Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.40725
Rivanty Saulahirwan, H. Sinay, R. L. Karuwal
The objective of this research were aimed to determine the level of transaminase enzymes and the histopathological structure of mice liver exposed to cigarette smoke after treatment of E. acoroides peel extract. Completely randomized design was conducted with 3 doses treatment groups (P1, P2, P3) and a control group (K+, K-) with 3 times repeated. The test was carried out during 14 days for cigarette smoke, and 14 days for seagrass peel extract tretment. The data measured were the levels of SGOT, SGPT and were analyzed using the Anova test. Liver histopathology (necrosis, parenchymatous, hydropic degeneration and normal) were described descriptively. The results showed that the administration of seagrass E. acoroides peel extract had a significant effect on decreasing levels of SGOT, SGPT (p value 0.05) when compared with K+. The P3 treatment had lower levels of SGOT (80.07±3.1 IU/L) and SGPT (88.3±2.1 IU/L). Liver histopathology consist of 34 necrosis cells less than the positive control group (K+) and normal cells were 47, higher than the positive control group (K+). It can be concluded that E. acoroides peel extract had a significant effect on SGOT, SGPT levels in mice exposed to cigarette smoke. Peel extract of E. acoroides 75 mg/kgBW (P3) was an effective dose to reduce the levels of SGOT, SGPT and improve the histopathological structure of mice liver exposed to cigarette smoke. This research is expected to be useful for the community, especially in the coastal areas regarding the benefits of using E. acoroides as a therapeutic agent against free radicals originating from cigarette smoke.
{"title":"Transaminase Enzyme and Liver Histopatological Structure of Mice Facing to Smoke Cigarettes After Administerred with Enhalus acoroides Peel Extract","authors":"Rivanty Saulahirwan, H. Sinay, R. L. Karuwal","doi":"10.15294/biosaintifika.v15i1.40725","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40725","url":null,"abstract":"The objective of this research were aimed to determine the level of transaminase enzymes and the histopathological structure of mice liver exposed to cigarette smoke after treatment of E. acoroides peel extract. Completely randomized design was conducted with 3 doses treatment groups (P1, P2, P3) and a control group (K+, K-) with 3 times repeated. The test was carried out during 14 days for cigarette smoke, and 14 days for seagrass peel extract tretment. The data measured were the levels of SGOT, SGPT and were analyzed using the Anova test. Liver histopathology (necrosis, parenchymatous, hydropic degeneration and normal) were described descriptively. The results showed that the administration of seagrass E. acoroides peel extract had a significant effect on decreasing levels of SGOT, SGPT (p value 0.05) when compared with K+. The P3 treatment had lower levels of SGOT (80.07±3.1 IU/L) and SGPT (88.3±2.1 IU/L). Liver histopathology consist of 34 necrosis cells less than the positive control group (K+) and normal cells were 47, higher than the positive control group (K+). It can be concluded that E. acoroides peel extract had a significant effect on SGOT, SGPT levels in mice exposed to cigarette smoke. Peel extract of E. acoroides 75 mg/kgBW (P3) was an effective dose to reduce the levels of SGOT, SGPT and improve the histopathological structure of mice liver exposed to cigarette smoke. This research is expected to be useful for the community, especially in the coastal areas regarding the benefits of using E. acoroides as a therapeutic agent against free radicals originating from cigarette smoke.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82126230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.40997
Khairana Millennia Martha, P. Dewanti, Firdha Narulita Alfian
Plant breeding through mutation techniques has the main purpose to explore genetic diversity in the presence of useful traits for developing new plants. Ethyl Methane Sulfonate (EMS) is a widely used chemical to induce mutations in plants focused on obtaining genetic variation. EMS can induce random points of mutations and some of which can create new stop codons in the desired gene. EMS has been successfully used to generate morphological diversity and encourage the improvement of desired traits. The 3-month-old Dendrobium gabriella suryajaya Orchid protocorm-like bodies (PLB) treatment with EMS concentrations of 0.025%, 0.05%, and 0.075% can change genetic diversity, especially in leaf morphology. A total of 105 orchids were soaked in EMS solution at concentrations for 12 and 24 hours respectively. The phenotypic variations observed in this population include changes in leaf color and the number of buds. This EMS mutant population will be used for further studies including screening for various traits such as through ISSR analysis to determine the level of diversity. This research shows that mutagenesis using EMS can produce the amount of variability in Dendrobium. The generation of variability for desired traits resulted in the identification of several mutants with important agronomic characteristics that can be used as germplasm for improvement.
{"title":"In Vitro Mutagenesis of Dendrobium gabriella suryajaya Using Ethyl Methane Sulfonate (EMS) and Plantlet Regeneration","authors":"Khairana Millennia Martha, P. Dewanti, Firdha Narulita Alfian","doi":"10.15294/biosaintifika.v15i1.40997","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40997","url":null,"abstract":"Plant breeding through mutation techniques has the main purpose to explore genetic diversity in the presence of useful traits for developing new plants. Ethyl Methane Sulfonate (EMS) is a widely used chemical to induce mutations in plants focused on obtaining genetic variation. EMS can induce random points of mutations and some of which can create new stop codons in the desired gene. EMS has been successfully used to generate morphological diversity and encourage the improvement of desired traits. The 3-month-old Dendrobium gabriella suryajaya Orchid protocorm-like bodies (PLB) treatment with EMS concentrations of 0.025%, 0.05%, and 0.075% can change genetic diversity, especially in leaf morphology. A total of 105 orchids were soaked in EMS solution at concentrations for 12 and 24 hours respectively. The phenotypic variations observed in this population include changes in leaf color and the number of buds. This EMS mutant population will be used for further studies including screening for various traits such as through ISSR analysis to determine the level of diversity. This research shows that mutagenesis using EMS can produce the amount of variability in Dendrobium. The generation of variability for desired traits resulted in the identification of several mutants with important agronomic characteristics that can be used as germplasm for improvement.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89803045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.37683
S. Lestari, O. Oedjijono, S. Santoso, D. F. Kusharyati, Ramadi Habib Fathurrohim
Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.
{"title":"Bioremediation of Cadmium (Cd) in Batik Wastewater Using Different Carrier Media Containing Rhizobacteria","authors":"S. Lestari, O. Oedjijono, S. Santoso, D. F. Kusharyati, Ramadi Habib Fathurrohim","doi":"10.15294/biosaintifika.v15i1.37683","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.37683","url":null,"abstract":"Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79078655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.40016
Lidya Imelda Nelvi Ratu, C. A. Seumahu, Amos Killay
Nowadays, the rivers are often used by residents around the river as the final disposal of domestic wastewater that causes the rivers to become polluted. Biodegradation is an alternative to wastewater treatment as an effort to solve water pollution which is often carried out using bacteria that have the potential to decomposers in the biodegradation process, especially indigenous bacteria. This study aims to identify and obtain isolates, combinations, and characteristics of the indigenous bacteria isolate that have the potential as a biodegradation agent for polluted river caused by domestic wastewater in the Way Tomu watershed, Ambon City. In this study, isolates of indigenous bacteria that were isolated and purified were tested for protease and amylase enzyme activity to select isolates to be used in the biodegradation process testing consisting of parameters pH, COD, and TSS as indicators of the biodegradation. Afterward, the isolates that have the best potential as biodegradation agents will be characterized and biochemical tests will be carried out to identify the general characteristics of these isolates. The results showed that the combination of Genus Aeromonas, Bacillus, and Pseudomonas as isolates of indigenous bacteria that has the best potential as an agent for the biodegradation of polluted river caused by domestic wastewater. The benefit of this research is as initial information and consideration for alternative treatment of polluted rivers caused by domestic wastewater using indigenous bacteria isolates as an effort to resolve river pollution, especially the rivers in Ambon City.
{"title":"Biodegradation Test of Polluted River Caused by Domestic Wastewater Using Indigenous Bacteria in the Way Tomu Watershed, Ambon City","authors":"Lidya Imelda Nelvi Ratu, C. A. Seumahu, Amos Killay","doi":"10.15294/biosaintifika.v15i1.40016","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40016","url":null,"abstract":"Nowadays, the rivers are often used by residents around the river as the final disposal of domestic wastewater that causes the rivers to become polluted. Biodegradation is an alternative to wastewater treatment as an effort to solve water pollution which is often carried out using bacteria that have the potential to decomposers in the biodegradation process, especially indigenous bacteria. This study aims to identify and obtain isolates, combinations, and characteristics of the indigenous bacteria isolate that have the potential as a biodegradation agent for polluted river caused by domestic wastewater in the Way Tomu watershed, Ambon City. In this study, isolates of indigenous bacteria that were isolated and purified were tested for protease and amylase enzyme activity to select isolates to be used in the biodegradation process testing consisting of parameters pH, COD, and TSS as indicators of the biodegradation. Afterward, the isolates that have the best potential as biodegradation agents will be characterized and biochemical tests will be carried out to identify the general characteristics of these isolates. The results showed that the combination of Genus Aeromonas, Bacillus, and Pseudomonas as isolates of indigenous bacteria that has the best potential as an agent for the biodegradation of polluted river caused by domestic wastewater. The benefit of this research is as initial information and consideration for alternative treatment of polluted rivers caused by domestic wastewater using indigenous bacteria isolates as an effort to resolve river pollution, especially the rivers in Ambon City.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75057052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.15294/biosaintifika.v15i1.40501
P. H. Hardjo, Agnes Natalia Wijaya, Wina Dian Savitri, Fenny Irawati
Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.
{"title":"Plant Regeneration in Amorphophallus muelleri Blume. through Organogenic","authors":"P. H. Hardjo, Agnes Natalia Wijaya, Wina Dian Savitri, Fenny Irawati","doi":"10.15294/biosaintifika.v15i1.40501","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40501","url":null,"abstract":"Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74998861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.15294/biosaintifika.v15i1.43845
Lisdiana Lisdiana, Dewi Mustikaningtyas
Cigarette smoke can modulate and increase chronic inflammation of the respiratory tract. The constituents of cigarette smoke-induced inflammation can activate several cell signaling pathways, including mitogen-activated protein kinases (MAPK), nuclear factor kappa-B (NF-κB), signal transducer and activator of transcription (STAT), and activatory protein-1 (AP-1). Cannabis plant (Cannabis sativa L.) contains many phytochemical compounds including cannabinoids, terpenes and phenolic compounds, which have potential in medicine, one of them is as an anti-inflammatory. This study aims to determine the interactions formed between bioactive compounds from cannabis plants in anti-inflammatory activity caused by cigarette smoke induction in the JAK/STAT and MAPK pathways through a study in silico. This research method was carried out in a descriptive exploratory manner using online databases such as PubChem, PASS online, SEA (Similarity Ensemble Approach) and Swiss Target Prediction, STRING. Docking simulation was carried out using PyRx 0.8. Data from software and web devices were analyzed descriptively and compared with control compounds. The docking results show that the compounds from Cannabis sativa L. can act as an anti-inflammatory in the context of cigarette smoke-induced inflammation. This is indicated by the similarity of amino acid residues resulting from the interaction of the aspirin drug (control) with the anti-inflammatory receptor protein in the compound cannabidihydrophenanthrene with PDGFRA and KDR receptors and compounds cannabicyclol with AKT1 and KDR receptors. This is indicated by the presence of value affinity is low indicating a stable and strong bond.
{"title":"Molecular Docking of the Cannabis sativa L. Bioactive Compound Against Inflammation Induced by Cigarette Smoke Exposure","authors":"Lisdiana Lisdiana, Dewi Mustikaningtyas","doi":"10.15294/biosaintifika.v15i1.43845","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.43845","url":null,"abstract":"Cigarette smoke can modulate and increase chronic inflammation of the respiratory tract. The constituents of cigarette smoke-induced inflammation can activate several cell signaling pathways, including mitogen-activated protein kinases (MAPK), nuclear factor kappa-B (NF-κB), signal transducer and activator of transcription (STAT), and activatory protein-1 (AP-1). Cannabis plant (Cannabis sativa L.) contains many phytochemical compounds including cannabinoids, terpenes and phenolic compounds, which have potential in medicine, one of them is as an anti-inflammatory. This study aims to determine the interactions formed between bioactive compounds from cannabis plants in anti-inflammatory activity caused by cigarette smoke induction in the JAK/STAT and MAPK pathways through a study in silico. This research method was carried out in a descriptive exploratory manner using online databases such as PubChem, PASS online, SEA (Similarity Ensemble Approach) and Swiss Target Prediction, STRING. Docking simulation was carried out using PyRx 0.8. Data from software and web devices were analyzed descriptively and compared with control compounds. The docking results show that the compounds from Cannabis sativa L. can act as an anti-inflammatory in the context of cigarette smoke-induced inflammation. This is indicated by the similarity of amino acid residues resulting from the interaction of the aspirin drug (control) with the anti-inflammatory receptor protein in the compound cannabidihydrophenanthrene with PDGFRA and KDR receptors and compounds cannabicyclol with AKT1 and KDR receptors. This is indicated by the presence of value affinity is low indicating a stable and strong bond.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134999062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.15294/biosaintifika.v15i1.43702
Sri Nuryani, Margareta Rahayuningsih, Andin Irsadi, Fidia Fibriana
The news-based teaching is a teaching material that contains news integrated with the results of exploring the biodiversity on Mount Ungaran for high school students in Biology subject. Teaching materials were prepared based on the results of the needs analysis. Early observation of High School 12 Semarang students showed that Biology learning sources were still insufficient to achieve basic competence. This study aimed to develop valid news-based teaching material of Mount Ungaran Biodiversity using the research and development (RD) method for effective cognitive, psychomotor, and affective learning outcomes. The research method subjected the students of High School 12 Semarang to explore the biodiversity news of Mount Ungaran on the websites. Also, the students searched the information from Research Development (RD). The results showed that (1) news-based teaching material on Mount Ungaran biodiversity was valid and appropriate as a learning source (96%), (2) and it was effective to improve the students’ cognitive, psychomotor, and affective learning outcomes of biodiversity topic.
{"title":"Effectiveness of a Teaching Material: News-Based Teaching of Mount Ungaran","authors":"Sri Nuryani, Margareta Rahayuningsih, Andin Irsadi, Fidia Fibriana","doi":"10.15294/biosaintifika.v15i1.43702","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.43702","url":null,"abstract":"The news-based teaching is a teaching material that contains news integrated with the results of exploring the biodiversity on Mount Ungaran for high school students in Biology subject. Teaching materials were prepared based on the results of the needs analysis. Early observation of High School 12 Semarang students showed that Biology learning sources were still insufficient to achieve basic competence. This study aimed to develop valid news-based teaching material of Mount Ungaran Biodiversity using the research and development (RD) method for effective cognitive, psychomotor, and affective learning outcomes. The research method subjected the students of High School 12 Semarang to explore the biodiversity news of Mount Ungaran on the websites. Also, the students searched the information from Research Development (RD). The results showed that (1) news-based teaching material on Mount Ungaran biodiversity was valid and appropriate as a learning source (96%), (2) and it was effective to improve the students’ cognitive, psychomotor, and affective learning outcomes of biodiversity topic.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134999063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.15294/biosaintifika.v15i1.43846
Santiani Santiani, Sri Ngabekti, Sudarmin Sudarmin, Ani Rusilowati
The peatland conservation model is generally developed based on the community. A new education-based conservation model was developed through temporary activities through seminars, workshops or non-formal learning. The aim of this research is to develop and validation a peatland conservation model through interdisciplinary science learning that connects universities, schools and communities in peatland conservation activities. The development of a peatland conservation model went through third stages following interdisciplinary research patterns. Validation was determined by content and empirical validation. The peatland conservation model consisted of three interrelated dimensions in peatland conservation, namely universities, schools and communities. The model was equipped with implementation tools in the field in the form of science learning tools. The form of a peatland conservation model book, teaching practice materials, an environmental literacy questionnaire, and an interdisciplinary thinking performance rubric were developed in this study. The validated aspects included theory, assumptions, and conceptual model representations with the Aiken score was 0.88 as a valid category and the ICC value of 0.457 showed that the reliability was not good. Empirical validation showed that the dimensions of the university, school and community constructs had internal consistency reliability and validity. The relation between dimension that universities had an influence on schools and society, and schools have an influence on society. The peatland conservation model was equipped with practical teaching learning tools that directly connected universities and schools. However, this model was also suitable for non-educational natural sciences courses or pure natural sciences to form interdisciplinary thinking skills and environmental literacy.
{"title":"Development and Validation Model of Peatland Conservation through Interdisciplinary Science Learning","authors":"Santiani Santiani, Sri Ngabekti, Sudarmin Sudarmin, Ani Rusilowati","doi":"10.15294/biosaintifika.v15i1.43846","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.43846","url":null,"abstract":"The peatland conservation model is generally developed based on the community. A new education-based conservation model was developed through temporary activities through seminars, workshops or non-formal learning. The aim of this research is to develop and validation a peatland conservation model through interdisciplinary science learning that connects universities, schools and communities in peatland conservation activities. The development of a peatland conservation model went through third stages following interdisciplinary research patterns. Validation was determined by content and empirical validation. The peatland conservation model consisted of three interrelated dimensions in peatland conservation, namely universities, schools and communities. The model was equipped with implementation tools in the field in the form of science learning tools. The form of a peatland conservation model book, teaching practice materials, an environmental literacy questionnaire, and an interdisciplinary thinking performance rubric were developed in this study. The validated aspects included theory, assumptions, and conceptual model representations with the Aiken score was 0.88 as a valid category and the ICC value of 0.457 showed that the reliability was not good. Empirical validation showed that the dimensions of the university, school and community constructs had internal consistency reliability and validity. The relation between dimension that universities had an influence on schools and society, and schools have an influence on society. The peatland conservation model was equipped with practical teaching learning tools that directly connected universities and schools. However, this model was also suitable for non-educational natural sciences courses or pure natural sciences to form interdisciplinary thinking skills and environmental literacy.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134999061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.15294/biosaintifika.v15i1.43847
Erna Noor Savitri, Andin Vita Amalia, Prasetyo Listiaji, Rifa Atunnisa, Fidia Fibriana
Virtual learning during the Covid-19 pandemic always requires innovation from educators to present effective and interesting learning. The case method is an alternative learning method that can be used to increase awareness responses to problems contextually. This study aims to determine the effectiveness of implementing the use of virtual DNA isolation experiments and hybridoma techniques on students' critical thinking skills. The research was conducted on students majoring in Integrated Science, Semarang State University in the 2021-2022 academic year. Samples from the population are divided into experimental class and control class. The research instrument used to measure critical thinking skills uses a test. The results of the assessment of critical thinking skills were analyzed using quantitative analysis. The results showed that the experimental class obtained an average critical thinking ability of 87.86 and the control class obtained an average critical thinking ability of 73.86. The results of the posttest difference test analysis between the experimental class and the control class obtained a sig. (2-tailed) 0.000 less than the t table significance value of 0.05. Based on these results it can be concluded that the implementation of the use of virtual DNA isolation experiments and hinridoma techniques is effective in increasing students' critical thinking skills. The novelty of the results is virtual experiments of DNA Isolation and Hibridoma can be an alternative to virtual practice for schools with incomplete laboratory facilities.
{"title":"Virtual Experiments of DNA Isolation and Hybridoma Techniques to Improve Students' Critical Thinking Ability","authors":"Erna Noor Savitri, Andin Vita Amalia, Prasetyo Listiaji, Rifa Atunnisa, Fidia Fibriana","doi":"10.15294/biosaintifika.v15i1.43847","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.43847","url":null,"abstract":"Virtual learning during the Covid-19 pandemic always requires innovation from educators to present effective and interesting learning. The case method is an alternative learning method that can be used to increase awareness responses to problems contextually. This study aims to determine the effectiveness of implementing the use of virtual DNA isolation experiments and hybridoma techniques on students' critical thinking skills. The research was conducted on students majoring in Integrated Science, Semarang State University in the 2021-2022 academic year. Samples from the population are divided into experimental class and control class. The research instrument used to measure critical thinking skills uses a test. The results of the assessment of critical thinking skills were analyzed using quantitative analysis. The results showed that the experimental class obtained an average critical thinking ability of 87.86 and the control class obtained an average critical thinking ability of 73.86. The results of the posttest difference test analysis between the experimental class and the control class obtained a sig. (2-tailed) 0.000 less than the t table significance value of 0.05. Based on these results it can be concluded that the implementation of the use of virtual DNA isolation experiments and hinridoma techniques is effective in increasing students' critical thinking skills. The novelty of the results is virtual experiments of DNA Isolation and Hibridoma can be an alternative to virtual practice for schools with incomplete laboratory facilities.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"39 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134999060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-28DOI: 10.15294/biosaintifika.v15i1.38325
J. Mamangkey, M. K. Huda, Randi Aritonang
Keratinolytic is a microorganism ability to degrade keratin substrates. Fungi are known to produce the enzyme keratinase. This research was carried out aiming to know the keratinolytic fungi potential in inhibiting Ganoderma boninense fungi. Five isolates of keratinolytic fungi coded A 31, A 18, A 12, K 18, and A 29 have antagonistic ability against G. boninense. A 12 and A 18 had the largest inhibitory diameter of 25.23 mm and 24 mm, respectively, after 7 days of incubation. The ability of A 12 and A 18 antagonisms was observed by testing the supernatant of keratinolytic fungi incubated in a feather meal broth medium. An 18 has the largest antagonism percentage of inhibition at 84.72% against G. boninense, followed by A12 (77.78%) and combination treatment of A 12 + A 18 (73.55%) after 7 days of incubation. Keratinolytic fungi can also grow on minimum salt chitin medium (MSCM) and produced a hydrolysis zone after 3 days of incubation. G. boninense hyphae abnormalities were observed by using a 100x magnification microscope. Moreover, hyphae lysis and dwarf, curved, rolled, and curly hyphae were also observed after giving treatment on A 12, A 18, and the combination treatment of both isolates. These results showed that both keratinolytic fungi isolates and the supernatant are powerful biocontrol agents against G. boninense. There is no previous research report on endophytic fungi inhibiting the growth of Ganoderma boninense. In the future, keratinolytic fungi can be applied in agriculture technology.
{"title":"Antifungal Potential of Cell-Free Supernatant Produced by Keratinolytic Fungi against Ganoderma boninense","authors":"J. Mamangkey, M. K. Huda, Randi Aritonang","doi":"10.15294/biosaintifika.v15i1.38325","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.38325","url":null,"abstract":"Keratinolytic is a microorganism ability to degrade keratin substrates. Fungi are known to produce the enzyme keratinase. This research was carried out aiming to know the keratinolytic fungi potential in inhibiting Ganoderma boninense fungi. Five isolates of keratinolytic fungi coded A 31, A 18, A 12, K 18, and A 29 have antagonistic ability against G. boninense. A 12 and A 18 had the largest inhibitory diameter of 25.23 mm and 24 mm, respectively, after 7 days of incubation. The ability of A 12 and A 18 antagonisms was observed by testing the supernatant of keratinolytic fungi incubated in a feather meal broth medium. An 18 has the largest antagonism percentage of inhibition at 84.72% against G. boninense, followed by A12 (77.78%) and combination treatment of A 12 + A 18 (73.55%) after 7 days of incubation. Keratinolytic fungi can also grow on minimum salt chitin medium (MSCM) and produced a hydrolysis zone after 3 days of incubation. G. boninense hyphae abnormalities were observed by using a 100x magnification microscope. Moreover, hyphae lysis and dwarf, curved, rolled, and curly hyphae were also observed after giving treatment on A 12, A 18, and the combination treatment of both isolates. These results showed that both keratinolytic fungi isolates and the supernatant are powerful biocontrol agents against G. boninense. There is no previous research report on endophytic fungi inhibiting the growth of Ganoderma boninense. In the future, keratinolytic fungi can be applied in agriculture technology.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"340 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79744496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}