Pub Date : 2025-12-09DOI: 10.1016/j.colsurfb.2025.115360
Qiaozheng Wang , Xiaofei Liu , Yongguang Yu , Xianwei Meng , Hongshan Zhong
Microwave ablation (MWA) therapy has gained prominence as an effective yet minimally invasive hyperthermic method for the treatment of hepatocellular carcinoma (HCC). The upregulation of programmed death ligand 1 (PD-L1) and insufficient antitumor immune response induced by thermal injury, however, substantially limit the long-term efficacy of MWA. Suppression of PD-L1 combined with reactive oxygen species (ROS)-enhanced immunogenic cell death (ICD) mediated antitumor immunity is anticipated to improve the MWA prognosis. In this study, a Sunitinib (SUN)-loaded Fe-Cu MOF was designed to prevent tumor recurrence following MWA. The released Sunitinib effectively inhibits PD-L1 expression, which is upregulated by MWA-induced hyperthermic injury. Moreover, the MWA-enhanced dynamic sensitization of Fe-Cu MOF increases ROS production, thereby promoting stronger ICD and enhancing the SUN anti-PD-L1 effect. The presence of Cu and Fe with T1/T2 MR imaging properties enables real-time image-guided monitoring of MWA. Notably, the Fe-Cu MOF@PEG@SUN nanocomposites effectively counteracted MWA-induced PD-L1 upregulation and amplified the extent of ICD post-MWA, thereby enhancing the SUN-mediated anti-PD-L1 immune response and promoting antitumor immunity. Hence, this study offers a promising strategy and theoretical foundation for the integration of diagnostic imaging with MWA-based therapy for HCC.
{"title":"Suppressing hyperthermia-induced up-regulated PD-L1 with a Sunitinib loaded Fe-Cu MOF: Strengthening immunogenic cell death to sensitize anti-PD-L1 effect following microwave ablation of hepatocellular carcinoma","authors":"Qiaozheng Wang , Xiaofei Liu , Yongguang Yu , Xianwei Meng , Hongshan Zhong","doi":"10.1016/j.colsurfb.2025.115360","DOIUrl":"10.1016/j.colsurfb.2025.115360","url":null,"abstract":"<div><div>Microwave ablation (MWA) therapy has gained prominence as an effective yet minimally invasive hyperthermic method for the treatment of hepatocellular carcinoma (HCC). The upregulation of programmed death ligand 1 (PD-L1) and insufficient antitumor immune response induced by thermal injury, however, substantially limit the long-term efficacy of MWA. Suppression of PD-L1 combined with reactive oxygen species (ROS)-enhanced immunogenic cell death (ICD) mediated antitumor immunity is anticipated to improve the MWA prognosis. In this study, a Sunitinib (SUN)-loaded Fe-Cu MOF was designed to prevent tumor recurrence following MWA. The released Sunitinib effectively inhibits PD-L1 expression, which is upregulated by MWA-induced hyperthermic injury. Moreover, the MWA-enhanced dynamic sensitization of Fe-Cu MOF increases ROS production, thereby promoting stronger ICD and enhancing the SUN anti-PD-L1 effect. The presence of Cu and Fe with T<sub>1</sub>/T<sub>2</sub> MR imaging properties enables real-time image-guided monitoring of MWA. Notably, the Fe-Cu MOF@PEG@SUN nanocomposites effectively counteracted MWA-induced PD-L1 upregulation and amplified the extent of ICD post-MWA, thereby enhancing the SUN-mediated anti-PD-L1 immune response and promoting antitumor immunity. Hence, this study offers a promising strategy and theoretical foundation for the integration of diagnostic imaging with MWA-based therapy for HCC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115360"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding biomolecular coronas that spontaneously occur around liposomes in biological fluids is critical as both the lipid and protein coronas influence liposome behavior in biological systems. Herein, PEGylated liposomes were pre-incubated at varying plasma concentrations followed by in vitro dynamic simulation incubation. It was found that increasing plasma concentration from 10 % to 300 % resulted in reduced liposomes uptake by immune cells. Most plasma-derived lipids were found to be retained in the coronas but with altered abundances. Lipids such as CE 18:1 and PC 16:0/18:2 exhibited plasma pre-incubation concentration-dependent changes. Changes in the plasma concentration resulted in the emergence of unique proteins in the final protein corona. Using cross-linking mass spectrometry with two crosslinkers and time-limited proteolysis-mass spectrometry, the associations between plasma pre-incubation concentrations and the topological network of the protein corona, the molecular orientation of proteins including alpha-actinin-4, apolipoprotein A1, etc., on the liposome surface, as well as domain proximity, were identified based on peptide-level structural resolution. Among the detected protein complexes with inter-protein crosslinks, over 85 % were not documented in the BioGRID and STRING databases, and more than 90 % of these complexes failed to align with the predictive models generated by AlphaFold Multimer. These results underscore the characteristic weak interactions between proteins within the protein corona on the surface of PEGylated liposomes. The biomolecular corona on the surface of PEGylated liposomes retains a historical imprint of the microenvironment it has experienced, ultimately shaping the authentic biological identity of the liposomes.
{"title":"Plasma pre-incubation: Concentration-dependent regulation of the biomolecular corona on PEGylated liposome and cellular uptake","authors":"Ziyi Zheng, Zhihua Shen, Chaohua Feng, Guo Xie, Wenli Liu, Ziqiang Pan, Guiliang Tan","doi":"10.1016/j.colsurfb.2025.115355","DOIUrl":"10.1016/j.colsurfb.2025.115355","url":null,"abstract":"<div><div>Understanding biomolecular coronas that spontaneously occur around liposomes in biological fluids is critical as both the lipid and protein coronas influence liposome behavior in biological systems. Herein, PEGylated liposomes were pre-incubated at varying plasma concentrations followed by in <em>vitro</em> dynamic simulation incubation. It was found that increasing plasma concentration from 10 % to 300 % resulted in reduced liposomes uptake by immune cells. Most plasma-derived lipids were found to be retained in the coronas but with altered abundances. Lipids such as CE 18:1 and PC 16:0/18:2 exhibited plasma pre-incubation concentration-dependent changes. Changes in the plasma concentration resulted in the emergence of unique proteins in the final protein corona. Using cross-linking mass spectrometry with two crosslinkers and time-limited proteolysis-mass spectrometry, the associations between plasma pre-incubation concentrations and the topological network of the protein corona, the molecular orientation of proteins including alpha-actinin-4, apolipoprotein A1, etc., on the liposome surface, as well as domain proximity, were identified based on peptide-level structural resolution. Among the detected protein complexes with inter-protein crosslinks, over 85 % were not documented in the BioGRID and STRING databases, and more than 90 % of these complexes failed to align with the predictive models generated by AlphaFold Multimer. These results underscore the characteristic weak interactions between proteins within the protein corona on the surface of PEGylated liposomes. The biomolecular corona on the surface of PEGylated liposomes retains a historical imprint of the microenvironment it has experienced, ultimately shaping the authentic biological identity of the liposomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115355"},"PeriodicalIF":5.6,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-08DOI: 10.1016/j.colsurfb.2025.115353
Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang
The persistent threat of bacterial infections, which contribute to significant global morbidity and mortality, highlights the urgent demand for developing advanced antimicrobial materials. Herein, octopod-shaped silver-gold alloy nanostructures (Ag-Au octopods) were synthesized through a controlled deposition of gold onto silver octopods. Ag-Au octopods can convert near-infrared (NIR) light into thermal energy with photothermal conversion efficiency of 41.5 %. They also exhibited peroxidase-like nanozyme activity, catalysing the decomposition of trace amounts of H2O2 to produce significant levels of reactive oxygen species (ROS). Notably, this catalytic activity was further enhanced upon exposure to NIR light. During in vitro antibacterial tests, a dose of 32 µg mL−1 of Ag–Au octopods, together with H2O2 and 10 min of NIR irradiation, achieved a bactericidal rate greater than 99.0 %. Mechanistic investigations revealed that the antimicrobial effect arises from the disruption of bacterial membrane integrity, leakage of intracellular nucleic acids, a rise in intracellular ROS, and subsequent oxidative stress. The results present an effective and synergistic antibacterial strategy using the combined photothermal and nanozyme activities, offering a promising direction for next-generation antibacterial applications.
细菌感染的持续威胁导致了全球显著的发病率和死亡率，这凸显了开发先进抗菌材料的迫切需求。本文通过控制金沉积在银章鱼体上，合成了章鱼形银金合金纳米结构（Ag-Au octopods）。Ag-Au章鱼可以将近红外（NIR）光转化为热能，光热转换效率为41.5 %。它们还表现出类似过氧化物酶的纳米酶活性，催化微量H2O2的分解，产生大量的活性氧（ROS）。值得注意的是，这种催化活性在近红外光照射下进一步增强。在体外抗菌试验中，32 µg mL - 1 Ag-Au章鱼体，H2O2和10 min的近红外照射，杀菌率大于99.0 %。机制研究表明，抗菌作用源于细菌膜完整性的破坏、细胞内核酸的泄漏、细胞内ROS的增加以及随后的氧化应激。结果表明，利用光热和纳米酶的联合活性，可以有效地协同抗菌，为下一代抗菌应用提供了一个有前途的方向。
{"title":"Octopod-shaped silver–gold alloy for NIR-driven synergistic photothermal and catalytic bacterial ablation","authors":"Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang","doi":"10.1016/j.colsurfb.2025.115353","DOIUrl":"10.1016/j.colsurfb.2025.115353","url":null,"abstract":"<div><div>The persistent threat of bacterial infections, which contribute to significant global morbidity and mortality, highlights the urgent demand for developing advanced antimicrobial materials. Herein, octopod-shaped silver-gold alloy nanostructures (Ag-Au octopods) were synthesized through a controlled deposition of gold onto silver octopods. Ag-Au octopods can convert near-infrared (NIR) light into thermal energy with photothermal conversion efficiency of 41.5 %. They also exhibited peroxidase-like nanozyme activity, catalysing the decomposition of trace amounts of H<sub>2</sub>O<sub>2</sub> to produce significant levels of reactive oxygen species (ROS). Notably, this catalytic activity was further enhanced upon exposure to NIR light. During in vitro antibacterial tests, a dose of 32 µg mL<sup>−1</sup> of Ag–Au octopods, together with H<sub>2</sub>O<sub>2</sub> and 10 min of NIR irradiation, achieved a bactericidal rate greater than 99.0 %. Mechanistic investigations revealed that the antimicrobial effect arises from the disruption of bacterial membrane integrity, leakage of intracellular nucleic acids, a rise in intracellular ROS, and subsequent oxidative stress. The results present an effective and synergistic antibacterial strategy using the combined photothermal and nanozyme activities, offering a promising direction for next-generation antibacterial applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115353"},"PeriodicalIF":5.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-08DOI: 10.1016/j.colsurfb.2025.115347
Jun Xu , Qinqing Xie , Yin Wang , Yaxin Zheng , Xuan Luo , Keming Xu , Wenying Zhong
Hydrogels have attracted extensive attention in the field of drug delivery due to their biocompatibility and tunable properties. However, their limited mechanical strength and uncontrollable drug release behavior remain major obstacles to clinical translation. Inspired by the hierarchical structure of diatoms, we report an in situ mineralized hydrogel (Fmoc-1-OH/AuNPs) based on self-assembling peptides. The hydrogel is formed through the coupling interactions between the peptide side chains and Au³ ⁺ ions. Within the mineralized matrix, gold nanoparticles (AuNPs) not only serve as structural enhancers but also facilitate photothermal effects and on-demand release of doxorubicin hydrochloride (DOX·HCl). Specifically, DOX release is accelerated upon laser irradiation and decelerated when the laser is turned off, demonstrating a reversible and externally controllable release profile. Notably, the Fmoc-1-OH/AuNPs·DOX hydrogel exhibits superior mechanical integrity, photothermal stability, and freeze-drying resistance compared to conventional physically blended hydrogels. Further in vitro and in vivo experiments confirm the enhanced antitumor efficacy of Fmoc-1-OH/AuNPs·DOX, which is mediated by a synergistic combination of chemotherapy (via DOX) and photothermal therapy (via AuNPs). In contrast, monotherapy fails to achieve comparable therapeutic outcomes under the same dosage. This study presents a promising strategy for constructing multifunctional nanocomposite hydrogels with on-demand drug delivery capabilities and improved structural performance.
{"title":"Biomineralized self-assembled peptide metallo-hydrogels: Enhanced stability, mechanical properties, and mild-temperature photothermal effects facilitate controlled drug release and combined tumor therapy","authors":"Jun Xu , Qinqing Xie , Yin Wang , Yaxin Zheng , Xuan Luo , Keming Xu , Wenying Zhong","doi":"10.1016/j.colsurfb.2025.115347","DOIUrl":"10.1016/j.colsurfb.2025.115347","url":null,"abstract":"<div><div>Hydrogels have attracted extensive attention in the field of drug delivery due to their biocompatibility and tunable properties. However, their limited mechanical strength and uncontrollable drug release behavior remain major obstacles to clinical translation. Inspired by the hierarchical structure of diatoms, we report an in situ mineralized hydrogel (Fmoc-1-OH/AuNPs) based on self-assembling peptides. The hydrogel is formed through the coupling interactions between the peptide side chains and Au³ ⁺ ions. Within the mineralized matrix, gold nanoparticles (AuNPs) not only serve as structural enhancers but also facilitate photothermal effects and on-demand release of doxorubicin hydrochloride (DOX·HCl). Specifically, DOX release is accelerated upon laser irradiation and decelerated when the laser is turned off, demonstrating a reversible and externally controllable release profile. Notably, the Fmoc-1-OH/AuNPs·DOX hydrogel exhibits superior mechanical integrity, photothermal stability, and freeze-drying resistance compared to conventional physically blended hydrogels. Further in vitro and in vivo experiments confirm the enhanced antitumor efficacy of Fmoc-1-OH/AuNPs·DOX, which is mediated by a synergistic combination of chemotherapy (via DOX) and photothermal therapy (via AuNPs). In contrast, monotherapy fails to achieve comparable therapeutic outcomes under the same dosage. This study presents a promising strategy for constructing multifunctional nanocomposite hydrogels with on-demand drug delivery capabilities and improved structural performance.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115347"},"PeriodicalIF":5.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-08DOI: 10.1016/j.colsurfb.2025.115357
Zhimin Mo , Jiexi Liang , Qi Xu , Qing Li , Zushun Xu
The acidic tumor microenvironment (TME) frequently diminishes the overall uptake of chemotherapeutic drugs, leading to inefficient drug delivery and tumor resistance. To address this challenge, this study constructed a theranostic nanoplatform (PDA@ACC-DOX-Gd³⁺, abbreviated as PADG) via a simple alkaline vapor diffusion method. This system is designed to alkalize the acidic TME and allows for Magnetic resonance imaging /X-ray computed Tomography (MRI/CT) dual-modal imaging-guided photothermal therapy combined with alkalization-enhanced chemotherapy. The strategy employs in situ alkaline-induced polymerization to form a stable polydopamine (PDA) coating both within and on the surface of amorphous calcium carbonate (ACC), thereby overcoming the inherent instability, rapid dissolution, and recrystallization of ACC in aqueous environments. The resulting PADG not only neutralizes the TME through proton consumption, reducing the reversed pH gradient across tumor cell membranes and improving the utilization of weakly basic drugs, but also enhances tumor chemosensitivity through PDA-mediated photothermal therapy. Both in vitro and in vivo results confirm that PADG exhibits excellent dual-modal MRI/CT imaging performance, effectively consumes protons to alkalize the TME for improved drug efficacy, and achieves significant synergistic photothermal/chemotherapeutic antitumor outcomes.
{"title":"MRI/CT dual-model guided multifunctional acid neutralization nanoplatform for tumor alkalization combined photothermal-chemotherapy","authors":"Zhimin Mo , Jiexi Liang , Qi Xu , Qing Li , Zushun Xu","doi":"10.1016/j.colsurfb.2025.115357","DOIUrl":"10.1016/j.colsurfb.2025.115357","url":null,"abstract":"<div><div>The acidic tumor microenvironment (TME) frequently diminishes the overall uptake of chemotherapeutic drugs, leading to inefficient drug delivery and tumor resistance. To address this challenge, this study constructed a theranostic nanoplatform (PDA@ACC-DOX-Gd³⁺, abbreviated as PADG) via a simple alkaline vapor diffusion method. This system is designed to alkalize the acidic TME and allows for Magnetic resonance imaging /X-ray computed Tomography (MRI/CT) dual-modal imaging-guided photothermal therapy combined with alkalization-enhanced chemotherapy. The strategy employs <em>in situ</em> alkaline-induced polymerization to form a stable polydopamine (PDA) coating both within and on the surface of amorphous calcium carbonate (ACC), thereby overcoming the inherent instability, rapid dissolution, and recrystallization of ACC in aqueous environments. The resulting PADG not only neutralizes the TME through proton consumption, reducing the reversed pH gradient across tumor cell membranes and improving the utilization of weakly basic drugs, but also enhances tumor chemosensitivity through PDA-mediated photothermal therapy. Both <em>in vitro</em> and <em>in vivo</em> results confirm that PADG exhibits excellent dual-modal MRI/CT imaging performance, effectively consumes protons to alkalize the TME for improved drug efficacy, and achieves significant synergistic photothermal/chemotherapeutic antitumor outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115357"},"PeriodicalIF":5.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-08DOI: 10.1016/j.colsurfb.2025.115354
Yu-Yao Liu , Marko Dobricic , Claudio Intini , Fergal J. O'Brien , Javier LLorca , Mónica Echeverry-Rendón
The repair of large traumatic bone defects remains a huge challenge in orthopedic clinics due to the complicated environment of bone healing involving bone regeneration and vascularization in the defect region. This is even more pronounced with an aging population worldwide. To address this, a novel interface-engineered scaffold was developed by integrating a bone-mimetic collagen type I/nano-hydroxyapatite (CI-nHA) matrix with a 3D-printed poly(ε-caprolactone)-polyethylene glycol 20k-poly(ε-caprolactone) (PCL-PEG20k-PCL, PCE20kC) triblock copolymer framework. The scaffold formed biofunctional interfaces with both enhanced mechanical support and promoted cell-material interaction. It exhibited interconnected multi-scale pores and a compressive modulus of ∼37 MPa, comparable to cancellous bone. After culturing with preosteoblasts (MC3T3) under osteogenic conditions for 4 weeks, it showed promoted osteoblast proliferation, differentiation and matrix mineralization. The reinforced architecture further upregulated osteogenic transcription factors of RUNX2 and BMP-2. Moreover, when cultured with endothelial cells, it promoted early angiogenic activity within 5 days, indicating interface-mediated vascularization. Furthermore, when subjected to mechanical stimulation in a bioreactor with simulated physiological mechanical condition, the reinforced scaffold supported osteoblast viability and enhanced early mineralization evidenced by increasing gene expression of ALP and OCN after 1 week of intermittent mechanical stimulation. Overall, this interface-engineered scaffold integrates precise 3D architecture with collagen-functionalized surfaces to effectively support bone regeneration under both static and mechanical conditions, highlighting its translational potential for large bone defect repair.
{"title":"Interface-engineered 3D-printed PCEC/collagen composite scaffold for large bone defect repair under static and mechanical stimulation","authors":"Yu-Yao Liu , Marko Dobricic , Claudio Intini , Fergal J. O'Brien , Javier LLorca , Mónica Echeverry-Rendón","doi":"10.1016/j.colsurfb.2025.115354","DOIUrl":"10.1016/j.colsurfb.2025.115354","url":null,"abstract":"<div><div>The repair of large traumatic bone defects remains a huge challenge in orthopedic clinics due to the complicated environment of bone healing involving bone regeneration and vascularization in the defect region. This is even more pronounced with an aging population worldwide. To address this, a novel interface-engineered scaffold was developed by integrating a bone-mimetic collagen type I/nano-hydroxyapatite (CI-nHA) matrix with a 3D-printed poly(ε-caprolactone)-polyethylene glycol 20k-poly(ε-caprolactone) (PCL-PEG20k-PCL, PCE<sub>20k</sub>C) triblock copolymer framework. The scaffold formed biofunctional interfaces with both enhanced mechanical support and promoted cell-material interaction. It exhibited interconnected multi-scale pores and a compressive modulus of ∼37 MPa, comparable to cancellous bone. After culturing with preosteoblasts (MC3T3) under osteogenic conditions for 4 weeks, it showed promoted osteoblast proliferation, differentiation and matrix mineralization. The reinforced architecture further upregulated osteogenic transcription factors of RUNX2 and BMP-2. Moreover, when cultured with endothelial cells, it promoted early angiogenic activity within 5 days, indicating interface-mediated vascularization. Furthermore, when subjected to mechanical stimulation in a bioreactor with simulated physiological mechanical condition, the reinforced scaffold supported osteoblast viability and enhanced early mineralization evidenced by increasing gene expression of ALP and OCN after 1 week of intermittent mechanical stimulation. Overall, this interface-engineered scaffold integrates precise 3D architecture with collagen-functionalized surfaces to effectively support bone regeneration under both static and mechanical conditions, highlighting its translational potential for large bone defect repair.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115354"},"PeriodicalIF":5.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.colsurfb.2025.115350
Luis F.O. Silva
{"title":"Letter to the Editor of “Proanthocyanidins modification of the mineralized collagen scaffold based on synchronous self-assembly/mineralization for bone regeneration”","authors":"Luis F.O. Silva","doi":"10.1016/j.colsurfb.2025.115350","DOIUrl":"10.1016/j.colsurfb.2025.115350","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115350"},"PeriodicalIF":5.6,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.colsurfb.2025.115348
Muzaffaruddin Ahmed Madny , Khushwant S. Yadav
Oral drug delivery, the most patient friendly administration route offers convenience and compliance but faces formidable biological barriers. Enzymatic degradation, mucosal entrapment, efflux transport and extensive first-pass metabolism drastically reduce the effectiveness of sensitive therapeutics including peptides, proteins, nucleic acids and vaccines. Conventional formulations often fail to overcome these challenges highlighting the need for innovative approaches. Biomimetic drug delivery has emerged as a transformative strategy. By emulating structures and functions from cells, membranes, exosomes, viruses and gut microbiota these systems achieve immune evasion, mucus penetration, site-specific targeting and stimulus-responsive release. Such approaches improve formulation stability and in vivo absorption but also promise precise and patient centric therapies. This review provides a comprehensive overview of biomimetic oral systems highlighting their mechanisms, design principles and translational potential. Recent advances include cell membrane-coated nanoparticles for tumor targeting and immune modulation, exosome-inspired carriers for protein and RNA transport, virus-like particles (VLPs) for oral vaccines, and mucoadhesive or mucus-penetrating polymers modeled on pathogen strategies. Complementary pH, enzyme and redox-responsive platforms exploit gastrointestinal (GI) microenvironments to ensure controlled release. Emerging tools such as bioinspired computational modeling, 3D/4D printing, organoid-on-chip models and CRISPR/Cas-based platforms accelerate optimization and clinical translation. Although most technologies remain in preclinical development, early findings demonstrate superior pharmacokinetics, therapeutic efficacy, and safety over conventional systems. This article critically examines biomimetic oral drug delivery addressing advances and underlying mechanisms including regulatory considerations and future directions. They stand poised to form the foundation of next-generation precision therapeutics.
{"title":"Biomimetic oral drug delivery: Translating nature’s design into therapeutic innovation","authors":"Muzaffaruddin Ahmed Madny , Khushwant S. Yadav","doi":"10.1016/j.colsurfb.2025.115348","DOIUrl":"10.1016/j.colsurfb.2025.115348","url":null,"abstract":"<div><div>Oral drug delivery, the most patient friendly administration route offers convenience and compliance but faces formidable biological barriers. Enzymatic degradation, mucosal entrapment, efflux transport and extensive first-pass metabolism drastically reduce the effectiveness of sensitive therapeutics including peptides, proteins, nucleic acids and vaccines. Conventional formulations often fail to overcome these challenges highlighting the need for innovative approaches. Biomimetic drug delivery has emerged as a transformative strategy. By emulating structures and functions from cells, membranes, exosomes, viruses and gut microbiota these systems achieve immune evasion, mucus penetration, site-specific targeting and stimulus-responsive release. Such approaches improve formulation stability and in vivo absorption but also promise precise and patient centric therapies. This review provides a comprehensive overview of biomimetic oral systems highlighting their mechanisms, design principles and translational potential. Recent advances include cell membrane-coated nanoparticles for tumor targeting and immune modulation, exosome-inspired carriers for protein and RNA transport, virus-like particles (VLPs) for oral vaccines, and mucoadhesive or mucus-penetrating polymers modeled on pathogen strategies. Complementary pH, enzyme and redox-responsive platforms exploit gastrointestinal (GI) microenvironments to ensure controlled release. Emerging tools such as bioinspired computational modeling, 3D/4D printing, organoid-on-chip models and CRISPR/Cas-based platforms accelerate optimization and clinical translation. Although most technologies remain in preclinical development, early findings demonstrate superior pharmacokinetics, therapeutic efficacy, and safety over conventional systems. This article critically examines biomimetic oral drug delivery addressing advances and underlying mechanisms including regulatory considerations and future directions. They stand poised to form the foundation of next-generation precision therapeutics.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115348"},"PeriodicalIF":5.6,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-04DOI: 10.1016/j.colsurfb.2025.115346
Tomomi Mihara, Yuuka Fukui, Keiji Fujimoto
Liposomes are valuable drug and cosmetic carriers but face limitations in stability and active loading from external phases. Polymer-coated liposomes (liponanocapsules) have improved robustness, and this study advances the approach by acetylating chitosan-coated liposomes to form chitin-deposited capsules, leveraging chitin’s biocompatibility and insolubility to enhance the structural and colloidal stability in organic solvents and oils. This represents the first report of suspensions of liposome-based capsules in such media. Anionic liposomes prepared from dilauroyl phosphatidyl acid acquire a hydrodynamic diameter increase from 100 to 190 nm and shift in ζ-potential from negative to positive upon chitosan deposition. Surface acetylation of the deposited chitosan with sodium acetate and a condensing agent yields chitin-deposited liposomes (Lipo[-]-chitin), with the degree of acetylation (DA) controlled by changing the reagent concentration. Lipo[-]-chitin shows a shift in the phase transition of the lipid membrane to a higher temperature owing to the suppression of lipid fluidity, suggesting that the capsules become rigid and robust. The hydrodynamic diameter of Lipo[-]-chitin in water increases with the DA because of capsule aggregation. By contrast, Lipo[-]-chitin becomes smaller in mixtures of water and water-miscible organic solvents, such as dimethyl sulfoxide (DMSO), ethanol, and acetone, indicating a positive impact of acetylation on the colloidal stability. In mixtures of water with DMSO or ethanol, a higher DA reduces the colloidal stability, whereas the opposite trend is observed in acetone. These results indicate that the colloidal stability of the acetylated capsules is strongly governed by the DA. Notably, Lipo[-]-chitin with a higher DA can be suspended in 100 % ethanol without aggregation or rupture. The solvent can then be replaced with poorly water-soluble organic solvents, such as isododecane. This enabled active loading of α-tocopherol as a lipophilic cargo into the capsule. Overall, surface acetylation of chitosan-coated liposomes produces chitin-deposited nanocapsules with enhanced solvent resistance, offering a promising platform for pharmaceutical, cosmetic, and nanocomposite applications.
{"title":"Suspension of liposome-based nanocapsules in organic solvents via surface acetylation of chitosan-deposited liposomes","authors":"Tomomi Mihara, Yuuka Fukui, Keiji Fujimoto","doi":"10.1016/j.colsurfb.2025.115346","DOIUrl":"10.1016/j.colsurfb.2025.115346","url":null,"abstract":"<div><div>Liposomes are valuable drug and cosmetic carriers but face limitations in stability and active loading from external phases. Polymer-coated liposomes (liponanocapsules) have improved robustness, and this study advances the approach by acetylating chitosan-coated liposomes to form chitin-deposited capsules, leveraging chitin’s biocompatibility and insolubility to enhance the structural and colloidal stability in organic solvents and oils. This represents the first report of suspensions of liposome-based capsules in such media. Anionic liposomes prepared from dilauroyl phosphatidyl acid acquire a hydrodynamic diameter increase from 100 to 190 nm and shift in <em>ζ</em>-potential from negative to positive upon chitosan deposition. Surface acetylation of the deposited chitosan with sodium acetate and a condensing agent yields chitin-deposited liposomes (Lipo[-]-chitin), with the degree of acetylation (DA) controlled by changing the reagent concentration. Lipo[-]-chitin shows a shift in the phase transition of the lipid membrane to a higher temperature owing to the suppression of lipid fluidity, suggesting that the capsules become rigid and robust. The hydrodynamic diameter of Lipo[-]-chitin in water increases with the DA because of capsule aggregation. By contrast, Lipo[-]-chitin becomes smaller in mixtures of water and water-miscible organic solvents, such as dimethyl sulfoxide (DMSO), ethanol, and acetone, indicating a positive impact of acetylation on the colloidal stability. In mixtures of water with DMSO or ethanol, a higher DA reduces the colloidal stability, whereas the opposite trend is observed in acetone. These results indicate that the colloidal stability of the acetylated capsules is strongly governed by the DA. Notably, Lipo[-]-chitin with a higher DA can be suspended in 100 % ethanol without aggregation or rupture. The solvent can then be replaced with poorly water-soluble organic solvents, such as isododecane. This enabled active loading of α-tocopherol as a lipophilic cargo into the capsule. Overall, surface acetylation of chitosan-coated liposomes produces chitin-deposited nanocapsules with enhanced solvent resistance, offering a promising platform for pharmaceutical, cosmetic, and nanocomposite applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115346"},"PeriodicalIF":5.6,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-03DOI: 10.1016/j.colsurfb.2025.115344
Boye Zhang , Hongfei Wang , Liping Yu , Yingyu Ma , Guodong Ren , Sufang Ma , Lihong Li , Lixia Guo , Shuming Xu , Lili Yan , Haipeng Diao , Chengwu Zhang , Dongguang Qin , Tianle Yao , Wen Liu
Photodynamic therapy (PDT) continues to face significant challenges, including optimizing photosensitizers, ensuring adequate oxygen supply, and promoting reactive oxygen species (ROS) production, especially within the tumor microenvironment (TME) characterized by low-oxygen and high glucose metabolism. In this study, a cell membrane biomimetic nanoplatform (CM@CHG) had been designed to enable nitric oxide (NO) self-supply and cyclic cascade therapy. The NO-releasing red fluorescence carbon dots (RCDs) were synthesized from neutral red and L-arginine. RCDs was used as photosensitizer and sonosensitizer, combined with glucose oxidase (GOx), and then disguised as esophageal cancer (EC) cell membrane to form CM@CHG, which had good homology targeting properties. In terms of the cascade reaction, GOx decomposed glucose to produce H₂O₂, which not only alleviated tumor hypoxia, but also promoted RCDs to produce NO for gas therapy. MnO₂ was decomposed by high concentration of glutathione (GSH) and treated with Mn²⁺ as a chemodynamic therapy (CDT). At the same time, the fluorescence of RCDs was restored to realize fluorescence imaging. The results of in vitro and in vivo experiments showed that CM@CHG had chemodynamic, photodynamic, sonodynamic, Gas therapy multimodal performance, good biocompatibility, and effectively improved the anti-tumor effect. Transcriptome analysis confirmed that CM@CHG significantly suppressed the expression of both anti-apoptotic and pro-metastatic genes, while significantly up-regulating both pro-apoptotic and anti-metastatic genes. This study provided an innovative and efficient strategy for the diagnosis and treatment of EC, which had potential application in the field of nanomedicine.
{"title":"Cascade responsive cell membrane biomimetic nanoplatform for synergistic therapy of esophageal cancer","authors":"Boye Zhang , Hongfei Wang , Liping Yu , Yingyu Ma , Guodong Ren , Sufang Ma , Lihong Li , Lixia Guo , Shuming Xu , Lili Yan , Haipeng Diao , Chengwu Zhang , Dongguang Qin , Tianle Yao , Wen Liu","doi":"10.1016/j.colsurfb.2025.115344","DOIUrl":"10.1016/j.colsurfb.2025.115344","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) continues to face significant challenges, including optimizing photosensitizers, ensuring adequate oxygen supply, and promoting reactive oxygen species (ROS) production, especially within the tumor microenvironment (TME) characterized by low-oxygen and high glucose metabolism. In this study, a cell membrane biomimetic nanoplatform (CM@CHG) had been designed to enable nitric oxide (NO) self-supply and cyclic cascade therapy. The NO-releasing red fluorescence carbon dots (RCDs) were synthesized from neutral red and <span>L</span>-arginine. RCDs was used as photosensitizer and sonosensitizer, combined with glucose oxidase (GOx), and then disguised as esophageal cancer (EC) cell membrane to form CM@CHG, which had good homology targeting properties. In terms of the cascade reaction, GOx decomposed glucose to produce H₂O₂, which not only alleviated tumor hypoxia, but also promoted RCDs to produce NO for gas therapy. MnO₂ was decomposed by high concentration of glutathione (GSH) and treated with Mn²⁺ as a chemodynamic therapy (CDT). At the same time, the fluorescence of RCDs was restored to realize fluorescence imaging. The results of <em>in vitro</em> and <em>in vivo</em> experiments showed that CM@CHG had chemodynamic, photodynamic, sonodynamic, Gas therapy multimodal performance, good biocompatibility, and effectively improved the anti-tumor effect. Transcriptome analysis confirmed that CM@CHG significantly suppressed the expression of both anti-apoptotic and pro-metastatic genes, while significantly up-regulating both pro-apoptotic and anti-metastatic genes. This study provided an innovative and efficient strategy for the diagnosis and treatment of EC, which had potential application in the field of nanomedicine.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"259 ","pages":"Article 115344"},"PeriodicalIF":5.6,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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