M. Yamada, T. Tegoshi, R. Uchikawa, S. Matsuda, M. Nishida, N. Arizono
IgE-inducing potential of excretory/secretory products (ES) of the nematode Nippostrongylus brasiliensis was examined in mice immunized with ES without use of adjuvant. Repeated i.p. injections or continuous i.p. or s.c. infusion of ES induced significant increases in total serum IgE. Continuous i.p. or s.c. infusion of ES also induced ES-specific IgE antibody, while repeated i.p. injections did not. IL-4 production was increased by ex vivo stimulation with ES of mesenteric lymph node cells from repeatedly immunized mice.On the other hand, continuous intraduodenal infusion of ES induced neither total nor specific IgE responses. These results suggest that continuously secreted ES from the nematode are relevant for the up-regulation of IL-4 and IgE responses, although additional factor (s) may be necessary for the uptake of ES across the intestinal mucosal barrier.
{"title":"INDUCTION OF IGE ANTIBODY RESPONSE IN MICE BY CONTINUOUS ADMINISTRATION OF EXCRETORY/SECRETORY PRODUCTS OF THE NEMATODE NIPPOSTRONGYLUS BRASILIENSIS","authors":"M. Yamada, T. Tegoshi, R. Uchikawa, S. Matsuda, M. Nishida, N. Arizono","doi":"10.2149/TMH1973.29.361","DOIUrl":"https://doi.org/10.2149/TMH1973.29.361","url":null,"abstract":"IgE-inducing potential of excretory/secretory products (ES) of the nematode Nippostrongylus brasiliensis was examined in mice immunized with ES without use of adjuvant. Repeated i.p. injections or continuous i.p. or s.c. infusion of ES induced significant increases in total serum IgE. Continuous i.p. or s.c. infusion of ES also induced ES-specific IgE antibody, while repeated i.p. injections did not. IL-4 production was increased by ex vivo stimulation with ES of mesenteric lymph node cells from repeatedly immunized mice.On the other hand, continuous intraduodenal infusion of ES induced neither total nor specific IgE responses. These results suggest that continuously secreted ES from the nematode are relevant for the up-regulation of IL-4 and IgE responses, although additional factor (s) may be necessary for the uptake of ES across the intestinal mucosal barrier.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"62-63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131827743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Simulium weji sp. nov. is described based on the reared females, reared males, pupae and mature larvae collected from Tharn Thong Waterfall in Lampang Province, Thailand. This new species is assigned to the tuberosum species-group within the subgenus Simulium s. str., and seems to be closely related to S. rufibasis Brunetti and four other allied species, reported from India and Thailand, by having a pair of hair clusters on the ventral surface of the seventh abdominal segment of the female, which are, though, much longer and fewer in this new species. This new species seems to be also related to S. nigrifacies Datta from India and S. puliense Takaoka from Taiwan, by having the similar arrangement of six short pupal gill filaments, but is easily differentiated from these known species by the small cone-shaped tubercles on the head and thoracic integuments.
根据在泰国南邦省Tharn Thong瀑布采集的雌性、雄性、蛹和成熟幼虫的资料，描述了维姬小蝇。这个新种被归为Simulium s. str.亚属的tuberosum种属，似乎与印度和泰国报道的s. rufibasis Brunetti和其他四个亲缘种密切相关，因为在雌性腹部第七节的腹面有一对毛簇，尽管在这个新种中，这些毛簇更长更少。该新种与印度的S. nigrifacies Datta和台湾的S. puliense Takaoka似乎也有亲缘关系，因为它们有相似的6条短蛹鳃丝的排列，但由于头和胸被上的小圆锥形结节，很容易与这些已知物种区分开来。
{"title":"SIMULIUM (SIMULIUM) WEJI SP. NOV. (DIPTERA : SIMULIIDAE) FROM THAILAND","authors":"Hiroyuki Takaoka","doi":"10.2149/TMH1973.29.349","DOIUrl":"https://doi.org/10.2149/TMH1973.29.349","url":null,"abstract":"Simulium weji sp. nov. is described based on the reared females, reared males, pupae and mature larvae collected from Tharn Thong Waterfall in Lampang Province, Thailand. This new species is assigned to the tuberosum species-group within the subgenus Simulium s. str., and seems to be closely related to S. rufibasis Brunetti and four other allied species, reported from India and Thailand, by having a pair of hair clusters on the ventral surface of the seventh abdominal segment of the female, which are, though, much longer and fewer in this new species. This new species seems to be also related to S. nigrifacies Datta from India and S. puliense Takaoka from Taiwan, by having the similar arrangement of six short pupal gill filaments, but is easily differentiated from these known species by the small cone-shaped tubercles on the head and thoracic integuments.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128516467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EPIDEMIOLOGY OF MALARIA AND A NEW PROPOSAL OF THE CONTROL PRINCIPLE BASED ON THE EVOLUTIONARY ECOLOGY OF ITS AGENTS AND VECTORS","authors":"T. Ikemoto, K. Takai","doi":"10.2149/TMH1973.29.273","DOIUrl":"https://doi.org/10.2149/TMH1973.29.273","url":null,"abstract":"","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131865314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Topics of Infectious Diseases Presented at the 7th Conference of the International Society of Travel Medicine","authors":"M. Kimura, H. Kikuchi","doi":"10.2149/TMH1973.29.281","DOIUrl":"https://doi.org/10.2149/TMH1973.29.281","url":null,"abstract":"","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126541837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Yamada, R. Yamada, Moazzem Hossain, S. Tsukidate, K. Fujita
Experimental Acanthamoeba keratitis was induced in 24 female Dutch rabbits to examine the clinical effects of infecton in the eye and to study the usefulness of a new histopathological technique for evaluating rabbit models of this infection. One eye of each animal in group A or B received an instillation of 1.3 X 104 (group A) or 1.3 X 105 (group B) amoeba cysts/eye; phosphate-buffered saline (PBS) solution was instilled into the contralateral eye as the control; animals in groups C and D received intrastromal injections of 5.0 X 103 (group C) or 1.5 X 104 (group D) cysts/eye in one eye and injection of an equal volume of PBS in the contralateral eye. Animals were observed daily for 5 to 84 days. Two rabbits in group D were killed on day 5 and enucleated eyes were embedded in paraffin and stained with hematoxylin-eosin or iodine-potassium iodide. In groups A and B, clinical signs of corneal injury disappeared by 4 hours after inoculation and signs of infection disappeared by day 2. In contrast, all eyes that had been injected with Acanthamoeba (groups C and D) developed severe keratitis, including keratoneuritis and corneal ulcer, followed by neovascularization or corneal perforation. Histologic examination showed infiltrates of leukocytes, lymphocytes, eosinophils, plasmacytes and spindle-shaped cells. The most extensive cell infiltration, and also exocytosis, liquefaction degeneration and intrastromal trophozoites, were seen in the limbic conjunctiva and palpebral conjunctiva. In addition, there was evidence of migration of inflammatory cells to the ciliary body and intravitreal space. This study showed that injection of Acanthamoeba into corneal stroma causes severe infection of the cornea and other eye tissues and that iodine-potassium iodide staining of paraffin embedded specimens is useful to detect Acanthamoeba trophozoites and cysts.
采用实验性棘阿米巴角膜炎诱导24只雌性荷兰兔，观察眼内感染的临床效果，并探讨一种新的组织病理学技术评价兔感染模型的有效性。A、B组每只动物一只眼滴注1.3 × 104 (A组)或1.3 × 105 (B组)阿米巴囊肿/眼;对侧眼内灌注磷酸盐缓冲盐水(PBS)溶液作为对照;C组和D组单眼眼内注射5.0 X 103 (C组)或1.5 X 104 (D组)囊/眼，对侧眼注射等量PBS。每天观察5 ~ 84 d。D组于第5天处死2只，取去核眼石蜡包埋，苏木精-伊红或碘-碘化钾染色。A、B组接种后4 h角膜损伤临床症状消失，第2天感染症状消失。相比之下，所有注射棘阿米巴的眼睛(C组和D组)都出现了严重的角膜炎，包括角膜神经炎和角膜溃疡，随后出现新生血管或角膜穿孔。组织学检查显示白细胞、淋巴细胞、嗜酸性粒细胞、浆细胞及梭形细胞浸润。边缘结膜和眼睑结膜以最广泛的细胞浸润、胞吐、液化变性和滋养体为主。此外，有证据表明炎症细胞迁移到睫状体和玻璃体腔。本研究表明，角膜基质注射棘阿米巴可引起角膜及其他眼部组织的严重感染，石蜡包埋标本碘化钾染色可用于棘阿米巴滋养体和囊肿的检测。
{"title":"CLINICAL AND PATHOLOGICAL FEATURES OF EXPERIMENTAL ACANTHAMOEBA KERATITIS IN RABBITS","authors":"S. Yamada, R. Yamada, Moazzem Hossain, S. Tsukidate, K. Fujita","doi":"10.2149/TMH1973.29.235","DOIUrl":"https://doi.org/10.2149/TMH1973.29.235","url":null,"abstract":"Experimental Acanthamoeba keratitis was induced in 24 female Dutch rabbits to examine the clinical effects of infecton in the eye and to study the usefulness of a new histopathological technique for evaluating rabbit models of this infection. One eye of each animal in group A or B received an instillation of 1.3 X 104 (group A) or 1.3 X 105 (group B) amoeba cysts/eye; phosphate-buffered saline (PBS) solution was instilled into the contralateral eye as the control; animals in groups C and D received intrastromal injections of 5.0 X 103 (group C) or 1.5 X 104 (group D) cysts/eye in one eye and injection of an equal volume of PBS in the contralateral eye. Animals were observed daily for 5 to 84 days. Two rabbits in group D were killed on day 5 and enucleated eyes were embedded in paraffin and stained with hematoxylin-eosin or iodine-potassium iodide. In groups A and B, clinical signs of corneal injury disappeared by 4 hours after inoculation and signs of infection disappeared by day 2. In contrast, all eyes that had been injected with Acanthamoeba (groups C and D) developed severe keratitis, including keratoneuritis and corneal ulcer, followed by neovascularization or corneal perforation. Histologic examination showed infiltrates of leukocytes, lymphocytes, eosinophils, plasmacytes and spindle-shaped cells. The most extensive cell infiltration, and also exocytosis, liquefaction degeneration and intrastromal trophozoites, were seen in the limbic conjunctiva and palpebral conjunctiva. In addition, there was evidence of migration of inflammatory cells to the ciliary body and intravitreal space. This study showed that injection of Acanthamoeba into corneal stroma causes severe infection of the cornea and other eye tissues and that iodine-potassium iodide staining of paraffin embedded specimens is useful to detect Acanthamoeba trophozoites and cysts.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114929305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Otsuka, C. Aoki, Katsumi Saito, U. Hadi, Hiroshi Suzuki, H. Takaoka
Nucleotide sequences of a subregion of the mitochondrial 16S ribosomal RNA gene of 10 species of a blackfly subgenus Simulium (Nevermannia), which include four species of feuerborni species-group, two species of ruficorne species-group, three species of vernum species-group and an ungrouped species (S. konoi), were de- termined. Phylogenetic analyses of the sequences of the Nevermannia species and other species of related subgen- era of Simulium s.l. showed that the feuerborni and vernum species-groups were closely related, but the ruficorne species-group and S. konoi were not. Variations between the ruficorne species-group and other Nevermannia spe- cies were larger than those between Nevermannia species (excluding the ruficorne species-group) and other sub- genera species. These molecular data suggest that revision of the definition of the subgenus Nevermannia is needed.
测定了黑蝇亚属Simulium (Nevermannia) 10种(包括4种feuerborni种群、2种ruficorne种群、3种vernum种群和1种ungrouped species (S. konoi))线粒体16S核糖体RNA基因亚区核苷酸序列。系统发育分析表明，牛角蕨属(feuerborni)和牛角蕨属(vernum)种群亲缘关系较近，而牛角蕨属(ruficorne)种群与konoi属(S. konoi)亲缘关系不密切。金合欢种群与其他金合欢种群之间的差异大于金合欢种群(不含金合欢种群)与其他亚属种之间的差异。这些分子数据表明，需要修订梦幻草亚属的定义。
{"title":"PHYLOGENETIC ANALYSES OF A BLACKFLY SUBGENUS SIMULIUM (NEVERMANNIA) BASED ON MITOCHONDRIAL 16S RIBOSOMAL RNA GENE SEQUENCES","authors":"Y. Otsuka, C. Aoki, Katsumi Saito, U. Hadi, Hiroshi Suzuki, H. Takaoka","doi":"10.2149/TMH1973.29.261","DOIUrl":"https://doi.org/10.2149/TMH1973.29.261","url":null,"abstract":"Nucleotide sequences of a subregion of the mitochondrial 16S ribosomal RNA gene of 10 species of a blackfly subgenus Simulium (Nevermannia), which include four species of feuerborni species-group, two species of ruficorne species-group, three species of vernum species-group and an ungrouped species (S. konoi), were de- termined. Phylogenetic analyses of the sequences of the Nevermannia species and other species of related subgen- era of Simulium s.l. showed that the feuerborni and vernum species-groups were closely related, but the ruficorne species-group and S. konoi were not. Variations between the ruficorne species-group and other Nevermannia spe- cies were larger than those between Nevermannia species (excluding the ruficorne species-group) and other sub- genera species. These molecular data suggest that revision of the definition of the subgenus Nevermannia is needed.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128766517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two new black-fly species, Simulium (Gomphostilbia) guniki sp. nov. and S. (Nevermannnia) borneoense sp. nov., are described from adult flies emerged from pupae, collected from Sabah in Malaysia. S. guniki is assigned in the ceylonicum species-group, and is characterized by the darkened legs in the adults of both sexes, eight pupal gill filaments almost sessile, and the small postgenal cleft of the larva. S. borneoense, which is assigned to the feuerborni species-group, is most distinctive by having the four pupal gill filaments per side (in place of usual six filaments). In addition, three species of Simulium (Gomphostilbia) , i.e., S. dentistylum, S. parahiyangum, and S. sheilae, are newly recorded from Sabah.
{"title":"Two New and Three Newly Recorded Species of Black Flies (Diptera: Simuliidae) from Sabah, Malaysia.","authors":"H. Takaoka","doi":"10.2149/TMH1973.29.221","DOIUrl":"https://doi.org/10.2149/TMH1973.29.221","url":null,"abstract":"Two new black-fly species, Simulium (Gomphostilbia) guniki sp. nov. and S. (Nevermannnia) borneoense sp. nov., are described from adult flies emerged from pupae, collected from Sabah in Malaysia. S. guniki is assigned in the ceylonicum species-group, and is characterized by the darkened legs in the adults of both sexes, eight pupal gill filaments almost sessile, and the small postgenal cleft of the larva. S. borneoense, which is assigned to the feuerborni species-group, is most distinctive by having the four pupal gill filaments per side (in place of usual six filaments). In addition, three species of Simulium (Gomphostilbia) , i.e., S. dentistylum, S. parahiyangum, and S. sheilae, are newly recorded from Sabah.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116358848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper showed the trend in the number of paper published on emerging and reemerging diseases by using the list of the Medline, which is one of the most valuable databases in the clinical and biological medicine. The number of research papers published on the emerging and reemerging infections in that database decreased in 1997, in comparison with those between 1994 and 1996, in spite of the fact that the total number of the paper pub- lished has been increasing year by year. There is also a report showing that the number of the published papers of noticeable infectious diseases such as AIDS, Hepatitis B, Gonorrhea, Pertussis and Tuberculosis declined by 15% or more in the USA from 1993 to 1995. It also became clear that USA is the highest in rank for the number of pa- pers published and it was well ahead of the other countries. However, as for Vibrio cholerae O139, Human T- lymphtropic Virus type 1 and Plague, a lot of papers have been published by responding countries like India, Japan and Russia, respectively. This result might be showing that research activity of each country for each disease re- lates to the extent of their concern over each disease.
{"title":"RESEARCH ACTIVITIES ON EMERGING AND REEMERGING DISEASES","authors":"Taro Yamamoto, M. Shimada, K. Moji, T. Mizota","doi":"10.2149/TMH1973.29.231","DOIUrl":"https://doi.org/10.2149/TMH1973.29.231","url":null,"abstract":"This paper showed the trend in the number of paper published on emerging and reemerging diseases by using the list of the Medline, which is one of the most valuable databases in the clinical and biological medicine. The number of research papers published on the emerging and reemerging infections in that database decreased in 1997, in comparison with those between 1994 and 1996, in spite of the fact that the total number of the paper pub- lished has been increasing year by year. There is also a report showing that the number of the published papers of noticeable infectious diseases such as AIDS, Hepatitis B, Gonorrhea, Pertussis and Tuberculosis declined by 15% or more in the USA from 1993 to 1995. It also became clear that USA is the highest in rank for the number of pa- pers published and it was well ahead of the other countries. However, as for Vibrio cholerae O139, Human T- lymphtropic Virus type 1 and Plague, a lot of papers have been published by responding countries like India, Japan and Russia, respectively. This result might be showing that research activity of each country for each disease re- lates to the extent of their concern over each disease.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127560295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two new black-fly species, Simulium (Gomphostilbia) lehi sp. nov. and S. (G.) sarawakense sp. nov., are described from adult flies reared from pupae, collected in Sarawak State, Malaysia. In addition, three known species of Simulium s.l., i.e., S. (G.) sheilae, S. (Nevermannia) aureohirtum, S. (Simulium) laterale, are newly recorded from Sarawak.
{"title":"DESCRIPTION OF TWO NEW SPECIES OF BLACK FLIES (DIPTERA : SIMULIIDAE) FROM SARAWAK, MALAYSIA","authors":"H. Takaoka","doi":"10.2149/TMH1973.29.243","DOIUrl":"https://doi.org/10.2149/TMH1973.29.243","url":null,"abstract":"Two new black-fly species, Simulium (Gomphostilbia) lehi sp. nov. and S. (G.) sarawakense sp. nov., are described from adult flies reared from pupae, collected in Sarawak State, Malaysia. In addition, three known species of Simulium s.l., i.e., S. (G.) sheilae, S. (Nevermannia) aureohirtum, S. (Simulium) laterale, are newly recorded from Sarawak.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122795369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ishak, I. Matsuse, K. Kamimura, T. Takegami, H. Funada
Five geographic strains of Aedes albopictus from Japan and Southeast Asia and three strains of Aedes aegypti from Indonesia and Pakistan were compared for their susceptibility to oral infection with the human virus, dengue-1 Mochizuki strain (isolated in 1943, Japan) and A88 strain (isolated in 1988, Indonesia). Female mosquitoes, aged 3-4 days, were infected with a virus-erythrocyte-sucrose suspension. After 14 days of incubation at 25-30°C, viral infection in mesenteron (midgut) or in head-thorax (salivary gland) of each individual mosquito was identified by the reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of dengue-1 virus envelope gene. Although the results of susceptibility varied in some extent in different strains of mosquitoes and viral strains, the oral susceptibility to both dengue-1 virus strains was not significantly different among dengue vector mosquitoes from Japan and Southeast Asia.
{"title":"COMPARATIVE ORAL SUSCEPTIBILITY OF DENGUE VECTOR MOSQUITOES FROM JAPAN AND SOUTHEAST ASIA TO TWO DENGUE-1 VIRUS STRAINS","authors":"H. Ishak, I. Matsuse, K. Kamimura, T. Takegami, H. Funada","doi":"10.2149/TMH1973.29.253","DOIUrl":"https://doi.org/10.2149/TMH1973.29.253","url":null,"abstract":"Five geographic strains of Aedes albopictus from Japan and Southeast Asia and three strains of Aedes aegypti from Indonesia and Pakistan were compared for their susceptibility to oral infection with the human virus, dengue-1 Mochizuki strain (isolated in 1943, Japan) and A88 strain (isolated in 1988, Indonesia). Female mosquitoes, aged 3-4 days, were infected with a virus-erythrocyte-sucrose suspension. After 14 days of incubation at 25-30°C, viral infection in mesenteron (midgut) or in head-thorax (salivary gland) of each individual mosquito was identified by the reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of dengue-1 virus envelope gene. Although the results of susceptibility varied in some extent in different strains of mosquitoes and viral strains, the oral susceptibility to both dengue-1 virus strains was not significantly different among dengue vector mosquitoes from Japan and Southeast Asia.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121495827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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