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Antibacterial activities of moringa olifiera freeze dried extract on staphylococcus aureus 辣木冻干提取物对金黄色葡萄球菌的抑菌活性
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I2.1043
Wulan Angestia, Valendriyani Ningrum, Tai-Lin Lee, Shih-Chieh Lee, A. Bakar
Objective: The aim of this study is to determine the antibacterial activities of M. oleifera freeze dried extract on Staphylococcus aureus. Material and Methods: The antibacterial activities using two methods, microdilution method and agar diffusion method. The minimum inhibitory concentration is determined. The measurement of absorbance in microdilution method using a spectrophotometer at length wave 590 nm. The inhibition zone is measured using scalimeter. Results: The results showed both microdilution method and agar diffusion method present the inhibition activity of M. oleifera freeze dried extract on staphylococcus aureus. Conclusion: The finding confirmed M. oleifera freeze dried extract as the promising antibacterial natural agent, particularly on inhibiting S.
目的:研究油桐冻干提取物对金黄色葡萄球菌的抑菌活性。材料与方法:采用微量稀释法和琼脂扩散法两种方法进行抑菌。确定了最小抑菌浓度。用波长为590nm的分光光度计测定微量稀释法的吸光度。用比重计测定缓蚀带。结果:微稀释法和琼脂扩散法均表现出油桐冻干提取物对金黄色葡萄球菌的抑制作用。结论:油橄榄冻干提取物是一种很有前景的抗菌天然药物,对S。
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引用次数: 0
Cow gore related orofacial injuries: a review of cases managed at a semi-urban Hospital in Nigeria 牛粪相关的口面部损伤:对尼日利亚一家半城市医院处理的病例的审查
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.1118
B. Bamgbose, T. Owobu, B. Ojukwu, Auwal S. Balarabe
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引用次数: 0
Inferior alveolar nerve preservation for hemimandibulectomy and bridging plate reconstruction in monostotic fibrous dysplasia case 下牙槽神经保留在半下颌管切除术和桥接钢板重建中的应用
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V0I0.924
Agus Widodo, M. M. Rahmat, Prihartiningsih Prihartiningsih, C. Hasan
Objective: This paper aimed to report the inferior alveolar nerve preservation in cases of monostotic fibrous dysplasia performed by
目的:报道下牙槽神经保存术在单纯性纤维发育不良患者中的应用
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引用次数: 1
The effectiveness of oral health education and toothbrush training in decreasing plaque index score among Elementary Students in Jakarta 口腔健康教育和牙刷培训对降低雅加达小学生牙菌斑指数得分的效果
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.1102
Helwiah Umniyati, Benanziski Miegasivia, M. Zakki
Objective: To know the effectiveness of dental health education on decreasing plaque index score and increasing salivary pH among elementary school children in SD 01 Pagi Kampung Rawa, Central
目的:了解口腔健康教育对降低牙菌斑指数评分和提高小学生唾液pH值的效果
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引用次数: 0
A comparative study of platelet rich fibrin and bone graft in intrabony defect treatment: A systematic review 富血小板纤维蛋白与骨移植治疗骨内缺损的比较研究:系统综述
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V0I0.747
A. I. Djais, Heri Siswanto
Objective: To compare the result of treatment using platelet rich fibrin and bone graft in intrabony defect cases. Methods: Pubmed and Wiley online searches were conducted to identify articles published in dental journals from January 2013 to December 2017 focusing on platelet rich fibrin and bone graft in intrabony defect treatment. Manual searches of published full-text articles and related reviews were performed afterwards. There are 135 studies found, but only 4 studies met the inclusion criteria. Results: The initial database search produced 135 articles. All articles were selected for full-text review. A total 4 studies were selected for inclusion, with 107 patients as subjects. All of the studies showed the decreased of probing depth (PD), clinical attachment loss (CAL), intrabony defect (IBD) or radiograph defect depth (RDD) after 9 months follow up. The differences between baseline and clinical outcome measurement showed that single treatment using bone graft
目的:比较富血小板纤维蛋白与骨移植治疗骨内缺损的效果。方法:对2013年1月至2017年12月发表在牙科期刊上的关于富血小板纤维蛋白和骨移植治疗骨内缺损的文章进行Pubmed和Wiley在线检索。随后对已发表的全文文章和相关综述进行人工检索。共发现135项研究,但只有4项研究符合纳入标准。结果:最初的数据库搜索产生了135篇文章。选择所有文章进行全文综述。本研究共纳入4项研究,共纳入107例患者。所有研究均显示,随访9个月后,探探深度(PD)、临床附着丧失(CAL)、骨内缺损(IBD)或x线片缺损深度(RDD)均有所下降。基线和临床结果测量的差异表明,单一治疗采用骨移植
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引用次数: 3
The effectiveness of the use of plastic wrapping on dental unit work desks on the number of bacterial colonies in Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of North Sumatra (USU) 北苏门答腊大学牙科学院口腔颌面外科学系牙科单位办公桌使用塑料包装对细菌菌落数量的影响
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.828
O. A. Hanafiah, I. B. Siregar, Abdullah Oes, Elsa M. Sirait
Objective: The purpose of this research was to determine the effectiveness of the use of plastic wrapping on dental unit work desks on the number of bacterial colonies in Department of Oral and Maxillofacial Surgery Faculty of Dentistry USU. Material and Methods: In this study, sampling was carried out on tooth extraction patients in the Oral and Maxillofacial Surgery Department by using 2 dental chair units where one of the dental unit work desks used plastic wrap and the other did not. Then examination of the number of bacterial colonies was carried out in Microbiology Laboratory, Faculty of Mathematics and Natural Science USU. Results: Samples taken as many as 50 samples (25 pre-test samples and 25 posttest samples) in 5 different days showed a significant difference in the number of bacterial colonies (p<0.05). Conclusion: This study showed that there was a significant decrease in the number of bacterial colonies in the dental unit work desks that used plastic wrap compared to those that do not.
目的:本研究的目的是确定塑料包装在牙科单位工作台上使用对细菌菌落数量的影响。材料与方法:本研究采用2个牙椅单元对口腔颌面外科拔牙患者进行取样,其中一个牙椅单元的工作台使用保鲜膜,另一个未使用保鲜膜。然后在USU数学与自然科学学院微生物实验室对细菌菌落数量进行了检测。结果:5天内采集的样品多达50个(前测样品25个,后测样品25个),菌落数量差异有统计学意义(p<0.05)。结论:这项研究表明,与没有使用保鲜膜的办公桌相比,使用保鲜膜的牙科单位办公桌上的细菌菌落数量显著减少。
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引用次数: 0
Discoloration difference between acrylic artificial teeth exposed to filter- and electronic-cigarette smoke in the Laboratory Faculty of Dentistry, Muslim University of Indonesia in 2017 2017年印度尼西亚穆斯林大学牙科学院实验室中接触过滤烟和电子烟烟雾的丙烯酸人工牙的变色差异
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.821
C. Chotimah, M. Ramadan
Objective: To determine of artificial teeth color discoloration different between exposure of cigarette’s and electric cigarette’s smoke in the Laboratorium FKG UMI 2017. The simulator of smoking, artificial teeth, cigarette’s, CIE Lab. Material and Methods: Acrylic is most commonly used material for articial teeth. Acrylic is easily stained by food, beverage, chemical, and smoking habit. Cigarette and electric cigarette are commonly consumed by most people. Cigarette contains brownish substance called tar meanwhile electric cigarette contains of many chemical substances. Results: Data were recorded after exposure of cirarettes’s and analyzed by statistic test used Independent sampe T test whit the significance level α = 0.05. Cigarette smoke affects the color of acrylic artificial teeth. Meanwhile, electric cigarette effect the color of acrylic teeth but insignificantly. There are artifisial teeth color discoloration different between exposure of cigarette’s and electric cigarette’s smoke with
目的:研究FKG UMI 2017实验室中卷烟和电子烟烟雾暴露对人工牙变色的影响。吸烟模拟器,假牙,香烟,CIE实验室。材料和方法:丙烯酸是最常用的人工牙材料。亚克力很容易被食品、饮料、化学品和吸烟习惯所污染。香烟和电子烟是大多数人经常消费的。香烟含有一种叫焦油的棕色物质,而电子香烟含有多种化学物质。结果:对暴露后的数据进行记录,采用独立样本T检验进行统计检验,显著性水平α = 0.05。香烟的烟雾会影响丙烯酸人工牙的颜色。同时,电子烟对牙体颜色的影响不显著。香烟和电子烟对人工牙有不同的颜色变化
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引用次数: 1
Phyto-response effects of moringa oleifera leaves (moringa oleifera) ethanol extract on osteoblast and mandibular bone osteoclasts (rattus norvegicus) (experimental study using histopathology fluorescent light) 辣木叶(辣木叶)乙醇提取物对褐家鼠成骨细胞和下颌破骨细胞的植物响应效应(组织病理学荧光实验研究)
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.1065
Chairunas Chairunas, Basri A. Gani, M. Abdat, Salwa D. Kariza
Objective: To determine the phyto-response effect of the Kelor leaves ethanol extract (Moringa oleifera) in osteoblasts and osteoclasts cells of rat mandibular bones (Rattus norvegicus) in the atmosphere of fluorescent light histopathologically. Material and Methods: Manufacture of Kelor leaf ethanol extract by maceration method and ethanol 96%. The 10-tailed mice are divided into 1 mouse-tails that are only exposed to light, 3-tailed mice positive groups given the drug vitamin D, 3-tails of negative group rats given aquades, and 3-tails of treatment given the leaf ethanol extract. The treatment of rats was carried out on the 0 day, 7th Day, 14th and 21st days, then dieutanasia using ether anesthesia and carried out the cutting of the mandibular bone using bone, fixation, decalcification to the calculation of cells osteoblasts and osteoclasts with a microscope (Olympus BX-51) 400X magnification. Results: There was a significant difference between the positive control group, the negatives and the treatment group that applied the Kelor leaf ethanol extract to the rat mandibular bone and exposed to light for 24 hours. The treatment group applied the Kelor leaf ethanol extract has the highest number of osteoblasts cells on day 28. Conclusion: The Kelor leaf ethanol extract is effective in stimulating the osteoblasts cells of the bones that have been exposed to light in the long term. The Kelor leaf ethanol extract can balance the bone growth cycle significantly over vitamin D.
目的:从组织病理学角度探讨辣木叶乙醇提取物对褐家鼠下颌骨成骨细胞和破骨细胞在荧光环境下的植物响应作用。原料与方法:用浸渍法和96%乙醇制备克罗勒叶乙醇提取物。10尾小鼠分为单尾光照组、3尾阳性组给予药物维生素D、3尾阴性组给予药物aquades、3尾治疗组给予叶片乙醇提取物。大鼠治疗于第0天、第7天、第14天、第21天进行乙醚麻醉下安乐死,并在显微镜(Olympus BX-51) 400倍放大下行下颌骨切骨、固定、脱钙,计算细胞成骨细胞和破骨细胞。结果:阳性对照组、阴性组与克罗勒叶乙醇提取物作用于大鼠下颌骨并光照24h的处理组之间存在显著性差异。应用克罗勒叶乙醇提取物的处理组在第28天成骨细胞数量最多。结论:克罗勒叶乙醇提取物对长期暴露在光照下的骨的成骨细胞有一定的刺激作用。克罗勒叶乙醇提取物比维生素D更能平衡骨骼生长周期。
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引用次数: 0
Non-invasive early diagnosis of oral squamous cell carcinoma using piezoelectric biosensor 压电生物传感器在口腔鳞状细胞癌无创早期诊断中的应用
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V0I0.942
I. Rieuwpassa, S. Sumintarti, Yu Ri Kim, Megatriani Matandung, Gabriel SA. Matongan
Objective: To develop a device capable of distinguishing Oral squamous cell from other types of lesion so early diagnosis can be made noninvasively. Methods:The piezoelectric buzzer was coated with IgA EBV enzyme conjugate and antigen substance from Bionevan. The sensor was designed label free so further substance won’t be needed. The body of the device was made from white acrylic with 4mm thickness. The body was divided into 3 compartments each with different purpose. The main component consists of reagent coated piezoelectric buzzer and sensor. Both sensors are attached to Arduino UNO, breadboard, LIPO UBEC battery and LCD display network. The arduino UNO will read and process the difference between initial frequency and vibration frequency after the antigen were bind by the reagent, and shows the level of IgA in the LCD display. Results: The device worked and manage to show the level of IgA and presence of EBV in saliva but still need further improvement to make it more specific and valid Conclusion: Piezoelectric biosensor have shown promise to make early noninvasive diagnosis for OSCC
目的:研制一种能够将口腔鳞状细胞与其他类型病变区分开来的仪器,使其能够进行无创的早期诊断。方法:用Bionevan的IgA EBV酶偶联物和抗原物质包被压电蜂鸣器。该传感器被设计为无标签,因此不需要进一步的物质。该装置的主体由白色丙烯酸制成,厚度为4mm。身体被分成3个隔间,每个隔间都有不同的用途。主要由涂覆试剂的压电蜂鸣器和传感器组成。两个传感器都连接到Arduino UNO,面包板,LIPO UBEC电池和LCD显示网络。arduino UNO会读取和处理抗原被试剂结合后的初始频率和振动频率的差值,并在LCD显示屏上显示IgA的水平。结果:该装置能够显示唾液中IgA水平和EBV的存在,但仍需进一步改进以提高其特异性和有效性。结论:压电生物传感器有望对OSCC进行早期无创诊断
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引用次数: 0
The increase of fibroblast cells number in rat (rattus norvegicus) gingival wound after the application of moringa (moringa oleifera lam) fruit oil 辣木果油对褐家鼠牙龈创面成纤维细胞数量的影响
Pub Date : 2020-12-01 DOI: 10.15562/JDMFS.V5I3.1121
Asmawati Asmawati, B. Thalib, Nurhayaty Natsir, A. Thalib, Rafikah Hasyim
Objective: To determine the increase of fibroblast cell number in the rat (Rattus norvegicus) gingival wound after the application of moringa (Moringa oleifera Lam) fruit oil. Material and Methods: This study was an experimental posttest control group design using 18 male Wistar rats (Rattus norvegicus) that was divided into three groups. The sample was previously incised with a length of 4 mm using scalpel 15. Group 1 was applied with moringa fruit oil, group 2 was given aspirin, and group 3 was given at 0.5% CMC. The application was carried out every 24 hours, then on the third and seventh day, the rats were euthanized for histological preparations and then observed under the electric microscope. The length of the lesion was measured on the first, third, and seventh days. The fibroblast was then observed by counting the number of fibroblast cells per field of view using an Olympus C-21 microscope with an Optilab Advances camera with a 40x, 100x magnification, and 400x. The data were processed and analyzed using the SPSS Version 21 program. This study used a t-test to see the differences between the treatment group and the control group, and the Least Significantly Difference (LSD) test was used to see the size of the difference. Results: This study showed a significant increase in the number of fibroblasts in rat gingival mucosa on the third and seventh days. Conclusion: Moringa (Moringa oleifera Lam) fruit oil affects increasing the number of fibroblasts on the rat gingival mucosa (Rattus norvegicus).
目的:探讨辣木果油对褐家鼠牙龈创面成纤维细胞数量的影响。材料与方法:选用18只雄性褐家鼠(Rattus norvegicus),分为3组,采用实验性后测对照组设计。样品先前使用15号手术刀切割长度为4毫米。组1给予辣木果油,组2给予阿司匹林,组3给予0.5% CMC。每24小时给药一次,第3天和第7天处死大鼠进行组织学准备,电镜下观察。在第1、3、7天测量病变的长度。然后使用奥林巴斯C-21显微镜和Optilab advance相机(放大率分别为40倍、100倍和400倍)计数每个视场内成纤维细胞的数量来观察成纤维细胞。使用SPSS Version 21程序对数据进行处理和分析。本研究使用t检验来观察治疗组和对照组之间的差异,并使用最小显著差异(LSD)检验来观察差异的大小。结果:本研究显示大鼠牙龈粘膜成纤维细胞数量在第3天和第7天显著增加。结论:辣木(Moringa oleifera Lam)果油对大鼠牙龈黏膜成纤维细胞数量有增加作用。
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引用次数: 1
期刊
Journal of Dentomaxillofacial Science
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