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Transaminase Enzyme and Liver Histopatological Structure of Mice Facing to Smoke Cigarettes After Administerred with Enhalus acoroides Peel Extract 给药后小鼠转氨酶及肝脏组织结构的变化
Pub Date : 2023-04-30 DOI: 10.15294/biosaintifika.v15i1.40725
Rivanty Saulahirwan, H. Sinay, R. L. Karuwal
The objective of this research were aimed to determine the level of transaminase enzymes and the histopathological structure of mice liver exposed to cigarette smoke after treatment of E. acoroides peel extract. Completely randomized design was conducted with 3 doses treatment groups (P1, P2, P3) and a control group (K+, K-) with 3 times repeated. The test was carried out during 14 days for cigarette smoke, and 14 days for seagrass peel extract tretment. The data measured were the levels of SGOT, SGPT and were analyzed using the Anova test. Liver histopathology (necrosis, parenchymatous, hydropic degeneration and normal) were described descriptively. The results showed that the administration of seagrass E. acoroides peel extract had a significant effect on decreasing levels of SGOT, SGPT (p value 0.05) when compared with K+. The P3 treatment had lower levels of SGOT (80.07±3.1 IU/L) and SGPT (88.3±2.1 IU/L). Liver histopathology consist of 34 necrosis cells less than the positive control group (K+) and normal cells were 47, higher than the positive control group (K+). It can be concluded that  E. acoroides peel extract had a significant effect on SGOT, SGPT levels in mice exposed to cigarette smoke. Peel extract of E. acoroides 75 mg/kgBW (P3) was an effective dose to reduce the levels of SGOT, SGPT and improve the histopathological structure of mice liver exposed to cigarette smoke.  This research is expected to be useful for the community, especially in the coastal areas regarding the benefits of using E. acoroides as a therapeutic agent against free radicals originating from cigarette smoke.
本研究旨在探讨豚草皮提取物对小鼠肝脏转氨酶水平及组织病理结构的影响。采用完全随机设计,分为3个剂量治疗组(P1、P2、P3)和一个对照组(K+、K-),重复3次。试验期为香烟组14 d,海草皮提取物组14 d。测量的数据为SGOT、SGPT水平,并使用方差分析检验进行分析。肝组织病理(坏死,实质,积水变性和正常)描述描述性。结果表明,与K+相比,海草皮提取物对SGOT、SGPT水平有显著降低作用(p值0.05);P3组SGOT(80.07±3.1 IU/L)、SGPT(88.3±2.1 IU/L)水平较低。肝组织病理学:坏死细胞34个,少于阳性对照组(K+),正常细胞47个,高于阳性对照组(K+)。综上所述,芦笋皮提取物对吸烟小鼠血清SGOT、SGPT水平有显著影响。黄芪皮提取物75 mg/kgBW (P3)是降低香烟暴露小鼠肝脏SGOT、SGPT水平和改善肝脏组织病理结构的有效剂量。预计本研究将对社区,特别是沿海地区的社区提供有益的帮助,使其能够作为一种治疗来自香烟烟雾的自由基的药物。
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引用次数: 0
In Vitro Mutagenesis of Dendrobium gabriella suryajaya Using Ethyl Methane Sulfonate (EMS) and Plantlet Regeneration 甲烷磺酸乙酯(EMS)体外诱变及植株再生的研究
Pub Date : 2023-04-30 DOI: 10.15294/biosaintifika.v15i1.40997
Khairana Millennia Martha, P. Dewanti, Firdha Narulita Alfian
Plant breeding through mutation techniques has the main purpose to explore genetic diversity in the presence of useful traits for developing new plants. Ethyl Methane Sulfonate (EMS) is a widely used chemical to induce mutations in plants focused on obtaining genetic variation. EMS can induce random points of mutations and some of which can create new stop codons in the desired gene. EMS has been successfully used to generate morphological diversity and encourage the improvement of desired traits. The 3-month-old Dendrobium gabriella suryajaya Orchid protocorm-like bodies (PLB) treatment with EMS concentrations of 0.025%, 0.05%, and 0.075% can change genetic diversity, especially in leaf morphology. A total of 105 orchids were soaked in EMS solution at concentrations for 12 and 24 hours respectively. The phenotypic variations observed in this population include changes in leaf color and the number of buds. This EMS mutant population will be used for further studies including screening for various traits such as through ISSR analysis to determine the level of diversity. This research shows that mutagenesis using EMS can produce the amount of variability in Dendrobium. The generation of variability for desired traits resulted in the identification of several mutants with important agronomic characteristics that can be used as germplasm for improvement.
通过突变技术进行植物育种的主要目的是在存在有用性状的情况下探索遗传多样性,以开发新植物。甲烷磺酸乙酯(EMS)是一种广泛用于植物诱变的化学物质,其主要目的是获得遗传变异。EMS可以诱导随机突变点,其中一些突变可以在所需基因中产生新的终止密码子。EMS已成功地用于产生形态多样性和促进所需性状的改进。EMS浓度分别为0.025%、0.05%和0.075%,可改变3月龄盖布石斛原球茎样体(PLB)的遗传多样性,尤其是叶片形态。105株兰花分别在EMS溶液中浸泡12和24小时。在这个群体中观察到的表型变异包括叶片颜色和芽数的变化。该EMS突变群体将用于进一步的研究,包括筛选各种性状,如通过ISSR分析来确定多样性水平。本研究表明,利用EMS诱变可以在石斛中产生一定数量的变异。期望性状变异的产生导致了几个具有重要农艺特征的突变体的鉴定,这些突变体可以用作改良的种质。
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引用次数: 1
Bioremediation of Cadmium (Cd) in Batik Wastewater Using Different Carrier Media Containing Rhizobacteria 不同根际细菌载体对蜡染废水中镉的生物修复研究
Pub Date : 2023-04-30 DOI: 10.15294/biosaintifika.v15i1.37683
S. Lestari, O. Oedjijono, S. Santoso, D. F. Kusharyati, Ramadi Habib Fathurrohim
Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.
蜡染废水对环境和健康非常危险,因为它含有重金属,如镉(Cd),来源于萘酚和靛蓝溶胶染料。在实验室中,根瘤菌具有很大的解毒Cd的潜力。因此,它们应该包装在载体介质中,以确保它们在野外的长期生存能力。载体介质为泥炭、木屑、豆腐渣。本研究旨在从11个分离菌株中确定3种根瘤菌对Cd的最大耐受潜力、冷冻干燥后保持根瘤菌活力的最佳载体培养基,以及含有潜在根瘤菌的载体培养基对蜡染废水中Cd的还原效果。此外,采用完全随机和分图设计的实验方法。Rb1、Rb3和Rb6对cd的耐受性最强。以豆腐渣和泥炭为最佳载体培养基,Rb3和Rb6分别以木屑和豆腐渣和泥炭为最佳载体。分离物Rb3C3对Cd的降解率最高,为85.1%,其他分离物的降解率均低于80%。用载体培养基分离根瘤菌使其易于应用于田间规模,因为它已经含有细菌生长的营养来源,并且包装具有较长的保质期。
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引用次数: 0
Biodegradation Test of Polluted River Caused by Domestic Wastewater Using Indigenous Bacteria in the Way Tomu Watershed, Ambon City 安汶市托木流域生活污水污染河道的原生细菌生物降解试验
Pub Date : 2023-04-30 DOI: 10.15294/biosaintifika.v15i1.40016
Lidya Imelda Nelvi Ratu, C. A. Seumahu, Amos Killay
Nowadays, the rivers are often used by residents around the river as the final disposal of domestic wastewater that causes the rivers to become polluted. Biodegradation is an alternative to wastewater treatment as an effort to solve water pollution which is often carried out using bacteria that have the potential to decomposers in the biodegradation process, especially indigenous bacteria. This study aims to identify and obtain isolates, combinations, and characteristics of the indigenous bacteria isolate that have the potential as a biodegradation agent for polluted river caused by domestic wastewater in the Way Tomu watershed, Ambon City. In this study, isolates of indigenous bacteria that were isolated and purified were tested for protease and amylase enzyme activity to select isolates to be used in the biodegradation process testing consisting of parameters pH, COD, and TSS as indicators of the biodegradation. Afterward, the isolates that have the best potential as biodegradation agents will be characterized and biochemical tests will be carried out to identify the general characteristics of these isolates. The results showed that the combination of Genus Aeromonas, Bacillus, and Pseudomonas as isolates of indigenous bacteria that has the best potential as an agent for the biodegradation of polluted river caused by domestic wastewater. The benefit of this research is as initial information and consideration for alternative treatment of polluted rivers caused by domestic wastewater using indigenous bacteria isolates as an effort to resolve river pollution, especially the rivers in Ambon City.
如今,河流经常被河流周围的居民用作生活废水的最终处理,导致河流被污染。生物降解是废水处理的一种替代方法,是解决水污染的一种努力,通常使用在生物降解过程中具有分解者潜力的细菌,特别是本地细菌来进行。本研究旨在鉴定和获得具有作为安汶市Way Tomu流域生活污水污染河流生物降解剂潜力的本地分离菌的分离物、组合和特性。本研究通过对分离纯化的本土细菌进行蛋白酶和淀粉酶活性测试,选择分离菌株进行生物降解过程测试,以pH、COD和TSS参数作为生物降解指标。之后,将对最有可能作为生物降解剂的分离株进行表征,并进行生化试验以确定这些分离株的一般特征。结果表明,气单胞菌属、芽孢杆菌属和假单胞菌属作为分离的本地细菌最有潜力作为生物降解生活污水污染河流的药剂。本研究的好处是提供了初步信息,并考虑了使用本地细菌分离物对生活污水造成的污染河流进行替代处理,以解决河流污染问题,特别是安汶市的河流。
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引用次数: 0
Plant Regeneration in Amorphophallus muelleri Blume. through Organogenic 魔芋的植株再生研究。通过器官性的
Pub Date : 2023-04-29 DOI: 10.15294/biosaintifika.v15i1.40501
P. H. Hardjo, Agnes Natalia Wijaya, Wina Dian Savitri, Fenny Irawati
Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.
槟榔(Amorphophallus muelleri Blume.)常被用作功能性食品原料,导致其糖含量高。传统的无性繁殖和生殖繁殖的局限性,影响了porpora产量的下降以满足市场需求。为解决这一问题,采用植物组织培养技术快速繁殖porang的鳞茎。因此,本研究的目的是确定植物生长调节剂(pgr)的组合和浓度,如6-苄基氨基嘌呤(BAP)、萘乙酸(NAA)和噻唑脲(TDZ)对愈伤组织质量的影响,以及对porang快速繁殖的愈伤组织和芽的生长,因为pgr是影响离体繁殖成功的因素之一。本研究的实验设计采用完全随机设计,采用单因素方差分析(Anova)进行统计分析。以细胞分裂素和生长素的不同组合和浓度作为控制变量,观察了愈伤组织、芽和根的几个生长参数,表明了porang的快速有机发生。诱导芽的最佳培养基为Murashige和Skoog (MS)培养基,浓度为5.0 mg。L-1 BAP和0.2 mg。L-1 NAA上的芽数和芽高分别达到15±1.8 cm和(7.2±1.86)cm。芽形成期结束后,确定生根期适宜的培养基为1.0 mg的Murashige和Skoog (MS)培养基。L-1 NAA使生根率达到100%,生根时间提前。pgr对魔芋微繁的最优组合及浓度。应了解从鳞茎外植体增加porang的产量,以满足市场需求。
{"title":"Plant Regeneration in Amorphophallus muelleri Blume. through Organogenic","authors":"P. H. Hardjo, Agnes Natalia Wijaya, Wina Dian Savitri, Fenny Irawati","doi":"10.15294/biosaintifika.v15i1.40501","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40501","url":null,"abstract":"Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74998861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Docking of the Cannabis sativa L. Bioactive Compound Against Inflammation Induced by Cigarette Smoke Exposure 大麻生物活性化合物抗香烟烟雾诱导炎症的分子对接
Pub Date : 2023-04-15 DOI: 10.15294/biosaintifika.v15i1.43845
Lisdiana Lisdiana, Dewi Mustikaningtyas
Cigarette smoke can modulate and increase chronic inflammation of the respiratory tract. The constituents of cigarette smoke-induced inflammation can activate several cell signaling pathways, including mitogen-activated protein kinases (MAPK), nuclear factor kappa-B (NF-κB), signal transducer and activator of transcription (STAT), and activatory protein-1 (AP-1). Cannabis plant (Cannabis sativa L.) contains many phytochemical compounds including cannabinoids, terpenes and phenolic compounds, which have potential in medicine, one of them is as an anti-inflammatory. This study aims to determine the interactions formed between bioactive compounds from cannabis plants in anti-inflammatory activity caused by cigarette smoke induction in the JAK/STAT and MAPK pathways through a study in silico. This research method was carried out in a descriptive exploratory manner using online databases such as PubChem, PASS online, SEA (Similarity Ensemble Approach) and Swiss Target Prediction, STRING. Docking simulation was carried out using PyRx 0.8. Data from software and web devices were analyzed descriptively and compared with control compounds. The docking results show that the compounds from Cannabis sativa L. can act as an anti-inflammatory in the context of cigarette smoke-induced inflammation. This is indicated by the similarity of amino acid residues resulting from the interaction of the aspirin drug (control) with the anti-inflammatory receptor protein in the compound cannabidihydrophenanthrene with PDGFRA and KDR receptors and compounds cannabicyclol with AKT1 and KDR receptors. This is indicated by the presence of value affinity is low indicating a stable and strong bond.
香烟烟雾可以调节和增加呼吸道的慢性炎症。香烟诱导炎症的成分可激活多种细胞信号通路,包括丝裂原活化蛋白激酶(MAPK)、核因子κ b (NF-κB)、信号转导和转录激活因子(STAT)、活化蛋白-1 (AP-1)。大麻植物(Cannabis sativa L.)含有多种植物化学物质,包括大麻素、萜烯和酚类化合物,具有潜在的药用价值,其中一种是抗炎作用。本研究旨在通过计算机研究,确定大麻植物中生物活性化合物在香烟烟雾诱导的JAK/STAT和MAPK通路的抗炎活性中形成的相互作用。本研究方法采用描述性探索性的方式,使用PubChem、PASS online、SEA (Similarity Ensemble Approach)和Swiss Target Prediction、STRING等在线数据库。对接仿真采用PyRx 0.8进行。从软件和网络设备的数据进行描述性分析,并与对照化合物进行比较。对接结果表明,大麻中的化合物在香烟引起的炎症中具有抗炎作用。这是由阿司匹林药物(对照)与PDGFRA和KDR受体化合物大麻二氢菲和AKT1和KDR受体化合物大麻环酚中抗炎受体蛋白相互作用产生的氨基酸残基的相似性所表明的。这是由存在的价值亲和度低表明一个稳定和强的纽带。
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引用次数: 0
Effectiveness of a Teaching Material: News-Based Teaching of Mount Ungaran 教材的有效性:以新闻为基础的《云葛兰山》教学
Pub Date : 2023-04-15 DOI: 10.15294/biosaintifika.v15i1.43702
Sri Nuryani, Margareta Rahayuningsih, Andin Irsadi, Fidia Fibriana
The news-based teaching is a teaching material that contains news integrated with the results of exploring the biodiversity on Mount Ungaran for high school students in Biology subject. Teaching materials were prepared based on the results of the needs analysis. Early observation of High School 12 Semarang students showed that Biology learning sources were still insufficient to achieve basic competence. This study aimed to develop valid news-based teaching material of Mount Ungaran Biodiversity using the research and development (RD) method for effective cognitive, psychomotor, and affective learning outcomes. The research method subjected the students of High School 12 Semarang to explore the biodiversity news of Mount Ungaran on the websites. Also, the students searched the information from Research Development (RD). The results showed that (1) news-based teaching material on Mount Ungaran biodiversity was valid and appropriate as a learning source (96%), (2) and it was effective to improve the students’ cognitive, psychomotor, and affective learning outcomes of biodiversity topic.
新闻型教学是一种将新闻内容与探索云葛兰山生物多样性的成果相结合的高中生物学科教材。根据需求分析的结果编写了教材。对中学12年级三宝垄学生的早期观察表明,生物学习资源仍然不足以达到基本的能力。本研究旨在利用研发(RD)的方法,开发有效的基于新闻的Ungaran山生物多样性教材,以获得有效的认知、精神运动和情感学习效果。研究方法是让三宝垄高中12年级的学生在网站上探索Ungaran山的生物多样性新闻。此外,学生们还搜索了研发部(RD)的信息。结果表明:(1)新闻教材作为生物多样性学习资源的有效性和适宜性(96%);(2)新闻教材能够有效提高学生生物多样性主题的认知、精神运动和情感学习成果。
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引用次数: 0
Development and Validation Model of Peatland Conservation through Interdisciplinary Science Learning 基于跨学科科学学习的泥炭地保护模式开发与验证
Pub Date : 2023-04-15 DOI: 10.15294/biosaintifika.v15i1.43846
Santiani Santiani, Sri Ngabekti, Sudarmin Sudarmin, Ani Rusilowati
The peatland conservation model is generally developed based on the community. A new education-based conservation model was developed through temporary activities through seminars, workshops or non-formal learning. The aim of this research is to develop and validation a peatland conservation model through interdisciplinary science learning that connects universities, schools and communities in peatland conservation activities. The development of a peatland conservation model went through third stages following interdisciplinary research patterns. Validation was determined by content and empirical validation. The peatland conservation model consisted of three interrelated dimensions in peatland conservation, namely universities, schools and communities. The model was equipped with implementation tools in the field in the form of science learning tools. The form of a peatland conservation model book, teaching practice materials, an environmental literacy questionnaire, and an interdisciplinary thinking performance rubric were developed in this study. The validated aspects included theory, assumptions, and conceptual model representations with the Aiken score was 0.88 as a valid category and the ICC value of 0.457 showed that the reliability was not good. Empirical validation showed that the dimensions of the university, school and community constructs had internal consistency reliability and validity. The relation between dimension that universities had an influence on schools and society, and schools have an influence on society. The peatland conservation model was equipped with practical teaching learning tools that directly connected universities and schools. However, this model was also suitable for non-educational natural sciences courses or pure natural sciences to form interdisciplinary thinking skills and environmental literacy.
泥炭地保护模式一般以群落为基础。通过研讨会、讲习班或非正式学习等临时活动,开发了一种新的以教育为基础的保护模式。本研究的目的是通过跨学科的科学学习来开发和验证泥炭地保护模式,该模式将泥炭地保护活动中的大学、学校和社区联系起来。泥炭地保护模式的发展经历了跨学科研究模式的第三阶段。采用内容验证法和经验验证法进行验证。泥炭地保护模式包括大学、学校和社区三个相互关联的泥炭地保护维度。该模型以科学学习工具的形式配备了实地实施工具。本研究以泥炭地保护示范书、教学实践教材、环境素养问卷和跨学科思维表现量表的形式进行研究。被验证的方面包括理论、假设和概念模型表征,Aiken得分为0.88为有效类别,ICC值为0.457表明信度不佳。实证验证表明,大学、学校和社区三个维度具有内部一致性、信度和效度。大学对学校和社会的影响维度,以及学校对社会的影响维度之间的关系。泥炭地保护模式配备了直接连接大学和学校的实用教学工具。但这种模式也适用于非教育性自然科学课程或纯自然科学课程,形成跨学科的思维能力和环境素养。
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引用次数: 0
Virtual Experiments of DNA Isolation and Hybridoma Techniques to Improve Students' Critical Thinking Ability 提高学生批判性思维能力的DNA分离和杂交瘤技术虚拟实验
Pub Date : 2023-04-15 DOI: 10.15294/biosaintifika.v15i1.43847
Erna Noor Savitri, Andin Vita Amalia, Prasetyo Listiaji, Rifa Atunnisa, Fidia Fibriana
Virtual learning during the Covid-19 pandemic always requires innovation from educators to present effective and interesting learning. The case method is an alternative learning method that can be used to increase awareness responses to problems contextually. This study aims to determine the effectiveness of implementing the use of virtual DNA isolation experiments and hybridoma techniques on students' critical thinking skills. The research was conducted on students majoring in Integrated Science, Semarang State University in the 2021-2022 academic year. Samples from the population are divided into experimental class and control class. The research instrument used to measure critical thinking skills uses a test. The results of the assessment of critical thinking skills were analyzed using quantitative analysis. The results showed that the experimental class obtained an average critical thinking ability of 87.86 and the control class obtained an average critical thinking ability of 73.86. The results of the posttest difference test analysis between the experimental class and the control class obtained a sig. (2-tailed) 0.000 less than the t table significance value of 0.05. Based on these results it can be concluded that the implementation of the use of virtual DNA isolation experiments and hinridoma techniques is effective in increasing students' critical thinking skills. The novelty of the results is virtual experiments of DNA Isolation and Hibridoma can be an alternative to virtual practice for schools with incomplete laboratory facilities.
在2019冠状病毒病大流行期间,虚拟学习始终需要教育工作者的创新,以提供有效和有趣的学习。案例法是另一种学习方法,可用于提高对情境问题的意识反应。本研究旨在确定使用虚拟DNA分离实验和杂交瘤技术对学生批判性思维技能的有效性。这项研究是在2021-2022学年对三宝垄州立大学综合科学专业的学生进行的。总体样本分为实验班和对照组。用于衡量批判性思维能力的研究工具使用测试。运用定量分析方法对批判性思维能力的评估结果进行分析。结果表明,实验班学生的平均批判性思维能力为87.86,对照组学生的平均批判性思维能力为73.86。实验班与对照班的后验差异检验分析结果为sig.(双尾)0.000,小于t表显著性值0.05。基于这些结果,可以得出结论,利用虚拟DNA分离实验和hinridoma技术的实施对提高学生的批判性思维能力是有效的。结果的新颖之处在于DNA分离和hibrioma的虚拟实验可以作为实验室设施不完善的学校虚拟实践的替代方案。
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引用次数: 0
Antifungal Potential of Cell-Free Supernatant Produced by Keratinolytic Fungi against Ganoderma boninense 角质溶解真菌产生的无细胞上清液对牛灵芝的抑菌作用
Pub Date : 2023-02-28 DOI: 10.15294/biosaintifika.v15i1.38325
J. Mamangkey, M. K. Huda, Randi Aritonang
Keratinolytic is a microorganism ability to degrade keratin substrates. Fungi are known to produce the enzyme keratinase. This research was carried out aiming to know the keratinolytic fungi potential in inhibiting Ganoderma boninense fungi. Five isolates of keratinolytic fungi coded A 31, A 18, A 12, K 18, and A 29 have antagonistic ability against G. boninense. A 12 and A 18 had the largest inhibitory diameter of 25.23 mm and 24 mm, respectively, after 7 days of incubation. The ability of A 12 and A 18 antagonisms was observed by testing the supernatant of keratinolytic fungi incubated in a feather meal broth medium. An 18 has the largest antagonism percentage of inhibition at 84.72% against G. boninense, followed by A12 (77.78%) and combination treatment of A 12 + A 18 (73.55%) after 7 days of incubation. Keratinolytic fungi can also grow on minimum salt chitin medium (MSCM) and produced a hydrolysis zone after 3 days of incubation. G. boninense hyphae abnormalities were observed by using a 100x magnification microscope. Moreover, hyphae lysis and dwarf, curved, rolled, and curly hyphae were also observed after giving treatment on A 12, A 18, and the combination treatment of both isolates. These results showed that both keratinolytic fungi isolates and the supernatant are powerful biocontrol agents against G. boninense. There is no previous research report on endophytic fungi inhibiting the growth of Ganoderma boninense. In the future, keratinolytic fungi can be applied in agriculture technology.
角蛋白降解是一种微生物降解角蛋白底物的能力。众所周知,真菌能产生角化酶。本研究旨在了解角朊酶真菌抑制灵芝真菌的潜力。5株编码为a31、a18、a12、k18和a29的溶角真菌对牛角杆菌具有拮抗作用。a12和a18在培养7 d后的抑菌直径最大,分别为25.23 mm和24 mm。在羽毛粉肉汤培养基中培养角化菌上清,观察其对a12和a18的拮抗能力。孵育7 d后,a18对牛肉牛的抑制率最高,为84.72%,其次为A12(77.78%)和A12 + a18联合处理(73.55%)。溶角真菌也可以在最低盐几丁质培养基(MSCM)上生长,培养3天后产生水解区。用100倍放大镜观察G. boninense菌丝异常。此外,在a12、a18和两种菌株联合处理后,还观察到菌丝裂解和矮化、弯曲、卷曲和卷曲菌丝。结果表明,溶角真菌分离物和上清液都是有效的生物防治剂。目前尚无内生真菌抑制灵芝生长的研究报道。在未来,溶角真菌可以应用于农业技术。
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Biosaintifika Journal of Biology Biology Education
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