Pub Date : 2023-08-15DOI: 10.15294/biosaintifika.v15i2.46501
Noor Aini Habibah, Ananda Lutfiah, Alin Liana, Woro Anindito Sri Tunjung, Meti Indrowati, Furzani Pa’ee
Dayak onion contains bioactive compounds that are traditionally used for medicine. The production of bioactive compounds can be increased through callus culture. This study aims to analyze the effect of the combination of plant growth regulator on the growth of Dayak bulb callus. The design of this study used a completely randomized factorial design with 2 factors. The first factor is the concentration of auxin. While the second factor is the concentration of kinetin. The parameters observed include the percentage of callus, the time of callus formation, fresh weight, dry weight, and callus morphology. In this study, a callus growth curve was also made to determine the best harvest time. The results showed that highest callus fresh weight was obtained in the P3K1 treatment. The percentage of callus formation in the picloram + kinetin treatment was higher than in the 2,4-D + kinetin treatment. The time of callus formation occurred in the combination of picloram + kinetin faster than the 2.4-D + kinetin treatment. The callus color is diverse, while the texture of the entire callus is compact. The best callus induction medium for Dayak onion bulbs is to use picloram 2-4 ppm + kinetin0.025-0.5 ppm. The results of this study provide optimal growth regulatory information for the induction of dayak onion callus that has never been reported before. This information can be the basis for the development of methods of production of bioactive compounds from dayak onions through callus culture.
达亚克洋葱含有传统上用于医药的生物活性化合物。愈伤组织培养可以增加生物活性化合物的产生。本研究旨在分析植物生长调节剂组合对大芋球茎愈伤组织生长的影响。本研究采用2因素的完全随机因子设计。第一个因素是生长素的浓度。第二个因素是动蛋白的浓度。观察的参数包括愈伤组织百分率、愈伤组织形成时间、鲜重、干重和愈伤组织形态。本研究还绘制了愈伤组织生长曲线，以确定最佳采收期。结果表明，P3K1处理的愈伤组织鲜重最高。皮氯仑+激动素处理的愈伤组织形成率高于2,4- d +激动素处理。皮氯仑+激动素联合处理的愈伤组织形成时间比2.4-D +激动素处理的愈伤组织形成时间快。愈伤组织颜色多样，整个愈伤组织质地致密。大葱愈伤组织诱导的最佳培养基为picloram 2-4 ppm + kinetin0.025-0.5 ppm。本研究结果为大葱愈伤组织的诱导提供了前所未有的生长调控信息。这一信息可作为开发利用大葱愈伤组织培养生产生物活性化合物方法的基础。
{"title":"Callogenesis of Dayak Onion (Eleutherine palmifolia) Bulb in response of Picloram, 2,4-D, and Kinetin","authors":"Noor Aini Habibah, Ananda Lutfiah, Alin Liana, Woro Anindito Sri Tunjung, Meti Indrowati, Furzani Pa’ee","doi":"10.15294/biosaintifika.v15i2.46501","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i2.46501","url":null,"abstract":"Dayak onion contains bioactive compounds that are traditionally used for medicine. The production of bioactive compounds can be increased through callus culture. This study aims to analyze the effect of the combination of plant growth regulator on the growth of Dayak bulb callus. The design of this study used a completely randomized factorial design with 2 factors. The first factor is the concentration of auxin. While the second factor is the concentration of kinetin. The parameters observed include the percentage of callus, the time of callus formation, fresh weight, dry weight, and callus morphology. In this study, a callus growth curve was also made to determine the best harvest time. The results showed that highest callus fresh weight was obtained in the P3K1 treatment. The percentage of callus formation in the picloram + kinetin treatment was higher than in the 2,4-D + kinetin treatment. The time of callus formation occurred in the combination of picloram + kinetin faster than the 2.4-D + kinetin treatment. The callus color is diverse, while the texture of the entire callus is compact. The best callus induction medium for Dayak onion bulbs is to use picloram 2-4 ppm + kinetin0.025-0.5 ppm. The results of this study provide optimal growth regulatory information for the induction of dayak onion callus that has never been reported before. This information can be the basis for the development of methods of production of bioactive compounds from dayak onions through callus culture.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"206 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135163455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.15294/biosaintifika.v15i2.43236
Niken Subekti, Anita Fadhila
House construction continues from time to time, the frequency of termite attacks on buildings in the area is also very high due to the disturbance of the termite's natural habitat. Termites target the structural wood of buildings and objects derived from cellulose. This study aims to analyze the diversity of termite, house component damage, the relationship between the age and condition of building in Jakarta, Indonesia . This study used a purposive sampling method from 134 houses in Jakarta, Indonesia with a cross-sectional approach. The research procedures included taking and identifying specimens, analyze the intensity percentage of building damage, analyze the relationship between the age of the building and the condition of the building, and environmental factors. Based on the results of research conducted on 134 houses in Jakarta, it is concluded that there are four types of termites in the study sites: C. curvignathus (61.14%), M. gilvus (18.65%), M. inspiratus (10.88%), and C. cynocephaus (9.33%). The worst damage to residential components is found in the sills. Based on statistical tests, there is a correlation between age and the condition of the building. It indicates that the age of the building is significantly related to its condition.
{"title":"Termite Identification Attacks on Buildings","authors":"Niken Subekti, Anita Fadhila","doi":"10.15294/biosaintifika.v15i2.43236","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i2.43236","url":null,"abstract":"House construction continues from time to time, the frequency of termite attacks on buildings in the area is also very high due to the disturbance of the termite's natural habitat. Termites target the structural wood of buildings and objects derived from cellulose. This study aims to analyze the diversity of termite, house component damage, the relationship between the age and condition of building in Jakarta, Indonesia . This study used a purposive sampling method from 134 houses in Jakarta, Indonesia with a cross-sectional approach. The research procedures included taking and identifying specimens, analyze the intensity percentage of building damage, analyze the relationship between the age of the building and the condition of the building, and environmental factors. Based on the results of research conducted on 134 houses in Jakarta, it is concluded that there are four types of termites in the study sites: C. curvignathus (61.14%), M. gilvus (18.65%), M. inspiratus (10.88%), and C. cynocephaus (9.33%). The worst damage to residential components is found in the sills. Based on statistical tests, there is a correlation between age and the condition of the building. It indicates that the age of the building is significantly related to its condition.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135163453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.15294/biosaintifika.v15i2.44632
Agave Chintya Gabriella Sijabat, Sri Isdadiyanto, Agung Janika Sitasiwi
Intake of a high-fat diet can cause hyperlipidemia which increases the rate of triglycerides and cholesterol, and decrease the HDL levels. The increase of lipid levels in the blood can cause Non-Alcoholic Fatty Liver Disease (NAFLD) which triggers damage of liver function. Mahogany seeds have bioactive compounds that have the potential to be antioxidants in treating hyperlipidemia. This study aimed to analyze the liver function of rats by inducing a high-fat diet after giving mahogany seeds ethanol extract which was observed through the levels of SGOT and SGPT. Thirty male rats of the Sprague Dawley strain were divided into 6 groups of treatment, that were: P0 (only given commercial feed), P1 (given a high-fat diet), P2 (given a high-fat diet and simvastatin at a dose of 8 mg/200 g BW), P3, P4, and P5 (given a high-fat diet and mahogany seeds ethanol extract of 14, 28, and 56 mg/200gBW). The results showed that there was no significant difference between all treatment groups. Administration of mahogany seeds ethanol extract was able to prevent the increase of SGOT and SGPT levels. Mahogany seeds ethanol extract has the hepatoprotective effect against high-fat diet induced hepatotoxicity, thereby affirming its traditional therapeutic role in liver injury.
{"title":"Rat Liver Function Induced By A High-Fat Diet After Giving Mahogany Seeds Ethanol Extract","authors":"Agave Chintya Gabriella Sijabat, Sri Isdadiyanto, Agung Janika Sitasiwi","doi":"10.15294/biosaintifika.v15i2.44632","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i2.44632","url":null,"abstract":"Intake of a high-fat diet can cause hyperlipidemia which increases the rate of triglycerides and cholesterol, and decrease the HDL levels. The increase of lipid levels in the blood can cause Non-Alcoholic Fatty Liver Disease (NAFLD) which triggers damage of liver function. Mahogany seeds have bioactive compounds that have the potential to be antioxidants in treating hyperlipidemia. This study aimed to analyze the liver function of rats by inducing a high-fat diet after giving mahogany seeds ethanol extract which was observed through the levels of SGOT and SGPT. Thirty male rats of the Sprague Dawley strain were divided into 6 groups of treatment, that were: P0 (only given commercial feed), P1 (given a high-fat diet), P2 (given a high-fat diet and simvastatin at a dose of 8 mg/200 g BW), P3, P4, and P5 (given a high-fat diet and mahogany seeds ethanol extract of 14, 28, and 56 mg/200gBW). The results showed that there was no significant difference between all treatment groups. Administration of mahogany seeds ethanol extract was able to prevent the increase of SGOT and SGPT levels. Mahogany seeds ethanol extract has the hepatoprotective effect against high-fat diet induced hepatotoxicity, thereby affirming its traditional therapeutic role in liver injury.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135163450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.15294/biosaintifika.v15i2.38303
Desy Irmawati, Enni Suwarsi Rahayu
Lemongrass (Cymbopogon nardus (L.) Randle) plantlets are interesting to be used as tissue culture-based souvenir. The success of plantlet propagation through photomyxotrophic culture is influenced by several factors, including sucrose concentration and light intensity. The research aimed to analyze the effect of sucrose concentration and light intensity on the growth of lemongrass plantlets. This experimental research used randomized block factorial design with two factors, namely sucrose concentration (0 g/l, 10 g/l, 20 g/l, 30 g/l) and light intensity (2000 lux, 3000 lux, 4000 lux, 5000 lux). The variables observed were some parameters of growth and development of plantlet. The data were analyzed with two-way Analyses of Variance and Duncan Multiple Range Test. The results showed that sucrose concentration had significant effect on the number of shoots and leaves of plantlet. The light intensity and the interaction of both factors had also significant effect on the number of shoots and leaves. The most optimal treatments were sucrose of 20 g/l and light intensity of 3000 lux. The results of this study innovate the tissue culture technique using photomyxotrophic system in order to produce sterile lemongrass plantlets in large quantities.
{"title":"Development of Photomyxotrophic Culture Protocol of Lemongrass Through Sucrose Concentration Increase and Light Intensity Decrease","authors":"Desy Irmawati, Enni Suwarsi Rahayu","doi":"10.15294/biosaintifika.v15i2.38303","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i2.38303","url":null,"abstract":"Lemongrass (Cymbopogon nardus (L.) Randle) plantlets are interesting to be used as tissue culture-based souvenir. The success of plantlet propagation through photomyxotrophic culture is influenced by several factors, including sucrose concentration and light intensity. The research aimed to analyze the effect of sucrose concentration and light intensity on the growth of lemongrass plantlets. This experimental research used randomized block factorial design with two factors, namely sucrose concentration (0 g/l, 10 g/l, 20 g/l, 30 g/l) and light intensity (2000 lux, 3000 lux, 4000 lux, 5000 lux). The variables observed were some parameters of growth and development of plantlet. The data were analyzed with two-way Analyses of Variance and Duncan Multiple Range Test. The results showed that sucrose concentration had significant effect on the number of shoots and leaves of plantlet. The light intensity and the interaction of both factors had also significant effect on the number of shoots and leaves. The most optimal treatments were sucrose of 20 g/l and light intensity of 3000 lux. The results of this study innovate the tissue culture technique using photomyxotrophic system in order to produce sterile lemongrass plantlets in large quantities.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135163454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-01DOI: 10.15294/biosaintifika.v15i1.39696
S. Sukmaningrum, Dian Nasyitatul Jannah, A. Nuryanto
Greenback Mullets (Planiliza subviridis) are a commercial commodity and are fished daily in the Segara Anakan Cilacap, although has not been overfished. Continuous and unregulated fishing might harm the Greenback Mullets population. Therefore, sustainable fisheries of this species are still needed. Reproductive biology is among the essential aspect that supports sustainable fisheries. This research aims to obtain information on the reproductive biology of Greenback Mullet in Segara Anakan Cilacap. The research used a survey method with a purposive random sampling technique. The observed parameters were the gonad maturity stage, gonad maturity index, and fecundity. The data were analyzed descriptively. The female individuals have gonad maturity stages III and IV, gonad maturity stage was 16.81±2.59 and 16.92±2.51, and fecundity was 37,208-183,661 and 38,468 - 272,835 in August and October, respectively. Conversely, male individuals have a low gonad maturity stage and gonad maturity index. Based on the data, Greenback Mullets in the Segara Anakan estuary have maximum gonad maturity stage, gonad maturity index, and fecundity in August and October, while in February they were in early gonadal development. These data are essential for sustainable fisheries management in the Segara Anakan estuary Cilacap, Central Java, especially for Planiliza subviridis.
{"title":"Reproductive Biology of Greenback Mullet, Planiliza subviridis (Valenciennes, 1836) from Segara Anakan Cilacap","authors":"S. Sukmaningrum, Dian Nasyitatul Jannah, A. Nuryanto","doi":"10.15294/biosaintifika.v15i1.39696","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.39696","url":null,"abstract":"Greenback Mullets (Planiliza subviridis) are a commercial commodity and are fished daily in the Segara Anakan Cilacap, although has not been overfished. Continuous and unregulated fishing might harm the Greenback Mullets population. Therefore, sustainable fisheries of this species are still needed. Reproductive biology is among the essential aspect that supports sustainable fisheries. This research aims to obtain information on the reproductive biology of Greenback Mullet in Segara Anakan Cilacap. The research used a survey method with a purposive random sampling technique. The observed parameters were the gonad maturity stage, gonad maturity index, and fecundity. The data were analyzed descriptively. The female individuals have gonad maturity stages III and IV, gonad maturity stage was 16.81±2.59 and 16.92±2.51, and fecundity was 37,208-183,661 and 38,468 - 272,835 in August and October, respectively. Conversely, male individuals have a low gonad maturity stage and gonad maturity index. Based on the data, Greenback Mullets in the Segara Anakan estuary have maximum gonad maturity stage, gonad maturity index, and fecundity in August and October, while in February they were in early gonadal development. These data are essential for sustainable fisheries management in the Segara Anakan estuary Cilacap, Central Java, especially for Planiliza subviridis.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78366326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-01DOI: 10.15294/biosaintifika.v15i1.41895
Fatona Nur, T. Erfi̇anti̇, Dea Putri Andeska, Renata Adaranyssa Egistha Putri, Istini Nurafifah, B. R. Sadewo, E. Suyono
Euglena sp. is green microalgae in an acidic environment (pH 2.5-3.5). Euglena sp. has recently been developed widely in industry because of its capability to produce lipids that can be utilized to synthesize biofuel. Microalgae is a potential source of biodiesel, especially in the form of a consortium culture. One of the microalgae consortium cultures that have been explored is the nature consortium microalgae of Glagah strain. The Glagah consortia were isolated from Lagoon in the Glagah Beach, Kulonprogo, Yogyakarta. This study aimed to determine the total production of biomass, lipids, carbohydrates, and proteins of mixed culture of Glagah strain consortium and Euglena sp. as biodiesel substrate. The biomass test was measured using the dry weigh method using a filtration vacuum pump kit, lipids were measured using the Blight Dryer method by adding chloroform and methanol as solvents, carbohydrates were measured using the Sulfur Phenol Acid method by adding Phenol and sulfuric acid (H2SO4), and proteins were measured using the Bradford method by adding SDS and Bradford’s solution. The total production and productivity of biomass, lipids, carbohydrates, and proteins showed that the mixed culture of Glagah strain consortium with Euglena sp. was higher than the Glagah strain consortium. It reached 0.410 g/L; 0.253; 0.856 g/L; and 0.623 g/L. Therefore, it could be concluded that the mixed culture of the Glagah strain consortium with Euglena sp. could increase the production of biomass, lipids, carbohydrates, and protein up to two times that of the Glagah strain consortium so that this mixed culture treatment could be used as a reference in microalgae cultivation for biodiesel.
{"title":"Enhancement of Microalgal Metabolite Production through Euglena sp. Local Strain and Glagah Strain Consortia","authors":"Fatona Nur, T. Erfi̇anti̇, Dea Putri Andeska, Renata Adaranyssa Egistha Putri, Istini Nurafifah, B. R. Sadewo, E. Suyono","doi":"10.15294/biosaintifika.v15i1.41895","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.41895","url":null,"abstract":"Euglena sp. is green microalgae in an acidic environment (pH 2.5-3.5). Euglena sp. has recently been developed widely in industry because of its capability to produce lipids that can be utilized to synthesize biofuel. Microalgae is a potential source of biodiesel, especially in the form of a consortium culture. One of the microalgae consortium cultures that have been explored is the nature consortium microalgae of Glagah strain. The Glagah consortia were isolated from Lagoon in the Glagah Beach, Kulonprogo, Yogyakarta. This study aimed to determine the total production of biomass, lipids, carbohydrates, and proteins of mixed culture of Glagah strain consortium and Euglena sp. as biodiesel substrate. The biomass test was measured using the dry weigh method using a filtration vacuum pump kit, lipids were measured using the Blight Dryer method by adding chloroform and methanol as solvents, carbohydrates were measured using the Sulfur Phenol Acid method by adding Phenol and sulfuric acid (H2SO4), and proteins were measured using the Bradford method by adding SDS and Bradford’s solution. The total production and productivity of biomass, lipids, carbohydrates, and proteins showed that the mixed culture of Glagah strain consortium with Euglena sp. was higher than the Glagah strain consortium. It reached 0.410 g/L; 0.253; 0.856 g/L; and 0.623 g/L. Therefore, it could be concluded that the mixed culture of the Glagah strain consortium with Euglena sp. could increase the production of biomass, lipids, carbohydrates, and protein up to two times that of the Glagah strain consortium so that this mixed culture treatment could be used as a reference in microalgae cultivation for biodiesel.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90141002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-01DOI: 10.15294/biosaintifika.v15i1.40871
Fadilla Hefzi, M. Mansyurdin, T. Maideliza
Ceiba pentandra (L.) Gaertn. is a plant whose fruit is used to produce fiber and seeds for biofuel, which has the potential to be developed in critical land because it is adaptive to grow in critical land that is less fertile and lacks water. In West Sumatra, several critical land locations are overgrown by C. pentandra plants. This study aims to determine the genetic variation of C. pentandra in five critical land populations in West Sumatra as the basis for selecting superior seeds for development in critical land. The research was conducted by the descriptive method using molecular data with the molecular marker RAPD (random amplified polymorphic DNA). The results showed that the primers OPA-01, OPA-02, and OPB-10 could detect polymorphisms. Pangkalan Koto Baru in the regency Lima Puluh Kota (H = 0.1212) was the population with the highest intrapopulation genetic variation value. Interpopulation genetic variation (DST = 0.0321) was lower than intrapopulation genetic variation (HS = 0.1021), with a low genetic differentiation value (GST = 0.2392) and a high gene flow value (Nm = 1.5894). The genetic variation of C. pentandra can be used to select the parent in plant breeding programs.
{"title":"Analysis of Genetic Variations of Ceiba pentandra (L.) Gaertn. on Several Critical Lands in West Sumatra Using RAPD Molecular Markers","authors":"Fadilla Hefzi, M. Mansyurdin, T. Maideliza","doi":"10.15294/biosaintifika.v15i1.40871","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40871","url":null,"abstract":"Ceiba pentandra (L.) Gaertn. is a plant whose fruit is used to produce fiber and seeds for biofuel, which has the potential to be developed in critical land because it is adaptive to grow in critical land that is less fertile and lacks water. In West Sumatra, several critical land locations are overgrown by C. pentandra plants. This study aims to determine the genetic variation of C. pentandra in five critical land populations in West Sumatra as the basis for selecting superior seeds for development in critical land. The research was conducted by the descriptive method using molecular data with the molecular marker RAPD (random amplified polymorphic DNA). The results showed that the primers OPA-01, OPA-02, and OPB-10 could detect polymorphisms. Pangkalan Koto Baru in the regency Lima Puluh Kota (H = 0.1212) was the population with the highest intrapopulation genetic variation value. Interpopulation genetic variation (DST = 0.0321) was lower than intrapopulation genetic variation (HS = 0.1021), with a low genetic differentiation value (GST = 0.2392) and a high gene flow value (Nm = 1.5894). The genetic variation of C. pentandra can be used to select the parent in plant breeding programs.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90160181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.40284
Wolly Candramila, Hayatul Fajri, A. N. Mardiyyaningsih, R. Firmansyah, Didi Akbar
Phylogenetic analysis for plants can be very helpful in determining species identification or taxonomic status morphologically. rbcL and matK are widely used as genetic markers in constructing seed plant phylogenies. Different identification and new collection during the re-inventory from the previous study were found in two and four types of rare fruits in Sibohe Forest, West Kalimantan. The six types include pisang karok (Musa sp.) and Kandis (Garcinia sp.) as well as nubik (Artocarpus sp.), tehengan (Artocarpus sp.), smallest arok (Ficus sp.), and amok (Alpinia sp.). This study aimed to analyze the phenetic relationship of six rare fruits from Kalimantan based on rbcL and matK genes. DNA samples were obtained from dried body parts of the previous study and amplified by PCR using both forward and reverse primers for rbcL and matK genes. Amplification was observed on electrophoresis gel for rbcL gene of nubik, tehengan, smallest arok, amok, and pisang karok, while only pisang karok in matK gene. However, matK gene sequence was also obtained for amok even though the band was not seen on the gel. Phylogenetic analysis using the two genes confirmed the morphological identification reported in the previous study, however, the use of matK as a single gene for taxonomic confirmation needs to be reconsidered. The information of rbcL and matK sequences of six rare fruits from West Kalimantan could be the first information for building the DNA barcodes of the rare fruits in West Kalimantan.
{"title":"Taxonomic Confirmation of Rare Fruits in West Kalimantan Using rbcL and matK Sequences","authors":"Wolly Candramila, Hayatul Fajri, A. N. Mardiyyaningsih, R. Firmansyah, Didi Akbar","doi":"10.15294/biosaintifika.v15i1.40284","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.40284","url":null,"abstract":"Phylogenetic analysis for plants can be very helpful in determining species identification or taxonomic status morphologically. rbcL and matK are widely used as genetic markers in constructing seed plant phylogenies. Different identification and new collection during the re-inventory from the previous study were found in two and four types of rare fruits in Sibohe Forest, West Kalimantan. The six types include pisang karok (Musa sp.) and Kandis (Garcinia sp.) as well as nubik (Artocarpus sp.), tehengan (Artocarpus sp.), smallest arok (Ficus sp.), and amok (Alpinia sp.). This study aimed to analyze the phenetic relationship of six rare fruits from Kalimantan based on rbcL and matK genes. DNA samples were obtained from dried body parts of the previous study and amplified by PCR using both forward and reverse primers for rbcL and matK genes. Amplification was observed on electrophoresis gel for rbcL gene of nubik, tehengan, smallest arok, amok, and pisang karok, while only pisang karok in matK gene. However, matK gene sequence was also obtained for amok even though the band was not seen on the gel. Phylogenetic analysis using the two genes confirmed the morphological identification reported in the previous study, however, the use of matK as a single gene for taxonomic confirmation needs to be reconsidered. The information of rbcL and matK sequences of six rare fruits from West Kalimantan could be the first information for building the DNA barcodes of the rare fruits in West Kalimantan.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80323660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.39808
Nadya Audina N. S, M. Muniroh, N. Kusumaningrum, Farmaditya E P M, Renni Yuniati
Reversal reaction is a type IV cellular hypersensitivity reaction in leprosy. Interleukin-6 is a mediator with a pleiotropic effect on inflammation and immune response. Steroids are the standard therapy in the treatment of reaction reversal. Clitoria ternatea flower extract has anti-inflammatory properties. Long-term use of steroids can cause side effects. Therefore, it is necessary to give adjuvant therapy that can shorten the treatment period. This study aims to analyze the effect of adjuvant therapy of Clitoria ternatea extract to reduce IL-6 levels in reversal reaction. Experimental research design with pre and post-randomized single-blinded controlled trial, involving 22 subjects with reversal reaction. The control group received standard therapy and the treatment group received standard and adjuvant therapy with Clitoria ternatea extract. IL-6 levels were measured by ELISA. Data analysis used the Wilcoxon test and Independent T-test. The results showed a significant difference in treatment group (p=0.003) and control group (p=0.016). The mean decrease in serum IL-6 levels in treatment group was 81.35 pg/ml and in control group was 24.30 pg/ml (p=0.027). Clitoria ternatea showed a significant IL-6-lowering effect in patients with reversal reactions. This study has demonstrated the potential of Clitoria ternatea extract as adjuvant therapy in patients with leprosy reactions.
{"title":"Clitoria ternatea L. Extract as Adjuvant Therapy on Reducing IL–6 Levels in Reversal Reaction","authors":"Nadya Audina N. S, M. Muniroh, N. Kusumaningrum, Farmaditya E P M, Renni Yuniati","doi":"10.15294/biosaintifika.v15i1.39808","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.39808","url":null,"abstract":"Reversal reaction is a type IV cellular hypersensitivity reaction in leprosy. Interleukin-6 is a mediator with a pleiotropic effect on inflammation and immune response. Steroids are the standard therapy in the treatment of reaction reversal. Clitoria ternatea flower extract has anti-inflammatory properties. Long-term use of steroids can cause side effects. Therefore, it is necessary to give adjuvant therapy that can shorten the treatment period. This study aims to analyze the effect of adjuvant therapy of Clitoria ternatea extract to reduce IL-6 levels in reversal reaction. Experimental research design with pre and post-randomized single-blinded controlled trial, involving 22 subjects with reversal reaction. The control group received standard therapy and the treatment group received standard and adjuvant therapy with Clitoria ternatea extract. IL-6 levels were measured by ELISA. Data analysis used the Wilcoxon test and Independent T-test. The results showed a significant difference in treatment group (p=0.003) and control group (p=0.016). The mean decrease in serum IL-6 levels in treatment group was 81.35 pg/ml and in control group was 24.30 pg/ml (p=0.027). Clitoria ternatea showed a significant IL-6-lowering effect in patients with reversal reactions. This study has demonstrated the potential of Clitoria ternatea extract as adjuvant therapy in patients with leprosy reactions.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"334 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77601832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.15294/biosaintifika.v15i1.39899
S. Jannah, Resanti Adityani, T. R. Saraswati, S. Purwantisari
Guppy fish (Poecilia reticulata var. Mosaic) is a type of ornamental fish that has many enthusiasts. Guppy fish culture still has low growth and viability in guppy fish culture. The previous solution was to apply antibiotics, but as time goes by antibiotics cause bacterial resistance. Another solution is the application of probiotics to feed. L.paracasei is one of the lactic acid probiotic bacteria and has never been applied to guppies. This study aimed to analyze the effect of the probiotic on performance (growth and viability, gill and gut histology, and LAB (Lactic Acid Bacteria L.paracasei) total) in guppies. 180 fish were randomly assigned into four triplicates groups and there were four treatment groups: 0 (Control), 5, 10, and 15 mL. kg-1 feed. As the result of this research, the highest body weight and length growth performance is found in the P3 group. Meanwhile, there were no significant differences between the groups of guppies viability. Gill and intestinal histology showed that control group was the best group. The conclusion of this study is that 15 mL. kg-1 is considered the most effective for improving the performance of guppy fish. The addition of L.paracasei can be used as feed supplements to improve performance of guppy fish.
孔雀鱼(Poecilia reticulata var. Mosaic)是一种有许多爱好者的观赏鱼。在孔雀鱼养殖中，孔雀鱼的生长和生存能力仍然很低。以前的解决方案是使用抗生素，但随着时间的推移，抗生素会引起细菌耐药性。另一种解决方案是在饲料中应用益生菌。副干酪乳杆菌是乳酸菌中的一种，尚未应用于孔雀鱼。本研究旨在分析益生菌对孔雀鱼生产性能(生长和活力、鳃和肠道组织学以及副乳酸菌总数)的影响。将180尾鱼随机分为4个重复组，分别设0(对照)、5、10和15 ml kg-1饲料4个处理组。本研究结果表明，P3组的体重和体长生长性能最高。同时，各组间孔雀鱼的生存能力无显著差异。鳃和肠道组织学检查显示，对照组为最佳组。本研究的结论是，15ml . kg-1被认为是提高孔雀鱼性能最有效的剂量。在孔雀鱼饲料中添加副黄芪可以提高孔雀鱼的生产性能。
{"title":"The Effect of Probiotic Lactobacillus paracasei on The Performance of Guppy Fish (Poecilia reticulata var. Mosaic)","authors":"S. Jannah, Resanti Adityani, T. R. Saraswati, S. Purwantisari","doi":"10.15294/biosaintifika.v15i1.39899","DOIUrl":"https://doi.org/10.15294/biosaintifika.v15i1.39899","url":null,"abstract":"Guppy fish (Poecilia reticulata var. Mosaic) is a type of ornamental fish that has many enthusiasts. Guppy fish culture still has low growth and viability in guppy fish culture. The previous solution was to apply antibiotics, but as time goes by antibiotics cause bacterial resistance. Another solution is the application of probiotics to feed. L.paracasei is one of the lactic acid probiotic bacteria and has never been applied to guppies. This study aimed to analyze the effect of the probiotic on performance (growth and viability, gill and gut histology, and LAB (Lactic Acid Bacteria L.paracasei) total) in guppies. 180 fish were randomly assigned into four triplicates groups and there were four treatment groups: 0 (Control), 5, 10, and 15 mL. kg-1 feed. As the result of this research, the highest body weight and length growth performance is found in the P3 group. Meanwhile, there were no significant differences between the groups of guppies viability. Gill and intestinal histology showed that control group was the best group. The conclusion of this study is that 15 mL. kg-1 is considered the most effective for improving the performance of guppy fish. The addition of L.paracasei can be used as feed supplements to improve performance of guppy fish.","PeriodicalId":30622,"journal":{"name":"Biosaintifika Journal of Biology Biology Education","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82025494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：