Pub Date : 2024-10-03DOI: 10.1016/j.colsurfb.2024.114292
Aliakbar Ebrahimi , Hamed Ghorbanpoor , Elif Apaydın , Bahar Demir Cevizlidere , Ceren Özel , Emre Tüfekçioğlu , Yücel Koç , Ahmet Emin Topal , Özlem Tomsuk , Kadri Güleç , Nuran Abdullayeva , Murat Kaya , Aynaz Ghorbani , Tayfun Şengel , Zineb Benzait , Onur Uysal , Ayla Eker Sarıboyacı , Fatma Doğan Güzel , Hemant Singh , Shabir Hassan , Huseyin Avci
Liver is responsible for the metabolization processes of up to 90 % of compounds and toxins in the body. Therefore liver-on-a-chip systems, as an in vitro promising cell culture platform, have great importance for fundamental science and drug development. In most of the liver-on-a-chip studies, seeding cells on both sides of a porous membrane, which represents the basement membrane, fail to resemble the native characteristics of biochemical, biophysical, and mechanical properties. In this study, polycarbonate (PC) and polyethylene terephthalate (PET) membranes were coated with gelatin to address this issue by accurately mimicking the native basement membrane present in the space of Disse. Various coating methods were used, including doctor blade, gel micro-injection, electrospinning, and spin coating. Spin coating was demonstrated to be the most effective technique owing to the ability to produce thin gel thickness with desirable surface roughness for cell interactions on both sides of the membrane. HepG2 and EA.HY926 cells were seeded on the upper and bottom sides of the gelatin-coated PET membrane and cultured on-chip for 7 days. Cell viability increased from 90 % to 95 %, while apoptotic index decreased. Albumin secretion notably rose between days 1–7 and 4–7, while GST-α secretion decreased from day 1 to day 7. In conclusion, the optimized spin coating process reported here can effectively modify the membranes to better mimic the native basement membrane niche characteristics.
{"title":"Convenient rapid prototyping microphysiological niche for mimicking liver native basement membrane: Liver sinusoid on a chip","authors":"Aliakbar Ebrahimi , Hamed Ghorbanpoor , Elif Apaydın , Bahar Demir Cevizlidere , Ceren Özel , Emre Tüfekçioğlu , Yücel Koç , Ahmet Emin Topal , Özlem Tomsuk , Kadri Güleç , Nuran Abdullayeva , Murat Kaya , Aynaz Ghorbani , Tayfun Şengel , Zineb Benzait , Onur Uysal , Ayla Eker Sarıboyacı , Fatma Doğan Güzel , Hemant Singh , Shabir Hassan , Huseyin Avci","doi":"10.1016/j.colsurfb.2024.114292","DOIUrl":"10.1016/j.colsurfb.2024.114292","url":null,"abstract":"<div><div>Liver is responsible for the metabolization processes of up to 90 % of compounds and toxins in the body. Therefore liver-on-a-chip systems, as an <em>in vitro</em> promising cell culture platform, have great importance for fundamental science and drug development. In most of the liver-on-a-chip studies, seeding cells on both sides of a porous membrane, which represents the basement membrane, fail to resemble the native characteristics of biochemical, biophysical, and mechanical properties. In this study, polycarbonate (PC) and polyethylene terephthalate (PET) membranes were coated with gelatin to address this issue by accurately mimicking the native basement membrane present in the space of Disse. Various coating methods were used, including doctor blade, gel micro-injection, electrospinning, and spin coating. Spin coating was demonstrated to be the most effective technique owing to the ability to produce thin gel thickness with desirable surface roughness for cell interactions on both sides of the membrane. HepG2 and EA.HY926 cells were seeded on the upper and bottom sides of the gelatin-coated PET membrane and cultured on-chip for 7 days. Cell viability increased from 90 % to 95 %, while apoptotic index decreased. Albumin secretion notably rose between days 1–7 and 4–7, while GST-α secretion decreased from day 1 to day 7. In conclusion, the optimized spin coating process reported here can effectively modify the membranes to better mimic the native basement membrane niche characteristics.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114292"},"PeriodicalIF":5.4,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.colsurfb.2024.114290
Qing Liu , Ye Zhang , Shuxian Yu , Chuanze Zhao , Yuqing Yang , Jianyu Yan , Yuge Wang , Dayong Liu , Ying Liu , Xu Zhang
Proteoglycans (PG) is crucial for regulating collagen formation and mineralization during bone tissue development. A wide variety of PG-modified collagen scaffolds have been proposed for bone engineering application to promote biological responses and work as artificial matrices that guide tissue regeneration. However, poor performance of theses biomaterials against infections has led to an unmet need for clinical prevention. Therefore, we utilized proanthocyanidins (PA) to simulate the functions of PG, including mediating the collagen assembly and intrafibrillar mineralization, to optimize scaffolds performance. The excellent antibacterial properties of PA can endow the scaffolds with anti-infection effects in the process of tissue regeneration. When PA was added during fibrillogenesis, the collagen fibrils appeared irregular aggregation and the mineralization degree was reduced. In contrast, the addition of PA after collagen self-assembly improved the latter’s ability to act as a deposition template and remarkably promoted mineral ions infiltration, thus enhancing intrafibrillar mineralization. The PA-modified scaffold displayed a highly hydrophilicity behaviour and long-term resistance to degradation. The sustained release of PA effectively inhibited the activity of Staphylococcus aureus. The scaffold also showed excellent biocompatibility and improved bone regeneration in calvarial critical-size defect models. The application of PA enables a dual-function scaffold with favourable intrafibrillar mineralization and anti-bacterial properties for bone regeneration.
{"title":"Proanthocyanidins modification of the mineralized collagen scaffold based on synchronous self-assembly/mineralization for bone regeneration","authors":"Qing Liu , Ye Zhang , Shuxian Yu , Chuanze Zhao , Yuqing Yang , Jianyu Yan , Yuge Wang , Dayong Liu , Ying Liu , Xu Zhang","doi":"10.1016/j.colsurfb.2024.114290","DOIUrl":"10.1016/j.colsurfb.2024.114290","url":null,"abstract":"<div><div>Proteoglycans (PG) is crucial for regulating collagen formation and mineralization during bone tissue development. A wide variety of PG-modified collagen scaffolds have been proposed for bone engineering application to promote biological responses and work as artificial matrices that guide tissue regeneration. However, poor performance of theses biomaterials against infections has led to an unmet need for clinical prevention. Therefore, we utilized proanthocyanidins (PA) to simulate the functions of PG, including mediating the collagen assembly and intrafibrillar mineralization, to optimize scaffolds performance. The excellent antibacterial properties of PA can endow the scaffolds with anti-infection effects in the process of tissue regeneration. When PA was added during fibrillogenesis, the collagen fibrils appeared irregular aggregation and the mineralization degree was reduced. In contrast, the addition of PA after collagen self-assembly improved the latter’s ability to act as a deposition template and remarkably promoted mineral ions infiltration, thus enhancing intrafibrillar mineralization. The PA-modified scaffold displayed a highly hydrophilicity behaviour and long-term resistance to degradation. The sustained release of PA effectively inhibited the activity of <em>Staphylococcus aureus.</em> The scaffold also showed excellent biocompatibility and improved bone regeneration in calvarial critical-size defect models. The application of PA enables a dual-function scaffold with favourable intrafibrillar mineralization and anti-bacterial properties for bone regeneration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114290"},"PeriodicalIF":5.4,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.colsurfb.2024.114284
Thao P. Doan-Nguyen , Anuwat Aunkham , Patitta Preedanorawut , Thanida Chanpong , Albert Schulte , Daniel Crespy , Wipa Suginta
We report the synthesis of functionalized nanodots as potential powerful blockers of solute transport through a chitoporin. Ultrasmall silica nanocapsules with a diameter of ∼ 6 nm were coated with chitooligosaccharides to be used as a “lid” binding to the opening of the chitoporin VhChiP of Vibrio campbellii. Efficient blocking is attributed to the adequate size of the nanodots and their functionalization with oligochitosan, which has strong affinity towards the Vibrio chitoporin. This strategy paves the way towards the development of nanomaterials for blocking other porins.
{"title":"Nanodots functionalized with chitooligosaccharides for blocking chitoporins","authors":"Thao P. Doan-Nguyen , Anuwat Aunkham , Patitta Preedanorawut , Thanida Chanpong , Albert Schulte , Daniel Crespy , Wipa Suginta","doi":"10.1016/j.colsurfb.2024.114284","DOIUrl":"10.1016/j.colsurfb.2024.114284","url":null,"abstract":"<div><div>We report the synthesis of functionalized nanodots as potential powerful blockers of solute transport through a chitoporin. Ultrasmall silica nanocapsules with a diameter of ∼ 6 nm were coated with chitooligosaccharides to be used as a “lid” binding to the opening of the chitoporin <em>Vh</em>ChiP of <em>Vibrio campbellii</em>. Efficient blocking is attributed to the adequate size of the nanodots and their functionalization with oligochitosan, which has strong affinity towards the <em>Vibrio</em> chitoporin. This strategy paves the way towards the development of nanomaterials for blocking other porins.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114284"},"PeriodicalIF":5.4,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.colsurfb.2024.114289
Xiangyang Ma , Liya Wang , Jingyu Chen , Enping Guo , Hongchen Zheng , Lei Zhao , Fuping Lu , Yihan Liu
Alkali proteases are crucial in numerous industries, especially in the laundry industry, but their inactivation by surfactants limits their effectiveness. This study employed substrate access tunnel engineering to improve the performance of WT bcPRO in surfactants. By modifying the key residues in the substrate pocket, the best variant N212S showed higher stability and activity in both AES and LAS. Molecular dynamics (MD) simulations provided insights into the enhanced stability and activity. The Asn212Ser mutation weakened the anti-correlation motion, increased the number of hydrogen bonds between amino acid residues, and made the protein structure more compact, contributing to its stability. Additionally, the mutation extended the substrate access tunnel and enabled additional interactions with the substrate, enhancing its catalytic activity in surfactants. This study demonstrates a strategy for reshaping the substrate access tunnel to improve protease stability and activity in surfactant environments, offering a promising protease candidate for the laundry industry.
{"title":"Surfactant-tolerance evolution of Bacillus clausii protease for enhancing activity and stability by reshaping the substrate access tunnel","authors":"Xiangyang Ma , Liya Wang , Jingyu Chen , Enping Guo , Hongchen Zheng , Lei Zhao , Fuping Lu , Yihan Liu","doi":"10.1016/j.colsurfb.2024.114289","DOIUrl":"10.1016/j.colsurfb.2024.114289","url":null,"abstract":"<div><div>Alkali proteases are crucial in numerous industries, especially in the laundry industry, but their inactivation by surfactants limits their effectiveness. This study employed substrate access tunnel engineering to improve the performance of WT bcPRO in surfactants. By modifying the key residues in the substrate pocket, the best variant N212S showed higher stability and activity in both AES and LAS. Molecular dynamics (MD) simulations provided insights into the enhanced stability and activity. The Asn212Ser mutation weakened the anti-correlation motion, increased the number of hydrogen bonds between amino acid residues, and made the protein structure more compact, contributing to its stability. Additionally, the mutation extended the substrate access tunnel and enabled additional interactions with the substrate, enhancing its catalytic activity in surfactants. This study demonstrates a strategy for reshaping the substrate access tunnel to improve protease stability and activity in surfactant environments, offering a promising protease candidate for the laundry industry.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114289"},"PeriodicalIF":5.4,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.colsurfb.2024.114291
Diego A. Bravo-Alfaro , Laura R. Ochoa-Rodríguez , Yevgen Prokhorov , Juan Francisco Pérez-Robles , Jessica M. Sampieri-Moran , Perla Elvia García-Casillas , Sujay Paul , Hugo S. García , Gabriel Luna-Bárcenas
Betulinic acid (BA) is a natural compound with significant potential for treating various diseases, including cancer and AIDS, and possesses additional anti-inflammatory and antibacterial properties. However, its clinical application is limited because of its low solubility in water, which impairs its distribution within the body. To overcome this challenge, nanoemulsions have been developed to improve the bioavailability of such poorly soluble drugs. This study investigated modified phosphatidylcholine (PC), where some fatty acids were replaced with conjugated linoleic acid (CLA) to stabilize BA nanoemulsions. The modified PC was used to prepare nanoemulsions with droplet sizes of up to 45 nanometers. These nanoemulsions maintained stability for 60 days at room temperature (25°C±2°C) and under refrigeration (5°C±1°C), with no signs of instability. Nanoemulsions stabilized with CLA-modified PC achieved a higher drug encapsulation rate (93.5±4.3 %) than those using natural PC (82.8±4.2 %). In an in vivo model, both nanoemulsion formulations significantly increased BA absorption, with CLA-modified PC enhancing absorption by 21.3±1.3 times and natural PC by 20±2.3 times compared to the free drug. This suggests that nanoemulsions with modified PC could improve the stability and efficacy of BA in clinical applications.
白桦脂酸（BA）是一种天然化合物，具有治疗癌症和艾滋病等多种疾病的巨大潜力，并具有额外的消炎和抗菌特性。然而，由于白桦脂酸在水中的溶解度较低，影响了其在体内的分布，因此其临床应用受到了限制。为了克服这一难题，人们开发了纳米乳剂来提高这类溶解性较差药物的生物利用度。本研究对改性磷脂酰胆碱（PC）进行了研究，用共轭亚油酸（CLA）取代了部分脂肪酸，以稳定 BA 纳米乳剂。改性 PC 被用于制备液滴大小达 45 纳米的纳米乳剂。这些纳米乳剂在室温（25°C±2°C）和冷藏（5°C±1°C）条件下可保持稳定 60 天，且无不稳定性迹象。与使用天然 PC 的纳米乳剂（82.8±4.2%）相比，使用 CLA 改性 PC 稳定的纳米乳剂实现了更高的药物包封率（93.5±4.3%）。在体内模型中，两种纳米乳剂配方都能显著提高 BA 的吸收率，与游离药物相比，CLA 改性 PC 的吸收率提高了 21.3±1.3 倍，天然 PC 的吸收率提高了 20±2.3 倍。这表明改性 PC 纳米乳剂可提高 BA 在临床应用中的稳定性和疗效。
{"title":"Nanoemulsions of betulinic acid stabilized with modified phosphatidylcholine increase the stability of the nanosystems and the drug's bioavailability","authors":"Diego A. Bravo-Alfaro , Laura R. Ochoa-Rodríguez , Yevgen Prokhorov , Juan Francisco Pérez-Robles , Jessica M. Sampieri-Moran , Perla Elvia García-Casillas , Sujay Paul , Hugo S. García , Gabriel Luna-Bárcenas","doi":"10.1016/j.colsurfb.2024.114291","DOIUrl":"10.1016/j.colsurfb.2024.114291","url":null,"abstract":"<div><div>Betulinic acid (BA) is a natural compound with significant potential for treating various diseases, including cancer and AIDS, and possesses additional anti-inflammatory and antibacterial properties. However, its clinical application is limited because of its low solubility in water, which impairs its distribution within the body. To overcome this challenge, nanoemulsions have been developed to improve the bioavailability of such poorly soluble drugs. This study investigated modified phosphatidylcholine (PC), where some fatty acids were replaced with conjugated linoleic acid (CLA) to stabilize BA nanoemulsions. The modified PC was used to prepare nanoemulsions with droplet sizes of up to 45 nanometers. These nanoemulsions maintained stability for 60 days at room temperature (25°C±2°C) and under refrigeration (5°C±1°C), with no signs of instability. Nanoemulsions stabilized with CLA-modified PC achieved a higher drug encapsulation rate (93.5±4.3 %) than those using natural PC (82.8±4.2 %). In an <em>in vivo</em> model, both nanoemulsion formulations significantly increased BA absorption, with CLA-modified PC enhancing absorption by 21.3±1.3 times and natural PC by 20±2.3 times compared to the free drug. This suggests that nanoemulsions with modified PC could improve the stability and efficacy of BA in clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114291"},"PeriodicalIF":5.4,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.colsurfb.2024.114287
Zhimin Mo , Yuxuan Luo , Qi Xu , Jiexi Liang , Zimeng Wang , Qianyuan He , Zushun Xu
The acidic nature of malignant tumors leads to increased drug sequestration and the evasion of apoptotic damage, which is further exacerbated by abnormal lysosomes in tumor cells. In this study, a "lysosomal bomb" will be constructed using a type of acid-neutralized amorphous calcium carbonate (ACC) to encapsulate the sonosensitizer protoporphyrin IX (PpIX), and then coated with homologous tumor cell membranes to increase water solubility and homologous targeting. The PpIX-ACC@CMs designed in this paper are popcorn-like structures, which can not only neutralize the tumor's acidic microenvironment to balance the pH value and release excess Ca2+, but also cause lysosomal dysfunction and achieve drug lysosomal escape to increase drug accumulation. Additionally, the CO2 gas nucleus produced by the acid reaction of ACC can increase the ultrasonic cavitation effect to amplify the sonodynamic therapy (SDT) effect. In vitro and in vivo experiments demonstrated that PpIX-ACC@CMs, serving as a "lysosomal bomb," successfully localized to lysosomes of tumor cells and exhibited lysosomal escape ability through its acid reaction ability, achieving excellent SDT efficacy under ultrasound stimulation. Furthermore, exogenous Ca2+ overload also increased the likelihood of tumor calcification, which could contribute to in vivo tumor inhibition and facilitate CT medical imaging to monitor treatment efficacy.
{"title":"A \"lysosomal bomb\" constructed based on amorphous calcium carbonate to induce tumor apoptosis by amplified sonodynamic therapy","authors":"Zhimin Mo , Yuxuan Luo , Qi Xu , Jiexi Liang , Zimeng Wang , Qianyuan He , Zushun Xu","doi":"10.1016/j.colsurfb.2024.114287","DOIUrl":"10.1016/j.colsurfb.2024.114287","url":null,"abstract":"<div><div>The acidic nature of malignant tumors leads to increased drug sequestration and the evasion of apoptotic damage, which is further exacerbated by abnormal lysosomes in tumor cells. In this study, a \"lysosomal bomb\" will be constructed using a type of acid-neutralized amorphous calcium carbonate (ACC) to encapsulate the sonosensitizer protoporphyrin IX (PpIX), and then coated with homologous tumor cell membranes to increase water solubility and homologous targeting. The PpIX-ACC@CMs designed in this paper are popcorn-like structures, which can not only neutralize the tumor's acidic microenvironment to balance the pH value and release excess Ca<sup>2+</sup>, but also cause lysosomal dysfunction and achieve drug lysosomal escape to increase drug accumulation. Additionally, the CO<sub>2</sub> gas nucleus produced by the acid reaction of ACC can increase the ultrasonic cavitation effect to amplify the sonodynamic therapy (SDT) effect. <em>In vitro</em> and <em>in vivo</em> experiments demonstrated that PpIX-ACC@CMs, serving as a \"lysosomal bomb,\" successfully localized to lysosomes of tumor cells and exhibited lysosomal escape ability through its acid reaction ability, achieving excellent SDT efficacy under ultrasound stimulation. Furthermore, exogenous Ca<sup>2+</sup> overload also increased the likelihood of tumor calcification, which could contribute to <em>in vivo</em> tumor inhibition and facilitate CT medical imaging to monitor treatment efficacy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114287"},"PeriodicalIF":5.4,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.colsurfb.2024.114279
Guang-Cong Zhang , Kang Song , Xiao-Fan Wang , Zongyan He , Jun Du , Jia-Lei Sun , Ru-Chen Xu , Zhi-Yong Liu , Fu Wang , Zhuo-Ran Qi , Xiang-Nan Yu , Yuqing Miao , Ling Dong , Shu-Qiang Weng , Xi-Zhong Shen , Tao-Tao Liu , Yuhao Li , Ji-Min Zhu
Sorafenib (SOR), a multi-kinase inhibitor for advanced hepatocellular carcinoma (HCC), has limited clinical application due to severe side effects and drug resistance. To overcome these challenges, we developed a bismuth-based nanomaterial (BOS) for thermal injury-assisted continuous targeted therapy in HCC. Initially, the mesoporous nanomaterial was loaded with SOR, forming the BOS@SOR nano-carrier system for drug delivery and controlled release. Notably, compared to targeted or photothermal therapy alone, the combination therapy using this nano-carrier system significantly impaired cell proliferation and increased apoptosis. In vivo efficacy evaluations demonstrated that BOS@SOR exhibited excellent biocompatibility, confirmed through hemolysis and biochemical analyses. Additionally, BOS@SOR enhanced contrast in computed tomography, aiding in the precise identification of HCC size and location. The photothermal therapeutic properties of bismuth further contributed to the synergistic anti-tumor activity of BOS@SOR, significantly reducing tumor growth in an orthotopic xenograft HCC model. Taken together, encapsulating SOR within a bismuth-based mesoporous nanomaterial creates a multifunctional and environmentally stable nanocomposite (BOS@SOR), enhancing the therapeutic effect of SOR and presenting an effective strategy for HCC treatment.
索拉非尼（SOR）是一种治疗晚期肝细胞癌（HCC）的多激酶抑制剂，但由于其严重的副作用和耐药性，其临床应用十分有限。为了克服这些挑战，我们开发了一种铋基纳米材料（BOS），用于热损伤辅助的 HCC 连续靶向治疗。首先，在介孔纳米材料中负载 SOR，形成 BOS@SOR 纳米载体系统，实现药物的输送和控释。值得注意的是，与单独的靶向治疗或光热治疗相比，利用这种纳米载体系统进行的联合治疗能显著抑制细胞增殖并增加细胞凋亡。体内疗效评估表明，BOS@SOR 具有良好的生物相容性，溶血和生化分析证实了这一点。此外，BOS@SOR 还增强了计算机断层扫描的对比度，有助于精确确定 HCC 的大小和位置。铋的光热治疗特性进一步促进了 BOS@SOR 的协同抗肿瘤活性，显著降低了正位异种移植 HCC 模型中肿瘤的生长。综上所述，将 SOR 包封在铋基介孔纳米材料中形成了一种多功能、环境稳定的纳米复合材料（BOS@SOR），增强了 SOR 的治疗效果，为 HCC 治疗提供了一种有效的策略。
{"title":"Bismuth-based mesoporous nanoball carrying sorafenib for synergistic photothermal and molecularly-targeted therapy in an orthotopic hepatocellular carcinoma xenograft mouse model","authors":"Guang-Cong Zhang , Kang Song , Xiao-Fan Wang , Zongyan He , Jun Du , Jia-Lei Sun , Ru-Chen Xu , Zhi-Yong Liu , Fu Wang , Zhuo-Ran Qi , Xiang-Nan Yu , Yuqing Miao , Ling Dong , Shu-Qiang Weng , Xi-Zhong Shen , Tao-Tao Liu , Yuhao Li , Ji-Min Zhu","doi":"10.1016/j.colsurfb.2024.114279","DOIUrl":"10.1016/j.colsurfb.2024.114279","url":null,"abstract":"<div><div>Sorafenib (SOR), a multi-kinase inhibitor for advanced hepatocellular carcinoma (HCC), has limited clinical application due to severe side effects and drug resistance. To overcome these challenges, we developed a bismuth-based nanomaterial (BOS) for thermal injury-assisted continuous targeted therapy in HCC. Initially, the mesoporous nanomaterial was loaded with SOR, forming the BOS@SOR nano-carrier system for drug delivery and controlled release. Notably, compared to targeted or photothermal therapy alone, the combination therapy using this nano-carrier system significantly impaired cell proliferation and increased apoptosis. <em>In vivo</em> efficacy evaluations demonstrated that BOS@SOR exhibited excellent biocompatibility, confirmed through hemolysis and biochemical analyses. Additionally, BOS@SOR enhanced contrast in computed tomography, aiding in the precise identification of HCC size and location. The photothermal therapeutic properties of bismuth further contributed to the synergistic anti-tumor activity of BOS@SOR, significantly reducing tumor growth in an orthotopic xenograft HCC model. Taken together, encapsulating SOR within a bismuth-based mesoporous nanomaterial creates a multifunctional and environmentally stable nanocomposite (BOS@SOR), enhancing the therapeutic effect of SOR and presenting an effective strategy for HCC treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114279"},"PeriodicalIF":5.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.colsurfb.2024.114286
Chen Liu , Wanchun Du , Liang Zhang , Jiacheng Wang
Curcumin (Cur) is a natural polyphenol that is one of the most valuable natural products. However, its use as a functional food is limited by low water solubility, chemical instability and poor bioavailability. In this study, a supramolecular co-assembly strategy was used to construct an oleanolic acid-curcumin (OLA-Cur) co-assembly composite nano-slow-release treatment system. As a co-assembled compound, OLA is a widely present pentacyclic triterpenoid compound with multiple biological activities in the plant kingdom, which is expected to jointly alleviate the damaging effects of papain-induced mouse osteoarthritis model. The OLA-Cur NPs shows the solid core-shell structure, which can effectively improve the water solubility of Cur and OLA, and has good stability and sustained release characteristics. The analysis results show that the two compounds are mainly assembled through hydrogen bonding interactions, hydrophobic interactions, and π - π stacking interactions. The OLA-Cur NPs can inhibit the release of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β induced by LPS in RAW264.7 mouse macrophages, promote the secretion of anti-inflammatory cytokine IL-10, and improve the oxidative stress index of hydrogen peroxide induced human rheumatoid arthritis synovial fibroblasts. In addition, it has a certain improvement effect on cartilage and subchondral bone damage in mouse osteoarthritis models. These findings suggest that constructing co-assembled composite nanoparticles based on pure natural compounds may break through the limitations of a variety of important nutritional ingredients in functional foods.
{"title":"Natural synergy: Oleanolic acid-curcumin co-assembled nanoparticles combat osteoarthritis","authors":"Chen Liu , Wanchun Du , Liang Zhang , Jiacheng Wang","doi":"10.1016/j.colsurfb.2024.114286","DOIUrl":"10.1016/j.colsurfb.2024.114286","url":null,"abstract":"<div><div>Curcumin (Cur) is a natural polyphenol that is one of the most valuable natural products. However, its use as a functional food is limited by low water solubility, chemical instability and poor bioavailability. In this study, a supramolecular co-assembly strategy was used to construct an oleanolic acid-curcumin (OLA-Cur) co-assembly composite nano-slow-release treatment system. As a co-assembled compound, OLA is a widely present pentacyclic triterpenoid compound with multiple biological activities in the plant kingdom, which is expected to jointly alleviate the damaging effects of papain-induced mouse osteoarthritis model. The OLA-Cur NPs shows the solid core-shell structure, which can effectively improve the water solubility of Cur and OLA, and has good stability and sustained release characteristics. The analysis results show that the two compounds are mainly assembled through hydrogen bonding interactions, hydrophobic interactions, and π - π stacking interactions. The OLA-Cur NPs can inhibit the release of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β induced by LPS in RAW264.7 mouse macrophages, promote the secretion of anti-inflammatory cytokine IL-10, and improve the oxidative stress index of hydrogen peroxide induced human rheumatoid arthritis synovial fibroblasts. In addition, it has a certain improvement effect on cartilage and subchondral bone damage in mouse osteoarthritis models. These findings suggest that constructing co-assembled composite nanoparticles based on pure natural compounds may break through the limitations of a variety of important nutritional ingredients in functional foods.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114286"},"PeriodicalIF":5.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30DOI: 10.1016/j.colsurfb.2024.114278
Hagai Klein , Raz Cohen , Karthik Ananth Mani , Reut Amar Feldbaum , Avital Ben-Haim , Einat Zelinger , Narsingh R. Nirala , Divagar Muthukumar , Abraham J. Domb , Giorgi Shtenberg , Guy Mechrez
Pickering emulsions are excellent candidates for developing soft biosensors utilized for detecting native biomolecules such as peptides and proteins through the Surface-Enhanced Raman Spectroscopy (SERS) transduction mechanism. Here, we have developed a SERS sensor based on oil-in-water Pickering emulsions stabilized by Ag nanoparticles (Ag-NPs) with the Raman active molecule (4-Aminothiphenol, 4ATP) adsorbed to their surface. The structural properties and composition of the Pickering emulsion were tuned to meet the demands of the maximal optical response. Our results show that the obtained SERS signals of the main studied Pickering emulsion (water: oil ratio 7:3, 1 wt% Ag-NPs) outperformed colloidal dispersions with the same Ag-NPs concentration by 10-fold at any studied content of 4ATP. The superior optical response of the Pickering emulsion compared to the colloidal dispersion can thus pave the way for the detection of a large variety of analytes at high sensitivity by a soft sensing device. This study innovates by comparing the SERS signals of Raman-active Ag-NPs when they are assembled at the oil/water interface of an emulsion to the case where the NPs are individually dispersed in the medium. The findings shed light on the edit value of utilizing Raman-active Pickering stabilizers for biosensing applications.
{"title":"Soft surface-enhanced Raman scattering sensing platform based on an oil-in-water emulsion stabilized by silver nanoparticles","authors":"Hagai Klein , Raz Cohen , Karthik Ananth Mani , Reut Amar Feldbaum , Avital Ben-Haim , Einat Zelinger , Narsingh R. Nirala , Divagar Muthukumar , Abraham J. Domb , Giorgi Shtenberg , Guy Mechrez","doi":"10.1016/j.colsurfb.2024.114278","DOIUrl":"10.1016/j.colsurfb.2024.114278","url":null,"abstract":"<div><div>Pickering emulsions are excellent candidates for developing soft biosensors utilized for detecting native biomolecules such as peptides and proteins through the Surface-Enhanced Raman Spectroscopy (SERS) transduction mechanism. Here, we have developed a SERS sensor based on oil-in-water Pickering emulsions stabilized by Ag nanoparticles (Ag-NPs) with the Raman active molecule (4-Aminothiphenol, 4ATP) adsorbed to their surface. The structural properties and composition of the Pickering emulsion were tuned to meet the demands of the maximal optical response. Our results show that the obtained SERS signals of the main studied Pickering emulsion (water: oil ratio 7:3, 1 wt% Ag-NPs) outperformed colloidal dispersions with the same Ag-NPs concentration by 10-fold at any studied content of 4ATP. The superior optical response of the Pickering emulsion compared to the colloidal dispersion can thus pave the way for the detection of a large variety of analytes at high sensitivity by a soft sensing device. This study innovates by comparing the SERS signals of Raman-active Ag-NPs when they are assembled at the oil/water interface of an emulsion to the case where the NPs are individually dispersed in the medium. The findings shed light on the edit value of utilizing Raman-active Pickering stabilizers for biosensing applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114278"},"PeriodicalIF":5.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natural rubber originates from the coagulation of rubber particles (RP) from Hevea brasiliensis latex. The size distribution of Hevea RP is bimodal with the presence of small rubber particles (SRP) and large rubber particles (LRP). This study aims at getting a better understanding of the early coagulation steps of Hevea RP taking into account the particle size. SRP and LRP were obtained by centrifugation of freshly tapped ammonia-free latex from RRIM600 clone. Size and zeta potential measurements showed that both RP fractions were efficiently separated and stable in basic buffer. SRP and LRP dispersions were placed in a Langmuir trough and RP were let to adsorb at the air-liquid interface to form interfacial films. Surface tension and ellipsometry indicate that the formation kinetics and the stabilization of the film at the air-liquid interface are faster for SRP than LRP. Moreover, the arrangement of RP at the interface differs between SRP and LRP, as shown by Brewster angle microscopy, atomic force microscopy and confocal laser scanning microscopy. First, the RP membrane and cis-1,4-polyisoprene core spread at the air-liquid interface before clustering. Then, while the SRP fuse, the LRP keep their structure in individual particles in floating aggregate. The role of the non-isoprene molecules on the different organization of SRP and LRP films is discussed, the one of the two major RP proteins, SRPP1 (Small Rubber Particle Protein) and Rubber Elongation Factor (REF1) in the early coagulation steps.
天然橡胶源自巴西橡胶树胶乳的橡胶颗粒（RP）的凝结。Hevea RP 的尺寸分布呈双峰型，存在小橡胶颗粒（SRP）和大橡胶颗粒（LRP）。本研究旨在根据粒度更好地了解 Hevea RP 的早期凝结步骤。SRP 和 LRP 是通过离心分离 RRIM600 克隆的新鲜无氨胶乳获得的。粒度和 zeta 电位测量结果表明，两种胶乳馏分都能在碱性缓冲液中有效分离并保持稳定。将 SRP 和 LRP 分散液置于 Langmuir 槽中，让 RP 在空气-液体界面上吸附，形成界面薄膜。表面张力和椭偏仪表明，SRP 的形成动力学和薄膜在气液界面的稳定性比 LRP 快。此外，布儒斯特角显微镜、原子力显微镜和共聚焦激光扫描显微镜显示，SRP 和 LRP 在界面上的 RP 排列方式也有所不同。首先，RP 膜和顺式-1,4-聚异戊二烯核心在气液界面上扩散，然后聚成一团。然后，在 SRP 融合的同时，LRP 在浮动聚合体中保持其单个颗粒的结构。讨论了非异戊二烯分子对 SRP 和 LRP 薄膜不同组织结构的作用，以及两种主要 RP 蛋白 SRPP1（小橡胶颗粒蛋白）和橡胶伸长因子 (REF1) 在早期凝结步骤中的作用。
{"title":"Hevea brasiliensis rubber particles' fluid interfaces reveal size impact on early coagulation steps","authors":"Marion Baudoin , Gilles Paboeuf , Siriluck Liengprayoon , Natedao Musigamart , Céline Bottier , Véronique Vié","doi":"10.1016/j.colsurfb.2024.114281","DOIUrl":"10.1016/j.colsurfb.2024.114281","url":null,"abstract":"<div><div>Natural rubber originates from the coagulation of rubber particles (RP) from <em>Hevea brasiliensis</em> latex. The size distribution of <em>Hevea</em> RP is bimodal with the presence of small rubber particles (SRP) and large rubber particles (LRP). This study aims at getting a better understanding of the early coagulation steps of <em>Hevea</em> RP taking into account the particle size. SRP and LRP were obtained by centrifugation of freshly tapped ammonia-free latex from RRIM600 clone. Size and zeta potential measurements showed that both RP fractions were efficiently separated and stable in basic buffer. SRP and LRP dispersions were placed in a Langmuir trough and RP were let to adsorb at the air-liquid interface to form interfacial films. Surface tension and ellipsometry indicate that the formation kinetics and the stabilization of the film at the air-liquid interface are faster for SRP than LRP. Moreover, the arrangement of RP at the interface differs between SRP and LRP, as shown by Brewster angle microscopy, atomic force microscopy and confocal laser scanning microscopy. First, the RP membrane and <em>cis</em>-1,4-polyisoprene core spread at the air-liquid interface before clustering. Then, while the SRP fuse, the LRP keep their structure in individual particles in floating aggregate. The role of the non-isoprene molecules on the different organization of SRP and LRP films is discussed, the one of the two major RP proteins, SRPP1 (Small Rubber Particle Protein) and Rubber Elongation Factor (REF1) in the early coagulation steps.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"Article 114281"},"PeriodicalIF":5.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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