Colloids and Surfaces B: Biointerfaces最新文献_第6页

A self-adapting DNA-crosslinked hydrogel reprograms macrophage polarization and angiogenesis for synergistic repair of diabetic wounds 自适应dna交联水凝胶重编程巨噬细胞极化和血管生成，用于糖尿病伤口的协同修复

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-20 DOI: 10.1016/j.colsurfb.2026.115465

Wenxin Feng , Yi Gao , Zexin Peng , Hongxu Liu , Qiuwen Yang , Xin Gao , Yi Jin , Xinjian Yang , Enjun Wang

Chronic diabetic wounds continue to be a major therapeutic challenge due to sustained inflammation, impaired angiogenesis, and disrupted extracellular matrix remodeling. Partial conventional dressings and growth factor therapies often act passively or release agents independently, lacking coordinated regulation of immune and vascular responses necessary for effective healing. In this study, we report a self-adapting DNA-crosslinked silk fibroin hydrogel designed to reprogram macrophage polarization and angiogenesis for coordinated diabetic wound repair. The hydrogel integrates L-arginine-loaded bone marrow mesenchymal stem cell-derived exosomes within a pH-responsive DNA conformational switch, in which cholesterol-modified DNA strands undergo an acidic pH-induced duplex–to–i-motif transition. This structural change disrupts hydrogen bonding between complementary strands, enabling precise release of therapeutic cargos in the acidic diabetic wound microenvironment. In vitro, the hydrogel effectively induced macrophage M1-to-M2 repolarization, enhanced endothelial cell proliferation, migration, and angiogenesis. In diabetic mouse models, it accelerated reepithelialization, enhanced CD31 and vascular endothelial growth factor expression, suppressed pro-inflammatory cytokines, and restored collagen deposition. This self-adapting, dual-modulatory hydrogel establishes a self-reinforcing “immune-vascular” feedback loop, enabling synchronized immunoregulation and angiogenesis. The findings highlight a programmable therapeutic strategy for reversing chronic inflammatory microenvironments and promoting functional tissue regeneration in diabetic wounds.

由于持续的炎症、血管生成受损和细胞外基质重塑被破坏，慢性糖尿病伤口仍然是一个主要的治疗挑战。部分传统敷料和生长因子疗法往往被动或独立释放药物，缺乏有效愈合所必需的免疫和血管反应的协调调节。在这项研究中，我们报道了一种自适应dna交联丝素水凝胶，旨在重编程巨噬细胞极化和血管生成，以协调糖尿病伤口修复。该水凝胶将l-精氨酸负载的骨髓间充质干细胞衍生的外泌体整合到ph响应性DNA构象开关中，在该开关中，胆固醇修饰的DNA链经历酸性ph诱导的双链到i基序的转变。这种结构变化破坏了互补链之间的氢键，从而在酸性糖尿病伤口微环境中精确释放治疗物质。在体外，水凝胶可有效诱导巨噬细胞m1 - m2再极化，增强内皮细胞的增殖、迁移和血管生成。在糖尿病小鼠模型中，它能加速再上皮化，增强CD31和血管内皮生长因子的表达，抑制促炎细胞因子，恢复胶原沉积。这种自适应、双调节的水凝胶建立了一个自我强化的“免疫-血管”反馈回路，实现了同步的免疫调节和血管生成。研究结果强调了一种可编程的治疗策略，可以逆转慢性炎症微环境，促进糖尿病伤口的功能性组织再生。

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引用次数: 0

A dual-modal GSH depletion and NIR-triggered nanoplatform for cascade-amplified phototherapy 级联放大光疗的双模态GSH耗尽和nir触发纳米平台。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-19 DOI: 10.1016/j.colsurfb.2026.115461

Shuang Liu , Xingyu Chen , Xiaojin Liu , Hao Zhang , Keyue Zhou , Yuting Shan , Minghui Chen , Dan Wang , Guohui Cheng

In tumor phototherapy, the intracellular antioxidant defenses, such as high concentrations of glutathione (GSH) and inefficient generation of reactive oxygen species (ROS) significantly restrict the therapeutic efficacy. In this research, we construct a dual-modal GSH depletion and near-infrared (NIR)-triggered nanoplatform (Ce6@CDP) for cascade-amplified photothermal therapy (PTT) and photodynamic therapy (PDT). This nanoplatform featured a hollow mesoporous copper sulfide (CuS) core loaded with Ce6 and encapsulated by a disulfide bond-containing polymer (DS-ANPA-PEG). Upon tumor accumulation, the polymer DS-ANPA-PEG reacts with intracellular GSH to form highly reactive quinone methides (QMs), enabling rapid GSH depletion through alkylation. This dual GSH depletion ‌intensifies oxidative stress, potentiating Ce6-based PDT efficacy. Additionally, the CuS carrier exhibits high photothermal conversion performance (η = 39.68 %), enabling‌ PTT under 808 nm laser irradiation ‌for‌ synergistic tumor eradication. Both in vitro and in vivo studies ‌demonstrate that the PDT/PTT combination therapy ‌significantly‌ inhibits tumor growth while maintaining‌ negligible systemic toxicity. Our findings provide a rational design strategy for developing‌ tumor-microenvironment-responsive multimodal nanoplatforms.

在肿瘤光疗中，细胞内的抗氧化防御，如高浓度的谷胱甘肽（GSH）和低效的活性氧（ROS）的产生，严重限制了治疗效果。在这项研究中，我们构建了一个用于级联放大光热治疗（PTT）和光动力治疗（PDT）的双模态GSH耗尽和光动力触发纳米平台（Ce6@CDP）。该纳米平台的特点是中空的介孔硫化铜（cu）核心负载Ce6，并被含二硫键的聚合物（DS-ANPA-PEG）包裹。在肿瘤积聚时，聚合物DS-ANPA-PEG与细胞内谷胱甘肽反应形成高活性的醌类化合物（QMs），通过烷基化使谷胱甘肽快速消耗。这种双重GSH耗竭会加剧氧化应激，增强ce6基PDT的功效。此外，CuS载流子具有较高的光热转换性能（η = 39.68 %），可在808 nm激光照射下实现PTT协同肿瘤根除。体外和体内研究均表明，PDT/PTT联合疗法可显著抑制肿瘤生长，同时维持可忽略的全身毒性。我们的发现为开发肿瘤微环境响应的多模态纳米平台提供了合理的设计策略。

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引用次数: 0

A Mn-incorporated porphyrinic metal-organic framework with near-infrared light responsive photothermal effect and intrinsic ROS scavenging ability for promoted healing of bacteria-infected wounds 一种具有近红外光响应光热效应和内在活性氧清除能力的mn -卟啉金属有机骨架促进细菌感染伤口愈合。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-19 DOI: 10.1016/j.colsurfb.2026.115460

Haihan Song, Mengli Zhang, Zhefeng Qian, Zhengwei Mao, Weijun Tong

Photothermal nanomaterials constitute a promising platform for a broad spectrum of antimicrobial applications. However, the efficacy of photothermal therapy alone is often compromised by associated thermal stress, which elevates mitochondrial reactive oxygen species (ROS) production. The bacterial lysis can also release endotoxins to exacerbate oxidative stress and impede wound healing. Herein, we develop a Mn-incorporated porphyrinic metal–organic framework (Mn@PCN-224). Beyond the previously reported ROS scavenging abilities, it demonstrates outstanding near-infrared responsive photothermal conversion with a photothermal conversion efficiency of 34.1 %. Experimental results and theoretical calculations indicate the incorporation of Mn enhances light absorption, suppresses electron-hole recombination, forms sub-gap transitions and narrows the band gap. Moreover, Mn@PCN-224 exhibits intrinsic superoxide dismutase-like and catalase-like activities unaffected by light exposure, enabling effective scavenging of excess ROS to alleviate inflammation. In the Staphylococcus aureus-infected mouse wound model, Mn@PCN-224 not only eradicates bacteria but also accelerates healing by reducing inflammation, promoting collagen deposition, and stimulating angiogenesis. This synergistic antibacterial and anti-inflammatory therapeutic strategy offers a promising therapeutic paradigm for bacteria-infected wounds.

光热纳米材料为广泛的抗菌应用提供了一个有前途的平台。然而，单独光热疗法的效果经常受到相关热应激的影响，热应激会提高线粒体活性氧（ROS）的产生。细菌裂解还能释放内毒素，加剧氧化应激，阻碍伤口愈合。在此，我们开发了一种mn掺杂卟啉金属有机骨架（Mn@PCN-224）。除了先前报道的活性氧清除能力之外，它还表现出出色的近红外响应光热转换，光热转换效率为34.1% %。实验结果和理论计算表明，Mn的加入增强了光吸收，抑制了电子-空穴复合，形成了子隙跃迁，缩小了带隙。此外，Mn@PCN-224具有不受光照影响的内在超氧化物歧化酶样和过氧化氢酶样活性，能够有效清除过量的ROS以减轻炎症。在金黄色葡萄球菌感染的小鼠伤口模型中，Mn@PCN-224不仅能根除细菌，还能通过减少炎症、促进胶原沉积、刺激血管生成等方式加速愈合。这种协同抗菌和抗炎治疗策略为细菌感染伤口提供了一种有希望的治疗范例。

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引用次数: 0

Biomimetic pore-throat engineered ultrahigh molecular weight polyethylene with sustained tea polyphenol release for infection-resistant joint implant material 仿生孔喉工程超高分子量聚乙烯茶多酚持续释放抗感染关节植入材料。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-17 DOI: 10.1016/j.colsurfb.2026.115458

Kang Li , Yi-Zi Wang , Shao-Peng Zhao , Peiqi Yu , Zhi Qiao , Mengfan Jing , Yaming Wang , Yue Ren , Yu Han , Chuntai Liu

To address the limitations of systemic antibiotics in treating prosthetic joint infections (PJI), ultrahigh molecular weight polyethylene (UHMWPE)-based polyphenol delivery systems have been developed as an antibiotic-free strategy. However, challenges exist in achieving sustained antimicrobial efficacy and enhancing overall performance. To achieve threshold-insensitive sustained release, a biomimetic pore-throat structure inspired by geologic capillary transport mechanisms was engineered within UHMWPE. This structure was constructed through strategic co-incorporation of polyethylene oxide (PEO) and a sub-permeation-threshold tea polyphenol (epigallocatechin gallate, EGCG as representative). PEO-encapsulated EGCG clusters formed expanded pore-throat networks with enhanced distribution uniformity, facilitated by hydrogen bonding between PEO’s ether groups and EGCG’s phenolic hydroxyls. This microstructure enabled swelling-regulated drug release obeying Korsmeyer-Peppas kinetics. The PEO concentration directly modulated sustained EGCG release through capillary action and swelling-erosion. The resulting composites achieved > 80 % antibacterial efficacy against major pathogens while PEO simultaneously formed boundary-hydrated lubrication layers that reduced the friction coefficient to 0.065. Combined with favorable biocompatibility and effective suppression of bacterial-induced inflammation, this biomimetic strategy establishes a material-level, proof-of-concept approach with potential for future development toward infection-resistant orthopedic devices.

为了解决全身抗生素治疗假体关节感染（PJI）的局限性，基于超高分子量聚乙烯（UHMWPE）的多酚递送系统已被开发为一种无抗生素的策略。然而，在实现持续的抗菌功效和提高整体性能方面存在挑战。为了实现阈值不敏感的持续释放，在UHMWPE中设计了一种受地质毛细管输送机制启发的仿生孔喉结构。该结构是通过聚乙烯氧化物（PEO）和亚渗透阈值茶多酚（表没食子儿茶素没食子酸酯，EGCG为代表）的战略性共结合而构建的。PEO封装的EGCG簇形成了扩展的孔喉网络，分布均匀性增强，PEO的醚基团和EGCG的酚羟基之间的氢键促进了这一点。这种微观结构使肿胀调节的药物释放服从Korsmeyer-Peppas动力学。PEO浓度通过毛细管作用和溶胀侵蚀直接调节EGCG的持续释放。复合材料对主要病原菌的抗菌效果达到了> 80 %，同时PEO形成了边界水合润滑层，使摩擦系数降低到0.065。结合良好的生物相容性和有效抑制细菌诱导的炎症，这种仿生策略建立了一种材料水平的概念验证方法，具有未来开发抗感染骨科设备的潜力。

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引用次数: 0

Zinc oxide quantum dots enhanced growth performance and zinc metabolism in weaned piglets 氧化锌量子点提高了断奶仔猪的生长性能和锌代谢。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-17 DOI: 10.1016/j.colsurfb.2026.115456

Xin Li , Jintao Wang , Chen Liang , Wei Fan , Wenshuang Chen , Bing Wu , Liu Chen , Daiwen Chen , Aimin Wu , Xianxiang Wang

The widespread use of conventional high-dose zinc oxide (ZnO) for diarrhea prevention and growth promotion in weaned piglets carries risks of toxicity, bacterial resistance, and environmental harm. This study assessed zinc oxide quantum dots (ZnO-QDs) as a safer, effective alternative. In vitro antibacterial tests demonstrated that, at the same dosage, ZnO-QDs exhibited superior antimicrobial activity compared to conventional ZnO. Subsequent animal trials were conducted using 192 weaned piglets (Duroc × Landrace × Yorkshire; age=21d old; BW=7.70 ± 0.20 kg), which were randomly assigned to four dietary treatment groups (CON, 2-ZnO, 1-ZnO-QDS and 0.5- ZnO-QDS) in a completely randomized design. Results showed that, comparable under the present conditions, lower doses of ZnO-QDs (500 mg/kg, 0.5- ZnO-QDS) provide growth and anti-diarrheal benefits equal to higher doses of conventional ZnO (2000 mg/kg, 2-ZnO) (P > 0.05). ZnO-QDs upregulated jejunal ZIP-1, ZIP-5, ZIP-9 and ZIP-13 gene expression (P < 0.05) for zinc transport and increase total antioxidant capacity in serum (P < 0.01). Importantly, it also improved intestinal health, as evidenced by increased villus height in the duodenum (P < 0.05) and modulation of gut microbiota composition. Substituting conventional ZnO with 500 mg/kg ZnO-QDs could effectively promote piglet growth and reduce diarrhea, while decreasing zinc emissions and environmental impact.

在断奶仔猪中广泛使用传统的大剂量氧化锌（ZnO）来预防腹泻和促进生长，存在毒性、细菌耐药性和环境危害的风险。本研究评估氧化锌量子点（ZnO-QDs）作为一种更安全、有效的替代方案。体外抗菌实验表明，在相同剂量下，ZnO- qds比常规ZnO具有更好的抗菌活性。后续动物试验选用断奶仔猪192头（杜×长×大，年龄=21d，体重=7.70 ± 0.20 kg），采用完全随机设计，随机分为4个饲粮处理组（CON、2-ZnO、1-ZnO-QDS和0.5- ZnO-QDS）。结果表明，在当前条件下，低剂量ZnO- qds（500 mg/kg, 0.5- ZnO- qds）与高剂量常规ZnO（2000 mg/kg, 2-ZnO）具有相同的生长和抗腹泻作用（P > 0.05）。ZnO-QDs上调空肠ZIP-1、ZIP-5、ZIP-9和ZIP-13基因表达(P

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引用次数: 0

Gallic- and ω-3-linolenic-acids-mediated MgO nanoplates enable band-edge tuning and ROS-driven anticancer activity 没食子酸和ω-3-亚麻酸介导的MgO纳米板可以实现带边调谐和ros驱动的抗癌活性。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-16 DOI: 10.1016/j.colsurfb.2026.115455

Ankita Thakur , Ahmed Ahmed Ibrahim , Khalid Mujasam Batoo , Jyoti Gaur , Kasim Sakran Abass , Sanjeev Kumar

Green nanotechnology seeks earth-abundant, low-toxicity oxides that can deliver biomedical benefits with minimal environmental impact. We report the first magnesium oxide (MgO) nanoparticles (NPs) synthesised exclusively from Pinus patula needle waste via a one-pot, aqueous route that requires no organic solvents or post-calcination. Targeted GC–MS profiling pinpoints gallic acid, ω-3 linolenic-acid bis-TMS ether and three mono-linoleoylglycerol derivatives as the dominant reductive/capping agents (Σ > 35 % TIC), while FT-IR confirms they are in-situ chelation to Mg²⁺. The renewable extract simultaneously reduces Mg²⁺, caps nascent nuclei and templates mesoporosity, affording a 78 % yield of well-crystalized platelets (XRD domain 55 ± 3 nm; BET surface area 54 m² g⁻¹; pore diameter 3.8 nm). Oxygen-vacancy enrichment contracts the bulk 7.8 eV gap to an indirect 3.24 eV; the resulting band edges (–0.29 V and +1.81 V vs NHE, pH 7) favour superoxide but suppress hydroxyl-radical formation, enabling stimulus-dependent redox switching. In vitro, the particles trigger intrinsic apoptosis in MCF-7 cells with an IC₅₀ of 71 ng mL⁻¹ and a 32-fold Bax/Bcl-2 shift, yet exhibit negligible haemolysis toward erythrocytes. Antioxidant assays reveal a DPPH•-scavenging IC₅₀ of 25 µg mL⁻¹ lower than any green-synthesised MgO reported to date. Comprehensive GC-MS, FT-IR and time-resolved XRD tracking link the vacancy stabilization and ripening inhibition to the coordinated gallic-acid/flavolipid corona.

绿色纳米技术寻求地球上丰富的、低毒性的氧化物，这种氧化物可以在最小的环境影响下提供生物医学效益。我们报道了第一个氧化镁纳米颗粒（MgO）纳米颗粒（NPs）完全由松针废料通过一锅，不需要有机溶剂或后煅烧的水溶液路线合成。定向GC-MS分析确定没食子酸、ω-3亚麻酸双tms醚和三种单亚麻油基甘油衍生物是主要的还原/封盖剂（Σ > 35% TIC），而FT-IR证实它们是原位螯合到Mg 2 +的。可再生提取物同时降低了Mg 2 +，盖住了新生细胞核和模板的介孔，提供了78%的结晶良好的薄片（XRD域55 ±3 nm； BET表面积54 m²g⁻¹；孔径3.8 nm）。氧空位富集将本体7.8 eV的间隙间接压缩到3.24 eV；由此产生的能带边缘（-0.29 V和+1.81 V vs NHE, pH 7）有利于超氧化物，但抑制羟基自由基的形成，从而实现刺激依赖性氧化还原开关。在体外，这些颗粒触发MCF-7细胞的内在凋亡，其IC₅₀为71 ng mL⁻¹ 和32倍的Bax/Bcl-2位移，但对红细胞的溶血作用可以忽略。抗氧化测试显示，清除DPPH的IC₅₀为25 µg mL⁻¹ ，低于迄今为止报道的任何绿色合成的MgO。综合GC-MS， FT-IR和时间分辨XRD跟踪将空位稳定和成熟抑制与没食子酸/黄脂配位冕联系起来。

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引用次数: 0

Membrane-mimicking surfaces modulate the heme pocket structure and oxygen affinity in myoglobin: A surface-enhanced resonance Raman study 膜模拟表面调节血红素口袋结构和氧亲和力在肌红蛋白：表面增强共振拉曼研究。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-16 DOI: 10.1016/j.colsurfb.2026.115457

Ulises A. Zitare , Andresa Messias , Santiago Di Lella , Magalí F. Scocozza , Darío A. Estrin , Luciana Capece , Daniel H. Murgida

In this study, we investigated how membrane-mimicking surfaces modulate the structure and oxygen affinity of myoglobin (Mb) using surface-enhanced resonance Raman spectroscopy under electrochemical control. Nanostructured silver electrodes functionalized with self-assembled monolayers of varying charge and composition were employed to model basic features of the outer mitochondrial membrane in a controlled fashion. Molecular dynamics simulations show that the interactions of Mb with these surfaces are mediated by lysine-rich domains in helix F and the CD region. These interactions induce subtle structural changes in Mb’s heme pocket, of both the ferric and ferrous protein, that imply the loss of the proximal ligand, water and molecular oxygen, respectively. The fraction of ferrous OxyMb that retains the oxygen ligand in the biomimetic complexes exhibits spectral features consistent with a weakened Fe–O₂ bond and decreased O₂ affinity. Overall, our findings show that Mb’s transient interaction with a mitochondrial membrane model facilitates O₂ release through fine structural modulation, providing a possible molecular basis for how such interactions might influence oxygen delivery in cells.

在这项研究中，我们利用电化学控制下的表面增强共振拉曼光谱研究了膜模拟表面如何调节肌红蛋白（Mb）的结构和氧亲和力。纳米结构的银电极功能化的自组装单层不同的电荷和组成，以控制方式模拟线粒体外膜的基本特征。分子动力学模拟表明，Mb与这些表面的相互作用是由螺旋F和CD区富含赖氨酸的结构域介导的。这些相互作用引起Mb血红素口袋的细微结构变化，包括铁蛋白和亚铁蛋白，这意味着近端配体、水和分子氧的损失。在仿生复合物中保留氧配体的氧化亚铁的部分表现出与Fe-O2键减弱和O2亲和力降低一致的光谱特征。总之，我们的研究结果表明，Mb与线粒体膜模型的短暂相互作用通过精细的结构调节促进了O2的释放，为这种相互作用如何影响细胞中的氧气传递提供了可能的分子基础。

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引用次数: 0

pH-assisted ratiometric surface-enhanced Raman scattering for reliable creatinine quantification in serum ph辅助比例表面增强拉曼散射用于可靠的血清肌酐定量。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-16 DOI: 10.1016/j.colsurfb.2026.115454

Dechan Lu , Xiaoqi Zhou , Dandan Su , Jundong Li , Huizhen Lin , Rongmao Qiu

Abnormal serum creatinine levels are a key clinical biomarker for kidney dysfunction, making its accurate monitoring essential for early diagnosis and prevention. Current commercial kits based on the creatine kinase method are often affected by matrix interference, limiting their reliability in clinical settings. To address this issue, we developed a pH- assisted ratiometric surface-enhanced Raman scattering (SERS) approach for precise creatinine detection in biological samples. We synthesized core-shell nanoparticles (Au@MPA@Ag NPs) with 3-mercaptopropionic acid (MPA) as the internal standard. Under optimized alkaline conditions (pH = 9), the adsorption of creatinine onto the substrate was significantly enhanced, leading to a strong and reproducible SERS response. Moreover, the internal standard-calibrated Raman intensity exhibited a strong linear correlation with creatinine concentration, achieving the limit of detection as 3.3 μM. As a proof of concept, this method was successfully applied to quantify creatinine in human serum. The recovery rates ranged from 98 % to 107 %, demonstrating high consistency with results obtained using a commercial creatinine assay kit. This method provides a clinically accurate and reliable for creatinine monitoring, offering strong potential for point-of-care testing.

血清肌酐水平异常是肾功能障碍的重要临床生物标志物，对其进行准确监测对早期诊断和预防至关重要。目前基于肌酸激酶方法的商用试剂盒经常受到基质干扰的影响，限制了其在临床环境中的可靠性。为了解决这个问题，我们开发了一种pH辅助比例表面增强拉曼散射（SERS）方法，用于精确检测生物样品中的肌酐。我们以3-巯基丙酸（MPA）为内标合成了核壳纳米粒子（Au@MPA@Ag NPs）。在优化后的碱性条件下（pH = 9），肌酐在底物上的吸附显著增强，产生了强烈的、可重复的SERS响应。内标拉曼强度与肌酐浓度呈较强的线性相关，检出限为3.3 μM。作为概念验证，该方法已成功应用于人血清中肌酸酐的定量。回收率范围为98 %至107 %，与商用肌酐测定试剂盒的结果高度一致。该方法提供了临床准确可靠的肌酐监测，为即时检测提供了强大的潜力。

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引用次数: 0

Design, characterization, and in vitro evaluation of Eudragit-coated aminated mesoporous silica nanoparticles loaded with pterostilbene for colon delivery 设计、表征和体外评价载紫菀芪的涂膜胺化介孔二氧化硅纳米颗粒结肠递送

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-15 DOI: 10.1016/j.colsurfb.2026.115452

Nikhil Raut , Manasa N. , Swornahuti Panda, Victor Hmingthangsanga, Subramanian Natesan

Ulcerative colitis (UC), a chronic inflammatory colon disease, often requires long-term care. Pterostilbene (PTB), a naturally occurring substance with strong anti-inflammatory and antioxidant properties, has shown therapeutic potential; however, its poor aqueous solubility and instability at stomach pH limit its clinical application. A colon-targeted drug delivery system loaded with PTB and coated with Eudragit S100 (EU) was developed using mesoporous silica nanoparticles (MSN). The MSN were surface-aminated using 3-aminopropyl triethoxysilane (APTES) to obtain aminated mesoporous silica nanoparticles (AMSN), which provided pH-responsive release and enhanced drug binding. FT-IR spectroscopy confirmed the encapsulation of the drug and amine functionalization through characteristic spectral shifts. X-ray diffraction (XRD) showed decreased crystallinity of PTB, suggesting effective molecular dispersion within the MSN matrix. Differential scanning calorimetry (DSC) was used to confirm the amorphous form of the encapsulated medication. Morphological study using SEM and TEM revealed consistently spherical, porous nanoparticles. Thermogravimetric analysis (TGA) demonstrated the formulation's thermal stability, and Brunauer-Emmett-Teller (BET) analysis showed high surface area and pore volume, both of which are advantageous for efficient drug loading. In vitro release investigations confirmed a pH-dependent, extended release of the drug under intestinal conditions. Additionally, pro-inflammatory cytokines (TNF-α and IL-6) were significantly reduced in the ELISA assays of LPS-stimulated HT-29 cells, demonstrating the formulation's anti-inflammatory efficacy. The development of colon-specific delivery of pterostilbene, the Eudragit-coated PTB-loaded AMSN (Eu-PTB-AMSN), effectively addresses the primary shortcomings of conventional PTB formulations, including low solubility, stomach instability, and systemic adverse effects, while offering a practical therapeutic approach for the treatment of UC.

溃疡性结肠炎（UC）是一种慢性炎症性结肠疾病，通常需要长期护理。紫檀芪（PTB）是一种天然存在的具有强抗炎和抗氧化特性的物质，已显示出治疗潜力；但其水溶性差，胃pH值不稳定，限制了其临床应用。利用介孔二氧化硅纳米颗粒（MSN）制备了一种载PTB并包被Eudragit S100 （EU）的结肠靶向给药系统。利用3-氨基丙基三乙氧基硅烷（APTES）对微球表面进行胺化，得到胺化介孔二氧化硅纳米颗粒（AMSN），该纳米颗粒具有ph响应释放和增强药物结合的特性。傅里叶变换红外光谱通过特征光谱位移证实了药物的包封和胺的功能化。x射线衍射（XRD）表明PTB的结晶度降低，表明在MSN基体中存在有效的分子分散。用差示扫描量热法（DSC）确定了包封药物的无定形。利用扫描电镜和透射电镜对纳米颗粒进行了形态学研究，结果表明纳米颗粒呈球形、多孔。热重分析（TGA）表明该制剂具有良好的热稳定性；比表面积和孔容分析（BET）表明该制剂具有较高的比表面积和孔容，有利于高效载药。体外释放调查证实了ph依赖性，延长了药物在肠道条件下的释放。此外，在lps刺激的HT-29细胞的ELISA检测中，促炎细胞因子（TNF-α和IL-6）显著降低，证明该配方具有抗炎功效。紫芪二苯乙烯的结肠特异性递送，eudragte涂层PTB负载AMSN （Eu-PTB-AMSN）的开发，有效解决了传统PTB配方的主要缺点，包括低溶解度，胃不稳定和全身不良反应，同时为UC的治疗提供了一种实用的治疗方法。

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引用次数: 0

Visualizing intracellular and extracellular cell-mineral interactions in a three-dimensional breast tumor model 可视化三维乳腺肿瘤模型中细胞内和细胞外细胞-矿物质相互作用。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2026-01-15 DOI: 10.1016/j.colsurfb.2026.115450

Amit Cohen , Einat Nativ-Roth , Netta Vidavsky

Microcalcifications (MCs) in breast tissue, with particularly high prevalence in precancerous lesions, such as ductal carcinoma in situ (DCIS), represent important diagnostic markers for cancer screening. Two primary crystal types, hydroxyapatite (HA) and calcium oxalate dihydrate (COD), occur within breast tumor microenvironments. Evidence from clinical samples has demonstrated that COD is usually found as individual crystals, whereas HA tends to aggregate. Notably, MC crystal types and properties are linked to malignancy, with COD crystals being associated with benign lesions. In-vitro studies have shown that COD crystals can suppress cancerous behavior, whereas HA often promotes it. However, crystal–cell interactions in tumor microenvironments that may explain differential responses to COD and HA remain poorly investigated. Thus, to examine mineral–cell interactions based on cell malignancy potential and crystal type, a three-dimensional multicellular model consisting of precancerous and cancerous human cell lines was employed. Through cryo-scanning electron microscopy (cryo-SEM), we visualized mineral–biological interfaces and showed that cellular types and processes govern crystal aggregation patterns and distribution. Both precancerous and invasive models exhibited enhanced COD crystal packing, while HA crystals formed predominantly loose structures with notable malignancy-dependent variations. In all cases, crystals were found both extra- and intracellularly, the latter being located inside vesicles in some cases, especially for COD crystals. The predominance of intracellular vesicles containing COD crystals rather than HA crystals across both cell types may reflect enhanced cellular processes that promote reduced malignant behavior. These findings may explain why seemingly similar calcium-containing minerals associate differently with malignancy and induce distinct cellular responses.

乳腺组织中的微钙化（MCs）在癌前病变（如导管原位癌（DCIS））中发病率特别高，是癌症筛查的重要诊断指标。两种主要的晶体类型，羟基磷灰石（HA）和草酸钙二水合物（COD），发生在乳房肿瘤微环境中。来自临床样本的证据表明，COD通常是单个晶体，而透明质酸则倾向于聚集。值得注意的是，MC晶体类型和性质与恶性肿瘤有关，而COD晶体与良性病变有关。体外研究表明，COD晶体可以抑制癌变行为，而透明质酸通常会促进癌变。然而，肿瘤微环境中晶体-细胞相互作用可能解释COD和HA的不同反应的研究仍然很少。因此，为了研究基于细胞恶性潜能和晶体类型的矿物-细胞相互作用，采用了一个由癌前和癌前人类细胞系组成的三维多细胞模型。通过低温扫描电镜（cryo-SEM），我们可视化了矿物-生物界面，并显示了细胞类型和过程控制晶体聚集模式和分布。癌前病变和侵袭性模型均显示COD晶体堆积增强，而HA晶体主要形成松散结构，具有明显的恶性依赖性。在所有病例中，晶体均在细胞外和细胞内发现，后者在某些情况下位于囊泡内，特别是COD晶体。两种细胞类型中含有COD晶体的细胞内囊泡的优势大于HA晶体，这可能反映了促进恶性行为减少的细胞过程的增强。这些发现可以解释为什么看似相似的含钙矿物质与恶性肿瘤的关联不同，并诱导不同的细胞反应。

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引用次数: 0