Johan Permada, Witjaksono Witjaksono, F. X. Wagiman
Khapra beetle, Trogoderma granarium Evert (Coleoptera: Dermestidae) is the most important stored pest and was first discovered in Jakarta grain storages in 1972. Since then, quarantine procedures have been massively carried out to prevent khapra beetles to spread in Indonesia. In 2009, Indonesia has been declared as free area from Khapra beetle. However, Indonesia still import grain from non-Khapra beetle free countries and supervision must be carried out continuously to prevent Khapra beetle reinfestation. This survey was conducted at warehouses in five areas of Jakarta, Tangerang, and Bekasi from October 2016 to February 2017 with high risk due to its role as one of the main entry ports and storage of imported grains. Observation was done using probe sampling, direct observation and traps with attractant. Results showed that several insects were found from imported seeds in the surveyed area, but no Khapra beetles were found. It showed that non-Khapra free countries have implemented the required Sanitary Phytosanitary. In addition, it implies that survey methods using attractant traps could complement the probe sampling methods and direct observation that are currently used.
{"title":"Monitoring of Khapra Beetle in Jakarta, Tangerang, and Bekasi as Part of Khapra Beetle Free Area Maintenance Program in Indonesia","authors":"Johan Permada, Witjaksono Witjaksono, F. X. Wagiman","doi":"10.22146/jpti.52892","DOIUrl":"https://doi.org/10.22146/jpti.52892","url":null,"abstract":"Khapra beetle, Trogoderma granarium Evert (Coleoptera: Dermestidae) is the most important stored pest and was first discovered in Jakarta grain storages in 1972. Since then, quarantine procedures have been massively carried out to prevent khapra beetles to spread in Indonesia. In 2009, Indonesia has been declared as free area from Khapra beetle. However, Indonesia still import grain from non-Khapra beetle free countries and supervision must be carried out continuously to prevent Khapra beetle reinfestation. This survey was conducted at warehouses in five areas of Jakarta, Tangerang, and Bekasi from October 2016 to February 2017 with high risk due to its role as one of the main entry ports and storage of imported grains. Observation was done using probe sampling, direct observation and traps with attractant. Results showed that several insects were found from imported seeds in the surveyed area, but no Khapra beetles were found. It showed that non-Khapra free countries have implemented the required Sanitary Phytosanitary. In addition, it implies that survey methods using attractant traps could complement the probe sampling methods and direct observation that are currently used.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45188064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Actinomycetes are Gram-positive filamentous bacteria used as biocontrol agents against pathogens. Currently, little research on actinomycetes has been published in Indonesia. The study provides issues related to the most relevant keywords in actinomycetes, the most productive authors and countries on actinomycetes research, current research on actinomycetes, and future topics in actinomycetes research. This paper aims to understand the current status and trend of research on actinomycetes in biological control, especially their acts as biocontrol agents. We used VOSviewer and CiteSpace software to perform a scientometric and bibliometric analysis. Out of 452 Scopus publications published between 1971 and 2022. Research on actinomycetes has increased rapidly since 2000, leading to an exponential trend. The result shows that >50% of papers focus on Agricultural and Biological Sciences and indicate that actinomycetes are mainly applied as biocontrol agents. China is the most documented and powerful country, followed by the United States and Iran. In contrast, Indonesia ranks 19th in the world with nine published papers. Scientometric analysis shows Shahidi Bonjar nominated as the strongest citation burst author (4.01). Their topic is mainly on the biological control of pathogens by Actinomycetes, especially Streptomyces sp., while fungi are a dominant pathogen. These findings are related to bibliometric analysis, showing Streptomyces spp. and fungi are two primary keywords in the Scopus database, found on 183 and 139 documents. We found actinomycete research focuses on their ability to manage plant diseases naturally. In the future, we predict actinobacterial research is still essential to biological control, particularly those involving Streptomyces species. Also, several important topics are associated with their activities in controlling pests (nematode), postharvest disease, and growth promoter ability.
{"title":"A Scientometric and Bibliometric Analysis for Actinomycetes Research - Current Status and Future Trends","authors":"Syahri Syahri, R. U. Somantri","doi":"10.22146/jpti.77558","DOIUrl":"https://doi.org/10.22146/jpti.77558","url":null,"abstract":"Actinomycetes are Gram-positive filamentous bacteria used as biocontrol agents against pathogens. Currently, little research on actinomycetes has been published in Indonesia. The study provides issues related to the most relevant keywords in actinomycetes, the most productive authors and countries on actinomycetes research, current research on actinomycetes, and future topics in actinomycetes research. This paper aims to understand the current status and trend of research on actinomycetes in biological control, especially their acts as biocontrol agents. We used VOSviewer and CiteSpace software to perform a scientometric and bibliometric analysis. Out of 452 Scopus publications published between 1971 and 2022. Research on actinomycetes has increased rapidly since 2000, leading to an exponential trend. The result shows that >50% of papers focus on Agricultural and Biological Sciences and indicate that actinomycetes are mainly applied as biocontrol agents. China is the most documented and powerful country, followed by the United States and Iran. In contrast, Indonesia ranks 19th in the world with nine published papers. Scientometric analysis shows Shahidi Bonjar nominated as the strongest citation burst author (4.01). Their topic is mainly on the biological control of pathogens by Actinomycetes, especially Streptomyces sp., while fungi are a dominant pathogen. These findings are related to bibliometric analysis, showing Streptomyces spp. and fungi are two primary keywords in the Scopus database, found on 183 and 139 documents. We found actinomycete research focuses on their ability to manage plant diseases naturally. In the future, we predict actinobacterial research is still essential to biological control, particularly those involving Streptomyces species. Also, several important topics are associated with their activities in controlling pests (nematode), postharvest disease, and growth promoter ability.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43492860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Purwanti, Julisa Ginti Mulyasari, Budianto Budianto
Fall armyworm or Spodoptera frugiperda is one of the main pests of corn. It can caused up to 28.08% yield loss. Pest control efforts with broad-spectrum insecticides can cause negative impacts that disrupt ecosystems, non-target biotic factors and the surrounding environment. Beauveria bassiana is an alternative biological control agent that is target-specific. This study was conducted to determine toxic and antifeedant activities of B. bassiana application on third to fifth-instar S. frugiperda larvae. B. bassiana isolates were propagated using corn flakes media and resulted in colonies with conidia density of 1.1×108conidia.g-1. This study used a completely randomized design with 5 treatments and 5 replications. Treatments consisted of P1 namely B. bassiana propagation with a concentration of 60 g.L-1, P2 = 70 g.L-1, and P3 = 80 g.L-1. P0 was a negative control (untreated with B. bassiana) and P4 was a positive control (diazinon with concentrations of 2 ml.L-1). The variables observed were mortality and weight of the leftover feed. The results showed that the formulation of B. bassiana with concentrations of 80 g.L-1 was able to infect armyworms with a mortality percentage of 53.5% which was statistically similar as the effect of chemical insecticides. In addition, inhibition of feeding activity due to treatment could prevent leaf damage up to 68.21%.
{"title":"In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae","authors":"E. Purwanti, Julisa Ginti Mulyasari, Budianto Budianto","doi":"10.22146/jpti.74940","DOIUrl":"https://doi.org/10.22146/jpti.74940","url":null,"abstract":"Fall armyworm or Spodoptera frugiperda is one of the main pests of corn. It can caused up to 28.08% yield loss. Pest control efforts with broad-spectrum insecticides can cause negative impacts that disrupt ecosystems, non-target biotic factors and the surrounding environment. Beauveria bassiana is an alternative biological control agent that is target-specific. This study was conducted to determine toxic and antifeedant activities of B. bassiana application on third to fifth-instar S. frugiperda larvae. B. bassiana isolates were propagated using corn flakes media and resulted in colonies with conidia density of 1.1×108conidia.g-1. This study used a completely randomized design with 5 treatments and 5 replications. Treatments consisted of P1 namely B. bassiana propagation with a concentration of 60 g.L-1, P2 = 70 g.L-1, and P3 = 80 g.L-1. P0 was a negative control (untreated with B. bassiana) and P4 was a positive control (diazinon with concentrations of 2 ml.L-1). The variables observed were mortality and weight of the leftover feed. The results showed that the formulation of B. bassiana with concentrations of 80 g.L-1 was able to infect armyworms with a mortality percentage of 53.5% which was statistically similar as the effect of chemical insecticides. In addition, inhibition of feeding activity due to treatment could prevent leaf damage up to 68.21%.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46250766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Spodoptera frugiperda is a new pest in Indonesia that attacks corn and can cause up to 100 percent damageon young plants. As an invasive pest, information on potential indigenous natural enemies that cancontrol this pest is needed. This study aims to determine the effectiveness of indigenous entomopathogenic fungi and their application methods on mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. This study used a factorial Completely Randomized Block Design (RCBD) method consisting of 10 treatments. Treatmentstested werecombination of fungi species, namely Fusarium sp., Aspergillus oryzae, Entomophthora sp. with conidia density 109 ml-1, distilled water and cypermethrin at concentration of 1 ml.L-1 as a control. Two application techniques used were direct application on S. frugiperda larvae and diet test. Each treatment was repeated three times resulting in30 experimental units. Variables observed included mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. Results showed that the three fungi and cypermethrin treatment did not cause mortality, but reduced feeding activity, fecundity and fertility of S. frugiperda. Application of entomopathogenic fungi ondietswas more effective than directly spraying S. frugiperda larvae. The best treatment combination that suppressed feeding activity was the application of Aspergillus oryzae sprayed on S. frugiperda diet.It was also suspected that S. frugiperda larvae used in this test had developed resistance to cypermethrin.
{"title":"Biological Effects of Indigenous Entomopathogenic Fungi and Their Application Methods on Spodoptera frugiperda","authors":"E. W. Minarni, Nurtiati Nurtiati, Dina Istiqomah","doi":"10.22146/jpti.70816","DOIUrl":"https://doi.org/10.22146/jpti.70816","url":null,"abstract":"Spodoptera frugiperda is a new pest in Indonesia that attacks corn and can cause up to 100 percent damageon young plants. As an invasive pest, information on potential indigenous natural enemies that cancontrol this pest is needed. This study aims to determine the effectiveness of indigenous entomopathogenic fungi and their application methods on mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. This study used a factorial Completely Randomized Block Design (RCBD) method consisting of 10 treatments. Treatmentstested werecombination of fungi species, namely Fusarium sp., Aspergillus oryzae, Entomophthora sp. with conidia density 109 ml-1, distilled water and cypermethrin at concentration of 1 ml.L-1 as a control. Two application techniques used were direct application on S. frugiperda larvae and diet test. Each treatment was repeated three times resulting in30 experimental units. Variables observed included mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. Results showed that the three fungi and cypermethrin treatment did not cause mortality, but reduced feeding activity, fecundity and fertility of S. frugiperda. Application of entomopathogenic fungi ondietswas more effective than directly spraying S. frugiperda larvae. The best treatment combination that suppressed feeding activity was the application of Aspergillus oryzae sprayed on S. frugiperda diet.It was also suspected that S. frugiperda larvae used in this test had developed resistance to cypermethrin.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46396014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yurista Sulistyawati, Y. Trisyono, Witjaksono Witjaksono
The brown planthopper, Nilaparvata lugens Stål. (Hemiptera: Delphacidae), is one of the most important pests attacking rice plants. One of the strategies to control this pest is by using natural enemies, including egg parasitoids. This study aimed to investigate the species of N. lugens egg parasitoids and their parasitism levels in one rice ecosystem with different growth stages. The research was conducted by selecting three rice plots aged 3, 6, and 10 weeks after planting. Potted rice plants with N. lugens eggs were placed in those rice plots for two days and recollected for observations. The same release was repeated 3 weeks later at the same rice plots in the same site. Egg parasitoids of N. lugens found during this study were Oligosita sp. and Anagrus sp. The highest number of egg parasitoids were found at the rice plants aged 6 weeks after planting with 14.50 and 13.50 individuals collected during first and second trapping, respectively. In addition, the level of parasitism ranged from 6.89-22.26% with the highest parasitism was occurred in the 6-week old rice. These findings may suggest that different rice planting dates may benefit the egg parasitoids of N. lugens which could reduce the risk of outbreaks.
{"title":"Brown Planthopper Egg Parasitization in Rice Fields with Different Growth Ages","authors":"Yurista Sulistyawati, Y. Trisyono, Witjaksono Witjaksono","doi":"10.22146/jpti.67448","DOIUrl":"https://doi.org/10.22146/jpti.67448","url":null,"abstract":"The brown planthopper, Nilaparvata lugens Stål. (Hemiptera: Delphacidae), is one of the most important pests attacking rice plants. One of the strategies to control this pest is by using natural enemies, including egg parasitoids. This study aimed to investigate the species of N. lugens egg parasitoids and their parasitism levels in one rice ecosystem with different growth stages. The research was conducted by selecting three rice plots aged 3, 6, and 10 weeks after planting. Potted rice plants with N. lugens eggs were placed in those rice plots for two days and recollected for observations. The same release was repeated 3 weeks later at the same rice plots in the same site. Egg parasitoids of N. lugens found during this study were Oligosita sp. and Anagrus sp. The highest number of egg parasitoids were found at the rice plants aged 6 weeks after planting with 14.50 and 13.50 individuals collected during first and second trapping, respectively. In addition, the level of parasitism ranged from 6.89-22.26% with the highest parasitism was occurred in the 6-week old rice. These findings may suggest that different rice planting dates may benefit the egg parasitoids of N. lugens which could reduce the risk of outbreaks. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41906461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A’isyah Surya Bintang, A. Wibowo, A. Priyatmojo, S. Subandiyah
Biodiversity has been defined as the range of significantly different types of organisms and their relative abundance in an assemblage of community. The aim of this research was to understand about soil bacterial community after on biological control agents (BCA) treatments with various formulations. This research was conducted at Sleman Regency, Special Region of Yogyakarta and Faculty Agriculture of Universitas Gadjah Mada. The research conducted with culture dependent and culture independent methods to assess soil bacterial diversity. The results showed that soil bacterial diversity before and after treatment of biological control agent were different. Results from this research suggested different molecular methods regarding soil bacterial diversity based on their benefits and challenges.
{"title":"The Effect of Biological Control Agents on Paddy Soil Bacterial Community Structure","authors":"A’isyah Surya Bintang, A. Wibowo, A. Priyatmojo, S. Subandiyah","doi":"10.22146/jpti.73197","DOIUrl":"https://doi.org/10.22146/jpti.73197","url":null,"abstract":"Biodiversity has been defined as the range of significantly different types of organisms and their relative abundance in an assemblage of community. The aim of this research was to understand about soil bacterial community after on biological control agents (BCA) treatments with various formulations. This research was conducted at Sleman Regency, Special Region of Yogyakarta and Faculty Agriculture of Universitas Gadjah Mada. The research conducted with culture dependent and culture independent methods to assess soil bacterial diversity. The results showed that soil bacterial diversity before and after treatment of biological control agent were different. Results from this research suggested different molecular methods regarding soil bacterial diversity based on their benefits and challenges. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46089839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Indonesian government is obliged to ensure the public health of the people of Indonesia by the supervision of Fresh Food Originated from Plant (Pangan Segar Asal Tumbuhan [PSAT]) arriving from abroad. Therefore, the regulation of Minister of Agriculture Number 88 in 2011 was issued to regulate inspection requirement of PSAT. There are some challenges in the implementation of these regulations until 2015, such as the unavailability of accredited laboratories, the lack of communication between stakeholders, and limited human resource or utensil within testing laboratories. Most of the testing laboratories appointed by the government are not yet accredited. Only 60% are able to perform tests mandated listed in The Regulation of Minister of Agriculture No. 88 the Year 2011. On the other hand, private laboratories accredited by ISO/IEC 17025 showed higher capacity by being able to perform 77.8 to 100% of mandated tests. The implementation challenges were later addressed by the revision of the regulation and release of issue Regulation 04/2015 in ref. The Regulation of Minister of Agriculture 13/2016. These regulations acknowledge Food Safety System of the state of origin or their administration of the Testing Laboratory Registration. In other words, they put forward the implementation of supervision in the country of origin reducing Indonesia’s reliability on providing high level of testing laboratories itself. The implementation of The Regulation of Minister of Agriculture 04/2015 in ref. the Regulation of Minister of Agriculture 13/2016 expected Quarantine Measures on the importation of PSAT to be more effective and efficient. Therefore, impact of the implementation of this new regulation would shorten delay periods for cargo containers in ports (dwelling time), reduce handling cost of containers, minimize laboratory testing fees, and reduce risk of rejection of imported products because of incompliance.
印尼政府有义务通过监督从国外运来的源自植物的新鲜食品（Pangan Segar Asal Tumbuhan[PSAT]）来确保印尼人民的公共健康。因此，农业部于2011年颁布了第88号法规，以规范PSAT的检查要求。在2015年之前，这些法规的实施存在一些挑战，例如没有经过认证的实验室，利益相关者之间缺乏沟通，以及测试实验室内的人力资源或器具有限。政府指定的大多数检测实验室尚未获得认证。只有60%的人能够进行2011年农业部第88号条例中规定的测试。另一方面，通过ISO/IEC 17025认证的私人实验室表现出更高的能力，能够进行77.8%至100%的强制测试。实施方面的挑战后来通过修订法规和发布2015年第04号法规（参见农业部第13/2016号法规）得到了解决。本条例承认原产国的食品安全体系或其检测实验室注册管理。换言之，他们提出在原产国实施监督，降低了印尼提供高水平检测实验室的可靠性。2015年4月4日农业部长条例的实施，参考2016年13月农业部长条例，预计PSAT进口检疫措施将更加有效。因此，这项新规定的实施将缩短集装箱在港口的延误期（停留时间），降低集装箱的装卸成本，最大限度地降低实验室检测费用，并降低因不符合规定而拒绝进口产品的风险。
{"title":"The Performance of Supporting Laboratories to the Implementation of the Regulation of Minister of Agriculture No. 88 the Year 2011 through Agricultural Quarantine Agency Class I Semarang","authors":"Heru Wahyupraja, S. Somowiyarjo, Y. Trisyono","doi":"10.22146/jpti.23130","DOIUrl":"https://doi.org/10.22146/jpti.23130","url":null,"abstract":"The Indonesian government is obliged to ensure the public health of the people of Indonesia by the supervision of Fresh Food Originated from Plant (Pangan Segar Asal Tumbuhan [PSAT]) arriving from abroad. Therefore, the regulation of Minister of Agriculture Number 88 in 2011 was issued to regulate inspection requirement of PSAT. There are some challenges in the implementation of these regulations until 2015, such as the unavailability of accredited laboratories, the lack of communication between stakeholders, and limited human resource or utensil within testing laboratories. Most of the testing laboratories appointed by the government are not yet accredited. Only 60% are able to perform tests mandated listed in The Regulation of Minister of Agriculture No. 88 the Year 2011. On the other hand, private laboratories accredited by ISO/IEC 17025 showed higher capacity by being able to perform 77.8 to 100% of mandated tests. The implementation challenges were later addressed by the revision of the regulation and release of issue Regulation 04/2015 in ref. The Regulation of Minister of Agriculture 13/2016. These regulations acknowledge Food Safety System of the state of origin or their administration of the Testing Laboratory Registration. In other words, they put forward the implementation of supervision in the country of origin reducing Indonesia’s reliability on providing high level of testing laboratories itself. The implementation of The Regulation of Minister of Agriculture 04/2015 in ref. the Regulation of Minister of Agriculture 13/2016 expected Quarantine Measures on the importation of PSAT to be more effective and efficient. Therefore, impact of the implementation of this new regulation would shorten delay periods for cargo containers in ports (dwelling time), reduce handling cost of containers, minimize laboratory testing fees, and reduce risk of rejection of imported products because of incompliance.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41764564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hertina Artanti, T. Joko, S. Somowiyarjo, S. Suryanti
Twisted disease caused by Fusarium spp. is one of the primary diseases on shallots with potency to cause enormous losses by causing stunting and bulb rot. One alternative disease control is the induction of plant resistance since the seed stage. The aim of this study was to determine the content of salicylic acid, jasmonic acid, and phenolic compounds of shallot bulb seeds coated with biological control agents as a resistance response to twisted disease. The shallot cultivar used was Crok kuning. The treatments used in this study were the type of biological control agents, including Rhizophagus intraradices and Trichoderma asperellum, combined with and seed coating application time (one month before planting and simultaneously at planting). Biological control agents in the form of powder formulation applied as seed coating material and seeds were stored for one month before planting. The results showed that application of biological agents delayed the occurrence of the twisted disease symptoms. The salicylic acid content from plant treated with R. intraradices at the time of planting was slightly higher than the control. The jasmonic acid content in T. asperellum plants treated at planting was higher than then control. Total phenolic content from plants treated with T. asperellum at planting time was higher than the control. In general, application of biological control agent as seed coat did not result in significant increase in salicylic acid, jasmonic acid nor the phenolic compounds, compared to the pathogen infected control.
{"title":"The Potential of Rhizophagus intraradices and Trichoderma asperellum to Induce Shallot Resistance against Twisted Disease","authors":"Hertina Artanti, T. Joko, S. Somowiyarjo, S. Suryanti","doi":"10.22146/jpti.70673","DOIUrl":"https://doi.org/10.22146/jpti.70673","url":null,"abstract":"Twisted disease caused by Fusarium spp. is one of the primary diseases on shallots with potency to cause enormous losses by causing stunting and bulb rot. One alternative disease control is the induction of plant resistance since the seed stage. The aim of this study was to determine the content of salicylic acid, jasmonic acid, and phenolic compounds of shallot bulb seeds coated with biological control agents as a resistance response to twisted disease. The shallot cultivar used was Crok kuning. The treatments used in this study were the type of biological control agents, including Rhizophagus intraradices and Trichoderma asperellum, combined with and seed coating application time (one month before planting and simultaneously at planting). Biological control agents in the form of powder formulation applied as seed coating material and seeds were stored for one month before planting. The results showed that application of biological agents delayed the occurrence of the twisted disease symptoms. The salicylic acid content from plant treated with R. intraradices at the time of planting was slightly higher than the control. The jasmonic acid content in T. asperellum plants treated at planting was higher than then control. Total phenolic content from plants treated with T. asperellum at planting time was higher than the control. In general, application of biological control agent as seed coat did not result in significant increase in salicylic acid, jasmonic acid nor the phenolic compounds, compared to the pathogen infected control.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47275490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The plant-parasitic nematode (PPN) is often not recognized as important limiting crop production in Indonesia. This is largely caused by non-specific and non-dramatic above-ground plant disease symptoms, their microscopic nature, and partly caused by inadequate demonstration of the economic importance of this hidden pathogen. However, change in agricultural practices to meet the ever-increasing food demand along with global climate change may increase the risk of PPNs on crop productivity in the future. This paper reviews PPN inventory in Indonesia during the last three decades. Thirty-three genera of PPNs were found to associate with 25 host plants. Some genera were present at the densities that are considered as damaging levels in other countries. Results among surveys are difficult to compare because of differences in crop cultivar surveyed, cultivation practices, sampling unit and method, and nematode extraction techniques. Lack of field supporting data did not permit a valid assessment of nematode risk on a particular crop. The first record of several quarantined species has been reported, but not all of them have been validated molecularly. Challenges and opportunities to improve the future field survey are presented in this paper.
{"title":"Plant Parasitic Nematodes in Agricultural Ecosystem of Indonesia","authors":"Chaerani Chaerani","doi":"10.22146/jpti.71037","DOIUrl":"https://doi.org/10.22146/jpti.71037","url":null,"abstract":"The plant-parasitic nematode (PPN) is often not recognized as important limiting crop production in Indonesia. This is largely caused by non-specific and non-dramatic above-ground plant disease symptoms, their microscopic nature, and partly caused by inadequate demonstration of the economic importance of this hidden pathogen. However, change in agricultural practices to meet the ever-increasing food demand along with global climate change may increase the risk of PPNs on crop productivity in the future. This paper reviews PPN inventory in Indonesia during the last three decades. Thirty-three genera of PPNs were found to associate with 25 host plants. Some genera were present at the densities that are considered as damaging levels in other countries. Results among surveys are difficult to compare because of differences in crop cultivar surveyed, cultivation practices, sampling unit and method, and nematode extraction techniques. Lack of field supporting data did not permit a valid assessment of nematode risk on a particular crop. The first record of several quarantined species has been reported, but not all of them have been validated molecularly. Challenges and opportunities to improve the future field survey are presented in this paper.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42052701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metarhizium anisopliae is one of the most frequently used insect pathogen fungi in controlling Oryctes rhinoceros. This research aims to learn the potency of fungus M. anisopliae against O. rhinoceros larvae growth and development at the laboratory. The research methods used are T-tests comprising seven treatments and three replications. Fungi were applied in all larvae instar stages starting from the pre-molting of the first larvae instar, post-molting of the second larvae instar, active second larvae instar, pre-molting of the second larvae instar, post-molting of the third larvae instar, active third larvae instar, and pre-pupae stage which were then compared with each instar’s own control. The result indicates that fungus M. anisopliae is capable of suppressing O. rhinoceros growth and development. The fungus induced highest mortality rate of 87% to the third instar larvae and lowest mortality rate of 27% to the pre-molting of the first instar larvae. The fungus also affected the duration of larval stage. At pre-molting of the third larvae instar treated with M. anisopliae, the larval duration was 40 days compared to that of control that took 135 days. At the post-molting of the third larvae instar, the larval duration was 25 days compared to that of control that took 120 days. At the third larvae instar, the larval duration was no more than 15 days compared to that of control that reached 110 days. At pre-pupal stage, the larvae only lasted for 6 days while at control, they were able to last for 15 days. The fungus also affected the success of larva development in becoming pupae in all O. rhinoceros larval stage. The lowest success rate was found in the post-molting of the third larvae instar treated with M. anisopliae with 7% compared to its control with 100% while the highest success rate was found in the pre-molting of the first larval instar with 47% compared to its control with 93%.
{"title":"The Potency of Metarhizium anisopliae in Disturbing Oryctes rhinoceros (Coleoptera: Scarabaeidae) Growth and Development","authors":"Ummi Maysaroh, E. Martono, T. Harjaka","doi":"10.22146/jpti.71755","DOIUrl":"https://doi.org/10.22146/jpti.71755","url":null,"abstract":"Metarhizium anisopliae is one of the most frequently used insect pathogen fungi in controlling Oryctes rhinoceros. This research aims to learn the potency of fungus M. anisopliae against O. rhinoceros larvae growth and development at the laboratory. The research methods used are T-tests comprising seven treatments and three replications. Fungi were applied in all larvae instar stages starting from the pre-molting of the first larvae instar, post-molting of the second larvae instar, active second larvae instar, pre-molting of the second larvae instar, post-molting of the third larvae instar, active third larvae instar, and pre-pupae stage which were then compared with each instar’s own control. The result indicates that fungus M. anisopliae is capable of suppressing O. rhinoceros growth and development. The fungus induced highest mortality rate of 87% to the third instar larvae and lowest mortality rate of 27% to the pre-molting of the first instar larvae. The fungus also affected the duration of larval stage. At pre-molting of the third larvae instar treated with M. anisopliae, the larval duration was 40 days compared to that of control that took 135 days. At the post-molting of the third larvae instar, the larval duration was 25 days compared to that of control that took 120 days. At the third larvae instar, the larval duration was no more than 15 days compared to that of control that reached 110 days. At pre-pupal stage, the larvae only lasted for 6 days while at control, they were able to last for 15 days. The fungus also affected the success of larva development in becoming pupae in all O. rhinoceros larval stage. The lowest success rate was found in the post-molting of the third larvae instar treated with M. anisopliae with 7% compared to its control with 100% while the highest success rate was found in the pre-molting of the first larval instar with 47% compared to its control with 93%.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42047373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：