Colloids and Surfaces B: Biointerfaces最新文献_第3页

Cascade catalytic nanoreactor based on electronic regulatory engineering and biomimetic mineralization strategy for effectively combat bacterial infection in diabetic wounds 基于电子调控工程和仿生矿化策略的级联催化纳米反应器有效对抗糖尿病创面细菌感染。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-03 DOI: 10.1016/j.colsurfb.2025.115345

Xin Wang , Lihua Li , Ke Xu , Xin Li , Bixia Lin , Li Wang

Rapid healing of diabetic wounds remains a huge challenge owing to high blood glucose, susceptibility to bacterial infection and other complex microenvironment. Herein, we cleverly design a glucose-activated multifunctional integrated cascade catalytic nanoreactor for effective combating bacterial infection in diabetic wounds. As a proof of the concept, prussian blue, Rh nanoparticles, and glucose oxidase constitute an activatable nanoreactor "GPR-2.0", which can be activated by glucose on the surface of infected wounds to enhance peroxidase-mimic activity by regulating local pH and self-supplying H₂O₂, thereby continuously producing toxic •OH for sterilization. Notably, in this “intelligent” system, GPR-2.0 not only cuts off the energy supply necessary for bacterial survival via starving therapy, but also destroys the antioxidant protection system of the bacteria by depleting glutathione. When the NIR laser is introduced, the photothermal effect of nanoreactor further enhances the POD-mimic activity, thus amplifying the effect of the chemodynamic therapy (CDT). By this innovative multiple-enhanced CDT and multimodal combined modality, the GPR-2.0 nanoreactor demonstrates extraordinary antimicrobial performance in vitro simulation and diabetic wounds healing assays. To our knowledge, this strategy for direct modulation of the local microenvironment is rarely reported in the antimicrobial field, which promises to start an exciting research direction.

由于高血糖、易受细菌感染和其他复杂的微环境，糖尿病伤口的快速愈合仍然是一个巨大的挑战。在此，我们巧妙地设计了葡萄糖激活的多功能集成级联催化纳米反应器，用于有效对抗糖尿病伤口的细菌感染。为了证明这一概念，普鲁士蓝、Rh纳米粒子和葡萄糖氧化酶组成了一个可活化的纳米反应器“GPR-2.0”，它可以被感染伤口表面的葡萄糖激活，通过调节局部pH和自供H2O2来增强过氧化物酶模拟活性，从而持续产生有毒的•OH用于杀菌。值得注意的是，在这个“智能”系统中，GPR-2.0不仅通过饥饿疗法切断了细菌生存所需的能量供应，还通过消耗谷胱甘肽破坏了细菌的抗氧化保护系统。当引入近红外激光时，纳米反应器的光热效应进一步增强了pod模拟活性，从而放大了化学动力治疗（CDT）的效果。通过这种创新的多重增强CDT和多模态联合模态，GPR-2.0纳米反应器在体外模拟和糖尿病伤口愈合试验中表现出非凡的抗菌性能。据我们所知，这种直接调节局部微环境的策略在抗菌领域很少有报道，这有望开启一个令人兴奋的研究方向。

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引用次数: 0

Design strategy for surface hydrogel coatings on metal biomedical devices based on interfacial bonding mechanisms 基于界面键合机制的金属生物医学器件表面水凝胶涂层设计策略。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-03 DOI: 10.1016/j.colsurfb.2025.115335

Ke Xu , Meihan Liu , Gaolong Yuan , Bo Wang , Zeyu Zhang , Hongji Guo , Dan Dang , Tianming Zhao

With the rapid development of biomedical engineering, metal biomedical devices have attracted considerable attention for their wide range of applications, including neuromonitoring, cardiovascular therapy, and tissue repair. However, the difference in mechanical modulus between the device's metal substrate and human tissues often causes mechanical mismatch during use, leading to inflammation, tissue damage, and reduced long-term performance. To address this issue, researchers have recently proposed constructing hydrogel coatings on metal surfaces, which enhance the tissue integration of devices by combining the mechanical advantages of metals with the biocompatibility and functionality of hydrogels. In this paper, we systematically review two approaches for constructing hydrogel coatings on metal biomedical devices based on interfacial bonding mechanisms: surface bridging and surface initiation. We discuss the formation mechanisms of these coatings and examine their applications in devices such as implantable electronic devices, vascular stents, and medical guidewires. These studies provide an important theoretical foundation and technical support for the development of high-performance, multifunctional medical devices, thereby driving innovation in biomedical engineering.

随着生物医学工程的飞速发展，金属生物医学器件因其在神经监测、心血管治疗、组织修复等方面的广泛应用而备受关注。然而，设备的金属基板和人体组织之间的机械模量差异经常在使用过程中导致机械不匹配，导致炎症、组织损伤和长期性能降低。为了解决这个问题，研究人员最近提出在金属表面构建水凝胶涂层，通过将金属的机械优势与水凝胶的生物相容性和功能相结合，增强设备的组织整合。本文系统综述了基于界面键合机制构建金属生物医学器件水凝胶涂层的两种方法：表面桥接和表面引发。我们讨论了这些涂层的形成机制，并研究了它们在植入式电子设备、血管支架和医疗导丝等设备中的应用。这些研究为高性能、多功能医疗器械的开发提供了重要的理论基础和技术支撑，从而推动了生物医学工程的创新。

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引用次数: 0

Study on antibacterial lubricating coatings for oral implants based on electrostatic self-assembly and Schiff base reaction 基于静电自组装和席夫碱反应的口腔种植体抗菌润滑涂层研究。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115343

Chengxiong Lin , Yinglin Chu , Fei Liu , Panpan Wang

Bacterial infection at the surface of oral implants is one of the primary reasons for surgical failure. To counter the risk of biofilm infection on oral implants and prevent the emergence of drug-resistant bacteria, an increasing number of researchers are focusing on the study of surface coatings for oral implants. Therefore, an antibacterial hydrophilic coating was developed in this study. This coating combines a biomimetic adhesive (dopamine-modified oxidized hyaluronic acid) with a long-acting drug-loaded carrier (aminated mesoporous silica particles) on a polyurethane material through electrostatic self-assembly and a Schiff base reaction. The results showed that the drug-loaded mesoporous silica nanoparticles prepared by the solvent template method had a diameter of approximately 200 nm and a pore size of about 3 nm. The drug could be continuously released in vitro for more than 9 days. Dopamine-modified hyaluronic acid can firmly adhere the drug-loaded mesoporous silica nanoparticles to the material surface, forming a uniform and stable coating. Additionally, the composite coating exhibited effective antibacterial properties against S. aureus and E. coli. The CCK-8 and fluorescence staining results showed that the coating had excellent biocompatibility.

口腔种植体表面细菌感染是导致手术失败的主要原因之一。为了应对口腔种植体生物膜感染的风险，防止耐药菌的出现，越来越多的研究者开始关注口腔种植体表面涂层的研究。因此，本研究开发了一种抗菌亲水性涂料。该涂层通过静电自组装和席夫碱反应将仿生粘合剂（多巴胺修饰的氧化透明质酸）与长效载药载体（胺化介孔二氧化硅颗粒）结合在聚氨酯材料上。结果表明，溶剂模板法制备的载药介孔二氧化硅纳米颗粒直径约为200 nm，孔径约为3 nm。该药物可在体外连续释放9天以上。多巴胺修饰的透明质酸可以将载药的介孔二氧化硅纳米颗粒牢固地粘附在材料表面，形成均匀稳定的涂层。此外，复合涂层对金黄色葡萄球菌和大肠杆菌具有有效的抗菌性能。CCK-8和荧光染色结果表明该涂层具有良好的生物相容性。

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引用次数: 0

Mechanism of biomimetic virus-like nanoparticles in triggering immune response elucidated by proteomics 蛋白质组学研究仿生病毒样纳米颗粒引发免疫反应的机制。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115338

Jiajia Wang , Ahmed Montaser , Minna Sivonen , Henri Leinonen , Lucas Nygård , Tuure Kinnunen , Sini Pitkänen , Anna-Liisa Levonen-Harju , Enkhzaya Batnasan , Leena Latonen , Jussi Hepojoki , Leyuan Ma , Vesa-Pekka Lehto , Wujun Xu

The use of biomimetic nanoparticles (NPs) with virus-like morphology have recently attracted research interest as novel delivery platforms and immune adjuvants. However, the exact interactions between the nanoparticles and immune cells as well as the mechanism involved are not known in detail. This motivated us to develop virus-like mesoporous silica nanoparticles (VLP) to characterize their physicochemical properties, and to determine the immune pathways induced by the particles in mouse macrophages. The results showed inclusion of spikes mimicking viral structures on the surface increased cellular uptake and enhanced immune response as compared to spherical NPs. Proteomic analysis revealed that the pathways of RIG-I-like receptor signaling, MAPK, NF-κB, and Toll-like receptor signaling were involved in regulating the immune response when macrophages interacted with VLP. When the spikes increased from 5 to 30 nm, the expression of immune-related proteins including TRAF6 and PIAS4 proteins was upregulated. This study revealed the interaction pathways and key proteins in the activation of immune response with VLP, which may provide insights to develop novel immunotherapy for enhanced efficacy.

具有病毒样形态的仿生纳米颗粒（NPs）作为一种新的给药平台和免疫佐剂最近引起了人们的研究兴趣。然而，纳米颗粒与免疫细胞之间的确切相互作用以及所涉及的机制尚不清楚。这促使我们开发病毒样介孔二氧化硅纳米颗粒（VLP），以表征其物理化学性质，并确定颗粒在小鼠巨噬细胞中诱导的免疫途径。结果表明，与球形NPs相比，在表面包含模拟病毒结构的尖刺增加了细胞摄取和增强的免疫反应。蛋白质组学分析显示，巨噬细胞与VLP相互作用时，rig - i样受体信号通路、MAPK、NF-κB和toll样受体信号通路参与调节免疫应答。当峰值从5到30 nm增加时，免疫相关蛋白TRAF6和PIAS4蛋白的表达上调。本研究揭示了VLP与免疫应答激活的相互作用途径和关键蛋白，为开发新的免疫疗法提供了新的思路。

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引用次数: 0

Sustainable encapsulation of lipophilic fragrances using biodegradable sodium alginate for cosmetic applications 使用可生物降解的海藻酸钠的化妆品应用的亲脂性香料的可持续封装

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115341

Anna Tarrats-Duran, Salvador Borrós, José Rodrigo Magaña, Cristina Fornaguera

The encapsulation of lipophilic compounds for use in the cosmetic, food, and detergency industries is an area of growing interest. However, most current strategies rely on non-biodegradable materials, often classified as microplastics, which pose significant environmental risks. To address these issues, alternative encapsulation methods using biodegradable materials are being developed. Despite their potential, these methods have yet to demonstrate efficacy or economic feasibility comparable to conventional encapsulation systems. To overcome these challenges, a novel strategy has been developed for the encapsulation of lipophilic compounds, such as fragrances, using sodium alginate (SA). This approach involves the formation of an oil-in-water nanoemulsion via the Phase Inversion Composition method, with polysorbate 80 serving as the surfactant. The process is followed by the internal gelation of SA and subsequent dispersion to generate the final microcapsules. The formulation was optimized by varying the ratios of surfactant, oil, and aqueous phases in the nanoemulsion. Characterization techniques, including Dynamic Light Scattering, Gas Chromatography–Mass Spectrometry, and Thermogravimetric Analysis, confirmed successful encapsulation (average of 81 %, up to 97 % for one fragrance). The formulations demonstrated prolonged release profiles, with the scent remaining detectable for up to 30 days. An organoleptic study further revealed that encapsulated fragrances retained higher perceived intensity over time compared to their non-encapsulated counterparts. Moreover, the microcapsules exhibited excellent long-term stability within a conditioner matrix, maintaining their fragrance load for four months. This work represents a significant advancement in the development of environmentally friendly encapsulation methods for lipophilic compounds, offering promising applications in the cosmetic industry.

用于化妆品、食品和洗涤剂工业的亲脂化合物的包封是一个日益引起人们兴趣的领域。然而，目前的大多数策略依赖于不可生物降解的材料，这些材料通常被归类为微塑料，它们构成了重大的环境风险。为了解决这些问题，正在开发使用可生物降解材料的替代封装方法。尽管具有潜力，但这些方法尚未证明与传统封装系统相比的有效性或经济可行性。为了克服这些挑战，研究人员开发了一种新的策略，使用海藻酸钠（SA）包封亲脂性化合物，如芳香剂。该方法包括通过相反转组成法形成水包油纳米乳液，聚山梨酯80作为表面活性剂。这个过程之后是SA的内部凝胶化和随后的分散，以产生最终的微胶囊。通过改变纳米乳中表面活性剂、油和水相的比例，优化了配方。表征技术，包括动态光散射、气相色谱-质谱和热重分析，证实了成功的包封（平均81 %，最高97 %）。这些配方显示出较长的释放时间，气味可保持30天。一项感官研究进一步表明，与未封装的香水相比，封装的香水随着时间的推移保留了更高的感知强度。此外，微胶囊在调理剂基质中表现出优异的长期稳定性，可保持4个月的香味负荷。这项工作代表了亲脂性化合物的环保封装方法的重大进展，在化妆品工业中提供了有前途的应用。

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引用次数: 0

Preparation of thymol-loaded Pickering emulsion gel film and its application in wound healing 载胸腺皮克林乳凝胶膜的制备及其在伤口愈合中的应用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115342

Yan Hu , Jiasi Zhao , Hudie Fu, Shengpeng Zhu, Yuqing Wang, Fengning Lu, Lirong Huang, Shanwei Long, Xiaojun Li, Li Kang, Xinzhou Yang

At present, the treatment of diabetic wounds is challenging and time-consuming. In this study, a delivery system combining thymol-loaded Pickering emulsion and gel film has been investigated to accelerate wound healing. The nanoparticles (SPI-CMC) composed of soy protein isolate (SPI) and carboxymethyl cellulose (CMC) were used to stabilize Pickering emulsion and loaded into polyvinyl alcohol (PVA)-porous starch (PS)-hyaluronic acid (HA) gel membrane (PPH) matrix. The PPH matrix was loaded with thymol (PPH+T), which effectively solves the problem of solubility and compatibility of thymol. Moreover, the PPH+T matrix based on Pickering emulsion and gel membrane achieving the antibacterial, anti-inflammatory, angiogenesis and tissue collagen formation effect. The composite gel membrane exhibited a long-term sustained release of thymol for up to 48 h, and had good antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). The antibacterial diameters of the two were 19.73 ± 1.375 mm and 21.94 ± 0.486 mm, respectively. It was verified in animal experiments that the healing of normal wounds and diabetic wounds was accelerated, and the final healing rates were 97 % and 94 %, respectively. Therefore, the composite gel membrane prepared in this study has great potential in wound antibacterial and healing, and provides a basis for effective treatment of chronic wounds.

目前，糖尿病伤口的治疗具有挑战性和耗时。在这项研究中，我们研究了一种将百里香皮克林乳剂与凝胶膜结合的递送系统，以加速伤口愈合。用大豆分离蛋白（SPI）和羧甲基纤维素（CMC）组成的纳米颗粒（SPI-CMC）稳定Pickering乳状液，并将其装入聚乙烯醇(PVA)-多孔淀粉(PS)-透明质酸（HA）凝胶膜（PPH）基质中。在PPH基质中加入百里香酚（PPH+T），有效地解决了百里香酚的溶解度和相容性问题。此外，基于皮克林乳剂和凝胶膜的PPH+T基质具有抗菌、抗炎、血管生成和组织胶原形成的作用。复合凝胶膜对百里香酚的缓释时间长达48 h，对大肠杆菌（E.coli）和金黄色葡萄球菌（s.d aureus）具有良好的抑菌活性。两者的抑菌直径分别为19.73 ±1.375 mm和21.94 ±0.486 mm。动物实验证实，正常创面和糖尿病创面的愈合速度加快，最终愈合率分别为97 %和94 %。因此，本研究制备的复合凝胶膜在伤口抗菌和愈合方面具有很大的潜力，为有效治疗慢性伤口提供了基础。

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引用次数: 0

A biodegradable chitosan-nano-hydroxyapatite aerogel promotes angiogenesis and osteogenesis for skull defect repair 可生物降解壳聚糖-纳米羟基磷灰石气凝胶促进颅骨缺损修复血管生成和骨生成

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115339

Xiaohua Su , Wenqiang Li , Renzhao Huang , Jingting Huang , Wenyong Huang , Xide Chen , Xiaodong Wu , Jianfeng Li , Xinting Li , Deguang Qin , Yi Zhan

The course of the skull defect is long, with multiple factors involved. Traditional treatment methods are not sufficient to completely restore the skull defect in a short period. Biomaterial scaffold could replace traditional approaches to bone reconstruction. A major challenge in designing a scaffold for cranial defect repair is to be non-toxic, low swelling, biodegradable, and pro-osteogenic. Herein, a biodegradable aerogel scaffold composed of methylcellulose (MC), thiolated chitosan (TCS), Zn^2 + and nano hydroxyapatite (nHA) was designed for repairing the skull defect. MC/TCS-nHA aerogel was formed by the ionic cross-linking of hydroxyl, amino and thiol groups with Zn²⁺. The prepared scaffolds exhibited a uniform pore structure and structural stability. Interestingly, TCS was used to enhance biocompatibility and biodegradability, Zn²⁺ to enhance antibacterial properties and nHA to enhance pro-osteogenicity. In vivo and in vitro tests confirmed that chitosan-based aerogel could promote macrophage polarization into the M2 phenotype and promote angiogenesis and osteogenesis. The micro-CT characterization analysis and immunofluorescence analysis after 12 weeks indicated that MC/TCS-nHA aerogel could accelerate skull defect repair. Therefore, the chitosan-based aerogel showed great promise as an implant material for medical applications involving the repair of the skull.

颅骨缺损病程长，涉及多种因素。传统的治疗方法不足以在短时间内完全修复颅骨缺损。生物材料支架可以替代传统的骨重建方法。设计用于颅骨缺损修复的支架的主要挑战是无毒、低肿胀、可生物降解和促骨。本文设计了一种由甲基纤维素（MC）、巯基壳聚糖（TCS）、Zn2 +和纳米羟基磷灰石（nHA）组成的可生物降解气凝胶支架用于修复颅骨缺损。MC/TCS-nHA气凝胶是由羟基、氨基和巯基与Zn2+离子交联形成的。制备的支架具有均匀的孔隙结构和结构稳定性。有趣的是，TCS用于增强生物相容性和生物降解性，Zn2+用于增强抗菌性能，nHA用于增强促骨性。体内和体外实验证实壳聚糖基气凝胶能促进巨噬细胞向M2型极化，促进血管生成和成骨。12周后显微ct表征分析和免疫荧光分析表明，MC/TCS-nHA气凝胶可以促进颅骨缺损的修复。因此，壳聚糖为基础的气凝胶显示了巨大的前景，作为一种植入材料的医学应用涉及颅骨修复。

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引用次数: 0

A Hierarchical core-shell nanohybrid for dual-stimuli-responsive antifungal delivery against Botrytis cinerea 一种具有双重刺激反应的核壳级纳米杂化物抗葡萄孢。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-02 DOI: 10.1016/j.colsurfb.2025.115340

Qi Sun , Jian Zeng , Miaomiao Ma , Meiqi Huang , Jianmei Li , Tao Le

The persistent threat of phytopathogenic fungi necessitates precision-targeted and sustainable antifungal strategies beyond conventional agrochemicals. This study presents a hierarchically structured micro/nanosystem (CMC@Eu-ZIF@ZnO) engineered for precision antifungal delivery by leveraging the specific virulence mechanisms of Botrytis cinerea. The composite features a dual core-shell architecture: a ZnO microsphere core encapsulated within a ZIF-8 intermediate shell and an outer carboxymethyl cellulose (CMC) coating. This intelligent design integrates pH- and cellulase-triggered release pathways, exploiting fungal-induced and enzymatic secretion for on-demand cargo delivery. Synergistic action between Eu and ZnO nanoparticles enhances membrane disruption and oxidative stress, resulting in superior antifungal activity. The system achieved 91.5 % cumulative release under dual stimuli and 67.9 % rainfast retention, with negligible phytotoxicity in soybean germination assays and effective lesion control in tomato leaves. By overcoming instability, non-target leakage, and limited responsiveness of conventional fungicides, this study establishes a microenvironment-responsive hierarchical delivery platform. This work highlights the potential of integrating natural antifungals with hierarchical nanostructures for eco-friendly, precision crop protection.

植物致病性真菌的持续威胁需要精确靶向和可持续的抗真菌策略，而不是传统的农用化学品。本研究提出了一种分层结构的微/纳米系统（CMC@Eu-ZIF@ZnO），通过利用灰葡萄孢的特定毒力机制，设计用于精确抗真菌递送。该复合材料具有双核-壳结构：ZnO微球芯封装在ZIF-8中间壳内，外层是羧甲基纤维素（CMC）涂层。这种智能设计整合了pH值和纤维素酶触发的释放途径，利用真菌诱导和酶促分泌来按需运送货物。Eu和ZnO纳米粒子之间的协同作用增强了膜破坏和氧化应激，从而产生优异的抗真菌活性。该系统在双重刺激下的累积释放率为91.5%，防雨保留率为67.9%，对大豆萌发试验的植物毒性可以忽略不计，对番茄叶片的病害控制有效。通过克服传统杀菌剂的不稳定性、非靶标泄漏和有限的响应性，本研究建立了微环境响应的分级给药平台。这项工作强调了将天然抗真菌剂与分层纳米结构结合起来用于生态友好、精确的作物保护的潜力。

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引用次数: 0

Bismuth based nanomaterials: Design, synthesis and applications in tumor synergistic therapy. 铋基纳米材料：设计、合成及其在肿瘤协同治疗中的应用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.colsurfb.2025.114992

Qiupeng Zhang, Jie Tu, Yuqin Jiang, Huihui Wang, Weixian Xue, Chunyao Dong, Ruizhuo Ouyang, Yuqing Miao

With the rapid development of nanomedicine, nanomaterials (NMs) have been used in clinical medicine to improve treatment efficiency. Bismuth-based nanomaterials(BNMs) are promising candidates for cancer diagnosis and therapy because of their good biocompatibility, low cost, high X-ray attenuation and near infrared absorption coefficient. The traditional therapy mode is relatively single, and the treatment effect is not significantly improved. Therefore, the combination of traditional treatment and emerging treatment not only has high lethality to tumor cell, but also makes up for their shortcomings. This review summarizes the latest research on BNMs in tumor therapy in recent years, including: the design and preparation of BNMs, the synergy of multiple treatment technologies, and the biosafety of nanomaterials. In the end, the inspiration and reference for the study of Bi-based multifunctional nano-platforms were provided for clinical use.

随着纳米医学的快速发展，纳米材料已被广泛应用于临床医学，以提高治疗效率。铋基纳米材料具有良好的生物相容性、低成本、高x射线衰减和近红外吸收系数等优点，在癌症诊断和治疗中具有广阔的应用前景。传统的治疗模式比较单一，治疗效果没有明显提高。因此，传统治疗与新兴治疗相结合，不仅对肿瘤细胞具有较高的致死率，而且弥补了各自的不足。本文综述了近年来生物纳米材料在肿瘤治疗中的最新研究进展，包括生物纳米材料的设计与制备、多种治疗技术的协同作用、纳米材料的生物安全性等。最后，为bi基多功能纳米平台的研究提供了启示和参考。

引用次数: 0

Quaternized chitosan-coated PLGA nanoparticles co-deliver resveratrol and all-trans retinoic acid to enhance humoral immunity, cellular immunity and gastrointestinal mucosal immunity. 季铵盐化壳聚糖包被的PLGA纳米颗粒共同递送白藜芦醇和全反式维甲酸，增强体液免疫、细胞免疫和胃肠道粘膜免疫。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.colsurfb.2025.114994

Dan Yang, Nannan Wang, Lijuan Cao, Huimao Liu, Hanyan Cheng, Haitao Ma, Lixia Li, Yuanfeng Zou, Xinghong Zhao, Xun Zhou, Xu Song, Dongmei Zhang, Mingyue Li, Renyong Jia, Zhongqiong Yin

Conventional vaccine adjuvants are limited by their mechanisms of action and administration routes, often failing to simultaneously elicit robust systemic and mucosal immune responses. This limitation compromises the establishment of dual protective barriers during early pathogen invasion. Therefore, we developed an innovative poly (lactic-co-glycolic acid) (PLGA) nanoparticle-based adjuvant system (Res/RA QCS NPs) featuring quaternized chitosan (QCS) surface modification for ovalbumin (OVA) delivery, co-encapsulating dual immunomodulators - resveratrol (Res) and all-trans retinoic acid (RA). The results showed that the Res/RA QCS NPs possessed excellent antigen adsorption capacity (adsorption rate of 93.87 ± 5.27 %) and significantly enhanced the recruitment of antigen-presenting cells (APCs) at the injection site and lymph node-targeting delivery. In vitro immunological evaluation further confirmed that OVA-Res/RA QCS NPs possessed excellent immune-enhancing properties, including: efficient antigen internalization, Dendritic Cells (DCs) maturation activation, enhanced cytokine secretion and mucosal homing ability. In vivo immunity experiments, OVA-Res/RA QCS NPs not only induced high levels of serum antigen-specific IgG antibodies and proliferation and activation of T cells in peripheral lymphoid tissues, but also stimulated the secretion of large amounts of antigen-specific IgA in the gastrointestinal mucosa, which realized the dual activation of systemic and mucosal immunity. This study provides an important theoretical and experimental basis for the development of novel vaccine adjuvants and immunotherapy strategies based on intestinal immunomodulation.

传统的疫苗佐剂受到其作用机制和给药途径的限制，往往不能同时引起强大的全身和粘膜免疫反应。这种限制损害了在早期病原体入侵期间建立双重保护屏障。因此，我们开发了一种创新的聚乳酸-羟基乙酸（PLGA）纳米颗粒佐剂体系（Res/RA QCS NPs），该体系采用季铵盐壳聚糖（QCS）表面改性，用于卵清蛋白（OVA）的递送，共包封双重免疫调节剂-白藜芦醇（Res）和全反式维甲酸（RA）。结果表明，Res/RA QCS NPs具有优异的抗原吸附能力（吸附率为93.87 ± 5.27 %），显著增强了抗原提呈细胞（APCs）在注射部位的募集和淋巴结靶向递送。体外免疫评价进一步证实OVA-Res/RA QCS NPs具有良好的免疫增强特性，包括：高效的抗原内化，激活树突状细胞（dc）成熟，增强细胞因子分泌和粘膜归巢能力。在体内免疫实验中，OVA-Res/RA QCS NPs不仅能诱导高水平的血清抗原特异性IgG抗体和外周淋巴组织T细胞的增殖活化，还能刺激胃肠道黏膜分泌大量抗原特异性IgA，实现全身免疫和粘膜免疫的双重激活。本研究为开发基于肠道免疫调节的新型疫苗佐剂和免疫治疗策略提供了重要的理论和实验依据。

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引用次数: 0