinetics, channel architecture, and mechanics.

颅骨缺损修复经常受到有限的内在再生能力、感染风险和慢性炎症的阻碍，而传统的移植物和惰性植入物往往存在宿主整合不良的问题。在此，我们报道了一种细胞外基质模拟水凝胶，通过动态硫醇-二硫化学和牺牲预血管化同步血管生成、成骨和免疫调节。用l-半胱氨酸乙酯功能化的透明质酸（HACys）与大蒜素交联，产生粘适应性、应力松弛的网络，其中I型胶原蛋白被挤压成加载vegf的线作为牺牲模板（HACys-VEGF@Coll-A）。酶降解后，这些线产生了vegf衬里的微通道，旨在引导血管快速进入。复合材料与骨髓间充质干细胞表现出良好的细胞相容性，选择性地增强了HUVEC的活力和扩散。此外，VEGF呈递构建体显著促进内皮管的形成和迁移，同时上调VEGF mRNA，证实保留了生物活性。在骨髓间充质干细胞中，含有vegf的水凝胶增加了碱性磷酸酶活性和矿物质沉积，并伴有成骨基因（ALP, COL1, RUNX2， OCN）的上调。值得注意的是，巨噬细胞从CD86high/CD206low表型向CD86low/CD206high表型转变，TNF-α降低，IL-10分泌增加，表明建立了促分辨免疫微环境。总的来说，HACys-VEGF@Coll-A形成了一个动态的、可重塑的支架，它整合了预制的血管导管，同时支持成骨和缓和炎症，从而解决了颅缺损修复的主要障碍，并保证了进一步的体内释放动力学、通道结构和力学评估。

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引用次数: 0

Cerium-cobalt composite nanoparticles (CC NPs) as a multifunctional reactive oxygen species (ROS) scavenger for psoriasis therapy 铈钴复合纳米颗粒（CC NPs）作为多功能活性氧（ROS）清除剂治疗银屑病

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-22 DOI: 10.1016/j.colsurfb.2026.115468

Jiao Zhang , Hao Sun , Hui Yuan , Wenyu Chen , Jialin Yan , Jie Ren , Ting Hu , Yuhang Li

Psoriasis is a chronic inflammatory disorder with a worldwide prevalence of 1–3 %, which has no cure. By inducing inflammation, keratinocyte hyperproliferation, and oxidative stress, reactive oxygen species (ROS) play a pivotal role in the pathogenesis of psoriasis. Although the clinical application of ROS-scavenging nanozymes is expected, their translation has been limited by complicated synthesis and high cost. To bypass these problems, in the present study, cerium cobalt composite nanoparticles (CC NPs) were fabricated via facile coprecipitation. The optimization results revealed that 8CC NPs (CeCo = 8:2) were optimal with uniform spherical morphology and enhanced oxygen vacancies, which enhanced the ROS-scavenging activities, and enabled the efficient scavenging of H₂O₂, O₂•^-, and •OH radicals. Then, 8CC NPs suppressed the intracellular ROS accumulation, inhibited proliferation, and attenuated the NF-κB inflammatory signaling in the IL-17-stimulated HaCaT keratinocytes. Additionally, in a murine imiquimod-induced psoriasis model, the treatment with 8CC NPs markedly alleviated the clinical symptoms, and no toxicity was observed. Therefore, CC NPs could serve as a novel, cost-effective, and biocompatible nanozyme platform with powerful ROS scavenging, simple synthesis, and promising inflammatory suppression, which provided strong support for their application in the translation of psoriasis. Given the inherent differences between murine models and human psoriasis, further studies are essential to validate the therapeutic potential of this nanozyme platform in clinical settings.

牛皮癣是一种慢性炎症性疾病，全球患病率为1-3 %，无法治愈。活性氧（reactive oxygen species， ROS）通过诱导炎症、角化细胞过度增生和氧化应激，在银屑病的发病机制中起关键作用。虽然清除活性氧纳米酶的临床应用前景广阔，但其合成复杂、成本高，限制了其翻译。为了解决这些问题，本研究采用易共沉淀法制备了铈钴复合纳米颗粒（CC NPs）。优化结果表明，8CC NPs （CeCo = 8:2）具有均匀的球形形貌和增强的氧空位，增强了活性氧清除能力，能够有效清除H2O2、O2•-和•OH自由基。然后，8CC NPs抑制细胞内ROS积累，抑制增殖，减弱il -17刺激的HaCaT角质形成细胞的NF-κB炎症信号。此外，在吡喹莫德诱导的小鼠银屑病模型中，8CC NPs治疗可显著缓解临床症状，且无毒性。因此，CC NPs具有清除ROS能力强、合成简单、抑制炎症等优点，可作为一种新型的、具有成本效益和生物相容性的纳米酶平台，为其在银屑病翻译中的应用提供了强有力的支持。考虑到小鼠模型和人类牛皮癣之间的内在差异，进一步的研究是必要的，以验证该纳米酶平台在临床环境中的治疗潜力。

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引用次数: 0

Recent advances in metal-organic framework-based nanozymes for cancer theranostics driven by synthetic innovation and machine learning design 由合成创新和机器学习设计驱动的用于癌症治疗的金属-有机框架纳米酶的最新进展

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-20 DOI: 10.1016/j.colsurfb.2026.115463

Sangeeta Yadav , Aditi Sarkar , Saurabh Shivalkar , Fiza Fatima , Siddharth Kumar Thakur , Ankita Chaudhary , Sintu Kumar Samanta , Amaresh Kumar Sahoo

Nanozymes are engineered nanomaterials designed at the atomic scale to fine-tune their structure, composition, and electronic properties, thereby creating active sites that mimic those of natural enzymes. Among these materials, Metal-organic frameworks (MOFs) are notable for their well-defined, porous frameworks, which are created by connecting metal ions or clusters with organic linkers. Their large surface area, adjustable porosity, and superior biocompatibility enable excellent catalytic activity. By containing specific catalytic functionalities, MOF-based nanozymes can mimic peroxidase, oxidase, catalase, and superoxide dismutase activities. These properties make them genuinely promising for biomedical applications, particularly in cancer diagnosis and therapy. Recent progress in synthetic design, post-synthetic modification, and machine learning–assisted optimization has enhanced their structural precision and catalytic efficiency. Furthermore, MOFs serve as multifunctional therapeutic platforms capable of supporting combined treatment strategies and producing synergistic therapeutic effects, thereby establishing their potential as next-generation systems for targeted cancer treatment and diagnostic integration.

纳米酶是在原子尺度上设计的工程纳米材料，可以微调其结构、组成和电子特性，从而产生模仿天然酶的活性位点。在这些材料中，金属有机框架（mof）以其定义良好的多孔框架而闻名，这些框架是通过将金属离子或簇与有机连接剂连接而形成的。它们的大表面积，可调节的孔隙率和优越的生物相容性使其具有优异的催化活性。通过含有特定的催化功能，mof基纳米酶可以模拟过氧化物酶、氧化酶、过氧化氢酶和超氧化物歧化酶的活性。这些特性使它们在生物医学应用，特别是癌症诊断和治疗方面具有真正的前景。合成设计、合成后修饰和机器学习辅助优化的最新进展提高了它们的结构精度和催化效率。此外，mof作为多功能治疗平台，能够支持联合治疗策略并产生协同治疗效果，从而确立其作为靶向癌症治疗和诊断整合的下一代系统的潜力。

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引用次数: 0

Naringenin nanosuspensions embedded glycyrrhizin-based hydrogel ameliorates cholestatic liver injury in mice by inhibiting oxidative stress and HMGB1-mediated inflammation 柚皮素纳米混悬液包埋甘草酸基水凝胶通过抑制氧化应激和hmgb1介导的炎症改善小鼠胆汁淤积性肝损伤

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-20 DOI: 10.1016/j.colsurfb.2026.115466

Qiqi Li , Yaochen Deng , Xiaolu Han , Yuhan Dong , Xianggen Wu , Wei Zhu , Meixing Yan

Naringenin (NAR) possesses remarkable hepatoprotective potential. However, its extremely low aqueous solubility and oral bioavailability greatly constrain its therapeutic efficacy. To overcome these limitations, we developed a novel oral nanodelivery system, NanoNAR@Glycygel, by embedding NAR nanosuspensions (NanoNAR) into a self-assembled glycyrrhizin-based hydrogel (Glycygel). The design of this delivery system improves solubility, enhances absorption, and provides synergistic hepatoprotective effects. NanoNAR, when stabilized by the natural biosurfactant glycyrrhizin, exhibited a uniform particle size of approximately 230 nm and showed markedly improved solubility in physiologically relevant media. The hydrogel network formed by Glycygel effectively encapsulated NanoNAR, further enhancing its solubility and controlled release behavior. Pharmacokinetic analyses revealed that NanoNAR@Glycygel significantly enhanced the oral bioavailability of NAR and increased its hepatic accumulation, demonstrating how the synergistic interplay between nanonization and the glycyrrhizin hydrogel matrix facilitates rapid absorption and sustained release. In a cholestatic liver injury mouse model, NanoNAR@Glycygel treatment markedly alleviated cholestasis and hepatic histopathological damage, restoring liver morphology and serum biochemical parameters to near-normal levels. Mechanistic investigations revealed for the first time that HMGB1 signaling is involved in this cholestatic liver injury, and NanoNAR@Glycygel exerted its potent therapeutic effect by inhibiting this signaling. The NanoNAR@Glycygel cloud also reduced malondialdehyde (MDA) levels and enhanced superoxide (SOD) activity, thereby mitigating oxidative injury. Collectively, these findings demonstrate that NanoNAR@Glycygel is a safe, simple, and highly effective oral delivery platform that not only unleashes the therapeutic potential of NAR but also highlights the distinctive advantages of glycyrrhizin-based matrices for the targeted oral delivery of hydrophobic natural bioactives.

柚皮素（Naringenin， NAR）具有显著的肝保护作用。然而，其极低的水溶性和口服生物利用度极大地限制了其治疗效果。为了克服这些限制，我们开发了一种新的口服纳米递送系统NanoNAR@Glycygel，通过将NAR纳米悬液（NanoNAR）嵌入自组装的甘草酸基水凝胶（Glycygel）中。这种给药系统的设计改善了溶解度，增强了吸收，并提供了协同的肝保护作用。经天然生物表面活性剂甘草酸稳定后，NanoNAR呈现出约230 nm的均匀粒径，并且在生理相关介质中的溶解度显著提高。Glycygel形成的水凝胶网络有效地包裹了NanoNAR，进一步增强了其溶解度和控释行为。药代动力学分析显示NanoNAR@Glycygel显著提高了NAR的口服生物利用度，并增加了其肝脏蓄积，表明纳米化与甘草酸水凝胶基质之间的协同相互作用促进了快速吸收和持续释放。在胆汁淤积性肝损伤小鼠模型中，NanoNAR@Glycygel治疗明显减轻了胆汁淤积和肝脏组织病理学损伤，使肝脏形态和血清生化参数恢复到接近正常水平。机制研究首次发现HMGB1信号通路参与了这种胆汁淤积性肝损伤，NanoNAR@Glycygel通过抑制该信号通路发挥其有效的治疗作用。NanoNAR@Glycygel云还降低了丙二醛（MDA）水平，增强了超氧化物（SOD）活性，从而减轻了氧化损伤。总之，这些发现表明NanoNAR@Glycygel是一种安全、简单、高效的口服给药平台，不仅释放了NAR的治疗潜力，而且突出了甘草酸基基质在靶向口服疏水天然生物活性物质方面的独特优势。

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