Colloids and Surfaces B: Biointerfaces最新文献_第10页

The MCM/Lys-Cys nanodevices for the efficient gene delivery: An approach towards MCP1 gene manipulation using CRISPR technology 用于高效基因传递的MCM/Lys-Cys纳米器件：使用CRISPR技术操作smcp1基因的方法。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.colsurfb.2025.115377

Azadeh Rahimi , Ilnaz Rahimmanesh , Navid Abedpoor , Maryam Boshtam , Elham Bidram , Shaghayegh Haghjooy Javanmard , Hossein Khanahmad , Laleh Rafiee , Ashkan Bigham , Mohammad Rafienia , Saeed Karbasi , Laleh Shariati

Breast cancer continues to be the most common malignancy among women worldwide, requiring novel therapeutic approaches. This research investigates an innovative gene delivery strategy employing mesoporous silica nanoparticles (MCM-41) modified with lysine and cysteine (Lys-Cys) for the effective delivery of CRISPR-Cas9 plasmids aimed at the monocyte chemoattractant protein-1 (MCP-1/CCL2) gene. Bioinformatics analysis of the TCGA-BRCA dataset revealed substantial deregulation of CCL2 in breast cancer, underscoring its involvement in tumor growth and inflammation. The MCM/Lys-Cys nanocarrier demonstrated remarkable biocompatibility and effectively encapsulated a plasmid containing GFP, promoting superior cellular uptake in MDA-MB-231 breast cancer cells compared to conventional techniques. Functional experiments demonstrated that CRISPR/Cas9-mediated suppression of CCL2 markedly decreased cell proliferation, migration, and invasion, highlighting the promise of this targeted gene therapy strategy in breast cancer management. The findings indicate that the MCM/Lys-Cys nanosystem presents a viable non-viral approach for precise gene editing, potentially boosting therapeutic efforts against breast cancer by modulating inflammatory pathways.

乳腺癌仍然是全世界妇女中最常见的恶性肿瘤，需要新的治疗方法。本研究研究了一种创新的基因递送策略，利用赖氨酸和半胱氨酸修饰的介孔二氧化硅纳米颗粒（MCM-41）有效递送靶向单核细胞趋化蛋白-1 （MCP-1/CCL2）基因的CRISPR-Cas9质粒。TCGA-BRCA数据集的生物信息学分析揭示了CCL2在乳腺癌中的显著失调，强调其参与肿瘤生长和炎症。MCM/Lys-Cys纳米载体表现出卓越的生物相容性，并有效地封装了含有GFP的质粒，与传统技术相比，促进了MDA-MB-231乳腺癌细胞的细胞摄取。功能实验表明，CRISPR/ cas9介导的CCL2抑制可显著降低细胞增殖、迁移和侵袭，突出了这种靶向基因治疗策略在乳腺癌治疗中的前景。这些发现表明，MCM/Lys-Cys纳米系统为精确的基因编辑提供了一种可行的非病毒方法，可能通过调节炎症途径促进对乳腺癌的治疗。

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引用次数: 0

Baicalein-manganese nanoparticles for antioxidant and osteogenic therapy of bone injury 黄芩素-锰纳米颗粒抗氧化和成骨治疗骨损伤。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-26 DOI: 10.1016/j.colsurfb.2025.115400

Ruqi Wang , Qishu Jin , Zhaoxu Meng , Xiaodong Cao , Yihao Wu , Chen Yang , Jiandong Yuan

Oxidative stress is increasingly recognized as a key pathological driver of skeletal degeneration in diabetes, aging, trauma, and other orthopedic disorders, where excessive reactive oxygen species (ROS) compromise osteoblast and bone marrow mesenchymal stem cell (BMSC) function while promoting osteoclast activity. Natural polyphenols such as baicalein (BA) exhibit potent antioxidant effects but are hindered by poor solubility and limited stability. Here, we report a series of BA-X self-assembled nanoparticles, in which X represents osteogenic metal ions including Mg^2 + , Mn²⁺, Cu²⁺, Zn²⁺, and Sr²⁺. Among these, BA-Mn demonstrated the most robust radical-scavenging activity in DPPH, ABTS, and multiple ROS-targeted assays. BA-Mn exhibited nanoscale dimensions, uniform coordination, and excellent cytocompatibility, significantly reducing intracellular ROS and protecting H₂O₂-injured BMSCs. Functionally, BA-Mn restored osteogenic differentiation under oxidative stress, enhancing alkaline phosphatase activity, mineral deposition, and osteogenic gene expression. Transcriptomic analysis further revealed concurrent activation of cell cycle, PI3K-Akt, and MAPK signaling pathways, alongside suppression of ferroptosis and ROS-related carcinogenesis. Collectively, these findings establish BA-Mn as a multifunctional nanoplatform that integrates antioxidant defense with osteogenic stimulation, offering a promising therapeutic approach for oxidative stress-related bone disorders.

氧化应激越来越被认为是糖尿病、衰老、创伤和其他骨科疾病中骨骼退化的关键病理驱动因素，其中过多的活性氧（ROS）在促进破骨细胞活性的同时损害成骨细胞和骨髓间充质干细胞（BMSC）的功能。黄芩素（BA）等天然多酚具有强大的抗氧化作用，但由于溶解度差和稳定性有限而受到阻碍。在这里，我们报道了一系列BA-X自组装纳米颗粒，其中X代表成骨金属离子，包括Mg2 +，Mn2+, Cu2+， Zn2+和Sr2+。其中，BA-Mn在DPPH、ABTS和多种ros靶向检测中显示出最强大的自由基清除活性。BA-Mn具有纳米级尺寸、均匀的配位性和良好的细胞相容性，可显著降低细胞内ROS，保护h2o2损伤的骨髓间充质干细胞。功能上，BA-Mn恢复氧化应激下的成骨分化，增强碱性磷酸酶活性、矿物质沉积和成骨基因表达。转录组学分析进一步揭示了细胞周期、PI3K-Akt和MAPK信号通路的同步激活，以及铁凋亡和ros相关癌变的抑制。总的来说，这些发现表明BA-Mn是一种多功能纳米平台，将抗氧化防御与成骨刺激结合起来，为氧化应激相关的骨骼疾病提供了一种有希望的治疗方法。

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引用次数: 0

Surface modification of PET Fiber: Evaluation of the synergistic effect of a thermostable engineered cutinase with DBD plasma pretreatment PET纤维的表面改性：热稳定性工程角质酶与DBD血浆预处理协同作用的评价。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-30 DOI: 10.1016/j.colsurfb.2025.115408

Afsaneh Zarei , Farzaneh Alihosseini , Farzaneh Jafary

Poly (ethylene terephthalate) (PET) is a widely used synthetic polymer in textiles, but its hydrophobicity limits its applications despite excellent mechanical properties. Enzymatic hydrolysis provides a sustainable and environmentally friendly approach for PET surface modification, but its efficiency is restricted by the polymer’s high crystallinity and limited enzyme accessibility. In this study, a synergistic plasma-enzyme treatment strategy was developed to enhance PET fiber surface hydrophilicity. Molecular dynamics (MD) simulations were employed to identify thermally unstable regions in leaf-branch compost cutinase (LCC), guiding the design of a thermostable variant, KI (T60K/N122I). The engineered KI exhibited a 10 °C improvement in thermal stability and a 1.33-fold increase in hydrolytic efficiency on amorphous PET compared to the native enzyme. PET fibers were subjected to ten sequential plasma–enzyme treatment cycles (90 min, 70 °C each), varying plasma exposure time (90 s and 180 s) and reactive gas type (O₃ or O₂). Surface analyses revealed that pretreatment with O₃ plasma significantly enhanced enzyme activity by promoting higher surface roughness and introducing more oxygen-containing functional groups. The combination of O₃ plasma and KI resulted in 1.5-fold higher water absorption and 1.2-fold higher color strength relative to O₂ plasma–treated samples. Gravimetric measurements confirmed greater mass loss for the O₃–KI system, attributable to enhanced thermal robustness and substrate affinity of the engineered enzyme. Overall, the cyclic plasma–enzyme process demonstrated superior performance compared to conventional modification methods, providing an efficient and sustainable route for functionalizing PET surfaces.

聚对苯二甲酸乙酯（PET）是一种广泛应用于纺织品的合成聚合物，尽管其具有优异的机械性能，但其疏水性限制了其应用。酶水解为PET表面改性提供了一种可持续和环保的方法，但其效率受到聚合物的高结晶度和酶可及性的限制。在这项研究中，开发了一种协同血浆酶处理策略来增强PET纤维的表面亲水性。采用分子动力学（MD）模拟方法确定了叶枝堆肥皮化酶（LCC）的热不稳定区，指导了KI （T60K/N122I）热稳定型的设计。与天然酶相比，工程KI的热稳定性提高了10 °C，对非晶PET的水解效率提高了1.33倍。PET纤维经过10个连续的血浆酶处理周期（每次90 min， 70 °C），不同的血浆暴露时间（90 s和180 s）和反应气体类型（O₃或O₂）。表面分析表明，O₃等离子体预处理通过提高表面粗糙度和引入更多含氧官能团显著增强了酶的活性。与O₂等离子体处理的样品相比，O₃等离子体和KI相结合的样品的吸水性提高了1.5倍，颜色强度提高了1.2倍。重量测量证实，O₃-KI体系的质量损失更大，这是由于工程酶的热稳健性和底物亲和力增强。总的来说，与传统的修饰方法相比，循环等离子体酶工艺表现出优越的性能，为PET表面功能化提供了一种有效和可持续的途径。

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引用次数: 0

Oral environment targeting sutures for refractory oral wound 口腔环境靶向缝合治疗难治性口腔创面

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-25 DOI: 10.1016/j.colsurfb.2025.115388

Shuze Wang , Jin Wang , Yue Wang , Xintong Ye , Liyun Zhang , Guangqi Yan , Xue Wang

The use of sutures for stable wound closure in the humid, microbiologically complex oral environment is critical for mucosal healing. However, traditional absorbable sutures often loosen due to oral movements, leading to micro-gaps and accelerated microbial colonization, which disrupts healing. Inspired by spider silk's "expansion-induced supercontraction" in humidity, this study develops tea polyphenols self-contracting sutures to enhance mechanical reinforcement and repair efficiency. This next-generation degradable suture features a spider silk-inspired elastic network: polyvinyl alcohol (PVA) chains provide entropy-driven elastic recovery, while an ion-crosslinked network of sodium alginate and Ca²⁺ serves as a moisture-responsive unit. When dry, the network maintains pre-tension. Upon hydration, water molecules competitively coordinate with alginate and Ca²⁺, reducing crosslink density. This allows the PVA network to actively contract within four hours post-surgery, approximating wound edges, minimizing gaps, and reducing bacterial invasion. This next-generation degradable suture exhibits robust antibacterial activity, with 90.3 % inhibition against E. coli and 95.6 % against S. aureus. It also exerts significant biological regulation: compared to controls, it upregulates anti-inflammatory IL-10 expression by 2.41-fold, enhances oxidative stress scavenging capacity by 1.87-fold, and promotes angiogenesis. In vivo tests on difficult oral wounds show this next-generation degradable suture accelerates healing, reduces scarring, and improves tissue regeneration quality, especially in infected wounds. It holds broad potential for minimally invasive oral and maxillofacial surgeries and infection-prone wound repair.

在潮湿、微生物复杂的口腔环境中，使用缝合线稳定地缝合伤口对粘膜愈合至关重要。然而，传统的可吸收缝合线经常因口腔运动而松动，导致微间隙和加速微生物定植，从而破坏愈合。受蜘蛛丝在湿度条件下“膨胀致超收缩”的启发，本研究开发了茶多酚自收缩缝合线，以提高机械加固和修复效率。这种下一代可降解缝合线具有蜘蛛丝启发的弹性网络：聚乙烯醇（PVA）链提供熵驱动的弹性恢复，而海藻酸钠和Ca 2 +的离子交联网络作为水分响应单元。干燥时，网保持预张力。水合作用后，水分子与海藻酸盐和Ca 2 +竞争性地配位，降低了交联密度。这使得PVA网络在术后4小时内主动收缩，接近伤口边缘，最大限度地减少间隙，减少细菌入侵。这种下一代可降解缝线具有强大的抗菌活性，对大肠杆菌的抑制率为90.3 %，对金黄色葡萄球菌的抑制率为95.6% %。具有显著的生物学调节作用：与对照组相比，可上调抗炎IL-10表达2.41倍，提高氧化应激清除能力1.87倍，促进血管生成。对困难口腔伤口的体内试验表明，这种新一代可降解缝线加速愈合，减少疤痕，提高组织再生质量，特别是在感染伤口中。它在微创口腔颌面外科和易感染伤口修复方面具有广阔的潜力。

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引用次数: 0

Recent advances in hydrogel therapy for traumatic brain injury 水凝胶治疗外伤性脑损伤的最新进展。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2026-01-06 DOI: 10.1016/j.colsurfb.2026.115424

Zheng Zou , Mengjia Chen , Jun Liu , Huan Ma , Guobiao Liang , Jingyuan Li

Traumatic brain injury (TBI) remains one of the most devastating neurological disorders, characterized by complex primary mechanical damage and secondary cascades involving inflammation, oxidative stress, and glial scar formation. Conventional therapies offer limited efficacy due to the blood–brain barrier, the inability to reconstruct tissue defects, and poor spatiotemporal drug control. Hydrogels have emerged as a versatile platform for brain repair owing to their high-water content, biocompatibility, tunable mechanics, and injectability. By tailoring their composition and crosslinking mechanisms, hydrogels can mimic the native brain extracellular matrix, fill irregular cavities, and provide mechanical support that matches neural tissue softness. More importantly, functional hydrogels serve as local delivery vehicles for neuroprotective drugs and growth factors, scaffolds for stem cell transplantation, and active regulators of the post-injury microenvironment. Recent advances include bioactive, conductive, and stimuli-responsive hydrogels capable of modulating immune polarization, enhancing angiogenesis, and promoting neurogenesis. Despite encouraging preclinical results, challenges remain in achieving long-term biocompatibility, precise degradation control, and scalable clinical translation. This review summarizes the current progress, underlying mechanisms, and emerging design strategies of hydrogel-based therapies for TBI, highlighting their potential as next-generation biomaterials for neuroregeneration and functional recovery.

创伤性脑损伤（TBI）是最具破坏性的神经系统疾病之一，其特点是复杂的原发性机械损伤和继发性级联反应，包括炎症、氧化应激和神经胶质瘢痕形成。由于血脑屏障，无法重建组织缺陷，以及药物时空控制差，传统疗法的疗效有限。水凝胶由于其高含水量、生物相容性、可调力学和可注射性而成为脑修复的通用平台。通过调整它们的组成和交联机制，水凝胶可以模拟天然的大脑细胞外基质，填充不规则的空洞，并提供与神经组织柔软度相匹配的机械支持。更重要的是，功能水凝胶可以作为神经保护药物和生长因子的局部递送载体，干细胞移植的支架，以及损伤后微环境的主动调节剂。最近的进展包括生物活性、导电性和刺激反应性水凝胶，能够调节免疫极化、增强血管生成和促进神经发生。尽管临床前结果令人鼓舞，但在实现长期生物相容性、精确降解控制和可扩展的临床转化方面仍然存在挑战。本文综述了目前基于水凝胶治疗TBI的进展、潜在机制和新兴设计策略，强调了它们作为下一代神经再生和功能恢复生物材料的潜力。

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引用次数: 0

Integrated analysis of single-cell and bulk RNA-seq data identifies NR4A1-associated macrophages in immunoglobulin A nephropathy 单细胞和大量RNA-seq数据的综合分析确定了免疫球蛋白A肾病中nr4a1相关的巨噬细胞。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115395

Xueqin Zhang , Peng Chao , Lei Zhang, Yang Zhao, Hui Li, Yipeng Zhou, Xue Song, Shufen Yang, Gulimire Muhetaer, Hong Jiang, Chen Lu

Immunoglobulin A nephropathy (IgAN), the most prevalent primary glomerulonephritis worldwide, is characterized by chronic renal inflammation and progressive decline toward end-stage renal disease. In this study, we integrated single-cell and bulk RNA sequencing approaches to systematically elucidate the role of macrophages in IgAN pathogenesis. Single-cell transcriptomic analysis identified 171 macrophage-associated differentially expressed genes and revealed two distinct molecular subtypes with divergent immune and metabolic signatures. Using machine learning algorithms, we constructed a diagnostic model based on eight macrophage-related markers, including NR4A1, which demonstrated robust predictive accuracy (Area Under the Curve > 0.9) across multiple independent validation cohorts. Functional experiments further established NR4A1 as a protective factor in IgAN. Overexpression of NR4A1 in an IgAN mouse model markedly reduced renal fibrosis, macrophage infiltration, and glomerular injury, whereas NR4A1 knockout exacerbated these pathological features. Mechanistically, the transcription factor ZBTB7A, which is upregulated in IgAN, directly suppresses NR4A1 expression through protein interaction. Rescue experiments demonstrated that NR4A1 overexpression mitigates ZBTB7A-induced renal damage. Collectively, these findings identify the ZBTB7A–NR4A1 regulatory axis as a critical mechanism driving IgAN progression and highlight NR4A1 as a potential diagnostic biomarker and therapeutic target for disease prevention.

免疫球蛋白A肾病（IgAN）是世界范围内最常见的原发性肾小球肾炎，其特征是慢性肾脏炎症并逐渐向终末期肾脏疾病发展。在这项研究中，我们整合了单细胞和大量RNA测序方法来系统地阐明巨噬细胞在IgAN发病机制中的作用。单细胞转录组学分析鉴定了171个巨噬细胞相关的差异表达基因，揭示了两种不同的分子亚型，具有不同的免疫和代谢特征。利用机器学习算法，我们构建了一个基于8种巨噬细胞相关标志物的诊断模型，其中包括NR4A1，该模型在多个独立验证队列中显示出强大的预测准确性（曲线下面积> 0.9）。功能实验进一步证实了NR4A1在IgAN中的保护作用。在IgAN小鼠模型中，NR4A1过表达可显著减少肾纤维化、巨噬细胞浸润和肾小球损伤，而NR4A1敲除则加重了这些病理特征。在机制上，IgAN中上调的转录因子ZBTB7A通过蛋白相互作用直接抑制NR4A1的表达。抢救实验证实NR4A1过表达可减轻zbtb7a诱导的肾损害。总的来说，这些发现确定了ZBTB7A-NR4A1调控轴是驱动IgAN进展的关键机制，并突出了NR4A1作为疾病预防的潜在诊断生物标志物和治疗靶点。

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引用次数: 0

Screening and application of a coagulation peptide-KFVLK with rapid hemostatic property 具有快速止血作用的凝血肽kfvlk的筛选与应用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-19 DOI: 10.1016/j.colsurfb.2025.115387

Xin Liu , Zekai Ren , Xin Ding , Han Wu , Yumei Wang , Yang Cao , Hailin Cong , Bing Yu

Wound hemostasis and healing have been important issues faced in wound therapy. Bioactive peptides are widely used in wound therapy due to their good biocompatibility and tunable functionality. Here we prepared Erythrocyte Membrane Chromatography Packing Material (EMCPM) by combining SiO₂ stationary-phase microspheres and cell membranes of erythrocytes through diazotized resin, and screened the coagulation peptide KFVLK from mobile phase peptide libraries, which lyophilized in the form of white solid particles with good biosafety, and could rapidly adsorb erythrocytes and platelets to stop wound bleeding. In the liver hemorrhage model, it could stop wound bleeding within 20 s, which reduced blood loss by 50 % compared with the control group. Meanwhile, it has good performance in promoting wound healing, with a healing rate of 88 % on fibroblasts within 24 h. The wound healing model also verified its performance. In conclusion, this screening method is of great significance for the preparation of coagulation peptides and hemostatic treatment of wounds.

创面止血与愈合一直是创面治疗面临的重要问题。生物活性肽因其具有良好的生物相容性和可调节的功能而广泛应用于伤口治疗。本研究通过重氮化树脂将SiO2固定相微球与红细胞细胞膜结合，制备了红细胞膜色谱包装材料（EMCPM），并从流动相肽库中筛选出凝血肽KFVLK，冻干后形成白色固体颗粒，生物安全性好，能快速吸附红细胞和血小板，起到止血作用。在肝出血模型中，可在20 s内止血，与对照组相比，出血量减少50% %。同时，它具有良好的促进伤口愈合的性能，在24 h内对成纤维细胞的愈合率可达88 %。伤口愈合模型也验证了其性能。综上所述，该筛选方法对凝血肽的制备及伤口止血治疗具有重要意义。

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引用次数: 0

Chemical bonding-driven charge transfer in Z-scheme bimetallic sulfides for rapid wound healing z型双金属硫化物中化学键驱动的电荷转移用于伤口快速愈合。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115396

Jianfang Li , Guoying Jiang , Huijun Meng , Weiduo Zhao , Yuan Tian , Jianlin Xu , Liheng Feng

The rational design of bio-interfaces that efficiently generate reactive oxygen species (ROS) is crucial for antimicrobial therapies. Here, we present a Z-scheme heterojunction of Bi₂S₃-Sv/WS₂, engineered at the interface through in-situ growth to form covalent Bi-S-W bonds. This specific bonding, coupled with introduced sulfur vacancies, creates an asymmetric electron distribution and a built-in electric field. The synergistic effect establishes a direct and rapid pathway for charge carrier transport, which is conclusively verified by femtosecond transient absorption spectroscopy (fs-TAS) to suppress charge recombination and extend carrier lifetimes. Consequently, the system exhibits a significant enhancement in the generation of reactive oxygen species (ROS) under 660 nm light irradiation. The optimized heterojunction demonstrates broad-spectrum antibacterial efficacy, achieving a 99.63 % inhibition rate against both Staphylococcus aureus and Escherichia coli within 15-minute treatment. Furthermore, the material exhibits excellent biocompatibility. This study discloses the role of interfacial chemical bonding and defect engineering in tailoring charge dynamics at the bio-interface, thereby providing a promising strategy for the rational design of highly efficient photocatalytic agents for biomedical applications, such as wound healing and infection control.

合理设计有效产生活性氧（ROS）的生物界面对抗菌治疗至关重要。在这里，我们提出了Bi2S3-Sv/WS2的Z-scheme异质结，通过原位生长在界面上形成共价Bi-S-W键。这种特殊的键，加上引入的硫空位，产生了不对称的电子分布和一个内置的电场。这种协同效应建立了一条直接快速的载流子输运途径，飞秒瞬态吸收光谱（fs-TAS）证实了这种协同效应抑制了电荷复合，延长了载流子寿命。因此，在660 nm光照射下，该体系的活性氧（ROS）的生成显著增强。优化后的异质结具有广谱抗菌效果，在15分钟内对金黄色葡萄球菌和大肠杆菌的抑制率达到99.63 %。此外，该材料还具有良好的生物相容性。本研究揭示了界面化学键和缺陷工程在调整生物界面电荷动力学中的作用，从而为合理设计用于伤口愈合和感染控制等生物医学应用的高效光催化剂提供了一种有前途的策略。

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引用次数: 0

Evaluation of functionalized gold nanoparticles stability in a microfluidic device to mimic a biological environment 功能化金纳米颗粒在模拟生物环境的微流控装置中的稳定性评价。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.colsurfb.2025.115404

Sabina Arias , Natalia Arancibia , Ramón Rial , Juan Ruso , Marcelo J. Kogan , Natalia Hassan

The functionalization of gold nanoparticles (NPs) with polyethylene glycol (PEG) and folic acid improves their stability, biocompatibility, and targeting capacity, essential for biomedical applications. However, conventional characterization methods often overlook the influence of dynamic biological environments. In this study, we investigated the stability of spherical and rod-shaped gold nanoparticles (GNSs and GNRs) under both static and dynamic conditions, utilizing a microfluidic device designed to emulate a biological environment. Nanoparticles were characterized in terms of hydrodynamic size, zeta potential, and surface plasmon resonance in three distinct media: Milli-Q water, phosphate-buffered saline (PBS), and RPMI 1640 cell culture medium supplemented with 10 % fetal bovine serum. Our findings reveal that protein-rich media enhance nanoparticle stability through the formation of a protein corona, whereas PBS promotes aggregation, particularly under static conditions. Notably, GNRs exhibited higher protein adsorption and superior colloidal stability under flow conditions compared to GNSs, a behavior attributed to their anisotropic geometry and increased surface interaction dynamics. This study underscores the relevance of testing nanoparticle behavior under physiologically relevant flow conditions, providing valuable information to optimize nanoparticle design for nanomedicine.

聚乙二醇（PEG）和叶酸功能化金纳米颗粒（NPs）提高了它们的稳定性、生物相容性和靶向能力，这对生物医学应用至关重要。然而，传统的表征方法往往忽略了动态生物环境的影响。在这项研究中，我们利用一个模拟生物环境的微流控装置，研究了球形和棒状金纳米颗粒（GNSs和GNRs）在静态和动态条件下的稳定性。纳米颗粒在三种不同的介质中进行了水动力学大小、ζ电位和表面等离子体共振的表征：milliq水、磷酸盐缓冲盐水（PBS）和添加10% %胎牛血清的RPMI 1640细胞培养基。我们的研究结果表明，富含蛋白质的介质通过形成蛋白质冠来增强纳米颗粒的稳定性，而PBS则促进聚集，特别是在静态条件下。值得注意的是，与GNSs相比，GNRs在流动条件下表现出更高的蛋白质吸附性和优越的胶体稳定性，这一行为归因于它们的各向异性几何结构和更高的表面相互作用动力学。本研究强调了在生理相关流动条件下测试纳米颗粒行为的相关性，为优化纳米药物的纳米颗粒设计提供了有价值的信息。

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引用次数: 0

Combination of transfection incompetent lipids having strikingly different aliphatic chain lengths in a liposome demonstrates superior transfection and produces high titre lentivirus 在脂质体中具有显著不同脂肪链长度的转染不合格脂质的组合表明转染优越，并产生高滴度的慢病毒。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.colsurfb.2025.115402

Yasodha Ramasamy , Ramya Arumugam , Sevanthy Suresh , Gokulnath Mahalingam , Rajesh Kumar Subhaschandra bose , Mohanraj Ramasamy , Ilavarasan Raju , Srujan Marepally

The favourable outcome of gene therapy depends on the developing efficient and safe gene delivery systems. Recently, nonviral vectors have gained considerable attention due to their improved safety profiles and their potential application in viral vector manufacturing. In this study, we report the design, synthesis, and evaluation of novel short-chain (C6) ionizable lipids with varying hydrophobic amino acid headgroups phenylalanine (C6F-IL), tryptophan (C6W-IL), and glycine (C6G-IL) along with a cationic lipid containing twin C12 alkyl chains (C12-CL). Liposomes composed solely of either C6 ionizable amino lipids (C6-IL) or C12-CL were unable to efficiently deliver plasmid DNA. However, an optimized combination of C12-CL and C6 ionizable lipids, particularly C6W-IL (Lipo-3), demonstrated superior transfection efficiency across HEK-293T, SKHEP, and HEPA cell lines, comparable to that of Lipofectamine 3000. Furthermore, when Lipo-3 was applied in lentivirus production, it yielded viral titres equivalent to those obtained using commercial Lipofectamine 3000. These findings demonstrate that a combination of two individually transfection-incompetent lipids can be engineered into a highly efficient liposomal system for gene delivery. This approach provides new opportunities for harnessing existing transfection-incompetent lipids in gene therapy applications.

基因治疗的良好效果取决于开发高效、安全的基因传递系统。近年来，非病毒载体因其安全性的提高和在病毒载体制造中的潜在应用而受到广泛关注。在这项研究中，我们报道了新型短链（C6）可电离脂质的设计、合成和评价，这些脂质具有不同的疏水氨基酸头基苯丙氨酸（C6F-IL）、色氨酸（C6W-IL）和甘氨酸（C6G-IL），以及含有双C12烷基链（C12- cl）的阳离子脂质。仅由C6离子化氨基脂（C6- il）或C12-CL组成的脂质体不能有效地传递质粒DNA。然而，经过优化的C12-CL和C6可电离脂质组合，特别是C6W-IL (lipo3)，在HEK-293T、SKHEP和HEPA细胞系中显示出与Lipofectamine 3000相当的卓越转染效率。此外，当将lipo3应用于慢病毒生产时，其产生的病毒滴度与使用商用Lipofectamine 3000获得的病毒滴度相当。这些发现表明，两种单独转染无能的脂质的组合可以被设计成一个高效的脂质体系统，用于基因传递。这种方法为利用现有的转染无能脂质进行基因治疗提供了新的机会。

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