Pub Date : 2009-03-04DOI: 10.17525/VRRJOURNAL.V14I1.25
A. D. Albuquerque, M. R. Coêlho, M. F. Lemos, A. Cruz, S. C. M. Braz, R. Moreira
Patients under hemodialysis are considered a high risk to acquire hepatitis B virus (HBV) infection. The aim of this study was to determine the serological and molecular profile and risk factors for HBV among hemodialysis patients in five clinics in Recife, Pernambuco, between August 2006 and August 2007. The study population (n = 781) was interviewed after signing a free and informed consent statement and the blood collected through the patient’s fistula prior to hemodialysis. Serum samples underwent ELISA to investigate total anti-HBc, HBsAg and anti-HBs. The samples that were HBsAg-positive were subjected to the PCR to investigate viral DNA. The DNA-positive samples were sequenced to identify the genotype. The information was stored and analyzed in Epi-Info 6.0. The seroprevalences of total anti-HBc, HBsAg and anti-HBs were 29.4% (229/781), 3.3% (26/781) and 66.2% (135/203), respectively. Among the 26 HBsAg-positive samples, 14 were positive for HBV DNA; genotypes A and F were found. The variables of sex, age, length of time on dialysis and number of transfusions showed statistically significant association with HBV, thus corroborating other Brazilian studies. Even with the implementation of HBV infection control measures and vaccinations, dialysis units still present widespread virus circulation. All units need to rigidly follow the universal precautions, so that the virus circulation in these places can increasingly be minimized. DOI: http://dx.doi.org/10.17525/vrrjournal.v14i1.25
{"title":"HEPATITIS B VIRUS INFECTION PROFILE IN DIFFERENT HEMODIALYSIS UNITS IN RECIFE, PERNAMBUCO, BRAZIL","authors":"A. D. Albuquerque, M. R. Coêlho, M. F. Lemos, A. Cruz, S. C. M. Braz, R. Moreira","doi":"10.17525/VRRJOURNAL.V14I1.25","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V14I1.25","url":null,"abstract":"Patients under hemodialysis are considered a high risk to acquire hepatitis B virus (HBV) infection. The aim of this study was to determine the serological and molecular profile and risk factors for HBV among hemodialysis patients in five clinics in Recife, Pernambuco, between August 2006 and August 2007. The study population (n = 781) was interviewed after signing a free and informed consent statement and the blood collected through the patient’s fistula prior to hemodialysis. Serum samples underwent ELISA to investigate total anti-HBc, HBsAg and anti-HBs. The samples that were HBsAg-positive were subjected to the PCR to investigate viral DNA. The DNA-positive samples were sequenced to identify the genotype. The information was stored and analyzed in Epi-Info 6.0. The seroprevalences of total anti-HBc, HBsAg and anti-HBs were 29.4% (229/781), 3.3% (26/781) and 66.2% (135/203), respectively. Among the 26 HBsAg-positive samples, 14 were positive for HBV DNA; genotypes A and F were found. The variables of sex, age, length of time on dialysis and number of transfusions showed statistically significant association with HBV, thus corroborating other Brazilian studies. Even with the implementation of HBV infection control measures and vaccinations, dialysis units still present widespread virus circulation. All units need to rigidly follow the universal precautions, so that the virus circulation in these places can increasingly be minimized. DOI: http://dx.doi.org/10.17525/vrrjournal.v14i1.25","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"14 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2009-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-20DOI: 10.17525/VRRJOURNAL.V13I1-2.15
L. Oliveira, Jacqueline G. S. Haimuri, C. Jacyntho, Tomas Z Barrese, G. Vasconcelos, S. Cavalcanti, M. Rosa
The present study aimed to investigate HPV prevalence associated with cervical cytology and to evaluate data from 140 HIV positive women routinely attended at an out-patient public gynecological service in Rio de Janeiro state. The CD4 cell counts and HIV-1 RNA levels were determined using standardized protocols. HPV status and HPV 16 detection were ascertained respectively by My09/11 consensus primers and type specific primers. Colpocytology was performed at the first or subsequent visit to the service. Among women, 98.6% of them were under antiviral treatment, 92.1% had over 200 CD4 cells/mm3, 62.9% had detectable HIV RNA, 60.7% were HPV infected and 21.2 % harbored the HPV type 16. Normal/inflammatory results were prevalent (57.1%) and 7.9% of the patients had HSIL. Studying demographic data and HIV markers that could affect HPV infection, we found that time elapsed since positive diagnosis of less than four years was a significant factor. First sexual intercourse beginning earlier than 17 years, people younger than 30 years and white ethnicity were also linked to HPV infection. Alcohol use was marginally related to HPV prevalence. Patients with severe immune depression were strongly predisposed to HPV infection (from 90.9% of patients carrying less than 200 cells/mm3 to 45.5% in those with CD4 cell level above 500 cells/mm3) and the development of cervical lesions. There was no relation between HIV immune status and HPV type 16, but HIV seropositive women with abnormal cytology were three times more likely to harbor this HPV type. The high HPV prevalence as opposed to absence of cancer cases was probably due to the moderate immune status of the women studied added to routine cancer prevention exams. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.15
{"title":"HUMAN PAPILLOMAVIRUS INFECTION IN HUMAN IMMUNODEFICIENCY VIRUS POSITIVE WOMEN UNDER ROUTINE PAP SMEAR","authors":"L. Oliveira, Jacqueline G. S. Haimuri, C. Jacyntho, Tomas Z Barrese, G. Vasconcelos, S. Cavalcanti, M. Rosa","doi":"10.17525/VRRJOURNAL.V13I1-2.15","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V13I1-2.15","url":null,"abstract":"The present study aimed to investigate HPV prevalence associated with cervical cytology and to evaluate data from 140 HIV positive women routinely attended at an out-patient public gynecological service in Rio de Janeiro state. The CD4 cell counts and HIV-1 RNA levels were determined using standardized protocols. HPV status and HPV 16 detection were ascertained respectively by My09/11 consensus primers and type specific primers. Colpocytology was performed at the first or subsequent visit to the service. Among women, 98.6% of them were under antiviral treatment, 92.1% had over 200 CD4 cells/mm3, 62.9% had detectable HIV RNA, 60.7% were HPV infected and 21.2 % harbored the HPV type 16. Normal/inflammatory results were prevalent (57.1%) and 7.9% of the patients had HSIL. Studying demographic data and HIV markers that could affect HPV infection, we found that time elapsed since positive diagnosis of less than four years was a significant factor. First sexual intercourse beginning earlier than 17 years, people younger than 30 years and white ethnicity were also linked to HPV infection. Alcohol use was marginally related to HPV prevalence. Patients with severe immune depression were strongly predisposed to HPV infection (from 90.9% of patients carrying less than 200 cells/mm3 to 45.5% in those with CD4 cell level above 500 cells/mm3) and the development of cervical lesions. There was no relation between HIV immune status and HPV type 16, but HIV seropositive women with abnormal cytology were three times more likely to harbor this HPV type. The high HPV prevalence as opposed to absence of cancer cases was probably due to the moderate immune status of the women studied added to routine cancer prevention exams. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.15","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2008-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The circulation of human metapneumovirus is related to seasonal patterns of respiratory syncytial virus. The aim of this study was to determine the frequency of hMPV infection diagnosed by indirect immunofluorescence in children attending emergency department and general wards of Hospital Infantil Albert Sabin in Fortaleza, Ceara, northeastern Brazil, during the respiratory syncytial virus season in 2006. The analysis included 389 samples. Antigens of hPMV were detected in 39 samples. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.17
{"title":"DETECTION OF HUMAN METAPNEUMOVIRUS IN NASOPHARYNGEAL ASPIRATES OF BRAZILIAN CHILDREN BY IMMUNOFLUORESCENCE ASSAY","authors":"J. F. Ribeiro, F. Moura","doi":"10.17525/VRR.V13I1-2.17","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.17","url":null,"abstract":"The circulation of human metapneumovirus is related to seasonal patterns of respiratory syncytial virus. The aim of this study was to determine the frequency of hMPV infection diagnosed by indirect immunofluorescence in children attending emergency department and general wards of Hospital Infantil Albert Sabin in Fortaleza, Ceara, northeastern Brazil, during the respiratory syncytial virus season in 2006. The analysis included 389 samples. Antigens of hPMV were detected in 39 samples. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.17","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"25 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2008-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gomes, Débora M. Cerqueira, D. Falcão, F. S. Menezes, M. Wigg, G. Mendes, F. Martins, J.F.M. Silva, R. Kuster, M. Romanos
{"title":"IN VITRO ANTI-HSV-2 ACTIVITY OF ISOQUERCETIN FROM HYPTIS FASCICULATA BENTH.","authors":"M. Gomes, Débora M. Cerqueira, D. Falcão, F. S. Menezes, M. Wigg, G. Mendes, F. Martins, J.F.M. Silva, R. Kuster, M. Romanos","doi":"10.17525/VRR.V13I1-2.4","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.4","url":null,"abstract":"","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-10-20DOI: 10.17525/VRRJOURNAL.V13I1-2.16
D. Vieira, Glauciane da Silva Bifano, E. Honda, M. Tada, W. C. Batista
Dengue Fever is a serious public health problem in tropical and subtropical regions of the world. The state of Rondonia has 52 municipalities, of which 32 are found inside Amazonia Legal and 20 in border areas. In the period of January 2004 until May 2006, 160 blood samples were colleted from patients suspected of having DENV disease during outbreaks of dengue fever in the municipalities of Ariquemes, Jaru, Ouro Preto do Oeste, Cacoal, Colorado do Oeste, Vilhena and Porto Velho. Samples were submitted for viral isolation following RNA viral extraction and RT-PCR, in order to identify the dengue virus. Afterwards, hemi-nested-PCR was used to identify the viral serotype. Seventy eight samples were characterized as DENV-3. The amplicons characterized by hemi-nested-PCR were sequenced for similar research with nucleotide sequences of DENV-3 deposited in Genbank, using the BLAST program. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.16
{"title":"ISOLATION AND IDENTIFICATION OF DENGUE VIRUS SEROTYPE 3 IN RONDONIA, BRAZIL","authors":"D. Vieira, Glauciane da Silva Bifano, E. Honda, M. Tada, W. C. Batista","doi":"10.17525/VRRJOURNAL.V13I1-2.16","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V13I1-2.16","url":null,"abstract":"Dengue Fever is a serious public health problem in tropical and subtropical regions of the world. The state of Rondonia has 52 municipalities, of which 32 are found inside Amazonia Legal and 20 in border areas. In the period of January 2004 until May 2006, 160 blood samples were colleted from patients suspected of having DENV disease during outbreaks of dengue fever in the municipalities of Ariquemes, Jaru, Ouro Preto do Oeste, Cacoal, Colorado do Oeste, Vilhena and Porto Velho. Samples were submitted for viral isolation following RNA viral extraction and RT-PCR, in order to identify the dengue virus. Afterwards, hemi-nested-PCR was used to identify the viral serotype. Seventy eight samples were characterized as DENV-3. The amplicons characterized by hemi-nested-PCR were sequenced for similar research with nucleotide sequences of DENV-3 deposited in Genbank, using the BLAST program. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.16","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Considerations about the development of the anti-rabies vaccine based on the analysis of historical documents and published scientific articles including correlations with the effect mediated by interferon – interferosis, are presented. In this review, an updated philosophical interpretation is presented on the technical report elaborated by Dr. Prof. August F. Dos Santos, professor at the College of Medicine and doctor of the Santa Casa de Misericordia of Rio de Janeiro, referring to the training undergone in France under Louis Pasteur’s orientation, during the period of 06/1886 - 07/1887, as well as other published documents of marked impact up to the mid-XXth century. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.18
通过对历史文献和已发表的科学文章的分析，包括与干扰素-干扰素介导的作用的相关性，提出了抗狂犬病疫苗发展的几点考虑。在这篇综述中，对August F. Dos Santos博士教授(医学院教授，巴西里约热内卢圣米塞里科迪医院医生)撰写的技术报告进行了更新的哲学解释，该技术报告涉及1886年6月至1887年7月期间在路易·巴斯德指导下在法国接受的培训，以及直到20世纪中叶具有显著影响的其他已发表的文件。DOI: http://dx.doi.org/10.17525/vrr.v13i1 - 2.18
{"title":"THE GENESIS OF RABIES PREVENTION INDUCED BY PASTEUR’S VACCINES AND THE PROTECTIVE PHENOMENON MEDIATED BY INTERFERONS","authors":"P. C. Romijn, M. C. Cabral","doi":"10.17525/VRR.V13I1-2.18","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.18","url":null,"abstract":"Considerations about the development of the anti-rabies vaccine based on the analysis of historical documents and published scientific articles including correlations with the effect mediated by interferon – interferosis, are presented. In this review, an updated philosophical interpretation is presented on the technical report elaborated by Dr. Prof. August F. Dos Santos, professor at the College of Medicine and doctor of the Santa Casa de Misericordia of Rio de Janeiro, referring to the training undergone in France under Louis Pasteur’s orientation, during the period of 06/1886 - 07/1887, as well as other published documents of marked impact up to the mid-XXth century. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.18","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2008-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Cavalcanti, L. Afonso, N. Moyses, I. M. Magalhães, M. Passos, L. Oliveira
Human papillomavirus (HPV) infections of the genital tract are the most prevalent sexually transmitted viruses worldwide. Oncogenic HPV types cause pre-malignant lesions that can progress to cervical carcinoma. In the male genital tract, most HPV infections are sub-clinical and associated with a vicious circle [stet] of treatment-reinfection of women. Nevertheless, HPV pathogenic processes are still poorly understood. The literature suggests that different HPV types can be found among sexual partners. In our study, we aimed to verify HPV infections in female patients as well as in their sexual partners, to test this hypothesis. The HPV DNA prevalence in women with Cervical Intraepithelial Neoplasia (CIN) was 92.5% compared with 25% for normal women, with a statistically significant difference (p<0.001). In male samples, the HPV DNA prevalence in partners from CIN women was 50% and for normal women partners, it was 17.5%. In the group of CIN women, we observed that 20 couples had HPV DNA in both partners. However, only 50% of the couples shared the same HPV type. In the group of normal women, only 6 couples were simultaneously infected by HPV, and from them only 33.3% had the same virus type. These results may be attributed to differences in local immunity and organization of the genital epithelia of each sex. On the other hand, female lesions would not be the result of re-infection by sexual partners, but rather a true recurrence of a latent infection. Finally, such 40% of agreement among all couples leads us to suggest a vicious circle [stet] of infectious processes, perpetuating HPV in the sexually active population. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.20
{"title":"HUMAN PAPILLOMAVIRUS (HPV) INFECTION OF SEXUAL PARTNERS OF WOMEN PRESENTING CERVICAL INTRAEPITHELIAL NEOPLASIA","authors":"S. Cavalcanti, L. Afonso, N. Moyses, I. M. Magalhães, M. Passos, L. Oliveira","doi":"10.17525/VRR.V13I1-2.20","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.20","url":null,"abstract":"Human papillomavirus (HPV) infections of the genital tract are the most prevalent sexually transmitted viruses worldwide. Oncogenic HPV types cause pre-malignant lesions that can progress to cervical carcinoma. In the male genital tract, most HPV infections are sub-clinical and associated with a vicious circle [stet] of treatment-reinfection of women. Nevertheless, HPV pathogenic processes are still poorly understood. The literature suggests that different HPV types can be found among sexual partners. In our study, we aimed to verify HPV infections in female patients as well as in their sexual partners, to test this hypothesis. The HPV DNA prevalence in women with Cervical Intraepithelial Neoplasia (CIN) was 92.5% compared with 25% for normal women, with a statistically significant difference (p<0.001). In male samples, the HPV DNA prevalence in partners from CIN women was 50% and for normal women partners, it was 17.5%. In the group of CIN women, we observed that 20 couples had HPV DNA in both partners. However, only 50% of the couples shared the same HPV type. In the group of normal women, only 6 couples were simultaneously infected by HPV, and from them only 33.3% had the same virus type. These results may be attributed to differences in local immunity and organization of the genital epithelia of each sex. On the other hand, female lesions would not be the result of re-infection by sexual partners, but rather a true recurrence of a latent infection. Finally, such 40% of agreement among all couples leads us to suggest a vicious circle [stet] of infectious processes, perpetuating HPV in the sexually active population. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.20","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2008-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. O. T. Carrasco, O. C. F. Neto, S. L. S. Lages, R. Sousa, A. B. Junior, A. A. Pinto
The breeding of ratites (ostriches, emus and rheas) has expanded considerably all over the world in recent years. The hemagglutination inhibition (HI) test is considered to be the standard serology test, for detection of antibodies against Newcastle Disease Virus (NDV). However, HI test tends to give false positive results in serum samples of some species, including ostriches. In this study, kaolin was used to eliminate the nonspecific hemagglutination inhibitors in ostrich serum samples. Twenty-seven non-kaolin-treated ostrich samples reacted to the test and the titres ranged from 2 to 64. However, these samples did not react when they were kaolin-treated. The controls did not show any variance on antibodies titres, regardless of treatment with kaolin. The use of kaolin in the routine serodiagnosis of ND not only relieves adsorption of serum samples with avian erythrocytes, but also contributes to the reduction in the maintenance of red blood cells donor birds. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.3
{"title":"ADAPTATION OF HEMAGGLUTINATION INHIBITION TECHNIQUE (HI) FOR THE DIAGNOSIS OF NEWCASTLE DISEASE IN OSTRICHES (Struthio camellus)","authors":"A. O. T. Carrasco, O. C. F. Neto, S. L. S. Lages, R. Sousa, A. B. Junior, A. A. Pinto","doi":"10.17525/VRR.V13I1-2.3","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.3","url":null,"abstract":"The breeding of ratites (ostriches, emus and rheas) has expanded considerably all over the world in recent years. The hemagglutination inhibition (HI) test is considered to be the standard serology test, for detection of antibodies against Newcastle Disease Virus (NDV). However, HI test tends to give false positive results in serum samples of some species, including ostriches. In this study, kaolin was used to eliminate the nonspecific hemagglutination inhibitors in ostrich serum samples. Twenty-seven non-kaolin-treated ostrich samples reacted to the test and the titres ranged from 2 to 64. However, these samples did not react when they were kaolin-treated. The controls did not show any variance on antibodies titres, regardless of treatment with kaolin. The use of kaolin in the routine serodiagnosis of ND not only relieves adsorption of serum samples with avian erythrocytes, but also contributes to the reduction in the maintenance of red blood cells donor birds. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.3","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2008-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dengue fever is considered the most important and widespread reemerging infectious disease in developing countries. Dengue virus belongs to the family Flaviviridae, a group of positive sense RNA viruses of 11 kb that are divided into four antigenically distinct serotypes (DENV-1 - DENV-4). Nowadays prevention is accomplished by using epidemiologic surveillance and vector control. Strain typing is a powerful tool for determining the geographic distribution of strains and understanding the epidemiology of infectious diseases. Strain typing can provide information on the relationship between disease manifestations and severity of disease. Harris et al. (Virology 253: 86-95, 1990), developed the RSS-PCR in order to genotype samples of Dengue virus, a rapid molecular strain-typing method that can be applied to analyze a large number of strains. The subtyping may identify viral factors that contribute to disease severity. This study was developed in order to genotype samples circulating in Minas Gerais state. Among the 34 clinical samples tested, 3 were DENV-1, 14 were DENV-2 and 17 were DENV-3. The samples were isolated using C6/36 cells. After RNA extraction using the silica method, RSS-PCR was executed. Different samples were used (1992-2005), including cases of DF (dengue fever) and DHF (dengue hemorrhagic fever). The results suggest genetic variability among the samples. The patterns seem to be related to the period of isolation, whether recent or from retrospective samples, as differences in patterns linked to severity of disease were not seen. The older sample (1992) seemed to have the same pattern as that ascribed by Harris et al, (1990) to the genotype “American”. The other samples showed patterns not related to any other previously described genotype. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.19
{"title":"GENOTYPING OF DENGUE VIRUS 2 USING RESTRICTION SITE-SPECIFIC (RSS)-PCR","authors":"C. Carrara, K. P. R. Souza, A. Cecílio","doi":"10.17525/VRR.V13I1-2.19","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.19","url":null,"abstract":"Dengue fever is considered the most important and widespread reemerging infectious disease in developing countries. Dengue virus belongs to the family Flaviviridae, a group of positive sense RNA viruses of 11 kb that are divided into four antigenically distinct serotypes (DENV-1 - DENV-4). Nowadays prevention is accomplished by using epidemiologic surveillance and vector control. Strain typing is a powerful tool for determining the geographic distribution of strains and understanding the epidemiology of infectious diseases. Strain typing can provide information on the relationship between disease manifestations and severity of disease. Harris et al. (Virology 253: 86-95, 1990), developed the RSS-PCR in order to genotype samples of Dengue virus, a rapid molecular strain-typing method that can be applied to analyze a large number of strains. The subtyping may identify viral factors that contribute to disease severity. This study was developed in order to genotype samples circulating in Minas Gerais state. Among the 34 clinical samples tested, 3 were DENV-1, 14 were DENV-2 and 17 were DENV-3. The samples were isolated using C6/36 cells. After RNA extraction using the silica method, RSS-PCR was executed. Different samples were used (1992-2005), including cases of DF (dengue fever) and DHF (dengue hemorrhagic fever). The results suggest genetic variability among the samples. The patterns seem to be related to the period of isolation, whether recent or from retrospective samples, as differences in patterns linked to severity of disease were not seen. The older sample (1992) seemed to have the same pattern as that ascribed by Harris et al, (1990) to the genotype “American”. The other samples showed patterns not related to any other previously described genotype. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.19","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2008-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, O. Barth, H. Schatzmayr
Orthopoxvirus infections in dairy cattle have been followed in the northwestern region of the state of Rio de Janeiro for the last nine years. Clinical specimens from 50 animals collected by Animal Health authorities have been received in our laboratory for laboratory diagnosis, most of them blood specimens. Orthopoxvirus infections have been confirmed in 46 animals by at least one of the laboratory tests employed: neutralizing antibody detection using as antigen a vaccinia-like strain molecularly characterized and isolated in the area; RT-PCR test; electron microscopy observation; and virus isolation in a Vero cell line. The virus strains obtained were identified as vaccinia-like by electron microscopy and reverse transcriptase polymerase chain reaction (RT-PCR), confirming the circulation of these strains in nature. Human cases associated with the infected animals have been also observed in the region, characterizing an expanding zoonosis caused by vaccinia-like strains in the state of Rio de Janeiro. DOI: http://dx.doi.org/10.17525/vrr.v12i1-2.10
{"title":"ANIMAL INFECTIONS BY VACCINIA-LIKE VIRUSES IN THE STATE OF RIO DE JANEIRO: 1- NORTHWESTERN REGION","authors":"Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, O. Barth, H. Schatzmayr","doi":"10.17525/VRR.V12I1-2.10","DOIUrl":"https://doi.org/10.17525/VRR.V12I1-2.10","url":null,"abstract":"Orthopoxvirus infections in dairy cattle have been followed in the northwestern region of the state of Rio de Janeiro for the last nine years. Clinical specimens from 50 animals collected by Animal Health authorities have been received in our laboratory for laboratory diagnosis, most of them blood specimens. Orthopoxvirus infections have been confirmed in 46 animals by at least one of the laboratory tests employed: neutralizing antibody detection using as antigen a vaccinia-like strain molecularly characterized and isolated in the area; RT-PCR test; electron microscopy observation; and virus isolation in a Vero cell line. The virus strains obtained were identified as vaccinia-like by electron microscopy and reverse transcriptase polymerase chain reaction (RT-PCR), confirming the circulation of these strains in nature. Human cases associated with the infected animals have been also observed in the region, characterizing an expanding zoonosis caused by vaccinia-like strains in the state of Rio de Janeiro. DOI: http://dx.doi.org/10.17525/vrr.v12i1-2.10","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2007-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67514230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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