{"title":"MIM/TDR task force on malaria research capability strengthening in Africa.","authors":"K. Mendis, F. Zicker","doi":"10.2149/TMH1973.30.33","DOIUrl":"https://doi.org/10.2149/TMH1973.30.33","url":null,"abstract":"","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"230 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123005308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The President’s Lecture is held at each World Congress of the ISHR and, in non-Congress years, at the annual meeting of one of the 3 largest ISHR Sections on a rotating basis. This lecture is intended to be a high profile event and is scheduled as a keynote plenary lecture. The International Council selects the speaker. The topic of the lecture is in the field of molecular biology, genetics, genomics or proteomics, but the content should be chosen to be of broad interest to the cardiovascular community. The speaker is reimbursed for travel expenses, and receives a plaque and a $1,000 honorarium. A photograph and biosketch of the speaker is published in Heart News and Views, and is posted in the ISHR website.
{"title":"President's lecture","authors":"M. Aikawa","doi":"10.2149/TMH1973.30.32","DOIUrl":"https://doi.org/10.2149/TMH1973.30.32","url":null,"abstract":"The President’s Lecture is held at each World Congress of the ISHR and, in non-Congress years, at the annual meeting of one of the 3 largest ISHR Sections on a rotating basis. This lecture is intended to be a high profile event and is scheduled as a keynote plenary lecture. The International Council selects the speaker. The topic of the lecture is in the field of molecular biology, genetics, genomics or proteomics, but the content should be chosen to be of broad interest to the cardiovascular community. The speaker is reimbursed for travel expenses, and receives a plaque and a $1,000 honorarium. A photograph and biosketch of the speaker is published in Heart News and Views, and is posted in the ISHR website.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125912374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We produced antigenic peptide of Trichinella spiralis newborn larvae (NBL) which seemed to be a part (approximately one fourth) of myosin heavy chain, and some basic profiles were revealed. The cDNA library was constructed from NBL and immunoscreened with an antibody against the parasite. A clone, designated NBL21, was selected. It contained a cDNA transcript of 1656 by in length, which encoded 552-amino acids (64868 Da in the estimated molecular weight). The fusion protein encoded by the clone NBL21 was produced in an Escherichia coli expression system and affinity purified. NBL21 fusion proteins migrated at 64 kDa and reacted to T spiralis infected mouse sera and the antibody against NBL crude antigen. Antisera were developed against NBL21 fusion proteins, which reacted to a single band migrating at 200 kDa on Western blotting analysis of crude extracts from muscle larvae, and reacted to hypodermal muscles of T spiralis on immunohistochemical staining. The antigen was recognized by the mouse serum obtained from the early phase of infection, but the antigenicity was devoid of species specificity.
{"title":"AN ANTIGENIC PEPTIDE OF MYOSIN HEAVY CHAIN-LIKE PROTEIN FROM TRICHINELLA SPIRALIS","authors":"T. Nakada, I. Nagano, Zhiliang Wu, Y. Takahashi","doi":"10.2149/TMH1973.30.15","DOIUrl":"https://doi.org/10.2149/TMH1973.30.15","url":null,"abstract":"We produced antigenic peptide of Trichinella spiralis newborn larvae (NBL) which seemed to be a part (approximately one fourth) of myosin heavy chain, and some basic profiles were revealed. The cDNA library was constructed from NBL and immunoscreened with an antibody against the parasite. A clone, designated NBL21, was selected. It contained a cDNA transcript of 1656 by in length, which encoded 552-amino acids (64868 Da in the estimated molecular weight). The fusion protein encoded by the clone NBL21 was produced in an Escherichia coli expression system and affinity purified. NBL21 fusion proteins migrated at 64 kDa and reacted to T spiralis infected mouse sera and the antibody against NBL crude antigen. Antisera were developed against NBL21 fusion proteins, which reacted to a single band migrating at 200 kDa on Western blotting analysis of crude extracts from muscle larvae, and reacted to hypodermal muscles of T spiralis on immunohistochemical staining. The antigen was recognized by the mouse serum obtained from the early phase of infection, but the antigenicity was devoid of species specificity.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124698716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ishih, Mikako Sakai, H. Takezoe, K. Fujii, Masaki Sano, Norihiro Asanuma, T. Miyase, M. Terada
Hot-water extracts of 7 plants of the family Saxifragaceae; Hydrangea macrophylla var. Otaksa, H. macrophylla, H. macrophylla serrata var. acuminata, H. involucrata, H. hirta, H. paniculata, and Cardiandra alternifolia, were screened for antimalarial activity in ICR mice infected with Plasmodium yoelii 17XL. The leaf extract of H. macrophylla var. Otaksa or H. macrophylla had an antimalarial activity against rodent malaria, showing low parasitemia levels during administration. Following a transient recrudescence of malaria parasites in the bloodstream of treated mice, no parasites could be detected by a microscopic examination. Mice treated with the leaf extract of H. macrophylla var. Otaksa or H. macrophylla survived during the experiment, though mice in the non-treated control and other treated groups died with a gradual body weight loss from day 6 to day 10 post infection. Furthermore, the antimalarial activity of the hot-water extract of H. macrophylla leaf extract seemed higher than that of H. macrophylla var. Otaksa leaf extract in respect of the degree of suppression of parasite multiplication and of mouse body weight loss.
{"title":"A POTENT ANTIMALARIAL ACTIVITY OF HOT-WATER EXTRACTS OF PLANTS BELONGING TO THE FAMILY SAXIFRAGACEAE AGAINST PLASMODIUM YOELII 17XL IN ICR MICE","authors":"A. Ishih, Mikako Sakai, H. Takezoe, K. Fujii, Masaki Sano, Norihiro Asanuma, T. Miyase, M. Terada","doi":"10.2149/TMH1973.30.1","DOIUrl":"https://doi.org/10.2149/TMH1973.30.1","url":null,"abstract":"Hot-water extracts of 7 plants of the family Saxifragaceae; Hydrangea macrophylla var. Otaksa, H. macrophylla, H. macrophylla serrata var. acuminata, H. involucrata, H. hirta, H. paniculata, and Cardiandra alternifolia, were screened for antimalarial activity in ICR mice infected with Plasmodium yoelii 17XL. The leaf extract of H. macrophylla var. Otaksa or H. macrophylla had an antimalarial activity against rodent malaria, showing low parasitemia levels during administration. Following a transient recrudescence of malaria parasites in the bloodstream of treated mice, no parasites could be detected by a microscopic examination. Mice treated with the leaf extract of H. macrophylla var. Otaksa or H. macrophylla survived during the experiment, though mice in the non-treated control and other treated groups died with a gradual body weight loss from day 6 to day 10 post infection. Furthermore, the antimalarial activity of the hot-water extract of H. macrophylla leaf extract seemed higher than that of H. macrophylla var. Otaksa leaf extract in respect of the degree of suppression of parasite multiplication and of mouse body weight loss.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114932853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Description was given on the material and procedure of the nylon mesh filtration technique for quantitative detection of helminth eggs in the feces, and comparison was made between the filtration and the Kato-Katz techniques on the clearness and preservation of detected eggs and the efficiency of detection. In the filtration technique, fecal matter is filtered through nylon mesh to concentrate the eggs and the eggs are sealed in the mounting medium by melting the mesh fibers. This technique can detect all the helminth eggs including hook worm and small Clonorchis eggs that are difficult to be recognized by the Kato-Katz technique. The rate of coincidence of the positive and negative slides of Schistosoma eggs between the two techniques was 88.7%, being statistically high. The filtration technique can detect 2.4 times number of eggs that are collected by the Kato-Katz technique, and the sealed eggs are clear enough to identify and can be preserved for months. This filtration technique, simple and suitable for field use, can contribute to find the helminth infections and to evaluate the control work even in developing countries.
{"title":"NYLON MESH FILTRATION TECHNIQUE FOR QUANTITATIVE DETECTION OF HELMINTH EGGS","authors":"K. Makiya","doi":"10.2149/TMH1973.29.335","DOIUrl":"https://doi.org/10.2149/TMH1973.29.335","url":null,"abstract":"Description was given on the material and procedure of the nylon mesh filtration technique for quantitative detection of helminth eggs in the feces, and comparison was made between the filtration and the Kato-Katz techniques on the clearness and preservation of detected eggs and the efficiency of detection. In the filtration technique, fecal matter is filtered through nylon mesh to concentrate the eggs and the eggs are sealed in the mounting medium by melting the mesh fibers. This technique can detect all the helminth eggs including hook worm and small Clonorchis eggs that are difficult to be recognized by the Kato-Katz technique. The rate of coincidence of the positive and negative slides of Schistosoma eggs between the two techniques was 88.7%, being statistically high. The filtration technique can detect 2.4 times number of eggs that are collected by the Kato-Katz technique, and the sealed eggs are clear enough to identify and can be preserved for months. This filtration technique, simple and suitable for field use, can contribute to find the helminth infections and to evaluate the control work even in developing countries.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114174433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Haruki, F. Kobayashi, T. Fujino, T. Matsui, M. Tsuji
The AnaeroPack (AnP) system (Mitsui Gas Co., Japan) was produced for the purpose of culturing anaerobic bacteria. The idea using AnP Campylo® for cultivation of Plasmodium falciparum and its successful results were firstly reported by Haruki et al. (1997). In the present study, characterisation of 3 products of AnPs, i.e., AnP CO2® (at a gas phase of 5% CO2 and 15% O2), AnP Campylo® (10% CO2 and 10% O2) and AnP Plas® (5% CO2 and 5% O2), in an AnaeroPack Box Jar® (22×16×10.5 cm) was studied comparing with conditions in a gas incubator or gas chamber (4% CO2 and 3% O2) and in a candle jar (5% CO2 and 15% O2). The growth curves of malaria parasites showed no difference among these four conditions by incubation for 48 hr except for AnP Plas. The observed IC50 levels of chloroquine were varied much by the difference of AnPs, gas phases, the pressure in a Box Jar and free space inside. For stabilisation of IC50 testing, free space in a Box Jar should be kept equal and minimal by placing the maximal 5 test plates by adding dummy plates. By the stable results of IC50, gas phases can be divided into two groups. The one involves the gas incubator, AnP Plas® and AnP Campylo®, and the other does the candle jar and AnP CO2®. Results of chloroquine sensitivity test in the candle jar group showed more drug sensitive than in the gas incubator group. These two groups should be used properly by the purpose of field experiments. The AnP® system is proven to be a practical tool for malaria culture in the field for occupying only a small space in an incubator.
{"title":"Evaluation of AnaeroPack(AnP) Type as Tools of Plasmodium falciparum cultivation and Drug Sensitivity Tests.","authors":"K. Haruki, F. Kobayashi, T. Fujino, T. Matsui, M. Tsuji","doi":"10.2149/TMH1973.29.365","DOIUrl":"https://doi.org/10.2149/TMH1973.29.365","url":null,"abstract":"The AnaeroPack (AnP) system (Mitsui Gas Co., Japan) was produced for the purpose of culturing anaerobic bacteria. The idea using AnP Campylo® for cultivation of Plasmodium falciparum and its successful results were firstly reported by Haruki et al. (1997). In the present study, characterisation of 3 products of AnPs, i.e., AnP CO2® (at a gas phase of 5% CO2 and 15% O2), AnP Campylo® (10% CO2 and 10% O2) and AnP Plas® (5% CO2 and 5% O2), in an AnaeroPack Box Jar® (22×16×10.5 cm) was studied comparing with conditions in a gas incubator or gas chamber (4% CO2 and 3% O2) and in a candle jar (5% CO2 and 15% O2). The growth curves of malaria parasites showed no difference among these four conditions by incubation for 48 hr except for AnP Plas. The observed IC50 levels of chloroquine were varied much by the difference of AnPs, gas phases, the pressure in a Box Jar and free space inside. For stabilisation of IC50 testing, free space in a Box Jar should be kept equal and minimal by placing the maximal 5 test plates by adding dummy plates. By the stable results of IC50, gas phases can be divided into two groups. The one involves the gas incubator, AnP Plas® and AnP Campylo®, and the other does the candle jar and AnP CO2®. Results of chloroquine sensitivity test in the candle jar group showed more drug sensitive than in the gas incubator group. These two groups should be used properly by the purpose of field experiments. The AnP® system is proven to be a practical tool for malaria culture in the field for occupying only a small space in an incubator.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122052141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The mechanisms of antigen-uptake at the host mucosal barrier during infection with lumen-dwelling intestinal parasites have not been well elucidated. In this study, we investigated immunolocalization of parasite excretory/secretory (ES) products in the small intestinal epithelium of rats infected with Nippostrongylus brasiliensis. Immunoelectronmicroscopy revealed intense ES immunoreactivity in the absorptive cell cytoplasm in association with lysosome-associated membrane glycoprotein (LAMP-1), and in the paracellular spaces. These results indicated that nematode antigen could be absorbed by two pathways : absorption by endocytosis and transport to lysosomes, and absorption through the paracellular pathway, suggesting altered mucosal barrier function.
{"title":"MECHANISMS OF PARASITE ANTIGEN UPTAKE FROM THE INTESTINE OF RATS INFECTED WITH THE NEMATODE NIPPOSTRONGYLUS BRASILIENSIS","authors":"M. Yamada, R. Uchikawa, S. Matsuda, N. Arizono","doi":"10.2149/TMH1973.29.355","DOIUrl":"https://doi.org/10.2149/TMH1973.29.355","url":null,"abstract":"The mechanisms of antigen-uptake at the host mucosal barrier during infection with lumen-dwelling intestinal parasites have not been well elucidated. In this study, we investigated immunolocalization of parasite excretory/secretory (ES) products in the small intestinal epithelium of rats infected with Nippostrongylus brasiliensis. Immunoelectronmicroscopy revealed intense ES immunoreactivity in the absorptive cell cytoplasm in association with lysosome-associated membrane glycoprotein (LAMP-1), and in the paracellular spaces. These results indicated that nematode antigen could be absorbed by two pathways : absorption by endocytosis and transport to lysosomes, and absorption through the paracellular pathway, suggesting altered mucosal barrier function.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130227672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sugiyama, T. Shibahara, P. Ketudat, S. Thaithong, K. Kawashima
We examined specimens of adult Paragonimus westermani collected from Thai leopards by Daengsvang et al. in 1964. The two flukes examined had a six-lobed ovary each and singly-spaced cuticular spines. The seminal receptacle and seminal vesicle were filled with spermatozoa. Based on morphological features, these flukes were identified as the diploid type of P. westermani. The testis branched out into six lobes on the ovarian side and into five on the opposite side in both specimens. Abnormal branching in the testes, that is, separation of one lobe from the others and its direct connection to the was deferens, which was found in adult P westermani from the Philippines, was undetectable.
{"title":"MORPHOLOGICAL RE-EXAMINATION OF PARAGONIMUS WESTERMANI DESCRIBED BY DAENGSVANG AND OTHERS IN 1964","authors":"H. Sugiyama, T. Shibahara, P. Ketudat, S. Thaithong, K. Kawashima","doi":"10.2149/TMH1973.29.371","DOIUrl":"https://doi.org/10.2149/TMH1973.29.371","url":null,"abstract":"We examined specimens of adult Paragonimus westermani collected from Thai leopards by Daengsvang et al. in 1964. The two flukes examined had a six-lobed ovary each and singly-spaced cuticular spines. The seminal receptacle and seminal vesicle were filled with spermatozoa. Based on morphological features, these flukes were identified as the diploid type of P. westermani. The testis branched out into six lobes on the ovarian side and into five on the opposite side in both specimens. Abnormal branching in the testes, that is, separation of one lobe from the others and its direct connection to the was deferens, which was found in adult P westermani from the Philippines, was undetectable.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126964075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Q. Lin, K. Katakura, M. Oue, S. Kano, Mamoru Suzuki
Tetracyclines are used for prophylaxis of malaria and treatment of drug-resistant falciparum malaria because of their safe drug action. We re-evaluated effects of three tetracyclines against drug-resistant Plasmodium falciparum in vitro. Minocycline was approximately 4 times and twice more potent in inhibiting the in vitro growth of falciparum parasites than tetracycline and doxycycline, respectively. Compared with doxycycline, significant inhibitory effects of minocycline to chloroquine, pyrimethamine and mefloquine resistant P. falciparum strains were affirmed by the present in vitro study. By electron microscopy a number of electron dense vesicles with a single membrane bound were observed in the cytoplasm of minocycline-treated parasites, although no distinct structural alternations of mitochondria was noted. Minocycline may be a better therapeutic drug than doxycycline which is widely accented as the standard antimalarial tetracycline.
{"title":"Effects of Minocycline against Mefloquine-, Chloroquine- and Pyrimethamine-resistant Plasmodium falciparum in vitro.","authors":"Q. Lin, K. Katakura, M. Oue, S. Kano, Mamoru Suzuki","doi":"10.2149/TMH1973.29.343","DOIUrl":"https://doi.org/10.2149/TMH1973.29.343","url":null,"abstract":"Tetracyclines are used for prophylaxis of malaria and treatment of drug-resistant falciparum malaria because of their safe drug action. We re-evaluated effects of three tetracyclines against drug-resistant Plasmodium falciparum in vitro. Minocycline was approximately 4 times and twice more potent in inhibiting the in vitro growth of falciparum parasites than tetracycline and doxycycline, respectively. Compared with doxycycline, significant inhibitory effects of minocycline to chloroquine, pyrimethamine and mefloquine resistant P. falciparum strains were affirmed by the present in vitro study. By electron microscopy a number of electron dense vesicles with a single membrane bound were observed in the cytoplasm of minocycline-treated parasites, although no distinct structural alternations of mitochondria was noted. Minocycline may be a better therapeutic drug than doxycycline which is widely accented as the standard antimalarial tetracycline.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129130779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sugiyama, K. Lai, C. Ow-Yang, B. Blas, K. Kawashima
We studied the ultrastructure of excysted metacercariae of Paragonimus westermani from Malaysia and the Philippines using scanning electron microscopy. The body surface of the excysted metacercariae was covered with numerous single-pointed and thorn-like tegumentary spines. Dome-shaped papillae were evenly distributed over the whole body and situated circularly around the oral and ventral suckers. Between six to eight and seven to nine small dome-shaped papillae were observed around the ventral sucker in P. westermani from Malaysia and the Philippines, respectively. In general, the morphological features of the worms from Malaysia and the Philippines were identical.
{"title":"SURFACE ULTRASTRUCTURE OF NEWLY EXCYSTED METACERCARIAE OF PARAGONIMUS WESTERMANI FROM MALAYSIA AND THE PHILIPPINES","authors":"H. Sugiyama, K. Lai, C. Ow-Yang, B. Blas, K. Kawashima","doi":"10.2149/TMH1973.29.375","DOIUrl":"https://doi.org/10.2149/TMH1973.29.375","url":null,"abstract":"We studied the ultrastructure of excysted metacercariae of Paragonimus westermani from Malaysia and the Philippines using scanning electron microscopy. The body surface of the excysted metacercariae was covered with numerous single-pointed and thorn-like tegumentary spines. Dome-shaped papillae were evenly distributed over the whole body and situated circularly around the oral and ventral suckers. Between six to eight and seven to nine small dome-shaped papillae were observed around the ventral sucker in P. westermani from Malaysia and the Philippines, respectively. In general, the morphological features of the worms from Malaysia and the Philippines were identical.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"418 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117327638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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