Pub Date : 2020-01-01DOI: 10.21272/eumj.2020;8(3):268-275
L. Strilchuk, O. Zimba, I. Zhakun
Serum bilirubin, the end product of heme metabolism, is a routine biochemical parameter. Bilirubin is not a liver function parameter exclusively: its concentration correlates with ischemic heart disease (IHD) risk, estimated glomerular filtration rate, retinopathy or neuropathy in diabetes mellitus, atherosclerosis etc. The aim of this paper was to estimate the clinical value of bilirubin analysis according to literature data and own clinical observations in patients with IHD and acute and chronic rheumatologic diseases. Materials and methods. We conducted a literature overview in Pubmed database and domestic sources and also analyzed the standard examinaions of 515 patients: 353 patients with coronary heart disease (acute forms, coronary bypass grafting – 98; acute myocardial infarction, pharmacotherapy – 75; unstable angina pectoris – 101; stable angina pectoris – 79) and 162 rheumatologic patients (haemorrhagic vasculitis – 71; rheumatic fever – 57; chronic rheumatic heart disease with valvular defects – 34). Control group consisted of 22 patients with gastroduodenal zone diseases without helicobacter (esophagitis, gastritis, peptic ulcer). Results and discussion. It was revealed that in case of diseases with oxidative stress in their pathogenesis (acute forms of coronary heart disease, haemorrhagic vasculitis, rheumatic fever) bilirubin level was lower than in case of non-oxidative disorders (non-infectious esophagitis, gastritis, ulcer). Increase of inflammation potency was accompanied by bilirubin decrease. Correlation analysis showed that both bilirubin increase and decrease were unfavourable. Conclusions. Bilirubin concentration correlated with parameters of cytolysis, intoxication, anemia, inflammation, carbohydrate and lipid metabolism, heart structure. Bilirubin decrease associated with the increase of stenosis of coronary arteries (left, left circumflex and anterior interventricular) in a logarithmic way. Hypobilirubinemia (< 9.6 mkmol/L) significantly more often accompanied diseases with oxidative stress in pathogenesis, acute forms and more active systemic inflammation. Keywords total bilirubin, hypobilirubinemia, coronary heart disease, haemorrhagic vasculitis, rheumatic fever, chronic rheumatic heart disease.
{"title":"DECREASE IN SERUM BILIRUBIN AS AN UNFAVORABLE MARKER OF CARDIOVASCULAR DISORDERS","authors":"L. Strilchuk, O. Zimba, I. Zhakun","doi":"10.21272/eumj.2020;8(3):268-275","DOIUrl":"https://doi.org/10.21272/eumj.2020;8(3):268-275","url":null,"abstract":"Serum bilirubin, the end product of heme metabolism, is a routine biochemical parameter. Bilirubin is not a liver function parameter exclusively: its concentration correlates with ischemic heart disease (IHD) risk, estimated glomerular filtration rate, retinopathy or neuropathy in diabetes mellitus, atherosclerosis etc.\u0000The aim of this paper was to estimate the clinical value of bilirubin analysis according to literature data and own clinical observations in patients with IHD and acute and chronic rheumatologic diseases.\u0000Materials and methods. We conducted a literature overview in Pubmed database and domestic sources and also analyzed the standard examinaions of 515 patients: 353 patients with coronary heart disease (acute forms, coronary bypass grafting – 98; acute myocardial infarction, pharmacotherapy – 75; unstable angina pectoris – 101; stable angina pectoris – 79) and 162 rheumatologic patients (haemorrhagic vasculitis – 71; rheumatic fever – 57; chronic rheumatic heart disease with valvular defects – 34). Control group consisted of 22 patients with gastroduodenal zone diseases without helicobacter (esophagitis, gastritis, peptic ulcer).\u0000\u0000Results and discussion. It was revealed that in case of diseases with oxidative stress in their pathogenesis (acute forms of coronary heart disease, haemorrhagic vasculitis, rheumatic fever) bilirubin level was lower than in case of non-oxidative disorders (non-infectious esophagitis, gastritis, ulcer). Increase of inflammation potency was accompanied by bilirubin decrease. Correlation analysis showed that both bilirubin increase and decrease were unfavourable.\u0000Conclusions. Bilirubin concentration correlated with parameters of cytolysis, intoxication, anemia, inflammation, carbohydrate and lipid metabolism, heart structure. Bilirubin decrease associated with the increase of stenosis of coronary arteries (left, left circumflex and anterior interventricular) in a logarithmic way. Hypobilirubinemia (< 9.6 mkmol/L) significantly more often accompanied diseases with oxidative stress in pathogenesis, acute forms and more active systemic inflammation.\u0000\u0000Keywords\u0000total bilirubin, hypobilirubinemia, coronary heart disease, haemorrhagic vasculitis, rheumatic fever, chronic rheumatic heart disease.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124543482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):306-315
V. Lychko
The article gathers and analyses modern data from foreign and regional authors regarding the latest achievements in cellular technologies that have prospects in rehabilitation of neurological patients. For the replacement of damaged brain tissue, technologies directed for differentiation of stem cells in neuronal and glial directions are extremely promising. Today, it is feasible to expect that in the near future this will enable the transplantation of cells to activate the processes of neuroplasticity in recovery period. The data of own researches, which examined changes in structural and functional characteristics of brain tissue of rats with condition of experimental acute focal cerebral ischemia (AFCI) in dynamics of treatment cryopreserved cord blood serum (CCBS) was given for evaluation of its membrane protection, immune modulation and proangiogenic activity. The study was conducted on 60 outbred white male Wistar rats weighing 200±20 g. All animals were divided into 3 groups: 1st group (controls) – intact rats without trauma and treatment; 2nd group – animals after modelling AFCI without treatment; 3rd group – rats after modelling AFCI, which was administered CCBS. The results of the study indicated the stimulating effect of the components of CCBS on restoration of ultrastructure of the damaged capillaries, increasing their density, as well as the formation of new capillaries. It was found that the average area of the perivascular spaces, which is an indicator of vasogenic edema in rats of group 2 is 45 times higher than that in group 1, while in rats in group 3 treated with CCBS, this figure was exceeded 37 times. The average area of pericellular spaces, indicating the degree of cytotoxic edema, in rats of group 2 on the 7th day after AFCI is almost 23 times higher than the results of group 1. This indicator in rats of group 3 was increased by 20 times compared with group 2. On the 7th day of the experiment in rats of group 2, the surface area of endothelial cells was significantly larger than in rats of groups 1 and 3 by 54.1% and 31.6% respectively. Neurotrophic therapy is a very promising area of regenerative medicine, which requires further study in the use of growth factors.
{"title":"THE PERSPECTIVES OF CELLULAR THERAPY IN REHABILITATION TREATMENT OF NEUROLOGICAL PATIENTS (REVIEW OF LITERATURE WITH OWN RESEARCH DATA)","authors":"V. Lychko","doi":"10.21272/eumj.2019;7(4):306-315","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):306-315","url":null,"abstract":"The article gathers and analyses modern data from foreign and regional authors regarding the latest achievements in cellular technologies that have prospects in rehabilitation of neurological patients. For the replacement of damaged brain tissue, technologies directed for differentiation of stem cells in neuronal and glial directions are extremely promising. Today, it is feasible to expect that in the near future this will enable the transplantation of cells to activate the processes of neuroplasticity in recovery period.\u0000The data of own researches, which examined changes in structural and functional characteristics of brain tissue of rats with condition of experimental acute focal cerebral ischemia (AFCI) in dynamics of treatment cryopreserved cord blood serum (CCBS) was given for evaluation of its membrane protection, immune modulation and proangiogenic activity.\u0000The study was conducted on 60 outbred white male Wistar rats weighing 200±20 g. All animals were divided into 3 groups: 1st group (controls) – intact rats without trauma and treatment; 2nd group – animals after modelling AFCI without treatment; 3rd group – rats after modelling AFCI, which was administered CCBS.\u0000The results of the study indicated the stimulating effect of the components of CCBS on restoration of ultrastructure of the damaged capillaries, increasing their density, as well as the formation of new capillaries. It was found that the average area of the perivascular spaces, which is an indicator of vasogenic edema in rats of group 2 is 45 times higher than that in group 1, while in rats in group 3 treated with CCBS, this figure was exceeded 37 times. The average area of pericellular spaces, indicating the degree of cytotoxic edema, in rats of group 2 on the 7th day after AFCI is almost 23 times higher than the results of group 1. This indicator in rats of group 3 was increased by 20 times compared with group 2. On the 7th day of the experiment in rats of group 2, the surface area of endothelial cells was significantly larger than in rats of groups 1 and 3 by 54.1% and 31.6% respectively.\u0000Neurotrophic therapy is a very promising area of regenerative medicine, which requires further study in the use of growth factors.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"64 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115579176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):351-357
Y. Shkatula, Y. Badion, M. Novikov, Y. Khyzhnia
The work of medical workers is associated with constant psycho-emotional stress, which is caused by close contact with human suffering, the need to make immediate decisions, uncomfortable conditions of the pre-hospital stage and cases of aggressive and violent actions by patients or third parties. Statistics show that 54 to 84.8 % of medical workers have become victims of verbal or physical aggression annually. In 2013-2017, 543 crimes against life and health of medical workers on duty were registered in Ukraine. The purpose of the research was to study the causes, nature and risk factors of violent actions against emergency medical personnel with finding the ways to normalize the situation. Material and methods. An anonymous non-personified survey was conducted among 127 workers of the Sumy Regional Centre for Emergency Medical Care and Disaster Medicine. A modified questionnaire “Violence and aggression in the Health Service” (B. Mullan, F. Badger, 2007) was used in the study. It has been established that 74.8 % of emergency medical care and disaster medicine personnel were victims of violence caused by patients, their relatives or friends. Most often, the reasons for aggressive behaviour of the patient or third parties were the time of waiting for a medical worker and the suspicion of incompetence. According to the results of the survey, 35.43 % of employees believe that it is possible to improve the situation by completing and forming ambulance teams of a mixed type. Almost a third of the surveyed medical workers (24.41 %) indicated the need to provide personal protective equipment, another 14.96 % of respondents wanted better legal support and assistance. The authors come to the conclusion that it is necessary to solve the problem of the safety of a medical worker during an emergency call at the state legislative level. Particular attention should be paid to the further improvement of legal assistance, as well as to the development of measures to prevent violence.
医务工作者的工作与持续的心理-情绪压力有关，这种压力是由于与人类痛苦的密切接触、需要立即作出决定、院前阶段的不舒服条件以及患者或第三方的攻击性和暴力行为的情况造成的。统计数据显示，每年有54%至84.8%的医务工作者成为言语或身体攻击的受害者。2013-2017年，乌克兰登记了543起危害值班医务人员生命和健康的犯罪。研究的目的是研究针对急救医务人员的暴力行为的原因、性质和风险因素，并寻找使情况正常化的方法。材料和方法。对苏梅地区急救医疗和灾害医学中心的127名工作人员进行了一项匿名非拟人化调查。在研究中使用了一份修改过的问卷“卫生服务中的暴力和侵略”(B. Mullan, F. Badger, 2007)。据确定，74.8%的紧急医疗和灾害医疗人员是患者、其亲属或朋友造成的暴力的受害者。大多数情况下，患者或第三方的攻击行为的原因是等待医务人员的时间和怀疑无能。根据调查结果，35.43%的员工认为可以通过完成和组建混合型救护车队伍来改善这种情况。接受调查的医务工作者中，近三分之一(24.41%)表示需要提供个人防护装备，另有14.96%的受访者希望得到更好的法律支持和援助。作者认为，有必要在州立法层面解决紧急呼叫期间医务人员的安全问题。应特别注意进一步改进法律援助，以及制定防止暴力的措施。
{"title":"EMERGENCY MEDICINE: PROBLEMS OF VIOLENCE AND SAFETY AT WORK","authors":"Y. Shkatula, Y. Badion, M. Novikov, Y. Khyzhnia","doi":"10.21272/eumj.2019;7(4):351-357","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):351-357","url":null,"abstract":"The work of medical workers is associated with constant psycho-emotional stress, which is caused by close contact with human suffering, the need to make immediate decisions, uncomfortable conditions of the pre-hospital stage and cases of aggressive and violent actions by patients or third parties.\u0000Statistics show that 54 to 84.8 % of medical workers have become victims of verbal or physical aggression annually. In 2013-2017, 543 crimes against life and health of medical workers on duty were registered in Ukraine.\u0000The purpose of the research was to study the causes, nature and risk factors of violent actions against emergency medical personnel with finding the ways to normalize the situation.\u0000Material and methods. An anonymous non-personified survey was conducted among 127 workers of the Sumy Regional Centre for Emergency Medical Care and Disaster Medicine. A modified questionnaire “Violence and aggression in the Health Service” (B. Mullan, F. Badger, 2007) was used in the study.\u0000It has been established that 74.8 % of emergency medical care and disaster medicine personnel were victims of violence caused by patients, their relatives or friends. Most often, the reasons for aggressive behaviour of the patient or third parties were the time of waiting for a medical worker and the suspicion of incompetence.\u0000According to the results of the survey, 35.43 % of employees believe that it is possible to improve the situation by completing and forming ambulance teams of a mixed type. Almost a third of the surveyed medical workers (24.41 %) indicated the need to provide personal protective equipment, another 14.96 % of respondents wanted better legal support and assistance.\u0000The authors come to the conclusion that it is necessary to solve the problem of the safety of a medical worker during an emergency call at the state legislative level. Particular attention should be paid to the further improvement of legal assistance, as well as to the development of measures to prevent violence.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124314900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):377-385
V. Kovalev, Y. Diachenko, V. Malyshev, S. Rjabceva, O. Kolomiets, M. Lyndin, R. Moskalenko, A. Dovbysh, A. Romaniuk
Breast cancer is one of the most common cancer diseases in the world among women. The reliability of histological verification of breast cancer depends on pathologist’s experience, knowledge, his willingness to self-improve and study specialized literature. Digital pathology is also widely used for educational purposes, in telepathology, teleconsultation and research projects. Recently developed Whole Slide Image (WSI) system opens great opportunities in the histopathological diagnosis quality improvement. Digital whole-slide images provide the effective use of morphometry and various imaging techniques to assist pathologists in quantitative and qualitative evaluation of histopathological preparations. The development of software for morphological diagnosis is important for improving the quality of histological verification of diagnosis in oncopathology. The purpose of this work is to find and benchmark existing open-source software for the whole-slide histological images processing. Choosing an open source program is an important step in developing an automated breast cancer diagnosis program. The result is a detailed study of open-source software: ASAP, Orbit, Cytomine and QuPath. Their features and methods of image processing were analyzed. QuPath software has the best characteristics for extending it with an automated module for the cancer diagnosis. QuPath combines a user-friendly, easy-to-use interface, customizable functionality, and moderate computing power requirements. Besides, QuPath works with whole-slide images with immunohistochemical markers; features implemented in this software allow making a morphometric analysis. QuPath saves time for a graphical user interface development and provides a scalable system to add new key features. QuPath supports third-party MATLAB and Python extensions.
{"title":"COMPARATIVE FEATURES OF OPEN SOURCE SOFTWARE PRODUCTS FOR THE DEVELOPMENT OF AN AUTOMATED BREAST CANCER DIAGNOSTIC PROGRAM","authors":"V. Kovalev, Y. Diachenko, V. Malyshev, S. Rjabceva, O. Kolomiets, M. Lyndin, R. Moskalenko, A. Dovbysh, A. Romaniuk","doi":"10.21272/eumj.2019;7(4):377-385","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):377-385","url":null,"abstract":"Breast cancer is one of the most common cancer diseases in the world among women. The reliability of histological verification of breast cancer depends on pathologist’s experience, knowledge, his willingness to self-improve and study specialized literature. Digital pathology is also widely used for educational purposes, in telepathology, teleconsultation and research projects. Recently developed Whole Slide Image (WSI) system opens great opportunities in the histopathological diagnosis quality improvement. Digital whole-slide images provide the effective use of morphometry and various imaging techniques to assist pathologists in quantitative and qualitative evaluation of histopathological preparations. The development of software for morphological diagnosis is important for improving the quality of histological verification of diagnosis in oncopathology. The purpose of this work is to find and benchmark existing open-source software for the whole-slide histological images processing. Choosing an open source program is an important step in developing an automated breast cancer diagnosis program.\u0000The result is a detailed study of open-source software: ASAP, Orbit, Cytomine and QuPath. Their features and methods of image processing were analyzed. QuPath software has the best characteristics for extending it with an automated module for the cancer diagnosis. QuPath combines a user-friendly, easy-to-use interface, customizable functionality, and moderate computing power requirements. Besides, QuPath works with whole-slide images with immunohistochemical markers; features implemented in this software allow making a morphometric analysis.\u0000QuPath saves time for a graphical user interface development and provides a scalable system to add new key features. QuPath supports third-party MATLAB and Python extensions.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130402267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):336-340
P. Ishchenko, A. Borisenko
Introduction. Periodontal pathology accounts for many dental diseases, which encourages dentists and scientists to engage in this problem more actively. For the treatment of generalized periodontitis by the orthopedic component, we have proposed the claimed interdental splints, which were used in the orthopedic treatment of patients with this pathology in addition to the traditional ones. Defects in the lateral areas were recovered with the help of bridge structures. Purpose. To find out which of the splints used in the study are more physiological for the provoked parodontium in their area of responsibility using the bone area destruction factor. Materials and Methods. This clinical study was conducted in a group of 60 patients with generalized periodontitis in stabilization stage. Previously, they had undergone comprehensive treatment for generalized periodontitis. 30 subjects were included into the study group with periodontal condition in stabilization stage – the group with the declared tested splints. And the group with known traditional splints included 30 subjects. Results. The study found that after 18 months, the bone area destruction indicator decreased in patients with dentition defects where splints and bridges were used in the control group as compared to the value 6 months after treatment, by an average of 7.0 ± 1,1%, and it was only 2.9 ± 0.1% (p < 0.001) in the study group. Conclusions. During the whole period of the study in the group with dentition defects, the dynamics of restraining the increase in bone destruction in the study group is more obvious as compared to the control group. This indicates the feasibility of applying the claimed structures in this group of patients. The improvement in the percentage ratio was 4.1 ± 1.1% in 18 months.
{"title":"ANALYSIS OF THE USE OF CLAIMED AND TRADITIONAL SPLINTING ORTHOPEDIC STRUCTURES IN PATIENTS WITH GENERALIZED PERIODONTITIS DURING STABILIZATION STAGE WHO HAVE DENTITION DEFECT IN TERMS OF BONE AREA DESTRUCTION FACTOR","authors":"P. Ishchenko, A. Borisenko","doi":"10.21272/eumj.2019;7(4):336-340","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):336-340","url":null,"abstract":"Introduction. Periodontal pathology accounts for many dental diseases, which encourages dentists and scientists to engage in this problem more actively. For the treatment of generalized periodontitis by the orthopedic component, we have proposed the claimed interdental splints, which were used in the orthopedic treatment of patients with this pathology in addition to the traditional ones. Defects in the lateral areas were recovered with the help of bridge structures.\u0000 Purpose. To find out which of the splints used in the study are more physiological for the provoked parodontium in their area of responsibility using the bone area destruction factor.\u0000Materials and Methods. This clinical study was conducted in a group of 60 patients with generalized periodontitis in stabilization stage. Previously, they had undergone comprehensive treatment for generalized periodontitis. 30 subjects were included into the study group with periodontal condition in stabilization stage – the group with the declared tested splints. And the group with known traditional splints included 30 subjects.\u0000 Results. The study found that after 18 months, the bone area destruction indicator decreased in patients with dentition defects where splints and bridges were used in the control group as compared to the value 6 months after treatment, by an average of 7.0 ± 1,1%, and it was only 2.9 ± 0.1% (p < 0.001) in the study group.\u0000 Conclusions. During the whole period of the study in the group with dentition defects, the dynamics of restraining the increase in bone destruction in the study group is more obvious as compared to the control group. This indicates the feasibility of applying the claimed structures in this group of patients. The improvement in the percentage ratio was 4.1 ± 1.1% in 18 months.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121598770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):341-350
A. Loboda, O. Smiyan, S. V. Popov, Viktoriia Petrashenko, D. Loboda
Introduction. The study of the concentration of main electrolytes in serum of blood and erythrocytes in neonates with impaired renal function due to asphyxia is important, because it allows determining violations of their content and balance, tactics of infusion and diuretic therapy. The purpose of the work is explore the features of the content and balance of electrolytes (sodium, potassium, calcium, magnesium) in serum and red blood cells of newborns with disturbance kidney function due to asphyxia. Materials and methods. The study involved 200 term infants with signs of disturbance kidney function: 100 children who have suffered severe asphyxia, 100 children – with moderate asphyxia. Comparison group consisted of 20 infants without asphyxia at birth. The content of electrolytes determined by emission photometry, also expected ratios in pairs Na/K and Ca/Mg and transmembrane ratio of trace elements. Results and discussion. The critical period of formation electrolyte imbalances in neonates with impaired renal function due to moderate asphyxia is the early neonatal period, in case of severe asphyxia – all neonatal period. The feature of ischemic renal impairment in newborns is the development of serum hypernatremia and hyperkalemia, hypocalcemia and hypomagnesemia, decrease the ratio of Na/K and increase Ca/Mg. Red blood cell pool of macroelements in case of neonatorum ischemic nephropathy is characterized by the growth of sodium level and deficiency of potassium, calcium and magnesium, as well as growth transmineralisation Na/K ratio and decrease Ca/Mg. Growth transmembrane ratios relative to sodium and magnesium reflects their transport into the cell, and reducing ratios relative potassium and calcium indicates the predominance of these electrolyte transport in the extracellular fluid. Changes in serum and intracellular electrolyte content and balance must be considered during infusion therapy in infants with impaired renal function due to asphyxia.
{"title":"PECULIARITIES OF ELECTROLYTIC BALANCE IN THE BLOOD OF NEWBORNS WITH KIDNEY DAMAGE DUE TO ASPHYXIA","authors":"A. Loboda, O. Smiyan, S. V. Popov, Viktoriia Petrashenko, D. Loboda","doi":"10.21272/eumj.2019;7(4):341-350","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):341-350","url":null,"abstract":"Introduction. The study of the concentration of main electrolytes in serum of blood and erythrocytes in neonates with impaired renal function due to asphyxia is important, because it allows determining violations of their content and balance, tactics of infusion and diuretic therapy. The purpose of the work is explore the features of the content and balance of electrolytes (sodium, potassium, calcium, magnesium) in serum and red blood cells of newborns with disturbance kidney function due to asphyxia.\u0000Materials and methods. The study involved 200 term infants with signs of disturbance kidney function: 100 children who have suffered severe asphyxia, 100 children – with moderate asphyxia. Comparison group consisted of 20 infants without asphyxia at birth. The content of electrolytes determined by emission photometry, also expected ratios in pairs Na/K and Ca/Mg and transmembrane ratio of trace elements.\u0000Results and discussion. The critical period of formation electrolyte imbalances in neonates with impaired renal function due to moderate asphyxia is the early neonatal period, in case of severe asphyxia – all neonatal period. The feature of ischemic renal impairment in newborns is the development of serum hypernatremia and hyperkalemia, hypocalcemia and hypomagnesemia, decrease the ratio of Na/K and increase Ca/Mg. Red blood cell pool of macroelements in case of neonatorum ischemic nephropathy is characterized by the growth of sodium level and deficiency of potassium, calcium and magnesium, as well as growth transmineralisation Na/K ratio and decrease Ca/Mg. Growth transmembrane ratios relative to sodium and magnesium reflects their transport into the cell, and reducing ratios relative potassium and calcium indicates the predominance of these electrolyte transport in the extracellular fluid. Changes in serum and intracellular electrolyte content and balance must be considered during infusion therapy in infants with impaired renal function due to asphyxia.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133877222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):316-322
A. Antoniv
The aim of the research − to find out of changes fibrinolytic activity of blood in patients with non-alcoholic fatty liver on the background of obesity, depending on the presence of comorbid chronic kidney disease. Material and methods of research: 444 patients were examined: 84 of them were with NAFLD and class I obesity (group 1), which contained 2 subgroups: 32 patients with non-alcoholic steatosis (NAS) and 52 patients with non-alcoholic steatohepatitis (NASH); 270 patients with NAFLD with comorbid class I obesity and CKD І–ІІІ stage (group 2), including 110 patients with NAS and 160 patients with NASH. The control group consisted of 90 patients with CKD of І–ІІІ stage with normal body weight (group 3). To determine the dependence of the NAFLD course on the form and stage of the CKD, the group of patients was randomized according to age, sex, degree of obesity, and activity of NASH. Research results. The study of fibrinolytic activity of blood showed that total fibrinolytic activity (TFA) of blood plasma in patients of all groups was significantly lower than the control indexes: in patients with NAS – by 7.1%, patients with NAS with CKD – by 14.9%, patients with NASH – by 17.2%, patients with NASH with CKD – by 18.9%, patients with CKD – by 10.6% (p <0.05) with the presence of a probable intergroup difference between groups with comorbidity and isolated course of CKD (p <0.05). The suppression of TFA occurred through the decrease of EF: in patients with NAS the index is significantly lower than that in the controls by 1.2 times, in patients with NAS with CKD – by 1.4 times, in patients with NASH – by 1.7 times, in the group of patients with NASH and CKD – by 1.9 times, while in the group of patients with CKD, the suppression of EF was registered – 1.3 times (p <0.05). At the same time, the NEF in patients of all groups increased in comparison with the AHP group: in patients with NAS – by 1.2 times, in patients with NAS with CKD – by 1.3 times, in patients with NASH – by 1.4 times, in the group of patients with NASH with CKD – 1.5 times, while in the group of patients with CKD the activation of NEF was registered 1.2 times (p <0.05), with the presence of a probable difference between the groups with comorbidity and isolated course of CKD (p <0.05). Conclusion. Analysis of hemostasis and fibrinolysis indices in examined patients with NASH, depending on the stage of CKD showed that with the growth of the CKD stage, the activity of the cohort increases, with the exception of the fibrinogen content (most likely due to coagulopathy consumption), the activity of the anti-coagulants decreases, the total and enzymatic activity of fibrinolysis is reduced, and non-enzymatic compensator increases. Thus, metabolic intoxication, oxidative stress, which accompany the flow of NAFLD with obesity and CKD, promote the activation of the kallikrein-kinin system, the formation of plasma and thrombin, with subsequent disturbance of equilibrium between them, the dev
{"title":"ROLE OF FIBRINOLYTIC ACTIVITY OF BLOOD IN PATHOGENESIS OF NON-ALCOHOLIC FATTY LIVER DISEASE AND CHRONIC KIDNEY DISEASE (ORIGINAL RESEARCH)","authors":"A. Antoniv","doi":"10.21272/eumj.2019;7(4):316-322","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):316-322","url":null,"abstract":"The aim of the research − to find out of changes fibrinolytic activity of blood in patients with non-alcoholic fatty liver on the background of obesity, depending on the presence of comorbid chronic kidney disease.\u0000Material and methods of research: 444 patients were examined: 84 of them were with NAFLD and class I obesity (group 1), which contained 2 subgroups: 32 patients with non-alcoholic steatosis (NAS) and 52 patients with non-alcoholic steatohepatitis (NASH); 270 patients with NAFLD with comorbid class I obesity and CKD І–ІІІ stage (group 2), including 110 patients with NAS and 160 patients with NASH. The control group consisted of 90 patients with CKD of І–ІІІ stage with normal body weight (group 3). To determine the dependence of the NAFLD course on the form and stage of the CKD, the group of patients was randomized according to age, sex, degree of obesity, and activity of NASH.\u0000Research results. The study of fibrinolytic activity of blood showed that total fibrinolytic activity (TFA) of blood plasma in patients of all groups was significantly lower than the control indexes: in patients with NAS – by 7.1%, patients with NAS with CKD – by 14.9%, patients with NASH – by 17.2%, patients with NASH with CKD – by 18.9%, patients with CKD – by 10.6% (p <0.05) with the presence of a probable intergroup difference between groups with comorbidity and isolated course of CKD (p <0.05). The suppression of TFA occurred through the decrease of EF: in patients with NAS the index is significantly lower than that in the controls by 1.2 times, in patients with NAS with CKD – by 1.4 times, in patients with NASH – by 1.7 times, in the group of patients with NASH and CKD – by 1.9 times, while in the group of patients with CKD, the suppression of EF was registered – 1.3 times (p <0.05). At the same time, the NEF in patients of all groups increased in comparison with the AHP group: in patients with NAS – by 1.2 times, in patients with NAS with CKD – by 1.3 times, in patients with NASH – by 1.4 times, in the group of patients with NASH with CKD – 1.5 times, while in the group of patients with CKD the activation of NEF was registered 1.2 times (p <0.05), with the presence of a probable difference between the groups with comorbidity and isolated course of CKD (p <0.05).\u0000Conclusion. Analysis of hemostasis and fibrinolysis indices in examined patients with NASH, depending on the stage of CKD showed that with the growth of the CKD stage, the activity of the cohort increases, with the exception of the fibrinogen content (most likely due to coagulopathy consumption), the activity of the anti-coagulants decreases, the total and enzymatic activity of fibrinolysis is reduced, and non-enzymatic compensator increases. Thus, metabolic intoxication, oxidative stress, which accompany the flow of NAFLD with obesity and CKD, promote the activation of the kallikrein-kinin system, the formation of plasma and thrombin, with subsequent disturbance of equilibrium between them, the dev","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134506290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):358-364
N. Kyrychenko, N. Opolonska, O. Stepanets
Patients with type 2 diabetes mellitus (DM) may develop cardiomyopathy independently of such risk factors as arterial hypertension and coronary heart disease. Myocardial dysfunction in diabetes mellitus may vary from subclinical forms of left ventricular dysfunction to heart failure. It was suggested that diastolic left ventricular dysfunction is one of the earliest signs of myocardial injury in diabetes mellitus and plays a key role in the formation of diabetic cardiomyopathy. The aim of our study was to evaluate the effect of diabetes on the clinical and laboratory status of women with hypertension, obesity, and left ventricular diastolic dysfunction (LVDD). Materials and methods. We examined 80 patients aged 40 to 60 years with stage 2, grade II and grade III hypertension, class I–III obesity, grade 1 LVDD and preserved ejection fraction. Depending on the presence or absence of diabetes, the cohort of patients was divided into two groups: patients with diabetes were assigned to group 1 and non-diabetes patients – to group 2. Statistical processing was performed using Statistica for Windows version 6.0. Results. Patients had tendency to increased body mass index (BMI) in the DM group, but without significant differences. The results of the 6-minute walk test showed a tendency to decreased distance in the group of patients with diabetes. There was an increase in leptin levels and a decrease in adiponectin in patients with diabetes without significant differences. Levels of IL-6, glycosylated hemoglobin, and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index were significantly higher in the cohort of patients with diabetes (p <0.05). Conclusions. Women aged 40–60 years with LVDD with hypertension, class I–III obesity and type 2 diabetes mellitus differ from similar cohorts of patients without diabetes with a tendency to increased BMI levels, leptinemia, and decrease in distance of 6-minute walk test, LV ejection fraction and blood adiponectin level; they have significantly higher blood levels of interleukin-6, glycosylated hemoglobin (HbA1c) and HOMA-IR; indicators of diastolic function in the group of patients with diabetes tend to worsen the parameters of diastolic filling of the LV even in grade 1 DD.
2型糖尿病(DM)患者可能独立于动脉高血压和冠心病等危险因素发生心肌病。糖尿病的心肌功能障碍可能从亚临床形式的左心室功能障碍到心力衰竭不等。提示舒张性左心室功能障碍是糖尿病心肌损伤的早期征象之一，在糖尿病性心肌病的形成中起关键作用。本研究的目的是评估糖尿病对合并高血压、肥胖和左心室舒张功能不全(LVDD)的女性临床和实验室状态的影响。材料和方法。我们研究了80例年龄在40 - 60岁的2期、II级和III级高血压、I-III级肥胖、1级LVDD和保留射血分数的患者。根据是否患有糖尿病，患者被分为两组:糖尿病患者被分配到第一组，非糖尿病患者被分配到第二组。统计处理使用Statistica for Windows version 6.0.Results。DM组患者体质指数(BMI)有升高的趋势，但差异无统计学意义。6分钟步行测试的结果显示，糖尿病患者组的步行距离有减少的趋势。糖尿病患者瘦素水平升高，脂联素水平降低，但无显著差异。糖尿病患者IL-6水平、糖化血红蛋白水平及胰岛素抵抗稳态模型评估(HOMA-IR)指数均显著升高(p <0.05)。40-60岁LVDD合并高血压、I-III级肥胖和2型糖尿病的女性与同类无糖尿病患者不同，BMI水平升高、瘦素血症、6分钟步行距离试验、左室射血分数和血脂联素水平降低;他们的白细胞介素-6、糖化血红蛋白(HbA1c)和HOMA-IR水平明显较高;糖尿病患者组的舒张功能指标甚至在1级DD时有使左室舒张充盈参数恶化的趋势。
{"title":"EFFECTS OF TYPE 2 DIABETES MELLITUS ON CLINICAL AND LABORATORY STATUS OF WOMEN WITH ARTERIAL HYPERTENSION, OBESITY, AND LEFT VENTRICULAR DIASTOLIC DYSFUNCTION","authors":"N. Kyrychenko, N. Opolonska, O. Stepanets","doi":"10.21272/eumj.2019;7(4):358-364","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):358-364","url":null,"abstract":"Patients with type 2 diabetes mellitus (DM) may develop cardiomyopathy independently of such risk factors as arterial hypertension and coronary heart disease. Myocardial dysfunction in diabetes mellitus may vary from subclinical forms of left ventricular dysfunction to heart failure. It was suggested that diastolic left ventricular dysfunction is one of the earliest signs of myocardial injury in diabetes mellitus and plays a key role in the formation of diabetic cardiomyopathy.\u0000The aim of our study was to evaluate the effect of diabetes on the clinical and laboratory status of women with hypertension, obesity, and left ventricular diastolic dysfunction (LVDD).\u0000Materials and methods. We examined 80 patients aged 40 to 60 years with stage 2, grade II and grade III hypertension, class I–III obesity, grade 1 LVDD and preserved ejection fraction. Depending on the presence or absence of diabetes, the cohort of patients was divided into two groups: patients with diabetes were assigned to group 1 and non-diabetes patients – to group 2. Statistical processing was performed using Statistica for Windows version 6.0.\u0000Results. Patients had tendency to increased body mass index (BMI) in the DM group, but without significant differences. The results of the 6-minute walk test showed a tendency to decreased distance in the group of patients with diabetes. There was an increase in leptin levels and a decrease in adiponectin in patients with diabetes without significant differences. Levels of IL-6, glycosylated hemoglobin, and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index were significantly higher in the cohort of patients with diabetes (p <0.05).\u0000Conclusions. Women aged 40–60 years with LVDD with hypertension, class I–III obesity and type 2 diabetes mellitus differ from similar cohorts of patients without diabetes with a tendency to increased BMI levels, leptinemia, and decrease in distance of 6-minute walk test, LV ejection fraction and blood adiponectin level; they have significantly higher blood levels of interleukin-6, glycosylated hemoglobin (HbA1c) and HOMA-IR; indicators of diastolic function in the group of patients with diabetes tend to worsen the parameters of diastolic filling of the LV even in grade 1 DD.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126620950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):365-371
T. Filonova, V. Savvo, Y. Sorokolat
Cardiovascular pathology is one of the urgent problems of modern medicine, including children. The peculiarity of most cardiovascular diseases in children lies in their prolonged formation, tendency to chronic course, less apparent clinical manifestations in the first stages of the disease. Objective. In childhood, large-scale clinical epidemiological studies have not yet become widespread. But the available statistics allows analyzing trends and patterns that reflect the features of cardiovascular pathology in childhood, its role in health of the child population, which was the objective of this paper. Materials and methods. The analysis of medical and statistical documents of 1825 children aged 0–18 years followed up for cardiomyopathies of dysplastic genesis (DGC) in healthcare facilities of Kharkiv. The indications for following up were clinical symptoms and cardiac ultrasound findings during outpatient or inpatient examination in the absence of organic cardiovascular diseases. Results. Most often, clinical manifestation and, accordingly, diagnosis were observed from 4 to 11 years. Most minor cardiac abnormalities persist over the lifetime, and clinical significance may increase with age, contributing to the development of pathology or becoming a risk factor for cardiac pathology. Conclusions. The structure of minor cardiac abnormalities among the children of the dispensary group is heterogeneous: prolapse of the mitral valve (mostly isolated), abnormal chords of the left ventricle and open foramen ovale are most frequently recorded; prolapse of other valves and aneurysms of the atrial septum are very rare. Primary diagnosis of minor cardiac abnormalities mainly takes place at age 4 to 11 years. Summarizing the findings, it can be noted that minor cardiac abnormalities have a relatively high detection rate and constitute a large and heterogeneous dispensary monitoring group.
{"title":"MINOR CARDIAC ABNORMALITIES IN CHILDREN OF KHARKIV: PECULIARITIES OF STRUCTURE AND DISTRIBUTION","authors":"T. Filonova, V. Savvo, Y. Sorokolat","doi":"10.21272/eumj.2019;7(4):365-371","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):365-371","url":null,"abstract":"Cardiovascular pathology is one of the urgent problems of modern medicine, including children. The peculiarity of most cardiovascular diseases in children lies in their prolonged formation, tendency to chronic course, less apparent clinical manifestations in the first stages of the disease.\u0000Objective. In childhood, large-scale clinical epidemiological studies have not yet become widespread. But the available statistics allows analyzing trends and patterns that reflect the features of cardiovascular pathology in childhood, its role in health of the child population, which was the objective of this paper.\u0000Materials and methods. The analysis of medical and statistical documents of 1825 children aged 0–18 years followed up for cardiomyopathies of dysplastic genesis (DGC) in healthcare facilities of Kharkiv. The indications for following up were clinical symptoms and cardiac ultrasound findings during outpatient or inpatient examination in the absence of organic cardiovascular diseases.\u0000Results. Most often, clinical manifestation and, accordingly, diagnosis were observed from 4 to 11 years. Most minor cardiac abnormalities persist over the lifetime, and clinical significance may increase with age, contributing to the development of pathology or becoming a risk factor for cardiac pathology.\u0000Conclusions. The structure of minor cardiac abnormalities among the children of the dispensary group is heterogeneous: prolapse of the mitral valve (mostly isolated), abnormal chords of the left ventricle and open foramen ovale are most frequently recorded; prolapse of other valves and aneurysms of the atrial septum are very rare. Primary diagnosis of minor cardiac abnormalities mainly takes place at age 4 to 11 years. Summarizing the findings, it can be noted that minor cardiac abnormalities have a relatively high detection rate and constitute a large and heterogeneous dispensary monitoring group.","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"283 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121640874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-29DOI: 10.21272/eumj.2019;7(4):386-395
P. Myronov, V. Bugaiov, O. Tymakova, M. Pogorielov, A. Opanasyuk
The treatment of purulent wounds is an important problem of modern surgery. Antibiotic resistance of bacteria dramatically reduces the effectiveness of traditional methods of treatment. Previous studies have indicated that silver nanoparticles have good antibacterial activity and do not cause bacterial resistance. Low-frequency ultrasound improves the bactericidal properties of nanoparticles, as well as plays an important role in cleaning wounds from purulent-necrotic tissue and delivering nanoparticles to the site of infection. The combination of the properties of silver nanoparticles and low-frequency ultrasound requires careful investigation in the treatment of purulent wounds. The purpose of the study was to substantiate the effectiveness of the treatment of purulent wounds with silver nanoparticles and low-frequency ultrasound by cytological examination. Materials and Methods. This study was carried out on 60 laboratory rats, which were equally divided into 3 groups. In the first group, treatment was carried out by low-frequency ultrasound; in the second group, a solution of silver nanoparticles was used together with low-frequency ultrasound; in the third, control group, a 0.05% Chlorhexidine solution was used. Silver nanoparticles with a size of 10-60 nm were synthesized by the polyol method. Results. The study showed that there were no statistically significant differences between the ultrasound, silver nanoparticles/ultrasound and Chlorhexidine groups on the first day. On the third day, a statistically significant increase in phagocytic neutrophilic leukocytes and fibroblasts was observed in the silver nanoparticles/ultrasound group compared with the first day. In comparison with the Chlorhexidine group, the number of monocytes (2.2 and 4.2 times) and macrophages (1.4 and 1.9 times) increased in the ultrasound and silver nanoparticles/ultrasound groups respectively, and the necrotic type of cytograms was not determined. On the seventh day, granulation tissue began to appear in the silver nanoparticles/ultrasound group, the percentage of leukocyte destruction decreased (by 4.5 times), microorganisms were almost not detected, regenerative types of cytograms appeared for the first time. The number of neutrophils in the control group exceeded the analogous parameter of the ultrasound group by 2.4 times and the silver nanoparticles/ultrasound group by 3.8 times. The number of fibroblasts became significantly larger in the ultrasound (2.2 times) and the silver nanoparticles/ultrasound (2.3 times) groups, compared with the group where Chlorhexidine was used. On the tenth day, the number of fibroblasts and cells of the monocytic-macrophage series increased in the control group, which indicates later regenerative processes. On the tenth day, there was a complete epithelization of wounds in the silver nanoparticles/ultrasound group, while healing occurred on day 12 in the ultrasound group, and on day 21 in the control group. Conclusions. The combi
{"title":"CYTOLOGICAL EXAMINATION OF EXPERIMENTAL PURULENT WOUNDS IN THE TREATMENT OF SILVER NANOPARTICLES IN ULTRASOUND CAVITATION","authors":"P. Myronov, V. Bugaiov, O. Tymakova, M. Pogorielov, A. Opanasyuk","doi":"10.21272/eumj.2019;7(4):386-395","DOIUrl":"https://doi.org/10.21272/eumj.2019;7(4):386-395","url":null,"abstract":"The treatment of purulent wounds is an important problem of modern surgery. Antibiotic resistance of bacteria dramatically reduces the effectiveness of traditional methods of treatment. Previous studies have indicated that silver nanoparticles have good antibacterial activity and do not cause bacterial resistance. Low-frequency ultrasound improves the bactericidal properties of nanoparticles, as well as plays an important role in cleaning wounds from purulent-necrotic tissue and delivering nanoparticles to the site of infection. The combination of the properties of silver nanoparticles and low-frequency ultrasound requires careful investigation in the treatment of purulent wounds.\u0000The purpose of the study was to substantiate the effectiveness of the treatment of purulent wounds with silver nanoparticles and low-frequency ultrasound by cytological examination.\u0000Materials and Methods. This study was carried out on 60 laboratory rats, which were equally divided into 3 groups. In the first group, treatment was carried out by low-frequency ultrasound; in the second group, a solution of silver nanoparticles was used together with low-frequency ultrasound; in the third, control group, a 0.05% Chlorhexidine solution was used. Silver nanoparticles with a size of 10-60 nm were synthesized by the polyol method.\u0000Results. The study showed that there were no statistically significant differences between the ultrasound, silver nanoparticles/ultrasound and Chlorhexidine groups on the first day. On the third day, a statistically significant increase in phagocytic neutrophilic leukocytes and fibroblasts was observed in the silver nanoparticles/ultrasound group compared with the first day. In comparison with the Chlorhexidine group, the number of monocytes (2.2 and 4.2 times) and macrophages (1.4 and 1.9 times) increased in the ultrasound and silver nanoparticles/ultrasound groups respectively, and the necrotic type of cytograms was not determined. On the seventh day, granulation tissue began to appear in the silver nanoparticles/ultrasound group, the percentage of leukocyte destruction decreased (by 4.5 times), microorganisms were almost not detected, regenerative types of cytograms appeared for the first time. The number of neutrophils in the control group exceeded the analogous parameter of the ultrasound group by 2.4 times and the silver nanoparticles/ultrasound group by 3.8 times. The number of fibroblasts became significantly larger in the ultrasound (2.2 times) and the silver nanoparticles/ultrasound (2.3 times) groups, compared with the group where Chlorhexidine was used. On the tenth day, the number of fibroblasts and cells of the monocytic-macrophage series increased in the control group, which indicates later regenerative processes. On the tenth day, there was a complete epithelization of wounds in the silver nanoparticles/ultrasound group, while healing occurred on day 12 in the ultrasound group, and on day 21 in the control group.\u0000Conclusions. The combi","PeriodicalId":315243,"journal":{"name":"Eastern Ukrainian Medical Journal","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128036932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：