Colloids and Surfaces B: Biointerfaces最新文献_第4页

MoS2 nanoflowers surface decorated with CuS nanorods and carbon dots for fluorescent and ultrasound imaging in cancer therapy 用cu纳米棒和碳点修饰的二硫化钼纳米花表面用于肿瘤的荧光和超声成像。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-07 DOI: 10.1016/j.colsurfb.2025.114503

Nishakavya Saravanan , Anandhakumar Sundaramurthy , Sukho Park

In recent years, the design of various ultrasound responsive echogenic nanomaterials offers many advantages such as deep tissue penetration, high signal intensity, colloidal stability, biocompatibility and less expensive for ultrasound-based cancer cell imaging while providing the option to monitor the progress of tumor volume during the treatment. Further, the ability of nanomaterials to combine photo-thermal therapy (PTT) and chemotherapy has opened a new avenue in the development of cancer theranostics for synergistic cancer therapy. Herein, we report MoS₂ nanoflowers (NFs) surface decorated with CuS nanorods (NRs) and folic acid-derived carbon dots (FACDs) using cystine-polyethyleneimine (PEI) linker for PTT-chemotherapy. The size of NFs was found to be 350 ± 50 nm which increased to 500 ± 50 nm after surface decoration. The morphology of MoS₂ NFs before and after surface decoration was investigated using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and UV-Vis-NIR spectroscopy. The photo-thermal heat generation was found to be increasing as a function of the concentration of NFs. The encapsulation efficiency of doxorubicin (DOX) and photo-thermal conversion efficiency (PCE) for surface-decorated MoS₂ NFs (MoS₂@CuS/FACDs NFs) was estimated to be 42 and 44 %, respectively. The surface decoration of CuS NRs and FACDs on MoS₂ NFs not only improved the anticancer activity but also increased the signal intensity in ultrasound and fluorescence imaging of cancer cells. The MoS₂@CuS/FACDs NFs exhibited excellent cytotoxicity against MDA-MB-231 cancer cells. Hence, the hybrid system demonstrated here showed high potential for use as a combined probe for non-invasive ultrasound imaging and fluorescence imaging for PTT-chemotherapy.

近年来，各种超声响应回声纳米材料的设计为超声癌细胞成像提供了许多优点，如组织深度穿透，高信号强度，胶体稳定性，生物相容性和更便宜，同时提供了在治疗过程中监测肿瘤体积进展的选择。此外，纳米材料结合光热疗法（PTT）和化疗的能力为癌症治疗学的发展开辟了一条新的途径，用于协同癌症治疗。在此，我们报道了使用半胱氨酸-聚乙烯亚胺（PEI）连接剂修饰CuS纳米棒（nr）和叶酸衍生碳点（FACDs）的MoS2纳米花（NFs）表面用于ptt化疗。纳米颗粒的粒径为350 ± 50 nm，经表面修饰后增大到500 ± 50 nm。采用扫描电镜、x射线衍射、x射线光电子能谱、傅里叶变换红外光谱和紫外-可见-近红外光谱研究表面修饰前后MoS2 NFs的形貌。光热产热随着纳米颗粒浓度的增加而增加。阿霉素（DOX）的包封效率和表面修饰的MoS2 NFs （MoS2@CuS/FACDs NFs）的光热转换效率（PCE）估计分别为42%和44% %。在MoS2 NFs表面修饰CuS NRs和FACDs，不仅提高了抗癌活性，而且增加了癌细胞超声和荧光成像的信号强度。MoS2@CuS/FACDs NFs对MDA-MB-231癌细胞表现出优异的细胞毒性。因此，这里展示的混合系统显示了作为ptt化疗的无创超声成像和荧光成像联合探针的巨大潜力。

{"title":"MoS2 nanoflowers surface decorated with CuS nanorods and carbon dots for fluorescent and ultrasound imaging in cancer therapy","authors":"Nishakavya Saravanan , Anandhakumar Sundaramurthy , Sukho Park","doi":"10.1016/j.colsurfb.2025.114503","DOIUrl":"10.1016/j.colsurfb.2025.114503","url":null,"abstract":"<div><div>In recent years, the design of various ultrasound responsive echogenic nanomaterials offers many advantages such as deep tissue penetration, high signal intensity, colloidal stability, biocompatibility and less expensive for ultrasound-based cancer cell imaging while providing the option to monitor the progress of tumor volume during the treatment. Further, the ability of nanomaterials to combine photo-thermal therapy (PTT) and chemotherapy has opened a new avenue in the development of cancer theranostics for synergistic cancer therapy. Herein, we report MoS<sub>2</sub> nanoflowers (NFs) surface decorated with CuS nanorods (NRs) and folic acid-derived carbon dots (FACDs) using cystine-polyethyleneimine (PEI) linker for PTT-chemotherapy. The size of NFs was found to be 350 ± 50 nm which increased to 500 ± 50 nm after surface decoration. The morphology of MoS<sub>2</sub> NFs before and after surface decoration was investigated using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and UV-Vis-NIR spectroscopy. The photo-thermal heat generation was found to be increasing as a function of the concentration of NFs. The encapsulation efficiency of doxorubicin (DOX) and photo-thermal conversion efficiency (PCE) for surface-decorated MoS<sub>2</sub> NFs (MoS<sub>2</sub>@CuS/FACDs NFs) was estimated to be 42 and 44 %, respectively. The surface decoration of CuS NRs and FACDs on MoS<sub>2</sub> NFs not only improved the anticancer activity but also increased the signal intensity in ultrasound and fluorescence imaging of cancer cells. The MoS<sub>2</sub>@CuS/FACDs NFs exhibited excellent cytotoxicity against MDA-MB-231 cancer cells. Hence, the hybrid system demonstrated here showed high potential for use as a combined probe for non-invasive ultrasound imaging and fluorescence imaging for PTT-chemotherapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114503"},"PeriodicalIF":5.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The latest applications of exosome-mediated drug delivery in anticancer therapies 外泌体介导给药在抗癌治疗中的最新应用。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-06 DOI: 10.1016/j.colsurfb.2025.114500

Zhiwei Wen , Wei Zhang , Wei Wu

In recent years, the significant role of anticancer drugs in cancer treatment has garnered considerable attention. However, the application of these drugs is largely limited by their short half-life in blood circulation, low cellular uptake efficiency, and off-target effects. Exosomes, which serve as crucial messengers in intercellular communication, exhibit unique advantages in molecular delivery compared to traditional synthetic carriers, thereby offering new possibilities for modern drug delivery systems. Exosomes possess organotropic functions and are naturally produced by cells, making them promising candidates for natural drug delivery systems with organotropic properties and minimal side effects. These naturally derived carriers can achieve stable, efficient, and selective delivery of anticancer drugs, thereby enhancing the efficacy and potential of anticancer agents in cancer immunotherapy. This review provides a concise overview of the unique characteristics of exosomes related to anticancer drug delivery, strategies for utilizing exosomes as carriers in cancer therapy, and the latest advancements in the field.

近年来，抗癌药物在癌症治疗中的重要作用引起了人们的广泛关注。然而，这些药物在血液循环中的半衰期短、细胞摄取效率低以及脱靶效应在很大程度上限制了它们的应用。外泌体作为细胞间通讯的重要信使，与传统的合成载体相比，在分子传递方面表现出独特的优势，从而为现代药物传递系统提供了新的可能性。外泌体具有器官亲和性功能，由细胞自然产生，使其成为具有器官亲和性和最小副作用的天然药物输送系统的有希望的候选者。这些天然衍生载体可以实现抗癌药物稳定、高效和选择性的递送，从而提高抗癌药物在癌症免疫治疗中的功效和潜力。本文综述了外泌体与抗癌药物传递相关的独特特性、利用外泌体作为癌症治疗载体的策略以及该领域的最新进展。

{"title":"The latest applications of exosome-mediated drug delivery in anticancer therapies","authors":"Zhiwei Wen , Wei Zhang , Wei Wu","doi":"10.1016/j.colsurfb.2025.114500","DOIUrl":"10.1016/j.colsurfb.2025.114500","url":null,"abstract":"<div><div>In recent years, the significant role of anticancer drugs in cancer treatment has garnered considerable attention. However, the application of these drugs is largely limited by their short half-life in blood circulation, low cellular uptake efficiency, and off-target effects. Exosomes, which serve as crucial messengers in intercellular communication, exhibit unique advantages in molecular delivery compared to traditional synthetic carriers, thereby offering new possibilities for modern drug delivery systems. Exosomes possess organotropic functions and are naturally produced by cells, making them promising candidates for natural drug delivery systems with organotropic properties and minimal side effects. These naturally derived carriers can achieve stable, efficient, and selective delivery of anticancer drugs, thereby enhancing the efficacy and potential of anticancer agents in cancer immunotherapy. This review provides a concise overview of the unique characteristics of exosomes related to anticancer drug delivery, strategies for utilizing exosomes as carriers in cancer therapy, and the latest advancements in the field.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114500"},"PeriodicalIF":5.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on surface modification and coating technologies of biomedical NiTi alloys 医用NiTi合金表面改性及涂层技术研究进展。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-06 DOI: 10.1016/j.colsurfb.2025.114496

Xiang Li , Ying Yang , Hui Shen , Meng Zhou , Bingmin Huang , Lishan Cui , Shijie Hao

NiTi alloys are an important class of biomaterials with extensive clinical applications such as cardiovascular stents, orthodontic arch-wires, esophageal stents, orthopedic implants and more. However, the long-term implantation of NiTi alloys presents significant challenges due to their susceptibility to wear, corrosion and the excessive release of harmful nickel ions. These factors can severely compromise both the biocompatibility and the overall service life of the implants. To better meet the demands for safety, durability and superior biological performance after implantation, surface modification of NiTi alloys has become a focal point of current research. Based on the fundamental properties of the NiTi alloys and the challenges encountered in their practical applications, this article provides a focused review of recent advances in improving their corrosion resistance, wear resistance, antibacterial properties and biological performance through surface modification and coating techniques. In addition, the paper outlines current research challenges and proposes recommendations for future development directions.

镍钛合金是一类重要的生物材料，具有广泛的临床应用，如心血管支架、正畸弓丝、食管支架、骨科植入物等。然而，NiTi合金易磨损、易腐蚀，有害镍离子释放过多，长期注入存在很大的挑战。这些因素会严重影响种植体的生物相容性和整体使用寿命。为了更好地满足植入后对安全性、耐久性和优异生物性能的要求，对NiTi合金进行表面改性已成为当前研究的热点。本文从NiTi合金的基本特性和在实际应用中遇到的挑战出发，重点综述了近年来通过表面改性和涂层技术提高其耐腐蚀、耐磨性、抗菌和生物性能的研究进展。此外，本文还概述了当前研究面临的挑战，并对未来的发展方向提出了建议。

{"title":"Research progress on surface modification and coating technologies of biomedical NiTi alloys","authors":"Xiang Li , Ying Yang , Hui Shen , Meng Zhou , Bingmin Huang , Lishan Cui , Shijie Hao","doi":"10.1016/j.colsurfb.2025.114496","DOIUrl":"10.1016/j.colsurfb.2025.114496","url":null,"abstract":"<div><div>NiTi alloys are an important class of biomaterials with extensive clinical applications such as cardiovascular stents, orthodontic arch-wires, esophageal stents, orthopedic implants and more. However, the long-term implantation of NiTi alloys presents significant challenges due to their susceptibility to wear, corrosion and the excessive release of harmful nickel ions. These factors can severely compromise both the biocompatibility and the overall service life of the implants. To better meet the demands for safety, durability and superior biological performance after implantation, surface modification of NiTi alloys has become a focal point of current research. Based on the fundamental properties of the NiTi alloys and the challenges encountered in their practical applications, this article provides a focused review of recent advances in improving their corrosion resistance, wear resistance, antibacterial properties and biological performance through surface modification and coating techniques. In addition, the paper outlines current research challenges and proposes recommendations for future development directions.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114496"},"PeriodicalIF":5.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in nanotherapy-based treatment of epilepsy 纳米治疗癫痫的最新进展。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-06 DOI: 10.1016/j.colsurfb.2025.114499

Peng Chen , Shudong Wang , Heming Zhang , Jian Li

Epilepsy is a complex neurological disorder characterized by recurrent seizures affecting millions of people worldwide. Despite advances in drug therapy, a significant proportion of patients remain resistant to conventional antiepileptic drugs (AEDs) due to challenges such as impermeability of the blood-brain barrier (BBB), multidrug resistance, and multifaceted epileptogenesis. Nanotechnology offers promising strategies to overcome these barriers by enhancing drug delivery across the BBB, improving target specificity and minimizing systemic side effects. This review explores recent advances in different innovative strategies of nanodelivery systems for epilepsy therapy, and we will discuss the design principles, mechanisms of action and therapeutic efficacy of these nanodelivery systems. In addition, we discuss the challenges and limitations that hinder the clinical translation of nanomedicine-based therapies for epilepsy. We emphasize the need for personalized and multidisciplinary approaches as well as the importance of continued research and interdisciplinary collaboration in order to translate these innovative strategies into effective therapies. Ultimately, the use of nanotechnology has the potential to enhance seizure control, reduce the burden of epilepsy, and improve the quality of life of patients affected by this complex neurological disorder. Nanotechnology-based drug delivery systems may usher in a new era of precision medicine for epilepsy treatment.

癫痫是一种复杂的神经系统疾病，其特征是反复发作，影响全世界数百万人。尽管药物治疗取得了进展，但由于血脑屏障（BBB）的不渗透性、多药耐药和多方面的癫痫发生等挑战，很大一部分患者仍然对传统抗癫痫药物（aed）产生耐药性。纳米技术为克服这些障碍提供了有希望的策略，通过增强药物在血脑屏障上的传递，提高靶特异性和最小化全身副作用。本文综述了用于癫痫治疗的纳米递送系统的不同创新策略的最新进展，并将讨论这些纳米递送系统的设计原则、作用机制和治疗效果。此外，我们还讨论了阻碍纳米药物治疗癫痫临床转化的挑战和限制。我们强调个性化和多学科方法的必要性，以及持续研究和跨学科合作的重要性，以便将这些创新策略转化为有效的治疗方法。最终，纳米技术的使用有可能加强癫痫发作的控制，减轻癫痫的负担，并改善受这种复杂神经系统疾病影响的患者的生活质量。以纳米技术为基础的药物输送系统可能迎来癫痫治疗精准医学的新时代。

{"title":"Recent advances in nanotherapy-based treatment of epilepsy","authors":"Peng Chen , Shudong Wang , Heming Zhang , Jian Li","doi":"10.1016/j.colsurfb.2025.114499","DOIUrl":"10.1016/j.colsurfb.2025.114499","url":null,"abstract":"<div><div>Epilepsy is a complex neurological disorder characterized by recurrent seizures affecting millions of people worldwide. Despite advances in drug therapy, a significant proportion of patients remain resistant to conventional antiepileptic drugs (AEDs) due to challenges such as impermeability of the blood-brain barrier (BBB), multidrug resistance, and multifaceted epileptogenesis. Nanotechnology offers promising strategies to overcome these barriers by enhancing drug delivery across the BBB, improving target specificity and minimizing systemic side effects. This review explores recent advances in different innovative strategies of nanodelivery systems for epilepsy therapy, and we will discuss the design principles, mechanisms of action and therapeutic efficacy of these nanodelivery systems. In addition, we discuss the challenges and limitations that hinder the clinical translation of nanomedicine-based therapies for epilepsy. We emphasize the need for personalized and multidisciplinary approaches as well as the importance of continued research and interdisciplinary collaboration in order to translate these innovative strategies into effective therapies. Ultimately, the use of nanotechnology has the potential to enhance seizure control, reduce the burden of epilepsy, and improve the quality of life of patients affected by this complex neurological disorder. Nanotechnology-based drug delivery systems may usher in a new era of precision medicine for epilepsy treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114499"},"PeriodicalIF":5.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational exploration of the self-aggregation mechanisms of phenol-soluble modulins β1 and β2 in Staphylococcus aureus biofilms 金黄色葡萄球菌生物膜中酚溶性调节素β1和β2自聚集机制的计算探索。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-05 DOI: 10.1016/j.colsurfb.2025.114498

Huan Xu , Xiaohan Zhang , Zhongyue Lv , Fengjuan Huang , Yu Zou , Chuang Wang , Feng Ding , Yunxiang Sun

The formation of functional bacterial amyloids by phenol-soluble modulins (PSMs) in Staphylococcus aureus is a critical component of biofilm-associated infections, providing robust protective barriers against antimicrobial agents and immune defenses. Clarifying the molecular mechanisms of PSM self-assembly within the biofilm matrix is essential for developing strategies to disrupt biofilm integrity and combat biofilm-related infections. In this study, we analyzed the self-assembly dynamics of PSM-β1 and PSM-β2 by examining their folding and dimerization through long-timescale atomistic discrete molecular dynamics simulations. Our findings revealed that both peptides primarily adopt helical structures as monomers but shift to β-sheets upon dimerization. Monomeric state, PSM-β1 exhibited frequent transitions between helical and β-sheet forms, while PSM-β2 largely retained a helical structure. Upon dimerization, both peptides showed pronounced β-sheet formation around conserved C-terminal residues 21–44. Residues 21–33, largely unstructured as monomers, demonstrated strong tendencies for β-sheet formation and intermolecular interactions, underscoring their central role in the self-assembly of both peptides. Additionally, the PSM-β1 N-terminus formed β-sheets only when interacting with the C-terminus, whereas the PSM-β2 N-terminus remained helical and uninvolved in β-sheet formation. These distinct aggregation behaviors likely contribute to biofilm dynamics, with C-terminal regions facilitating biofilm formation and N-terminal regions influencing stability. Targeting residues 21–33 in PSM-β1 and PSM-β2 offers a promising therapeutic approach for disrupting biofilm integrity. This study advances our understanding of PSM-β1 and PSM-β2 self-assembly and presents new targets for drug design against biofilm-associated diseases.

金黄色葡萄球菌中酚溶性调节素（psm）形成功能性细菌淀粉样蛋白是生物膜相关感染的关键组成部分，为抗微生物药物和免疫防御提供了强大的保护屏障。阐明PSM在生物膜基质内自组装的分子机制对于制定破坏生物膜完整性和对抗生物膜相关感染的策略至关重要。在这项研究中，我们通过长时间尺度的原子离散分子动力学模拟，研究了PSM-β1和PSM-β2的折叠和二聚化，分析了它们的自组装动力学。我们的研究结果表明，这两种肽主要采用螺旋结构作为单体，但在二聚化时转变为β-片。在单体状态下，PSM-β1表现出螺旋和β片之间的频繁转变，而PSM-β2则基本保持螺旋结构。二聚化后，两种多肽在保守的c端残基21-44周围显示明显的β-薄片形成。残基21-33大部分是非结构化单体，表现出β-薄片形成和分子间相互作用的强烈倾向，强调了它们在两种肽的自组装中的核心作用。此外，PSM-β1 n -端仅在与c -端相互作用时形成β-片，而PSM-β2 n -端则保持螺旋状，不参与β-片的形成。这些不同的聚集行为可能有助于生物膜动力学，c端区域促进生物膜的形成，n端区域影响稳定性。靶向PSM-β1和PSM-β2残基21-33为破坏生物膜完整性提供了一种有前景的治疗方法。该研究促进了我们对PSM-β1和PSM-β2自组装的认识，并为生物膜相关疾病的药物设计提供了新的靶点。

{"title":"Computational exploration of the self-aggregation mechanisms of phenol-soluble modulins β1 and β2 in Staphylococcus aureus biofilms","authors":"Huan Xu , Xiaohan Zhang , Zhongyue Lv , Fengjuan Huang , Yu Zou , Chuang Wang , Feng Ding , Yunxiang Sun","doi":"10.1016/j.colsurfb.2025.114498","DOIUrl":"10.1016/j.colsurfb.2025.114498","url":null,"abstract":"<div><div>The formation of functional bacterial amyloids by phenol-soluble modulins (PSMs) in <em>Staphylococcus aureus</em> is a critical component of biofilm-associated infections, providing robust protective barriers against antimicrobial agents and immune defenses. Clarifying the molecular mechanisms of PSM self-assembly within the biofilm matrix is essential for developing strategies to disrupt biofilm integrity and combat biofilm-related infections. In this study, we analyzed the self-assembly dynamics of PSM-β1 and PSM-β2 by examining their folding and dimerization through long-timescale atomistic discrete molecular dynamics simulations. Our findings revealed that both peptides primarily adopt helical structures as monomers but shift to β-sheets upon dimerization. Monomeric state, PSM-β1 exhibited frequent transitions between helical and β-sheet forms, while PSM-β2 largely retained a helical structure. Upon dimerization, both peptides showed pronounced β-sheet formation around conserved C-terminal residues 21–44. Residues 21–33, largely unstructured as monomers, demonstrated strong tendencies for β-sheet formation and intermolecular interactions, underscoring their central role in the self-assembly of both peptides. Additionally, the PSM-β1 N-terminus formed β-sheets only when interacting with the C-terminus, whereas the PSM-β2 N-terminus remained helical and uninvolved in β-sheet formation. These distinct aggregation behaviors likely contribute to biofilm dynamics, with C-terminal regions facilitating biofilm formation and N-terminal regions influencing stability. Targeting residues 21–33 in PSM-β1 and PSM-β2 offers a promising therapeutic approach for disrupting biofilm integrity. This study advances our understanding of PSM-β1 and PSM-β2 self-assembly and presents new targets for drug design against biofilm-associated diseases.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"Article 114498"},"PeriodicalIF":5.4,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coaxial bioprinting of a three-layer vascular structure exhibiting blood-brain barrier function for neuroprotective drug screening 具有血脑屏障功能的三层血管结构的同轴生物打印用于神经保护药物筛选。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-03 DOI: 10.1016/j.colsurfb.2025.114494

Zhichao Wang , Chuanzhen Huang , Zhenyu Shi , Hanlian Liu , Xu Han , Zhuang Chen , Shuying Li , Zhen Wang , Jun Huang

The in vitro blood-brain barrier (BBB) structures can offer advantages for studying cerebrovascular functions and developing neuroprotective drugs. However, currently developed BBB models are overly simplistic and inadequate for replicating the complex three-dimensional architecture of the in vivo BBB. In this study, a method is introduced for fabricating a three-layer vascular structure exhibiting BBB function using a coaxial extrusion bioprinting technique with a two-layer nozzle. Photocurable materials were incorporated into the inner layer of the coaxial nozzle, and photoinitiators from the outer layer diffused into the inner layer. As a result, only the materials in the inner layer at the interface between the inner and outer layers underwent crosslinking upon UV exposure. After removing the uncrosslinked materials, a two-layer vascular structure can be formed. Subsequently, a three-layer structure was established after seeding endothelial cells. The perfusion experiments demonstrated that the vascular structure facilitated the continuous flow of culture medium, thereby providing nutrients and oxygen to the surrounding neural tissue. The drug screening analysis indicated that this vascular structure could possess barrier function, allowing the passage of small molecular drugs while effectively blocking macromolecular drugs. Overall, these results suggest that the three-layer vascular structure exhibits excellent perfusion capacity and barrier function, making it a promising candidate for neuroprotective drug screening.

体外血脑屏障（BBB）结构为研究脑血管功能和开发神经保护药物提供了有利条件。然而，目前开发的血脑屏障模型过于简单，不足以复制体内血脑屏障复杂的三维结构。在这项研究中，介绍了一种利用同轴挤压生物打印技术和两层喷嘴制造具有血脑屏障功能的三层血管结构的方法。将光固化材料掺入同轴喷嘴的内层，光引发剂从外层扩散到内层。因此，在紫外线照射下，只有内层和外层之间的界面处的材料发生交联。去除非交联材料后，可形成两层维管结构。随后，内皮细胞植入后形成三层结构。灌注实验表明，血管结构促进了培养基的连续流动，从而为周围神经组织提供营养和氧气。药物筛选分析表明，该血管结构具有屏障功能，允许小分子药物通过，同时有效阻断大分子药物。综上所述，三层血管结构具有良好的灌注能力和屏障功能，是神经保护药物筛选的理想候选者。

{"title":"Coaxial bioprinting of a three-layer vascular structure exhibiting blood-brain barrier function for neuroprotective drug screening","authors":"Zhichao Wang , Chuanzhen Huang , Zhenyu Shi , Hanlian Liu , Xu Han , Zhuang Chen , Shuying Li , Zhen Wang , Jun Huang","doi":"10.1016/j.colsurfb.2025.114494","DOIUrl":"10.1016/j.colsurfb.2025.114494","url":null,"abstract":"<div><div>The in vitro blood-brain barrier (BBB) structures can offer advantages for studying cerebrovascular functions and developing neuroprotective drugs. However, currently developed BBB models are overly simplistic and inadequate for replicating the complex three-dimensional architecture of the in vivo BBB. In this study, a method is introduced for fabricating a three-layer vascular structure exhibiting BBB function using a coaxial extrusion bioprinting technique with a two-layer nozzle. Photocurable materials were incorporated into the inner layer of the coaxial nozzle, and photoinitiators from the outer layer diffused into the inner layer. As a result, only the materials in the inner layer at the interface between the inner and outer layers underwent crosslinking upon UV exposure. After removing the uncrosslinked materials, a two-layer vascular structure can be formed. Subsequently, a three-layer structure was established after seeding endothelial cells. The perfusion experiments demonstrated that the vascular structure facilitated the continuous flow of culture medium, thereby providing nutrients and oxygen to the surrounding neural tissue. The drug screening analysis indicated that this vascular structure could possess barrier function, allowing the passage of small molecular drugs while effectively blocking macromolecular drugs. Overall, these results suggest that the three-layer vascular structure exhibits excellent perfusion capacity and barrier function, making it a promising candidate for neuroprotective drug screening.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114494"},"PeriodicalIF":5.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PAMAM/miR-144 nanocarrier system inhibits the migration of gastric cancer by targeting mTOR signal transduction pathway PAMAM/miR-144纳米载体系统通过靶向mTOR信号转导通路抑制胃癌的迁移。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-03 DOI: 10.1016/j.colsurfb.2024.114492

Yayun Qian , Dongxu Zhu , Qiong Xu , Yujie Wang , Xiwen Chen , Weiwei Hua , Juqun Xi , Feng Lu

Exogenous microRNA-144 (miR-144) is considered as a potential biological drug for gastric cancer because of its biological activity to inhibit the epithelial-mesenchymal transition (EMT). However, the specific molecular mechanisms have not been fully revealed. In addition, their vulnerability to degradation by RNA enzymes in the blood limits their bioavailability. In this paper, a polyamidoamine (PAMAM)-wrapped miR-144 (PAMAM/miR-144) is prepared as a nanocarrier system to protect miR-144 from nuclease degradation. The PAMAM/miR-144 nanocarrier system achieves the optimal antitumor activity against gastric cancer migration and reduce mTOR protein expression by transferring miR-144 into human gastric cancer HGC-27 cells. At the same time, the PAMAM/miR-144 nanocarrier system significantly decreases the EMT via targeting mTOR signal pathway in HGC-27 cells and noticeably inhibited the growth of subcutaneous gastric cancer xenografts in nude mice. PAMAM/miR-144 nanocarrier system has effectively improved the bioavailability of miR-144, thus providing a promising combination modality for anticancer therapy.

外源性microRNA-144 （miR-144）具有抑制上皮-间质转化（epithelial-mesenchymal transition， EMT）的生物活性，被认为是治疗胃癌的潜在生物药物。然而，具体的分子机制尚未完全揭示。此外，它们易被血液中的RNA酶降解，限制了它们的生物利用度。本文制备了聚酰胺胺（PAMAM）包裹的miR-144 （PAMAM/miR-144）作为纳米载体体系来保护miR-144免受核酸酶降解。PAMAM/miR-144纳米载体系统通过将miR-144转移到人胃癌HGC-27细胞中，实现对胃癌迁移的最佳抗肿瘤活性，并降低mTOR蛋白的表达。同时，PAMAM/miR-144纳米载体系统通过靶向mTOR信号通路，显著降低HGC-27细胞的EMT，显著抑制裸鼠皮下胃癌异种移植物的生长。PAMAM/miR-144纳米载体体系有效提高了miR-144的生物利用度，为抗癌治疗提供了一种很有前景的联合方式。

{"title":"PAMAM/miR-144 nanocarrier system inhibits the migration of gastric cancer by targeting mTOR signal transduction pathway","authors":"Yayun Qian , Dongxu Zhu , Qiong Xu , Yujie Wang , Xiwen Chen , Weiwei Hua , Juqun Xi , Feng Lu","doi":"10.1016/j.colsurfb.2024.114492","DOIUrl":"10.1016/j.colsurfb.2024.114492","url":null,"abstract":"<div><div>Exogenous microRNA-144 (miR-144) is considered as a potential biological drug for gastric cancer because of its biological activity to inhibit the epithelial-mesenchymal transition (EMT). However, the specific molecular mechanisms have not been fully revealed. In addition, their vulnerability to degradation by RNA enzymes in the blood limits their bioavailability. In this paper, a polyamidoamine (PAMAM)-wrapped miR-144 (PAMAM/miR-144) is prepared as a nanocarrier system to protect miR-144 from nuclease degradation. The PAMAM/miR-144 nanocarrier system achieves the optimal antitumor activity against gastric cancer migration and reduce mTOR protein expression by transferring miR-144 into human gastric cancer HGC-27 cells. At the same time, the PAMAM/miR-144 nanocarrier system significantly decreases the EMT via targeting mTOR signal pathway in HGC-27 cells and noticeably inhibited the growth of subcutaneous gastric cancer xenografts in nude mice. PAMAM/miR-144 nanocarrier system has effectively improved the bioavailability of miR-144, thus providing a promising combination modality for anticancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114492"},"PeriodicalIF":5.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of EMAP-II-targeted anti-angiogenesis and photodynamic therapy using zinc phthalocyanine nanosystem for enhanced cancer treatment 结合emap - ii靶向抗血管生成和光动力治疗，使用锌酞菁纳米系统增强癌症治疗。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-03 DOI: 10.1016/j.colsurfb.2024.114493

Liyun Chen , Linlin Li , Hailong Zhao , Hao Li , Jiahui Li , Chao Li , Yang Zhou , Luxuan Yang , Jun Liang , Honglian Zhang , Juan Li , Peng Xu , Cai Yuan , Zhenhua Liu , Mingdong Huang , Longguang Jiang

Angiogenesis provides essential nutrients and oxygen to tumors during tumorigenesis, facilitating invasion and metastasis. Consequently, inhibiting tumor angiogenesis is an established strategy in anti-cancer therapy. In this study, we engineered a dual-function nanosystem with both antiangiogenic and photodynamic properties. We transformed the hydrophobic photosensitizer zinc phthalocyanine (PS) into a hydrophilic form via protein renaturation, resulting in a novel photosensitizer: Monocyte-Activating Polypeptide-II (EMAP-II:PS@NPs). Characterization through dynamic light scattering (DLS) and UV–vis spectroscopy showed that these nanoparticles exhibited uniform size and stability, and enhanced solubility. We further demonstrated that EMAP-II:PS@NPs effectively target tumor vascular endothelia causing intracellular photodynamic cytotoxicity. Notably, EMAP-II:PS@NPs achieved effective ablation of solid tumors at significantly reduced dosages of drugs compared to conventional therapies, due to their potent apoptotic effects on light-exposed cells. This study highlights the potential of combining anti-angiogenic activity with phototherapy, paving the way for innovative cancer treatment strategies.

血管生成在肿瘤发生过程中为肿瘤提供必需的营养和氧气，促进肿瘤的侵袭和转移。因此，抑制肿瘤血管生成是抗癌治疗的既定策略。在这项研究中，我们设计了一种具有抗血管生成和光动力特性的双功能纳米系统。我们将疏水性光敏剂酞菁锌（PS）通过蛋白质复性转化为亲水性形式，得到了一种新型光敏剂：单核细胞激活多肽ii （EMAP-II:PS@NPs）。动态光散射（DLS）和紫外可见光谱表征表明，这些纳米颗粒具有均匀的尺寸和稳定性，并且具有增强的溶解度。我们进一步证明EMAP-II:PS@NPs有效靶向肿瘤血管内皮，引起细胞内光动力细胞毒性。值得注意的是，与传统疗法相比，EMAP-II:PS@NPs在显著减少的药物剂量下实现了实体瘤的有效消融，这是由于它们对暴露于光下的细胞具有强大的凋亡作用。这项研究强调了将抗血管生成活性与光疗相结合的潜力，为创新的癌症治疗策略铺平了道路。

{"title":"Integration of EMAP-II-targeted anti-angiogenesis and photodynamic therapy using zinc phthalocyanine nanosystem for enhanced cancer treatment","authors":"Liyun Chen , Linlin Li , Hailong Zhao , Hao Li , Jiahui Li , Chao Li , Yang Zhou , Luxuan Yang , Jun Liang , Honglian Zhang , Juan Li , Peng Xu , Cai Yuan , Zhenhua Liu , Mingdong Huang , Longguang Jiang","doi":"10.1016/j.colsurfb.2024.114493","DOIUrl":"10.1016/j.colsurfb.2024.114493","url":null,"abstract":"<div><div>Angiogenesis provides essential nutrients and oxygen to tumors during tumorigenesis, facilitating invasion and metastasis. Consequently, inhibiting tumor angiogenesis is an established strategy in anti-cancer therapy. In this study, we engineered a dual-function nanosystem with both antiangiogenic and photodynamic properties. We transformed the hydrophobic photosensitizer zinc phthalocyanine (PS) into a hydrophilic form via protein renaturation, resulting in a novel photosensitizer: Monocyte-Activating Polypeptide-II (EMAP-II:PS@NPs). Characterization through dynamic light scattering (DLS) and UV–vis spectroscopy showed that these nanoparticles exhibited uniform size and stability, and enhanced solubility. We further demonstrated that EMAP-II:PS@NPs effectively target tumor vascular endothelia causing intracellular photodynamic cytotoxicity. Notably, EMAP-II:PS@NPs achieved effective ablation of solid tumors at significantly reduced dosages of drugs compared to conventional therapies, due to their potent apoptotic effects on light-exposed cells. This study highlights the potential of combining anti-angiogenic activity with phototherapy, paving the way for innovative cancer treatment strategies.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"Article 114493"},"PeriodicalIF":5.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-assembled water soluble and bone-targeting phosphorylated quercetin ameliorates postmenopausal osteoporosis in ovariectomy mice 自组装水溶性和骨靶向磷酸化槲皮素改善卵巢切除术小鼠绝经后骨质疏松症。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-03 DOI: 10.1016/j.colsurfb.2025.114495

Peng Luo , Yanlong Zhong , Xiaowei Yang , Qi Lai , Shaorong Huang , Xiaoyong Zhang , Bin Zhang , Yen Wei

Natural compounds have shown promising application prospects in preventing or treating various diseases, including osteoporosis on account of their abundant sources, low price, multi-targeting and multiple biological effects. As a bioactive natural product, quercetin (Que) has previously demonstrated to ameliorate osteoporosis (OP), however, its poor bioavailability resulting from low water solubility, poor stability and lack of bone-targeting largely restricted its efficacy and clinical applications. Inspired by the bone-targeting capability of phosphate compounds, we reported a one-step procedure for synthesis of phosphorylated Que (p-Que) by direct phosphorylating phenol groups of Que for the first time. The phosphate groups on p-Que could not only improve the water dispersibility of Que, but also endow p-Que desirable bioavailability and bone-targeting feature. The results from biological assays suggested that p-Que could inhibit osteoclastogenesis and bone resorption and alleviate trabeculae loss in osteoporotic mice. In conclusion, this work demonstrated that phosphorylation strategy can effectively solve low water solubility, lack of bone-targeting capability and poor bioavailability of natural compounds, providing a novel and efficient approach for development of OP nanomedicines.

天然化合物以其来源丰富、价格低廉、多靶点和多种生物效应在预防或治疗包括骨质疏松症在内的多种疾病方面显示出广阔的应用前景。槲皮素（quercetin, Que）作为一种具有生物活性的天然产物，曾被证明具有改善骨质疏松症（osteoporosis， OP）的作用，但由于其水溶性低、稳定性差、缺乏骨靶向性等原因，其生物利用度较差，在很大程度上限制了其疗效和临床应用。受磷酸盐化合物骨靶向能力的启发，我们首次报道了通过直接磷酸化Que的酚基一步合成磷酸化Que （p-Que）的方法。p-Que上的磷酸基团不仅提高了Que的水分散性，而且赋予了p-Que良好的生物利用度和骨靶向特性。生物实验结果表明，p-Que能抑制骨质疏松小鼠破骨细胞生成和骨吸收，减轻骨质疏松小鼠小梁丢失。综上所述，本研究表明磷酸化策略可以有效解决天然化合物水溶性低、骨靶向能力不足和生物利用度差的问题，为OP纳米药物的开发提供了一种新的高效途径。

{"title":"Self-assembled water soluble and bone-targeting phosphorylated quercetin ameliorates postmenopausal osteoporosis in ovariectomy mice","authors":"Peng Luo , Yanlong Zhong , Xiaowei Yang , Qi Lai , Shaorong Huang , Xiaoyong Zhang , Bin Zhang , Yen Wei","doi":"10.1016/j.colsurfb.2025.114495","DOIUrl":"10.1016/j.colsurfb.2025.114495","url":null,"abstract":"<div><div>Natural compounds have shown promising application prospects in preventing or treating various diseases, including osteoporosis on account of their abundant sources, low price, multi-targeting and multiple biological effects. As a bioactive natural product, quercetin (Que) has previously demonstrated to ameliorate osteoporosis (OP), however, its poor bioavailability resulting from low water solubility, poor stability and lack of bone-targeting largely restricted its efficacy and clinical applications. Inspired by the bone-targeting capability of phosphate compounds, we reported a one-step procedure for synthesis of phosphorylated Que (p-Que) by direct phosphorylating phenol groups of Que for the first time. The phosphate groups on p-Que could not only improve the water dispersibility of Que, but also endow p-Que desirable bioavailability and bone-targeting feature. The results from biological assays suggested that p-Que could inhibit osteoclastogenesis and bone resorption and alleviate trabeculae loss in osteoporotic mice. In conclusion, this work demonstrated that phosphorylation strategy can effectively solve low water solubility, lack of bone-targeting capability and poor bioavailability of natural compounds, providing a novel and efficient approach for development of OP nanomedicines.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114495"},"PeriodicalIF":5.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced MRSA-infected wound healing using tannic acid cross-linked carboxymethyl chitosan/polyglutamic acid hydrogel for carbazole Delivery 使用鞣酸交联羧甲基壳聚糖/聚谷氨酸水凝胶进行卡巴唑给药，促进 MRSA 感染伤口的愈合。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-01-02 DOI: 10.1016/j.colsurfb.2024.114490

Soureh Sadat Mirzamani , Mohammad Reza Farahpour , Zohreh Ghazi Tabatabaei

The rampant use of commercial antibiotics not only increases drug resistance but also causes a significant threat to human health. This study assessed the wound healing efficacy of hydrogels crafted from carboxymethyl chitosan (Cmc), polyglutamic acid (γ-PGA), tannic acid (TA), and carbazole (Car), with the aim of expediting the wound healing process. Hydrogels were formulated using Cmc/γ-PGA, Cmc/γ-PGA/TA, and Cmc/γ-PGA/TA/Car, followed by a thorough evaluation of their physicochemical attributes. Additionally, assessments encompassed cytotoxicity, antibacterial efficacy, wound contraction rates, histopathological parameters, immunofluorescent staining of CD31, CD86, and COL1A, along with the determination of serum concentrations of IL-1β, IL-6, and IL-10. The physicochemical analyses validated the successful synthesis of the hydrogels, which exhibited both safety and potent antibacterial properties. Topical application of Cmc/γ-PGA/TA/Car hydrogels notably accelerated wound contraction, as evidenced by heightened expression of CD31 and COL1A, alongside reduced serum concentrations of IL-1β and IL-6. In essence, the Cmc/γ-PGA/TA/Car hydrogel demonstrated a dual effect of mitigating inflammation and modulating the proliferative phase, that shows their abilities for application in the wound healing process.

商业抗生素的猖獗使用不仅增加了耐药性，而且对人类健康造成了重大威胁。本研究评估了羧甲基壳聚糖（Cmc）、聚谷氨酸（γ-PGA）、单宁酸（TA）和咔唑（Car）制备的水凝胶的伤口愈合效果，旨在加速伤口愈合过程。采用Cmc/γ-PGA、Cmc/γ-PGA/TA和Cmc/γ-PGA/TA/Car配制水凝胶，并对其理化性质进行了全面评价。此外，评估包括细胞毒性、抗菌效果、伤口收缩率、组织病理学参数、CD31、CD86和COL1A的免疫荧光染色，以及血清IL-1β、IL-6和IL-10浓度的测定。物理化学分析证实了水凝胶的成功合成，该水凝胶具有安全性和强抗菌性。局部应用Cmc/γ-PGA/TA/Car水凝胶可显著加速创面收缩，CD31和COL1A表达升高，血清IL-1β和IL-6浓度降低。从本质上说，Cmc/γ-PGA/TA/Car水凝胶具有减轻炎症和调节增殖期的双重作用，显示了它们在伤口愈合过程中的应用能力。

{"title":"Enhanced MRSA-infected wound healing using tannic acid cross-linked carboxymethyl chitosan/polyglutamic acid hydrogel for carbazole Delivery","authors":"Soureh Sadat Mirzamani , Mohammad Reza Farahpour , Zohreh Ghazi Tabatabaei","doi":"10.1016/j.colsurfb.2024.114490","DOIUrl":"10.1016/j.colsurfb.2024.114490","url":null,"abstract":"<div><div>The rampant use of commercial antibiotics not only increases drug resistance but also causes a significant threat to human health. This study assessed the wound healing efficacy of hydrogels crafted from carboxymethyl chitosan (Cmc), polyglutamic acid (γ-PGA), tannic acid (TA), and carbazole (Car), with the aim of expediting the wound healing process. Hydrogels were formulated using Cmc/γ-PGA, Cmc/γ-PGA/TA, and Cmc/γ-PGA/TA/Car, followed by a thorough evaluation of their physicochemical attributes. Additionally, assessments encompassed cytotoxicity, antibacterial efficacy, wound contraction rates, histopathological parameters, immunofluorescent staining of CD31, CD86, and COL1A, along with the determination of serum concentrations of IL-1β, IL-6, and IL-10. The physicochemical analyses validated the successful synthesis of the hydrogels, which exhibited both safety and potent antibacterial properties. Topical application of Cmc/γ-PGA/TA/Car hydrogels notably accelerated wound contraction, as evidenced by heightened expression of CD31 and COL1A, alongside reduced serum concentrations of IL-1β and IL-6. In essence, the Cmc/γ-PGA/TA/Car hydrogel demonstrated a dual effect of mitigating inflammation and modulating the proliferative phase, that shows their abilities for application in the wound healing process.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114490"},"PeriodicalIF":5.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0