Virus Reviews Research最新文献

{"title":"DENGUE CURRENT STATUS AND PERSPECTIVES","authors":"R. Cunha","doi":"10.17525/VRR.V19I1.109","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.109","url":null,"abstract":"","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"9 1","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resumos: II Simpósio Avançado de Virologia Hermann Schatzmayr","authors":"Sociedade Brasileira de Virologia","doi":"10.17525/vrr.v19i1.110","DOIUrl":"https://doi.org/10.17525/vrr.v19i1.110","url":null,"abstract":"Annals of Hermann Schatzmayr Advanced Symposium of Virology. May, 13-15, 2013, Instituto Oswaldo Cruz.","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE LEGACY OF HERMANN SCHATZMAYR","authors":"L. T. Figueiredo","doi":"10.17525/vrr.v19i1.104","DOIUrl":"https://doi.org/10.17525/vrr.v19i1.104","url":null,"abstract":"Dr. Hermann Schatzmayr, born in 1936, was graduated in Veterinary Medicine from the Universidade Federal Rural do Rio de Janeiro (UFRRJ) in 1957. From 1957 through 1958 he was a trainee in microbiology,and served in the sta of Prof. Paulo de Goes at the Department of Microbiology of the Universidade Federal do Rio de Janeiro (UFRJ). In 1961 Dr. Schatzmayr received training at the Institute of Hygiene, in Vienna, Austria. In Vienna, he worked on arboviruses, including tick-borne encephalitis virus. In 1961 he joined the staff at Fundacao Oswaldo Cruz (FIOCRUZ), where he and Dr. Joaquim Travassos da Rosa created a laboratory for the study of polio. In that laboratory he isolated poliovirus, studied outbreaks of poliomyelitis, and prepared an imported Sabin vaccine for distribution by the Brazilian Ministry of Health. In 1966 Dr. Schatzmayr received his Ph.D. from the Universities of Giessen and Freiburg, Germany, working on poliovirus, supported by a Humboldt Foundation scholarship. In 1967, he created a Virology Laboratory at the Escola Nacional de Saude Publica (National School of Public Health, ENSP) to study poliomyelitis, hepatitis and rubella. Working for the Ministry of Health he was responsible for the production of smallpox vaccine in embryonated eggs. From 1967 through 1977 while at FIOCRUZ he conducted a number of studies, with emphasis on enteroviruses and hepatitis B. In 1975, to obtain the title of Docent from the Universidade Federal Fluminense (UFF), he presented a thesis dealing with virus pollution of the Santos Bay area,which pioneered the eld of environmental virology in Brazil. In 1977 he created the Virology Laboratories of the Instituto Oswaldo Cruz, at FIOCRUZ. Starting in 1982, he participated on many International Committees of PAHO and WHO, attending meetings worldwide to help creating eradication programs for smallpox, polio and measles. One of his greatest engagements was on the successful eradication programs for smallpox in Brazil and worldwide. Dr. Schatzmayr spearheaded the creation of laboratories for the diagnosis of dengue in FIOCRUZ and throughout Brazil, participated in successful initiatives to control poliomyelitis in Brazil, and found time to provide technical advice on virus diagnosis and on the evaluation of viral vaccines to many virologists and public health officials. As a man of many accomplishments, he used to say that “as a researcher, I have worked on public health, trying to contribute to the solution of practical problems”. Dr. Schatzmayr was a founder, a first president of the Brazilian Society for Virology (SBV), and for many years was the Chief Editor of the SBV’s journal ‘Virus Reviews and Research’. He was an Editorial Board member of the journals “Memorias do Instituto Oswaldo Cruz” and “Vaccine (London)”. He was recipient of a long term Level 1 Research Productivity Scholarship from the Brazilian Research Council (CNPq). Dr. Schatzmayr published over 200 papers in peer reviewed journals and","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIBODIES ANTI-ORTHOPOXVIRUS IN HEALTHY WORKERS WHO HANDLE ANIMALS AND IN PATIENTS WITH CUTANEOUS LESIONS COMPATIBLE WITH POXVIRUS INFECTION IN THE STATE OF RIO DE JANEIRO, BRAZIL","authors":"O. Barth, H. Schatzmayr, M. C. Gonçalves, D. F. Barreto-Vieira, J. Fernandes, M. Lima, Renata Carvalho de Oliveira, E. Lemos","doi":"10.17525/vrr.v19i1.105","DOIUrl":"https://doi.org/10.17525/vrr.v19i1.105","url":null,"abstract":"Th e incidence of poxvirosis, a zoonosis caused by viruses of the family Poxviridae, has increased over the past two \u0000decades in Brazil. Th e important features of the Orthopoxvirus infection include rapid spread, occurrence restricted \u0000to lactating cows, lack of hygiene, person-to-person spread, and occurrence at properties where milking is conducted. \u0000We detected the presence of vaccinia virus antibodies, specifi cally for Cantagalo virus, in samples from two distinct \u0000populations from the state of Rio de Janeiro: (i) healthy professionals who live in the State of Rio de Janeiro and handle \u0000animals and (ii) in patients with cutaneous lesions compatible with poxvirosis. Th e plaque reduction neutralisation \u0000test (PRNT) was used. Of the 136 samples from the group of healthy professionals, 12 displayed seroreactivity with \u0000titres lower than or equal to 1/10. Anti-poxvirus antibodies were detected in 68.3% of samples of patients suspected to \u0000be infected with poxvirus (28/41). Th erefore, the plaque reduction neutralisation test constitutes a sensitive assay that \u0000can be more commonly used for diagnosis. Furthermore, professionals who handle animals should be included as an \u0000occupational risk group in areas with confi rmed human poxvirus cases.","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"19 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamers in the diagnosis of Rickettsiosis sensu lato","authors":"D. Godoy, S. Missailidis, A. Favacho, M. A. Mares-Guia, T. Rozental, E. Lemos","doi":"10.17525/VRR.V19I1.111","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.111","url":null,"abstract":"Aptamers are short synthetic oligonucleotides that specifi cally bind to various molecular targets with high affi nity and \u0000selectivity. Aptamers have found two main applications in bacteriology, diagnosis in various sensing applications and \u0000riboswitches. Rickettsiosis are diseases caused by rickettsias ‘sensu lato’ including spotted fever, typhus, anaplasmosis, \u0000ehrlichiosis, bartonellosis and Q fever. Traditional methodologies for the diagnosis of diseases associated with \u0000rickettsias ‘sensu lato’ are based on serological testing, bacterial cultures and molecular assays. However, an increasing \u0000number of novel technologies, including aptamer-based diagnostic sensors, are now on the horizon, opening up \u0000possibilities for earlier diagnosis and more sensitive assays. Th is perspective looks at the contribution of aptamers to \u0000rickettsias ‘sensu lato’ diagnosis, providing information on the ‘state of the art’ in this emerging field","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"31 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOSAFETY ACTIVITIES IN FIELD WITH WILD RODENTS","authors":"Renata Carvalho de Oliveira, E. Lemos","doi":"10.17525/VRR.V19I1.107","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.107","url":null,"abstract":"In the 1990s, with the identifi cation of hantavirus pulmonary syndrome (HPS) and the risk of occupational transmission, \u0000conservative biosafety measures in handling rodents potentially infected with hantavirus have been established for \u0000researchers in the fi eld. Recently, with the advanced knowledge about the biology and etiology of HPS and based on \u0000serological surveys for professionals who regularly handle rodents, it has been observed that the risk of contracting \u0000the disease is exceedingly low. Groups of professionals in the fi eld of zoology and ecology, among others, began to \u0000question the need for an adaptation of conservative protective measures previously published. In this context, taking \u0000into account some unintended negative and undesirable consequences during fi eldwork with the overprescription of \u0000preventative measures, in 2008, the American Society of Mammalogists provided revised guidelines for personnel \u0000working with rodents potentially infected with viruses that cause HPS in humans, considering that the level of HPS \u0000protection should be proportional to the level of risk exposure observed in many fi eld activities.","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"39 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RISK SCENARIO FOR HUMAN RABIES TRANSMITTED BY WILD ANIMALS IN BRAZIL","authors":"P. C. Romijn, L. Kimura, Nélio Moraes, B. N. Rolim, M. F. Teixeira, F. A. S. Junior, J. D. C. O. Filho, Maria Cristina Schneider","doi":"10.17525/vrr.v19i1.108","DOIUrl":"https://doi.org/10.17525/vrr.v19i1.108","url":null,"abstract":"Rabies is a public health and animal health issue with environmental, social and economic drivers and impacts. For decades, Lyssaviruses diagnostic research has been carried out independently at laboratories of several States of Brazil. In some areas activities have been developed for rabies prevention, such as the monitoring of the risk and also population control of haemathophagous bats. During the last years, however, the number of reports on human cases of rabies transmitted by bats and others wildlife animals in the North and North-East of Brazil increased, and new challenges had occur. Adequate dealing with situations of infected non-human primates (Callithrix jacchus) attacking humans and transmitting Lyssavirus in Ceara and D. rotundus spoliating pets, mainly dogs, in Rio de Janeiro has to be deployed. The feeding of Desmodus rotundus on pets has been reported lately with much more frequency close tourban areas, and eventually even persons are the victims. The new human case of marmoset-transmitted rabies in the State of Rio Grande do Norte beginning 2014, currently under investigation, demonstrate the urgency of information of technology and intersectoral investigations, allowing immediate follow-up, feed-bac and studies towards nidality, causes and possible solutions for these high risk events, starting at the local level. Usually rabies cases occurred invulnerable populations and to address this neglected disease, there is need of awareness and educational activities that induce the affected population to look for post exposure prophylaxis in case of risk and also strengthening the training for “One Health” professionals to identify risk areas and for monitoring, prevention and interventions. DOI: http://dx.doi.org/10.17525/vrr.v19i1.108","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"289 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2014-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISOENZYME ELECTROPHORESIS AND KARYOTYPE TECHNIQUES AS AN ALTERNATIVE FOR CELL LINE IDENTITY CONFIRMATION","authors":"J. Machado, A. Miranda, C. R. Gonçalves, T. I. Ikeda, Á. S. Cruz","doi":"10.17525/VRR.V19I1.90","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.90","url":null,"abstract":"The use of cell culture in biomedical science has been constantly enhanced and the use of certified and microrganisms-free cell lines is indispensable. Due to the value and the difficulty of acquiring a commercially available kit (AuthentikitTM system) for our routine in addition with the purpose of monitoring and ensuring quality of provided cell lines, and also contributing with cell characterization, the Nucleo de Cultura de Celulas do Instituto Adolfo Lutz (NCC– AL) padronized two techniques to identify the animal species from which the cells were originated: isoenzyme electrophoresis and karyotype. Cell extracts from 14 different cell lines were run under horizontal electrophoresis in agarose gel and lactate dehydrogenase isoenzyme profile was revealed using tetrazolium salt. The number of bands and the migration distances between bands of cell lines were similar to the literature, but small adjustments were needed to assist in the visualization of the bands that were not visible. Cell flasks for karyotype were incubated with colcemid solution and modal values of each cell line were calculated. Comparing all the results obtained with those reported in the literature, we conclude that both methods are promising and will allow better control of the services provided by the laboratory, not to mention guaranteeing authenticated cell lines for research and diagnostics. DOI:  http://dx.doi.org/10.17525/vrr.v19i1.90","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"19 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2014-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOLOGICAL, MOLECULAR AND IMMUNOCYTOCHEMICAL CHARACTERIZATION OF CELL CULTURE ADAPTED HUMAN ROTAVIRUS STRAINS DETECTED IN THE CITY OF PONTA GROSSA, PARANA, BRAZIL","authors":"A. C. Santos, F. Benati, F. Lauretti, R. Linhares, C. Nozawa","doi":"10.17525/VRR.V19I1.97","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.97","url":null,"abstract":"Rotaviruses (RV) are the single most important cause of severe diarrheal illness in infants and young children in both developed and developing countries worldwide. They affect mainly children less than 24 months of age. Presently, we adapted human rotavirus strains from field materials in cell culture, as well as, studied their properties by the analyses of the cytopathic effect, immunofluorescence features, plaque properties and virus genome stability. The study was carried out with 53 rotavirus-positive fecal specimens, collected in the city of Ponta Grossa, Parana, before the introduction of vaccination program in Brazil. The samples were submitted to at least 3 blind passages in MA-104 cell culture before discarded as non-cultivable. The adaptation process was monitored by the development of the cytopathic effect, immunofluorescence assay , plaque assay and genome stability by reverse transcriptase-polymerase chain reactions amplification. Fourteen human rotavirus strains were adapted to grow in cell culture. The isolation was demonstrated by the development of typical cytopathic effect. Immunofluorescence assay revealed granular inclusions with specific fluorescence mainly at the perinuclear area of the infected cells. Plaque assay allowed the demonstration of plaques with varying diameter depending upon the strains. Reverse transcriptase-polymerase chain reactions resulted in amplification and genotyping, demonstrating the stability of the genome through cell culture adaptation. DOI:  http://dx.doi.org/10.17525/vrr.v19i1.97","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"19 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2014-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RESPIRATORY SYNCYTIAL VIRUS: FROM DISCOVERY TO TREATMENT","authors":"D. C. Chong-Silva, N. Rosário","doi":"10.17525/VRR.V19I1.98","DOIUrl":"https://doi.org/10.17525/VRR.V19I1.98","url":null,"abstract":"To review the epidemiology, identification, clinical patterns and treatment of respiratory syncytial virus infection. Raised available studies in the MEDLINE database using the keywords respiratory syncytial virus, respiratory infection, bronchiolitis and indirect immunofluorescence in international studies since 1980. Respiratory Syncytial Virus (RSV) was first time isolated in 1956 and became the most frequently agent found, especially in the lower respiratory tract of children. It is responsible for significant morbidity and mortality in children younger than 18 months with risk factors  such  as  prematurity  and  heart  disease.  It  shows  well-defined  distribution  along  the  year.  Histopathology, immunohistochemistry and standardized molecular techniques to identify the virus have been described. The specific treatment of RSV infection is still limited. Antiviral agents as ribavirin showed limited effectiveness and restricted to use in a few patients with severe heart disease. Vaccines needs further studies before marketed. Humanized monoclonal antibody - palivizumab - is safe, well tolerated and the most cost-effective when used in children at risk for severe RSV infection. The high rate of children hospital admissions by RSV infection is a public health issue. Treatment is yet  supportive  and  non  specific  according  to  severity.  Epidemiologic  surveillance,  routine  virus  identification and acessibility to health centers are the key points to control infection by this virus.","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"19 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2014-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}