Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p21
Ni Ketut Vonny, I. K. Suada, I. W. M. Tenaya
Foot and mouth disease (FMD) is a contagious viral disease that attacks cloven-hoofed livestock such as cattle, buffalo, sheep and goats. The spread of FMD in cattle is prone to occur, therefore efforts are needed to prevent transmission and spread of the disease, one of which is through biosecurity management, namely sanitation and disinfection. The aim of this research is to determine the level of implementation of disinfection in cattle pens and its relationship to preventing FMD cases in cattle in Gianyar Regency, Bali. The data collection method used a questionnaire by conducting interviews with one member of each cattle breeder group consisting of simantri and non-simantri breeder groups in Gianyar Regency. The results of this study show that the level of application of disinfection in preventing FMD by cattle breeders in Gianyar Regency is classified as poor (score ≤ 60%) by 76.7% of breeders, is classified as sufficient (score 60-75%) by 23.3% of breeders, and is classified as good (score ≥ 76%) as many as 0% of breeders. Meanwhile, no relationship was found between the level of disinfectant application and the prevention of FMD cases. It was concluded that cattle breeders in Gianyar Regency had a poor level of disinfection implementation and prevention of FMD transmission had been carried out through vaccination which had an effect on reducing cases. However, implementing disinfection is also important to prevent the possibility of livestock being infected by other infectious diseases. Therefore, breeders are expected to implement biosecurity, namely continuous disinfection to prevent the possibility of infection from other diseases on the farm.
{"title":"DISINFECTANT APPLICATION LEVELS IN COWSHED FOR PREVENTING OF FOOT AND MOUTH DISEASES IN GIANYAR","authors":"Ni Ketut Vonny, I. K. Suada, I. W. M. Tenaya","doi":"10.24843/bulvet.2024.v16.i02.p21","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p21","url":null,"abstract":"Foot and mouth disease (FMD) is a contagious viral disease that attacks cloven-hoofed livestock such as cattle, buffalo, sheep and goats. The spread of FMD in cattle is prone to occur, therefore efforts are needed to prevent transmission and spread of the disease, one of which is through biosecurity management, namely sanitation and disinfection. The aim of this research is to determine the level of implementation of disinfection in cattle pens and its relationship to preventing FMD cases in cattle in Gianyar Regency, Bali. The data collection method used a questionnaire by conducting interviews with one member of each cattle breeder group consisting of simantri and non-simantri breeder groups in Gianyar Regency. The results of this study show that the level of application of disinfection in preventing FMD by cattle breeders in Gianyar Regency is classified as poor (score ≤ 60%) by 76.7% of breeders, is classified as sufficient (score 60-75%) by 23.3% of breeders, and is classified as good (score ≥ 76%) as many as 0% of breeders. Meanwhile, no relationship was found between the level of disinfectant application and the prevention of FMD cases. It was concluded that cattle breeders in Gianyar Regency had a poor level of disinfection implementation and prevention of FMD transmission had been carried out through vaccination which had an effect on reducing cases. However, implementing disinfection is also important to prevent the possibility of livestock being infected by other infectious diseases. Therefore, breeders are expected to implement biosecurity, namely continuous disinfection to prevent the possibility of infection from other diseases on the farm.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140739948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p19
Ni Kadek Kamala Dewi, G. A. Y. Kencana, Ida Bagus Kade Suardana
The poultry industry in Indonesia is still experiencing problems, especially in terms of disease attacks, one of which is Newcastle Disease (ND). ND is caused by Avian Paramyxovirus type-1 (APMV-1) virus and is an infectious and also acute disease in Indonesia. ND management has so far been carried out by vaccination and improved husbandry management. ND vaccination can use active vaccines, inactive vaccines or combination vaccines. Despite vaccination, ND is still frequently reported. The aim of this study was to determine the safety of Newcastle Disease (ND) Genotype VII vaccine based on post-vaccination virus shedding. Shedding viruses leave the body of poultry through excretion, especially feces, which can cause environmental pollution. The samples used were 10 cloacal swab samples of laying hens taken for five periods and isolation of embryonated chicken eggs. The inoculated liquid on embryonated chicken eggs was harvested and HA test was conducted to check the shedding of ND vaccine virus. The results showed no shedding of the vaccine virus characterized by negative results in the HA test. These negative results indicate that the inactivated ND Genotype VII vaccine is safe for the environment, however farmers need to implement biosecurity to prevent the entry of ND into the farm.
印度尼西亚的家禽业仍然面临着各种问题,尤其是疾病的侵袭,其中之一就是新城疫(ND)。新城疫由禽副粘病毒 1 型(APMV-1)病毒引起,在印尼是一种传染性疾病,也是一种急性疾病。迄今为止,ND 的管理主要通过疫苗接种和改善饲养管理来实现。ND 疫苗接种可使用活性疫苗、非活性疫苗或混合疫苗。尽管接种了疫苗,但仍经常有 ND 报告。 本研究的目的是根据接种后的病毒脱落情况确定新城疫(ND)基因型 VII 疫苗的安全性。脱落的病毒会通过排泄物,尤其是粪便离开家禽的身体,从而造成环境污染。使用的样本是 10 份产蛋鸡泄殖腔拭子样本,共采集了 5 个时期,并分离了鸡胚蛋。采集鸡胚蛋上的接种液并进行 HA 测试,以检查 ND 疫苗病毒的脱落情况。结果显示,疫苗病毒没有脱落,HA 试验结果为阴性。这些阴性结果表明,ND 基因 VII 型灭活疫苗对环境是安全的,但农民需要实施生物安全措施,以防止 ND 进入农场。
{"title":"SHEDDING DETECTION OF NEWCASTLE DISEASE VIRUS VACCINE IN LAYING HENS POST-VACCINATION","authors":"Ni Kadek Kamala Dewi, G. A. Y. Kencana, Ida Bagus Kade Suardana","doi":"10.24843/bulvet.2024.v16.i02.p19","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p19","url":null,"abstract":"The poultry industry in Indonesia is still experiencing problems, especially in terms of disease attacks, one of which is Newcastle Disease (ND). ND is caused by Avian Paramyxovirus type-1 (APMV-1) virus and is an infectious and also acute disease in Indonesia. ND management has so far been carried out by vaccination and improved husbandry management. ND vaccination can use active vaccines, inactive vaccines or combination vaccines. Despite vaccination, ND is still frequently reported. The aim of this study was to determine the safety of Newcastle Disease (ND) Genotype VII vaccine based on post-vaccination virus shedding. Shedding viruses leave the body of poultry through excretion, especially feces, which can cause environmental pollution. The samples used were 10 cloacal swab samples of laying hens taken for five periods and isolation of embryonated chicken eggs. The inoculated liquid on embryonated chicken eggs was harvested and HA test was conducted to check the shedding of ND vaccine virus. The results showed no shedding of the vaccine virus characterized by negative results in the HA test. These negative results indicate that the inactivated ND Genotype VII vaccine is safe for the environment, however farmers need to implement biosecurity to prevent the entry of ND into the farm.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140739074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p13
Rambu Peristiwati Pandjukang, I. Apsari, April Hari Wardhana, N. S. Dharmawan
Surra caused by Trypanosoma evansi is a disease that appears every year in East Sumba district and causes quite large losses. This study aims to determine the seroprevalence and risk factors that influence the incidence of surra in East Sumba district using a total of 226 horse and cattle blood samples obtained from seven sub-districts and the examination was carried out using the serological method, namely the card agglutination test (CATT/ T. evansi). A semi-structured questionnaire was used to collect data on risk factors associated with surra. The results showed that the seroprevalence of surra in horses and cattle was 32,30%. Seroprevalence in horses was 32,85% and in cattle was 26,31%. The risk factor of presence of flies and maintenance methods statistically have a significant effect on the incidence of surra, while age, gender, the presence of other animals and the farmer's knowledge about surra do not statistically have a significant effect on the incidence of surra. Thus, it is necessary to carry out surra surveillance in cattle which are the reservoirs and preventive measures are needed, especially those related to risk factors wich statistically have a significant effect on the incidence of surra. Apart from that, it is necessary to carry out further research on other risk factors related to the incidence of surra and research in different season periods.
{"title":"SEROPREVALENCE AND RISK FACTORS OF SURRA INCIDENT IN HORSES AND CATTLES IN EAST SUMBA DISTRICT","authors":"Rambu Peristiwati Pandjukang, I. Apsari, April Hari Wardhana, N. S. Dharmawan","doi":"10.24843/bulvet.2024.v16.i02.p13","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p13","url":null,"abstract":"Surra caused by Trypanosoma evansi is a disease that appears every year in East Sumba district and causes quite large losses. This study aims to determine the seroprevalence and risk factors that influence the incidence of surra in East Sumba district using a total of 226 horse and cattle blood samples obtained from seven sub-districts and the examination was carried out using the serological method, namely the card agglutination test (CATT/ T. evansi). A semi-structured questionnaire was used to collect data on risk factors associated with surra. The results showed that the seroprevalence of surra in horses and cattle was 32,30%. Seroprevalence in horses was 32,85% and in cattle was 26,31%. The risk factor of presence of flies and maintenance methods statistically have a significant effect on the incidence of surra, while age, gender, the presence of other animals and the farmer's knowledge about surra do not statistically have a significant effect on the incidence of surra. Thus, it is necessary to carry out surra surveillance in cattle which are the reservoirs and preventive measures are needed, especially those related to risk factors wich statistically have a significant effect on the incidence of surra. Apart from that, it is necessary to carry out further research on other risk factors related to the incidence of surra and research in different season periods.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"103 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140735833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p18
Luh Gede Tasya Pradnya Prastistha, I. K. Berata, N. S. Dharmawan, N. Susari, N. L. E. Setiasih, L. M. Sudimartini
The Leucaena leucocephala is often used as an alternative feed for cattle. However, the utilization of leucaena leaves as cattle feed needs to be restricted because leucaena contains an anti-nutritional substance called mimosine. This study aimed to evaluate the impact of mimosine exposure from the leucaena plant on the spleen of white rats. The research used twenty male Wistar strain white rats aged 2 months weighing 300–350 grams, with spleen tissue as the sample. The treatment was divided into four different groups, including a negative control group, a positive control group given standard mimosine, and two groups exposed to leucaena leaf simplisia at different doses for 14 days via gavage. On the 15th day, the rats were euthanized for spleen extraction to prepare histopathological slides stained with hematoxylin-eosin. The results of histopathological examination, including congestion, bleeding, and inflammation, were analyzed using non-parametric Kruskal-Wallis and Mann-Whitney tests. Oral administration of mimosine from leucaena leaf simplisia to the spleen of white rats showed histopathological changes such as congestion, bleeding, and inflammation. The Kruskal-Wallis test revealed significant changes in congestion, bleeding, and inflammation. The Mann-Whitney test between rats given mimosine from leucaena leaf simplisia at doses of 50 mg/head/day and 150 mg/head/day showed a significant effect on inflammatory histopathological changes. Based on the research results, it can be concluded that there were histopathological changes, including congestion, bleeding, and inflammation due to mimosine administration from leucaena leaf simplisia compared to controls, and there was an influence of different doses of mimosine from leucaena leaf simplisia between doses of 50 mg/head/day and 150 mg/head/day, especially on inflammatory lesions. It is recommended for future research to further investigate the administration of mimosine from leucaena leaf simplisia over a longer period with larger doses.
{"title":"HISTOPATHOLOGY OF WHITE RAT SPLEEN INDUCED BY THE APPLICATION OF MIMOSIN FROM LEUCAENA LEAF SIMPLISIA","authors":"Luh Gede Tasya Pradnya Prastistha, I. K. Berata, N. S. Dharmawan, N. Susari, N. L. E. Setiasih, L. M. Sudimartini","doi":"10.24843/bulvet.2024.v16.i02.p18","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p18","url":null,"abstract":"The Leucaena leucocephala is often used as an alternative feed for cattle. However, the utilization of leucaena leaves as cattle feed needs to be restricted because leucaena contains an anti-nutritional substance called mimosine. This study aimed to evaluate the impact of mimosine exposure from the leucaena plant on the spleen of white rats. The research used twenty male Wistar strain white rats aged 2 months weighing 300–350 grams, with spleen tissue as the sample. The treatment was divided into four different groups, including a negative control group, a positive control group given standard mimosine, and two groups exposed to leucaena leaf simplisia at different doses for 14 days via gavage. On the 15th day, the rats were euthanized for spleen extraction to prepare histopathological slides stained with hematoxylin-eosin. The results of histopathological examination, including congestion, bleeding, and inflammation, were analyzed using non-parametric Kruskal-Wallis and Mann-Whitney tests. Oral administration of mimosine from leucaena leaf simplisia to the spleen of white rats showed histopathological changes such as congestion, bleeding, and inflammation. The Kruskal-Wallis test revealed significant changes in congestion, bleeding, and inflammation. The Mann-Whitney test between rats given mimosine from leucaena leaf simplisia at doses of 50 mg/head/day and 150 mg/head/day showed a significant effect on inflammatory histopathological changes. Based on the research results, it can be concluded that there were histopathological changes, including congestion, bleeding, and inflammation due to mimosine administration from leucaena leaf simplisia compared to controls, and there was an influence of different doses of mimosine from leucaena leaf simplisia between doses of 50 mg/head/day and 150 mg/head/day, especially on inflammatory lesions. It is recommended for future research to further investigate the administration of mimosine from leucaena leaf simplisia over a longer period with larger doses.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p20
Delima Marsinta Ida Pasaribu, N. L. E. Setiasih, L. Heryani, I. K. Berata, N. Susari, I. K. Suatha
Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.
{"title":"HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE","authors":"Delima Marsinta Ida Pasaribu, N. L. E. Setiasih, L. Heryani, I. K. Berata, N. Susari, I. K. Suatha","doi":"10.24843/bulvet.2024.v16.i02.p20","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p20","url":null,"abstract":"Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"39 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p17
Ni Made Santi Rahayu Adiari, N. L. E. Setiasih, I. P. Sampurna, N. K. Suwiti, N. Susari, I. K. Suatha
Testes are male reproductive organs which are responsible for producing spermatozoa and steroid hormones. This study aims to determine the histology and histomorphometry structure of bali duck testes in the grower phase at the age of 12 weeks in one of the farms in Mengwi District, Badung Regency. The number of samples used in this research was 10 male bali ducks that were in good health. There are two examination methods carried out, namely the qualitative descriptive anatomical structure and histology examination method and the quantitative histomorphometry examination method of bali duck testicles which are analyzed using the One-Sample T-test with a confidence level of 95%. The results of this study show the anatomical structure of the bali duck testicles which are located in the abdominal cavity, between the kidneys and the lungs. The pair of testicles have the shape of a bean and are cream colored. The results of research on the histological structure of bali duck testes consist of seminiferous tubules, interstitial tissue, leydig cells, and tunica albuginea. The results of histomorphometry measurements of bali duck testes showed that the wall thickness of the seminiferous tubules was 23.6760 ± 1.4425 (µm), the number of seminiferous tubules was 421.1000 ± 56.1396 and the thickness of the tunica albuginea was 29.5000 ± 5.048 (µm). Considering the importance of knowledge about the structure of bali duck testicles, it is recommended for future researchers to examine the structure and histomorphometry of bali duck testicles at different ages in the growing phase.
{"title":"A STRUCTURE AND HISTOMORPHOMETRY OF BALI DUCK TESTES IN THE GROWER PHASE","authors":"Ni Made Santi Rahayu Adiari, N. L. E. Setiasih, I. P. Sampurna, N. K. Suwiti, N. Susari, I. K. Suatha","doi":"10.24843/bulvet.2024.v16.i02.p17","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p17","url":null,"abstract":"Testes are male reproductive organs which are responsible for producing spermatozoa and steroid hormones. This study aims to determine the histology and histomorphometry structure of bali duck testes in the grower phase at the age of 12 weeks in one of the farms in Mengwi District, Badung Regency. The number of samples used in this research was 10 male bali ducks that were in good health. There are two examination methods carried out, namely the qualitative descriptive anatomical structure and histology examination method and the quantitative histomorphometry examination method of bali duck testicles which are analyzed using the One-Sample T-test with a confidence level of 95%. The results of this study show the anatomical structure of the bali duck testicles which are located in the abdominal cavity, between the kidneys and the lungs. The pair of testicles have the shape of a bean and are cream colored. The results of research on the histological structure of bali duck testes consist of seminiferous tubules, interstitial tissue, leydig cells, and tunica albuginea. The results of histomorphometry measurements of bali duck testes showed that the wall thickness of the seminiferous tubules was 23.6760 ± 1.4425 (µm), the number of seminiferous tubules was 421.1000 ± 56.1396 and the thickness of the tunica albuginea was 29.5000 ± 5.048 (µm). Considering the importance of knowledge about the structure of bali duck testicles, it is recommended for future researchers to examine the structure and histomorphometry of bali duck testicles at different ages in the growing phase.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"61 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140739277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p12
I. G. A. P. Pratama, N. L. E. Setiasih, Sri Kayati Widyastuti, N. K. Suwiti, N. Susari, I. K. Suatha
The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry
胰腺是消化系统的一部分,起着消化辅助器官的作用。胰腺有两种功能,即外分泌和内分泌。本研究旨在确定不同性别的巴厘鸭(Anas Sp)在生长阶段的胰腺结构和形态。本研究使用了 18 只巴厘岛鸭,将其分为两组,即雌雄各 9 只(3 月龄)。解剖结构的检查方法是使用双目光学显微镜直接观察和组织学结构。形态测量使用卡尺测量长度和宽度,天平测量胰腺重量,量杯测量体积。组织形态测量使用奥林巴斯细胞传感器标准应用软件进行测量。解剖学和组织学结构数据的结果采用定性描述分析法进行分析,而形态计量数据则采用独立样本 T 检验法进行分析。巴厘岛鸭胰腺的解剖结构扁平,像一个分叶的舌头,呈粉红色,由囊、朗格汉斯小体、盲肠、闰管、小叶内管、小叶间管、小叶内隔、小叶间隔、静脉和动脉组成。巴厘岛鸭胰腺的形态测量结果表明,雌雄鸭胰腺的形态差异显著(P0.05)。雌雄巴厘岛鸭胰腺的解剖结构和组织学相同,但在形态测量方面存在差异
{"title":"STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE","authors":"I. G. A. P. Pratama, N. L. E. Setiasih, Sri Kayati Widyastuti, N. K. Suwiti, N. Susari, I. K. Suatha","doi":"10.24843/bulvet.2024.v16.i02.p12","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p12","url":null,"abstract":"The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"75 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p15
Citra Widiawati, Ida Bagus Kade Suardana, G. A. Y. Kencana, N. L. E. Setiasih, T. S. Nindhia, A. A. S. Kendran
Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.
{"title":"POTENTIAL OF MENIRAN LEAF EXTRACT ON NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS","authors":"Citra Widiawati, Ida Bagus Kade Suardana, G. A. Y. Kencana, N. L. E. Setiasih, T. S. Nindhia, A. A. S. Kendran","doi":"10.24843/bulvet.2024.v16.i02.p15","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p15","url":null,"abstract":"Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"21 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In tropical climates such as Indonesia, the presence of ectoparasites occurs almost annually, which becomes one of the major problems due to ectoparasite infestations on ruminant farms. One problem occurred is the Boophilus sp. This study aimed to detect and identify causative factor in infestation of Boophilus sp. and correlation between tick infestation and incidence of blood parasite in Barru Regency. The study unit was the Bali cattle breeders spreaded in Mallusetasi District, Barru Regency. This study used a cross-sectional study to identify and determine the relationship between the causative factors of maintenance management and stock farmer knowledge level on the application of maintenance management in Bali cattle farms. The tabulated data of the maintenance management in Bali cattle breeding and breeder knowledge level causative factors against the tick ectoparasite infestation were descriptively analyzed and tested with a chi-square (χ2) method to measure the relationship of these factors and Boophilus sp. tick infestation at 95% of confidence level. The magnitude of relationship strength was calculated by the odd ratio (OR) test at a 95% of confidence level. Boophilus sp. tick ectoparasite infestation occurred in Bali cattle farms in Mallusetasi District, Barru Regency with an incidence rate of 68,8%. The causative factors that influence the incidence of tick infestations included the farmer educational level, breeder experience, number of maintenance, maintenance pattern, poor cage condition, and breeder's knowledge. The infestation of Boophilus sp. tick had a very significant correlation on the incidence of blood parasitic diseases. Therefore, to reduce tick infestation and the incidence of blood parasitic disease, planning of integrated control measures to create awareness about the importance and control of tick for livestock farmers is required.
{"title":"THE CAUSATIVE FACTOR DETECTION AND IDENTIFICATION OF BOOPHILUS SP. INFESTATION IN BALI CATTLE IN BARRU REGENCY, SOUTH SULAWESI","authors":"Suparmin Yuliani, Risha Catra Pradhany, Faizal Zakariya","doi":"10.24843/bulvet.2024.v16.i02.p16","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p16","url":null,"abstract":"In tropical climates such as Indonesia, the presence of ectoparasites occurs almost annually, which becomes one of the major problems due to ectoparasite infestations on ruminant farms. One problem occurred is the Boophilus sp. This study aimed to detect and identify causative factor in infestation of Boophilus sp. and correlation between tick infestation and incidence of blood parasite in Barru Regency. The study unit was the Bali cattle breeders spreaded in Mallusetasi District, Barru Regency. This study used a cross-sectional study to identify and determine the relationship between the causative factors of maintenance management and stock farmer knowledge level on the application of maintenance management in Bali cattle farms. The tabulated data of the maintenance management in Bali cattle breeding and breeder knowledge level causative factors against the tick ectoparasite infestation were descriptively analyzed and tested with a chi-square (χ2) method to measure the relationship of these factors and Boophilus sp. tick infestation at 95% of confidence level. The magnitude of relationship strength was calculated by the odd ratio (OR) test at a 95% of confidence level. Boophilus sp. tick ectoparasite infestation occurred in Bali cattle farms in Mallusetasi District, Barru Regency with an incidence rate of 68,8%. The causative factors that influence the incidence of tick infestations included the farmer educational level, breeder experience, number of maintenance, maintenance pattern, poor cage condition, and breeder's knowledge. The infestation of Boophilus sp. tick had a very significant correlation on the incidence of blood parasitic diseases. Therefore, to reduce tick infestation and the incidence of blood parasitic disease, planning of integrated control measures to create awareness about the importance and control of tick for livestock farmers is required.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"93 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.24843/bulvet.2024.v16.i02.p11
I. K. A. S. Prayoga, N. L. E. Setiasih, L. Heryani, N. K. Suwiti, N. Susari, I. K. Suatha
Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
浅胸肌是位于体表的胸肌,起着翅膀运动的作用。胫骨头盖肌是小腿肌肉结构中的顶部肌肉,具有支撑鸟类身体的功能。本研究旨在测定生长期雌雄巴厘岛鸭胸肌表层和胫骨颅骨肌的组织形态学。本研究使用了 20 只 12 周龄的巴厘岛鸭。用双目光学显微镜直接进行解剖检查和组织学结构检查。使用奥林巴斯 Cellsens Standard 应用程序测量组织形态学。解剖学和组织学结果采用描述性定性分析,组织形态测量采用方差分析检验,并估计平均值。胸肌浅层和胫骨颅骨肌的组织学结构由肌纤维、筋膜束、内膜、周膜和外膜结缔组织组成。不同性别的筋膜大小、韧带周围结缔组织厚度和胸浅肌内膜的组织形态学差异显著(P0.05)。由此可以得出结论,处于生长期的男性和女性的胸浅肌和胫骨颅骨肌在解剖结构上是相同的,但组织学结构的大小不同。 雄性和雌性巴厘鸭胸肌浅层的组织形态有显著差异(P<0.05),但胫骨颅骨肌没有显著差异。关于巴厘岛鸭在其他年龄段的肌肉,还需要进一步研究。
{"title":"HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE","authors":"I. K. A. S. Prayoga, N. L. E. Setiasih, L. Heryani, N. K. Suwiti, N. Susari, I. K. Suatha","doi":"10.24843/bulvet.2024.v16.i02.p11","DOIUrl":"https://doi.org/10.24843/bulvet.2024.v16.i02.p11","url":null,"abstract":"Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.","PeriodicalId":30995,"journal":{"name":"Buletin Veteriner Udayana","volume":"72 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}