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External Environmental and Insects Factor for Attraction of Edible Bird Nest Swiftlet (Aerodramus fuciphagus) in Sarawak 沙捞越食用燕窝金丝燕(Aerodramus fuciphagus)受外部环境和昆虫吸引的因素
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-29 DOI: 10.33736/bjrst.5787.2023
Amos Alvin, Hakim Idris Lokman, Abu Jalila, Md Isa Nur Mahiza, Emmanuel Busayo Ibitoye
Edible Bird Nest (EBN) swiftlet industry plays a significant role in the economic growth in Sarawak. This study aimed at identifying both the external environmental factors and types of insects that are important for a successful swiftlet farmhouse (SFH). For this purpose, a total of 21 SFHs from the southern areas of Sarawak were selected. The external environmental factors of SFH and the type of insects present in relation to the number of EBN swiftlet populations were analysed using descriptive statistics. The results showed that external environmental locations, namely rural and remote areas, were highly selected (71.42%) for the construction of SFHs (p<0.05) compared to the other location categories, as analysed using the Kruskal-Wallis test. Significant relationships (p<0.05) were observed between environmental factors (habitat origin and predation) and the population of EBN swiftlets in the SFHs. Large densities of the EBN swiftlet populations were found in SFHs located in areas with fewer disturbances. The highest population of insect types in most SFHs was Diptera (52.38%). This study can be used as a guideline for swiftlet ranchers looking to startup their EBN swiftlet farming.
食用燕窝(EBN)燕业在砂拉越的经济增长中扮演着重要角色。这项研究的目的是找出对成功的金丝燕农舍(SFH)非常重要的外部环境因素和昆虫种类。为此,研究人员从砂拉越南部地区挑选了21个农舍。研究人员利用描述性统计分析了农舍的外部环境因素和昆虫种类与EBN金丝燕种群数量的关系。结果显示,与其他地点类别相比,外部环境地点,即农村和偏远地区,被高度选择(71.42%)用于建造自力更生住房(p<0.05),这是用Kruskal-Wallis检验法进行分析的结果。环境因素(栖息地来源和捕食)与自力更生住房中的EBN燕数量之间存在显著关系(p<0.05)。在干扰较少的自力更生住房中发现了大量的金丝燕种群。大多数自力更生住房中数量最多的昆虫种类是双翅目(52.38%)。这项研究可作为养燕业者开始饲养EBN金丝燕的指南。
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引用次数: 0
Optimisation of Phytate Degradation in Whole Grain Rice During Germination Processing Using Response Surface Methodology 利用响应面方法优化整粒稻米在发芽处理过程中的植酸降解过程
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-28 DOI: 10.33736/bjrst.5347.2023
H. Lee, Elisha Yiu, ALVIN-LIM-TEIK Zheng, JOSEPH-CHOON-FAH Bong, SU-PENG Loh, PANG-HUNG Yiu
Phytic acid (IP6), stored in seeds as metal salts known as phytates, binds to micronutrients and prevents its absorption by the human body. The germination process could improve cereal nutritional values by stimulating endogenous phytase activity and promoting phytate degradation. This study evaluated the physicochemical changes of phytates in rice cultivars with different IP6 contents, followed by optimisation of phytate degradation using response surface modeling. The magnitude of changes in IP6 content and phytase activity differed among rice cultivars. This suggested that the efficiency of germination treatments relied on the amount of natural phytic acid and phytase activity present in the rice grains. The cultivar “Tuan” was then selected and studied for the germination effect on phytate degradation using a central composite design. The cultivar gave a lower IP6 content, enhanced phytase activity and improved minerals bioaccessibility under acidic conditions. Acidic germination facilitated the degradation of phytate complexes in whole grain rice by making phytate complexes more soluble, accelerating phytase activity and thus, releasing mineral micronutrients from phytate globoids. The optimum germination condition was identified at pH 2.7, 25 °C over 12 h. In conclusion, germination processing facilitated phytate degradation in whole grain rice to make value-added rice products with low phytic acid and good mineral bioaccessibility.
植酸(IP6)以被称为植酸盐的金属盐形式储存在种子中,会与微量营养素结合,阻止人体吸收。发芽过程可以刺激内源植酸酶的活性,促进植酸降解,从而提高谷物的营养价值。本研究评估了 IP6 含量不同的水稻品种中植酸的理化变化,并利用响应面模型对植酸降解进行了优化。不同水稻品种的 IP6 含量和植酸酶活性的变化幅度不同。这表明,发芽处理的效率取决于稻谷中天然植酸的含量和植酸酶的活性。因此,我们选择了 "Tuan "这一栽培品种,并采用中心复合设计法研究了发芽对植酸降解的影响。在酸性条件下,该品种的 IP6 含量较低,植酸酶活性增强,矿物质的生物利用率提高。酸性发芽能使植酸复合体更易溶解,加速植酸酶的活性,从而促进植酸复合体在整粒稻米中的降解,释放植酸球蛋白中的矿物质微量元素。总之,发芽处理可促进整粒大米中植酸的降解,从而生产出植酸含量低、矿物质生物可利用性好的高附加值大米产品。
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引用次数: 0
Assessment of Toxic Metals in Commonly Used Energy-Stimulating Herbal Drugs Manufactured in Rajshahi City, Bangladesh 对孟加拉国拉杰沙希市生产的常用能量刺激草药中有毒金属的评估
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-28 DOI: 10.33736/bjrst.5530.2023
MAMUN- AR-RASHID, R. Zamir, Nazmul Islam, Hasan Ahmed, Choudhury M. Zakaria
Having been a common healthcare distress, erectile dysfunction is upsetting the quality of life of men from all walks of life. Along with allopathic therapy, a sizable number of patients opt for energy-stimulating herbal drugs (ESHDs) to treat the ailment. However, a global threat vestige for metal contamination in plant-based drugs at above threshold concentrations. Investigation into metal toxicity through samples from Bangladesh is scarce. Six metals: chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), and zinc (Zn) were probed in 25 energy stimulating herbal drug samples by flame atomic absorption spectrophotometer (F-AAS). Metal content was below detection limit, 51.86 ± 0.07 ppm for Cr, below detection limit, 7.26 ± 0.07 ppm for Cu, 3.41 ± 0.14 – 59.00 ± 0.09 ppm for Fe, 3.54 ± 0.09 – 26.16 ± 0.04 ppm for Mn, below detection limit to 67.34 ± 0.58 ppm for Pb, and below detection limit 27.79 ± 0.07 ppm for Zn. Exposure assessment found Cr and Pb concentrations in the objectionable limits in 12% and 20% of the samples, respectively. This study signifies concern metal toxicity in ESHDs which demands additional probes in the future for other samples to guarantee safe consumption of the drugs.
勃起功能障碍是一种常见的健康问题,它正在扰乱各行各业男性的生活质量。除了对抗疗法外,相当多的患者还选择使用能量刺激草药(ESHDs)来治疗这种疾病。然而,植物类药物中的金属含量超过阈值浓度,存在全球性威胁。通过孟加拉国的样本对金属毒性进行的调查很少。研究人员利用火焰原子吸收分光光度计(F-AAS)对 25 种激发能量的草药样本中的六种金属:铬(Cr)、铜(Cu)、铁(Fe)、锰(Mn)、铅(Pb)和锌(Zn)进行了检测。铬的金属含量低于检测限(51.86 ± 0.07 ppm),铜的金属含量低于检测限(7.26 ± 0.07 ppm),铁的金属含量低于检测限(3.41 ± 0.14 - 59.00 ± 0.09 ppm),锰的金属含量低于检测限(3.54 ± 0.09 - 26.16 ± 0.04 ppm),铅的金属含量低于检测限(67.34 ± 0.58 ppm),锌的金属含量低于检测限(27.79 ± 0.07 ppm)。暴露评估发现,分别有 12% 和 20% 的样本中铬和铅的浓度达到了不良限值。这项研究表明,易制毒化学品中的金属毒性值得关注,今后需要对其他样品进行更多的检测,以确保药物的安全食用。
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引用次数: 0
Risk Screening of Introduced African Catfish (Clarias gariepinus) (Burchell, 1822) in Sarawak Using the Fish Invasiveness Screening Kit (FISK v2) 使用鱼类入侵性筛选工具包(FISK v2)对沙捞越引进的非洲鲶鱼(Clarias gariepinus)(Burchell,1822 年)进行风险筛选
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-28 DOI: 10.33736/bjrst.5705.2023
Chin Han, Kiat, K. Adha, A. Rahim
African Catfish (Clarias gariepinus) is a popular non-native fish for aquaculture in Malaysia. The issues of non-native fish species have not been much discussed despite general perception of the negative effects of the species on the native biodiversity. Therefore, this study was conducted to estimate the possible risk of C. gariepinus in Sarawak using a semi-quantitative system of Fish Invasiveness Screening Kit version 2 (FISK v2). There are 49 questions in FISK v2 assessment which was assessed by three independent assessors with fisheries knowledge in Sarawak. Threshold was set at 19.0. Descriptive Statistics using SPSS 25.0 was used to run FISK score from three assessors. Result shows that Clarias gariepinus was categorised as “very high risk”. Clarias gariepinus has a FISK score of 43.00 ± 1.00 with a certainty factor of 0.89 ± 0.08. Environmental and biological criteria, followed by the economic impacts for this species and the gaps in legislation and framework in Sarawak were discussed thoroughly. It can be concluded that this preliminary assessment might have indicate a sign of invasion of this non-native species to the local biodiversity. The tool could be more robust if more comprehensive data are included which eventually be useful to assist in decisions regarding future management of non-native species in Sarawak.
非洲鲶鱼(Clarias gariepinus)是马来西亚水产养殖业中一种流行的非本地鱼类。尽管人们普遍认为非本地鱼类物种会对本地生物多样性造成负面影响,但有关非本地鱼类物种问题的讨论并不多。因此,本研究使用鱼类入侵筛选工具包第 2 版(FISK v2)的半定量系统来估计沙捞越鲤鱼的可能风险。FISK v2 评估共有 49 个问题,由三位具有沙捞越渔业知识的独立评估员进行评估。阈值设定为 19.0。使用 SPSS 25.0 进行描述性统计,计算三位评估员的 FISK 分数。结果显示,石斑鱼被归类为 "极高风险"。珊瑚鱼的 FISK 得分为 43.00 ± 1.00,确定系数为 0.89 ± 0.08。对环境和生物标准以及该物种的经济影响和沙捞越立法与框架的不足进行了深入讨论。可以得出的结论是,这一初步评估可能表明了这种非本地物种入侵当地生物多样性的迹象。如果纳入更全面的数据,该工具将更加强大,最终将有助于沙捞越未来管理非本地物种的决策。
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引用次数: 0
A New Record of Panulirus homarus homarus (Panulirus spp.) from Malaysia Waters with its Molecular Phylogeny 来自马来西亚水域的 Panulirus homarus homarus (Panulirus spp.) 新记录及其分子系统发育
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-28 DOI: 10.33736/bjrst.6033.2023
WEI-LING Ng, CHENG-ANN Chen, S. Teng, A. D. Tuzan, M. N. Mullungal, TIN-YAM Chan
Spiny lobsters Panulirus spp. of the family Palinuridae Latreille, 1802 occupies the shallow tropical and subtropical waters (<100 m depth). These species are ubiquitous and abundant, yet knowledge of its range, biodiversity, and population abundance in Malaysia is limited. Panulirus homarus is listed as “Least Concern” along with other 11 species out of 21 spiny lobster species in the International Union for Conservation of Nature and Natural Resources (IUCN) red list in 2022. A total of five species were reported from Malaysia waters. Whilst, this study reports the sixth spiny lobster species, Panulirus homarus homarus, with morphology and molecular genetic information (Cytochrome oxidase I (COI) gene) provided. The p-distance within P. h. homarus was 0.2 – 0.6%, n = 12; between P. h. homarus with P. h. rubellus and P. homarus “Brown” was 6.4 – 6.8%, n = 12 and 0.8 – 3.3%, n = 12, respectively. Intra-species p-distance within Panulirus spp. was below 3%, while inter-species was above 10%. This validates the collected specimen as P. h. homarus and P. h. rubellus is the subspecies, but leaving P. h. “Brown” taxonomic status unclear. Thus, genetic information is important in solving many of these systematic and taxonomic problems, because often these species are difficult to distinguished based on morphological criteria. A key to all Panulirus spp. identified in Malaysia waters was constructed. This study updates the marine biodiversity list in Malaysia, where the acquisition of biological knowledge is vital to the protection of the world’s natural resources as well as to the sustainable management of spiny lobster fisheries.
棘龙虾(Palinuridae Latreille, 1802)科棘龙虾属(Panulirus spp.)生活在热带和亚热带浅海水域(水深小于 100 米)。这些物种无处不在,数量丰富,但对其在马来西亚的分布范围、生物多样性和种群数量的了解却很有限。2022 年,在国际自然及自然资源保护联盟(IUCN)的红色名录中,Panulirus homarus 与 21 个棘龙虾物种中的其他 11 个物种一起被列为 "最不关注 "物种。马来西亚水域共报告了 5 个物种。本研究报告了第六个棘龙虾物种--Panulirus homarus homarus,并提供了形态学和分子遗传信息(细胞色素氧化酶 I(COI)基因)。P. h. homarus内部的p-距离为0.2 - 0.6%,n = 12;P. h. homarus与P. h. rubellus和P.Panulirus属种内的p-距离低于3%,而种间的p-距离高于10%。这验证了采集到的标本为 P. h. homarus,P. h. rubellus 为亚种,但 P. h. "Brown" 的分类地位尚不明确。因此,遗传信息对于解决许多系统学和分类学问题非常重要,因为根据形态学标准,这些物种往往难以区分。本研究构建了一个在马来西亚水域发现的所有 Panulirus spp.的检索表。这项研究更新了马来西亚的海洋生物多样性清单,在马来西亚,获取生物知识对于保护世界自然资源和棘龙虾渔业的可持续管理至关重要。
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引用次数: 0
Synthesis, in vitro and in silico Studies of Alkylated Paracetamol Derivatives as Potential Antibacterial Agent 作为潜在抗菌剂的烷基化扑热息痛衍生物的合成、体外和硅学研究
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-28 DOI: 10.33736/bjrst.5935.2023
Ainaa Nadiah, Abd Halim, Aina Syakirah, Mohammad Hussin, Z. Ngaini, Ngui Sing, Stephanie Alma Jong, Muhammad Muqri, Mohamad Saberi
In the field of drug development, structural modification of over-the-counter drugs is extensive to combat drug-resistant strains as well as discover new purposes or biological applications. Paracetamol is an example of OTC analgesic drug that is commonly used in the treatment of pain and fever. In this research, paracetamol was modified by introducing various alkyl chains C 𝑛 H 2 𝑛 +1 , where 𝑛 varied from 3 to 14 via Williamson etherification with bromoalkanes to yield 49% – 86% of N -(4-oxy)phenyl)acetamide derivatives 1-10 . Employing the Kirby-Bauer disc diffusion method, the bacteriostatic activity of the series in comparison to the parental compound (paracetamol) and standard drug (ampicillin) showed that compounds 1 ( n = 3), 4 ( n = 6) and 7 ( n = 9) displayed excellent activity against Escherichia coli with an inhibition zone of 8.1 ± 0.4 mm, 8.3 ± 0.4 mm and 7.9 ± 0.5 mm, respectively, while the paracetamol and ampicillin inhibition zones are 8.0 ± 0.0 mm and 16.0 ± 0.0 mm, respectively. Interestingly, compounds 7 ( n = 9) and 10 ( n = 14) exhibited excellent activity towards Staphylococcus aureus with an inhibition zone of 8.2 ± 0.4 mm and 12.5 ± 0.5 mm, respectively, outperforming paracetamol (6.0 ± 0.0 mm) and ampicillin (11.0 ± 0.0 mm). The in vitro findings were in line with the molecular docking investigation conducted using AutoDock Vina software towards the active site of DNA gyrase enzymes, revealing the structure-activity relationship of the series. Besides, the synthesised compounds 1-10 complied with the drug-likeness analysis and ADME pharmacokinetics profile. The results of this study are significant in delivering new prospects of the potential antibacterial drugs obtained through derivatisation of the known drug, paracetamol. While these initial results are promising, further toxicity and in vivo analyses are necessary to ensure the efficacy and safety of the newly developed drug in living organisms, providing a clearer understanding of its potential as an antibacterial medicine.
在药物开发领域,对非处方药进行结构改造的做法非常广泛,以对抗耐药菌株,并发现新的用途或生物应用。扑热息痛就是一种非处方药物,常用于治疗疼痛和发烧。在这项研究中,通过威廉姆森与溴烷烃的醚化反应,引入各种烷基链 C 𝑛 H 2 𝑛 +1 对扑热息痛进行改性,其中𝑛从 3 到 14 不等,得到 49% - 86% 的 N -(4-oxy)phenyl)acetamide 衍生物 1-10。采用 Kirby-Bauer 盘扩散法,与母体化合物(扑热息痛)和标准药物(氨苄西林)相比,该系列化合物的抑菌活性表明,化合物 1(n = 3)、4(n = 6)和 7(n = 9)对大肠杆菌具有极佳的活性,抑菌区分别为 8.1 ± 0.4 毫米、8.3 ± 0.4 毫米和 7.9 ± 0.5 毫米,而扑热息痛和氨苄西林的抑菌区分别为 8.0 ± 0.0 毫米和 16.0 ± 0.0 毫米。有趣的是,化合物 7(n = 9)和 10(n = 14)对金黄色葡萄球菌表现出卓越的活性,抑制区分别为 8.2 ± 0.4 毫米和 12.5 ± 0.5 毫米,优于扑热息痛(6.0 ± 0.0 毫米)和氨苄西林(11.0 ± 0.0 毫米)。体外研究结果与使用 AutoDock Vina 软件对 DNA 回旋酶活性位点进行的分子对接研究结果一致,揭示了该系列化合物的结构-活性关系。此外,合成的化合物 1-10 符合药物相似性分析和 ADME 药代动力学特征。这项研究的结果为通过衍生已知药物扑热息痛而获得的潜在抗菌药物开辟了新的前景。虽然这些初步结果很有希望,但还需要进一步进行毒性和体内分析,以确保新开发药物在生物体内的有效性和安全性,从而更清楚地了解其作为抗菌药物的潜力。
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引用次数: 0
Production and Decomposition of Mangrove Species Rhizophora apiculata Blume in Surabaya East Coast Indonesia 印度尼西亚泗水东海岸红树林树种 Rhizophora apiculata Blume 的产量和分解情况
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-25 DOI: 10.33736/bjrst.5557.2023
H. Purnobasuki, Sitta Amaliyah, Kazutaka Kobayashi
The mangrove ecosystem is supported by the production and decomposition of leaf litter, as well as the release of nutrients into the environment and the neighbouring coastal seas. The release of phosphorus and nitrogen contributes significantly to the improvement of the nutritional values, which benefits marine species and the neighbourhood. In the current study, nutrient release, leaf decomposition rate, litter generation, and mangrove habitat at Surabaya East Coast, Java, Indonesia were all examined. Three transects and three plots in each transect were established. The percentage of initial dry mass remaining in the litter bags were determined by using two sample t-test in Statistica 6.0 software. The decomposition of Rhizophora apiculata leaves was studied by using litter bag technique. They were made of synthetic nylon with the dimension of 15×15×25 cm and mesh size of 1×1.25 mm2. Senescent leaves were used because they present major leaves on the forest floor. According to the findings, daily mangrove litter production (dry weight) varied between 2.15 and 3.28 g/m2. Branch litter (9.43 – 13.27%), reproductive parts (8.20 – 14, 31%), and leaf litter (76.26 – 78.53%) were the other major contributors. The 345.6 ha of mangrove forests along the east coast of Surabaya are the results of reforestation, which has the potential to produce nitrogen and phosphorus at the rates of 109.43 to 173.549 kg/ha/year and 5.467 to 8.12 kg/h/year, respectively. These results imply that decomposition breakdown rates differ across the research area due to the variation in the nutrients availability. Changes in the breakdown of detritus point to variations in nutrient intake, which is crucial for mangrove ecosystems.
红树林生态系统由落叶的产生和分解以及向环境和邻近近海释放养分支撑。磷和氮的释放极大地促进了营养价值的提高,使海洋物种和附近海域受益。本研究对印度尼西亚爪哇岛泗水东海岸的养分释放、叶片分解率、垃圾生成量和红树林生境进行了研究。在每个横断面上建立了三个横断面和三个地块。用 Statistica 6.0 软件中的两样本 t 检验确定了垃圾袋中剩余的初始干质量百分比。使用垃圾袋技术研究了根瘤菌叶片的分解情况。垃圾袋由合成尼龙制成,尺寸为 15×15×25 厘米,网眼大小为 1×1.25 平方毫米。使用衰老叶片是因为它们是森林地面上的主要叶片。研究结果表明,红树林垃圾的日产量(干重)介于 2.15 和 3.28 克/平方米之间。树枝残屑(9.43 - 13.27%)、生殖部分(8.20 - 14.31%)和叶片残屑(76.26 - 78.53%)是其他主要成分。泗水东海岸的 345.6 公顷红树林是植树造林的成果,具有产生氮和磷的潜力,产生率分别为 109.43 至 173.549 千克/公顷/年和 5.467 至 8.12 千克/小时/年。这些结果表明,由于养分供应的不同,整个研究区域的分解率也不尽相同。碎屑分解率的变化表明养分摄入量的变化,这对红树林生态系统至关重要。
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引用次数: 0
Distribution of Commercially Important Edible Mollusc (Bivalvia and Gastropoda) from Six Districts of Terengganu, Malaysia 马来西亚丁加奴六个地区重要商业食用软体动物(双壳类和腹足类)的分布情况
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-25 DOI: 10.33736/bjrst.6001.2023
Nurul Aqilah, Mohd Hanafi Idris Pausi, Muhammad Syahir, AB Lah
Terengganu has a large coastal and wetland area where it provides habitat for edible mollusc. The edible molluscs are one of the most important sources of animal protein for the local communities. The distribution of edible molluscs was studied in six districts of the Terengganu coastal waters between August 2020 and March 2022. In each district, samples were purchased from the wet market, fishing villages and roadside outlets every four months interval. A total of 1,043 individuals was observed and 23 species of edible molluscs were recorded from six districts of Terengganu representing 11 families. Bivalves were found to be dominated by 14 species from seven families. The family Cyrenidae (bivalve) was widespread in five districts where Geloina expansa, was recorded in almost all districts. Meanwhile, for gastropods, nine species from four families were found where Ellobiidae (gastropod) was dominant and the most common gastropod species was Faunus ater, which was found in four locations in Terengganu. These species have great commercial value in Terengganu districts as they are sought extensively as food by the locals and also represent one of their sources of financial survival, especially for those living near to coastal areas. This baseline study could serve as a future indicator for the sustainable management of fisheries resources and for further ecological studies.
丁加奴拥有大片沿海和湿地,为可食用软体动物提供了栖息地。可食用软体动物是当地社区最重要的动物蛋白质来源之一。2020 年 8 月至 2022 年 3 月期间,对丁加奴沿海水域六个区的可食用软体动物分布情况进行了研究。在每个地区,每隔四个月从湿地市场、渔村和路边商店购买一次样本。在丁加奴的六个地区共观察到 1,043 个个体,记录到 23 种可食用软体动物,代表 11 个科。双壳类动物主要有 7 科 14 种。双壳纲鲤科(Cyrenidae)广泛分布于五个地区,其中膨胀鳕科(Geloina expansa)几乎在所有地区都有记录。与此同时,在腹足类动物中,发现了来自 4 个科的 9 个物种,其中以 Ellobiidae(腹足类)为主,最常见的腹足类物种是 Faunus ater,在丁加奴的 4 个地方都有发现。这些物种在丁加奴地区具有很高的商业价值,因为它们被当地人广泛当作食物,也是他们的经济来源之一,尤其是那些生活在沿海地区附近的人。这项基线研究可作为未来渔业资源可持续管理和进一步生态研究的指标。
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引用次数: 0
Analysis of Seven Human Respiratory Coronavirus (CoV) S Proteins from a Bioinformatics Approach 从生物信息学方法分析七种人类呼吸道冠状病毒 (CoV) S 蛋白
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-25 DOI: 10.33736/bjrst.5853.2023
Leonard Whye, Kit Lim, Hui Chung
The coronavirus disease 2019 (COVID-19) has caused a huge pandemic repercussion across the globe and it is mainly contributed by the human severe acute respiratory syndrome coronavirus (SARS-CoV-2). There are seven human respiratory coronaviruses identified to date, namely HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV and SARS-CoV-2. A recently published bioinformatic human CoV comparison only covered four human CoV. Therefore, in this study, a bioinformatics approach-based analyses route was taken to dissect the S proteins of all the available (seven) human respiratory coronaviruses publicly available in the GenBank database. The antigenic epitope amount is postulated to be the most accurate bioindicator among all in determining the severity of a particular human respiratory coronavirus. Other powerful bioinformatic indicators are global similarity index, maximum likelihood phylogenetic analysis as well as domain analysis. The data generated in this study can be channelled to the vaccine and antiviral drug development to combat the current and future spread of the human respiratory coronaviruses.
2019 年冠状病毒病(COVID-19)在全球范围内引起了巨大的流行反响,它主要是由人类严重急性呼吸系统综合征冠状病毒(SARS-CoV-2)引起的。迄今已发现七种人类呼吸道冠状病毒,即 HCoV-229E、HCoV-NL63、HCoV-OC43、HCoV-HKU1、MERS-CoV、SARS-CoV 和 SARS-CoV-2。最近发表的生物信息学人类 CoV 比较只涵盖了四种人类 CoV。因此,本研究采用基于生物信息学方法的分析途径,对 GenBank 数据库中公开的所有(七种)人类呼吸道冠状病毒的 S 蛋白进行了剖析。据推测,抗原表位量是确定特定人类呼吸道冠状病毒严重程度的最准确的生物指标。其他强大的生物信息学指标包括全局相似性指数、最大似然系统发生分析和域分析。这项研究产生的数据可用于疫苗和抗病毒药物的开发,以应对当前和未来人类呼吸道冠状病毒的传播。
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引用次数: 0
Phytochemical Analysis and Antioxidant Activity of Aqueous Extract of Ficus septica Leaves from Sabah, Malaysia 马来西亚沙巴薜荔叶水提取物的植物化学分析和抗氧化活性
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-25 DOI: 10.33736/bjrst.5591.2023
Senty Vun-Sang, MOHAMMAD IQBAL
Medicinal plants have long been used as primary antidotes for a variety of ailments, including tuberculosis, heart diseases, cancer, wound healing, asthma, diabetes mellitus, hypertension, pharyngitis, etc. Medicinal plant of Ficus septica Burm. f. (Moraceae) is a subtropical tree commonly known as the ivory fig, septic fig or white-veined fig. The present work aims to investigate the antioxidant activity, phenolic and flavonoid content, and qualitative screening of various phytochemicals in aqueous extracts of F. septica leaves. Total phenol and flavonoid contents were calculated using Folin-Ciocalteau and aluminium chloride reagents. The antioxidative effect of F. septica was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Ficus septica was found to contain 27.32 ± 0.03 mg/g total phenolics expressed as gallic acid equivalent and 12.65 ± 0.00 mg/g total flavonoid expressed as catechin equivalent. In addition, the leaf extracts were found to contain various secondary metabolites such as alkaloids, flavonoids, tannins, saponins, steroids and triterpenoids. The ability of F. septica to scavenge the DPPH radical was determined by its IC50 value. The IC50 value of F. septica was 4.45 µg/mL. Inferred from the presence of phytochemicals, total phenolic and flavonoid content, and antioxidant activity of the plant, F. septica could be a potential addition to pharmaceutical products to improve human health by participating in the antioxidant defence system against the production of free radicals.
长期以来,药用植物一直被用作治疗肺结核、心脏病、癌症、伤口愈合、哮喘、糖尿病、高血压、咽炎等多种疾病的主要解毒剂。无花果(桑科)是一种亚热带树种,俗称象牙无花果、败酱草无花果或白脉无花果。本研究旨在探讨无花果叶片水提取物中的抗氧化活性、酚类和类黄酮含量以及各种植物化学物质的定性筛选。采用 Folin-Ciocalteau 和氯化铝试剂计算总酚和类黄酮含量。使用 2,2-二苯基-1-苦基肼(DPPH)自由基清除试验评估了七叶树的抗氧化效果。研究发现,七叶树含有 27.32 ± 0.03 mg/g 总酚类(以没食子酸当量表示)和 12.65 ± 0.00 mg/g 总黄酮(以儿茶素当量表示)。此外,还发现叶提取物中含有多种次生代谢物,如生物碱、黄酮类、单宁、皂苷、甾体和三萜类化合物。败酱草清除 DPPH 自由基的能力由其 IC50 值决定。F. septica 的 IC50 值为 4.45 µg/mL。从植物化学物质的存在、总酚类和类黄酮含量以及植物的抗氧化活性推断,败酱草可作为一种潜在的药物添加剂,通过参与抗氧化防御系统来对抗自由基的产生,从而改善人类健康。
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Borneo Journal of Resource Science and Technology
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