Pub Date : 2025-02-19DOI: 10.1016/j.colsurfb.2025.114574
Siying Cheng , Hongliang Wang , Xiaoqian He , Yun Shao , Fengguang Ma , Jianan Huang , Bing Hu , Zhonghua Liu
Food hydrogels targeting respiration of microorganisms via changing the micro-ecological environment in gut were prepared through the self-assembly of polyphenols extracted from tea leaves harvested in summer and autumn and the protein fibrils originating from egg white lysozyme. Oral administration with the hydrogels effectively inhibited the over-expansion of the facultative anaerobic bacterium indicated by E. coli Nissle 1917 (EcN) and alleviated the clinic symptoms of chronic intestinal inflammation in mice. Importantly, the hypoxia of epithelial cells was elevated significantly and the overexpression of the inducible NO synthase (INOs)-related NOS2 gene was inhibited substantially in colons of the colitis mice, which accounted for prevention of the abnormal expansion of E. coli via blocking respiration. The treatment with the hydrogels preserved normal mitochondrial function in colonic epithelial cells under oxidative stress, which could serve as the mechanism to maintain the capability to consume oxygen.
{"title":"Hydrogels of diet-derived electron donors restore epithelial hypoxia and reduce iNOS synthesis to inhibit inflammation-induced overgrowth of facultatively anaerobic bacteria for gut homeostasis","authors":"Siying Cheng , Hongliang Wang , Xiaoqian He , Yun Shao , Fengguang Ma , Jianan Huang , Bing Hu , Zhonghua Liu","doi":"10.1016/j.colsurfb.2025.114574","DOIUrl":"10.1016/j.colsurfb.2025.114574","url":null,"abstract":"<div><div>Food hydrogels targeting respiration of microorganisms via changing the micro-ecological environment in gut were prepared through the self-assembly of polyphenols extracted from tea leaves harvested in summer and autumn and the protein fibrils originating from egg white lysozyme. Oral administration with the hydrogels effectively inhibited the over-expansion of the facultative anaerobic bacterium indicated by <em>E. coli</em> Nissle 1917 (EcN) and alleviated the clinic symptoms of chronic intestinal inflammation in mice. Importantly, the hypoxia of epithelial cells was elevated significantly and the overexpression of the inducible NO synthase (INOs)-related NOS2 gene was inhibited substantially in colons of the colitis mice, which accounted for prevention of the abnormal expansion of <em>E. coli via</em> blocking respiration. The treatment with the hydrogels preserved normal mitochondrial function in colonic epithelial cells under oxidative stress, which could serve as the mechanism to maintain the capability to consume oxygen.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114574"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-18DOI: 10.1016/j.colsurfb.2025.114575
Jingna Wu , Bangfeng Lin , Nan Pan , Xiaoting Chen , Bei Chen , Mingming Hou , Xiaoya Qu
Tachyplesin II (TP) is a β-folded peptide isolated from horseshoe crabs. TP, with two disulfide bonds, effectively inhibits gram-negative and gram-positive bacteria, fungi and viruses. In this study, the antibacterial and antioxidant properties of synthetic TP were examined. Specifically, TP was incorporated into cross-linked methacrylated gelatine (GelMA) to construct a GelMA-TP hydrogel, which promoted infected wound healing. TP formed intermolecular hydrogen bonds with the GelMA network, endowing the GelMA-TP hydrogel with good antibacterial activity, stable rheological properties, high swelling capacity, self-healing behaviour, and good biocompatibility. We found that the anti-inflammatory and antioxidant activities of the GelMA-TP hydrogel significantly enhanced collagen production, thus accelerating healing in a rat-infected wound model. Overall, the GelMA-TP hydrogel demonstrates significant potential to stimulate the healing of infected wounds by reducing healing time and improving tissue repair outcomes. These findings highlight its translational promise as a clinically effective material for managing complex wounds, particularly in scenarios where conventional therapies are limited by persistent infections or excessive inflammation.
{"title":"Construction of a methacrylated gelatine composite hydrogel based on tachyplesin II and its application in the repair of infected wounds","authors":"Jingna Wu , Bangfeng Lin , Nan Pan , Xiaoting Chen , Bei Chen , Mingming Hou , Xiaoya Qu","doi":"10.1016/j.colsurfb.2025.114575","DOIUrl":"10.1016/j.colsurfb.2025.114575","url":null,"abstract":"<div><div>Tachyplesin II (TP) is a β-folded peptide isolated from horseshoe crabs. TP, with two disulfide bonds, effectively inhibits gram-negative and gram-positive bacteria, fungi and viruses. In this study, the antibacterial and antioxidant properties of synthetic TP were examined. Specifically, TP was incorporated into cross-linked methacrylated gelatine (GelMA) to construct a GelMA-TP hydrogel, which promoted infected wound healing. TP formed intermolecular hydrogen bonds with the GelMA network, endowing the GelMA-TP hydrogel with good antibacterial activity, stable rheological properties, high swelling capacity, self-healing behaviour, and good biocompatibility. We found that the anti-inflammatory and antioxidant activities of the GelMA-TP hydrogel significantly enhanced collagen production, thus accelerating healing in a rat-infected wound model. Overall, the GelMA-TP hydrogel demonstrates significant potential to stimulate the healing of infected wounds by reducing healing time and improving tissue repair outcomes. These findings highlight its translational promise as a clinically effective material for managing complex wounds, particularly in scenarios where conventional therapies are limited by persistent infections or excessive inflammation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114575"},"PeriodicalIF":5.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Activation of the cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes (STING) has great potential to promote antitumor immunity. As a major effector of the cell to sense and respond to the aberrant presence of cytoplasmic double-stranded DNA (dsDNA), inducing the expression and secretion of type I interferons (IFN) and STING, cGAS-STING signaling pathway establishes an effective natural immune response, which is one of the fundamental mechanisms of host defense in organisms. In addition to the release of heterologous DNA due to pathogen invasion and replication, mitochondrial damage and massive cell death can also cause abnormal leakage of the body's own dsDNA, which is then recognized by the DNA receptor cGAS and activates the cGAS-STING signaling pathway. However, small molecule STING agonists suffer from rapid excretion, low bioavailability, non-specificity and adverse effects, which limits their therapeutic efficacy and in vivo application. Various types of nano-delivery systems, on the other hand, make use of the different unique structures and surface modifications of nanoparticles to circumvent the defects of small molecule STING agonists such as fast metabolism and low bioavailability. Also, the nanoparticles are precisely directed to the focal site, with their own appropriate particle size combined with the characteristics of passive or active targeting. Herein, combined with the cGAS-STING pathway to activate the immune system and kill tumor tissues directly or indirectly, which help maximize the use of the functions of chemotherapy, photothermal therapy(PTT), chemodynamic therapy(CDT), and radiotherapy(RT). In this review, we will discuss the mechanism of action of the cGAS-STING pathway and introduce nanoparticle-mediated tumor combination therapy based on the STING pathway. Collectively, the effective multimodal nanoplatform, which can activate cGAS-STING pathway for enhanced anti-tumor immunotherapy, has promising avenue clinical applications for cancer treatment.
{"title":"Advances in nanoplatform-based multimodal combination therapy activating STING pathway for enhanced anti-tumor immunotherapy","authors":"Huizhong Zhang, Xiaohan Xu, Shiman Li, Huating Huang, Ke Zhang, Wenjing Li, Xinzhu Wang, Jingwen Yang, Xingbin Yin, Changhai Qu, Jian Ni, Xiaoxv Dong","doi":"10.1016/j.colsurfb.2025.114573","DOIUrl":"10.1016/j.colsurfb.2025.114573","url":null,"abstract":"<div><div>Activation of the cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes (STING) has great potential to promote antitumor immunity. As a major effector of the cell to sense and respond to the aberrant presence of cytoplasmic double-stranded DNA (dsDNA), inducing the expression and secretion of type I interferons (IFN) and STING, cGAS-STING signaling pathway establishes an effective natural immune response, which is one of the fundamental mechanisms of host defense in organisms. In addition to the release of heterologous DNA due to pathogen invasion and replication, mitochondrial damage and massive cell death can also cause abnormal leakage of the body's own dsDNA, which is then recognized by the DNA receptor cGAS and activates the cGAS-STING signaling pathway. However, small molecule STING agonists suffer from rapid excretion, low bioavailability, non-specificity and adverse effects, which limits their therapeutic efficacy and in vivo application. Various types of nano-delivery systems, on the other hand, make use of the different unique structures and surface modifications of nanoparticles to circumvent the defects of small molecule STING agonists such as fast metabolism and low bioavailability. Also, the nanoparticles are precisely directed to the focal site, with their own appropriate particle size combined with the characteristics of passive or active targeting. Herein, combined with the cGAS-STING pathway to activate the immune system and kill tumor tissues directly or indirectly, which help maximize the use of the functions of chemotherapy, photothermal therapy(PTT), chemodynamic therapy(CDT), and radiotherapy(RT). In this review, we will discuss the mechanism of action of the cGAS-STING pathway and introduce nanoparticle-mediated tumor combination therapy based on the STING pathway. Collectively, the effective multimodal nanoplatform, which can activate cGAS-STING pathway for enhanced anti-tumor immunotherapy, has promising avenue clinical applications for cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114573"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.colsurfb.2025.114567
Qifen Li , Leixiang Wang , Yuxin Li , Shukai Nan , Ziqing Tan , Qian Yang , Chengbo Li , Xianrui Xie , Huanhuan Yan , Guige Hou , Shun Duan , Yu-Qing Zhao
We report highly efficient, antioxidant, anti-inflammatory, pH-responsive antibacterial coatings developed via direct assembly of a nature compound tannic acid (TA) with the cationic antibiotic quaternized polyethyleneimine (QPEI). The surface of polypropylene was modified with these coatings. Under acidic conditions, the coatings significantly enhanced the antibacterial performance against Staphylococcus aureus and Escherichia coli, and the antibacterial rate reached more than 90 %. The free radical scavenging rate could exceed 91 %. Thus, the excess reactive oxygen species (ROS) could be cleared, and the oxidative stress production could be significantly reduced. In vitro anti-inflammatory experiments revealed that the coatings significantly reduced the expression of TNF-α and IL-6 and promoted the release of IL-10. These results indicated the excellent anti-inflammatory effects of the coatings. In vivo experiments revealed that the coatings could rapidly achieve bactericidal effects and subsequently prevent inflammatory reactions, thereby inhibiting the generation of fibrosis. Through molecular docking simulation experiments, the mechanism of LBL self-assembly between QPEI and TA components has been clarified for the first time. By designing the surface coating of a material and combining it with bioactive components, multiple functions could be achieved to meet the clinical needs of stoma patches and promote the development of medical materials.
{"title":"Tannic acid coating modification of polypropylene providing pH-responsive antibacterial and anti-inflammatory properties applicable to ostomy patches","authors":"Qifen Li , Leixiang Wang , Yuxin Li , Shukai Nan , Ziqing Tan , Qian Yang , Chengbo Li , Xianrui Xie , Huanhuan Yan , Guige Hou , Shun Duan , Yu-Qing Zhao","doi":"10.1016/j.colsurfb.2025.114567","DOIUrl":"10.1016/j.colsurfb.2025.114567","url":null,"abstract":"<div><div>We report highly efficient, antioxidant, anti-inflammatory, pH-responsive antibacterial coatings developed via direct assembly of a nature compound tannic acid (TA) with the cationic antibiotic quaternized polyethyleneimine (QPEI). The surface of polypropylene was modified with these coatings. Under acidic conditions, the coatings significantly enhanced the antibacterial performance against <em>Staphylococcus aureus</em> and <em>Escherichia coli,</em> and the antibacterial rate reached more than 90 %. The free radical scavenging rate could exceed 91 %. Thus, the excess reactive oxygen species (ROS) could be cleared, and the oxidative stress production could be significantly reduced. <em>In vitro</em> anti-inflammatory experiments revealed that the coatings significantly reduced the expression of TNF-α and IL-6 and promoted the release of IL-10. These results indicated the excellent anti-inflammatory effects of the coatings. <em>In vivo</em> experiments revealed that the coatings could rapidly achieve bactericidal effects and subsequently prevent inflammatory reactions, thereby inhibiting the generation of fibrosis. Through molecular docking simulation experiments, the mechanism of LBL self-assembly between QPEI and TA components has been clarified for the first time. By designing the surface coating of a material and combining it with bioactive components, multiple functions could be achieved to meet the clinical needs of stoma patches and promote the development of medical materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114567"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Traditional transdermal drug delivery methods are often plagued by technical inefficiencies, limited absorption, and the potential for adverse reactions. In contrast, dissolving microneedles (DMNs) offer a novel approach to transdermal drug delivery by effectively merging the benefits of subcutaneous injection with those of conventional transdermal methods. These microneedles dissolve completely within the body, releasing the encapsulated antigen without leaving any sharp remnants. Furthermore, DMNs overcome the limitations of traditional transdermal patches, which are restricted to delivering only small molecule drugs. By facilitating the efficient transdermal absorption of large molecules, DMNs enable precise and painless disease treatment. With advantages such as effective delivery, safety, controllable administration, DMNs hold significant promise in the fields of disease treatment and drug delivery. This article explores the substrate materials, preparation techniques, characterization methods, and current applications of DMNs. We also discuss the current challenges and obstacles faced by DMNs. Finally, we outline potential future research directions for DMNs, aiming to provide a theoretical reference for researchers involved in their preparation and application.
{"title":"Dissolving microneedles: Drug delivery and disease treatment","authors":"Yanling Zhuo , Fangyue Wang , Qizhuang Lv , Chunyan Fang","doi":"10.1016/j.colsurfb.2025.114571","DOIUrl":"10.1016/j.colsurfb.2025.114571","url":null,"abstract":"<div><div>Traditional transdermal drug delivery methods are often plagued by technical inefficiencies, limited absorption, and the potential for adverse reactions. In contrast, dissolving microneedles (DMNs) offer a novel approach to transdermal drug delivery by effectively merging the benefits of subcutaneous injection with those of conventional transdermal methods. These microneedles dissolve completely within the body, releasing the encapsulated antigen without leaving any sharp remnants. Furthermore, DMNs overcome the limitations of traditional transdermal patches, which are restricted to delivering only small molecule drugs. By facilitating the efficient transdermal absorption of large molecules, DMNs enable precise and painless disease treatment. With advantages such as effective delivery, safety, controllable administration, DMNs hold significant promise in the fields of disease treatment and drug delivery. This article explores the substrate materials, preparation techniques, characterization methods, and current applications of DMNs. We also discuss the current challenges and obstacles faced by DMNs. Finally, we outline potential future research directions for DMNs, aiming to provide a theoretical reference for researchers involved in their preparation and application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114571"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.colsurfb.2025.114572
Sara F.C. Guerreiro , Filipa Leal , Anabela G. Dias , Pedro L. Granja , Juliana R. Dias
Gastric wound healing constitutes a complex challenge, even in the context of superficial wounds, due to the harsh stomach environment, characterized by high pH variability and dynamic enzyme concentrations. Therefore, effective healing depends on robust mechanical support and adequate biochemical cues to drive cell growth and proliferation. Electrospun-based dressings may offer a solution to these problems by providing physical support that mimics native extracellular matrix. In this study, electrospun dressings composed of a blend of polycaprolactone (PCL) and gelatin (Gel) were proposed for the first time for gastric application by tuning the relative PCL:Gel ratios (75:25, 50:50 and 25:75) to optimize both their retention capacity and cellular interaction. PCL/Gel dressings, in a proportion of 75:25, showed to have efficient mucoadhesion (ultimate stress of 1.8 MPa) when tested in ex vivo porcine samples. They were also stable in simulated gastric fluid for 14 days, a period compatible with the treatment window. Moreover, the non-cytotoxic biological response (>90 %) of the dressings was favorably validated in mouse fibroblast L929 cell line. Cell morphology, metabolic activity, cell viability and proliferative capacity were assessed using human specific gastric cell lines, including normal stomach fibroblasts (NST-20) and gastric adenocarcinoma (AGS). Overall, PCL/Gel dressings of 75:25 increased the proliferation rate of NST20 and AGS cells after 3 and 7 days in culture, respectively, with significant expression of proliferation marker Ki-67 protein.
{"title":"Polycaprolactone (PCL)‐Gelatin Electrospun Meshes for Accelerated Gastric Wound Healing","authors":"Sara F.C. Guerreiro , Filipa Leal , Anabela G. Dias , Pedro L. Granja , Juliana R. Dias","doi":"10.1016/j.colsurfb.2025.114572","DOIUrl":"10.1016/j.colsurfb.2025.114572","url":null,"abstract":"<div><div>Gastric wound healing constitutes a complex challenge, even in the context of superficial wounds, due to the harsh stomach environment, characterized by high pH variability and dynamic enzyme concentrations. Therefore, effective healing depends on robust mechanical support and adequate biochemical cues to drive cell growth and proliferation. Electrospun-based dressings may offer a solution to these problems by providing physical support that mimics native extracellular matrix. In this study, electrospun dressings composed of a blend of polycaprolactone (PCL) and gelatin (Gel) were proposed for the first time for gastric application by tuning the relative PCL:Gel ratios (75:25, 50:50 and 25:75) to optimize both their retention capacity and cellular interaction. PCL/Gel dressings, in a proportion of 75:25, showed to have efficient mucoadhesion (ultimate stress of 1.8 MPa) when tested in <em>ex vivo</em> porcine samples. They were also stable in simulated gastric fluid for 14 days, a period compatible with the treatment window. Moreover, the non-cytotoxic biological response (>90 %) of the dressings was favorably validated in mouse fibroblast L929 cell line. Cell morphology, metabolic activity, cell viability and proliferative capacity were assessed using human specific gastric cell lines, including normal stomach fibroblasts (NST-20) and gastric adenocarcinoma (AGS). Overall, PCL/Gel dressings of 75:25 increased the proliferation rate of NST20 and AGS cells after 3 and 7 days in culture, respectively, with significant expression of proliferation marker Ki-67 protein.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114572"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.colsurfb.2025.114566
Rui Shi , Xiaotong Wang , Fangli Gang , Jiayu Shi , Shuping Wang , Wanting Liu , Weilong Ye , Xiaodan Sun
Oriented poly(lactic acid) (PLA) fiber bone tissue engineering scaffolds are often limited by factors including poor material hydrophilicity and weak osteogenic activity. The introduction of in situ mineralization can address these issues, but it requires the assistance of hydrophilic materials to achieve optimal performance. Collagen, a nature-based ECM component, was adopted because it can enhance hydrophilicity, encourage cell adhesion, and biomimetrically induce mineralization, according to recent studies of ECM-mimicking scaffolds. Therefore, this study proposes a collagen-mediated in situ mineralization-enhanced scaffold design aimed at improving the hydrophilicity and osteogenic potential of oriented fiber scaffolds. Collagen (5–10 wt%) and phosphate-containing solutions (59.6 mM) were added to a PLA matrix, and scaffolds were electrospun at 12 kV. Subsequently, the scaffolds underwent in situ mineralization in a calcium ion-containing solution (101 mM), leading to the formation of calcium phosphate within the scaffold structure. The experimental results show that the introduction of collagen effectively promoted the formation of in situ mineralization, enhanced the hydrophilicity of the scaffold, and maintained good fiber orientation. The scaffolds exhibited significant mechanical anisotropy, with the Young's modulus parallel to the fiber direction reaching 5 MPa, which is 25 times greater than that in the direction perpendicular to the fibers. In vitro studies with rat bone marrow mesenchymal stem cells showed a 2.4-fold increase in osteogenic differentiation, as assessed by alkaline phosphatase activity. Micro-CT analysis showed that the increase of BV/TV was 3.26 times higher when compared to that of control scaffolds, while histological analysis revealed mature bone tissue formation characterized by well-organized collagen fibers. Overall, the present study describes a novel strategy of collagen-mediated in situ mineralization, first integrating enhanced hydrophilicity, mechanical anisotropy, and biomimetic bone-like properties to address major limitations associated with the current oriented fiber scaffolds.
{"title":"Collagen-mediated in situ mineralization-enhanced biomimetic bone tissue engineering scaffolds","authors":"Rui Shi , Xiaotong Wang , Fangli Gang , Jiayu Shi , Shuping Wang , Wanting Liu , Weilong Ye , Xiaodan Sun","doi":"10.1016/j.colsurfb.2025.114566","DOIUrl":"10.1016/j.colsurfb.2025.114566","url":null,"abstract":"<div><div>Oriented poly(lactic acid) (PLA) fiber bone tissue engineering scaffolds are often limited by factors including poor material hydrophilicity and weak osteogenic activity. The introduction of in situ mineralization can address these issues, but it requires the assistance of hydrophilic materials to achieve optimal performance. Collagen, a nature-based ECM component, was adopted because it can enhance hydrophilicity, encourage cell adhesion, and biomimetrically induce mineralization, according to recent studies of ECM-mimicking scaffolds. Therefore, this study proposes a collagen-mediated in situ mineralization-enhanced scaffold design aimed at improving the hydrophilicity and osteogenic potential of oriented fiber scaffolds. Collagen (5–10 wt%) and phosphate-containing solutions (59.6 mM) were added to a PLA matrix, and scaffolds were electrospun at 12 kV. Subsequently, the scaffolds underwent in situ mineralization in a calcium ion-containing solution (101 mM), leading to the formation of calcium phosphate within the scaffold structure. The experimental results show that the introduction of collagen effectively promoted the formation of in situ mineralization, enhanced the hydrophilicity of the scaffold, and maintained good fiber orientation. The scaffolds exhibited significant mechanical anisotropy, with the Young's modulus parallel to the fiber direction reaching 5 MPa, which is 25 times greater than that in the direction perpendicular to the fibers. In vitro studies with rat bone marrow mesenchymal stem cells showed a 2.4-fold increase in osteogenic differentiation, as assessed by alkaline phosphatase activity. Micro-CT analysis showed that the increase of BV/TV was 3.26 times higher when compared to that of control scaffolds, while histological analysis revealed mature bone tissue formation characterized by well-organized collagen fibers. Overall, the present study describes a novel strategy of collagen-mediated in situ mineralization, first integrating enhanced hydrophilicity, mechanical anisotropy, and biomimetic bone-like properties to address major limitations associated with the current oriented fiber scaffolds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114566"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Approximately 5–10 % of fractures are associated with non-union, posing a significant challenge in orthopedic applications. Addressing this issue, innovative approaches beyond traditional grafting techniques like bone tissue engineering (BTE) are required. Biomaterials, combined with cells and bioactive molecules in BTE, are critical in managing non-union. Alginate, a natural polysaccharide, has gained widespread recognition in bone regeneration due to its bioavailability, its ability to form gels through crosslinking with divalent cations, and its cost-effectiveness. However, its inherent mechanical weaknesses necessitate a combinatorial approach with other biomaterials. In recent years, nanoscale biomaterials have gained prominence for bone regeneration due to their structural and compositional resemblance to natural bone, offering a supportive environment that regulates cell proliferation and differentiation for new bone formation. In this review, we briefly outline the synthesis of alginate-based nanocomposites using different fabrication techniques, such as hydrogels, 3D-printed scaffolds, fibers, and surface coatings with polymer, ceramic, carbon, metal, or lipid-based nanoparticles. These alginate-based nanocomposites elicit angiogenic, antibacterial, and immunomodulatory properties, thereby enhancing the osteogenic potential as an insightful measure for treating non-union. Despite the existence of similar literature, this work delivers a recent and focused examination of the latest advancements and insights on the potential of alginate-based nanocomposites for BTE applications. This review also underscores the obstacles that alginate-based nanocomposites must overcome to successfully transition into clinical applications.
{"title":"Recent progress in alginate-based nanocomposites for bone tissue engineering applications","authors":"Sundaravadhanan Lekhavadhani, Sushma Babu, Abinaya Shanmugavadivu, Nagarajan Selvamurugan","doi":"10.1016/j.colsurfb.2025.114570","DOIUrl":"10.1016/j.colsurfb.2025.114570","url":null,"abstract":"<div><div>Approximately 5–10 % of fractures are associated with non-union, posing a significant challenge in orthopedic applications. Addressing this issue, innovative approaches beyond traditional grafting techniques like bone tissue engineering (BTE) are required. Biomaterials, combined with cells and bioactive molecules in BTE, are critical in managing non-union. Alginate, a natural polysaccharide, has gained widespread recognition in bone regeneration due to its bioavailability, its ability to form gels through crosslinking with divalent cations, and its cost-effectiveness. However, its inherent mechanical weaknesses necessitate a combinatorial approach with other biomaterials. In recent years, nanoscale biomaterials have gained prominence for bone regeneration due to their structural and compositional resemblance to natural bone, offering a supportive environment that regulates cell proliferation and differentiation for new bone formation. In this review, we briefly outline the synthesis of alginate-based nanocomposites using different fabrication techniques, such as hydrogels, 3D-printed scaffolds, fibers, and surface coatings with polymer, ceramic, carbon, metal, or lipid-based nanoparticles. These alginate-based nanocomposites elicit angiogenic, antibacterial, and immunomodulatory properties, thereby enhancing the osteogenic potential as an insightful measure for treating non-union. Despite the existence of similar literature, this work delivers a recent and focused examination of the latest advancements and insights on the potential of alginate-based nanocomposites for BTE applications. This review also underscores the obstacles that alginate-based nanocomposites must overcome to successfully transition into clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114570"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-14DOI: 10.1016/j.colsurfb.2025.114564
Xi Peng , Yushi Wei , Yijing Liao , Xing Hu , Deming Gong , Guowen Zhang
The study aimed to investigate the inhibitory effect of hesperetin-copper (II) [Hsp-Cu(II)] on α-glucosidase in the presence of polysaccharides (xylan, β-glucan, low-, medium- and high-viscosity chitosan). The results showed that all the polysaccharides significantly reduced the inhibitory activity of α-glucosidase by Hsp-Cu(II), and the reduction effect of high-viscosity chitosan was the most significant. The polysaccharides significantly decreased the binding constant of Hsp-Cu(II)α-glucosidase, changed the binding sites of Hsp-Cu(II) to α-glucosidase and reduced the hydrogen bonds of Hsp-Cu(II) bound with α-glucosidase. Circular dichroism showed that the reduction of α-helix content in α-glucosidase caused by Hsp-Cu(II) was raised from 27.2 % to 29.5 %, 31.3 % and 32.7 % in the presence of xylan, β-glucan and high-viscosity chitosan, respectively, suggesting that the polysaccharides could restore the secondary structure of α-glucosidase. Fourier transforms infrared spectra showed that xylan and β-glucan formed hydrogen bonds with Hsp-Cu(II). The mechanism of the decreasing effect might be that the polysaccharides with the low viscosity compete with α-glucosidase to bind Hsp-Cu(II) through hydrogen bonds, restoring the catalytic center and active amino acid residues of Hsp-Cu(II) bound with α-glucosidase and the adsorption of high-viscosity chitosan decreases the binding affinity of Hsp-Cu(II) on α-glucosidase. The study may offer a reference for the development of Hsp-Cu(II)-based nutritional and healthy food for patients with hyperglycemia.
{"title":"Effect of polysaccharides on the inhibition and binding ability of hesperetin-copper(II) complex on α-glucosidase","authors":"Xi Peng , Yushi Wei , Yijing Liao , Xing Hu , Deming Gong , Guowen Zhang","doi":"10.1016/j.colsurfb.2025.114564","DOIUrl":"10.1016/j.colsurfb.2025.114564","url":null,"abstract":"<div><div>The study aimed to investigate the inhibitory effect of hesperetin-copper (II) [Hsp-Cu(II)] on α-glucosidase in the presence of polysaccharides (xylan, β-glucan, low-, medium- and high-viscosity chitosan). The results showed that all the polysaccharides significantly reduced the inhibitory activity of α-glucosidase by Hsp-Cu(II), and the reduction effect of high-viscosity chitosan was the most significant. The polysaccharides significantly decreased the binding constant of Hsp-Cu(II)<img>α-glucosidase, changed the binding sites of Hsp-Cu(II) to α-glucosidase and reduced the hydrogen bonds of Hsp-Cu(II) bound with α-glucosidase. Circular dichroism showed that the reduction of α-helix content in α-glucosidase caused by Hsp-Cu(II) was raised from 27.2 % to 29.5 %, 31.3 % and 32.7 % in the presence of xylan, β-glucan and high-viscosity chitosan, respectively, suggesting that the polysaccharides could restore the secondary structure of α-glucosidase. Fourier transforms infrared spectra showed that xylan and β-glucan formed hydrogen bonds with Hsp-Cu(II). The mechanism of the decreasing effect might be that the polysaccharides with the low viscosity compete with α-glucosidase to bind Hsp-Cu(II) through hydrogen bonds, restoring the catalytic center and active amino acid residues of Hsp-Cu(II) bound with α-glucosidase and the adsorption of high-viscosity chitosan decreases the binding affinity of Hsp-Cu(II) on α-glucosidase. The study may offer a reference for the development of Hsp-Cu(II)-based nutritional and healthy food for patients with hyperglycemia.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114564"},"PeriodicalIF":5.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.colsurfb.2025.114556
Jennifer Mariana Vargas López , José Alfonso Cruz Ramos , Gregorio Guadalupe Carbajal Arizaga
Unlike traditional materials designed to form large structures, many modern materials are presented in the form of powders resulting from a molecular level control of their composition and structure, making possible the miniaturization and fine-tuning of their properties to act in cellular dimensions with customized tasks. Several new materials for biomedical and microbiology applications appear every year. Although many of them are called nanomaterials, there may be a more precise description or classification. In this work, we review and detail the structural classification of nanometric, functionalized, hybrid and composite materials, mainly based on descriptions given by the International Union of Pure and Applied Chemistry (IUPAC). Besides we included smart and multifunctional materials, cassification based on performance. The second section shows how these materials are used in the area of medical microbiology, grouping these applications into barriers for microorganisms on surfaces, disinfectants in clinical practice, targeting of pathogens, detectors of microorganisms or their metabolites, and also as substrates to stabilize, transport, or nourish beneficial microorganisms. Finally, we will discuss some evidence that indicates the environmental risk and bacterial resistance alerts that should be taken into account with the use of these advanced powder materials.
{"title":"Revisiting the characteristics of nanomaterials, composites, hybrid and functionalized materials in medical microbiology","authors":"Jennifer Mariana Vargas López , José Alfonso Cruz Ramos , Gregorio Guadalupe Carbajal Arizaga","doi":"10.1016/j.colsurfb.2025.114556","DOIUrl":"10.1016/j.colsurfb.2025.114556","url":null,"abstract":"<div><div>Unlike traditional materials designed to form large structures, many modern materials are presented in the form of powders resulting from a molecular level control of their composition and structure, making possible the miniaturization and fine-tuning of their properties to act in cellular dimensions with customized tasks. Several new materials for biomedical and microbiology applications appear every year. Although many of them are called nanomaterials, there may be a more precise description or classification. In this work, we review and detail the structural classification of nanometric, functionalized, hybrid and composite materials, mainly based on descriptions given by the International Union of Pure and Applied Chemistry (IUPAC). Besides we included smart and multifunctional materials, cassification based on performance. The second section shows how these materials are used in the area of medical microbiology, grouping these applications into barriers for microorganisms on surfaces, disinfectants in clinical practice, targeting of pathogens, detectors of microorganisms or their metabolites, and also as substrates to stabilize, transport, or nourish beneficial microorganisms. Finally, we will discuss some evidence that indicates the environmental risk and bacterial resistance alerts that should be taken into account with the use of these advanced powder materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114556"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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