Colloids and Surfaces B: Biointerfaces最新文献_第2页

Dual-targeted and esterase-responsive cyclodextrin-based host-guest nanocomposites for enhanced antitumor therapy 用于增强抗肿瘤治疗的双靶向和酯酶响应环糊精基主客纳米复合材料

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-08 DOI: 10.1016/j.colsurfb.2024.114371

Li Qin , Jianfei Tu , Jiawei Zhao , Yuanke Zhang , Tiancheng Li , Yuqi Zhang , Peng Zhang , Guixia Ling , Jiansong Ji

Conventional chemotherapy drugs are difficult to effectively target tumor tissue, leading to poor treatment outcomes and side effects. Actively targeted and stimuli-responsive nanomedicine greatly improves this situation, allowing for more precise drug accumulation at tumor sites. Herein, carboxymethyl-β-cyclodextrin (CMCD) - based host-guest nanocomposites (NPs) encapsulating hydroxycamptothecin (HCPT) were fabricated, which responded to esterase and had the function of targeting CD 44 receptors and the nucleus. PS-CMCD was firstly synthesized through an amide reaction of protamine (PS) and CMCD to enhance the function of penetrating membrane and nuclear localization. PS-CMCD/HCPT/HA NPs were then prepared by the host-guest complexation of PS-CMCD and HCPT and followed by surface modification of hyaluronic acid (HA) with CD44 receptor-targeting properties. The successful inclusion was also validated through computer simulation. The obtained nanocomposites displayed the esterase-responsive release behaviors of HCPT. Moreover, the synthesized PS-CMCD/HCPT/HA NPs enhanced the intracellular drug uptake due to the tumor cell- and nuclear-mediated targeting. In addition, in vivo application exhibited that PS-CMCD/HCPT/HA NPs realized good antitumor effects. These findings suggested its potential for targeted delivery and more effective tumor therapy.

传统化疗药物难以有效靶向肿瘤组织，导致治疗效果不佳和副作用。而具有主动靶向性和刺激响应性的纳米药物可大大改善这一状况，使药物更精确地在肿瘤部位蓄积。本文制备了基于羧甲基-β-环糊精（CMCD）的主-客纳米复合材料（NPs），其中封装了羟基喜树碱（HCPT），对酯酶有反应，并具有靶向CD 44受体和细胞核的功能。首先通过原胺（PS）和CMCD的酰胺反应合成了PS-CMCD，以增强其穿膜和核定位功能。PS-CMCD/HCPT/HA NPs的制备是通过PS-CMCD和HCPT的主客体复合物，然后在表面修饰具有CD44受体靶向特性的透明质酸（HA）。计算机模拟也验证了这种成功的结合。所获得的纳米复合材料显示了 HCPT 的酯酶响应释放行为。此外，合成的 PS-CMCD/HCPT/HA NPs 通过肿瘤细胞和核介导的靶向作用提高了细胞内的药物吸收。此外，体内应用表明 PS-CMCD/HCPT/HA NPs 具有良好的抗肿瘤效果。这些研究结果表明，PS-CMCD/HCTT/HA NPs具有靶向递送和更有效治疗肿瘤的潜力。

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引用次数: 0

Development of paroxetine loaded nanotransferosomal gel for intranasal delivery with enhanced antidepressant activity in rats 开发用于鼻内给药的帕罗西汀纳米脂质体凝胶，增强大鼠的抗抑郁活性。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114351

Asma Tahir , Sidra Aslam , Saba Sohail , Fakhar ud Din , Ali H. Alamri , Ahmed A. Lahiq , Shaker T. Alsharif , Abdullah Asiri

The aim of this study was to develop paroxetine (PXT) loaded nanotransferosomal gel (PXT-NTFG) for intranasal brain delivery. The process involved fabricating PXT-NTFs (paroxetine-loaded nanotransferosomes) through a thin film hydration method and optimizing them based on parameters such as particle size (PS), zeta potential (ZP), polydispersity index (PDI), and entrapment efficiency (EE). The optimized PXT-NTFs exhibited uniform morphology with a PS of 158.30 ± 2.73 nm, low PDI (0.142 ± 0.072), high ZP (21.00 ± 0.75 mV), and excellent EE (88.09 ± 3.40 %). Characterization through various techniques confirmed the incorporation of PXT into the nanotransferosomes and its conversion to amorphous state. Moreover, PXT-NTFG was formulated with suitable viscosity and mucoadhesive properties. In vitro release studies demonstrated sustained drug release from PXT-NTFG at different pH levels as compared to PXT-NTFs and NTF dispersion. Similarly, ex vivo experiments showed 4 folds enhanced drug permeation from PXT-NTFG when compared with PXT conventional gel. Stability studies indicated that the optimized PXT-NTFs remained stable for four months at 4°C and 25°C. Additionally, improved behavioral outcomes, increased neuronal survival rates, and upregulated brain-derived neurotrophic factor (BDNF) expression was observed in lipopolysaccharide (LPS) induced depressed Sprague-Dawley rats after treatment with PXT-NTFG as compared to PXT-dispersion treated and untreated LPS-control groups. Notably, the formulation led to a significant reduction in brain and plasma TNF-α levels. In conclusion, intranasal PXT-NTFG is a promising formulation with sustained drug release, improved brain targeting and enhanced antidepressant activity.

本研究旨在开发用于鼻内脑部给药的帕罗西汀（PXT）负载纳米转运体凝胶（PXT-NTFG）。研究过程包括通过薄膜水合法制备 PXT-NTFs（帕罗西汀负载纳米转运体），并根据粒度（PS）、ZP、多分散指数（PDI）和夹带效率（EE）等参数对其进行优化。优化后的 PXT-NTFs 形状均匀，PS 为 158.30 ± 2.73 nm，PDI 低（0.142 ± 0.072），ZP 高（21.00 ± 0.75 mV），EE 优良（88.09 ± 3.40 %）。通过各种技术进行的表征证实了 PXT 被纳入纳米转运体并转化为无定形状态。此外，PXT-NTFG 还具有适当的粘度和粘附性。体外释放研究表明，与 PXT-NTFs 和 NTF 分散体相比，PXT-NTFG 在不同 pH 值水平下都能持续释放药物。同样，体内外实验表明，与 PXT 传统凝胶相比，PXT-NTFG 的药物渗透率提高了 4 倍。稳定性研究表明，优化后的 PXT-NTF 在 4°C 和 25°C 温度下可保持稳定四个月。此外，与 PXT 分散液处理组和未经 LPS 处理的对照组相比，使用 PXT-NTFG 处理后，脂多糖（LPS）诱导的 Sprague-Dawley 抑郁大鼠的行为结果得到改善，神经元存活率提高，脑源性神经营养因子（BDNF）表达上调。值得注意的是，该制剂显著降低了脑部和血浆中 TNF-α 的水平。总之，鼻内PXT-NTFG是一种很有前景的制剂，它具有持续的药物释放、更好的脑靶向性和更强的抗抑郁活性。

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引用次数: 0

Design of enzyme immobilized zwitterionic copolymer nanogels and its size effect on electrochemical reaction 酶固定化齐聚物纳米凝胶的设计及其对电化学反应的尺寸影响

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114370

Takehiro Sato, Yixuan Huang, Tsukuru Masuda, Jincai Li, Madoka Takai

For enzyme-based electrochemical devices, an improvement in electron transfer between the enzyme and electrode is important. Thus, we developed a nano-scaled hydrogel that includes an electron mediator and enzyme to realize nano-sized effects that enhance the functions. Three different chain lengths (short, medium, and long) of copolymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) and methacrylic acid N-hydroxysuccinimide ester (MNHS; poly(MPC-co-MNHS), PMS) were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The PMS nanogels can bind to the amino ferrocene (AFc) of the electron mediator and glucose oxidase (GOD) as a catalyst. The mono-dispersive PMS nanogels approximately 200–250 nm in size bound with AFc were prepared with different polymer chain lengths and amounts of AFc (PMMFcX_Y%, X= ‘degree of polymerization, 50, 75, 100’ and Y= ‘AFc feeding ratio against the amount of NHS group in the polymer chain, 50 %, 100 %’). The size of PMMFcX_Y% could be controlled by changing degree of polymerization or AFc feeding ratio. After the modification of GOD to PMMFcX_Y%, their size increased slightly from the original size (ca. 200–250 nm) to approximately 250–300 nm. The catalytic activity of nanogel in dispersed system was higher than that of microgel, indicating that nanogels could improve glucose transport in hydrogel layer. Compared to the catalytic reaction of the PMMFc 75_50 %-GOD nanogel-modified electrodes with that of microgel modified electrode, the current response was improved by decreasing the nanogel size, as evaluated by electrochemical measurements. These results revealed that the smaller nanogels could improve both glucose transport and electron transfer via mediator by smaller size, resulting higher efficiency of enzyme immobilized electrode.

对于以酶为基础的电化学装置而言，改善酶与电极之间的电子传递非常重要。因此，我们开发了一种包含电子介质和酶的纳米级水凝胶，以实现增强功能的纳米级效果。我们通过可逆加成-断裂链转移（RAFT）聚合法合成了由 2-甲基丙烯酰氧乙基磷酰胆碱（MPC）和甲基丙烯酸 N-羟基琥珀酰亚胺酯（MNHS；poly(MPC-co-MNHS)，PMS）组成的三种不同链长（短、中、长）的共聚物。PMS 纳米凝胶可与作为电子介质的氨基二茂铁（AFc）和作为催化剂的葡萄糖氧化酶（GOD）结合。用不同的聚合物链长度和 AFc 含量（PMMFcX_Y%，X="聚合度，50、75、100"，Y="AFc 含量与聚合物链中 NHS 基团含量的比率，50%，100%"）制备了与 AFc 结合的单分散 PMS 纳米凝胶，其尺寸约为 200-250 nm。PMMFcX_Y% 的大小可通过改变聚合度或 AFc 进料比来控制。将 GOD 改性为 PMMFcX_Y% 后，其尺寸从原来的约 200-250 纳米略微增加到约 250-300 纳米。纳米凝胶在分散体系中的催化活性高于微凝胶，表明纳米凝胶可以改善葡萄糖在水凝胶层中的运输。与微凝胶修饰电极相比，PMMFc 75_50 %-GOD 纳米凝胶修饰电极的催化反应随着纳米凝胶尺寸的减小而增强。这些结果表明，较小的纳米凝胶可以通过较小的尺寸改善葡萄糖转运和通过介质的电子传递，从而提高酶固定电极的效率。

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引用次数: 0

Surface-enhanced infrared spectroscopic study of extracellular vesicles using plasmonic gold nanoparticles 利用等离子金纳米粒子对细胞外囊泡进行表面增强红外光谱研究。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114366

Tímea Bebesi , Marcell Pálmai , Imola Csilla Szigyártó , Anikó Gaál , András Wacha , Attila Bóta , Zoltán Varga , Judith Mihály

Extracellular vesicles (EVs), sub-micrometer lipid-bound particles released by most cells, are considered a novel area in both biology and medicine. Among characterization methods, infrared (IR) spectroscopy, especially attenuated total reflection (ATR), is a rapidly emerging label-free tool for molecular characterization of EVs. The relatively low number of vesicles in biological fluids (∼10¹⁰ particle/mL), however, and the complex content of the EVs’ milieu (protein aggregates, lipoproteins, buffer molecules) might result in poor signal-to-noise ratio in the IR analysis of EVs. Exploiting the increment of the electromagnetic field at the surface of plasmonic nanomaterials, surface-enhanced infrared spectroscopy (SEIRS) provides an amplification of characteristic IR signals of EV samples. Negatively charged citrate-capped and positively charged cysteamine-capped gold nanoparticles with around 10 nm diameter were synthesized and tested with blood-derived EVs. Both types of gold nanoparticles contributed to an enhancement of the EVs’ IR spectroscopic signature. Joint evaluation of UV-Vis and IR spectroscopic results, supported by FF-TEM images, revealed that proper interaction of gold nanoparticles with EVs is crucial, and an aggregation or clustering of gold nanoparticles is necessary to obtain the SEIRS effect. Positively charged gold nanoparticles resulted in higher enhancement, probably due to electrostatic interaction with EVs, commonly negatively charged.

细胞外囊泡（EVs）是大多数细胞释放的亚微米级脂质结合颗粒，被认为是生物学和医学的一个新领域。在各种表征方法中，红外（IR）光谱，尤其是衰减全反射（ATR）光谱，是一种迅速崛起的用于表征细胞外囊泡分子特征的无标记工具。然而，由于生物液体中的囊泡数量相对较少（每毫升中只有 1010 个），而且 EVs 的环境（蛋白质聚集体、脂蛋白、缓冲分子）成分复杂，可能导致 EVs 的红外分析信噪比较低。利用等离子纳米材料表面的电磁场增量，表面增强红外光谱（SEIRS）可以放大 EV 样品的特征红外信号。我们合成了直径约为 10 纳米的带负电荷的柠檬酸盐帽金纳米粒子和带正电荷的半胱胺帽金纳米粒子，并用血源性 EV 进行了测试。两种类型的金纳米粒子都有助于增强 EVs 的红外光谱特征。在 FF-TEM 图像的支持下，对紫外可见光谱和红外光谱结果进行的联合评估显示，金纳米粒子与 EVs 的适当相互作用至关重要，金纳米粒子的聚集或团聚是获得 SEIRS 效果的必要条件。带正电荷的金纳米粒子能产生更高的增强效果，这可能是由于它们与通常带负电荷的 EV 发生了静电作用。

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引用次数: 0

Construction of bilayer biomimetic periosteum based on SLA-3D printing for bone regeneration 基于 SLA-3D 打印技术构建用于骨再生的双层仿生骨膜。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114368

Xingguo Zhou , Bin Zou , Qinghua Chen , Gongxian Yang , Qingguo Lai , Xinfeng Wang

An ideal biomimetic periosteum should have excellent biocompatibility to promote osteoclast adhesion and improve osseointegration, which is significant in promoting bone regeneration. In this work, a bionic artificial periosteum printed by the SLA-3D printing was prepared, consisting of a poly (ethylene glycol) diacrylate (PEGDA)/chitosan/tricalcium phosphate (TCP) fibrous layer and a gelatin methacryloyl (GelMA)/ammonium molybdate (Mo) cambium layer. Distinct surface characteristics were achieved on both sides of the biomimetic periosteum. Among them, the fibrous layer has high mechanical properties and low porosity, which is conducive to preventing the pulling of muscle tissues and the invasion of soft tissues. The cambium layer has a porous structure and bioactive factors that can effectively promote osteogenic differentiation of preosteoblasts. Combined with mild photothermal therapy triggered by NIR light, the biomimetic periosteum could promote bone regeneration at both the chemical and physical levels. This 3D-printed bilayer hydrogel can provide a promising strategy for preparing advanced tissue-engineered periosteum with excellent physical and bone regeneration properties.

理想的仿生骨膜应具有良好的生物相容性，以促进破骨细胞粘附并改善骨结合，这对促进骨再生意义重大。本研究采用 SLA-3D 打印技术制备了一种仿生人工骨膜，由聚（乙二醇）二丙烯酸酯（PEGDA）/壳聚糖/磷酸三钙（TCP）纤维层和明胶甲基丙烯酰（GelMA）/钼酸铵（Mo）骨膜层组成。仿生骨膜的两面都具有不同的表面特征。其中，纤维层具有较高的机械性能和较低的孔隙率，有利于防止肌肉组织的牵拉和软组织的侵入。骨膜层具有多孔结构和生物活性因子，能有效促进前成骨细胞的成骨分化。结合近红外光引发的温和光热疗法，仿生骨膜可在化学和物理层面促进骨再生。这种三维打印双层水凝胶为制备具有优异物理和骨再生特性的先进组织工程骨膜提供了一种前景广阔的策略。

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引用次数: 0

Folic acid-based hydrogels co-assembled with protocatechuic acid for enhanced treatment of inflammatory bowel disease 叶酸基水凝胶与原儿茶酸共同组装，用于加强对炎症性肠病的治疗。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114367

Ximei Ye , Tao Chen , Yinan Du , Runan Zhao , Lihang Chen , Di Wu , Jiangning Hu

Inflammatory bowel disease (IBD) presents a significant therapeutic challenge due to the need for oral drug delivery systems that withstand acidic environment of stomach while effectively targeting intestinal inflammation. To address this issue, we created a novel hydrogel system based on a folic acid (FA)-dopamine (DA) conjugate, co-assembled with protocatechuic acid (PCA), to form F-DP hydrogels. These hydrogels demonstrated robust anti-gastric acid, mucosal adhesive, and injectable properties, enhancing their efficacy for targeted delivery. In DSS-induced colitis mouse models, treatment with F-DP hydrogels resulted in significant therapeutic improvements, including increased body weight, reduced disease activity index (DAI), and maintained colon length. Biochemical assays revealed that F-DP hydrogels significantly enhanced antioxidant enzyme activities (GSH and SOD) and reduced oxidative stress markers (NO and MDA). Histological assessments confirmed effective repair of the colonic mucosal barrier, restoration of tight junction protein ZO-1, and reduction of inflammatory lesions. Furthermore, immunofluorescence staining indicated that F-DP hydrogels facilitated macrophages polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype, thereby reducing inflammation and promoting tissue repair. Our study demonstrates that F-DP hydrogels show significant potential for improving IBD treatment through enhanced gastric resistance, intestinal adhesion, and synergistic anti-inflammatory effects, warranting further investigation for clinical applications.

炎症性肠病（IBD）给治疗带来了巨大挑战，因为需要既能承受胃酸环境又能有效针对肠道炎症的口服给药系统。为解决这一问题，我们创建了一种新型水凝胶系统，该系统基于叶酸（FA）-多巴胺（DA）共轭物，与原儿茶酸（PCA）共同组装形成 F-DP 水凝胶。这些水凝胶具有很强的抗胃酸、粘膜粘附性和可注射性，从而提高了其靶向递送的功效。在 DSS 诱导的结肠炎小鼠模型中，使用 F-DP 水凝胶治疗可显著改善治疗效果，包括增加体重、降低疾病活动指数（DAI）和保持结肠长度。生化分析表明，F-DP 水凝胶显著提高了抗氧化酶活性（GSH 和 SOD），降低了氧化应激指标（NO 和 MDA）。组织学评估证实了结肠粘膜屏障的有效修复、紧密连接蛋白 ZO-1 的恢复以及炎症病变的减少。此外，免疫荧光染色表明，F-DP 水凝胶可促进巨噬细胞从促炎的 M1 表型极化为抗炎的 M2 表型，从而减轻炎症并促进组织修复。我们的研究表明，F-DP 水凝胶通过增强胃抵抗力、肠粘附力和协同抗炎作用，在改善 IBD 治疗方面显示出巨大的潜力，值得进一步研究其临床应用。

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引用次数: 0

Prussian blue nanocages as efficient radical scavengers and photothermal agents for reducing amyloid-beta induced neurotoxicity 普鲁士蓝纳米笼作为高效自由基清除剂和光热剂，可降低淀粉样蛋白-β诱导的神经毒性。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114369

Ting Wu , Xining Zhang , Shuangfei Cai , Wei Zhang , Rong Yang

The unusual accumulation of amyloid-beta 1–42 (Aβ₄₂) is an essential pathological feature of Alzheimer’s disease (AD), and development of Aβ₄₂ nanomodulators offers a potentially therapeutic approach to AD. Here, we report facile synthesis of the hollow mesocrystalline Prussian blue nanocages (HMPBs), which serve as versatile Aβ₄₂ modulators. Due to the hollow nanostructures and large specific surface area, they can effectively inhibit Aβ₄₂ aggregation by adsorption. They also exhibit robust near-infrared (NIR) photothermal effect for light-to-heat transition, which promotes the depolymerization of Aβ₄₂ fibers. Besides, they display ROS quenching ability to scavenge hydroxyl radicals (•OH) caused by Aβ₄₂ fibers, alleviate cellular oxidative stress, and improve cell survival. This work provides a new kind of Prussian blue nanomaterial for multimodal Aβ modulation.

淀粉样蛋白-β 1-42 (Aβ42)的异常积累是阿尔茨海默病（AD）的一个基本病理特征，Aβ42纳米调节剂的开发提供了一种潜在的AD治疗方法。在这里，我们报告了中空介晶普鲁士蓝纳米笼（HMPBs）的简易合成，它可作为多功能的 Aβ42 调节剂。由于中空的纳米结构和较大的比表面积，它们可以通过吸附作用有效抑制 Aβ42 的聚集。它们还具有强大的近红外（NIR）光热效应，可实现光热转换，促进 Aβ42 纤维的解聚。此外，它们还具有淬灭 ROS 的能力，能清除 Aβ42 纤维产生的羟自由基（-OH），减轻细胞氧化应激，提高细胞存活率。这项工作为多模式 Aβ 调节提供了一种新型普鲁士蓝纳米材料。

引用次数: 0

Development and characterization of PVA-zein/α-tocopherol nonwoven mats for functional wound dressing applications 用于功能性伤口敷料的 PVA-zein/α-tocopherol 非织造布垫的开发与表征。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-07 DOI: 10.1016/j.colsurfb.2024.114355

Debela T. Tadele , Dency David , Evelyn Yim , Tizazu H. Mekonnen

Wound healing poses significant clinical challenges due to issues like bacterial infections, oxidative stress, and the need for sustained therapeutic delivery. This study aimed to develop and characterize biocompatible nonwoven fibrous mats composed of poly(vinyl alcohol) (PVA) and zein encapsulating α-tocopherol for wound dressing applications. α-Tocopherol was nano-encapsulated in zein proteins using an antisolvent co-precipitation method, followed by its dispersion in PVA solutions. The resulting composition was then processed using a novel, scalable, and inexpensive solution blow spinning (SBS) process that offers higher throughputs to generate non-woven mats. The resulting fibers in the non-woven mats, ranging in diameter from 350 nm to 796 nm, demonstrate uniform morphology, as confirmed by scanning electron microscopy. Fourier transform infrared (FTIR) spectroscopy validated the successful incorporation of α-tocopherol without altering the chemical structure of the PVA-zein matrix. Rheological assessments revealed Newtonian behavior and a decrease in viscosity with higher tocopherol content, enhancing the processability of the mats. Mechanical testing showed that increasing tocopherol content improved tensile strength, elongation, and Young's modulus. The mats exhibited a biphasic release profile with an initial burst and sustained α-tocopherol release over 24 h, fitting the Korsmeyer-Peppas model and hence indicating a diffusion-controlled mechanism. Cytotoxicity assays confirmed high cell viability (>90 %) and enhanced cell spreading, underscoring their biocompatibility. These findings suggest that PVA-zein/tocopherol fiber mats are promising candidates for functional wound dressing materials, offering sustained antioxidant activity and a favorable wound healing environment. Future work will focus on optimizing fiber composition for antimicrobial properties and conducting in vivo studies to validate their clinical efficacy.

由于细菌感染、氧化应激等问题以及持续给药的需要，伤口愈合给临床带来了巨大挑战。本研究旨在开发和表征由聚（乙烯醇）（PVA）和玉米蛋白组成的、封装有α-生育酚的生物相容性非织造纤维垫，用于伤口敷料。采用抗溶剂共沉淀法将α-生育酚纳米封装在玉米蛋白中，然后将其分散在 PVA 溶液中。然后使用一种新颖、可扩展且成本低廉的溶液吹塑纺丝（SBS）工艺对所得到的成分进行加工，这种工艺能提供更高的产量来生成无纺布垫。经扫描电子显微镜确认，无纺布垫中的纤维直径从 350 纳米到 796 纳米不等，形态均匀。傅立叶变换红外光谱（FTIR）验证了α-生育酚的成功加入，而不会改变 PVA-zein基质的化学结构。流变学评估显示，随着生育酚含量的增加，α-生育酚具有牛顿特性，粘度降低，从而提高了毡垫的加工性能。机械测试表明，生育酚含量的增加提高了拉伸强度、伸长率和杨氏模量。这种垫子呈现出双相释放曲线，即初始爆发和 24 小时内持续释放 α-生育酚，符合 Korsmeyer-Peppas 模型，因此表明这是一种扩散控制机制。细胞毒性试验证实了其较高的细胞存活率（大于 90%）和较强的细胞铺展性，突出了其生物相容性。这些研究结果表明，PVA-zein/生育酚纤维毡具有持续的抗氧化活性和良好的伤口愈合环境，有望成为功能性伤口敷料的候选材料。今后的工作重点将是优化纤维成分以获得抗菌特性，并开展体内研究以验证其临床疗效。

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引用次数: 0

Ginger vesicle as a nanocarrier to deliver 10-hydroxycamptothecin. 生姜囊泡作为一种纳米载体来递送 10-羟基喜树碱。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-06 DOI: 10.1016/j.colsurfb.2024.114357

Zhongkai Liu, Jin Huang, Mengqi Liu, Liying Cui, Xiaoyu Li, Qi Meng, Xiaoshuai Wang, Shengkai Liu, Jinsong Peng, Zhiguo Liu

In this study, we developed the ginger vesicles as nanocarrier for the targeted delivery of 10-hydroxy-camptothecin (HCPT), aiming to improve its therapeutic efficacy while minimizing the systemic toxicity. Ginger vesicles exhibit a wide spectrum of biological activities and excellent biocompatibility, rendering them as the promising nanocarriers candidates for anticancer drug delivery. The ginger vesicles with an average diameter of 86.83 nm were successfully prepared by utilizing a gradient centrifugation method. The loading conditions for HCPT into the ginger vesicles were optimized through the addition of an appropriate amount of Ca²⁺. The loading efficiency, size distribution, stability, and cytotoxicity profile of the ginger vesicles were comprehensively characterized using UV spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and cytotoxicity experiments. Furthermore, in vitro cytotoxicity studies confirmed that ginger vesicles loaded with HCPT exhibited high inhibitory activity against tumor cells as evidenced by fluorescence imaging and flow cytometry analysis. Most importantly, in vivo antitumor assay demonstrated that the ginger vesicles loaded with HCPT displayed remarkable inhibitory effects on tumor growth. In summary, our results demonstrated the potential application of the ginger vesicles as ideal nanocarriers for delivering HCPT.

在这项研究中，我们开发了生姜囊泡作为纳米载体，用于靶向递送 10-羟基喜树碱（HCPT），旨在提高其疗效的同时最大限度地降低其全身毒性。生姜囊泡具有广泛的生物活性和良好的生物相容性，使其成为有望用于抗癌药物递送的纳米载体。利用梯度离心法成功制备了平均直径为 86.83 nm 的生姜囊泡。通过添加适量的 Ca2+ 优化了 HCPT 在生姜囊泡中的负载条件。利用紫外光谱、透射电子显微镜（TEM）、动态光散射（DLS）和细胞毒性实验对生姜囊泡的装载效率、粒度分布、稳定性和细胞毒性概况进行了全面的表征。此外，体外细胞毒性研究证实，通过荧光成像和流式细胞仪分析，负载 HCPT 的生姜囊泡对肿瘤细胞具有很高的抑制活性。最重要的是，体内抗肿瘤试验表明，负载 HCPT 的生姜囊泡对肿瘤生长有显著的抑制作用。总之，我们的研究结果证明了生姜囊泡作为理想的纳米载体来递送 HCPT 的潜在应用价值。

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引用次数: 0

Fabrication of functional interface on magnetic beads via various amino acids and their application in chemiluminescent immunoassay as carrier. 通过各种氨基酸在磁珠上制作功能界面，并将其作为载体应用于化学发光免疫测定。

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2024-11-06 DOI: 10.1016/j.colsurfb.2024.114364

Shihong Zhu, Daohang Du, Zhimin Zhao, Xianfeng Chu, Daoxiang Su, Shuli Yu, Ting Tao, Yong Jiang, Zhifei Wang

Magnetic polymer microspheres with superparamagnetism, high specificity, and monodispersity play a crucial role in the field of in vitro diagnostics. However, the surface modification process of magnetic beads is often complex, and it remains a significant challenge to prepare high-performance magnetic beads easily. To overcome these drawbacks, herein we fabricated functional interface on magnetic bead with the various amino acid via the ring-opening reaction of amino acids with epoxy groups, with attempt to produce carboxylated magnetic beads (MPS-GA) in a convenient way. Results indicate that when compared to other amino acids, the phenylalanine magnetic beads (MPS-GA1) developed in this study exhibit strong adsorption for mouse immunoglobulin (IgG), streptavidin (SA), and protamine (PA), with an IgG adsorption capacity of 53.5 μg/mg and a coupling capacity of 52.5 μg/mg. It is found that electrostatic forces and hydrophobic interactions are key factors influencing biomolecular interactions. Additionally, these magnetic beads can generate strong chemiluminescent signals, significantly reducing background levels by up to 99.7 %. Therefore, the magnetic beads proposed in this paper can serve as carriers for chemiluminescent immunoassay (CLIA), providing new insights into the synthesis of high-quality magnetic bead.

具有超顺磁性、高特异性和单分散性的磁性聚合物微球在体外诊断领域发挥着至关重要的作用。然而，磁珠的表面改性过程通常比较复杂，要轻松制备高性能磁珠仍是一项重大挑战。为了克服这些弊端，我们通过氨基酸与环氧基团的开环反应，在磁珠上形成了各种氨基酸的功能界面，试图以简便的方法制备羧基化磁珠（MPS-GA）。结果表明，与其他氨基酸相比，本研究开发的苯丙氨酸磁珠（MPS-GA1）对小鼠免疫球蛋白（IgG）、链霉亲和素（SA）和原丙胺（PA）具有很强的吸附能力，其中 IgG 的吸附能力为 53.5 μg/mg，偶联能力为 52.5 μg/mg。研究发现，静电力和疏水相互作用是影响生物分子相互作用的关键因素。此外，这些磁珠还能产生强烈的化学发光信号，显著降低背景水平达 99.7%。因此，本文提出的磁珠可作为化学发光免疫测定（CLIA）的载体，为高质量磁珠的合成提供了新的见解。

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