Colloids and Surfaces B: Biointerfaces最新文献_第10页

Polyurethane in bone tissue engineering: 3D-Bioprinting Vs. electrospinning 骨组织工程中的聚氨酯：3d生物打印Vs.静电纺丝。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115270

Seyyed Ahmad Seyyed Nasrollah , Mobina Bazari , Nivad Ahmadian , Hamed Alizadeh Sardroud , Emad Naseri , Joel D. Bumgardner , Ali Sadeghianmaryan

Bone injuries caused by trauma or disease affect millions of people around the world. Although bone fixation, allographs, and autographs are established procedures for addressing bone injuries, their applications are subject to subsequent surgical removal, risk of disease transmission, or danger of morbidity associated with healing. To overcome these limits, bone tissue engineering employs a temporary matrix or scaffold to repair and restore damaged bone. Various biomaterials have been used for bone tissue engineering, and polyurethane has superior mechanical properties compared to other biomaterials. Electrospinning and bioprinting are gaining much interest in bone tissue engineering. These manufacturing methods can provide excellent control over the geometry and porosity of the scaffolds, which would give tunability in cell viability and growth. The review article examines recent publications on the use of 3D bioprinted and electrospun polyurethane scaffolds for bone tissue engineering and their advantages. Some of these advantages include creating optimal microenvironments for cell adhesion and proliferation, offering a wide range of sizes and mechanical properties that enable personalized scaffolds based on the size and location of bone injuries and defects, and the ability to incorporate osteoinductive and osteogenic agents into the scaffold structure. Also, this review identifies knowledge gaps, such as cell-loaded polyurethane bioinks due to their non-hydrogel structure or lack of 3D cell culture in electrospun scaffolds, and suggests future research directions in the field.

创伤或疾病造成的骨损伤影响着全世界数百万人。虽然骨固定、同种异体和签名是解决骨损伤的既定程序，但它们的应用受到后续手术切除、疾病传播风险或与愈合相关的发病率风险的影响。为了克服这些限制，骨组织工程采用临时基质或支架来修复和恢复受损的骨骼。各种生物材料已被用于骨组织工程，聚氨酯与其他生物材料相比具有优越的机械性能。静电纺丝和生物打印技术是骨组织工程研究的热点。这些制造方法可以很好地控制支架的几何形状和孔隙度，从而可以调节细胞的活力和生长。本文综述了最近发表的关于生物3D打印和静电纺聚氨酯支架在骨组织工程中的应用及其优势。这些优势包括为细胞粘附和增殖创造最佳的微环境，提供广泛的尺寸和机械性能，使基于骨损伤和缺陷的大小和位置的个性化支架成为可能，以及将骨诱导和成骨剂纳入支架结构的能力。此外，本文还指出了知识空白，例如细胞负载聚氨酯生物墨水由于其非水凝胶结构或在电纺丝支架中缺乏3D细胞培养，并提出了该领域未来的研究方向。

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引用次数: 0

Small molecule-stabilized exceedingly small magnetic iron oxide nanoparticles as a contrast agent of T1-weighted magnetic resonance imaging 小分子稳定的极小磁性氧化铁纳米颗粒作为t1加权磁共振成像的造影剂。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115268

Ruilong Zhou , Basheng Hu , Guochao Wu , Sihua Yan , Bin Ren , Zongheng Li , Qingdeng Fan , Jing Yang , Ya Huang , Jiaoyang Zhu , Jie Feng , Ge Wen , Guining Zhang , Shipeng Ning , Zhao Chen , Zheyu Shen

Exceedingly small magnetic iron oxide nanoparticles (ES-MIONs) with high biocompatibility have been developed as T₁-weighted magnetic resonance imaging (MRI) contrast agents (CAs) with potential to replace commercial Gd chelates. To overcome the key problem of clinical transformation (high viscosity at high concentration) for reported ES-MIONs stabilized by polymers, herein, we propose a new concept to synthesize ES-MIONs stabilized by small molecules, but not polymers. Typically, sodium citrate (SC) was used as the stabilizer to synthesize ES-MION-SC through a co-precipitation method. After optimization of large-scale synthesis conditions, ES-MION-SC24 was considered as the optimal sample because of its high r₁ value (7.03 ± 0.05 mM⁻¹ s⁻¹, 3.0 T). At 100 mM of C_Fe, the viscosity of ES-MION-SC24 (only 1.0 CP) is much lower than that of ES-MION stabilized by polyacrylic acid (PAA) (∼11.0 CP), which exhibits promising potential for clinical application of ES-MION-SC24. The MTT assay, live/dead cells assay, blood routine analysis, and histological analysis show ES-MION-SC24 exhibits favorable biosafety. T₁-weighted MRI at 3.0 T indicates ES-MION-SC24 can accumulate in tumors and maintain high brightness for an extended period up to 2.0 h enabling multiple scans post-injection, which is much longer than commercial MRI CAs.

具有高生物相容性的极小磁性氧化铁纳米颗粒（ES-MIONs）已被开发为t1加权磁共振成像（MRI）造影剂（CAs），具有取代商业Gd螯合物的潜力。针对目前已报道的聚合物稳定ES-MIONs在临床转化中存在的高黏度问题，本文提出了一种合成非聚合物稳定ES-MIONs的新思路。通常采用柠檬酸钠（SC）作为稳定剂，通过共沉淀法合成es - ion -SC。经过大规模合成条件优化，ES-MION-SC24的r1值较高（7.03 ±0.05 mM-1 s-1, 3.0 T），被认为是最佳样品。在CFe为100 mM时，ES-MION- sc24的黏度（仅为1.0 CP）远低于聚丙烯酸（PAA）稳定的ES-MION- sc24的黏度（约11.0 CP），这表明ES-MION- sc24具有良好的临床应用潜力。MTT试验、活/死细胞试验、血常规分析和组织学分析表明es - ion - sc24具有良好的生物安全性。3.0 T的t1加权MRI表明es - ion - sc24可以在肿瘤中积累，并在长达2.0 h的延长时间内保持高亮度，从而可以在注射后进行多次扫描，这比商用MRI ca要长得多。

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引用次数: 0

Design and optimization of indomethacin-loaded solid dispersion orally dissolving films: Machine learning and molecular dynamics study 吲哚美辛固体分散口腔溶膜的设计与优化：机器学习与分子动力学研究。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115257

Jianlu Qu , Changhao Jia , Xiaoyang Zhang , Wenlong Li

Indomethacin (IND) is a nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic properties, and is widely used for the treatment of diverse inflammatory diseases. However, its poor aqueous solubility severely limits its clinical application. An orally dissolving film (ODF) loaded with an indomethacin solid dispersion (IND-SD) was developed to enhance dissolution. Based on Hansen solubility parameters (HSP), four polymers were selected to prepare the IND-SD, and the optimal carrier and drug to polymer ratio were identified by in vitro dissolution testing. Molecular docking and molecular dynamics (MD) simulations were employed to elucidate drug-polymer interactions at the molecular level. Under the guidance of Quality by Design (QbD), an optimization framework integrating a Box-Behnken design (BBD) and an artificial neural network (ANN) was established to design and optimize the ODF formulation. Multiple statistical metrics were used to assess the Box-Behnken design response surface methodology (BBD-RSM) model and the ANN model, with the ANN model demonstrating superior predictive accuracy in predicting the film critical quality attributes (CQAs). PXRD and DSC analyses confirmed that IND existed in an amorphous state in both the IND-SD and the ODF. In vitro dissolution experiments demonstrated that the cumulative drug release from the ODF in simulated saliva within 1 min was significantly higher than that of pure IND and IND-SD.

吲哚美辛（Indomethacin， IND）是一种具有抗炎、镇痛和解热作用的非甾体类抗炎药（NSAID），广泛用于多种炎症性疾病的治疗。但其水溶性差严重限制了其临床应用。研制了一种负载吲哚美辛固体分散体（IND-SD）的口腔溶出膜（ODF），以提高其溶出度。基于Hansen溶解度参数（HSP），选择4种聚合物制备IND-SD，并通过体外溶出度试验确定最佳载体和药聚合物比。分子对接和分子动力学（MD）模拟在分子水平上阐明了药物-聚合物相互作用。在设计质量（Quality by Design, QbD）的指导下，建立了Box-Behnken设计（BBD）和人工神经网络（ANN）相结合的优化框架，对ODF配方进行设计和优化。使用多个统计指标来评估Box-Behnken设计响应面方法（BBD-RSM）模型和人工神经网络模型，人工神经网络模型在预测薄膜关键质量属性（cqa）方面表现出更高的预测准确性。PXRD和DSC分析证实IND在IND- sd和ODF中均以非晶态存在。体外溶出实验表明，ODF在模拟唾液中1 min内的累积药物释放量明显高于纯IND和IND- sd。

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引用次数: 0

Engineered reconstructed extracellular vesicles coloaded with Astragaloside IV/Paeoniflorin improve cerebral ischemia/reperfusion injury by inhibiting the inflammatory response 黄芪甲苷/芍药苷复合重建的细胞外囊泡通过抑制炎症反应改善脑缺血再灌注损伤。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115267

Long Chen , Meiling Tang , Jiaxiang Zou , Jingyuan Mo , Yingli Gan , Xingli Wen , Pengcheng Wang , Zai Liu , Lisheng Wang

The method of nourishing qi and activating blood circulation is a key approach in traditional Chinese medicine for treating stroke. The combination of Astragaloside IV (qi-nourishing) and Paeoniflorin (blood-activating) in the BuYang HuanWu Decoction can enhance its therapeutic effects. Extracellular vesicles offer several advantages, including low immunogenicity, biodegradability, and the ability to cross the blood-brain barrier, making them ideal carriers for drug delivery. In this study, astragaloside IV and paeoniflorin were co-loaded into extracellular vesicles derived from milk using ultrasound, and RVG29 peptide was modified on their surface through hydrophobic insertion to achieve targeted delivery to the brain lesion area. Cell and small animal imaging experiments confirmed that these engineered reconstructed extracellular vesicles possess excellent brain-targeting capabilities. In the mouse model of cerebral artery occlusion/reperfusion, this co-delivery system significantly suppressed inflammation and improved brain injury, providing a new strategy for treating cerebral ischemia and demonstrating promising application prospects.

益气活血法是中医治疗中风的重要方法。补阳还五汤中补益益气的黄芪甲苷与活血的芍药苷合用可增强其治疗效果。细胞外囊泡具有多种优点，包括低免疫原性、可生物降解性和穿越血脑屏障的能力，使其成为药物输送的理想载体。本研究利用超声技术将黄芪甲苷和芍药苷共负载到乳源性细胞外囊泡中，并通过疏水插入对其表面的RVG29肽进行修饰，实现对脑病变区域的靶向递送。细胞和小动物成像实验证实，这些工程重建的细胞外囊泡具有出色的脑靶向能力。在小鼠脑动脉闭塞/再灌注模型中，该共给药系统显著抑制炎症反应，改善脑损伤，为治疗脑缺血提供了新的策略，具有良好的应用前景。

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引用次数: 0

Influence of the polycation structure on the physicochemical and biological properties of polyplex of heparin and aptamers systems 多阳离子结构对肝素及其适体多聚体理化生物学性质的影响

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115269

Małgorzata Tymecka , Magdalena Skóra , Piotr Bonarek , Natan Rajtar , Kamil Kamiński

Polyplexes are colloidal systems used in medicine so far almost exclusively in gene therapy as non-viral vectors. Polycation-polyanion reactions responsible for their formation can also bind other biologically valuable polyanions (such as heparin). To realize this potential, it is necessary to describe the relationship of the chemical structure of polycations and the size of the resulting polyplex particles and the subsequent biological manifestations. In work presented here, we postulate that in the case of cationic beta-glucans, the presence of a hydroxypropyl group that can be easily added into the macromolecule structure affects the diameter of the resulting polyplex particles. This phenomenon was measured using dynamic light scattering for systems containing heparin and aptamers and cationic derivatives of grain-derived beta-glucans and modified cellulose. The size of polyplex particles and the type of polycation were also correlated to biological parameters: pro proliferative activity and toxicity towards mammalian cells and antifungal activity against selected pathogenic fungal species (e.g. Candida parapsilosis, Cryptococcus neoformans, Trichophyton tonsurans, Fusarium graeminarum, Lomentospora prolificans and Fusarium solani). The polyplex interaction with cells as a function of time has been characterized using fluorescence microscopy. The study included the calorimetry of the polycation-polyanion reactions described here, as well as the overall physicochemical characteristics of the newly synthesized compounds.

多聚体是医学上使用的胶体系统，迄今为止几乎完全用于基因治疗作为非病毒载体。它们形成的聚阳离子-聚阴离子反应也可以结合其他有生物学价值的聚阴离子（如肝素）。为了实现这一潜力，有必要描述聚阳离子的化学结构和由此产生的多聚颗粒的大小以及随后的生物学表现之间的关系。在这里提出的工作中，我们假设在阳离子β -葡聚糖的情况下，羟基丙基的存在可以很容易地添加到大分子结构中，影响所得到的复合颗粒的直径。这种现象是用动态光散射测量系统含有肝素和适体和阳离子衍生物的谷物衍生β -葡聚糖和改性纤维素。复合颗粒的大小和多阳离子的类型也与生物学参数相关：促增殖活性和对哺乳动物细胞的毒性，以及对特定致病性真菌物种（如副假丝酵母、新型隐球菌、托氏毛癣菌、谷物镰刀菌、增殖性lotospora prolificans和番茄镰刀菌）的抗真菌活性。利用荧光显微镜表征了与细胞的多重相互作用作为时间的函数。本研究包括本文所述的聚阳离子-聚阴离子反应的量热测定，以及新合成化合物的整体物理化学特性。

{"title":"Influence of the polycation structure on the physicochemical and biological properties of polyplex of heparin and aptamers systems","authors":"Małgorzata Tymecka , Magdalena Skóra , Piotr Bonarek , Natan Rajtar , Kamil Kamiński","doi":"10.1016/j.colsurfb.2025.115269","DOIUrl":"10.1016/j.colsurfb.2025.115269","url":null,"abstract":"<div><div>Polyplexes are colloidal systems used in medicine so far almost exclusively in gene therapy as non-viral vectors. Polycation-polyanion reactions responsible for their formation can also bind other biologically valuable polyanions (such as heparin). To realize this potential, it is necessary to describe the relationship of the chemical structure of polycations and the size of the resulting polyplex particles and the subsequent biological manifestations. In work presented here, we postulate that in the case of cationic beta-glucans, the presence of a hydroxypropyl group that can be easily added into the macromolecule structure affects the diameter of the resulting polyplex particles. This phenomenon was measured using dynamic light scattering for systems containing heparin and aptamers and cationic derivatives of grain-derived beta-glucans and modified cellulose. The size of polyplex particles and the type of polycation were also correlated to biological parameters: pro proliferative activity and toxicity towards mammalian cells and antifungal activity against selected pathogenic fungal species (e.g. <em>Candida parapsilosis</em>, <em>Cryptococcus neoformans</em>, <em>Trichophyton tonsurans</em>, <em>Fusarium graeminarum, Lomentospora prolificans</em> and <em>Fusarium solani</em>). The polyplex interaction with cells as a function of time has been characterized using fluorescence microscopy. The study included the calorimetry of the polycation-polyanion reactions described here, as well as the overall physicochemical characteristics of the newly synthesized compounds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"258 ","pages":"Article 115269"},"PeriodicalIF":5.6,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart phase-change CuS@Vk3@LA nanoplatform enables tumor-selective, synergistic chemodynamic and photothermal therapy 智能相变CuS@Vk3@LA纳米平台实现肿瘤选择性，协同化学动力学和光热治疗

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-10 DOI: 10.1016/j.colsurfb.2025.115266

Zhenlong Yuan , Enyu Tang , Hang Wu , Lu Deng , Yuting Zhao , Baofang Zhang , Shengnan Wang , Jing Yu , Lingying Wu

Ovarian cancer therapy remains limited by high recurrence and unsatisfactory responses to current treatments. Here, we developed a multifunctional nanoplatform (CuS@Vk3@LA) that integrates photothermal therapy (PTT), chemodynamic therapy (CDT), and near-infrared (NIR)–controlled drug release. The hollow CuS core serves as both a NIR absorber and a Fenton-like catalyst, enabling simultaneous heat generation and hydroxyl radical production. Uniquely, vitamin K3 (Vk3) was incorporated to enhance endogenous H₂O₂ levels through NQO1-mediated redox cycling, thereby overcoming the substrate shortage that restricts conventional CDT. To prevent premature leakage, lauric acid (LA) was introduced as a thermosensitive phase-change shell, which melts under mild hyperthermia to trigger on-demand release. This rational design establishes a dual oxidative-stress mechanism—ROS amplification and GSH depletion—resulting in synergistic tumor cell killing. Systematic evaluations demonstrated excellent colloidal stability, biocompatibility, and tumor-selective accumulation. In vivo, CuS@Vk3@LA achieved pronounced tumor growth inhibition with minimal systemic toxicity. Collectively, this study provides a precise and safe nanotherapeutic strategy that combines catalytic amplification with photothermal activation, showing strong translational potential for ovarian cancer treatment.

卵巢癌的治疗仍然受到高复发率和目前治疗效果不理想的限制。在这里，我们开发了一个多功能纳米平台（CuS@Vk3@LA），它集成了光热疗法（PTT）、化学动力学疗法（CDT）和近红外（NIR）控制的药物释放。中空的CuS核心既可以作为近红外吸收剂，也可以作为fenton类催化剂，可以同时产生热量和羟基自由基。独特的是，维生素K3 （Vk3）通过nqo1介导的氧化还原循环提高内源性H₂O₂水平，从而克服了限制传统CDT的底物短缺。为了防止过早泄漏，月桂酸（LA）作为热敏相变壳被引入，在轻度高温下熔化以触发按需释放。这种合理的设计建立了双重氧化应激机制- ros扩增和GSH消耗-导致协同杀伤肿瘤细胞。系统评价表明其具有良好的胶体稳定性、生物相容性和肿瘤选择性积累。在体内，CuS@Vk3@LA实现了明显的肿瘤生长抑制和最小的全身毒性。总的来说，这项研究提供了一种精确和安全的纳米治疗策略，将催化扩增与光热活化相结合，在卵巢癌治疗中显示出强大的转化潜力。

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引用次数: 0

Structural and antibacterial mechanisms of the Lysozyme-chlorogenic acid complex against food pathogens 溶菌酶-绿原酸复合物对食品病原菌的结构及抑菌机制研究

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-08 DOI: 10.1016/j.colsurfb.2025.115253

Yutong Zhang , Yu-Xin Chen , Junying Liu , Mao-Cheng Sun , Changhui Zhao

Lysozyme (LYS) and chlorogenic acid (CGA) are natural antibacterial compounds. In this study, we investigated the LYS-CGA colloid complex as a potential strategy to enhance antibacterial activity against Staphylococcus aureus and Salmonella typhimurium. A suite of spectroscopic techniques, including ultraviolet-visible absorption, fluorescence, and circular dichroism spectroscopy was employed to elucidate structural alterations in LYS upon CGA binding. The antibacterial effect was evaluated using a combination of methodologies, including the microbial assay, the scanning electron microscopy and flow cytometry. The binding of CGA induced a transition from α-helical to β-sheet structures in LYS, mediated by hydrogen bonding, hydrophobic, and Van der Waals interactions. This structure demonstrated synergistic antibacterial effect against S. aureus and S. typhimurium, resulting in a substantial reduction in minimum inhibitory concentrations. The complex disrupted bacterial cell membranes, causing leakage of nucleic acids and proteins. Finally, molecular docking was used to further decipher the interaction mechanism of the LYS-CGA complex. The present findings elucidate the structural basis and enhanced antibacterial activity of the LYS-CGA complex.

溶菌酶（LYS）和绿原酸（CGA）是天然抗菌化合物。在这项研究中，我们研究了LYS-CGA胶体复合物作为一种潜在的策略来增强对金黄色葡萄球菌和鼠伤寒沙门菌的抗菌活性。采用紫外-可见吸收光谱、荧光光谱和圆二色光谱等一系列光谱技术来阐明CGA结合后LYS的结构变化。抗菌效果评价采用多种方法，包括微生物测定，扫描电子显微镜和流式细胞术。在氢键、疏水和范德华相互作用的介导下，CGA的结合诱导LYS从α-螺旋结构转变为β-片状结构。该结构显示出对金黄色葡萄球菌和鼠伤寒沙门氏菌的协同抗菌作用，导致最低抑制浓度大幅降低。这种复合物破坏了细菌的细胞膜，导致核酸和蛋白质的泄漏。最后，通过分子对接进一步解读LYS-CGA复合物的相互作用机制。本研究结果阐明了LYS-CGA复合物的结构基础和增强的抗菌活性。

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引用次数: 0

Characterization of cholesterol-containing DOPG bilayers in the presence of the saponins aescin and glycyrrhizin: A NMR and scattering study 七叶皂苷和甘草酸存在下含胆固醇DOPG双分子层的表征：核磁共振和散射研究。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-07 DOI: 10.1016/j.colsurfb.2025.115226

Pia Hägerbäumer , Carina Dargel , Friederike Gräbitz-Bräuer , Ingo Hoffmann , Andreas Mix , Beate Förster , Viktor Sabadasch , Ralf Schweins , Thomas Hellweg

Biological lipid membranes can be mimicked by small unilamellar vesicles (SUVs), which e.g. consist of the negatively charged phospholipid 1,2-dioleoyl-sn-glycero-3-phosphatidylglycerol (DOPG). Due to the unsaturated hydrocarbon chains and hence the low main phase transition temperature, a DOPG membrane is always fluid-like in aqueous solution. By addition of cholesterol to the DOPG model membrane, the membrane stiffness is found to increase. These cholesterol-containing DOPG SUVs are characterized in the presence of varying amounts of the saponins aescin and glycyrrhizin at a temperature of 30 °C by diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY NMR), small-angle neutron scattering (SANS), small- and wide-angle X-ray scattering (SAXS, WAXS), neutron spin echo spectroscopy (NSE), and cryogenic transmission electron microscopy (cryo-TEM). All methods reveal that cholesterol is incorporated into the long-term stable SUVs. Upon saponin addition up to a molar ratio of 1:1 no significant modifications of the SUV size parameters are detected. However, NSE reveals a slight alteration in the membrane elasticity. In sum, the DOSY NMR and scattering results clearly show the coexistence of DOPG-cholesterol SUVs and saponin unimers or micelles and an interaction from the outside of the DOPG vesicles seems to promote the change in the membrane rigidity.

生物脂质膜可以由小的单层囊泡（suv）模拟，例如由带负电荷的磷脂1,2-二油基-sn-甘油-3-磷脂酰甘油（DOPG）组成。由于不饱和烃链和较低的主相变温度，DOPG膜在水溶液中始终呈流体状。通过在DOPG模型膜中加入胆固醇，发现膜刚度增加。采用扩散序核磁共振波谱（DOSY NMR）、小角中子散射（SANS）、小角和广角x射线散射（SAXS， WAXS）、中子自旋回波波谱（NSE）和低温透射电子显微镜（cro - tem）对这些含胆固醇的DOPG suv进行了表征。所有的方法都表明胆固醇与长期稳定的suv有关。当皂素添加到1:1的摩尔比时，SUV尺寸参数没有明显的变化。然而，NSE显示膜弹性略有变化。综上所述，DOSY NMR和散射结果清楚地表明DOPG-胆固醇suv与皂苷单体或胶束共存，并且来自DOPG囊泡外部的相互作用似乎促进了膜刚性的变化。

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引用次数: 0

Light-triggered nanozyme system amplifies oxidative stress for precision cancer therapy 光触发纳米酶系统放大氧化应激精确癌症治疗

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-07 DOI: 10.1016/j.colsurfb.2025.115265

Yalong Wu, Xinquan Gu, Yang Li, Fuquan Jiang, Jiansong Han

Nanozymes exhibiting peroxidase (POD)-like activity and high photothermal conversion efficiency have garnered significant attention for their capacity to generate cytotoxic hydroxyl radicals and provide synergistic therapeutic effects. However, the limited availability of hydrogen peroxide (H₂O₂) in tumor tissues severely restricts the anti-tumor efficacy of nanozymes. To address this challenge, we have designed a light-triggered oxidative stress amplifier system by co-encapsulating Pd nanozyme and camptothecin (CPT) within a polyvinyl alcohol (PVA) matrix. This system enhances anti-tumor activity through self-generated H₂O₂, which amplifies oxidative stress and leverages the excellent photothermal properties of the nanozyme. In the Pd@CPT system, PVA encapsulates the Pd nanozyme and CPT, effectively shielding their toxicity and minimizing off-target effects. Upon exposure to near-infrared (NIR) light, the PVA film is disrupted, leading to the controlled release of the Pd nanozyme and CPT. CPT increases the H₂O₂ level in tumors by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, thereby enhancing the catalytic performance of the Pd nanozyme with POD-like activity. Furthermore, the combination of photothermal therapy and nanozyme-based catalytic therapy synergistically promotes tumor immunogenic cell death and enhances anti-tumor immunity. Our results demonstrate that the novel Pd@CPT system exhibits significant synergistic anti-tumor potential, offering a promising strategy for precision cancer therapy.

具有过氧化物酶（POD）样活性和高光热转化效率的纳米酶因其产生细胞毒性羟基自由基和提供协同治疗作用的能力而受到广泛关注。然而，肿瘤组织中过氧化氢（H2O2）的有限可用性严重限制了纳米酶的抗肿瘤作用。为了解决这一挑战，我们设计了一种光触发氧化应激放大系统，该系统将Pd纳米酶和喜树碱（CPT）共封装在聚乙烯醇（PVA）基质中。该系统通过自身产生H2O2来增强抗肿瘤活性，H2O2可以放大氧化应激，并利用纳米酶优异的光热特性。在Pd@CPT系统中，PVA封装了Pd纳米酶和CPT，有效地屏蔽了它们的毒性并最大限度地减少了脱靶效应。在近红外（NIR）光照射下，PVA膜被破坏，导致Pd纳米酶和CPT的可控释放。CPT通过激活烟酰胺腺嘌呤二核苷酸磷酸（nicotinamide adenine dinucleotide phosphate， NADPH）氧化酶，提高肿瘤中H2O2水平，从而增强具有pod样活性的Pd纳米酶的催化性能。此外，光热疗法和纳米酶催化疗法的结合可协同促进肿瘤免疫原性细胞死亡，增强抗肿瘤免疫。我们的研究结果表明，新的Pd@CPT系统具有显著的协同抗肿瘤潜力，为精确的癌症治疗提供了一个有前途的策略。

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引用次数: 0

Accelerated adhesion of immobilized Saccharomyces cerevisiae on electrophilic interface modified by co-deposition of dopamine and chitosan 多巴胺和壳聚糖共沉积修饰固定化酿酒酵母在亲电界面上的加速粘附

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-07 DOI: 10.1016/j.colsurfb.2025.115252

Jinming Zhang , Jihang Zhang , Lewei Hou , Xiao Zhang , Zhenyu Wang , Jingjing Chen , Jinglan Wu , Pengpeng Yang , Fengxia Zou , Yong Chen , Hanjie Ying , Tianran Li , Wei Zhuang

The development of immobilized biofilm systems through interface engineering represents an effective strategy for bioprocess intensification. Developing functional biofilm carrier interfaces that combine facile fabrication and cost-effectiveness remains a critical challenge in translating immobilized biofilm catalytic systems to industrial applications. In this study, we propose modulating the surface charge microenvironment of the carrier through the encoding of dopamine and chitosan systems to meet the requirements for biofilm system formation. During batch fermentation (50 mL fermentation broth system) using Saccharomyces cerevisiae under a 60 g/L glucose substrate condition, the electrophilic interface carrier exhibited stable biofilm formation, with a maximum density of 66.9 mg/g carrier (33.1 % higher than unmodified carriers) and an immobilization efficiency of 73.1 %. When the glucose concentration was increased to 200 g/L and Saccharomyces cerevisiae was replaced with industrial yeast used in industrial production (200 mL fermentation broth system), the electrophilic interface carrier immobilized fermentation system achieved an ethanol titer of 109.1 g/L, representing 116.2 % of the conventional free-cell fermentation (93.9 g/L), while increasing ethanol productivity to 55.6 %. The immobilized fermentation system exhibited a substrate consumption rate of 6.5 g/L/h, representing a 16.4 % enhancement over conventional free-cell fermentation. The simplicity of carrier fabrication, coupled with its demonstrated performance, establishes a practical foundation for the development of continuous immobilized biofilm systems, offering significant potential for process intensification and industrial-scale implementation.

通过界面工程技术开发固定化生物膜系统是生物过程强化的有效策略。在将固定化生物膜催化系统转化为工业应用的过程中，开发功能生物膜载体界面，结合易于制造和成本效益仍然是一个关键的挑战。在本研究中，我们提出通过编码多巴胺和壳聚糖系统来调节载体的表面电荷微环境，以满足生物膜系统形成的要求。在60 g/L葡萄糖底物条件下，以酵母为原料（50 mL发酵液体系）批量发酵，亲电界面载体的生物膜形成稳定，载体最大密度为66.9 mg/g（比未修饰载体高33.1% %），固定化效率为73.1 %。当葡萄糖浓度增加到200 g/L，工业生产用工业酵母代替酿酒酵母（200 mL发酵液体系）时，亲电界面载体固定化发酵体系的乙醇滴度为109.1 g/L，为常规自由细胞发酵（93.9 g/L）的116.2 %，乙醇产率提高到55.6% %。固定化发酵体系的底物消耗速率为6.5 g/L/h，比传统的自由细胞发酵提高16.4 %。载体制造的简单性，加上其已证明的性能，为连续固定化生物膜系统的发展奠定了实践基础，为工艺强化和工业规模实施提供了巨大的潜力。

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