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Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp. 芽孢杆菌抑制沙葱扭曲病的诱导抗性机制。
Pub Date : 2022-07-11 DOI: 10.22146/jpti.73198
Elfrida Indriani Reno Wulan, A. Wibowo, T. Joko, A. Widiastuti
Plant Growth Promoting Rhizobacteria has been known for its ability to induce plant resistance on shallot against twisted disease. Its ability as a bioprotectant agent is estimated to be comparable to the efficacy of Trichoderma which is currently widely used as a biological control agent.. This study aims to determine the content of jasmonic acid, salicylic acid, peroxidase, and disease suppression in shallot by application of Bacillus velezensis B-27, Bacillus cereus RC76, and application with combination of both rhizobacteria. The application was carried out  with tuber dipping for 30 minutes in each treatment with a bacterial density of 108 CFU mL-1. Application using Trichoderma was used as the comparison treatment, and the control plot was not given any treatment. Pathogen inoculation was carried out simultaneously as planting using Fusarium acutatum with a spore density of 106 CFU mL-1. The jasmonic and salicylic acids content was measured using the High-Performance Liquid Chromatography method, and the peroxidase content was determined using the spectrophotometric method. Disease suppression was measured at 10-day intervals. The results showed that treatment with Bacillus cereus RC76 increased jasmonic and salicylic acid levels, while application with Bacillus velezensis B-27 showed the highest level of peroxidase. Treatments with Bacillus spp. were able to suppress twisted disease by 72.2% to 100%. This study demonstrated that application Bacillus spp. suppressed twisted disease on shallot and increased the jasmonic and salicylic acid content as induced resistance mechanism against pathogens.
植物生长促进根际细菌以其诱导植物对葱抗扭病的能力而闻名。据估计,其作为生物保护剂的能力与木霉的功效相当,木霉目前被广泛用作生物控制剂。。本研究旨在通过应用威氏芽孢杆菌B-27和蜡状芽孢杆菌RC76,以及两种根际细菌联合应用,测定葱中茉莉酸、水杨酸、过氧化物酶的含量和对疾病的抑制作用。在细菌密度为108CFU mL-1的每次处理中,用块茎浸渍30分钟进行施用。应用木霉作为对照处理,对照区未进行任何处理。使用孢子密度为106CFU mL-1的尖孢镰刀菌在种植的同时进行病原体接种。用高效液相色谱法测定茉莉酸和水杨酸的含量,用分光光度法测定过氧化物酶的含量。每隔10天测量一次疾病抑制。结果表明,用蜡样芽孢杆菌RC76处理可提高茉莉酸和水杨酸的水平,而用威氏芽孢杆菌B-27处理则显示出最高水平的过氧化物酶。用芽孢杆菌处理能够抑制扭曲病72.2%至100%。本研究表明,应用芽孢杆菌抑制了小葱的扭曲病,并提高了茉莉酸和水杨酸的含量,这是诱导小葱对病原体产生抗性的机制。
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引用次数: 3
Decomposition of Wood by Termites in Different Types of Land Use 白蚁对不同土地利用类型木材的分解作用
Pub Date : 2022-07-11 DOI: 10.22146/jpti.69322
Sri Heriza, D. Buchori, I. Harahap, N. Maryana
Decomposition is an important process in nature, as it can break down organic matter into smaller particles and nutrients. Nutrients are returned to the environment and can be reused by other organisms. One important organism that plays a role in the decomposition process is termites because termites are able to decompose organic matter such as wood in all habitats. Different termite habitats can be seen in terms of different types of land use. Some types of land use, such as natural forests, secondary forests, palm oil plantations, and settlements, can be used as a test site for wood decomposition by termites. All types of land use can be found in Dharmasraya Regency, West Sumatra Province. The purpose of this study was to study the decomposition of wood by termites in several different types of land use. Four types of land use are selected from habitats with the least disturbed conditions to the extent of high habitat disturbances, namely natural forests, secondary forests, palm oil plantations, and settlements. The study was conducted in Dharmasraya Regency, West Sumatra Province. The research method uses pine wood bait (Pinus merkusii), measuring 1.9 cm x 1.9 cm x 25 cm. The wooden bait is winded for seven days, and the wood bait is weighed to gain its initial weight and measured moisture content. Furthermore, wood bait that has been installed is observed and re-weighed to gain final weight and measured water content. The results showed that deforestation in Dharmasraya, West Sumatra had an effect on reducing wood decomposition by termites. Five (5) termite species were found in wood bait, including Pericapritermes sp.1, Microtermes sp.1, Schedorhinotermes sp.1, Macrotermes gilvus, and Coptotermes curvignathus.
分解是自然界中一个重要的过程,因为它可以将有机物分解成更小的颗粒和营养物质。营养物质被返回到环境中,可以被其他生物再利用。在分解过程中起作用的一种重要生物是白蚁,因为白蚁能够分解所有栖息地的有机物质,如木材。根据不同的土地利用类型,可以看到不同的白蚁栖息地。某些类型的土地利用,如天然林、次生林、棕榈油种植园和定居点,可以用作白蚁分解木材的试验场。所有类型的土地使用都可以在西苏门答腊省的Dharmasraya Regency找到。本研究的目的是研究几种不同土地利用方式下白蚁对木材的分解。从受干扰程度最小的生境中选择了四种土地利用类型,即天然林、次生林、棕榈油种植园和定居点。这项研究是在西苏门答腊省Dharmasraya Regency进行的。研究方法采用松木诱饵(Pinus merkusii),尺寸为1.9 cm x 1.9 cm x 25 cm。将木制诱饵缠绕七天,并称重以获得其初始重量并测量水分含量。此外,观察已安装的木诱饵并重新称重以获得最终重量并测量含水量。结果表明,西苏门答腊Dharmasraya的森林砍伐对减少白蚁对木材的分解有影响。在饵料中发现白蚁5种,分别为大白蚁、小白蚁、小白蚁、大白蚁和弯白蚁。
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引用次数: 0
Parasitization Levels of Spodoptera frugiperda Eggs (Smith) (Lepidoptera: Noctuidae) in Three Different Corn Ecosystems in East Java 东爪哇三种不同玉米生态系统草地贪夜蛾卵(Smith)(鳞翅目:夜蛾科)的寄生水平
Pub Date : 2022-06-29 DOI: 10.22146/jpti.71598
Rizki Dwi Wahyuningsih, T. Harjaka, Suputa Suputa, Y. Trisyono
Spodoptera frugiperda  (Lepidoptera: Noctuidae) has successfully invaded and spread to almost all provinces of Indonesia which may cause significant impacts on corn production. Local natural enemies could play an important role in managing this invasive insect, and more diverse ecosystems would benefit natural enemies. This study aimed to assess parasitization rates of S. frugiperda eggs by local egg parasitoids in three different corn ecosystems (agroforestry, rice field, and rainfed field) in East Java. Sentinel egg masses were used for this study by exposing eight-hour-old egg masses collected from the laboratory mass-rearing and left for 24 hours in corn plantations aged 7, 14, and 28 days after planting. Telenomus sp. was more abundant in the three ecosystems compared to Trichogramma sp. The egg mass parasitization varied from 15.6 to 52.5%. The number of egg masses parasitized was consistently higher in agroforestry, followed by rice fields and rainfed fields in all three different sampling times. Interestingly parasitization rates on egg masses were not different, and they ranged from 43.7 to 81.6%. These findings provide evidence on the importance of local egg parasitoids for managing S. frugiperda and some insights related to plant diversity to improving the services by these parasitoids. 
草地贪夜蛾(鳞翅目:夜蛾科)已成功入侵并蔓延至印度尼西亚几乎所有省份,这可能对玉米生产造成重大影响。当地的天敌可以在管理这种入侵昆虫方面发挥重要作用,更多样的生态系统将有利于天敌。本研究旨在评估东爪哇三种不同玉米生态系统(农林、稻田和旱地)中本地卵寄生蜂对草地贪夜蛾卵的寄生率。哨兵卵块用于本研究,方法是暴露从实验室集体饲养中收集的8小时龄的卵块,并在种植后7、14和28天的玉米种植园中放置24小时。与赤眼蜂相比,Telenomus sp.在这三个生态系统中的数量更为丰富。卵团寄生率在15.6%至52.5%之间。在三个不同的采样时间内,农林业中的卵团寄生数量始终较高,其次是稻田和旱地。有趣的是,卵团的寄生率没有差异,从43.7%到81.6%不等。这些发现为当地卵寄生蜂对管理草地贪夜蛾的重要性提供了证据,并为改善这些寄生蜂的服务提供了与植物多样性相关的一些见解。
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引用次数: 1
Biology and Demography of Helopeltis bradyi Waterhouse (Hemiptera: Miridae) Reared on Cucumbers 黄瓜上饲养的布氏斑蝶的生物学和种群学
Pub Date : 2022-06-28 DOI: 10.22146/jpti.71761
Rich Gemilang Simanjuntak, T. Harjaka, A. Wijonarko
Helopeltis bradyi is often used as for research and is required in large numbers. The insects were obtained from cacao plants and reared with cucumber fruit in the laboratory. This study aimed to determine the biology and demography of H. bradyi reared on cucumber under laboratory conditions. The demographic parameters observed were net reproduction rate (Ro), gross reproduction rate (GRR), intrinsic growth rate (r), average generation period (T), and population doubling (DT). The results showed that the egg stage lasted for 6.33 ± 0.47 days, the developmental period of the I‒V instar nymph was 2.13 ± 0.34, 2.07 ± 0.25, 2.13 ± 0.34, 2.33 ± 0.47, and 3.20 ± 0.40 days. The lifespan of male imagoes were 32.33 ± 4.92 days, and female imagoes were 24.60 ± 6.64 days. The adult female pre-oviposition period was 2.2 ± 0.40 days and oviposition period of 22.9 ± 4.66 days. H. bradyi has a type III survival curve which indicated high mortality rates during the first instar egg and nymph population. Demographic statistics of H. bradyi on cucumbers fruit are GRR of 296 individuals per generation, Ro of 196 individuals per parent per generation, rate of r of 0.18 individuals per parent per day, T of  29.34 days, and DT of  3.85 days.
布氏海螺常用于研究,需要大量的海螺。这些昆虫是从可可植物中获得的,并在实验室与黄瓜果实一起饲养。本研究旨在确定在实验室条件下在黄瓜上饲养的布氏夜蛾的生物学和人口学特征。观察到的人口统计学参数为净繁殖率(Ro)、总繁殖率(GRR)、内在增长率(r)、平均生育周期(T)和种群倍增率(DT)。结果表明,卵期为6.33±0.47 d, I-V龄若虫的发育周期分别为2.13±0.34、2.07±0.25、2.13±0.34、2.33±0.47和3.20±0.40 d。雄象寿命为32.33±4.92 d,雌象寿命为24.60±6.64 d。成虫产卵前期为2.2±0.40 d,产卵期为22.9±4.66 d。布氏夜蛾具有III型生存曲线,表明在一龄卵和若虫种群中死亡率较高。黄瓜果实上布氏夜蛾的种群统计GRR为296只/代,Ro为196只/亲本/代,r为0.18只/亲本/天,T为29.34天,DT为3.85天。
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引用次数: 0
Application of Vermicompost with Different Feeding Material for Inducing Rice Plant Resistance against Brown Planthopper (Nilaparvata lugens Stål.) Attack 不同投料材料的粘虫复合物在水稻抗褐飞虱中的应用
Pub Date : 2021-12-31 DOI: 10.22146/jpti.60824
D. Hastuti, E. P. Ningsih, S. Ritawati, R. Khastini
Brown planthopper (BPH) is a major pest in rice plants and has become a global pest. This pest causes hopperburn in rice plants. This research aimed to know the effect of vermicompost from different feeding materials on rice plant damage by brown planthopper attack. This research was carried out in November 2019–March 2020 at the Sistandu integrated farming system, Serang City, Banten. The experiment method used in this research was a factorial Randomized Block Design (RBD) consisting of two factors with three replications. The first factor was vermicompost (P) 5 levels, while the second factor was rice varieties (V) with two groups so that there were ten treatments. Data were analyzed with ANOVA, then followed by 5% DMRT. The results showed that the application of vermicompost with different feeding materials gave non-significant results to the growth of rice plants in the vegetative phase and fecundity of the BPH. However, it significantly affected the intensity of rice plant damage in the generative phase, the offspring population of BPH, and the BPH feeding rate. While the use of two varieties of rice does not have a distinct effect on all parameters. There is no interaction effect between two factors, both vermicompost feeding material treatment and rice plant variety, to all the parameters that have been studied.
褐飞虱是水稻的主要害虫,已成为全球性害虫。这种害虫会引起水稻植株的跳花病。本研究旨在了解不同饲养材料的蚯蚓堆肥对褐飞虱对水稻植株伤害的影响。这项研究于2019年11月至2020年3月在万丹省瑟朗市Sistandu综合农业系统进行。本研究采用的实验方法是析因随机分组设计(RBD),由两个因素组成,三次重复。第一个因素是蚯蚓堆肥(P)5水平,而第二个因素是水稻品种(V),有两组,因此有十个处理。数据采用方差分析,然后采用5%DMRT。结果表明,施用不同饲养材料的蚯蚓粪对褐飞虱营养期植株的生长和繁殖力影响不大。然而,它显著影响了水稻生殖期的植株损伤强度、褐飞虱的后代数量和褐飞虱的取食率。而使用两个品种的水稻并不是对所有参数都有明显的影响。蚯蚓粪饲养材料处理和水稻品种两个因素对所研究的所有参数都没有交互作用。
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引用次数: 0
Characterization and Pathogenicity of Rhizoctonia solani Kühn Associated with Sheath Blight in Local Rice Varieties (Oryza sativa L.) of Special Region of Yogyakarta Yogyakarta特殊地区水稻品种(Oryza sativa L.)与纹章病相关的丝核菌Kühn的特性及致病性
Pub Date : 2021-12-17 DOI: 10.22146/jpti.67557
Maulin Nafisa, Adiana Nayogyani, R. Kasiamdari
In Indonesia, rice is used as the first strategic food commodity and a priority in agricultural development. However, in practice, efforts to increase it still experience various obstacles, one of which is caused by the attack of pathogen Rhizoctonia solani. This study aimed to determine the characters of R. solani and observe its pathogenicity on some local rice varieties in the Special Region of Yogyakarta. Four isolates of R. solani were used, namely RS MS2, RS MKP2, RS UMB, and RS PN, as well as six varieties of local rice varieties, which were Menthik Susu, Cempo Merah, Cempo Hitam, Segreng, Menoreh, and Hitam Makaryo, and national rice varieties IR 64 and Ciherang. Morphological characterization was carried out based on the cultural characteristics of the colony, hyphae, and sclerotia. A pathogenicity test was carried out using the Detached Leaf Inoculation method. The result showed that R. solani characters vary from whitish-brown, yellowish-brown, and light brown colonies; moderate and fast growth rates; abundant-aerial, moderate-aerial, and slight-aerial growth patterns; 90° hyphae branching, narrowing of hyphae at the branching point, and presence of sclerotia. The pathogenicity test gave the results of all isolates having varying pathogenicity levels on each rice variety. Menthik Susu, Cempo Hitam, and Hitam Makaryo were resistant to R. solani. Cempo Merah and Segreng were considered as moderately resistant varieties, whereas Menoreh was categorized as moderately susceptible. Ciherang and IR64 were susceptible varieties to R. solani.
在印度尼西亚,大米被用作第一种战略粮食商品,也是农业发展的优先事项。然而,在实践中,增加它的努力仍然遇到各种障碍,其中一个障碍是由病原体立枯丝核菌的攻击引起的。本研究旨在确定龙葵的特性,并观察其对日惹特别地区一些地方水稻品种的致病性。使用了4个分离株,即RS MS2、RS MKP2、RS UMB和RS PN,以及6个地方水稻品种,即Menthik Susu、Cempo Merah、Cempo-Hitam、Segreng、Menoreh和Hitam Makaryo,以及国家水稻品种IR 64和Ciherang。根据菌落、菌丝和菌核的培养特征进行形态学表征。采用离体叶片接种法进行了致病性试验。结果表明,龙葵的性状有白褐色、黄褐色和浅褐色菌落;中高速增长率;丰富的气生、适度的气生和轻微的气生生长模式;90°菌丝分枝,分枝点菌丝变窄,有菌核。致病性试验给出了所有菌株对每个水稻品种具有不同致病性水平的结果。明提克·苏苏、Cempo Hitam和Hitam Makaryo对R.solani具有抗性。Cempo-Merah和Segreng被认为是中度抗性品种,而Menoreh被归类为中度敏感品种。Ciherang和IR64是茄尼感病品种。
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引用次数: 0
Arthropod and Earthworm Populations on Irrigated Rice Farming Land after Paraquat Herbicide Application 施用百草枯除草剂后灌溉稻田节肢动物和蚯蚓种群
Pub Date : 2021-12-13 DOI: 10.22146/jpti.54363
E. Martono, Ruwinda Wahyu Wijayanti, Kusnanik Kusnanik, D. V. Torani
Paraquat dichloride is a herbicide compound commonly used to control grasses and broadleaf weeds. Its use is presently under scrutiny as there is a report that this herbicide is very harmful and hazardous to the environment, especially to wild fauna surrounding the farming sites. An irrigated rice farming locale experiment was conducted to observe the effect of paraquat treatment on rice plant biotic environment, particularly its arthropods and earthworm population, two of the most prominent fauna easily affected by paraquat. The study was carried out in Seyegan District, Sleman Regency, Special Region of Yogyakarta Province, on irrigated rice land from October 2018 to February 2019. Four treatments of paraquat, i.e. control (0 kg/ha paraquat), lower dose (0.8 kg/ha), medium dose (1.6 kg/ha), and higher dose (3.2 kg/ha) were arranged. Carbendazim 5 kg/ha was also applied as comparing pesticides, especially for paraquat’s effect on the earthworm. The treatments were replicated five times in a plot of 5 x 5 m2. The number of arthropods and earthworms was observed in about two weeks intervals or more. The observation was done for the number of arthropods and their families, while for earthworms, only for their numbers. The arthropods population data were subjected to diversity indices and statistical analysis, while the earthworms data were statistically analyzed. The result showed that the arthropod numbers in treated plots were statistically significantly different, but biologically their number was still abundant. Their diversity did not differ from the control treatment at all. The arthropod numbers were 2007, 1483, 1095, 1746, and 1204 in control, lower, medium, higher, and carbendazim plots. The earthworms' numbers were still plenty; those in the higher dosage did not differ significantly from those in control. Their numbers from control, lower, medium, higher dose, and carbendazim plots were 811, 658, 567, 882, and 445 individual earthworms. 
百草枯二氯化物是一种常用于控制草和阔叶杂草的除草剂化合物。目前正在对其使用进行审查,因为有报告称,这种除草剂对环境非常有害,尤其是对农场周围的野生动物非常有害。通过稻田灌溉试验,观察了百草枯处理对水稻植物生物环境的影响,特别是对其节肢动物和蚯蚓种群的影响。这项研究于2018年10月至2019年2月在日惹省特别地区Sleman Regency Seyegan区的灌溉稻田上进行。安排了四种百草枯处理,即对照组(0公斤/公顷百草枯)、低剂量组(0.8公斤/公顷)、中剂量组(1.6公斤/ha)和高剂量组(3.2公斤/哈)。多菌灵5kg/ha也被用作比较农药,特别是百草枯对蚯蚓的影响。在5 x 5 m2的地块上重复处理5次。节肢动物和蚯蚓的数量是在大约两周或更长的时间间隔内观察到的。对节肢动物及其家族的数量进行了观察,而对蚯蚓的观察仅针对其数量。对节肢动物种群数据进行多样性指数和统计分析,对蚯蚓数据进行统计分析。结果表明,处理小区节肢动物数量在统计学上有显著差异,但在生物学上其数量仍然丰富。它们的多样性与对照组没有任何差异。对照、低、中、高和多菌灵小区的节肢动物数量分别为2007、1483、1095、1746和1204。蚯蚓的数量仍然很多;高剂量组与对照组没有显著差异。对照、低剂量、中剂量、高剂量和多菌灵小区的蚯蚓数量分别为811、658、567、882和445条。
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引用次数: 0
Susceptibility of Two Varieties of Guava (Psidium guajava L.) to Pest and Disease Infection in the Sub-District of Tanah Sareal, Bogor 两种番石榴品种在茂物塔那沙雷街道对病虫害的敏感性
Pub Date : 2021-12-10 DOI: 10.22146/jpti.49851
H. Triwidodo, E. T. Tondok, Desy Nur Avifah
This survey was done in several guava fields around Bogor and found many unhealthy plants infested by various plant pests and diseases. This study aimed to analyze the effect of various guava cultivars on pest and disease intensity on guava in the Sub-District of Tanah Sareal, Bogor. Samples were collected from 20 fields that consisted of 15 samples of Getas Merah and 5 samples of Bangkok Putih cultivars to identify plant pests and diseases. Interviews were done with field owners to obtain information on cultivating practices. Pests found based on morphological identification included plant bugs (Hemiptera: Miridae), locusts (Orthoptera: Acrididae), bagworms (Lepidoptera: Psychidae), Geometrid caterpillars (Lepidoptera: Geometridae), Pyralid caterpillars (Lepidoptera: Pyralidae), scale insects (Hemiptera: Coccidae), planthoppers (Hemiptera: Flatidae), and mealybugs (Hemiptera: Pseudococcidae). Disease and pathogens identified included red rust disease (Cephaleuros sp.), anthracnose (Colletotrichum gloeosporioides), and fruit cancer (Pestalotia sp.). The occurrence of biting-chewing type pests was greater than piercing-sucking type insects, including 33.33% and 34.99%. Red rust disease was the dominant disease found on both guava cultivars consisting of 68.88% and 63.33% of the samples. 
这项调查在茂物周围的几块番石榴地进行,发现许多不健康的植物受到各种植物病虫害的侵扰。本研究旨在分析不同番石榴品种对茂物Tanah Sareal分区番石榴病虫害强度的影响。从20块田地采集样本,其中包括15个Getas Merah和5个Bangkok Putih品种样本,以识别植物病虫害。对田地所有者进行了访谈,以获得有关耕作做法的信息。根据形态学鉴定发现的害虫包括植物虫(半翅目:Miridae)、蝗虫(直翅目:Acrididae。所鉴定的病害和病原体包括红锈病(Cephaleuros sp.)、炭疽病(Colletotrichum gloesporioides)和果实癌症(Pestalotia sp.)。咬食型害虫的发生率高于刺食型害虫,分别为33.33%和34.99%。红锈病是两个番石榴品种的主要病害,占样品的68.88%和63.33%。
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引用次数: 0
Diversity of Beneficial Insect in Corn Plantation at West Sumatra 西苏门答腊玉米种植园有益昆虫的多样性
Pub Date : 2021-12-09 DOI: 10.22146/jpti.61254
Martinus Abuzar, U. Khairul, H. Hamid
The corn plant is one of Indonesia's agricultural products with economical and strategic value and many potential to develop, such as the main carbohydrates and protein source. There are two types of beneficial insects in corn planting: predators and parasitoids. This study aims to know the diversity of beneficial insects in corn plantations. It was conducted from May – September 2019 in four different locations across West Sumatra, namely Padang Pariaman, West Pasaman, Pesisir Selatan, Solok Regencies. Identification of beneficial insects was made in Entomology Laboratory, Faculty of Agriculture, Andalas University. This study adopted a purposive sampling method using swing net 20 times, following the transect line of corn planting. The result showed five orders, 34 families, 101 morphospecies, and 747 beneficial insects in all locations. The diversity in each place varies from high, medium, and low. In all areas, the diversity index was high, with the criteria of ≥ 2.90, which means that the condition of the community structure was very stable. From the evenness index analysis, three locations were classified as high, and the other one was moderate. These three sites were Padang Pariaman, West Pasaman, and Solok, with criteria of ≥ 0.90 and very stable distribution conditions. 
玉米是印尼具有经济和战略价值的农产品之一,具有许多开发潜力,如主要的碳水化合物和蛋白质来源。玉米种植中有两种益虫:捕食者和寄生性昆虫。本研究旨在了解玉米种植园中有益昆虫的多样性。该调查于2019年5月至9月在西苏门答腊岛的四个不同地点进行,即巴东帕里亚曼、西帕萨曼、佩西西尔塞拉坦、索洛克摄政区。有益昆虫鉴定由安达拉斯大学农学院昆虫学实验室进行。本研究采用摆网20次有目的取样法,沿玉米种植样条线取样。结果表明,各生境共有5目34科101种,益虫747种。每个地方的多样性从高、中、低不等。各地区群落多样性指数均较高,分值≥2.90,表明群落结构状况稳定。从均匀度指数分析来看,有3个地点被划分为高,另一个被划分为中等。这3个地点分别为Padang Pariaman、West Pasaman和Solok,标准≥0.90,分布条件非常稳定。
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引用次数: 2
Double Infections of Rehmannia mosaic virus and Potato virus Y on Tobacco Plants in Central Java and Special Region of Yogyakarta 地黄花叶病毒和马铃薯Y病毒对爪哇中部和日惹特别地区烟草的双重感染
Pub Date : 2021-12-09 DOI: 10.22146/jpti.67468
Tika Pramudya Wardani, S. Hartono, S. Sulandari, S. Somowiyarjo
Various viruses can cause mosaic disease in tobacco plants. Proper detection of the cause of the mosaic disease helps determine effective control. The purpose of this study was to detect the presence of double infection of Rehmannia mosaic virus (ReMV) with Potato virus Y (PVY) using multiplex RT-PCR in tobacco plants from Central Java and Special Region of Yogyakarta. The viral suspension was inoculated on Chenopodium amaranticolor to obtain one viral colony from one local lesion. The multiplex RT-PCR method using Tobamovirus primers (TobRT-up1 and TobRT-do2) and Potyvirus primers (MJ1 and MJ2) can detect double infection caused by ReMV with PVY in tobacco plants distributed in Central Java and Special Region of Yogyakarta. The multiplex RT-PCR product showed that tobacco samples with mosaic symptoms from Temanggung, Klaten, Bantul, and Kalasan were positive ReMV. Multiplex RT-PCR has successfully detected double infection of ReMV and PVY on tobacco samples from Klaten and Kalasan. ReMV Bantul, Kalasan, and Klaten were homolog to ReMV USA isolate and ReMV Temanggung was homolog to ReMV Japanese isolate. PVY Klaten was homolog to PVY Turkey isolate, and PVY Kalasan was homolog to PVY Iran. 
各种病毒可引起烟草植物的花叶病。正确检测马赛克病的病因有助于确定有效的控制措施。本研究的目的是利用多重RT-PCR检测中爪哇岛和日惹特别地区烟草植物中是否存在地黄花叶病毒(ReMV)与马铃薯Y病毒(PVY)的双重感染。将病毒悬浮液接种在藜麦上以从一个局部病变获得一个病毒集落。使用Tobamovirus引物(TobRT-up1和TobRT-do2)和Potyvirus引物。多重RT-PCR产物显示,来自Temanggung、Klaten、Bantul和Kalasan的具有马赛克症状的烟草样品ReMV呈阳性。多重RT-PCR已成功检测到来自Klaten和Kalasan的烟草样品中ReMV和PVY的双重感染。ReMV Bantul、Kalasan和Klaten与ReMV美国分离株同源,ReMV Temanggung与ReMV日本分离株同源。PVY-Klaten与PVY-Turkey分离株同源,PVY-Kalasan与PVY Iran同源。
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引用次数: 0
期刊
Jurnal Perlindungan Tanaman Indonesia
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