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DISINFECTANT APPLICATION LEVELS IN COWSHED FOR PREVENTING OF FOOT AND MOUTH DISEASES IN GIANYAR 吉安亚尔地区为预防口蹄疫而在牛舍中使用消毒剂的水平
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p21
Ni Ketut Vonny, I. K. Suada, I. W. M. Tenaya
Foot and mouth disease (FMD) is a contagious viral disease that attacks cloven-hoofed livestock such as cattle, buffalo, sheep and goats. The spread of FMD in cattle is prone to occur, therefore efforts are needed to prevent transmission and spread of the disease, one of which is through biosecurity management, namely sanitation and disinfection. The aim of this research is to determine the level of implementation of disinfection in cattle pens and its relationship to preventing FMD cases in cattle in Gianyar Regency, Bali. The data collection method used a questionnaire by conducting interviews with one member of each cattle breeder group consisting of simantri and non-simantri breeder groups in Gianyar Regency. The results of this study show that the level of application of disinfection in preventing FMD by cattle breeders in Gianyar Regency is classified as poor (score ≤ 60%) by 76.7% of breeders, is classified as sufficient (score 60-75%) by 23.3% of breeders, and is classified as good (score ≥ 76%) as many as 0% of breeders. Meanwhile, no relationship was found between the level of disinfectant application and the prevention of FMD cases. It was concluded that cattle breeders in Gianyar Regency had a poor level of disinfection implementation and prevention of FMD transmission had been carried out through vaccination which had an effect on reducing cases. However, implementing disinfection is also important to prevent the possibility of livestock being infected by other infectious diseases. Therefore, breeders are expected to implement biosecurity, namely continuous disinfection to prevent the possibility of infection from other diseases on the farm.
口蹄疫(FMD)是一种传染性病毒疾病,主要侵害牛、水牛、绵羊和山羊等蹄类动物。口蹄疫很容易在牛群中传播,因此需要努力防止该疾病的传播和扩散,其中之一就是通过生物安全管理,即卫生和消毒。本研究旨在确定巴厘岛吉尼亚尔县牛栏消毒的实施水平及其与预防牛口蹄疫病例的关系。数据收集方法采用问卷调查的方式,对吉安亚尔地区由模拟和非模拟饲养牛群组成的每个饲养牛群的一名成员进行访谈。研究结果表明,76.7% 的饲养者将吉尼亚尔省养牛者在预防口蹄疫方面的消毒应用水平归类为较差(得分≤ 60%),23.3% 的饲养者将其归类为足够(得分 60-75%),还有 0% 的饲养者将其归类为良好(得分≥ 76%)。同时,没有发现消毒剂应用水平与口蹄疫病例预防之间有任何关系。由此得出结论,吉尼亚尔地区的养牛户消毒水平较低,通过接种疫苗来预防口蹄疫传播,这对减少病例有一定作用。然而,实施消毒对于防止牲畜感染其他传染病也很重要。因此,饲养者应实施生物安全措施,即持续消毒,以防止农场感染其他疾病的可能性。
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引用次数: 0
SHEDDING DETECTION OF NEWCASTLE DISEASE VIRUS VACCINE IN LAYING HENS POST-VACCINATION 蛋鸡接种新城疫病毒疫苗后的脱落检测
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p19
Ni Kadek Kamala Dewi, G. A. Y. Kencana, Ida Bagus Kade Suardana
The poultry industry in Indonesia is still experiencing problems, especially in terms of disease attacks, one of which is Newcastle Disease (ND). ND is caused by Avian Paramyxovirus type-1 (APMV-1) virus and is an infectious and also acute disease in Indonesia. ND management has so far been carried out by vaccination and improved husbandry management. ND vaccination can use active vaccines, inactive vaccines or combination vaccines. Despite vaccination, ND is still frequently reported.  The aim of this study was to determine the safety of Newcastle Disease (ND) Genotype VII vaccine based on post-vaccination virus shedding. Shedding viruses leave the body of poultry through excretion, especially feces, which can cause environmental pollution. The samples used were 10 cloacal swab samples of laying hens taken for five periods and isolation of embryonated chicken eggs. The inoculated liquid on embryonated chicken eggs was harvested and HA test was conducted to check the shedding of ND vaccine virus. The results showed no shedding of the vaccine virus characterized by negative results in the HA test. These negative results indicate that the inactivated ND Genotype VII vaccine is safe for the environment, however farmers need to implement biosecurity to prevent the entry of ND into the farm.
印度尼西亚的家禽业仍然面临着各种问题,尤其是疾病的侵袭,其中之一就是新城疫(ND)。新城疫由禽副粘病毒 1 型(APMV-1)病毒引起,在印尼是一种传染性疾病,也是一种急性疾病。迄今为止,ND 的管理主要通过疫苗接种和改善饲养管理来实现。ND 疫苗接种可使用活性疫苗、非活性疫苗或混合疫苗。尽管接种了疫苗,但仍经常有 ND 报告。 本研究的目的是根据接种后的病毒脱落情况确定新城疫(ND)基因型 VII 疫苗的安全性。脱落的病毒会通过排泄物,尤其是粪便离开家禽的身体,从而造成环境污染。使用的样本是 10 份产蛋鸡泄殖腔拭子样本,共采集了 5 个时期,并分离了鸡胚蛋。采集鸡胚蛋上的接种液并进行 HA 测试,以检查 ND 疫苗病毒的脱落情况。结果显示,疫苗病毒没有脱落,HA 试验结果为阴性。这些阴性结果表明,ND 基因 VII 型灭活疫苗对环境是安全的,但农民需要实施生物安全措施,以防止 ND 进入农场。
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引用次数: 0
SEROPREVALENCE AND RISK FACTORS OF SURRA INCIDENT IN HORSES AND CATTLES IN EAST SUMBA DISTRICT 东苏姆巴地区马和牛的血清流行率和苏拉病毒感染的风险因素
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p13
Rambu Peristiwati Pandjukang, I. Apsari, April Hari Wardhana, N. S. Dharmawan
Surra caused by Trypanosoma evansi is a disease that appears every year in East Sumba district and causes quite large losses. This study aims to determine the seroprevalence and risk factors that influence the incidence of surra in East Sumba district using a total of 226 horse and cattle blood samples obtained from seven sub-districts and the examination was carried out using the serological method, namely the card agglutination test (CATT/ T. evansi). A semi-structured questionnaire was used to collect data on risk factors associated with surra. The results showed that the seroprevalence of surra in horses and cattle was 32,30%. Seroprevalence in horses was 32,85% and in cattle was 26,31%. The risk factor of presence of flies and maintenance methods statistically have a significant effect on the incidence of surra, while age, gender, the presence of other animals and the farmer's knowledge about surra do not statistically have a significant effect on the incidence of surra. Thus, it is necessary to carry out surra surveillance in cattle which are the reservoirs and preventive measures are needed, especially those related to risk factors wich statistically have a significant effect on the incidence of surra. Apart from that, it is necessary to carry out further research on other risk factors related to the incidence of surra and research in different season periods.
由 evansi 锥虫引起的苏拉病每年都会在东松巴地区出现,并造成相当大的损失。这项研究旨在确定东松巴地区的血清流行率和影响苏拉病发病率的风险因素,研究使用了从七个分区获得的共计 226 份马和牛血液样本,并使用血清学方法,即卡片凝集试验(CATT/T. evansi)进行了检测。采用半结构式问卷收集了与苏拉相关的风险因素的数据。结果显示,马和牛的血清阳性率为32.30%。马的血清阳性率为 32.85%,牛为 26.31%。据统计,苍蝇的存在和维护方法等风险因素对糜烂的发病率有显著影响,而年龄、性别、其他动物的存在和农场主对糜烂的了解程度对糜烂的发病率没有显著影响。因此,有必要对作为疫源地的牛群进行沙拉监测,并采取预防措施,尤其是与那些在统计学上对沙拉发病率有显著影响的风险因素有关的措施。除此以外,有必要进一步研究与囊肿疽发病率有关的其他风险因素,并在不同季节开展研究。
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引用次数: 0
HISTOPATHOLOGY OF WHITE RAT SPLEEN INDUCED BY THE APPLICATION OF MIMOSIN FROM LEUCAENA LEAF SIMPLISIA 应用白花蛇舌草叶简中的含羞草素诱导的白鼠脾脏组织病理学研究
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p18
Luh Gede Tasya Pradnya Prastistha, I. K. Berata, N. S. Dharmawan, N. Susari, N. L. E. Setiasih, L. M. Sudimartini
The Leucaena leucocephala is often used as an alternative feed for cattle. However, the utilization of leucaena leaves as cattle feed needs to be restricted because leucaena contains an anti-nutritional substance called mimosine. This study aimed to evaluate the impact of mimosine exposure from the leucaena plant on the spleen of white rats. The research used twenty male Wistar strain white rats aged 2 months weighing 300–350 grams, with spleen tissue as the sample. The treatment was divided into four different groups, including a negative control group, a positive control group given standard mimosine, and two groups exposed to leucaena leaf simplisia at different doses for 14 days via gavage. On the 15th day, the rats were euthanized for spleen extraction to prepare histopathological slides stained with hematoxylin-eosin. The results of histopathological examination, including congestion, bleeding, and inflammation, were analyzed using non-parametric Kruskal-Wallis and Mann-Whitney tests. Oral administration of mimosine from leucaena leaf simplisia to the spleen of white rats showed histopathological changes such as congestion, bleeding, and inflammation. The Kruskal-Wallis test revealed significant changes in congestion, bleeding, and inflammation. The Mann-Whitney test between rats given mimosine from leucaena leaf simplisia at doses of 50 mg/head/day and 150 mg/head/day showed a significant effect on inflammatory histopathological changes. Based on the research results, it can be concluded that there were histopathological changes, including congestion, bleeding, and inflammation due to mimosine administration from leucaena leaf simplisia compared to controls, and there was an influence of different doses of mimosine from leucaena leaf simplisia between doses of 50 mg/head/day and 150 mg/head/day, especially on inflammatory lesions. It is recommended for future research to further investigate the administration of mimosine from leucaena leaf simplisia over a longer period with larger doses.
白千层叶常被用作牛的替代饲料。然而,由于白千层含有一种名为 "含羞草碱 "的抗营养物质,因此将白千层叶用作牛饲料需要受到限制。本研究旨在评估白芒花植物含羞草碱对白鼠脾脏的影响。研究使用了 20 只年龄为 2 个月,体重为 300-350 克的雄性 Wistar 株白鼠,以脾脏组织为样本。治疗分为四个不同的组别,包括阴性对照组、给予标准含羞草碱的阳性对照组,以及通过灌胃暴露于不同剂量的白头翁叶简体14天的两组。第 15 天,对大鼠实施安乐死,提取脾脏并制作组织病理学切片,用苏木精-伊红染色。组织病理学检查的结果,包括充血、出血和炎症,采用非参数 Kruskal-Wallis 检验和 Mann-Whitney 检验进行分析。给白鼠脾脏口服白花蛇舌草叶简中的含羞草碱后,会出现充血、出血和炎症等组织病理学变化。Kruskal-Wallis 检验显示,充血、出血和炎症有显著变化。按 50 毫克/头/天和 150 毫克/头/天的剂量给大白鼠服用白头翁叶简中的含羞草碱,两者之间的 Mann-Whitney 检验显示,含羞草碱对炎症组织病理学变化有显著影响。根据研究结果,可以得出结论:与对照组相比,服用白头翁叶中的含羞草碱会引起组织病理变化,包括充血、出血和炎症,而且在 50 毫克/头/天和 150 毫克/头/天的剂量之间,不同剂量的白头翁叶中的含羞草碱会产生影响,尤其是对炎症病变的影响。建议在今后的研究中进一步研究如何以更大的剂量长期服用白茅叶中的含羞草碱。
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引用次数: 0
HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE 本地巴厘鸭生长期右心室的组织形态测量
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p20
Delima Marsinta Ida Pasaribu, N. L. E. Setiasih, L. Heryani, I. K. Berata, N. Susari, I. K. Suatha
Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.
心脏是血液循环的主要器官,起着将血液泵送到所有体细胞并返回心脏的作用。鸟类心脏有四个心腔,包括两个心房(前心房和后心房)和两个心室(前心室和后心室),每个心腔有三层心壁,分别由心外膜、心肌和心内膜组成。本研究旨在获得巴厘鸭生长期心脏的组织学结构和心室的组织形态计量学信息。研究样本为 18 只巴厘鸭,分为两个性别组,每组 9 只(3 个月大)。右心室样本经血栓素-伊红(HE)染色处理后制成组织学制剂。解剖学和组织学结果采用描述性定性分析,而组织形态计量学采用SPSS 26程序的anova检验和平均估算。男性心外膜、心肌和心内膜的平均厚度分别为 43,11 ± 12,05 微米、1.145,19 ± 133,99 微米、23,41 ± 6,91 微米。女性分别为 34,98 ± 17,03 微米、1.367,41 ± 412,43 微米、11,86 ± 5,06 微米。组织形态计量学数据分析显示,心内膜厚度的测量结果存在显著差异(P0,05)。由此可以得出结论,巴厘岛鸭心脏的解剖结构和心室的组织学结构在雌雄之间没有差异,但心内膜层的组织形态测量存在差异。目前对巴厘岛鸭心脏的研究还很少,因此还需要进一步研究巴厘岛鸭不同阶段的心外膜层、心肌层、心内膜层或其他心腔的结构和组织形态学。
{"title":"HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE","authors":"Delima Marsinta Ida Pasaribu, N. L. E. Setiasih, L. Heryani, I. K. Berata, N. Susari, I. K. Suatha","doi":"10.24843/bulvet.2024.v16.i02.p20","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p20","url":null,"abstract":"Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"39 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A STRUCTURE AND HISTOMORPHOMETRY OF BALI DUCK TESTES IN THE GROWER PHASE 巴厘鸭生长期睾丸的结构和组织形态学
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p17
Ni Made Santi Rahayu Adiari, N. L. E. Setiasih, I. P. Sampurna, N. K. Suwiti, N. Susari, I. K. Suatha
Testes are male reproductive organs which are responsible for producing spermatozoa and steroid hormones. This study aims to determine the histology and histomorphometry structure of bali duck testes in the grower phase at the age of 12 weeks in one of the farms in Mengwi District, Badung Regency. The number of samples used in this research was 10 male bali ducks that were in good health. There are two examination methods carried out, namely the qualitative descriptive anatomical structure and histology examination method and the quantitative histomorphometry examination method of bali duck testicles which are analyzed using the One-Sample T-test with a confidence level of 95%. The results of this study show the anatomical structure of the bali duck testicles which are located in the abdominal cavity, between the kidneys and the lungs. The pair of testicles have the shape of a bean and are cream colored. The results of research on the histological structure of bali duck testes consist of seminiferous tubules, interstitial tissue, leydig cells, and tunica albuginea. The results of histomorphometry measurements of bali duck testes showed that the wall thickness of the seminiferous tubules was 23.6760 ± 1.4425 (µm), the number of seminiferous tubules was 421.1000 ± 56.1396 and the thickness of the tunica albuginea was 29.5000 ± 5.048 (µm). Considering the importance of knowledge about the structure of bali duck testicles, it is recommended for future researchers to examine the structure and histomorphometry of bali duck testicles at different ages in the growing phase.
睾丸是雄性生殖器官,负责产生精子和类固醇激素。本研究旨在确定巴东县 Mengwi 区一家养殖场中 12 周龄生长期巴利鸭睾丸的组织学和组织形态学结构。本研究使用的样本数量为 10 只健康状况良好的雄性巴厘鸭。研究采用了两种检查方法,即巴厘鸭睾丸解剖结构和组织学定性描述检查法和组织形态学定量检查法,并使用置信度为 95% 的单样本 T 检验进行分析。研究结果显示了巴厘鸭睾丸的解剖结构,睾丸位于腹腔内肾脏和肺脏之间。这对睾丸呈豆状,乳白色。对巴厘鸭睾丸组织结构的研究结果表明,睾丸由曲细精管、间质组织、白细胞和白膜组成。巴厘鸭睾丸组织形态测量结果显示,曲细精管壁厚为 23.6760 ± 1.4425(微米),曲细精管数目为 421.1000 ± 56.1396,白膜厚度为 29.5000 ± 5.048(微米)。考虑到了解巴厘鸭睾丸结构的重要性,建议未来的研究人员研究生长阶段不同年龄巴厘鸭睾丸的结构和组织形态学。
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引用次数: 0
STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE 生长期巴厘鸭胰腺的结构和形态测量
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p12
I. G. A. P. Pratama, N. L. E. Setiasih, Sri Kayati Widyastuti, N. K. Suwiti, N. Susari, I. K. Suatha
The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry
胰腺是消化系统的一部分,起着消化辅助器官的作用。胰腺有两种功能,即外分泌和内分泌。本研究旨在确定不同性别的巴厘鸭(Anas Sp)在生长阶段的胰腺结构和形态。本研究使用了 18 只巴厘岛鸭,将其分为两组,即雌雄各 9 只(3 月龄)。解剖结构的检查方法是使用双目光学显微镜直接观察和组织学结构。形态测量使用卡尺测量长度和宽度,天平测量胰腺重量,量杯测量体积。组织形态测量使用奥林巴斯细胞传感器标准应用软件进行测量。解剖学和组织学结构数据的结果采用定性描述分析法进行分析,而形态计量数据则采用独立样本 T 检验法进行分析。巴厘岛鸭胰腺的解剖结构扁平,像一个分叶的舌头,呈粉红色,由囊、朗格汉斯小体、盲肠、闰管、小叶内管、小叶间管、小叶内隔、小叶间隔、静脉和动脉组成。巴厘岛鸭胰腺的形态测量结果表明,雌雄鸭胰腺的形态差异显著(P0.05)。雌雄巴厘岛鸭胰腺的解剖结构和组织学相同,但在形态测量方面存在差异
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引用次数: 0
POTENTIAL OF MENIRAN LEAF EXTRACT ON NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS 美尼兰叶提取物对肉鸡新城疫抗体滴度的潜在影响
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p15
Citra Widiawati, Ida Bagus Kade Suardana, G. A. Y. Kencana, N. L. E. Setiasih, T. S. Nindhia, A. A. S. Kendran
Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.
新城疫(ND)是容易侵袭肉鸡的传染病之一。为提高鸡的免疫力而进行的疫苗接种往往效果不佳,因此需要绿膜兰(Phyllanthus niruri Linn.)作为免疫增强剂。绿毛番荔枝叶提取物的主要成分是黄酮类化合物,可触发 T 细胞帮助 B 细胞产生抗体。本研究旨在确定青钱柳叶提取物(Phyllanthus niruri Linn.)对加强免疫肉鸡 ND 抗体滴度的影响。研究设计采用了完全随机设计的嵌套模式,共有 30 只肉鸡被分为三个处理组,即 10 只肉鸡未接种玖玖拉索塔加强免疫疫苗,也未接种月桂叶提取物,但接种了安慰剂(K-);10 只肉鸡接种了玖玖拉索塔加强免疫疫苗,但未接种月桂叶提取物(K+);10 只肉鸡接种了玖玖拉索塔加强免疫疫苗,并在接种前 7 天和接种后 14 天在饮用水中添加了月桂叶提取物(P)。使用的样本分别为加强免疫前一天(9 日龄肉鸡)、加强免疫后一周(17 日龄肉鸡)和加强免疫后两周(24 日龄肉鸡)。获得的血清通过血凝抑制(HI)试验进行血清学检验。获得的数据使用 SPSS(统计产品和服务解决方案)软件进行统计分析(显著性水平为 5%)和回归分析。结果表明,在接种 ND 加强免疫后施用 meniran 叶提取物(Phyllanthus niruri Linn.)和取样时间的影响会增加 ND 抗体滴度,P 处理的平均值为 2.8、4.3、7.1,总平均值为 4.7(滴度以 HI log 2 表示),差异显著(P < 0.05)。回归分析表明,与 K- 和 K+ 处理相比,P 处理在 ND 强毒疫苗接种后第三周的抗体滴度增幅最大。
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引用次数: 0
THE CAUSATIVE FACTOR DETECTION AND IDENTIFICATION OF BOOPHILUS SP. INFESTATION IN BALI CATTLE IN BARRU REGENCY, SOUTH SULAWESI 南苏拉威省巴鲁县巴厘牛嗜血杆菌感染的致病因素检测与鉴定南苏拉威西省巴鲁县巴厘牛的嗜血杆菌感染情况
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p16
Suparmin Yuliani, Risha Catra Pradhany, Faizal Zakariya
In tropical climates such as Indonesia, the presence of ectoparasites occurs almost annually, which becomes one of the major problems due to ectoparasite infestations on ruminant farms. One problem occurred is the Boophilus sp. This study aimed to detect and identify causative factor in infestation of Boophilus sp. and correlation between tick infestation and incidence of blood parasite in Barru Regency. The study unit was the Bali cattle breeders spreaded in Mallusetasi District, Barru Regency. This study used a cross-sectional study to identify and determine the relationship between the causative factors of maintenance management and stock farmer knowledge level on the application of maintenance management in Bali cattle farms. The tabulated data of the maintenance management in Bali cattle breeding and breeder knowledge level causative factors against the tick ectoparasite infestation were descriptively analyzed and tested with a chi-square (χ2) method to measure the relationship of these factors and Boophilus sp. tick infestation at 95% of confidence level. The magnitude of relationship strength was calculated by the odd ratio (OR) test at a 95% of confidence level. Boophilus sp. tick ectoparasite infestation occurred in Bali cattle farms in Mallusetasi District, Barru Regency with an incidence rate of 68,8%. The causative factors that influence the incidence of tick infestations included the farmer educational level, breeder experience, number of maintenance, maintenance pattern, poor cage condition, and breeder's knowledge. The infestation of Boophilus sp. tick had a very significant correlation on the incidence of blood parasitic diseases. Therefore, to reduce tick infestation and the incidence of blood parasitic disease, planning of integrated control measures to create awareness about the importance and control of tick for livestock farmers is required.
在印度尼西亚等热带气候地区,几乎每年都会出现体外寄生虫,这成为反刍动物养殖场体外寄生虫侵扰的主要问题之一。本研究旨在检测和确定巴鲁地区嗜血鲣鸟感染的致病因素以及蜱虫感染与血液寄生虫发病率之间的相关性。研究单位为巴鲁行政区 Mallusetasi 县的巴厘岛养牛户。本研究采用横断面研究,以确定维护管理的致病因素与饲养者对巴厘岛养牛场维护管理应用的知识水平之间的关系。对巴厘岛养牛场维护管理和饲养员知识水平与蜱外寄生虫侵扰的致病因素的表格数据进行了描述性分析,并用卡方(χ2)方法进行了检验,以衡量这些因素与嗜血蜱侵扰的关系,置信度为 95%。在 95% 的置信水平下,关系强度的大小通过奇数比(OR)检验来计算。嗜血蜱是巴厘岛巴鲁行政区 Mallusetasi 县巴厘岛养牛场的体外寄生虫,发病率为 68.8%。影响蜱虫侵扰发生率的致病因素包括农场主的教育水平、饲养员的经验、维护次数、维护模式、笼舍条件差以及饲养员的知识水平。嗜血蜱的侵扰与血液寄生虫病的发病率有非常显著的相关性。因此,为了减少蜱虫的侵扰和血液寄生虫病的发病率,需要规划综合控制措施,使家畜饲养者认识到蜱虫的重要性和控制措施。
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引用次数: 0
HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE 生长期巴厘鸭胸肌浅层和胫骨肌肉的组织形态测量
Pub Date : 2024-04-05 DOI: 10.24843/bulvet.2024.v16.i02.p11
I. K. A. S. Prayoga, N. L. E. Setiasih, L. Heryani, N. K. Suwiti, N. Susari, I. K. Suatha
Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different.  Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
浅胸肌是位于体表的胸肌,起着翅膀运动的作用。胫骨头盖肌是小腿肌肉结构中的顶部肌肉,具有支撑鸟类身体的功能。本研究旨在测定生长期雌雄巴厘岛鸭胸肌表层和胫骨颅骨肌的组织形态学。本研究使用了 20 只 12 周龄的巴厘岛鸭。用双目光学显微镜直接进行解剖检查和组织学结构检查。使用奥林巴斯 Cellsens Standard 应用程序测量组织形态学。解剖学和组织学结果采用描述性定性分析,组织形态测量采用方差分析检验,并估计平均值。胸肌浅层和胫骨颅骨肌的组织学结构由肌纤维、筋膜束、内膜、周膜和外膜结缔组织组成。不同性别的筋膜大小、韧带周围结缔组织厚度和胸浅肌内膜的组织形态学差异显著(P0.05)。由此可以得出结论,处于生长期的男性和女性的胸浅肌和胫骨颅骨肌在解剖结构上是相同的,但组织学结构的大小不同。 雄性和雌性巴厘鸭胸肌浅层的组织形态有显著差异(P<0.05),但胫骨颅骨肌没有显著差异。关于巴厘岛鸭在其他年龄段的肌肉,还需要进一步研究。
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引用次数: 0
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Buletin Veteriner Udayana
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